Rh2AG344100

COBRA-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0018533
Physical Location & Seq
Forward (+)
50594577 .. 50596515
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG344100.1

Sequence Viewer

Length: 570 bp
ATGACTGCTTTTGCGGAGGCCTATGATCCATTGGATCCCAATGGAAACATTACTATTCGTTGGGATATAATGAATTGGGCGCCAGATGGATACGAGGCAACCATCACAATGTTTAACTTCCAGAAATATCGGCATATCGAACAGCCAGGTTGGACATTAGGATGGACCTGGGCAAAAAAAGTATTCATATGGTCTATGATAGGAGGGAAAACAATAGACCGAGGGGACTGCTTAAGTTTCACAAGACCCACTCCACACTGTTGCAAGAAGAATCCACAAGTTGTGGACTTACTTCCTGATGTCGGAAGTTGTTGTAGGGGAGGAGTCATGAGCTCGTTGATTCAGGACACGAGCAAAGCTGTCAGCAGATTCCAGATTACTGTGGGTGGTGCCGGAAGTAACAAGAGCACTATCAAAGTCCCCAAGAAATTTACACTGAGAGCACCGGGGCCAGGATACACGTGTGGGGCAGCAGAGATTGTGAAACCAACCATTTTTTACTCACCTGATGGAAGGAGAGCGATGCCAGCCTTCAGTAAGTCGGAAATCCTACTAGGCAGGGATAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

189

Amino Acids

20.94

Weight (kDa)

9.3

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COBRA PF04833 31 - 179 9.5e-51 COBRA-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018533)

Species Orthologous Gene IDs
pyrus_communis pycom06g18180
rosa_chinensis RchiOBHm_Chr2g0130691
rosa_multiflora Rmu_co8433061.1_g000001 Rmu_sc0002279.1_g000054 Rmu_sc0041315.1_g000001
rosa_roxburghii Rroxscaffold_2G00113710
rosa_samantha Rh2AG344100 Rh2CG331300 Rh2DG370600
rosa_wichuraiana Rw2G027550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 79, 389
AciI CCGC 1 cut(s) 14
AclWI GGATC 3 cut(s) 20, 29, 42
AcsI RAATTY 1 cut(s) 428
AcuI CTGAAG 1 cut(s) 517
AcvI CACGTG 1 cut(s) 462
AcyI GRCGYC 1 cut(s) 80
AfiI CCNNNNNNNGG 2 cut(s) 302, 452
AflII CTTAAG 1 cut(s) 232
AflIII ACRYGT 2 cut(s) 459, 461
AjnI CCWGG 3 cut(s) 145, 167, 451
AluBI AGCT 2 cut(s) 333, 359
AluI AGCT 2 cut(s) 333, 359
Alw21I GWGCWC 3 cut(s) 335, 410, 445
AlwI GGATC 3 cut(s) 20, 29, 42
AoxI GGCC 2 cut(s) 18, 449
ApeKI GCWGC 1 cut(s) 470
ApoI RAATTY 1 cut(s) 428
AspLEI GCGC 1 cut(s) 82
AspS9I GGNCC 2 cut(s) 165, 449
AsuC2I CCSGG 1 cut(s) 447
AsuHPI GGTGA 1 cut(s) 495
AvaII GGWCC 1 cut(s) 165
BaeI ACNNNNGTAYC 2 cut(s) 448, 481
BamHI GGATCC 1 cut(s) 34
BanI GGYRCC 2 cut(s) 79, 389
BanII GRGCYC 1 cut(s) 335
BauI CACGAG 1 cut(s) 349
BbrPI CACGTG 1 cut(s) 462
Bbv12I GWGCWC 3 cut(s) 335, 410, 445
BbvI GCAGC 1 cut(s) 482
BccI CCATC 4 cut(s) 80, 110, 156, 503
BcgI CGANNNNNNTGC 2 cut(s) 210, 244
BciT130I CCWGG 3 cut(s) 147, 169, 453
BciVI GTATCC 2 cut(s) 83, 449
BcnI CCSGG 1 cut(s) 447
BfaI CTAG 1 cut(s) 554
BfoI RGCGCY 1 cut(s) 83
BfrI CTTAAG 1 cut(s) 232
BfuI GTATCC 2 cut(s) 83, 449
BisI GCNGC 1 cut(s) 471
BlsI GCNGC 1 cut(s) 472
Bme1390I CCNGG 4 cut(s) 147, 169, 447, 453
Bme18I GGWCC 1 cut(s) 165
BmgT120I GGNCC 2 cut(s) 165, 449
BmiI GGNNCC 4 cut(s) 36, 81, 391, 450
BmrFI CCNGG 4 cut(s) 147, 169, 447, 453
BmsI GCATC 1 cut(s) 513
BpuMI CCSGG 1 cut(s) 447
BsaAI YACGTR 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 168, 220, 446
Bsc4I CCNNNNNNNGG 2 cut(s) 302, 452
BseBI CCWGG 3 cut(s) 147, 169, 453
BseDI CCNNGG 3 cut(s) 168, 220, 446
BseGI GGATG 1 cut(s) 167
BseLI CCNNNNNNNGG 2 cut(s) 302, 452
BseMII CTCAG 1 cut(s) 428
BseRI GAGGAG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 482
BshFI GGCC 2 cut(s) 20, 451
BshNI GGYRCC 2 cut(s) 79, 389
BsiHKAI GWGCWC 3 cut(s) 335, 410, 445
BsiSI CCGG 2 cut(s) 393, 446
BslFI GGGAC 2 cut(s) 239, 404
BslI CCNNNNNNNGG 2 cut(s) 302, 452
BsmFI GGGAC 2 cut(s) 239, 404
BsnI GGCC 2 cut(s) 20, 451
Bsp1286I GDGCHC 3 cut(s) 335, 410, 445
Bsp143I GATC 2 cut(s) 25, 34
BspACI CCGC 1 cut(s) 14
BspANI GGCC 2 cut(s) 20, 451
BspCNI CTCAG 1 cut(s) 429
BspHI TCATGA 1 cut(s) 327
BspLI GGNNCC 4 cut(s) 36, 81, 391, 450
BspPI GGATC 3 cut(s) 20, 29, 42
BspT107I GGYRCC 2 cut(s) 79, 389
BspTI CTTAAG 1 cut(s) 232
BssECI CCNNGG 3 cut(s) 168, 220, 446
BssMI GATC 2 cut(s) 25, 34
BssNI GRCGYC 1 cut(s) 80
BssSI CACGAG 1 cut(s) 349
Bst2BI CACGAG 1 cut(s) 349
Bst2UI CCWGG 3 cut(s) 147, 169, 453
Bst4CI ACNGT 2 cut(s) 260, 382
BstACI GRCGYC 1 cut(s) 80
BstAFI CTTAAG 1 cut(s) 232
BstBAI YACGTR 1 cut(s) 462
BstC8I GCNNGC 1 cut(s) 528
BstDEI CTNAG 1 cut(s) 437
BstF5I GGATG 1 cut(s) 167
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 2 cut(s) 28, 37
BstMBI GATC 2 cut(s) 25, 34
BstMWI GCNNNNNNNGC 1 cut(s) 527
BstNI CCWGG 3 cut(s) 147, 169, 453
BstSCI CCNGG 4 cut(s) 145, 167, 445, 451
BstV1I GCAGC 1 cut(s) 482
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuI GTATCC 2 cut(s) 83, 449
BsuRI GGCC 2 cut(s) 20, 451
BtgZI GCGATG 1 cut(s) 536
BtsCI GGATG 1 cut(s) 167
BtsIMutI CAGTG 2 cut(s) 256, 434
Cac8I GCNNGC 1 cut(s) 528
CciI TCATGA 1 cut(s) 327
CfoI GCGC 1 cut(s) 82
Cfr13I GGNCC 2 cut(s) 165, 449
CviAII CATG 1 cut(s) 328
CviJI RGCY 6 cut(s) 20, 145, 333, 359, 451, 530
CviKI_1 RGCY 6 cut(s) 20, 145, 333, 359, 451, 530
DdeI CTNAG 1 cut(s) 437
DinI GGCGCC 1 cut(s) 81
DpnI GATC 2 cut(s) 27, 36
DpnII GATC 2 cut(s) 25, 34
Ecl136II GAGCTC 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 165
Eco53kI GAGCTC 1 cut(s) 333
Eco57I CTGAAG 1 cut(s) 517
Eco72I CACGTG 1 cut(s) 462
EcoICRI GAGCTC 1 cut(s) 333
EcoRII CCWGG 3 cut(s) 145, 167, 451
EcoT38I GRGCYC 1 cut(s) 335
EgeI GGCGCC 1 cut(s) 81
EheI GGCGCC 1 cut(s) 81
FaeI CATG 1 cut(s) 331
FaiI YATR 7 cut(s) 24, 68, 135, 188, 190, 197, 329
FaqI GGGAC 2 cut(s) 239, 404
FatI CATG 1 cut(s) 327
FauNDI CATATG 1 cut(s) 188
Fnu4HI GCNGC 1 cut(s) 471
FokI GGATG 1 cut(s) 174
FriOI GRGCYC 1 cut(s) 335
Fsp4HI GCNGC 1 cut(s) 471
FspBI CTAG 1 cut(s) 554
GlaI GCGC 1 cut(s) 81
GluI GCNGC 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 2 cut(s) 20, 451
HapII CCGG 2 cut(s) 393, 446
HhaI GCGC 1 cut(s) 82
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 1 cut(s) 331
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HinfI GANTC 4 cut(s) 271, 324, 340, 369
HpaII CCGG 2 cut(s) 393, 446
HphI GGTGA 1 cut(s) 495
Hpy166II GTNNAC 1 cut(s) 286
Hpy188I TCNGA 2 cut(s) 305, 544
Hpy188III TCNNGA 5 cut(s) 121, 296, 328, 344, 373
Hpy8I GTNNAC 1 cut(s) 286
HpyAV CCTTC 2 cut(s) 507, 541
HpyCH4III ACNGT 2 cut(s) 260, 382
HpyCH4IV ACGT 1 cut(s) 461
HpyCH4V TGCA 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 1 cut(s) 527
HpyF3I CTNAG 1 cut(s) 437
HpySE526I ACGT 1 cut(s) 461
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 1 cut(s) 331
HspAI GCGC 1 cut(s) 80
KasI GGCGCC 1 cut(s) 79
Kzo9I GATC 2 cut(s) 25, 34
Lsp1109I GCAGC 1 cut(s) 482
LweI GCATC 1 cut(s) 513
MaeI CTAG 1 cut(s) 554
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 1 cut(s) 398
MalI GATC 2 cut(s) 27, 36
MboI GATC 2 cut(s) 25, 34
MboII GAAGA 1 cut(s) 280
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 3 cut(s) 335, 410, 445
MluCI AATT 2 cut(s) 73, 428
Mly113I GGCGCC 1 cut(s) 80
MlyI GAGTC 1 cut(s) 333
MmeI TCCRAC 3 cut(s) 131, 283, 522
MnlI CCTC 5 cut(s) 10, 88, 197, 215, 314
MseI TTAA 2 cut(s) 114, 233
MslI CAYNNNNRTG 2 cut(s) 107, 160
MspCI CTTAAG 1 cut(s) 232
MspI CCGG 2 cut(s) 393, 446
MspR9I CCNGG 4 cut(s) 147, 169, 447, 453
MvaI CCWGG 3 cut(s) 147, 169, 453
MwoI GCNNNNNNNGC 1 cut(s) 527
NarI GGCGCC 1 cut(s) 80
NciI CCSGG 1 cut(s) 447
NdeI CATATG 1 cut(s) 188
NdeII GATC 2 cut(s) 25, 34
NlaIII CATG 1 cut(s) 331
NlaIV GGNNCC 4 cut(s) 36, 81, 391, 450
PagI TCATGA 1 cut(s) 327
PceI AGGCCT 1 cut(s) 20
PfeI GAWTC 3 cut(s) 271, 340, 369
PkrI GCNGC 1 cut(s) 472
PleI GAGTC 1 cut(s) 332
PluTI GGCGCC 1 cut(s) 83
PmaCI CACGTG 1 cut(s) 462
PmlI CACGTG 1 cut(s) 462
PpsI GAGTC 1 cut(s) 332
Ppu21I YACGTR 1 cut(s) 462
Psp124BI GAGCTC 1 cut(s) 335
Psp6I CCWGG 3 cut(s) 145, 167, 451
PspCI CACGTG 1 cut(s) 462
PspGI CCWGG 3 cut(s) 145, 167, 451
PspN4I GGNNCC 4 cut(s) 36, 81, 391, 450
PspPI GGNCC 2 cut(s) 165, 449
PsuI RGATCY 1 cut(s) 34
RseI CAYNNNNRTG 2 cut(s) 107, 160
SacI GAGCTC 1 cut(s) 335
SaqAI TTAA 2 cut(s) 114, 233
SatI GCNGC 1 cut(s) 471
Sau3AI GATC 2 cut(s) 25, 34
Sau96I GGNCC 2 cut(s) 165, 449
SchI GAGTC 1 cut(s) 333
ScrFI CCNGG 4 cut(s) 147, 169, 447, 453
SduI GDGCHC 3 cut(s) 335, 410, 445
SetI ASST 7 cut(s) 151, 170, 335, 361, 464, 508, 569
SfaNI GCATC 1 cut(s) 513
SfoI GGCGCC 1 cut(s) 81
SinI GGWCC 1 cut(s) 165
SmiMI CAYNNNNRTG 2 cut(s) 107, 160
SmlI CTYRAG 1 cut(s) 232
SmoI CTYRAG 1 cut(s) 232
Sse9I AATT 2 cut(s) 73, 428
SseBI AGGCCT 1 cut(s) 20
SsiI CCGC 1 cut(s) 14
SspDI GGCGCC 1 cut(s) 79
SspMI CTAG 1 cut(s) 554
SstI GAGCTC 1 cut(s) 335
StuI AGGCCT 1 cut(s) 20
StyD4I CCNGG 4 cut(s) 145, 167, 445, 451
TaaI ACNGT 2 cut(s) 260, 382
TaiI ACGT 1 cut(s) 464
TaqI TCGA 1 cut(s) 138
TaqII GACCGA 1 cut(s) 234
TasI AATT 2 cut(s) 73, 428
TfiI GAWTC 3 cut(s) 271, 340, 369
Tru1I TTAA 2 cut(s) 114, 233
Tru9I TTAA 2 cut(s) 114, 233
TscAI CASTG 2 cut(s) 263, 441
TseI GCWGC 1 cut(s) 470
TspDTI ATGAA 2 cut(s) 86, 175
TspRI CASTG 2 cut(s) 263, 441
Vha464I CTTAAG 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 165
XapI RAATTY 1 cut(s) 428
XspI CTAG 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.