Rmu_sc0002279.1_g000054

COBRA-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002279.1
OG0018533
Physical Location & Seq
Reverse (-)
228692 .. 232124
3433 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002279.1_g000054.1.cds

Sequence Viewer

Length: 1404 bp
atgggttgccttggaaaatcgattgggggagtttctggggttttcgaggtcggttgggttcaactggggttactgggatttgaagtttctgagcaagaaggagccggcagacaggaagaccggtactgtgtagaagaacaggcctatgatccattggatcccaatggaaacattactattcgttgggatataatgaattgggcgccagatggatacgaggcaaccatcacaatgtttaacttccagaaatatcggcatatcgaacagccaggttggacattaggatggacctgggcaaaaaaagaattcatatggtctatgctaggagggaaaacaatagaccgaggggactgctcaagtttcacaggacccactccacactgttgcaagaagaatccacaagttgtggacttacttcctgatgtgcctattgatgagcaggtcggaagttgttgtaggggaggagtcatgagctcgttgattcaggacacgagcaaagctgtcagcagattccagattactgtgggtggtgccggaagtagcaagagcactatcaaagtccccaagaaatttacactgagagcaccggggccaggatacacgtgtggggcagcagagattgtgaaaccaaccattttttactcatctgatggaaggagagcgctgccagccttcaaaacttggaatgtaacatgcatgtactctccgttcctagctacagagttccccacctgttgtgtgtcattaagtgcattttacgatgacaaaatagtagactgcccaaagtgtggttgtggttgtcagagagcaaaagttggtagttgtgtagagtcaagctctccttactcaggatcagttcctgaagaatctagtatctctcccctcatccagtgttcgaatcatatgtgtccggtgcgtgttcactggcacattaaacagaattacaggggctattggagggtacaaattacaataaccaacttcaacctcaacaagaactactctgaatggaatgctgtcgtccaacatccaaattttaataatttgacacgcattttctctttcaattactatccactagccccttatgatggattcaatgacactgccatgctgtggggacttccttacttcaatgatattctcctacaagctggccctcaaggatacgtgcagtcggaaatcctactaggcagggataggtctgcttttacttgggaaaatggttgggctttccctcggaggatctatttcaatggtgatatgtgtgtaatgccagacgcatatcctcaattaccggctccaactcctagaccaaatcttccagtacttgatccagcactagtcattatggtggctgtaatttgttatatattggtaggggccttttgtaagattgtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

467

Amino Acids

52.03

Weight (kDa)

6.59

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018533)

Species Orthologous Gene IDs
pyrus_communis pycom06g18180
rosa_chinensis RchiOBHm_Chr2g0130691
rosa_multiflora Rmu_co8433061.1_g000001 Rmu_sc0002279.1_g000054 Rmu_sc0041315.1_g000001
rosa_roxburghii Rroxscaffold_2G00113710
rosa_samantha Rh2AG344100 Rh2CG331300 Rh2DG370600
rosa_wichuraiana Rw2G027550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 430
AccB1I GGYRCC 2 cut(s) 202, 530
AccB7I CCANNNNNTGG 2 cut(s) 788, 1116
AccI GTMKAC 1 cut(s) 774
AclWI GGATC 6 cut(s) 143, 152, 165, 859, 1253, 1328
AcsI RAATTY 3 cut(s) 305, 569, 1033
AcuI CTGAAG 1 cut(s) 882
AcvI CACGTG 1 cut(s) 603
AcyI GRCGYC 1 cut(s) 203
AfaI GTAC 4 cut(s) 125, 701, 963, 1329
AfeI AGCGCT 1 cut(s) 663
AfiI CCNNNNNNNGG 5 cut(s) 593, 788, 848, 1091, 1116
AflIII ACRYGT 2 cut(s) 600, 602
AgeI ACCGGT 1 cut(s) 120
AgsI TTSAA 8 cut(s) 62, 83, 676, 985, 1066, 1099, 1135, 1255
AhlI ACTAGT 1 cut(s) 1342
AjnI CCWGG 3 cut(s) 268, 290, 592
AluBI AGCT 5 cut(s) 474, 500, 716, 837, 1154
AluI AGCT 5 cut(s) 474, 500, 716, 837, 1154
Alw21I GWGCWC 3 cut(s) 476, 551, 586
AlwI GGATC 6 cut(s) 143, 152, 165, 859, 1253, 1328
AlwNI CAGNNNCTG 1 cut(s) 860
Aor51HI AGCGCT 1 cut(s) 663
AoxI GGCC 4 cut(s) 141, 590, 1156, 1383
ApeKI GCWGC 2 cut(s) 611, 664
ApoI RAATTY 3 cut(s) 305, 569, 1033
AsiGI ACCGGT 1 cut(s) 120
AspLEI GCGC 2 cut(s) 205, 664
AspS9I GGNCC 5 cut(s) 288, 368, 590, 1157, 1383
AsuC2I CCSGG 1 cut(s) 588
AsuHPI GGTGA 1 cut(s) 1271
AsuII TTCGAA 1 cut(s) 896
AvaII GGWCC 2 cut(s) 288, 368
BaeI ACNNNNGTAYC 2 cut(s) 589, 622
BamHI GGATCC 1 cut(s) 157
BanI GGYRCC 2 cut(s) 202, 530
BanII GRGCYC 1 cut(s) 476
BauI CACGAG 1 cut(s) 490
BbrPI CACGTG 1 cut(s) 603
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 3 cut(s) 476, 551, 586
BbvI GCAGC 2 cut(s) 623, 651
BccI CCATC 5 cut(s) 203, 233, 279, 644, 1085
BcgI CGANNNNNNTGC 2 cut(s) 333, 367
BciT130I CCWGG 3 cut(s) 270, 292, 594
BciVI GTATCC 3 cut(s) 206, 590, 1160
BcnI CCSGG 1 cut(s) 588
BcuI ACTAGT 1 cut(s) 1342
BfaI CTAG 7 cut(s) 323, 713, 870, 1079, 1190, 1311, 1343
BfmI CTRYAG 1 cut(s) 717
BfoI RGCGCY 2 cut(s) 206, 665
BfuAI ACCTGC 1 cut(s) 430
BfuI GTATCC 3 cut(s) 206, 590, 1160
BisI GCNGC 2 cut(s) 612, 665
BlsI GCNGC 2 cut(s) 613, 666
BmcAI AGTACT 1 cut(s) 1329
Bme1390I CCNGG 4 cut(s) 270, 292, 588, 594
Bme18I GGWCC 2 cut(s) 288, 368
BmgT120I GGNCC 5 cut(s) 288, 368, 590, 1157, 1383
BmiI GGNNCC 8 cut(s) 103, 159, 204, 370, 532, 591, 1302, 1384
BmrFI CCNGG 4 cut(s) 270, 292, 588, 594
BmrI ACTGGG 2 cut(s) 74, 83
BmuI ACTGGG 2 cut(s) 74, 83
BpiI GAAGAC 1 cut(s) 123
BplI GAGNNNNNCTC 2 cut(s) 821, 853
Bpu14I TTCGAA 1 cut(s) 896
BpuEI CTTGAG 2 cut(s) 340, 1146
BpuMI CCSGG 1 cut(s) 588
Bsa29I ATCGAT 1 cut(s) 20
BsaAI YACGTR 2 cut(s) 603, 1171
BsaHI GRCGYC 1 cut(s) 203
BsaJI CCNNGG 5 cut(s) 10, 291, 343, 587, 1238
BsaWI WCCGGW 2 cut(s) 120, 910
Bsc4I CCNNNNNNNGG 5 cut(s) 593, 788, 848, 1091, 1116
Bse118I RCCGGY 3 cut(s) 104, 120, 1297
Bse1I ACTGG 5 cut(s) 69, 78, 889, 929, 1325
BseBI CCWGG 3 cut(s) 270, 292, 594
BseCI ATCGAT 1 cut(s) 20
BseDI CCNNGG 5 cut(s) 10, 291, 343, 587, 1238
BseGI GGATG 3 cut(s) 290, 885, 1027
BseLI CCNNNNNNNGG 5 cut(s) 593, 788, 848, 1091, 1116
BseMII CTCAG 3 cut(s) 81, 569, 861
BseNI ACTGG 5 cut(s) 69, 78, 889, 929, 1325
BseRI GAGGAG 1 cut(s) 477
BseXI GCAGC 2 cut(s) 623, 651
BsgI GTGCAG 1 cut(s) 1193
BshFI GGCC 4 cut(s) 143, 592, 1158, 1385
BshNI GGYRCC 2 cut(s) 202, 530
BshTI ACCGGT 1 cut(s) 120
BshVI ATCGAT 1 cut(s) 20
BsiHKAI GWGCWC 3 cut(s) 476, 551, 586
BsiSI CCGG 6 cut(s) 105, 121, 534, 587, 911, 1298
BslFI GGGAC 3 cut(s) 362, 545, 1134
BslI CCNNNNNNNGG 5 cut(s) 593, 788, 848, 1091, 1116
BsmFI GGGAC 3 cut(s) 362, 545, 1134
BsmI GAATGC 1 cut(s) 1018
BsnI GGCC 4 cut(s) 143, 592, 1158, 1385
Bsp119I TTCGAA 1 cut(s) 896
Bsp1286I GDGCHC 3 cut(s) 476, 551, 586
Bsp143I GATC 5 cut(s) 148, 157, 851, 1245, 1333
BspANI GGCC 4 cut(s) 143, 592, 1158, 1385
BspCNI CTCAG 3 cut(s) 82, 570, 860
BspDI ATCGAT 1 cut(s) 20
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 8 cut(s) 103, 159, 204, 370, 532, 591, 1302, 1384
BspMI ACCTGC 1 cut(s) 430
BspPI GGATC 6 cut(s) 143, 152, 165, 859, 1253, 1328
BspT104I TTCGAA 1 cut(s) 896
BspT107I GGYRCC 2 cut(s) 202, 530
BsrFI RCCGGY 3 cut(s) 104, 120, 1297
BsrI ACTGG 5 cut(s) 69, 78, 889, 929, 1325
BssAI RCCGGY 3 cut(s) 104, 120, 1297
BssECI CCNNGG 5 cut(s) 10, 291, 343, 587, 1238
BssMI GATC 5 cut(s) 148, 157, 851, 1245, 1333
BssNI GRCGYC 1 cut(s) 203
BssSI CACGAG 1 cut(s) 490
BssT1I CCWWGG 1 cut(s) 10
Bst2BI CACGAG 1 cut(s) 490
Bst2UI CCWGG 3 cut(s) 270, 292, 594
Bst4CI ACNGT 3 cut(s) 128, 383, 523
BstACI GRCGYC 1 cut(s) 203
BstBAI YACGTR 2 cut(s) 603, 1171
BstBI TTCGAA 1 cut(s) 896
BstC8I GCNNGC 3 cut(s) 106, 669, 1156
BstDEI CTNAG 3 cut(s) 90, 578, 847
BstENI CCTNNNNNAGG 1 cut(s) 846
BstF5I GGATG 3 cut(s) 290, 885, 1027
BstH2I RGCGCY 2 cut(s) 206, 665
BstHHI GCGC 2 cut(s) 205, 664
BstKTI GATC 5 cut(s) 151, 160, 854, 1248, 1336
BstMBI GATC 5 cut(s) 148, 157, 851, 1245, 1333
BstMWI GCNNNNNNNGC 1 cut(s) 668
BstNI CCWGG 3 cut(s) 270, 292, 594
BstNSI RCATGY 2 cut(s) 696, 700
BstSCI CCNGG 4 cut(s) 268, 290, 586, 592
BstSFI CTRYAG 1 cut(s) 717
BstV1I GCAGC 2 cut(s) 623, 651
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 2 cut(s) 157, 1245
BstYI RGATCY 2 cut(s) 157, 1245
Bsu15I ATCGAT 1 cut(s) 20
BsuI GTATCC 3 cut(s) 206, 590, 1160
BsuRI GGCC 4 cut(s) 143, 592, 1158, 1385
BsuTUI ATCGAT 1 cut(s) 20
BtsCI GGATG 3 cut(s) 290, 885, 1027
BtsI GCAGTG 1 cut(s) 1104
BtsIMutI CAGTG 5 cut(s) 379, 575, 896, 922, 1104
BveI ACCTGC 1 cut(s) 430
Cac8I GCNNGC 3 cut(s) 106, 669, 1156
CaiI CAGNNNCTG 1 cut(s) 860
CciI TCATGA 1 cut(s) 468
CfoI GCGC 2 cut(s) 205, 664
Cfr10I RCCGGY 3 cut(s) 104, 120, 1297
Cfr13I GGNCC 5 cut(s) 288, 368, 590, 1157, 1383
ClaI ATCGAT 1 cut(s) 20
CseI GACGC 1 cut(s) 1289
Csp6I GTAC 4 cut(s) 124, 700, 962, 1328
CspAI ACCGGT 1 cut(s) 120
CviAII CATG 4 cut(s) 469, 693, 697, 1111
CviQI GTAC 4 cut(s) 124, 700, 962, 1328
DdeI CTNAG 3 cut(s) 90, 578, 847
DinI GGCGCC 1 cut(s) 204
DpnI GATC 5 cut(s) 150, 159, 853, 1247, 1335
DpnII GATC 5 cut(s) 148, 157, 851, 1245, 1333
Ecl136II GAGCTC 1 cut(s) 474
Eco130I CCWWGG 1 cut(s) 10
Eco147I AGGCCT 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 288, 368
Eco47III AGCGCT 1 cut(s) 663
Eco53kI GAGCTC 1 cut(s) 474
Eco57I CTGAAG 1 cut(s) 882
Eco72I CACGTG 1 cut(s) 603
EcoICRI GAGCTC 1 cut(s) 474
EcoNI CCTNNNNNAGG 1 cut(s) 846
EcoO109I RGGNCCY 2 cut(s) 368, 1383
EcoRI GAATTC 1 cut(s) 305
EcoRII CCWGG 3 cut(s) 268, 290, 592
EcoT14I CCWWGG 1 cut(s) 10
EcoT22I ATGCAT 1 cut(s) 698
EcoT38I GRGCYC 1 cut(s) 476
EgeI GGCGCC 1 cut(s) 204
EheI GGCGCC 1 cut(s) 204
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 4 cut(s) 472, 696, 700, 1114
FalI AAGNNNNNCTT 2 cut(s) 826, 858
FaqI GGGAC 3 cut(s) 362, 545, 1134
FatI CATG 4 cut(s) 468, 692, 696, 1110
FauNDI CATATG 2 cut(s) 311, 903
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 2 cut(s) 612, 665
FokI GGATG 3 cut(s) 297, 872, 1014
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 2 cut(s) 612, 665
FspBI CTAG 7 cut(s) 323, 713, 870, 1079, 1190, 1311, 1343
GlaI GCGC 2 cut(s) 204, 663
GluI GCNGC 2 cut(s) 612, 665
HaeII RGCGCY 2 cut(s) 206, 665
HaeIII GGCC 4 cut(s) 143, 592, 1158, 1385
HapII CCGG 6 cut(s) 105, 121, 534, 587, 911, 1298
HgaI GACGC 1 cut(s) 1289
HhaI GCGC 2 cut(s) 205, 664
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 4 cut(s) 472, 696, 700, 1114
Hin6I GCGC 2 cut(s) 203, 662
HinP1I GCGC 2 cut(s) 203, 662
HinfI GANTC 8 cut(s) 394, 465, 481, 510, 830, 866, 898, 1095
HpaII CCGG 6 cut(s) 105, 121, 534, 587, 911, 1298
HphI GGTGA 1 cut(s) 1271
Hpy166II GTNNAC 3 cut(s) 409, 775, 922
Hpy188I TCNGA 7 cut(s) 91, 446, 649, 804, 1006, 1180, 1242
Hpy188III TCNNGA 7 cut(s) 244, 419, 469, 485, 514, 849, 860
Hpy8I GTNNAC 3 cut(s) 409, 775, 922
HpyAV CCTTC 3 cut(s) 92, 648, 682
HpyCH4III ACNGT 3 cut(s) 128, 383, 523
HpyCH4IV ACGT 2 cut(s) 602, 1170
HpyCH4V TGCA 4 cut(s) 387, 696, 752, 1174
HpyF10VI GCNNNNNNNGC 1 cut(s) 668
HpyF3I CTNAG 3 cut(s) 90, 578, 847
HpySE526I ACGT 2 cut(s) 602, 1170
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 4 cut(s) 472, 696, 700, 1114
HspAI GCGC 2 cut(s) 203, 662
KasI GGCGCC 1 cut(s) 202
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Kzo9I GATC 5 cut(s) 148, 157, 851, 1245, 1333
LmnI GCTCC 2 cut(s) 101, 1306
Lsp1109I GCAGC 2 cut(s) 623, 651
MaeI CTAG 7 cut(s) 323, 713, 870, 1079, 1190, 1311, 1343
MaeII ACGT 2 cut(s) 602, 1170
MaeIII GTNAC 2 cut(s) 69, 688
MalI GATC 5 cut(s) 150, 159, 853, 1247, 1335
MboI GATC 5 cut(s) 148, 157, 851, 1245, 1333
MboII GAAGA 5 cut(s) 128, 146, 403, 875, 1313
MflI RGATCY 2 cut(s) 157, 1245
MhlI GDGCHC 3 cut(s) 476, 551, 586
Mly113I GGCGCC 1 cut(s) 203
MlyI GAGTC 2 cut(s) 474, 839
MmeI TCCRAC 5 cut(s) 254, 424, 1048, 1158, 1328
Mph1103I ATGCAT 1 cut(s) 698
MroNI GCCGGC 1 cut(s) 104
MseI TTAA 4 cut(s) 237, 746, 933, 1038
MslI CAYNNNNRTG 4 cut(s) 230, 283, 1109, 1352
MspI CCGG 6 cut(s) 105, 121, 534, 587, 911, 1298
MspR9I CCNGG 4 cut(s) 270, 292, 588, 594
Mva1269I GAATGC 1 cut(s) 1018
MvaI CCWGG 3 cut(s) 270, 292, 594
MwoI GCNNNNNNNGC 1 cut(s) 668
NaeI GCCGGC 1 cut(s) 106
NarI GGCGCC 1 cut(s) 203
NciI CCSGG 1 cut(s) 588
NdeI CATATG 2 cut(s) 311, 903
NdeII GATC 5 cut(s) 148, 157, 851, 1245, 1333
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 4 cut(s) 472, 696, 700, 1114
NlaIV GGNNCC 8 cut(s) 103, 159, 204, 370, 532, 591, 1302, 1384
NsiI ATGCAT 1 cut(s) 698
NspI RCATGY 2 cut(s) 696, 700
NspV TTCGAA 1 cut(s) 896
PagI TCATGA 1 cut(s) 468
PceI AGGCCT 1 cut(s) 143
PctI GAATGC 1 cut(s) 1018
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 6 cut(s) 394, 481, 510, 866, 898, 1095
PflMI CCANNNNNTGG 2 cut(s) 788, 1116
PinAI ACCGGT 1 cut(s) 120
PkrI GCNGC 2 cut(s) 613, 666
PleI GAGTC 2 cut(s) 473, 838
PluTI GGCGCC 1 cut(s) 206
PmaCI CACGTG 1 cut(s) 603
PmlI CACGTG 1 cut(s) 603
PpsI GAGTC 2 cut(s) 473, 838
Ppu21I YACGTR 2 cut(s) 603, 1171
PpuMI RGGWCCY 1 cut(s) 368
Psp124BI GAGCTC 1 cut(s) 476
Psp5II RGGWCCY 1 cut(s) 368
Psp6I CCWGG 3 cut(s) 268, 290, 592
PspCI CACGTG 1 cut(s) 603
PspGI CCWGG 3 cut(s) 268, 290, 592
PspN4I GGNNCC 8 cut(s) 103, 159, 204, 370, 532, 591, 1302, 1384
PspPI GGNCC 5 cut(s) 288, 368, 590, 1157, 1383
PspPPI RGGWCCY 1 cut(s) 368
PsrI GAACNNNNNNTAC 2 cut(s) 692, 724
PstNI CAGNNNCTG 1 cut(s) 860
PsuI RGATCY 2 cut(s) 157, 1245
RsaI GTAC 4 cut(s) 125, 701, 963, 1329
RsaNI GTAC 4 cut(s) 124, 700, 962, 1328
RseI CAYNNNNRTG 4 cut(s) 230, 283, 1109, 1352
SacI GAGCTC 1 cut(s) 476
SaqAI TTAA 4 cut(s) 237, 746, 933, 1038
SatI GCNGC 2 cut(s) 612, 665
Sau3AI GATC 5 cut(s) 148, 157, 851, 1245, 1333
Sau96I GGNCC 5 cut(s) 288, 368, 590, 1157, 1383
ScaI AGTACT 1 cut(s) 1329
SchI GAGTC 2 cut(s) 474, 839
ScrFI CCNGG 4 cut(s) 270, 292, 588, 594
SduI GDGCHC 3 cut(s) 476, 551, 586
SfcI CTRYAG 1 cut(s) 717
SfoI GGCGCC 1 cut(s) 204
SfuI TTCGAA 1 cut(s) 896
SinI GGWCC 2 cut(s) 288, 368
SmiMI CAYNNNNRTG 4 cut(s) 230, 283, 1109, 1352
SmlI CTYRAG 2 cut(s) 355, 1161
SmoI CTYRAG 2 cut(s) 355, 1161
SpeI ACTAGT 1 cut(s) 1342
SseBI AGGCCT 1 cut(s) 143
SspDI GGCGCC 1 cut(s) 202
SspMI CTAG 7 cut(s) 323, 713, 870, 1079, 1190, 1311, 1343
SstI GAGCTC 1 cut(s) 476
StuI AGGCCT 1 cut(s) 143
StyD4I CCNGG 4 cut(s) 268, 290, 586, 592
StyI CCWWGG 1 cut(s) 10
TaaI ACNGT 3 cut(s) 128, 383, 523
TaiI ACGT 2 cut(s) 605, 1173
TaqI TCGA 4 cut(s) 20, 45, 261, 896
TaqII GACCGA 1 cut(s) 357
TatI WGTACW 2 cut(s) 699, 1327
TfiI GAWTC 6 cut(s) 394, 481, 510, 866, 898, 1095
Tru1I TTAA 4 cut(s) 237, 746, 933, 1038
Tru9I TTAA 4 cut(s) 237, 746, 933, 1038
TscAI CASTG 5 cut(s) 386, 582, 896, 929, 1111
TseI GCWGC 2 cut(s) 611, 664
TspDTI ATGAA 2 cut(s) 209, 298
TspGWI ACGGA 1 cut(s) 696
TspRI CASTG 5 cut(s) 386, 582, 896, 929, 1111
Van91I CCANNNNNTGG 2 cut(s) 788, 1116
VpaK11BI GGWCC 2 cut(s) 288, 368
XagI CCTNNNNNAGG 1 cut(s) 846
XapI RAATTY 3 cut(s) 305, 569, 1033
XceI RCATGY 2 cut(s) 696, 700
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 7 cut(s) 323, 713, 870, 1079, 1190, 1311, 1343
ZrmI AGTACT 1 cut(s) 1329
Zsp2I ATGCAT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.