pycom05g32980

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0014539
Physical Location & Seq
Reverse (-)
32168644 .. 32171074
2431 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1542 bp
ATGGCCGTGGCGCAGACAGACCCAGCAGCAGCAACGAGAAGACATGATGATGATGAAGATCGGGTGATGATATACTTGCCCTTCTCTAATCGTGTGAGGCTCCAGAAACTACCCCTCCTAACTGATCATCTCTCGCTCGGATCAGATTCAGACCTAGTTCAAAGTAACCTCATCAACACCAACCCCCTAGAACACAACCCCTTCTTCTCCCAGTCCGATTGTTTCTACATAGCCCCATCTGATGTCGTCCTCCGCCACATCATCTACGACTACAAACACCCTCAACCGAAAGCTGCAGCAGCAGGTGGAGGTGGCGGTGAAGAAGCAAAACAAGGCTGCTCTAGTACTACTACTAGTGCCTTGGCATACCACCGCGCCGGCCCACGACGCCAAATCTTGTTCGATGCGTCCAGGATCAAGGCGGCTATTGTGACCTGCGGTGGCCTCTGCCCGGGTCTCAACACCGTCGTCCGAGAGCTTGTTCTTGGCCTCTGGGATCTCTACGGGGTCCGTCACATTTTTGGTATCAAGGCTGGCTACCGTGGCTTTTACTCTACGGAACCCCTCCCACTCAACCCCAAGCTTGTTCACAACTGGCACAAGAACGGCGGCACCCATCTCCAGACCTCCAGAGGCGGTTTTGATCTTCAAAAGATTGTCGATGCCATCCAACGTCGCGGCTATAATCAGTTGTACATCATAGGTGGAGATGGCACGATGCGCGGGGTCGTTGAGATATATCATGAGATATGTCAGCGCAAACTGAATATTGCGGTGACTGGAATTCCAAAAACTGTGGACAATGATGTAGGTATCATCGACAGGTCTTTTGGGTTTCAGACAGCGGTAGAAATGGCACAGCAGGCAATCAGCGCAGCTCATGTGGAGGCGGAAAGTGCAGTGAATGGAATAGGGTTGGTGAAGCTAATGGGTCGAAGTACAGGGCATATTGCTCTACATGCAACACTGAGCAGCCGTGACGTGGACTGCTGCCTAATTCCTGAAACTCCATTTTACCTGGAAGGAAAAGGAGGGCTATTTGAGTTCCTTGAGCAGCGATTGAGGGAGAATGGACACGCAGTGTTAGTAGTGGCTGAGGGAGCTGGACAGGATCAAATGATCGAGGCTAATAACAATGATCATCATGCTGATAATAATAAGGATGAATCAGGGAACCCAGTGTTCCTTGACGTTGGGCAGTGGTTGAAGTCGGAGCTAAACAAGTGGTGGAAGAGGGAGCACCCAAATGAGCTCTTCACTGTCAAGTACATAGATCCGACATACATGATACGCGCGGTCGCTGCAAATTCAACAGATAACTTGTACTGCACACTGGTGGCACATTCAGCAATCCACGGAGTCATGGCAGGTTATACTGGATTTGTGTCTGGCCCCATCAACGGAAACTATGCTTACATTCCACTGGAAGAGGTTGCCAAGGCTAAAAATGAAGTCGACACGAGAGACCACAAATGGGCCTGGGTTAGATCGGTCACCAACCAGCCTGATTTTCATATATACAGCAACGACGACAAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

514

Amino Acids

56.72

Weight (kDa)

6.12

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 141 - 452 5.4e-55 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47810
fragaria_vesca FvH4_3g00180
malus_domestica MD05G1363600.v1.1
prunus_persica Prupe.4G002000_v2.0.a1
pyrus_communis pycom05g32980
rosa_chinensis RchiOBHm_Chr5g0000241
rosa_laevigata RLG00000030852
rosa_multiflora Rmu_co8387729.1_g000001 Rmu_sc0003320.1_g000002 Rmu_sc0041897.1_g000001
rosa_roxburghii Rroxscaffold_1G00075860
rosa_rugosa Rorug04G0382700
rosa_samantha Rh5AG001700 Rh5BG003100 Rh5CG002000 Rh5DG002000
rosa_wichuraiana Rw5G000220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 293
Acc36I ACCTGC 3 cut(s) 293, 443, 1358
AccB1I GGYRCC 1 cut(s) 611
AccI GTMKAC 1 cut(s) 1455
AccII CGCG 5 cut(s) 375, 678, 723, 1293, 1295
AclWI GGATC 5 cut(s) 148, 422, 504, 1119, 1268
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 783, 1306
AcyI GRCGYC 1 cut(s) 388
AdeI CACNNNGTG 1 cut(s) 1081
AfaI GTAC 5 cut(s) 346, 695, 940, 1268, 1325
AfiI CCNNNNNNNGG 4 cut(s) 451, 982, 1400, 1474
AgsI TTSAA 4 cut(s) 161, 650, 1207, 1311
AhlI ACTAGT 1 cut(s) 353
AjiI CACGTC 1 cut(s) 982
AjnI CCWGG 3 cut(s) 410, 1017, 1478
AleI CACNNNNGTG 1 cut(s) 1334
AluBI AGCT 8 cut(s) 293, 478, 583, 878, 925, 1103, 1216, 1252
AluI AGCT 8 cut(s) 293, 478, 583, 878, 925, 1103, 1216, 1252
Alw21I GWGCWC 2 cut(s) 1242, 1254
Alw26I GTCTC 2 cut(s) 461, 1458
AlwI GGATC 5 cut(s) 148, 422, 504, 1119, 1268
Ama87I CYCGRG 1 cut(s) 451
AoxI GGCC 6 cut(s) 3, 379, 442, 487, 1390, 1476
ApoI RAATTY 2 cut(s) 783, 1306
ArsI GACNNNNNNTTYG 2 cut(s) 812, 844
AspLEI GCGC 6 cut(s) 13, 377, 723, 759, 875, 1295
AspS9I GGNCC 4 cut(s) 380, 508, 1391, 1476
AsuC2I CCSGG 2 cut(s) 452, 453
AsuHPI GGTGA 5 cut(s) 76, 329, 787, 931, 1486
AvaI CYCGRG 1 cut(s) 451
AvaII GGWCC 1 cut(s) 508
BanI GGYRCC 1 cut(s) 611
BanII GRGCYC 1 cut(s) 1254
BauI CACGAG 1 cut(s) 1459
BbsI GAAGAC 1 cut(s) 46
Bbv12I GWGCWC 2 cut(s) 1242, 1254
BbvCI CCTCAGC 1 cut(s) 1095
BccI CCATC 5 cut(s) 244, 624, 674, 704, 1403
BceAI ACGGC 2 cut(s) 622, 960
BciT130I CCWGG 3 cut(s) 412, 1019, 1480
BclI TGATCA 2 cut(s) 124, 1138
BcnI CCSGG 2 cut(s) 452, 453
BcoDI GTCTC 2 cut(s) 461, 1458
BcuI ACTAGT 1 cut(s) 353
BfaI CTAG 4 cut(s) 155, 188, 342, 354
BfmI CTRYAG 1 cut(s) 294
BfuAI ACCTGC 3 cut(s) 293, 443, 1358
BmcAI AGTACT 1 cut(s) 346
Bme1390I CCNGG 5 cut(s) 412, 452, 453, 1019, 1480
Bme18I GGWCC 1 cut(s) 508
BmeT110I CYCGRG 1 cut(s) 451
BmgBI CACGTC 1 cut(s) 982
BmgT120I GGNCC 4 cut(s) 380, 508, 1391, 1476
BmiI GGNNCC 6 cut(s) 101, 509, 561, 613, 1175, 1393
BmrFI CCNGG 5 cut(s) 412, 452, 453, 1019, 1480
BmrI ACTGGG 2 cut(s) 205, 1172
BmsI GCATC 3 cut(s) 394, 652, 708
BmuI ACTGGG 2 cut(s) 205, 1172
BpiI GAAGAC 1 cut(s) 46
BpmI CTGGAG 3 cut(s) 86, 605, 613
Bpu10I CCTNAGC 1 cut(s) 1095
BpuEI CTTGAG 1 cut(s) 1070
BpuMI CCSGG 2 cut(s) 452, 453
BsaBI GATNNNNATC 1 cut(s) 57
BsaHI GRCGYC 1 cut(s) 388
BsaI GGTCTC 2 cut(s) 461, 1458
BsaJI CCNNGG 7 cut(s) 6, 360, 451, 541, 1354, 1437, 1479
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 4 cut(s) 451, 982, 1400, 1474
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 7 cut(s) 211, 599, 784, 1178, 1338, 1381, 1428
Bse8I GATNNNNATC 1 cut(s) 57
BseBI CCWGG 3 cut(s) 412, 1019, 1480
BseDI CCNNGG 7 cut(s) 6, 360, 451, 541, 1354, 1437, 1479
BseGI GGATG 2 cut(s) 666, 1168
BseJI GATNNNNATC 1 cut(s) 57
BseLI CCNNNNNNNGG 4 cut(s) 451, 982, 1400, 1474
BseMII CTCAG 2 cut(s) 959, 1086
BseNI ACTGG 7 cut(s) 211, 599, 784, 1178, 1338, 1381, 1428
BseYI CCCAGC 1 cut(s) 22
BsgI GTGCAG 2 cut(s) 918, 1312
Bsh1236I CGCG 5 cut(s) 375, 678, 723, 1293, 1295
Bsh1285I CGRYCG 1 cut(s) 1299
BshFI GGCC 6 cut(s) 5, 381, 444, 489, 1392, 1478
BshNI GGYRCC 1 cut(s) 611
BsiEI CGRYCG 1 cut(s) 1299
BsiHKAI GWGCWC 2 cut(s) 1242, 1254
BsiHKCI CYCGRG 1 cut(s) 451
BsiSI CCGG 2 cut(s) 378, 452
BslI CCNNNNNNNGG 4 cut(s) 451, 982, 1400, 1474
BsmAI GTCTC 2 cut(s) 461, 1458
BsnI GGCC 6 cut(s) 5, 381, 444, 489, 1392, 1478
Bso31I GGTCTC 2 cut(s) 461, 1458
BsoBI CYCGRG 1 cut(s) 451
Bsp1286I GDGCHC 2 cut(s) 1242, 1254
Bsp1407I TGTACA 1 cut(s) 693
BspANI GGCC 6 cut(s) 5, 381, 444, 489, 1392, 1478
BspCNI CTCAG 2 cut(s) 960, 1087
BspFNI CGCG 5 cut(s) 375, 678, 723, 1293, 1295
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 6 cut(s) 101, 509, 561, 613, 1175, 1393
BspMAI CTGCAG 1 cut(s) 298
BspMI ACCTGC 3 cut(s) 293, 443, 1358
BspPI GGATC 5 cut(s) 148, 422, 504, 1119, 1268
BspQI GCTCTTC 1 cut(s) 1259
BspT107I GGYRCC 1 cut(s) 611
BspTNI GGTCTC 2 cut(s) 461, 1458
BsrFI RCCGGY 1 cut(s) 377
BsrGI TGTACA 1 cut(s) 693
BsrI ACTGG 7 cut(s) 211, 599, 784, 1178, 1338, 1381, 1428
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 7 cut(s) 6, 360, 451, 541, 1354, 1437, 1479
BssNI GRCGYC 1 cut(s) 388
BssSI CACGAG 1 cut(s) 1459
BssT1I CCWWGG 2 cut(s) 360, 1437
Bst2BI CACGAG 1 cut(s) 1459
Bst2UI CCWGG 3 cut(s) 412, 1019, 1480
Bst4CI ACNGT 4 cut(s) 466, 542, 796, 1261
Bst6I CTCTTC 3 cut(s) 1226, 1259, 1422
BstACI GRCGYC 1 cut(s) 388
BstAUI TGTACA 1 cut(s) 693
BstC8I GCNNGC 3 cut(s) 379, 535, 864
BstDEI CTNAG 2 cut(s) 968, 1095
BstDSI CCRYGG 3 cut(s) 6, 541, 1354
BstEII GGTNACC 1 cut(s) 1492
BstF5I GGATG 2 cut(s) 666, 1168
BstFNI CGCG 5 cut(s) 375, 678, 723, 1293, 1295
BstHHI GCGC 6 cut(s) 13, 377, 723, 759, 875, 1295
BstMAI GTCTC 2 cut(s) 461, 1458
BstMCI CGRYCG 1 cut(s) 1299
BstNI CCWGG 3 cut(s) 412, 1019, 1480
BstNSI RCATGY 1 cut(s) 962
BstPI GGTNACC 1 cut(s) 1492
BstSCI CCNGG 5 cut(s) 410, 450, 451, 1017, 1478
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 5 cut(s) 375, 678, 723, 1293, 1295
BstV2I GAAGAC 1 cut(s) 46
BstX2I RGATCY 2 cut(s) 496, 1273
BstYI RGATCY 2 cut(s) 496, 1273
BsuRI GGCC 6 cut(s) 5, 381, 444, 489, 1392, 1478
BtgI CCRYGG 3 cut(s) 6, 541, 1354
BtrI CACGTC 1 cut(s) 982
BtsCI GGATG 2 cut(s) 666, 1168
BtsI GCAGTG 3 cut(s) 906, 1086, 1205
BtsIMutI CAGTG 8 cut(s) 906, 965, 1086, 1185, 1205, 1257, 1331, 1421
BveI ACCTGC 3 cut(s) 293, 443, 1358
Cac8I GCNNGC 3 cut(s) 379, 535, 864
CciI TCATGA 1 cut(s) 742
CfoI GCGC 6 cut(s) 13, 377, 723, 759, 875, 1295
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 4 cut(s) 380, 508, 1391, 1476
Cfr9I CCCGGG 1 cut(s) 451
CseI GACGC 2 cut(s) 396, 396
Csp6I GTAC 5 cut(s) 345, 694, 939, 1267, 1324
CspCI CAANNNNNGTGG 2 cut(s) 777, 812
CviAII CATG 7 cut(s) 44, 743, 881, 959, 1145, 1285, 1363
CviQI GTAC 5 cut(s) 345, 694, 939, 1267, 1324
DdeI CTNAG 2 cut(s) 968, 1095
DraIII CACNNNGTG 1 cut(s) 1081
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 1226, 1259, 1422
EarI CTCTTC 3 cut(s) 1226, 1259, 1422
EciI GGCGGA 2 cut(s) 242, 905
Ecl136II GAGCTC 1 cut(s) 1252
Eco130I CCWWGG 2 cut(s) 360, 1437
Eco24I GRGCYC 1 cut(s) 1254
Eco31I GGTCTC 2 cut(s) 461, 1458
Eco47I GGWCC 1 cut(s) 508
Eco53kI GAGCTC 1 cut(s) 1252
Eco88I CYCGRG 1 cut(s) 451
Eco91I GGTNACC 1 cut(s) 1492
EcoICRI GAGCTC 1 cut(s) 1252
EcoO65I GGTNACC 1 cut(s) 1492
EcoRI GAATTC 1 cut(s) 783
EcoRII CCWGG 3 cut(s) 410, 1017, 1478
EcoT14I CCWWGG 2 cut(s) 360, 1437
EcoT38I GRGCYC 1 cut(s) 1254
ErhI CCWWGG 2 cut(s) 360, 1437
FaeI CATG 7 cut(s) 47, 746, 884, 962, 1148, 1288, 1366
FatI CATG 7 cut(s) 43, 742, 880, 958, 1144, 1284, 1362
FauI CCCGC 1 cut(s) 716
FbaI TGATCA 2 cut(s) 124, 1138
FblI GTMKAC 1 cut(s) 1455
FokI GGATG 2 cut(s) 653, 1175
FriOI GRGCYC 1 cut(s) 1254
FspBI CTAG 4 cut(s) 155, 188, 342, 354
GlaI GCGC 6 cut(s) 12, 376, 722, 758, 874, 1294
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 3 cut(s) 86, 605, 613
HaeIII GGCC 6 cut(s) 5, 381, 444, 489, 1392, 1478
HapII CCGG 2 cut(s) 378, 452
HgaI GACGC 2 cut(s) 396, 396
HhaI GCGC 6 cut(s) 13, 377, 723, 759, 875, 1295
Hin1I GRCGYC 1 cut(s) 388
Hin1II CATG 7 cut(s) 47, 746, 884, 962, 1148, 1288, 1366
Hin6I GCGC 6 cut(s) 11, 375, 721, 757, 873, 1293
HinP1I GCGC 6 cut(s) 11, 375, 721, 757, 873, 1293
HincII GTYRAC 1 cut(s) 1456
HindII GTYRAC 1 cut(s) 1456
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 3 cut(s) 146, 1166, 1359
HpaII CCGG 2 cut(s) 378, 452
HphI GGTGA 5 cut(s) 76, 329, 787, 931, 1486
Hpy166II GTNNAC 4 cut(s) 589, 799, 985, 1456
Hpy188I TCNGA 9 cut(s) 140, 145, 151, 217, 241, 473, 840, 1213, 1278
Hpy188III TCNNGA 5 cut(s) 103, 622, 630, 743, 1001
Hpy8I GTNNAC 4 cut(s) 589, 799, 985, 1456
Hpy99I CGWCG 4 cut(s) 390, 470, 678, 1532
HpyAV CCTTC 3 cut(s) 91, 211, 1016
HpyCH4III ACNGT 4 cut(s) 466, 542, 796, 1261
HpyCH4IV ACGT 3 cut(s) 673, 981, 1191
HpyCH4V TGCA 5 cut(s) 296, 899, 962, 1304, 1329
HpyF3I CTNAG 2 cut(s) 968, 1095
HpySE526I ACGT 3 cut(s) 673, 981, 1191
Hsp92I GRCGYC 1 cut(s) 388
Hsp92II CATG 7 cut(s) 47, 746, 884, 962, 1148, 1288, 1366
HspAI GCGC 6 cut(s) 11, 375, 721, 757, 873, 1293
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
Ksp22I TGATCA 2 cut(s) 124, 1138
LguI GCTCTTC 1 cut(s) 1259
LmnI GCTCC 4 cut(s) 105, 1100, 1213, 1237
LweI GCATC 3 cut(s) 394, 652, 708
MaeI CTAG 4 cut(s) 155, 188, 342, 354
MaeII ACGT 3 cut(s) 673, 981, 1191
MaeIII GTNAC 6 cut(s) 164, 430, 512, 775, 977, 1492
MboII GAAGA 8 cut(s) 51, 68, 196, 332, 638, 1243, 1246, 1439
MflI RGATCY 2 cut(s) 496, 1273
MhlI GDGCHC 2 cut(s) 1242, 1254
MluCI AATT 3 cut(s) 783, 996, 1306
MlyI GAGTC 1 cut(s) 1368
MmeI TCCRAC 3 cut(s) 694, 1191, 1301
MroNI GCCGGC 1 cut(s) 377
MslI CAYNNNNRTG 3 cut(s) 48, 1245, 1334
MspA1I CMGCKG 1 cut(s) 845
MspI CCGG 2 cut(s) 378, 452
MspR9I CCNGG 5 cut(s) 412, 452, 453, 1019, 1480
MvaI CCWGG 3 cut(s) 412, 1019, 1480
MvnI CGCG 5 cut(s) 375, 678, 723, 1293, 1295
NaeI GCCGGC 1 cut(s) 379
NciI CCSGG 2 cut(s) 452, 453
NgoMIV GCCGGC 1 cut(s) 377
NlaIII CATG 7 cut(s) 47, 746, 884, 962, 1148, 1288, 1366
NlaIV GGNNCC 6 cut(s) 101, 509, 561, 613, 1175, 1393
NmuCI GTSAC 5 cut(s) 430, 512, 775, 977, 1492
NspI RCATGY 1 cut(s) 962
OliI CACNNNNGTG 1 cut(s) 1334
PagI TCATGA 1 cut(s) 742
PaqCI CACCTGC 1 cut(s) 293
PciSI GCTCTTC 1 cut(s) 1259
PdiI GCCGGC 1 cut(s) 379
PfeI GAWTC 2 cut(s) 146, 1166
PfoI TCCNGGA 1 cut(s) 410
PleI GAGTC 1 cut(s) 1367
PpsI GAGTC 1 cut(s) 1367
Psp124BI GAGCTC 1 cut(s) 1254
Psp6I CCWGG 3 cut(s) 410, 1017, 1478
PspEI GGTNACC 1 cut(s) 1492
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 3 cut(s) 410, 1017, 1478
PspN4I GGNNCC 6 cut(s) 101, 509, 561, 613, 1175, 1393
PspPI GGNCC 4 cut(s) 380, 508, 1391, 1476
PstI CTGCAG 1 cut(s) 298
PsuI RGATCY 2 cut(s) 496, 1273
RsaI GTAC 5 cut(s) 346, 695, 940, 1268, 1325
RsaNI GTAC 5 cut(s) 345, 694, 939, 1267, 1324
RseI CAYNNNNRTG 3 cut(s) 48, 1245, 1334
SacI GAGCTC 1 cut(s) 1254
SalI GTCGAC 1 cut(s) 1454
SapI GCTCTTC 1 cut(s) 1259
Sau96I GGNCC 4 cut(s) 380, 508, 1391, 1476
ScaI AGTACT 1 cut(s) 346
SchI GAGTC 1 cut(s) 1368
ScrFI CCNGG 5 cut(s) 412, 452, 453, 1019, 1480
SduI GDGCHC 2 cut(s) 1242, 1254
SfaNI GCATC 3 cut(s) 394, 652, 708
SfcI CTRYAG 1 cut(s) 294
SinI GGWCC 1 cut(s) 508
SmaI CCCGGG 1 cut(s) 453
SmiMI CAYNNNNRTG 3 cut(s) 48, 1245, 1334
SmlI CTYRAG 1 cut(s) 1049
SmoI CTYRAG 1 cut(s) 1049
SpeI ACTAGT 1 cut(s) 353
Sse9I AATT 3 cut(s) 783, 996, 1306
SspI AATATT 1 cut(s) 769
SspMI CTAG 4 cut(s) 155, 188, 342, 354
SstI GAGCTC 1 cut(s) 1254
StyD4I CCNGG 5 cut(s) 410, 450, 451, 1017, 1478
StyI CCWWGG 2 cut(s) 360, 1437
TaaI ACNGT 4 cut(s) 466, 542, 796, 1261
TaiI ACGT 3 cut(s) 676, 984, 1194
TaqI TCGA 6 cut(s) 402, 660, 819, 934, 1122, 1455
TaqII GACCGA 1 cut(s) 1480
TasI AATT 3 cut(s) 783, 996, 1306
TatI WGTACW 5 cut(s) 344, 693, 938, 1266, 1323
TauI GCSGC 3 cut(s) 425, 612, 681
TfiI GAWTC 2 cut(s) 146, 1166
TscAI CASTG 8 cut(s) 906, 972, 1086, 1185, 1205, 1264, 1338, 1428
TseFI GTSAC 5 cut(s) 430, 512, 775, 977, 1492
Tsp45I GTSAC 5 cut(s) 430, 512, 775, 977, 1492
TspDTI ATGAA 4 cut(s) 69, 1179, 1464, 1502
TspGWI ACGGA 4 cut(s) 500, 572, 1371, 1416
TspMI CCCGGG 1 cut(s) 451
TspRI CASTG 8 cut(s) 906, 972, 1086, 1185, 1205, 1264, 1338, 1428
VpaK11BI GGWCC 1 cut(s) 508
XapI RAATTY 2 cut(s) 783, 1306
XceI RCATGY 1 cut(s) 962
XmaI CCCGGG 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 1455
XspI CTAG 4 cut(s) 155, 188, 342, 354
ZrmI AGTACT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.