pycom06g15380

F-box protein SKIP16-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014736
Physical Location & Seq
Reverse (-)
19915642 .. 19916667
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 591 bp
ATGCATTCATTAAGCCTCAAAGTCTGCAGTGATTGCTGTCCGGGGACGACGAGAACGTGTTTTCCGGGTCGACGGAGCTTCAGCGTCGTGAATTTGGAGGCCCGGAACTTGAGAGTCGGTTCGGGGCGTGGGGATTTCAGGAAATCGGGTCGGGTCGTGGCGTGCGCCGCGGAGAGGAGCGAGGGGAATGGGTGGAGTGGAACTCCGAGTTCGAGTCCGAGTTCCGGTGCGGGCCAGAAACCGAGTTCGTTTTTGTCTCGGACTCAGAGCTGGGCTTTGTTGAAGCAGCAAATGGAGGTCGCGGCGAAATCCGAGGATTATGAAGAGGCGGCGAGGATAAGGGACTCGTTGAAGTTGCTGGAAGAAGAAGAGCCGGTTTTGTGGCTCCGAAGAAGGATTAAGGAGGCAATTGCTGAAGAGAGATTTGAGGAGGCCGCGAAATATCGAGATGAGCTAAAGGAAATTGCCCCTTACTCTTTCTTGAAATGTTCAAGTGATGCAACAACTTTGGGTATTCGTGTTCAAGTTAGGAGCGTGTACATAGAGGGTAGAAGTCAGCCTTCGAAGGGGCAACACTTCTTTGCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF525 UVR

Protein Analysis

197

Amino Acids

21.86

Weight (kDa)

9.08

Isoelectric Point (pI)

66.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UVR PF02151 94 - 122 6.7e-07 UvrB/uvrC motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 70
AccII CGCG 3 cut(s) 170, 302, 437
AciI CCGC 6 cut(s) 168, 170, 230, 302, 329, 435
AcsI RAATTY 1 cut(s) 91
AcuI CTGAAG 2 cut(s) 64, 435
AfaI GTAC 1 cut(s) 539
AfiI CCNNNNNNNGG 3 cut(s) 174, 224, 566
AflIII ACRYGT 1 cut(s) 56
AgsI TTSAA 5 cut(s) 283, 352, 484, 492, 524
AloI GAACNNNNNNTCC 2 cut(s) 46, 78
AluBI AGCT 3 cut(s) 78, 270, 454
AluI AGCT 3 cut(s) 78, 270, 454
Alw26I GTCTC 1 cut(s) 261
AoxI GGCC 3 cut(s) 99, 232, 432
ApeKI GCWGC 1 cut(s) 286
ApoI RAATTY 1 cut(s) 91
AspLEI GCGC 1 cut(s) 167
AspS9I GGNCC 2 cut(s) 100, 232
AsuC2I CCSGG 3 cut(s) 42, 66, 103
AsuII TTCGAA 1 cut(s) 563
BbvI GCAGC 1 cut(s) 298
BcnI CCSGG 3 cut(s) 42, 66, 103
BcoDI GTCTC 1 cut(s) 261
BfmI CTRYAG 1 cut(s) 25
BisI GCNGC 5 cut(s) 168, 287, 303, 330, 435
BlsI GCNGC 5 cut(s) 169, 288, 304, 331, 436
Bme1390I CCNGG 3 cut(s) 42, 66, 103
BmgT120I GGNCC 2 cut(s) 100, 232
BmiI GGNNCC 1 cut(s) 386
BmrFI CCNGG 3 cut(s) 42, 66, 103
BmsI GCATC 1 cut(s) 487
BplI GAGNNNNNCTC 2 cut(s) 187, 219
Bpu14I TTCGAA 1 cut(s) 563
BpuEI CTTGAG 1 cut(s) 130
BpuMI CCSGG 3 cut(s) 42, 66, 103
BsaJI CCNNGG 3 cut(s) 41, 168, 312
BsaWI WCCGGW 1 cut(s) 224
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 224, 566
Bse118I RCCGGY 1 cut(s) 373
BseDI CCNNGG 3 cut(s) 41, 168, 312
BseLI CCNNNNNNNGG 3 cut(s) 174, 224, 566
BseMII CTCAG 1 cut(s) 278
BseRI GAGGAG 2 cut(s) 190, 443
BseXI GCAGC 1 cut(s) 298
BseYI CCCAGC 1 cut(s) 270
Bsh1236I CGCG 3 cut(s) 170, 302, 437
BshFI GGCC 3 cut(s) 101, 234, 434
BsiSI CCGG 5 cut(s) 41, 65, 103, 225, 374
BslFI GGGAC 2 cut(s) 58, 356
BslI CCNNNNNNNGG 3 cut(s) 174, 224, 566
BsmAI GTCTC 1 cut(s) 261
BsmFI GGGAC 2 cut(s) 58, 356
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 3 cut(s) 101, 234, 434
Bsp119I TTCGAA 1 cut(s) 563
Bsp1407I TGTACA 1 cut(s) 537
BspACI CCGC 6 cut(s) 168, 170, 230, 302, 329, 435
BspANI GGCC 3 cut(s) 101, 234, 434
BspCNI CTCAG 1 cut(s) 277
BspFNI CGCG 3 cut(s) 170, 302, 437
BspLI GGNNCC 1 cut(s) 386
BspMAI CTGCAG 1 cut(s) 29
BspQI GCTCTTC 1 cut(s) 363
BspT104I TTCGAA 1 cut(s) 563
BsrFI RCCGGY 1 cut(s) 373
BsrGI TGTACA 1 cut(s) 537
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 3 cut(s) 41, 168, 312
Bst6I CTCTTC 3 cut(s) 318, 363, 411
BstAPI GCANNNNNTGC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 537
BstBI TTCGAA 1 cut(s) 563
BstC8I GCNNGC 2 cut(s) 163, 232
BstDEI CTNAG 1 cut(s) 264
BstDSI CCRYGG 1 cut(s) 168
BstFNI CGCG 3 cut(s) 170, 302, 437
BstHHI GCGC 1 cut(s) 167
BstMAI GTCTC 1 cut(s) 261
BstMWI GCNNNNNNNGC 2 cut(s) 33, 167
BstSCI CCNGG 3 cut(s) 40, 64, 101
BstSFI CTRYAG 1 cut(s) 25
BstUI CGCG 3 cut(s) 170, 302, 437
BstV1I GCAGC 1 cut(s) 298
BsuRI GGCC 3 cut(s) 101, 234, 434
BtgI CCRYGG 1 cut(s) 168
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 1 cut(s) 34
Cac8I GCNNGC 2 cut(s) 163, 232
CfoI GCGC 1 cut(s) 167
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 2 cut(s) 100, 232
Cfr42I CCGCGG 1 cut(s) 171
CseI GACGC 1 cut(s) 73
Csp6I GTAC 1 cut(s) 538
CviQI GTAC 1 cut(s) 538
DdeI CTNAG 1 cut(s) 264
Eam1104I CTCTTC 3 cut(s) 318, 363, 411
EarI CTCTTC 3 cut(s) 318, 363, 411
Eco57I CTGAAG 2 cut(s) 64, 435
EcoT22I ATGCAT 1 cut(s) 6
FaiI YATR 3 cut(s) 321, 542, 586
FalI AAGNNNNNCTT 2 cut(s) 544, 576
FaqI GGGAC 2 cut(s) 58, 356
FauI CCCGC 1 cut(s) 223
FblI GTMKAC 1 cut(s) 70
Fnu4HI GCNGC 5 cut(s) 168, 287, 303, 330, 435
Fsp4HI GCNGC 5 cut(s) 168, 287, 303, 330, 435
GlaI GCGC 1 cut(s) 166
GluI GCNGC 5 cut(s) 168, 287, 303, 330, 435
GsaI CCCAGC 1 cut(s) 274
HaeIII GGCC 3 cut(s) 101, 234, 434
HapII CCGG 5 cut(s) 41, 65, 103, 225, 374
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 167
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 1 cut(s) 71
HindII GTYRAC 1 cut(s) 71
HinfI GANTC 4 cut(s) 114, 214, 262, 344
HpaII CCGG 5 cut(s) 41, 65, 103, 225, 374
Hpy166II GTNNAC 2 cut(s) 71, 538
Hpy188I TCNGA 6 cut(s) 207, 219, 261, 267, 313, 389
Hpy188III TCNNGA 4 cut(s) 88, 139, 446, 481
Hpy8I GTNNAC 2 cut(s) 71, 538
Hpy99I CGWCG 3 cut(s) 52, 75, 89
HpyAV CCTTC 3 cut(s) 387, 559, 570
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 4 cut(s) 4, 27, 500, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 167
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 1 cut(s) 56
HspAI GCGC 1 cut(s) 165
KspI CCGCGG 1 cut(s) 171
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 4 cut(s) 75, 177, 390, 531
LpnPI CCDG 9 cut(s) 54, 78, 116, 124, 238, 248, 256, 344, 387
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 1 cut(s) 487
MaeII ACGT 1 cut(s) 56
MboII GAAGA 6 cut(s) 335, 374, 377, 380, 402, 428
MfeI CAATTG 1 cut(s) 408
MluCI AATT 3 cut(s) 91, 408, 462
MlyI GAGTC 4 cut(s) 123, 223, 256, 338
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 11, 399
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 5 cut(s) 41, 65, 103, 225, 374
MspR9I CCNGG 3 cut(s) 42, 66, 103
MunI CAATTG 1 cut(s) 408
Mva1269I GAATGC 1 cut(s) 4
MvnI CGCG 3 cut(s) 170, 302, 437
MwoI GCNNNNNNNGC 2 cut(s) 33, 167
NciI CCSGG 3 cut(s) 42, 66, 103
NlaIV GGNNCC 1 cut(s) 386
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 563
PciSI GCTCTTC 1 cut(s) 363
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PctI GAATGC 1 cut(s) 4
PkrI GCNGC 5 cut(s) 169, 288, 304, 331, 436
PleI GAGTC 4 cut(s) 122, 222, 256, 338
PpsI GAGTC 4 cut(s) 122, 222, 256, 338
PspFI CCCAGC 1 cut(s) 270
PspN4I GGNNCC 1 cut(s) 386
PspPI GGNCC 2 cut(s) 100, 232
PstI CTGCAG 1 cut(s) 29
RsaI GTAC 1 cut(s) 539
RsaNI GTAC 1 cut(s) 538
SacII CCGCGG 1 cut(s) 171
SalI GTCGAC 1 cut(s) 69
SapI GCTCTTC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 11, 399
SatI GCNGC 5 cut(s) 168, 287, 303, 330, 435
Sau96I GGNCC 2 cut(s) 100, 232
SchI GAGTC 4 cut(s) 123, 223, 256, 338
ScrFI CCNGG 3 cut(s) 42, 66, 103
SetI ASST 5 cut(s) 59, 80, 272, 300, 456
SfaNI GCATC 1 cut(s) 487
SfcI CTRYAG 1 cut(s) 25
Sfr303I CCGCGG 1 cut(s) 171
SfuI TTCGAA 1 cut(s) 563
SgrBI CCGCGG 1 cut(s) 171
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
Sse9I AATT 3 cut(s) 91, 408, 462
SsiI CCGC 6 cut(s) 168, 170, 230, 302, 329, 435
StyD4I CCNGG 3 cut(s) 40, 64, 101
TaiI ACGT 1 cut(s) 59
TaqI TCGA 4 cut(s) 70, 212, 445, 563
TasI AATT 3 cut(s) 91, 408, 462
TatI WGTACW 1 cut(s) 537
TauI GCSGC 4 cut(s) 170, 305, 332, 437
Tru1I TTAA 2 cut(s) 11, 399
Tru9I TTAA 2 cut(s) 11, 399
TscAI CASTG 1 cut(s) 34
TseI GCWGC 1 cut(s) 286
TspDTI ATGAA 1 cut(s) 336
TspGWI ACGGA 1 cut(s) 88
TspRI CASTG 1 cut(s) 34
XapI RAATTY 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 70
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.