pycom06g06530

Ycf49-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008707
Physical Location & Seq
Reverse (-)
10387520 .. 10390193
2674 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGTATTTTCATCTTCACACAAATCGAGAACCAATCCAAATCATTCCCATTATACCCTCCGTCTCCAACCCTCCGTCTCCAACCCTGCTGCTGCTTCAAAACATCAGTGGGCAATTCTTGAGACAGAAAAGCCCAACGGCCATGGCAATGCACTCCTTACTTCACCTTCCAAAACCCACTTTCCCGCGCAAACCCCTTTTCCCTCCGCGCCCAAAACCTCAAACCCAACCGCCAAATTCCCACAAAAACTTGGTGGGTCTACTCGGGGCGGGTCTGACACTGGCCTTCGTCGGACCCGCCTCAGCTTCTGAGCTGCTGCCCTTGAATGAACCCTCCAATGCCCTCTCCTTGCCCACCTGGGCCATTCACGTTTCCAGCGTCGTTGAATGGGTTACAGCAATGGCATTGGTGTGGCAGTACGGGGAGAAGTCCGGGTTCGAATCTTGGAAAGGGCTGTCTTGGGGGATGGTTCCCTTGCTTGGTGGAGCATTATGTGCATGCACTTGGCATTTCTTTTATAACTCTGAGTCTCTTGAAGTACTGGTGGCTCTTCAAGCAGCCCTGACAGTAATAGGCAATGCCACAATGTGTTTTGCTGCATATCGTATTTACAGATCATCCGAAGAACGCTCCAAGAATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2499

Protein Analysis

215

Amino Acids

23.55

Weight (kDa)

9.27

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2499 PF10693 114 - 201 3.9e-39 Protein of unknown function (DUF2499)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
AccI GTMKAC 1 cut(s) 259
AccII CGCG 2 cut(s) 187, 208
AciI CCGC 5 cut(s) 185, 206, 230, 269, 297
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 1 cut(s) 235
AdeI CACNNNGTG 1 cut(s) 588
AfaI GTAC 2 cut(s) 419, 540
AfiI CCNNNNNNNGG 1 cut(s) 479
AgsI TTSAA 5 cut(s) 98, 325, 386, 536, 554
AjnI CCWGG 1 cut(s) 356
AluBI AGCT 2 cut(s) 305, 313
AluI AGCT 2 cut(s) 305, 313
Alw26I GTCTC 4 cut(s) 67, 81, 115, 534
AlwNI CAGNNNCTG 1 cut(s) 308
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 3 cut(s) 138, 282, 360
ApeKI GCWGC 6 cut(s) 88, 91, 313, 316, 557, 596
ApoI RAATTY 1 cut(s) 235
AspLEI GCGC 2 cut(s) 189, 210
AspS9I GGNCC 2 cut(s) 293, 360
AsuC2I CCSGG 1 cut(s) 433
AsuHPI GGTGA 1 cut(s) 155
AsuII TTCGAA 1 cut(s) 438
AvaI CYCGRG 1 cut(s) 263
AvaII GGWCC 1 cut(s) 293
BbvCI CCTCAGC 1 cut(s) 301
BbvI GCAGC 6 cut(s) 75, 78, 300, 303, 569, 583
BccI CCATC 1 cut(s) 460
BceAI ACGGC 1 cut(s) 153
BciT130I CCWGG 1 cut(s) 358
BcnI CCSGG 1 cut(s) 433
BcoDI GTCTC 4 cut(s) 67, 81, 115, 534
BisI GCNGC 6 cut(s) 89, 92, 314, 317, 558, 597
BlsI GCNGC 6 cut(s) 90, 93, 315, 318, 559, 598
BmcAI AGTACT 1 cut(s) 540
Bme1390I CCNGG 2 cut(s) 358, 433
Bme18I GGWCC 1 cut(s) 293
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 2 cut(s) 293, 360
BmiI GGNNCC 2 cut(s) 295, 471
BmrFI CCNGG 2 cut(s) 358, 433
Bpu10I CCTNAGC 1 cut(s) 301
Bpu14I TTCGAA 1 cut(s) 438
BpuEI CTTGAG 1 cut(s) 139
BpuMI CCSGG 1 cut(s) 433
BsaJI CCNNGG 2 cut(s) 141, 357
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse1I ACTGG 2 cut(s) 285, 546
Bse3DI GCAATG 3 cut(s) 153, 405, 583
BseBI CCWGG 1 cut(s) 358
BseDI CCNNGG 2 cut(s) 141, 357
BseGI GGATG 2 cut(s) 471, 617
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMI GCAATG 3 cut(s) 153, 405, 583
BseMII CTCAG 3 cut(s) 300, 315, 516
BseNI ACTGG 2 cut(s) 285, 546
BseXI GCAGC 6 cut(s) 75, 78, 300, 303, 569, 583
Bsh1236I CGCG 2 cut(s) 187, 208
BshFI GGCC 3 cut(s) 140, 284, 362
BsiHKCI CYCGRG 1 cut(s) 263
BsiSI CCGG 1 cut(s) 432
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 4 cut(s) 67, 81, 115, 534
BsmBI CGTCTC 2 cut(s) 67, 81
BsnI GGCC 3 cut(s) 140, 284, 362
BsoBI CYCGRG 1 cut(s) 263
Bsp119I TTCGAA 1 cut(s) 438
Bsp143I GATC 1 cut(s) 614
Bsp19I CCATGG 1 cut(s) 141
BspACI CCGC 5 cut(s) 185, 206, 230, 269, 297
BspANI GGCC 3 cut(s) 140, 284, 362
BspCNI CTCAG 3 cut(s) 301, 314, 517
BspFNI CGCG 2 cut(s) 187, 208
BspLI GGNNCC 2 cut(s) 295, 471
BspQI GCTCTTC 1 cut(s) 555
BspT104I TTCGAA 1 cut(s) 438
BsrDI GCAATG 3 cut(s) 153, 405, 583
BsrI ACTGG 2 cut(s) 285, 546
BssECI CCNNGG 2 cut(s) 141, 357
BssMI GATC 1 cut(s) 614
BssT1I CCWWGG 1 cut(s) 141
Bst2UI CCWGG 1 cut(s) 358
Bst4CI ACNGT 1 cut(s) 568
Bst6I CTCTTC 1 cut(s) 555
BstAPI GCANNNNNTGC 1 cut(s) 494
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 301, 309, 525
BstDSI CCRYGG 1 cut(s) 141
BstF5I GGATG 2 cut(s) 471, 617
BstFNI CGCG 2 cut(s) 187, 208
BstHHI GCGC 2 cut(s) 189, 210
BstKTI GATC 1 cut(s) 617
BstMAI GTCTC 4 cut(s) 67, 81, 115, 534
BstMBI GATC 1 cut(s) 614
BstMWI GCNNNNNNNGC 2 cut(s) 494, 554
BstNI CCWGG 1 cut(s) 358
BstNSI RCATGY 1 cut(s) 501
BstSCI CCNGG 2 cut(s) 356, 431
BstUI CGCG 2 cut(s) 187, 208
BstV1I GCAGC 6 cut(s) 75, 78, 300, 303, 569, 583
BsuRI GGCC 3 cut(s) 140, 284, 362
BtgI CCRYGG 1 cut(s) 141
BtsCI GGATG 2 cut(s) 471, 617
BtsIMutI CAGTG 2 cut(s) 112, 278
Cac8I GCNNGC 1 cut(s) 499
CaiI CAGNNNCTG 1 cut(s) 308
CfoI GCGC 2 cut(s) 189, 210
Cfr13I GGNCC 2 cut(s) 293, 360
CseI GACGC 1 cut(s) 367
Csp6I GTAC 2 cut(s) 418, 539
CviAII CATG 2 cut(s) 142, 498
CviJI RGCY 9 cut(s) 132, 140, 284, 305, 313, 362, 454, 548, 560
CviKI_1 RGCY 9 cut(s) 132, 140, 284, 305, 313, 362, 454, 548, 560
CviQI GTAC 2 cut(s) 418, 539
DdeI CTNAG 3 cut(s) 301, 309, 525
DpnI GATC 1 cut(s) 616
DpnII GATC 1 cut(s) 614
DraIII CACNNNGTG 1 cut(s) 588
EaeI YGGCCR 1 cut(s) 138
Eam1104I CTCTTC 1 cut(s) 555
EarI CTCTTC 1 cut(s) 555
Eco130I CCWWGG 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 293
Eco88I CYCGRG 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 141
ErhI CCWWGG 1 cut(s) 141
Esp3I CGTCTC 2 cut(s) 67, 81
FaeI CATG 2 cut(s) 145, 501
FaiI YATR 6 cut(s) 53, 143, 493, 499, 519, 601
FatI CATG 2 cut(s) 141, 497
FauI CCCGC 3 cut(s) 192, 262, 304
FblI GTMKAC 1 cut(s) 259
Fnu4HI GCNGC 6 cut(s) 89, 92, 314, 317, 558, 597
FokI GGATG 2 cut(s) 478, 604
Fsp4HI GCNGC 6 cut(s) 89, 92, 314, 317, 558, 597
GlaI GCGC 2 cut(s) 188, 209
GluI GCNGC 6 cut(s) 89, 92, 314, 317, 558, 597
HaeIII GGCC 3 cut(s) 140, 284, 362
HapII CCGG 1 cut(s) 432
HgaI GACGC 1 cut(s) 367
HhaI GCGC 2 cut(s) 189, 210
Hin1II CATG 2 cut(s) 145, 501
Hin6I GCGC 2 cut(s) 187, 208
HinP1I GCGC 2 cut(s) 187, 208
HinfI GANTC 3 cut(s) 440, 527, 637
HpaII CCGG 1 cut(s) 432
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 260
Hpy188I TCNGA 5 cut(s) 276, 293, 310, 526, 622
Hpy188III TCNNGA 3 cut(s) 26, 118, 533
Hpy8I GTNNAC 1 cut(s) 260
Hpy99I CGWCG 2 cut(s) 293, 383
HpyAV CCTTC 2 cut(s) 176, 295
HpyCH4III ACNGT 1 cut(s) 568
HpyCH4IV ACGT 1 cut(s) 369
HpyCH4V TGCA 4 cut(s) 151, 497, 501, 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 554
HpyF3I CTNAG 3 cut(s) 301, 309, 525
HpySE526I ACGT 1 cut(s) 369
Hsp92II CATG 2 cut(s) 145, 501
HspAI GCGC 2 cut(s) 187, 208
Kzo9I GATC 1 cut(s) 614
LguI GCTCTTC 1 cut(s) 555
LmnI GCTCC 2 cut(s) 485, 635
LpnPI CCDG 8 cut(s) 98, 266, 343, 370, 388, 445, 527, 575
Lsp1109I GCAGC 6 cut(s) 75, 78, 300, 303, 569, 583
MaeII ACGT 1 cut(s) 369
MaeIII GTNAC 1 cut(s) 391
MalI GATC 1 cut(s) 616
MboI GATC 1 cut(s) 614
MboII GAAGA 3 cut(s) 5, 542, 635
MluCI AATT 2 cut(s) 113, 235
MlyI GAGTC 1 cut(s) 536
MmeI TCCRAC 3 cut(s) 90, 104, 271
MnlI CCTC 7 cut(s) 67, 81, 213, 228, 310, 343, 353
MslI CAYNNNNRTG 2 cut(s) 146, 409
MspI CCGG 1 cut(s) 432
MspR9I CCNGG 2 cut(s) 358, 433
MvaI CCWGG 1 cut(s) 358
MvnI CGCG 2 cut(s) 187, 208
MwoI GCNNNNNNNGC 2 cut(s) 494, 554
NciI CCSGG 1 cut(s) 433
NcoI CCATGG 1 cut(s) 141
NdeII GATC 1 cut(s) 614
NlaIII CATG 2 cut(s) 145, 501
NlaIV GGNNCC 2 cut(s) 295, 471
NspI RCATGY 1 cut(s) 501
NspV TTCGAA 1 cut(s) 438
PaeI GCATGC 1 cut(s) 501
PciSI GCTCTTC 1 cut(s) 555
PcsI WCGNNNNNNNCGW 1 cut(s) 375
PfeI GAWTC 2 cut(s) 440, 637
PkrI GCNGC 6 cut(s) 90, 93, 315, 318, 559, 598
PleI GAGTC 1 cut(s) 535
PpsI GAGTC 1 cut(s) 535
PsiI TTATAA 1 cut(s) 519
Psp6I CCWGG 1 cut(s) 356
PspGI CCWGG 1 cut(s) 356
PspN4I GGNNCC 2 cut(s) 295, 471
PspPI GGNCC 2 cut(s) 293, 360
PstNI CAGNNNCTG 1 cut(s) 308
RsaI GTAC 2 cut(s) 419, 540
RsaNI GTAC 2 cut(s) 418, 539
RseI CAYNNNNRTG 2 cut(s) 146, 409
SapI GCTCTTC 1 cut(s) 555
SatI GCNGC 6 cut(s) 89, 92, 314, 317, 558, 597
Sau3AI GATC 1 cut(s) 614
Sau96I GGNCC 2 cut(s) 293, 360
ScaI AGTACT 1 cut(s) 540
SchI GAGTC 1 cut(s) 536
ScrFI CCNGG 2 cut(s) 358, 433
SetI ASST 6 cut(s) 168, 220, 307, 315, 359, 372
SfuI TTCGAA 1 cut(s) 438
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 2 cut(s) 146, 409
SmlI CTYRAG 1 cut(s) 118
SmoI CTYRAG 1 cut(s) 118
SphI GCATGC 1 cut(s) 501
Sse9I AATT 2 cut(s) 113, 235
SsiI CCGC 5 cut(s) 185, 206, 230, 269, 297
StyD4I CCNGG 2 cut(s) 356, 431
StyI CCWWGG 1 cut(s) 141
TaaI ACNGT 1 cut(s) 568
TaiI ACGT 1 cut(s) 372
TaqI TCGA 2 cut(s) 25, 438
TasI AATT 2 cut(s) 113, 235
TatI WGTACW 1 cut(s) 538
TfiI GAWTC 2 cut(s) 440, 637
TscAI CASTG 2 cut(s) 112, 285
TseI GCWGC 6 cut(s) 88, 91, 313, 316, 557, 596
TspDTI ATGAA 1 cut(s) 342
TspGWI ACGGA 2 cut(s) 49, 63
TspRI CASTG 2 cut(s) 112, 285
VpaK11BI GGWCC 1 cut(s) 293
XapI RAATTY 1 cut(s) 235
XceI RCATGY 1 cut(s) 501
XmiI GTMKAC 1 cut(s) 259
ZrmI AGTACT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.