Rroxscaffold_2G00113710

COBRA-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018533
Physical Location & Seq
Reverse (-)
39454535 .. 39459244
4710 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00113710.1

Sequence Viewer

Length: 1152 bp
ATGTCTCTGCTTTCATTCAATATCAAAATCCTACTTGTGCTCAGCTATGTCATTTCAACAGAGGCCTATGATCCATTGGATCCCAATGGAAACATTACTATTCGTTGGGATATAATGAATTGGGCGCCAGATGGATACGAGGCAACCATCACAATGTTTAACTTCCAGAAATATCGGCATATCGAACAGCCAGGTTGGACATTAGGATGGACCTGGGCAAAAAAAGAATTCATATGGTCTATGCTAGGAGGGAAAACAATAGACCGAGGGGACTGCTCAAGTTTCACAGGACCCACTCCACACTGTTGCAAGAAGAATCCACAAGTTGTGGACTTACTTCCTGATGTTCCTATTGATGAGCAGGTCGGAAGTTGTTGTAAGGGAGGAGTCATGAGCTCGTTGATTCAGGACACGAGCAAAGCTGTCAGCAGATTCCAGATTACTGTGGGTGGTGCCGGAAGTGACAAGAGCACTATCAAAGTCCCCAAGAAATTTACACTAAGAGCACCGGGGCTAGGATACACGTGTGGGGCAGCAAAGATTGTGAAACCAACCATTTTTTACTCATCTGACGGAAGGAGAGCGATGCCAGCCTTTAAAACTTGGAATGTAACGTGCATGTACTCTCCGTTCCTAGCTACAGAGTTCCCCACTTGTTGTGTGTCATTAAGTGCATTTTATGATGACAAAATAGTAGACTGCCCAAAGTGTGCTTGTGGTTGTCAGAGAGCAAAAGTTGGTAGTTGTGTAGAGTCAAGCTCTCATTACTCAGGATCAGTTCCTGAAGAATCTAGTATCTCTCCCCTCATCCAGTGTTCGAATCATATGTGTCCGGTGCGTGTTCACTGGCACATTAAACAGAATTACAGGGGCTATTGGAGGGTACAAATTACAATAACCAACTTCAACCTCAACAAGAACTACTCTGAATGGAATGCTGTCGTCCAACATCCAAATTTTAATAATTTGACACGCATTTTCTCTTTCAAGTACTATCCACTAGCCCCTTATGATGGATTCAATGACACTGCCATGCTGTGGGGACTTCCTTACTTCAATGATATTCTCCTACAAGCTGGCCCTCAAGGATACGTGCAGTCGGAAATCCTACTAGGCAGGGATAGATCTTCAACTGACGACCAGGATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

383

Amino Acids

42.99

Weight (kDa)

8.07

Isoelectric Point (pI)

46.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COBRA PF04833 46 - 209 4.6e-61 COBRA-like protein
COB_C PF25079 218 - 375 6.2e-36 COBRA family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018533)

Species Orthologous Gene IDs
pyrus_communis pycom06g18180
rosa_chinensis RchiOBHm_Chr2g0130691
rosa_multiflora Rmu_co8433061.1_g000001 Rmu_sc0002279.1_g000054 Rmu_sc0041315.1_g000001
rosa_roxburghii Rroxscaffold_2G00113710
rosa_samantha Rh2AG344100 Rh2CG331300 Rh2DG370600
rosa_wichuraiana Rw2G027550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 352
AccB1I GGYRCC 2 cut(s) 124, 452
AccB7I CCANNNNNTGG 1 cut(s) 1038
AccI GTMKAC 1 cut(s) 696
AclWI GGATC 4 cut(s) 65, 74, 87, 781
AcsI RAATTY 3 cut(s) 227, 491, 955
AcuI CTGAAG 1 cut(s) 804
AcvI CACGTG 1 cut(s) 525
AcyI GRCGYC 1 cut(s) 125
AfaI GTAC 3 cut(s) 623, 885, 992
AfiI CCNNNNNNNGG 3 cut(s) 515, 1013, 1038
AflIII ACRYGT 2 cut(s) 522, 524
AgsI TTSAA 7 cut(s) 19, 57, 907, 988, 1021, 1057, 1131
AjnI CCWGG 3 cut(s) 190, 212, 1140
AluBI AGCT 6 cut(s) 45, 396, 422, 638, 759, 1076
AluI AGCT 6 cut(s) 45, 396, 422, 638, 759, 1076
Alw21I GWGCWC 4 cut(s) 42, 398, 473, 508
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 4 cut(s) 65, 74, 87, 781
AlwNI CAGNNNCTG 1 cut(s) 782
AoxI GGCC 2 cut(s) 63, 1078
ApeKI GCWGC 1 cut(s) 533
ApoI RAATTY 3 cut(s) 227, 491, 955
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 3 cut(s) 210, 290, 1079
AsuC2I CCSGG 1 cut(s) 510
AsuII TTCGAA 1 cut(s) 818
AvaII GGWCC 2 cut(s) 210, 290
BaeI ACNNNNGTAYC 2 cut(s) 511, 544
BamHI GGATCC 1 cut(s) 79
BanI GGYRCC 2 cut(s) 124, 452
BanII GRGCYC 1 cut(s) 398
BauI CACGAG 1 cut(s) 412
BbrPI CACGTG 1 cut(s) 525
Bbv12I GWGCWC 4 cut(s) 42, 398, 473, 508
BbvI GCAGC 1 cut(s) 545
BccI CCATC 4 cut(s) 125, 155, 201, 1007
BcgI CGANNNNNNTGC 2 cut(s) 255, 289
BciT130I CCWGG 3 cut(s) 192, 214, 1142
BciVI GTATCC 3 cut(s) 128, 512, 1082
BcnI CCSGG 1 cut(s) 510
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 6 cut(s) 245, 515, 635, 792, 1001, 1112
BfmI CTRYAG 1 cut(s) 639
BfoI RGCGCY 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 352
BfuI GTATCC 3 cut(s) 128, 512, 1082
BglII AGATCT 1 cut(s) 1124
BisI GCNGC 1 cut(s) 534
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 1 cut(s) 535
BmcAI AGTACT 1 cut(s) 992
Bme1390I CCNGG 4 cut(s) 192, 214, 510, 1142
Bme18I GGWCC 2 cut(s) 210, 290
BmgT120I GGNCC 3 cut(s) 210, 290, 1079
BmiI GGNNCC 4 cut(s) 81, 126, 292, 454
BmrFI CCNGG 4 cut(s) 192, 214, 510, 1142
BmsI GCATC 1 cut(s) 576
BplI GAGNNNNNCTC 2 cut(s) 743, 775
Bpu1102I GCTNAGC 1 cut(s) 41
Bpu14I TTCGAA 1 cut(s) 818
BpuEI CTTGAG 2 cut(s) 262, 1068
BpuMI CCSGG 1 cut(s) 510
BsaAI YACGTR 2 cut(s) 525, 1093
BsaHI GRCGYC 1 cut(s) 125
BsaJI CCNNGG 3 cut(s) 213, 265, 509
BsaWI WCCGGW 1 cut(s) 832
Bsc4I CCNNNNNNNGG 3 cut(s) 515, 1013, 1038
Bse1I ACTGG 2 cut(s) 811, 851
BseBI CCWGG 3 cut(s) 192, 214, 1142
BseDI CCNNGG 3 cut(s) 213, 265, 509
BseGI GGATG 3 cut(s) 212, 807, 949
BseLI CCNNNNNNNGG 3 cut(s) 515, 1013, 1038
BseMII CTCAG 2 cut(s) 55, 783
BseNI ACTGG 2 cut(s) 811, 851
BseRI GAGGAG 1 cut(s) 399
BseXI GCAGC 1 cut(s) 545
BsgI GTGCAG 1 cut(s) 1115
BshFI GGCC 2 cut(s) 65, 1080
BshNI GGYRCC 2 cut(s) 124, 452
BsiHKAI GWGCWC 4 cut(s) 42, 398, 473, 508
BsiSI CCGG 3 cut(s) 456, 509, 833
BslFI GGGAC 3 cut(s) 284, 467, 1056
BslI CCNNNNNNNGG 3 cut(s) 515, 1013, 1038
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 3 cut(s) 284, 467, 1056
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 2 cut(s) 65, 1080
Bsp119I TTCGAA 1 cut(s) 818
Bsp1286I GDGCHC 4 cut(s) 42, 398, 473, 508
Bsp143I GATC 4 cut(s) 70, 79, 773, 1124
Bsp1720I GCTNAGC 1 cut(s) 41
BspANI GGCC 2 cut(s) 65, 1080
BspCNI CTCAG 2 cut(s) 54, 782
BspHI TCATGA 1 cut(s) 390
BspLI GGNNCC 4 cut(s) 81, 126, 292, 454
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 4 cut(s) 65, 74, 87, 781
BspT104I TTCGAA 1 cut(s) 818
BspT107I GGYRCC 2 cut(s) 124, 452
BsrI ACTGG 2 cut(s) 811, 851
BssECI CCNNGG 3 cut(s) 213, 265, 509
BssMI GATC 4 cut(s) 70, 79, 773, 1124
BssNI GRCGYC 1 cut(s) 125
BssSI CACGAG 1 cut(s) 412
Bst2BI CACGAG 1 cut(s) 412
Bst2UI CCWGG 3 cut(s) 192, 214, 1142
Bst4CI ACNGT 2 cut(s) 305, 445
BstACI GRCGYC 1 cut(s) 125
BstBAI YACGTR 2 cut(s) 525, 1093
BstBI TTCGAA 1 cut(s) 818
BstC8I GCNNGC 2 cut(s) 591, 1078
BstDEI CTNAG 3 cut(s) 41, 500, 769
BstF5I GGATG 3 cut(s) 212, 807, 949
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 4 cut(s) 73, 82, 776, 1127
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 70, 79, 773, 1124
BstMWI GCNNNNNNNGC 1 cut(s) 590
BstNI CCWGG 3 cut(s) 192, 214, 1142
BstNSI RCATGY 1 cut(s) 622
BstSCI CCNGG 4 cut(s) 190, 212, 508, 1140
BstSFI CTRYAG 1 cut(s) 639
BstV1I GCAGC 1 cut(s) 545
BstX2I RGATCY 2 cut(s) 79, 1124
BstYI RGATCY 2 cut(s) 79, 1124
BsuI GTATCC 3 cut(s) 128, 512, 1082
BsuRI GGCC 2 cut(s) 65, 1080
BtgZI GCGATG 1 cut(s) 599
BtsCI GGATG 3 cut(s) 212, 807, 949
BtsI GCAGTG 1 cut(s) 1026
BtsIMutI CAGTG 4 cut(s) 301, 818, 844, 1026
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 2 cut(s) 591, 1078
CaiI CAGNNNCTG 1 cut(s) 782
CciI TCATGA 1 cut(s) 390
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 3 cut(s) 210, 290, 1079
Csp6I GTAC 3 cut(s) 622, 884, 991
CviAII CATG 3 cut(s) 391, 619, 1033
CviQI GTAC 3 cut(s) 622, 884, 991
DdeI CTNAG 3 cut(s) 41, 500, 769
DinI GGCGCC 1 cut(s) 126
DpnI GATC 4 cut(s) 72, 81, 775, 1126
DpnII GATC 4 cut(s) 70, 79, 773, 1124
DraI TTTAAA 1 cut(s) 598
Ecl136II GAGCTC 1 cut(s) 396
Eco147I AGGCCT 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 398
Eco47I GGWCC 2 cut(s) 210, 290
Eco53kI GAGCTC 1 cut(s) 396
Eco57I CTGAAG 1 cut(s) 804
Eco72I CACGTG 1 cut(s) 525
EcoICRI GAGCTC 1 cut(s) 396
EcoO109I RGGNCCY 1 cut(s) 290
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 3 cut(s) 190, 212, 1140
EcoT38I GRGCYC 1 cut(s) 398
EgeI GGCGCC 1 cut(s) 126
EheI GGCGCC 1 cut(s) 126
FaeI CATG 3 cut(s) 394, 622, 1036
FaqI GGGAC 3 cut(s) 284, 467, 1056
FatI CATG 3 cut(s) 390, 618, 1032
FauNDI CATATG 2 cut(s) 233, 825
FblI GTMKAC 1 cut(s) 696
Fnu4HI GCNGC 1 cut(s) 534
FokI GGATG 3 cut(s) 219, 794, 936
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 1 cut(s) 534
FspBI CTAG 6 cut(s) 245, 515, 635, 792, 1001, 1112
GlaI GCGC 1 cut(s) 126
GluI GCNGC 1 cut(s) 534
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 2 cut(s) 65, 1080
HapII CCGG 3 cut(s) 456, 509, 833
HhaI GCGC 1 cut(s) 127
Hin1I GRCGYC 1 cut(s) 125
Hin1II CATG 3 cut(s) 394, 622, 1036
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HinfI GANTC 8 cut(s) 316, 387, 403, 432, 752, 788, 820, 1017
HpaII CCGG 3 cut(s) 456, 509, 833
Hpy166II GTNNAC 3 cut(s) 331, 697, 844
Hpy188I TCNGA 5 cut(s) 368, 571, 726, 928, 1102
Hpy188III TCNNGA 7 cut(s) 166, 341, 391, 407, 436, 771, 782
Hpy8I GTNNAC 3 cut(s) 331, 697, 844
HpyAV CCTTC 1 cut(s) 570
HpyCH4III ACNGT 2 cut(s) 305, 445
HpyCH4IV ACGT 3 cut(s) 524, 614, 1092
HpyCH4V TGCA 4 cut(s) 309, 618, 674, 1096
HpyF10VI GCNNNNNNNGC 1 cut(s) 590
HpyF3I CTNAG 3 cut(s) 41, 500, 769
HpySE526I ACGT 3 cut(s) 524, 614, 1092
Hsp92I GRCGYC 1 cut(s) 125
Hsp92II CATG 3 cut(s) 394, 622, 1036
HspAI GCGC 1 cut(s) 125
KasI GGCGCC 1 cut(s) 124
Kzo9I GATC 4 cut(s) 70, 79, 773, 1124
Lsp1109I GCAGC 1 cut(s) 545
LweI GCATC 1 cut(s) 576
MaeI CTAG 6 cut(s) 245, 515, 635, 792, 1001, 1112
MaeII ACGT 3 cut(s) 524, 614, 1092
MaeIII GTNAC 2 cut(s) 461, 610
MalI GATC 4 cut(s) 72, 81, 775, 1126
MboI GATC 4 cut(s) 70, 79, 773, 1124
MboII GAAGA 3 cut(s) 325, 797, 1119
MflI RGATCY 2 cut(s) 79, 1124
MhlI GDGCHC 4 cut(s) 42, 398, 473, 508
MluCI AATT 7 cut(s) 118, 227, 491, 862, 888, 955, 964
Mly113I GGCGCC 1 cut(s) 125
MlyI GAGTC 2 cut(s) 396, 761
MmeI TCCRAC 4 cut(s) 176, 346, 970, 1080
MnlI CCTC 9 cut(s) 55, 133, 242, 260, 377, 815, 873, 920, 1092
MseI TTAA 5 cut(s) 159, 597, 668, 855, 960
MslI CAYNNNNRTG 3 cut(s) 152, 205, 1031
MspI CCGG 3 cut(s) 456, 509, 833
MspR9I CCNGG 4 cut(s) 192, 214, 510, 1142
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 3 cut(s) 192, 214, 1142
MwoI GCNNNNNNNGC 1 cut(s) 590
NarI GGCGCC 1 cut(s) 125
NciI CCSGG 1 cut(s) 510
NdeI CATATG 2 cut(s) 233, 825
NdeII GATC 4 cut(s) 70, 79, 773, 1124
NlaIII CATG 3 cut(s) 394, 622, 1036
NlaIV GGNNCC 4 cut(s) 81, 126, 292, 454
NmuCI GTSAC 1 cut(s) 461
NspI RCATGY 1 cut(s) 622
NspV TTCGAA 1 cut(s) 818
PagI TCATGA 1 cut(s) 390
PceI AGGCCT 1 cut(s) 65
PctI GAATGC 1 cut(s) 940
PfeI GAWTC 6 cut(s) 316, 403, 432, 788, 820, 1017
PflMI CCANNNNNTGG 1 cut(s) 1038
PkrI GCNGC 1 cut(s) 535
PleI GAGTC 2 cut(s) 395, 760
PluTI GGCGCC 1 cut(s) 128
PmaCI CACGTG 1 cut(s) 525
PmlI CACGTG 1 cut(s) 525
PpsI GAGTC 2 cut(s) 395, 760
Ppu21I YACGTR 2 cut(s) 525, 1093
PpuMI RGGWCCY 1 cut(s) 290
Psp124BI GAGCTC 1 cut(s) 398
Psp5II RGGWCCY 1 cut(s) 290
Psp6I CCWGG 3 cut(s) 190, 212, 1140
PspCI CACGTG 1 cut(s) 525
PspGI CCWGG 3 cut(s) 190, 212, 1140
PspN4I GGNNCC 4 cut(s) 81, 126, 292, 454
PspPI GGNCC 3 cut(s) 210, 290, 1079
PspPPI RGGWCCY 1 cut(s) 290
PsrI GAACNNNNNNTAC 2 cut(s) 614, 646
PstNI CAGNNNCTG 1 cut(s) 782
PsuI RGATCY 2 cut(s) 79, 1124
RsaI GTAC 3 cut(s) 623, 885, 992
RsaNI GTAC 3 cut(s) 622, 884, 991
RseI CAYNNNNRTG 3 cut(s) 152, 205, 1031
SacI GAGCTC 1 cut(s) 398
SaqAI TTAA 5 cut(s) 159, 597, 668, 855, 960
SatI GCNGC 1 cut(s) 534
Sau3AI GATC 4 cut(s) 70, 79, 773, 1124
Sau96I GGNCC 3 cut(s) 210, 290, 1079
ScaI AGTACT 1 cut(s) 992
SchI GAGTC 2 cut(s) 396, 761
ScrFI CCNGG 4 cut(s) 192, 214, 510, 1142
SduI GDGCHC 4 cut(s) 42, 398, 473, 508
SfaNI GCATC 1 cut(s) 576
SfcI CTRYAG 1 cut(s) 639
SfoI GGCGCC 1 cut(s) 126
SfuI TTCGAA 1 cut(s) 818
SinI GGWCC 2 cut(s) 210, 290
SmiMI CAYNNNNRTG 3 cut(s) 152, 205, 1031
SmlI CTYRAG 2 cut(s) 277, 1083
SmoI CTYRAG 2 cut(s) 277, 1083
Sse9I AATT 7 cut(s) 118, 227, 491, 862, 888, 955, 964
SseBI AGGCCT 1 cut(s) 65
SspDI GGCGCC 1 cut(s) 124
SspMI CTAG 6 cut(s) 245, 515, 635, 792, 1001, 1112
SstI GAGCTC 1 cut(s) 398
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 4 cut(s) 190, 212, 508, 1140
TaaI ACNGT 2 cut(s) 305, 445
TaiI ACGT 3 cut(s) 527, 617, 1095
TaqI TCGA 2 cut(s) 183, 818
TaqII GACCGA 1 cut(s) 279
TasI AATT 7 cut(s) 118, 227, 491, 862, 888, 955, 964
TatI WGTACW 2 cut(s) 621, 990
TfiI GAWTC 6 cut(s) 316, 403, 432, 788, 820, 1017
Tru1I TTAA 5 cut(s) 159, 597, 668, 855, 960
Tru9I TTAA 5 cut(s) 159, 597, 668, 855, 960
TscAI CASTG 4 cut(s) 308, 818, 851, 1033
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 1 cut(s) 533
Tsp45I GTSAC 1 cut(s) 461
TspDTI ATGAA 2 cut(s) 131, 220
TspGWI ACGGA 2 cut(s) 588, 618
TspRI CASTG 4 cut(s) 308, 818, 851, 1033
Van91I CCANNNNNTGG 1 cut(s) 1038
VpaK11BI GGWCC 2 cut(s) 210, 290
XapI RAATTY 3 cut(s) 227, 491, 955
XceI RCATGY 1 cut(s) 622
XmiI GTMKAC 1 cut(s) 696
XspI CTAG 6 cut(s) 245, 515, 635, 792, 1001, 1112
ZrmI AGTACT 1 cut(s) 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.