pycom06g10060

Mitochondrial inner membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009287
Physical Location & Seq
Reverse (-)
15405061 .. 15408809
3749 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1314 bp
ATGGCTTACCGGCGGAGCCTCACGACGACGAGCGGTATAGCTCGACGGCACCAGTACCATCCGTCCGTCACTCACATCCTCCGCGACGATGACCGCAAGCAAGATGTCGTTTCCGAGCACGCATCTGCTTCGCCGATATTGAATCAATTTCTCAACCAGAAGCGGTTTTTCGGAAACAATTTGAGCAGAGTGTCGGGTTTCGGCGGTGGGTTCGCTCAGGATAGGAGGATTTCGAATGCTCTGCTTTCGTCCACTTCGGGATTCGCGTTCTCTCGATCCATGTCGTCGGCTTCGGAGAAGGTTGAGCTGTTGAGTGATGTATCGGGAGTTTTATCCGACACTGGGATCGAAGGCTTGACTTCTCAGGTACCGGCAATGAGCGAGGTGGCGGTTGCTGCCGCGGATTCGTGGTTGCCAGTTAAGGGCTTGCAGTACTTTATTGAAGCTGTGCATGTTCACACTGGCTTGAATTGGTGGGCCGCCATAGTGATAACAACTCTTTTGATTCGGACTGCCACGGTTCCGCTTTTGATTAATCAACTTAAAGCAACCTCAAAACTCTCTCTCATGAAACCTCAACTTGAAGAATTGAAGCAAGAGATGCAAGACAGGGGTTCAGATCGTTCTGCTCAGCTTGAATTTCAACAGAAAACAAAGAAGCTATTTAAGGAATATGGTGTAACCCCATTGACTCCAATGAAGGGACTTTTGATTCAAGGTCCTGTATTCATCAGCTTTTTCCTTGCTATAGGTAACATGGCAGAGAAAGTGCCATCCTTTAAAACTGGCGGCGCCTACTGGTTTCTTGATCTTACAACCCCAGATGCCATGTACGTTTTTCCAGTTTTGGCAGCCATGACATTCTTACTGACTGTGGAGTGCAATCTGCAAGAAGGTATGGAGGGAAATCCTGCTGCTGGCACCATGAAAAATGTTTCAAGAGGCCTTGCTGTTCTTACAGTTCCATTTACCATGAGCTTTCCAAAGGCTGTATTTTGTTACTGGGTTACATCCAACTTGTTTTCGCTCTCGTATGGACTGGTGCTTAAATATACTGGGCTGAAGGAGGCCTTGGGTATTCCTAAACCGCCGCCTTATACTGCACCCCCTCAAGGATCACAACCTGCATTTTCACTATTATCCACACTGAAACAAGTCGCTGAAGGGGCTCGTGGCTCAACCTCCTCACCTGGTGACGCATCAAAGGTTCAAGATCGAAGTATATCATCTTCGTCGTCATCATCAGTTCTTAGTCAAAGGATTAAAAGTTTAGAGAAGCAAGTGAAGGGAAGAAAGAGAAACAAGAAGAGGTAA

Protein Analysis

438

Amino Acids

47.71

Weight (kDa)

9.77

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
60KD_IMP PF02096 158 - 346 8.5e-29 60Kd inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1132
Acc65I GGTACC 1 cut(s) 367
AccB1I GGYRCC 4 cut(s) 48, 367, 791, 920
AccBSI CCGCTC 1 cut(s) 33
AccII CGCG 3 cut(s) 84, 266, 401
AclWI GGATC 3 cut(s) 270, 353, 1123
AcsI RAATTY 1 cut(s) 638
AcuI CTGAAG 2 cut(s) 1082, 1182
AcyI GRCGYC 1 cut(s) 792
AdeI CACNNNGTG 1 cut(s) 1193
AfaI GTAC 4 cut(s) 56, 369, 434, 833
AfiI CCNNNNNNNGG 5 cut(s) 342, 422, 701, 917, 1112
AjnI CCWGG 1 cut(s) 1189
AjuI GAANNNNNNNTTGG 2 cut(s) 1055, 1087
AluBI AGCT 7 cut(s) 41, 307, 446, 634, 661, 735, 978
AluI AGCT 7 cut(s) 41, 307, 446, 634, 661, 735, 978
Alw21I GWGCWC 1 cut(s) 120
AlwI GGATC 3 cut(s) 270, 353, 1123
AoxI GGCC 3 cut(s) 477, 943, 1068
ApeKI GCWGC 3 cut(s) 395, 851, 914
ApoI RAATTY 1 cut(s) 638
AseI ATTAAT 1 cut(s) 534
Asp718I GGTACC 1 cut(s) 367
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 2 cut(s) 477, 719
AsuHPI GGTGA 2 cut(s) 1179, 1205
AsuII TTCGAA 1 cut(s) 233
AvaII GGWCC 1 cut(s) 719
BanI GGYRCC 4 cut(s) 48, 367, 791, 920
BanII GRGCYC 1 cut(s) 1171
BarI GAAGNNNNNNTAC 2 cut(s) 1213, 1245
BauI CACGAG 1 cut(s) 1170
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 3 cut(s) 382, 863, 901
BccI CCATC 2 cut(s) 66, 781
BceAI ACGGC 1 cut(s) 62
BcgI CGANNNNNNTGC 4 cut(s) 111, 145, 223, 257
BciT130I CCWGG 1 cut(s) 1191
BfmI CTRYAG 1 cut(s) 747
BfoI RGCGCY 1 cut(s) 795
BfuAI ACCTGC 1 cut(s) 1132
BisI GCNGC 7 cut(s) 396, 399, 480, 790, 852, 915, 1091
BlpI GCTNAGC 1 cut(s) 630
BlsI GCNGC 7 cut(s) 397, 400, 481, 791, 853, 916, 1092
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 1 cut(s) 1191
Bme18I GGWCC 1 cut(s) 719
BmgT120I GGNCC 2 cut(s) 477, 719
BmiI GGNNCC 6 cut(s) 17, 50, 369, 522, 793, 922
BmrFI CCNGG 1 cut(s) 1191
BmrI ACTGGG 3 cut(s) 351, 1012, 1065
BmsI GCATC 4 cut(s) 131, 591, 814, 1208
BmuI ACTGGG 3 cut(s) 351, 1012, 1065
Bpu10I CCTNAGC 1 cut(s) 216
Bpu1102I GCTNAGC 1 cut(s) 630
Bpu14I TTCGAA 1 cut(s) 233
BpuEI CTTGAG 1 cut(s) 1095
BsaHI GRCGYC 1 cut(s) 792
BsaJI CCNNGG 3 cut(s) 399, 516, 1071
Bsc4I CCNNNNNNNGG 5 cut(s) 342, 422, 701, 917, 1112
Bse118I RCCGGY 2 cut(s) 9, 370
Bse3DI GCAATG 1 cut(s) 381
BseBI CCWGG 1 cut(s) 1191
BseDI CCNNGG 3 cut(s) 399, 516, 1071
BseGI GGATG 4 cut(s) 58, 75, 773, 1010
BseLI CCNNNNNNNGG 5 cut(s) 342, 422, 701, 917, 1112
BseMI GCAATG 1 cut(s) 381
BseMII CTCAG 3 cut(s) 230, 377, 644
BseRI GAGGAG 1 cut(s) 1174
BseXI GCAGC 3 cut(s) 382, 863, 901
BsgI GTGCAG 1 cut(s) 1086
Bsh1236I CGCG 3 cut(s) 84, 266, 401
BshFI GGCC 3 cut(s) 479, 945, 1070
BshNI GGYRCC 4 cut(s) 48, 367, 791, 920
BsiHKAI GWGCWC 1 cut(s) 120
BsiSI CCGG 2 cut(s) 10, 371
BslFI GGGAC 1 cut(s) 717
BslI CCNNNNNNNGG 5 cut(s) 342, 422, 701, 917, 1112
BsmFI GGGAC 1 cut(s) 717
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 3 cut(s) 479, 945, 1070
Bsp119I TTCGAA 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 120, 1171
Bsp143I GATC 6 cut(s) 275, 345, 619, 808, 1115, 1213
Bsp1720I GCTNAGC 1 cut(s) 630
BspANI GGCC 3 cut(s) 479, 945, 1070
BspCNI CTCAG 3 cut(s) 229, 376, 643
BspFNI CGCG 3 cut(s) 84, 266, 401
BspHI TCATGA 1 cut(s) 567
BspLI GGNNCC 6 cut(s) 17, 50, 369, 522, 793, 922
BspMI ACCTGC 1 cut(s) 1132
BspPI GGATC 3 cut(s) 270, 353, 1123
BspT104I TTCGAA 1 cut(s) 233
BspT107I GGYRCC 4 cut(s) 48, 367, 791, 920
BsrBI CCGCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 381
BsrFI RCCGGY 2 cut(s) 9, 370
BssAI RCCGGY 2 cut(s) 9, 370
BssECI CCNNGG 3 cut(s) 399, 516, 1071
BssMI GATC 6 cut(s) 275, 345, 619, 808, 1115, 1213
BssNI GRCGYC 1 cut(s) 792
BssSI CACGAG 1 cut(s) 1170
BssT1I CCWWGG 1 cut(s) 1071
Bst2BI CACGAG 1 cut(s) 1170
Bst2UI CCWGG 1 cut(s) 1191
Bst4CI ACNGT 3 cut(s) 520, 874, 961
Bst6I CTCTTC 1 cut(s) 1301
BstACI GRCGYC 1 cut(s) 792
BstAPI GCANNNNNTGC 1 cut(s) 601
BstBI TTCGAA 1 cut(s) 233
BstC8I GCNNGC 4 cut(s) 98, 120, 428, 919
BstDEI CTNAG 4 cut(s) 216, 363, 630, 1250
BstDSI CCRYGG 2 cut(s) 399, 516
BstF5I GGATG 4 cut(s) 58, 75, 773, 1010
BstFNI CGCG 3 cut(s) 84, 266, 401
BstH2I RGCGCY 1 cut(s) 795
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 6 cut(s) 278, 348, 622, 811, 1118, 1216
BstMBI GATC 6 cut(s) 275, 345, 619, 808, 1115, 1213
BstMWI GCNNNNNNNGC 3 cut(s) 395, 601, 1166
BstNI CCWGG 1 cut(s) 1191
BstNSI RCATGY 1 cut(s) 455
BstSCI CCNGG 1 cut(s) 1189
BstSFI CTRYAG 1 cut(s) 747
BstUI CGCG 3 cut(s) 84, 266, 401
BstV1I GCAGC 3 cut(s) 382, 863, 901
BsuRI GGCC 3 cut(s) 479, 945, 1070
BtgI CCRYGG 2 cut(s) 399, 516
BtsCI GGATG 4 cut(s) 58, 75, 773, 1010
BtsIMutI CAGTG 3 cut(s) 339, 459, 1145
BveI ACCTGC 1 cut(s) 1132
Cac8I GCNNGC 4 cut(s) 98, 120, 428, 919
CciI TCATGA 1 cut(s) 567
CfoI GCGC 1 cut(s) 794
Cfr10I RCCGGY 2 cut(s) 9, 370
Cfr13I GGNCC 2 cut(s) 477, 719
Cfr42I CCGCGG 1 cut(s) 402
CseI GACGC 1 cut(s) 1205
CsiI ACCWGGT 1 cut(s) 1189
Csp6I GTAC 4 cut(s) 55, 368, 433, 832
CviAII CATG 8 cut(s) 280, 452, 568, 757, 829, 856, 925, 973
CviQI GTAC 4 cut(s) 55, 368, 433, 832
DdeI CTNAG 4 cut(s) 216, 363, 630, 1250
DinI GGCGCC 1 cut(s) 793
DpnI GATC 6 cut(s) 277, 347, 621, 810, 1117, 1215
DpnII GATC 6 cut(s) 275, 345, 619, 808, 1115, 1213
DraI TTTAAA 1 cut(s) 781
DraIII CACNNNGTG 1 cut(s) 1193
Eam1104I CTCTTC 1 cut(s) 1301
EarI CTCTTC 1 cut(s) 1301
EciI GGCGGA 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 1071
Eco147I AGGCCT 2 cut(s) 945, 1070
Eco24I GRGCYC 1 cut(s) 1171
Eco47I GGWCC 1 cut(s) 719
Eco57I CTGAAG 2 cut(s) 1082, 1182
EcoO109I RGGNCCY 1 cut(s) 719
EcoRII CCWGG 1 cut(s) 1189
EcoT14I CCWWGG 1 cut(s) 1071
EcoT38I GRGCYC 1 cut(s) 1171
EgeI GGCGCC 1 cut(s) 793
EheI GGCGCC 1 cut(s) 793
ErhI CCWWGG 1 cut(s) 1071
FaeI CATG 8 cut(s) 283, 455, 571, 760, 832, 859, 928, 976
FalI AAGNNNNNCTT 2 cut(s) 1055, 1087
FaqI GGGAC 1 cut(s) 717
FatI CATG 8 cut(s) 279, 451, 567, 756, 828, 855, 924, 972
Fnu4HI GCNGC 7 cut(s) 396, 399, 480, 790, 852, 915, 1091
FokI GGATG 4 cut(s) 45, 62, 760, 997
FriOI GRGCYC 1 cut(s) 1171
Fsp4HI GCNGC 7 cut(s) 396, 399, 480, 790, 852, 915, 1091
GlaI GCGC 1 cut(s) 793
GluI GCNGC 7 cut(s) 396, 399, 480, 790, 852, 915, 1091
HaeII RGCGCY 1 cut(s) 795
HaeIII GGCC 3 cut(s) 479, 945, 1070
HapII CCGG 2 cut(s) 10, 371
HgaI GACGC 1 cut(s) 1205
HhaI GCGC 1 cut(s) 794
Hin1I GRCGYC 1 cut(s) 792
Hin1II CATG 8 cut(s) 283, 455, 571, 760, 832, 859, 928, 976
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HinfI GANTC 6 cut(s) 142, 261, 404, 505, 691, 712
HpaII CCGG 2 cut(s) 10, 371
HphI GGTGA 2 cut(s) 1179, 1205
Hpy166II GTNNAC 2 cut(s) 252, 457
Hpy188I TCNGA 6 cut(s) 115, 173, 295, 337, 510, 619
Hpy188III TCNNGA 9 cut(s) 22, 218, 258, 273, 324, 568, 806, 939, 1211
Hpy8I GTNNAC 2 cut(s) 252, 457
Hpy99I CGWCG 6 cut(s) 28, 31, 48, 89, 289, 1237
HpyAV CCTTC 7 cut(s) 292, 344, 694, 887, 1057, 1157, 1279
HpyCH4III ACNGT 3 cut(s) 520, 874, 961
HpyCH4IV ACGT 1 cut(s) 834
HpyCH4V TGCA 7 cut(s) 430, 451, 604, 882, 889, 1103, 1127
HpyF10VI GCNNNNNNNGC 3 cut(s) 395, 601, 1166
HpyF3I CTNAG 4 cut(s) 216, 363, 630, 1250
HpySE526I ACGT 1 cut(s) 834
Hsp92I GRCGYC 1 cut(s) 792
Hsp92II CATG 8 cut(s) 283, 455, 571, 760, 832, 859, 928, 976
HspAI GCGC 1 cut(s) 792
KasI GGCGCC 1 cut(s) 791
KpnI GGTACC 1 cut(s) 371
KspI CCGCGG 1 cut(s) 402
Kzo9I GATC 6 cut(s) 275, 345, 619, 808, 1115, 1213
LmnI GCTCC 1 cut(s) 15
Lsp1109I GCAGC 3 cut(s) 382, 863, 901
LweI GCATC 4 cut(s) 131, 591, 814, 1208
MabI ACCWGGT 1 cut(s) 1189
MaeII ACGT 1 cut(s) 834
MaeIII GTNAC 6 cut(s) 67, 679, 752, 998, 1006, 1193
MalI GATC 6 cut(s) 277, 347, 621, 810, 1117, 1215
MbiI CCGCTC 1 cut(s) 33
MboI GATC 6 cut(s) 275, 345, 619, 808, 1115, 1213
MboII GAAGA 3 cut(s) 596, 1221, 1302
MhlI GDGCHC 2 cut(s) 120, 1171
MluCI AATT 5 cut(s) 146, 178, 469, 587, 638
Mly113I GGCGCC 1 cut(s) 792
MlyI GAGTC 1 cut(s) 685
MmeI TCCRAC 2 cut(s) 360, 1038
MseI TTAA 7 cut(s) 420, 534, 543, 666, 780, 1047, 1263
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 2 cut(s) 10, 371
MspR9I CCNGG 1 cut(s) 1191
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 1 cut(s) 1191
MvnI CGCG 3 cut(s) 84, 266, 401
MwoI GCNNNNNNNGC 3 cut(s) 395, 601, 1166
NarI GGCGCC 1 cut(s) 792
NdeII GATC 6 cut(s) 275, 345, 619, 808, 1115, 1213
NlaIII CATG 8 cut(s) 283, 455, 571, 760, 832, 859, 928, 976
NlaIV GGNNCC 6 cut(s) 17, 50, 369, 522, 793, 922
NmuCI GTSAC 2 cut(s) 67, 1193
NspI RCATGY 1 cut(s) 455
NspV TTCGAA 1 cut(s) 233
PagI TCATGA 1 cut(s) 567
PceI AGGCCT 2 cut(s) 945, 1070
PcsI WCGNNNNNNNCGW 1 cut(s) 263
PctI GAATGC 1 cut(s) 241
PfeI GAWTC 5 cut(s) 142, 261, 404, 505, 712
PkrI GCNGC 7 cut(s) 397, 400, 481, 791, 853, 916, 1092
PleI GAGTC 1 cut(s) 685
PluTI GGCGCC 1 cut(s) 795
PpsI GAGTC 1 cut(s) 685
PpuMI RGGWCCY 1 cut(s) 719
PshBI ATTAAT 1 cut(s) 534
Psp5II RGGWCCY 1 cut(s) 719
Psp6I CCWGG 1 cut(s) 1189
PspGI CCWGG 1 cut(s) 1189
PspN4I GGNNCC 6 cut(s) 17, 50, 369, 522, 793, 922
PspPI GGNCC 2 cut(s) 477, 719
PspPPI RGGWCCY 1 cut(s) 719
RsaI GTAC 4 cut(s) 56, 369, 434, 833
RsaNI GTAC 4 cut(s) 55, 368, 433, 832
SacII CCGCGG 1 cut(s) 402
SaqAI TTAA 7 cut(s) 420, 534, 543, 666, 780, 1047, 1263
SatI GCNGC 7 cut(s) 396, 399, 480, 790, 852, 915, 1091
Sau3AI GATC 6 cut(s) 275, 345, 619, 808, 1115, 1213
Sau96I GGNCC 2 cut(s) 477, 719
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 1 cut(s) 685
ScrFI CCNGG 1 cut(s) 1191
SduI GDGCHC 2 cut(s) 120, 1171
SexAI ACCWGGT 1 cut(s) 1189
SfaNI GCATC 4 cut(s) 131, 591, 814, 1208
SfcI CTRYAG 1 cut(s) 747
SfoI GGCGCC 1 cut(s) 793
Sfr303I CCGCGG 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 233
SgrBI CCGCGG 1 cut(s) 402
SinI GGWCC 1 cut(s) 719
SmlI CTYRAG 1 cut(s) 1110
SmoI CTYRAG 1 cut(s) 1110
Sse9I AATT 5 cut(s) 146, 178, 469, 587, 638
SseBI AGGCCT 2 cut(s) 945, 1070
SspDI GGCGCC 1 cut(s) 791
StuI AGGCCT 2 cut(s) 945, 1070
StyD4I CCNGG 1 cut(s) 1189
StyI CCWWGG 1 cut(s) 1071
TaaI ACNGT 3 cut(s) 520, 874, 961
TaiI ACGT 1 cut(s) 837
TaqI TCGA 5 cut(s) 43, 233, 274, 348, 1216
TasI AATT 5 cut(s) 146, 178, 469, 587, 638
TatI WGTACW 1 cut(s) 432
TauI GCSGC 4 cut(s) 401, 482, 792, 1093
TfiI GAWTC 5 cut(s) 142, 261, 404, 505, 712
Tru1I TTAA 7 cut(s) 420, 534, 543, 666, 780, 1047, 1263
Tru9I TTAA 7 cut(s) 420, 534, 543, 666, 780, 1047, 1263
TscAI CASTG 3 cut(s) 346, 466, 1152
TseFI GTSAC 2 cut(s) 67, 1193
TseI GCWGC 3 cut(s) 395, 851, 914
Tsp45I GTSAC 2 cut(s) 67, 1193
TspDTI ATGAA 4 cut(s) 584, 713, 718, 941
TspGWI ACGGA 2 cut(s) 51, 55
TspRI CASTG 3 cut(s) 346, 466, 1152
VpaK11BI GGWCC 1 cut(s) 719
VspI ATTAAT 1 cut(s) 534
XapI RAATTY 1 cut(s) 638
XceI RCATGY 1 cut(s) 455
ZrmI AGTACT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.