pycom06g05350

Maspardin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012477
Physical Location & Seq
Reverse (-)
7741568 .. 7746061
4494 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1236 bp
ATGACAAGGGGAGGTTTTGGGGGTAGAAAACCAGCAATGAAAGGCGTCTACTCGCCGCCCGGAGATTACATCTACTTCAGGTCCCAAGTCCCTCTTCACAAGATCCCAATTGGCACGAAGCAGTGGCGATACTATGATTTTGGCCCAAAAGCAGTACCTCCTCTTATTTGTCTTCCTGGATCAGCAGGGACAGCTGATGTCTGCTACAAGCAGATCATGCCATTGTCCATGAAGGGTTATCGGGTGATTTCTGTTGATATTCCAAGTGTATGGAATCATCACGAGTGGATTCTAGCATTTGAGAAATTCTTGGATGCTATTGATGTCCATCACATACATATCTATGGTACAGCCCTTGGGGGCTTCTTGGCTCAACTTTTTGCTCAGCATCGTCCAAGAAGAGTTCGGTCATTGATACTATCAAACACATTTTTGGACACGCGTAACTTCTCTGCAGAAATGCCATGGGCTCCCGTCATTAATTGGACCCCTTCTTTTTTGTTGAAAAGATATGTCTTGACCGGAATACTCGATGGCCCCCATGAGCCATTTATTGCAGATTCAGTCGACTTTGTTGTTTCTCAGGTTGAGATGCTCTCAAGAGAGGACCTCGCCTCCAGGTTAACTTTAACCGCTGATACTGTTTCAGTTGGACCGCTGTTGCTTACAGATTCATTCATTACTATAATGGATACAAATGACTACAGCGGGATTCCTCAACGACTCAAAGATGAAGTTAGCGAAAGGTATCTTAACGCAAGGCAAGCACAATTGAAGACTGGGGGAGATTTTCCGTTCCTGTCACGACCAGATGAAGTTAACTTACATCTTCAGCTGCATCTAAGACGGGTTGGTGTGGAACCTCGACCTGACTTAGTTCCAGGTATTTCGAAGGGAGGTGGCGGTGGTAGTTCTAGCGAAGAGAGTGAGAAAAAAGACCCAGAAGATCCACCCAATGATGATAAAGGAAGCCTGGATAATCCATTTGCAGAACCTCAGCTACCTCCTGCTCCAGAGAGTTCAGAATCTCATAGCTCAGAAGACCAGCTCATCAGCAATGCAAAATTCTGCCACTTCGGTGATGGAGACACGACCTTGAAGAATCAACACACTATAACCACTGAATTTGTTATGCGACTTGGTCGTGAGATGTTTTCTGTTCATTTGCTTTCATTTTATATGGCATCGTTGTACATTAGTTTGAACTGTAATCTGAAGCTCAGACCTGTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

412

Amino Acids

45.94

Weight (kDa)

6.16

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 53 - 172 5.1e-11 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12230
fragaria_vesca FvH4_5g00640 FvH4_5g00640 FvH4_5g00640
malus_domestica MD04G1079900.v1.1 MD06G1074900.v1.1
prunus_persica Prupe.5G079200_v2.0.a1
pyrus_communis pycom04g07200 pycom06g05350
rosa_chinensis RchiOBHm_Chr7g0201621
rosa_laevigata RLG00000003661
rosa_multiflora Rmu_sc0004811.1_g000001
rosa_roxburghii Rroxscaffold_3G00255190
rosa_rugosa Rorug07G0065200
rosa_samantha Rh7AG191900 Rh7BG194100 Rh7CG203100 Rh7DG197100
rosa_wichuraiana Rw7G016830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 48, 567
AccII CGCG 1 cut(s) 442
AciI CCGC 5 cut(s) 56, 633, 656, 708, 903
AclWI GGATC 3 cut(s) 97, 187, 941
AcsI RAATTY 3 cut(s) 305, 1064, 1124
AcuI CTGAAG 3 cut(s) 61, 815, 1235
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 3 cut(s) 156, 349, 1193
AflIII ACRYGT 1 cut(s) 440
AgsI TTSAA 4 cut(s) 505, 775, 1099, 1204
AjnI CCWGG 4 cut(s) 175, 617, 880, 972
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AleI CACNNNNGTG 1 cut(s) 1077
AluBI AGCT 6 cut(s) 194, 835, 1000, 1035, 1048, 1219
AluI AGCT 6 cut(s) 194, 835, 1000, 1035, 1048, 1219
Alw26I GTCTC 1 cut(s) 1080
AlwI GGATC 3 cut(s) 97, 187, 941
AoxI GGCC 2 cut(s) 142, 535
ApeKI GCWGC 1 cut(s) 835
ApoI RAATTY 3 cut(s) 305, 1064, 1124
AseI ATTAAT 1 cut(s) 480
AspS9I GGNCC 6 cut(s) 81, 143, 486, 536, 607, 653
AsuC2I CCSGG 1 cut(s) 60
AsuHPI GGTGA 2 cut(s) 256, 1091
AsuII TTCGAA 1 cut(s) 890
AvaII GGWCC 4 cut(s) 81, 486, 607, 653
BanII GRGCYC 1 cut(s) 472
BarI GAAGNNNNNNTAC 2 cut(s) 807, 839
BauI CACGAG 1 cut(s) 281
BbsI GAAGAC 3 cut(s) 164, 782, 1047
BbvCI CCTCAGC 1 cut(s) 996
BbvI GCAGC 1 cut(s) 822
BccI CCATC 3 cut(s) 336, 527, 1076
BciT130I CCWGG 4 cut(s) 177, 619, 882, 974
BciVI GTATCC 1 cut(s) 685
BcnI CCSGG 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 1080
BfaI CTAG 2 cut(s) 293, 915
BfmI CTRYAG 2 cut(s) 453, 703
BfuI GTATCC 1 cut(s) 685
BisI GCNGC 2 cut(s) 56, 836
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 2 cut(s) 57, 837
Bme1390I CCNGG 5 cut(s) 60, 177, 619, 882, 974
Bme18I GGWCC 4 cut(s) 81, 486, 607, 653
BmgT120I GGNCC 6 cut(s) 81, 143, 486, 536, 607, 653
BmiI GGNNCC 5 cut(s) 83, 471, 488, 538, 861
BmrFI CCNGG 5 cut(s) 60, 177, 619, 882, 974
BmrI ACTGGG 1 cut(s) 789
BmsI GCATC 5 cut(s) 304, 397, 582, 847, 1193
BmuI ACTGGG 1 cut(s) 789
BpiI GAAGAC 3 cut(s) 164, 782, 1047
BplI GAGNNNNNCTC 4 cut(s) 581, 613, 594, 626
BpmI CTGGAG 2 cut(s) 601, 996
Bpu10I CCTNAGC 1 cut(s) 996
Bpu1102I GCTNAGC 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 890
BpuEI CTTGAG 1 cut(s) 583
BpuMI CCSGG 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 327
BsaHI GRCGYC 1 cut(s) 45
BsaJI CCNNGG 2 cut(s) 355, 464
BsaWI WCCGGW 1 cut(s) 521
BsaXI ACNNNNNCTCC 2 cut(s) 599, 629
Bse1I ACTGG 1 cut(s) 784
Bse3DI GCAATG 2 cut(s) 42, 1063
Bse8I GATNNNNATC 1 cut(s) 327
BseBI CCWGG 4 cut(s) 177, 619, 882, 974
BseDI CCNNGG 2 cut(s) 355, 464
BseGI GGATG 1 cut(s) 319
BseJI GATNNNNATC 1 cut(s) 327
BseMI GCAATG 2 cut(s) 42, 1063
BseMII CTCAG 5 cut(s) 398, 596, 1010, 1050, 1234
BseNI ACTGG 1 cut(s) 784
BseRI GAGGAG 1 cut(s) 150
BseXI GCAGC 1 cut(s) 822
Bsh1236I CGCG 1 cut(s) 442
BshFI GGCC 2 cut(s) 144, 537
BsiSI CCGG 2 cut(s) 60, 522
BslFI GGGAC 3 cut(s) 67, 74, 202
BsmAI GTCTC 1 cut(s) 1080
BsmFI GGGAC 3 cut(s) 67, 74, 202
BsnI GGCC 2 cut(s) 144, 537
Bsp119I TTCGAA 1 cut(s) 890
Bsp1286I GDGCHC 1 cut(s) 472
Bsp1407I TGTACA 1 cut(s) 1191
Bsp143I GATC 4 cut(s) 102, 179, 213, 946
Bsp1720I GCTNAGC 1 cut(s) 384
Bsp19I CCATGG 1 cut(s) 464
BspACI CCGC 5 cut(s) 56, 633, 656, 708, 903
BspANI GGCC 2 cut(s) 144, 537
BspCNI CTCAG 5 cut(s) 397, 595, 1009, 1049, 1233
BspFNI CGCG 1 cut(s) 442
BspLI GGNNCC 5 cut(s) 83, 471, 488, 538, 861
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 3 cut(s) 97, 187, 941
BspT104I TTCGAA 1 cut(s) 890
BsrDI GCAATG 2 cut(s) 42, 1063
BsrGI TGTACA 1 cut(s) 1191
BsrI ACTGG 1 cut(s) 784
BssECI CCNNGG 2 cut(s) 355, 464
BssMI GATC 4 cut(s) 102, 179, 213, 946
BssNI GRCGYC 1 cut(s) 45
BssSI CACGAG 1 cut(s) 281
BssT1I CCWWGG 2 cut(s) 355, 464
Bst2BI CACGAG 1 cut(s) 281
Bst2UI CCWGG 4 cut(s) 177, 619, 882, 974
Bst4CI ACNGT 2 cut(s) 643, 1208
Bst6I CTCTTC 3 cut(s) 99, 394, 915
BstACI GRCGYC 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 217
BstAUI TGTACA 1 cut(s) 1191
BstBI TTCGAA 1 cut(s) 890
BstC8I GCNNGC 1 cut(s) 765
BstDEI CTNAG 7 cut(s) 384, 582, 842, 874, 996, 1036, 1220
BstDSI CCRYGG 1 cut(s) 464
BstF5I GGATG 1 cut(s) 319
BstFNI CGCG 1 cut(s) 442
BstKTI GATC 4 cut(s) 105, 182, 216, 949
BstMAI GTCTC 1 cut(s) 1080
BstMBI GATC 4 cut(s) 102, 179, 213, 946
BstMWI GCNNNNNNNGC 3 cut(s) 191, 217, 764
BstNI CCWGG 4 cut(s) 177, 619, 882, 974
BstSCI CCNGG 5 cut(s) 58, 175, 617, 880, 972
BstSFI CTRYAG 2 cut(s) 453, 703
BstUI CGCG 1 cut(s) 442
BstV1I GCAGC 1 cut(s) 822
BstV2I GAAGAC 3 cut(s) 164, 782, 1047
BstX2I RGATCY 2 cut(s) 102, 946
BstXI CCANNNNNNTGG 1 cut(s) 270
BstYI RGATCY 2 cut(s) 102, 946
BsuI GTATCC 1 cut(s) 685
BsuRI GGCC 2 cut(s) 144, 537
BtgI CCRYGG 1 cut(s) 464
BtsCI GGATG 1 cut(s) 319
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 2 cut(s) 128, 1119
Cac8I GCNNGC 1 cut(s) 765
Cfr13I GGNCC 6 cut(s) 81, 143, 486, 536, 607, 653
CseI GACGC 1 cut(s) 34
Csp6I GTAC 3 cut(s) 155, 348, 1192
CspCI CAANNNNNGTGG 2 cut(s) 1108, 1143
CviAII CATG 4 cut(s) 217, 229, 465, 542
CviQI GTAC 3 cut(s) 155, 348, 1192
DdeI CTNAG 7 cut(s) 384, 582, 842, 874, 996, 1036, 1220
DpnI GATC 4 cut(s) 104, 181, 215, 948
DpnII GATC 4 cut(s) 102, 179, 213, 946
Eam1104I CTCTTC 3 cut(s) 99, 394, 915
EarI CTCTTC 3 cut(s) 99, 394, 915
Eco130I CCWWGG 2 cut(s) 355, 464
Eco24I GRGCYC 1 cut(s) 472
Eco47I GGWCC 4 cut(s) 81, 486, 607, 653
Eco57I CTGAAG 3 cut(s) 61, 815, 1235
EcoO109I RGGNCCY 2 cut(s) 81, 607
EcoRII CCWGG 4 cut(s) 175, 617, 880, 972
EcoT14I CCWWGG 2 cut(s) 355, 464
EcoT38I GRGCYC 1 cut(s) 472
ErhI CCWWGG 2 cut(s) 355, 464
FaeI CATG 4 cut(s) 220, 232, 468, 545
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FaqI GGGAC 3 cut(s) 67, 74, 202
FatI CATG 4 cut(s) 216, 228, 464, 541
FauI CCCGC 1 cut(s) 701
FblI GTMKAC 2 cut(s) 48, 567
Fnu4HI GCNGC 2 cut(s) 56, 836
FokI GGATG 1 cut(s) 326
FriOI GRGCYC 1 cut(s) 472
Fsp4HI GCNGC 2 cut(s) 56, 836
FspBI CTAG 2 cut(s) 293, 915
GluI GCNGC 2 cut(s) 56, 836
GsuI CTGGAG 2 cut(s) 601, 996
HaeIII GGCC 2 cut(s) 144, 537
HapII CCGG 2 cut(s) 60, 522
HgaI GACGC 1 cut(s) 34
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 4 cut(s) 220, 232, 468, 545
HincII GTYRAC 3 cut(s) 568, 624, 820
HindII GTYRAC 3 cut(s) 568, 624, 820
HinfI GANTC 8 cut(s) 274, 289, 560, 671, 712, 723, 1025, 1102
HpaI GTTAAC 2 cut(s) 624, 820
HpaII CCGG 2 cut(s) 60, 522
HphI GGTGA 2 cut(s) 256, 1091
Hpy166II GTNNAC 4 cut(s) 49, 568, 624, 820
Hpy188I TCNGA 4 cut(s) 1024, 1039, 1215, 1223
Hpy188III TCNNGA 6 cut(s) 281, 517, 600, 804, 1013, 1145
Hpy8I GTNNAC 4 cut(s) 49, 568, 624, 820
HpyAV CCTTC 3 cut(s) 226, 501, 886
HpyCH4III ACNGT 2 cut(s) 643, 1208
HpyCH4V TGCA 5 cut(s) 455, 557, 838, 989, 1061
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 217, 764
HpyF3I CTNAG 7 cut(s) 384, 582, 842, 874, 996, 1036, 1220
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 4 cut(s) 220, 232, 468, 545
KspAI GTTAAC 2 cut(s) 624, 820
Kzo9I GATC 4 cut(s) 102, 179, 213, 946
LmnI GCTCC 2 cut(s) 475, 1015
Lsp1109I GCAGC 1 cut(s) 822
LweI GCATC 5 cut(s) 304, 397, 582, 847, 1193
MaeI CTAG 2 cut(s) 293, 915
MaeIII GTNAC 2 cut(s) 443, 801
MalI GATC 4 cut(s) 104, 181, 215, 948
MboI GATC 4 cut(s) 102, 179, 213, 946
MboII GAAGA 9 cut(s) 86, 164, 411, 787, 821, 932, 956, 1052, 1111
MfeI CAATTG 2 cut(s) 108, 770
MflI RGATCY 2 cut(s) 102, 946
MhlI GDGCHC 1 cut(s) 472
MluCI AATT 6 cut(s) 108, 305, 481, 770, 1064, 1124
MluI ACGCGT 1 cut(s) 440
MlyI GAGTC 1 cut(s) 717
MmeI TCCRAC 1 cut(s) 631
MseI TTAA 5 cut(s) 480, 623, 629, 753, 819
MslI CAYNNNNRTG 2 cut(s) 342, 1077
MspA1I CMGCKG 5 cut(s) 194, 635, 658, 708, 835
MspI CCGG 2 cut(s) 60, 522
MspR9I CCNGG 5 cut(s) 60, 177, 619, 882, 974
MunI CAATTG 2 cut(s) 108, 770
MvaI CCWGG 4 cut(s) 177, 619, 882, 974
MvnI CGCG 1 cut(s) 442
MwoI GCNNNNNNNGC 3 cut(s) 191, 217, 764
NciI CCSGG 1 cut(s) 60
NcoI CCATGG 1 cut(s) 464
NdeII GATC 4 cut(s) 102, 179, 213, 946
NlaIII CATG 4 cut(s) 220, 232, 468, 545
NlaIV GGNNCC 5 cut(s) 83, 471, 488, 538, 861
NmuCI GTSAC 1 cut(s) 801
NspV TTCGAA 1 cut(s) 890
OliI CACNNNNGTG 1 cut(s) 1077
PfeI GAWTC 7 cut(s) 274, 289, 560, 671, 712, 1025, 1102
PflFI GACNNNGTC 1 cut(s) 1140
PfoI TCCNGGA 1 cut(s) 175
PkrI GCNGC 2 cut(s) 57, 837
PleI GAGTC 1 cut(s) 717
PpsI GAGTC 1 cut(s) 717
PpuMI RGGWCCY 2 cut(s) 81, 607
PshBI ATTAAT 1 cut(s) 480
Psp5II RGGWCCY 2 cut(s) 81, 607
Psp6I CCWGG 4 cut(s) 175, 617, 880, 972
PspGI CCWGG 4 cut(s) 175, 617, 880, 972
PspN4I GGNNCC 5 cut(s) 83, 471, 488, 538, 861
PspPI GGNCC 6 cut(s) 81, 143, 486, 536, 607, 653
PspPPI RGGWCCY 2 cut(s) 81, 607
PsrI GAACNNNNNNTAC 2 cut(s) 984, 1016
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 2 cut(s) 102, 946
PsyI GACNNNGTC 1 cut(s) 1140
PvuII CAGCTG 2 cut(s) 194, 835
RsaI GTAC 3 cut(s) 156, 349, 1193
RsaNI GTAC 3 cut(s) 155, 348, 1192
RseI CAYNNNNRTG 2 cut(s) 342, 1077
SalI GTCGAC 1 cut(s) 566
SaqAI TTAA 5 cut(s) 480, 623, 629, 753, 819
SatI GCNGC 2 cut(s) 56, 836
Sau3AI GATC 4 cut(s) 102, 179, 213, 946
Sau96I GGNCC 6 cut(s) 81, 143, 486, 536, 607, 653
SchI GAGTC 1 cut(s) 717
ScrFI CCNGG 5 cut(s) 60, 177, 619, 882, 974
SduI GDGCHC 1 cut(s) 472
SfaNI GCATC 5 cut(s) 304, 397, 582, 847, 1193
SfcI CTRYAG 2 cut(s) 453, 703
SfuI TTCGAA 1 cut(s) 890
SinI GGWCC 4 cut(s) 81, 486, 607, 653
SmiMI CAYNNNNRTG 2 cut(s) 342, 1077
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
Sse9I AATT 6 cut(s) 108, 305, 481, 770, 1064, 1124
SsiI CCGC 5 cut(s) 56, 633, 656, 708, 903
SspMI CTAG 2 cut(s) 293, 915
StyD4I CCNGG 5 cut(s) 58, 175, 617, 880, 972
StyI CCWWGG 2 cut(s) 355, 464
TaaI ACNGT 2 cut(s) 643, 1208
TaqI TCGA 4 cut(s) 531, 567, 865, 890
TaqII GACCGA 1 cut(s) 396
TasI AATT 6 cut(s) 108, 305, 481, 770, 1064, 1124
TatI WGTACW 1 cut(s) 1191
TauI GCSGC 1 cut(s) 58
TfiI GAWTC 7 cut(s) 274, 289, 560, 671, 712, 1025, 1102
Tru1I TTAA 5 cut(s) 480, 623, 629, 753, 819
Tru9I TTAA 5 cut(s) 480, 623, 629, 753, 819
TscAI CASTG 2 cut(s) 128, 1126
TseFI GTSAC 1 cut(s) 801
TseI GCWGC 1 cut(s) 835
Tsp45I GTSAC 1 cut(s) 801
TspDTI ATGAA 8 cut(s) 53, 245, 663, 667, 747, 828, 1151, 1161
TspGWI ACGGA 1 cut(s) 783
TspRI CASTG 2 cut(s) 128, 1126
Tth111I GACNNNGTC 1 cut(s) 1140
VpaK11BI GGWCC 4 cut(s) 81, 486, 607, 653
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 3 cut(s) 305, 1064, 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 1079
XmiI GTMKAC 2 cut(s) 48, 567
XspI CTAG 2 cut(s) 293, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.