pycom06g18180

COBRA-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0018533
Physical Location & Seq
Forward (+)
22017194 .. 22019281
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 519 bp
ATGGAGCCTCAGTGCTTCTCAGCCGTTGGATCCATGGCAAAGCTGACCAGCTTCACCATTCTGCTCGTCTTCTTGCTTTCCTGCTTCACTTTTACTTCTACAGAAGCCTATGATGCTCTTGATCCAAATGGGAATATTACAATTAAATGGGATGTCATCAGTTGGACTCCGGATGGTTATCTTGCTGTAGTTACAATGTTTAACTTCCAGCAGTATCGGCATATACAAGCGCCAGGTTGGACATTGGGATGGACATGGGCAAAAAAGGAAGTAATCTGGAGCATGGTGGGAGCACAAGCAACAGAGCAAGGGGATTGCTCGAAATACAAAGGGAACGTCCCACATTGCTGTAAGAAGGATCGATCAACAAACCACTGTTGTGGATTTGTTGCCAGGAACCCCCTACAATCAGCAGATTGCAAACTGCTGCAAGGGCGGAGTCATGCAATCATGGATCCAGGACCCAGACAATGCCATAAGTTCGTTTCAGGTCAGTGTGGGTGCTGCTGGAACAACTAA

Protein Analysis

173

Amino Acids

19.23

Weight (kDa)

8.26

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COBRA PF04833 60 - 120 1.4e-25 COBRA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018533)

Species Orthologous Gene IDs
pyrus_communis pycom06g18180
rosa_chinensis RchiOBHm_Chr2g0130691
rosa_multiflora Rmu_co8433061.1_g000001 Rmu_sc0002279.1_g000054 Rmu_sc0041315.1_g000001
rosa_roxburghii Rroxscaffold_2G00113710
rosa_samantha Rh2AG344100 Rh2CG331300 Rh2DG370600
rosa_wichuraiana Rw2G027550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 169
AciI CCGC 1 cut(s) 436
AclWI GGATC 6 cut(s) 24, 37, 116, 366, 449, 462
AjnI CCWGG 3 cut(s) 232, 392, 457
AleI CACNNNNGTG 1 cut(s) 378
AluBI AGCT 2 cut(s) 43, 51
AluI AGCT 2 cut(s) 43, 51
Alw21I GWGCWC 1 cut(s) 295
AlwI GGATC 6 cut(s) 24, 37, 116, 366, 449, 462
Aor13HI TCCGGA 1 cut(s) 169
ApeKI GCWGC 2 cut(s) 427, 504
ArsI GACNNNNNNTTYG 2 cut(s) 321, 353
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 1 cut(s) 461
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 1 cut(s) 461
BamHI GGATCC 2 cut(s) 29, 454
BbsI GAAGAC 1 cut(s) 61
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 2 cut(s) 414, 491
BccI CCATC 2 cut(s) 167, 243
BceAI ACGGC 1 cut(s) 8
BciT130I CCWGG 3 cut(s) 234, 394, 459
BfmI CTRYAG 2 cut(s) 99, 186
BfoI RGCGCY 1 cut(s) 233
BisI GCNGC 2 cut(s) 428, 505
BlsI GCNGC 2 cut(s) 429, 506
Bme1390I CCNGG 3 cut(s) 234, 394, 459
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 1 cut(s) 461
BmiI GGNNCC 5 cut(s) 6, 31, 398, 456, 463
BmrFI CCNGG 3 cut(s) 234, 394, 459
BmsI GCATC 1 cut(s) 103
BpiI GAAGAC 1 cut(s) 61
BpmI CTGGAG 1 cut(s) 298
Bsa29I ATCGAT 1 cut(s) 361
BsaBI GATNNNNATC 1 cut(s) 177
BsaJI CCNNGG 1 cut(s) 33
BsaWI WCCGGW 1 cut(s) 169
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse3DI GCAATG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 177
BseAI TCCGGA 1 cut(s) 169
BseBI CCWGG 3 cut(s) 234, 394, 459
BseCI ATCGAT 1 cut(s) 361
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 3 cut(s) 157, 178, 254
BseJI GATNNNNATC 1 cut(s) 177
BseMI GCAATG 1 cut(s) 343
BseMII CTCAG 2 cut(s) 23, 33
BseXI GCAGC 2 cut(s) 414, 491
BshVI ATCGAT 1 cut(s) 361
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 170
BslFI GGGAC 1 cut(s) 323
BsmFI GGGAC 1 cut(s) 323
Bsp1286I GDGCHC 1 cut(s) 295
Bsp13I TCCGGA 1 cut(s) 169
Bsp143I GATC 5 cut(s) 29, 121, 358, 362, 454
Bsp19I CCATGG 1 cut(s) 33
BspACI CCGC 1 cut(s) 436
BspCNI CTCAG 2 cut(s) 22, 32
BspDI ATCGAT 1 cut(s) 361
BspEI TCCGGA 1 cut(s) 169
BspLI GGNNCC 5 cut(s) 6, 31, 398, 456, 463
BspPI GGATC 6 cut(s) 24, 37, 116, 366, 449, 462
BsrDI GCAATG 1 cut(s) 343
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 5 cut(s) 29, 121, 358, 362, 454
BssT1I CCWWGG 1 cut(s) 33
Bst2UI CCWGG 3 cut(s) 234, 394, 459
Bst4CI ACNGT 1 cut(s) 377
BstDEI CTNAG 2 cut(s) 9, 19
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 3 cut(s) 157, 178, 254
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 5 cut(s) 32, 124, 361, 365, 457
BstMBI GATC 5 cut(s) 29, 121, 358, 362, 454
BstMWI GCNNNNNNNGC 3 cut(s) 113, 217, 433
BstNI CCWGG 3 cut(s) 234, 394, 459
BstSCI CCNGG 3 cut(s) 232, 392, 457
BstSFI CTRYAG 2 cut(s) 99, 186
BstV1I GCAGC 2 cut(s) 414, 491
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 2 cut(s) 29, 454
BstXI CCANNNNNNTGG 1 cut(s) 380
BstYI RGATCY 2 cut(s) 29, 454
Bsu15I ATCGAT 1 cut(s) 361
BsuTUI ATCGAT 1 cut(s) 361
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 3 cut(s) 157, 178, 254
BtsIMutI CAGTG 3 cut(s) 17, 373, 500
CfoI GCGC 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 461
ClaI ATCGAT 1 cut(s) 361
CviAII CATG 5 cut(s) 34, 255, 283, 443, 451
CviJI RGCY 5 cut(s) 7, 23, 43, 51, 107
CviKI_1 RGCY 5 cut(s) 7, 23, 43, 51, 107
DdeI CTNAG 2 cut(s) 9, 19
DpnI GATC 5 cut(s) 31, 123, 360, 364, 456
DpnII GATC 5 cut(s) 29, 121, 358, 362, 454
EciI GGCGGA 1 cut(s) 451
Eco130I CCWWGG 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 461
EcoO109I RGGNCCY 1 cut(s) 461
EcoRII CCWGG 3 cut(s) 232, 392, 457
EcoT14I CCWWGG 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 33
FaeI CATG 5 cut(s) 37, 258, 286, 446, 454
FaiI YATR 9 cut(s) 35, 111, 222, 224, 256, 284, 444, 452, 477
FaqI GGGAC 1 cut(s) 323
FatI CATG 5 cut(s) 33, 254, 282, 442, 450
Fnu4HI GCNGC 2 cut(s) 428, 505
FokI GGATG 3 cut(s) 164, 185, 261
Fsp4HI GCNGC 2 cut(s) 428, 505
GlaI GCGC 1 cut(s) 231
GluI GCNGC 2 cut(s) 428, 505
GsuI CTGGAG 1 cut(s) 298
HaeII RGCGCY 1 cut(s) 233
HapII CCGG 1 cut(s) 170
HhaI GCGC 1 cut(s) 232
Hin1II CATG 5 cut(s) 37, 258, 286, 446, 454
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 2 cut(s) 166, 439
HpaII CCGG 1 cut(s) 170
HphI GGTGA 1 cut(s) 46
Hpy188III TCNNGA 3 cut(s) 119, 170, 277
HpyAV CCTTC 1 cut(s) 349
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 3 cut(s) 420, 430, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 217, 433
HpyF3I CTNAG 2 cut(s) 9, 19
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 5 cut(s) 37, 258, 286, 446, 454
HspAI GCGC 1 cut(s) 230
Kpn2I TCCGGA 1 cut(s) 169
Kzo9I GATC 5 cut(s) 29, 121, 358, 362, 454
LmnI GCTCC 3 cut(s) 4, 279, 290
Lsp1109I GCAGC 2 cut(s) 414, 491
LweI GCATC 1 cut(s) 103
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 1 cut(s) 190
MalI GATC 5 cut(s) 31, 123, 360, 364, 456
MboI GATC 5 cut(s) 29, 121, 358, 362, 454
MboII GAAGA 1 cut(s) 61
MflI RGATCY 2 cut(s) 29, 454
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 1 cut(s) 141
MlyI GAGTC 2 cut(s) 160, 448
MmeI TCCRAC 3 cut(s) 7, 143, 218
MnlI CCTC 1 cut(s) 18
MroI TCCGGA 1 cut(s) 169
MseI TTAA 2 cut(s) 144, 201
MslI CAYNNNNRTG 2 cut(s) 247, 378
MspI CCGG 1 cut(s) 170
MspR9I CCNGG 3 cut(s) 234, 394, 459
MvaI CCWGG 3 cut(s) 234, 394, 459
MwoI GCNNNNNNNGC 3 cut(s) 113, 217, 433
NcoI CCATGG 1 cut(s) 33
NdeII GATC 5 cut(s) 29, 121, 358, 362, 454
NlaIII CATG 5 cut(s) 37, 258, 286, 446, 454
NlaIV GGNNCC 5 cut(s) 6, 31, 398, 456, 463
OliI CACNNNNGTG 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 457
PkrI GCNGC 2 cut(s) 429, 506
PleI GAGTC 2 cut(s) 160, 447
PpsI GAGTC 2 cut(s) 160, 447
PpuMI RGGWCCY 1 cut(s) 461
Psp5II RGGWCCY 1 cut(s) 461
Psp6I CCWGG 3 cut(s) 232, 392, 457
PspGI CCWGG 3 cut(s) 232, 392, 457
PspN4I GGNNCC 5 cut(s) 6, 31, 398, 456, 463
PspPI GGNCC 1 cut(s) 461
PspPPI RGGWCCY 1 cut(s) 461
PsuI RGATCY 2 cut(s) 29, 454
RseI CAYNNNNRTG 2 cut(s) 247, 378
SaqAI TTAA 2 cut(s) 144, 201
SatI GCNGC 2 cut(s) 428, 505
Sau3AI GATC 5 cut(s) 29, 121, 358, 362, 454
Sau96I GGNCC 1 cut(s) 461
SchI GAGTC 2 cut(s) 160, 448
ScrFI CCNGG 3 cut(s) 234, 394, 459
SduI GDGCHC 1 cut(s) 295
SetI ASST 5 cut(s) 45, 53, 238, 339, 493
SfaNI GCATC 1 cut(s) 103
SfcI CTRYAG 2 cut(s) 99, 186
SinI GGWCC 1 cut(s) 461
SmiMI CAYNNNNRTG 2 cut(s) 247, 378
Sse9I AATT 1 cut(s) 141
SsiI CCGC 1 cut(s) 436
SspI AATATT 1 cut(s) 136
StyD4I CCNGG 3 cut(s) 232, 392, 457
StyI CCWWGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 1 cut(s) 339
TaqI TCGA 2 cut(s) 320, 361
TasI AATT 1 cut(s) 141
Tru1I TTAA 2 cut(s) 144, 201
Tru9I TTAA 2 cut(s) 144, 201
TscAI CASTG 3 cut(s) 17, 380, 500
TseI GCWGC 2 cut(s) 427, 504
TspRI CASTG 3 cut(s) 17, 380, 500
VpaK11BI GGWCC 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.