Rh2CG331300

COBRA-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0018533
Physical Location & Seq
Forward (+)
43333701 .. 43336792
3092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG331300.1

Sequence Viewer

Length: 1281 bp
ATGACTGCTTTTGCAGAGGCCTATGATCCATTGGATCCCAATGGAAACATTACTATTCGTTGGGATATAATGAATTGGGCGCCAGATGGATACGAGGCAACCATCACAATGTTTAACTTCCAGAAATATCGGCATATCGAACAGCCAGGTTGGACATTAGGATGGACCTGGGCAAAAAAAGAATTCATATGGTCTATGCTAGGAGGGAAAACAATAGACCGAGGGGACTGCTCAAGTTTCACAGGACCCACTCCACACTGTTGCAAGAAGAATCCACAAGTTGTGGACTTACTTCCTGATGTGCCTATTGATGAGCAGGTCGGAAGTTGTTGTAGGGGAGGAGTCATGAGCTCGTTGATTCAGGACACGAGCAAAGCTGTCAGCAGATTCCAGATTACTGTGGGTGGTGCCGGAAGTAACAAGAGCACTATCAAAGTCCCCAAGAAATTTACACTGAGAGCACCGGGGCCAGGATACACGTGTGGGGCAGCAGAGATTGTGAAACCAACCATTTTTTACTCATCTGATGGAAGGAGAGCGCTGCCAGCCTTCAAAACTTGGAATGTAACATGCATGTACTCTCCGTTCCTAGCTACAGAGTTCCCCACCTGTTGTGTGTCATTAAGTGCATTTTATGATGACAAAATAGTAGACTGCCCAAAGTGTGCTTGTGGTTGTCAGAGAGCAAAAGTTGGTAGTTGTGTAGAGTCAAGCTCTCCTTACTCAGGATCAGTTCCTGAAGAATCTATTATCTCTCCCCTCATCCAGTGTTCGAATCATATGTGTCCGGTGCGTGTTCACTGGCACATTAAACAGAATTACAGGGGCTATTGGAGGGTACAAATTACAATAACCAACTTCAACCTCAACAAGAACTACTCTGAATGGAATGCTGTCGTCCAACATCCAAATTTTAATAATTTGACACGCATTTTCTCTTTCAATTACTATCCACTAGCCCCTTATGATGGATTCAATGACACTGCCATGCTGTGGGGACTTCCTTACTTCAATGATATTCTCCTACAAGCTGGCCCTCAAGGATACGTGCAGTCGGAAATCCTAGCAGGCAGGGATAGGTCTGCTTTTACTTGGGAAAATGGTTGGGCTTTCCCTCGGAGGATCTATTTCAATGGTGATATGTGTGTAATGCCAGACGCATATCCTCAATTACCGGCTCCAACTCCTAGATCAAATCTTCCGGTACTTGATCCAGCACTAGTCATTATGGTGGTTGTAATTTGTTATATATTGGTAGGGGCCTTTTGTAAGATTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

426

Amino Acids

47.71

Weight (kDa)

8.01

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COBRA PF04833 31 - 194 3.5e-62 COBRA-like protein
COB_C PF25079 203 - 389 2.2e-48 COBRA family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018533)

Species Orthologous Gene IDs
pyrus_communis pycom06g18180
rosa_chinensis RchiOBHm_Chr2g0130691
rosa_multiflora Rmu_co8433061.1_g000001 Rmu_sc0002279.1_g000054 Rmu_sc0041315.1_g000001
rosa_roxburghii Rroxscaffold_2G00113710
rosa_samantha Rh2AG344100 Rh2CG331300 Rh2DG370600
rosa_wichuraiana Rw2G027550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 307
AccB1I GGYRCC 2 cut(s) 79, 407
AccB7I CCANNNNNTGG 1 cut(s) 993
AccI GTMKAC 1 cut(s) 651
AclWI GGATC 6 cut(s) 20, 29, 42, 736, 1130, 1205
AcsI RAATTY 3 cut(s) 182, 446, 910
AcuI CTGAAG 1 cut(s) 759
AcvI CACGTG 1 cut(s) 480
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 3 cut(s) 578, 840, 1206
AfeI AGCGCT 1 cut(s) 540
AfiI CCNNNNNNNGG 4 cut(s) 470, 725, 968, 993
AflIII ACRYGT 2 cut(s) 477, 479
AgsI TTSAA 6 cut(s) 553, 862, 943, 976, 1012, 1132
AhlI ACTAGT 1 cut(s) 1219
AjnI CCWGG 3 cut(s) 145, 167, 469
AluBI AGCT 5 cut(s) 351, 377, 593, 714, 1031
AluI AGCT 5 cut(s) 351, 377, 593, 714, 1031
Alw21I GWGCWC 3 cut(s) 353, 428, 463
AlwI GGATC 6 cut(s) 20, 29, 42, 736, 1130, 1205
AlwNI CAGNNNCTG 1 cut(s) 737
Aor51HI AGCGCT 1 cut(s) 540
AoxI GGCC 4 cut(s) 18, 467, 1033, 1260
ApeKI GCWGC 2 cut(s) 488, 541
ApoI RAATTY 3 cut(s) 182, 446, 910
AspLEI GCGC 2 cut(s) 82, 541
AspS9I GGNCC 5 cut(s) 165, 245, 467, 1034, 1260
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 1 cut(s) 1148
AsuII TTCGAA 1 cut(s) 773
AvaII GGWCC 2 cut(s) 165, 245
BaeI ACNNNNGTAYC 2 cut(s) 466, 499
BamHI GGATCC 1 cut(s) 34
BanI GGYRCC 2 cut(s) 79, 407
BanII GRGCYC 1 cut(s) 353
BauI CACGAG 1 cut(s) 367
BbrPI CACGTG 1 cut(s) 480
Bbv12I GWGCWC 3 cut(s) 353, 428, 463
BbvI GCAGC 2 cut(s) 500, 528
BccI CCATC 5 cut(s) 80, 110, 156, 521, 962
BcgI CGANNNNNNTGC 2 cut(s) 210, 244
BciT130I CCWGG 3 cut(s) 147, 169, 471
BciVI GTATCC 3 cut(s) 83, 467, 1037
BcnI CCSGG 1 cut(s) 465
BcuI ACTAGT 1 cut(s) 1219
BfaI CTAG 6 cut(s) 200, 590, 956, 1064, 1188, 1220
BfmI CTRYAG 1 cut(s) 594
BfoI RGCGCY 2 cut(s) 83, 542
BfuAI ACCTGC 1 cut(s) 307
BfuI GTATCC 3 cut(s) 83, 467, 1037
BisI GCNGC 2 cut(s) 489, 542
BlsI GCNGC 2 cut(s) 490, 543
Bme1390I CCNGG 4 cut(s) 147, 169, 465, 471
Bme18I GGWCC 2 cut(s) 165, 245
BmgT120I GGNCC 5 cut(s) 165, 245, 467, 1034, 1260
BmiI GGNNCC 7 cut(s) 36, 81, 247, 409, 468, 1179, 1261
BmrFI CCNGG 4 cut(s) 147, 169, 465, 471
BplI GAGNNNNNCTC 2 cut(s) 698, 730
Bpu14I TTCGAA 1 cut(s) 773
BpuEI CTTGAG 2 cut(s) 217, 1023
BpuMI CCSGG 1 cut(s) 465
BsaAI YACGTR 2 cut(s) 480, 1048
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 4 cut(s) 168, 220, 464, 1115
BsaWI WCCGGW 2 cut(s) 787, 1201
Bsc4I CCNNNNNNNGG 4 cut(s) 470, 725, 968, 993
Bse118I RCCGGY 1 cut(s) 1174
Bse1I ACTGG 2 cut(s) 766, 806
BseBI CCWGG 3 cut(s) 147, 169, 471
BseDI CCNNGG 4 cut(s) 168, 220, 464, 1115
BseGI GGATG 3 cut(s) 167, 762, 904
BseLI CCNNNNNNNGG 4 cut(s) 470, 725, 968, 993
BseMII CTCAG 2 cut(s) 446, 738
BseNI ACTGG 2 cut(s) 766, 806
BseRI GAGGAG 1 cut(s) 354
BseXI GCAGC 2 cut(s) 500, 528
BsgI GTGCAG 1 cut(s) 1070
BshFI GGCC 4 cut(s) 20, 469, 1035, 1262
BshNI GGYRCC 2 cut(s) 79, 407
BsiHKAI GWGCWC 3 cut(s) 353, 428, 463
BsiSI CCGG 5 cut(s) 411, 464, 788, 1175, 1202
BslFI GGGAC 3 cut(s) 239, 422, 1011
BslI CCNNNNNNNGG 4 cut(s) 470, 725, 968, 993
BsmFI GGGAC 3 cut(s) 239, 422, 1011
BsmI GAATGC 1 cut(s) 895
BsnI GGCC 4 cut(s) 20, 469, 1035, 1262
Bsp119I TTCGAA 1 cut(s) 773
Bsp1286I GDGCHC 3 cut(s) 353, 428, 463
Bsp143I GATC 6 cut(s) 25, 34, 728, 1122, 1190, 1210
BspANI GGCC 4 cut(s) 20, 469, 1035, 1262
BspCNI CTCAG 2 cut(s) 447, 737
BspHI TCATGA 1 cut(s) 345
BspLI GGNNCC 7 cut(s) 36, 81, 247, 409, 468, 1179, 1261
BspMI ACCTGC 1 cut(s) 307
BspPI GGATC 6 cut(s) 20, 29, 42, 736, 1130, 1205
BspT104I TTCGAA 1 cut(s) 773
BspT107I GGYRCC 2 cut(s) 79, 407
BsrFI RCCGGY 1 cut(s) 1174
BsrI ACTGG 2 cut(s) 766, 806
BssAI RCCGGY 1 cut(s) 1174
BssECI CCNNGG 4 cut(s) 168, 220, 464, 1115
BssMI GATC 6 cut(s) 25, 34, 728, 1122, 1190, 1210
BssNI GRCGYC 1 cut(s) 80
BssSI CACGAG 1 cut(s) 367
Bst2BI CACGAG 1 cut(s) 367
Bst2UI CCWGG 3 cut(s) 147, 169, 471
Bst4CI ACNGT 2 cut(s) 260, 400
BstACI GRCGYC 1 cut(s) 80
BstBAI YACGTR 2 cut(s) 480, 1048
BstBI TTCGAA 1 cut(s) 773
BstC8I GCNNGC 3 cut(s) 546, 1033, 1069
BstDEI CTNAG 2 cut(s) 455, 724
BstENI CCTNNNNNAGG 1 cut(s) 723
BstF5I GGATG 3 cut(s) 167, 762, 904
BstH2I RGCGCY 2 cut(s) 83, 542
BstHHI GCGC 2 cut(s) 82, 541
BstKTI GATC 6 cut(s) 28, 37, 731, 1125, 1193, 1213
BstMBI GATC 6 cut(s) 25, 34, 728, 1122, 1190, 1210
BstMWI GCNNNNNNNGC 1 cut(s) 545
BstNI CCWGG 3 cut(s) 147, 169, 471
BstNSI RCATGY 2 cut(s) 573, 577
BstSCI CCNGG 4 cut(s) 145, 167, 463, 469
BstSFI CTRYAG 1 cut(s) 594
BstV1I GCAGC 2 cut(s) 500, 528
BstX2I RGATCY 2 cut(s) 34, 1122
BstYI RGATCY 2 cut(s) 34, 1122
BsuI GTATCC 3 cut(s) 83, 467, 1037
BsuRI GGCC 4 cut(s) 20, 469, 1035, 1262
BtsCI GGATG 3 cut(s) 167, 762, 904
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 5 cut(s) 256, 452, 773, 799, 981
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 3 cut(s) 546, 1033, 1069
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 1 cut(s) 345
CfoI GCGC 2 cut(s) 82, 541
Cfr10I RCCGGY 1 cut(s) 1174
Cfr13I GGNCC 5 cut(s) 165, 245, 467, 1034, 1260
CseI GACGC 1 cut(s) 1166
Csp6I GTAC 3 cut(s) 577, 839, 1205
CviAII CATG 4 cut(s) 346, 570, 574, 988
CviQI GTAC 3 cut(s) 577, 839, 1205
DdeI CTNAG 2 cut(s) 455, 724
DinI GGCGCC 1 cut(s) 81
DpnI GATC 6 cut(s) 27, 36, 730, 1124, 1192, 1212
DpnII GATC 6 cut(s) 25, 34, 728, 1122, 1190, 1210
Ecl136II GAGCTC 1 cut(s) 351
Eco147I AGGCCT 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 353
Eco47I GGWCC 2 cut(s) 165, 245
Eco47III AGCGCT 1 cut(s) 540
Eco53kI GAGCTC 1 cut(s) 351
Eco57I CTGAAG 1 cut(s) 759
Eco72I CACGTG 1 cut(s) 480
EcoICRI GAGCTC 1 cut(s) 351
EcoNI CCTNNNNNAGG 1 cut(s) 723
EcoO109I RGGNCCY 2 cut(s) 245, 1260
EcoRI GAATTC 1 cut(s) 182
EcoRII CCWGG 3 cut(s) 145, 167, 469
EcoT22I ATGCAT 1 cut(s) 575
EcoT38I GRGCYC 1 cut(s) 353
EgeI GGCGCC 1 cut(s) 81
EheI GGCGCC 1 cut(s) 81
FaeI CATG 4 cut(s) 349, 573, 577, 991
FalI AAGNNNNNCTT 2 cut(s) 703, 735
FaqI GGGAC 3 cut(s) 239, 422, 1011
FatI CATG 4 cut(s) 345, 569, 573, 987
FauNDI CATATG 2 cut(s) 188, 780
FblI GTMKAC 1 cut(s) 651
Fnu4HI GCNGC 2 cut(s) 489, 542
FokI GGATG 3 cut(s) 174, 749, 891
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 489, 542
FspBI CTAG 6 cut(s) 200, 590, 956, 1064, 1188, 1220
GlaI GCGC 2 cut(s) 81, 540
GluI GCNGC 2 cut(s) 489, 542
HaeII RGCGCY 2 cut(s) 83, 542
HaeIII GGCC 4 cut(s) 20, 469, 1035, 1262
HapII CCGG 5 cut(s) 411, 464, 788, 1175, 1202
HgaI GACGC 1 cut(s) 1166
HhaI GCGC 2 cut(s) 82, 541
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 4 cut(s) 349, 573, 577, 991
Hin6I GCGC 2 cut(s) 80, 539
HinP1I GCGC 2 cut(s) 80, 539
HinfI GANTC 8 cut(s) 271, 342, 358, 387, 707, 743, 775, 972
HpaII CCGG 5 cut(s) 411, 464, 788, 1175, 1202
HphI GGTGA 1 cut(s) 1148
Hpy166II GTNNAC 3 cut(s) 286, 652, 799
Hpy188I TCNGA 6 cut(s) 323, 526, 681, 883, 1057, 1119
Hpy188III TCNNGA 7 cut(s) 121, 296, 346, 362, 391, 726, 737
Hpy8I GTNNAC 3 cut(s) 286, 652, 799
HpyAV CCTTC 2 cut(s) 525, 559
HpyCH4III ACNGT 2 cut(s) 260, 400
HpyCH4IV ACGT 2 cut(s) 479, 1047
HpyCH4V TGCA 5 cut(s) 14, 264, 573, 629, 1051
HpyF10VI GCNNNNNNNGC 1 cut(s) 545
HpyF3I CTNAG 2 cut(s) 455, 724
HpySE526I ACGT 2 cut(s) 479, 1047
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 4 cut(s) 349, 573, 577, 991
HspAI GCGC 2 cut(s) 80, 539
KasI GGCGCC 1 cut(s) 79
Kzo9I GATC 6 cut(s) 25, 34, 728, 1122, 1190, 1210
LmnI GCTCC 1 cut(s) 1183
Lsp1109I GCAGC 2 cut(s) 500, 528
MaeI CTAG 6 cut(s) 200, 590, 956, 1064, 1188, 1220
MaeII ACGT 2 cut(s) 479, 1047
MaeIII GTNAC 2 cut(s) 416, 565
MalI GATC 6 cut(s) 27, 36, 730, 1124, 1192, 1212
MboI GATC 6 cut(s) 25, 34, 728, 1122, 1190, 1210
MboII GAAGA 3 cut(s) 280, 752, 1190
MflI RGATCY 2 cut(s) 34, 1122
MhlI GDGCHC 3 cut(s) 353, 428, 463
Mly113I GGCGCC 1 cut(s) 80
MlyI GAGTC 2 cut(s) 351, 716
MmeI TCCRAC 5 cut(s) 131, 301, 925, 1035, 1205
Mph1103I ATGCAT 1 cut(s) 575
MseI TTAA 4 cut(s) 114, 623, 810, 915
MslI CAYNNNNRTG 4 cut(s) 107, 160, 986, 1229
MspI CCGG 5 cut(s) 411, 464, 788, 1175, 1202
MspR9I CCNGG 4 cut(s) 147, 169, 465, 471
Mva1269I GAATGC 1 cut(s) 895
MvaI CCWGG 3 cut(s) 147, 169, 471
MwoI GCNNNNNNNGC 1 cut(s) 545
NarI GGCGCC 1 cut(s) 80
NciI CCSGG 1 cut(s) 465
NdeI CATATG 2 cut(s) 188, 780
NdeII GATC 6 cut(s) 25, 34, 728, 1122, 1190, 1210
NlaIII CATG 4 cut(s) 349, 573, 577, 991
NlaIV GGNNCC 7 cut(s) 36, 81, 247, 409, 468, 1179, 1261
NsiI ATGCAT 1 cut(s) 575
NspI RCATGY 2 cut(s) 573, 577
NspV TTCGAA 1 cut(s) 773
PagI TCATGA 1 cut(s) 345
PceI AGGCCT 1 cut(s) 20
PctI GAATGC 1 cut(s) 895
PfeI GAWTC 6 cut(s) 271, 358, 387, 743, 775, 972
PflMI CCANNNNNTGG 1 cut(s) 993
PkrI GCNGC 2 cut(s) 490, 543
PleI GAGTC 2 cut(s) 350, 715
PluTI GGCGCC 1 cut(s) 83
PmaCI CACGTG 1 cut(s) 480
PmlI CACGTG 1 cut(s) 480
PpsI GAGTC 2 cut(s) 350, 715
Ppu21I YACGTR 2 cut(s) 480, 1048
PpuMI RGGWCCY 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 353
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 3 cut(s) 145, 167, 469
PspCI CACGTG 1 cut(s) 480
PspGI CCWGG 3 cut(s) 145, 167, 469
PspN4I GGNNCC 7 cut(s) 36, 81, 247, 409, 468, 1179, 1261
PspPI GGNCC 5 cut(s) 165, 245, 467, 1034, 1260
PspPPI RGGWCCY 1 cut(s) 245
PsrI GAACNNNNNNTAC 2 cut(s) 569, 601
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 2 cut(s) 34, 1122
RsaI GTAC 3 cut(s) 578, 840, 1206
RsaNI GTAC 3 cut(s) 577, 839, 1205
RseI CAYNNNNRTG 4 cut(s) 107, 160, 986, 1229
SacI GAGCTC 1 cut(s) 353
SaqAI TTAA 4 cut(s) 114, 623, 810, 915
SatI GCNGC 2 cut(s) 489, 542
Sau3AI GATC 6 cut(s) 25, 34, 728, 1122, 1190, 1210
Sau96I GGNCC 5 cut(s) 165, 245, 467, 1034, 1260
SchI GAGTC 2 cut(s) 351, 716
ScrFI CCNGG 4 cut(s) 147, 169, 465, 471
SduI GDGCHC 3 cut(s) 353, 428, 463
SfcI CTRYAG 1 cut(s) 594
SfoI GGCGCC 1 cut(s) 81
SfuI TTCGAA 1 cut(s) 773
SinI GGWCC 2 cut(s) 165, 245
SmiMI CAYNNNNRTG 4 cut(s) 107, 160, 986, 1229
SmlI CTYRAG 2 cut(s) 232, 1038
SmoI CTYRAG 2 cut(s) 232, 1038
SpeI ACTAGT 1 cut(s) 1219
SseBI AGGCCT 1 cut(s) 20
SspDI GGCGCC 1 cut(s) 79
SspMI CTAG 6 cut(s) 200, 590, 956, 1064, 1188, 1220
SstI GAGCTC 1 cut(s) 353
StuI AGGCCT 1 cut(s) 20
StyD4I CCNGG 4 cut(s) 145, 167, 463, 469
TaaI ACNGT 2 cut(s) 260, 400
TaiI ACGT 2 cut(s) 482, 1050
TaqI TCGA 2 cut(s) 138, 773
TaqII GACCGA 1 cut(s) 234
TatI WGTACW 1 cut(s) 576
TfiI GAWTC 6 cut(s) 271, 358, 387, 743, 775, 972
Tru1I TTAA 4 cut(s) 114, 623, 810, 915
Tru9I TTAA 4 cut(s) 114, 623, 810, 915
TscAI CASTG 5 cut(s) 263, 459, 773, 806, 988
TseI GCWGC 2 cut(s) 488, 541
TspDTI ATGAA 2 cut(s) 86, 175
TspGWI ACGGA 1 cut(s) 573
TspRI CASTG 5 cut(s) 263, 459, 773, 806, 988
Van91I CCANNNNNTGG 1 cut(s) 993
VpaK11BI GGWCC 2 cut(s) 165, 245
XagI CCTNNNNNAGG 1 cut(s) 723
XapI RAATTY 3 cut(s) 182, 446, 910
XceI RCATGY 2 cut(s) 573, 577
XmiI GTMKAC 1 cut(s) 651
XspI CTAG 6 cut(s) 200, 590, 956, 1064, 1188, 1220
Zsp2I ATGCAT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.