pycom06g18950

Carbon catabolite repressor protein 4 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0012263
Physical Location & Seq
Reverse (-)
22517703 .. 22521039
3337 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1371 bp
ATGCCGTGCGACGCTTCCTCTTGGCTCTGCGGTGGCCACGTTTCCGTCGCAACAACCGCACCGACTGCCACCACCGCTAGCCCCTCCTCCTCCTTTCTCTGCAAGACCACCGTTTCTTGCTGCACCGCCGCTCCTAAAGGCTCGTCCACCTCCTCATCATCGTCATCTACTGGTTCCCAGACTAGTCGTCCGTGGTACAAGCAGGGACAGTACAGTCATGCTCCGCCGGACGTCGTACGCCGTTGGATCCAAGCAGAAAATCAACCACTCGCTTCTCGAGATAGATTTTCTGTTGCATCATATAACATTTTGGGGGATCGAAATGCTTTCTGGCATAGAGATATGTACCGGAATGTGCCTTCCAAATATCTCAAATGGGACCATCGTAGGAGGGTCATATGCGATGAACTTGCGGAATGGAACGCTGATATAATGTGTTTGCAAGAAGTAGACAAGTATTTTGAGCTCTCGGAGATTCTAGCGAAAGTGGGATATGTCGGATCATACAAGAGACGCACTGGAGGTTCCGTTGATGGTTGCGCTATTTTCTGGAAACCTGATAATTTTCGGTTGTTGGAGGAAGAGAGTATTGAATTCAAGGGCTATGGACTTGGTGACAATGTTGCGCAACTCTCGGTTTTTGAGACGCAAAAAGCTGAATCAAGAAATGTAGTGATCGGTAACATCCACGTGCTTTATAACCCAACCCGAGGGGAAGCAAAATTAGGCCAGATACGTTTTCTCATATCAAGGGCGAAAATCCTCTCAGAGAGATGGGGTAATGCTCCTGTTGTCCTTTGCGGCGATTTCAATATCACTCCTAAGAGTGCAATATACAAGTTCTTGTCAAAATCTGAGCTGAATATCATGTTATATGACAGAAGAGAGTTATCTGGGCAGAGGGATTGTCATCCTGCTCAAGTTTTAGGTGCCAAACAAGAAATTAGCAGTCCACTTACAATGATAGACGGATTGCTGAAGCAATGCTGGACAGATGAAGAAATTAGAGTTGCAACCGGAAATGCCAATTGCCATTTAGTTGTGCATCCGATGAAGCTTAACAGTTCCTATGCCACGGTGCAGGGTTCTACAAGAACACGGGATCCCAATGGTGAACCTGTAGCTACTACCTACCACTCAAAATTTCTTGGAACCGTTGACTACTTATGGTACTCTGATGGACTTGTGCCCACTAGAGTTATAGACACCGTTCCAGTTGATGTTCTGCAGAAGATTGGTAGCCTCCCTTGCAAGAAAGTGGGAAGCGATCATTTGGCCTTAGTTACAGAGTTCGCCTTCAAACCGGGCACAAATGAAGACAACAAAGAAACTGCTGCATCGTCAAGCAGAACAGCGTTTTCCAAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

457

Amino Acids

50.58

Weight (kDa)

8.57

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 99 - 424 1.2e-21 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012263)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18500 AT3G18500 AT3G18500 AT3G18500
fragaria_vesca FvH4_5g12590
malus_domestica MD06G1212300.v1.1
prunus_persica Prupe.5G218400_v2.0.a1
pyrus_communis pycom06g18950
rosa_chinensis RchiOBHm_Chr7g0184741
rosa_laevigata RLG00000004954
rosa_multiflora Rmu_co8486587.1_g000001 Rmu_ssc0000289.1_g000017
rosa_roxburghii Rroxscaffold_3G00269380
rosa_rugosa Rorug06G0464900
rosa_samantha Rh7AG078000 Rh7BG069300 Rh7CG070100 Rh7DG069500
rosa_wichuraiana Rw7G005740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 699
AasI GACNNNNNNGTC 1 cut(s) 213
AatII GACGTC 1 cut(s) 234
Acc16I TGCGCA 1 cut(s) 627
AccB1I GGYRCC 1 cut(s) 929
AccBSI CCGCTC 1 cut(s) 131
AccI GTMKAC 1 cut(s) 450
AciI CCGC 8 cut(s) 30, 57, 75, 126, 129, 224, 413, 801
AclWI GGATC 6 cut(s) 241, 254, 324, 508, 1097, 1110
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 2 cut(s) 593, 1142
AcuI CTGAAG 1 cut(s) 998
AcvI CACGTG 1 cut(s) 691
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 5 cut(s) 197, 212, 237, 347, 1172
AfiI CCNNNNNNNGG 1 cut(s) 710
AgsI TTSAA 4 cut(s) 593, 598, 811, 1300
AhdI GACNNNNNGTC 1 cut(s) 186
AhlI ACTAGT 1 cut(s) 182
AloI GAACNNNNNNTCC 2 cut(s) 1087, 1119
AluBI AGCT 6 cut(s) 466, 656, 859, 1057, 1124, 1366
AluI AGCT 6 cut(s) 466, 656, 859, 1057, 1124, 1366
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 2 cut(s) 505, 638
AlwI GGATC 6 cut(s) 241, 254, 324, 508, 1097, 1110
Ama87I CYCGRG 2 cut(s) 276, 708
AoxI GGCC 3 cut(s) 34, 727, 1275
ApeKI GCWGC 2 cut(s) 120, 1334
ApoI RAATTY 2 cut(s) 593, 1142
ArsI GACNNNNNNTTYG 2 cut(s) 443, 475
AspLEI GCGC 2 cut(s) 542, 628
AspS9I GGNCC 1 cut(s) 379
AsuC2I CCSGG 1 cut(s) 1305
AsuHPI GGTGA 2 cut(s) 626, 1124
AsuNHI GCTAGC 1 cut(s) 77
AvaI CYCGRG 2 cut(s) 276, 708
AvaII GGWCC 1 cut(s) 379
BaeGI GKGCMC 2 cut(s) 1191, 1310
BalI TGGCCA 1 cut(s) 36
BamHI GGATCC 2 cut(s) 246, 1102
BanI GGYRCC 1 cut(s) 929
BanII GRGCYC 1 cut(s) 468
BbrPI CACGTG 1 cut(s) 691
BbsI GAAGAC 1 cut(s) 1323
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 107, 1321
BccI CCATC 4 cut(s) 390, 527, 768, 1172
BceAI ACGGC 1 cut(s) 225
BcgI CGANNNNNNTGC 2 cut(s) 392, 426
BcnI CCSGG 1 cut(s) 1305
BcoDI GTCTC 2 cut(s) 505, 638
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 4 cut(s) 78, 183, 479, 1194
BfmI CTRYAG 2 cut(s) 1119, 1226
BisI GCNGC 4 cut(s) 121, 129, 802, 1335
BlsI GCNGC 4 cut(s) 122, 130, 803, 1336
Bme1390I CCNGG 1 cut(s) 1305
Bme18I GGWCC 1 cut(s) 379
BmeRI GACNNNNNGTC 1 cut(s) 186
BmeT110I CYCGRG 2 cut(s) 276, 708
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 7 cut(s) 175, 248, 380, 526, 931, 1104, 1153
BmrFI CCNGG 1 cut(s) 1305
BmsI GCATC 3 cut(s) 305, 1054, 1346
BmtI GCTAGC 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 1323
BpmI CTGGAG 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 903
BpuMI CCSGG 1 cut(s) 1305
BsaAI YACGTR 1 cut(s) 691
BsaBI GATNNNNATC 1 cut(s) 909
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 191, 709, 1074
BsaWI WCCGGW 2 cut(s) 348, 1016
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 513, 543
Bsc4I CCNNNNNNNGG 1 cut(s) 710
Bse1I ACTGG 3 cut(s) 175, 523, 1214
Bse3DI GCAATG 1 cut(s) 989
Bse8I GATNNNNATC 1 cut(s) 909
BseDI CCNNGG 3 cut(s) 191, 709, 1074
BseGI GGATG 3 cut(s) 684, 910, 1045
BseJI GATNNNNATC 1 cut(s) 909
BseLI CCNNNNNNNGG 1 cut(s) 710
BseMI GCAATG 1 cut(s) 989
BseMII CTCAG 2 cut(s) 780, 846
BseNI ACTGG 3 cut(s) 175, 523, 1214
BseRI GAGGAG 3 cut(s) 76, 79, 142
BseSI GKGCMC 2 cut(s) 1191, 1310
BseXI GCAGC 2 cut(s) 107, 1321
BsgI GTGCAG 2 cut(s) 106, 1100
BshFI GGCC 3 cut(s) 36, 729, 1277
BshNI GGYRCC 1 cut(s) 929
BsiHKAI GWGCWC 1 cut(s) 468
BsiHKCI CYCGRG 2 cut(s) 276, 708
BsiSI CCGG 4 cut(s) 227, 349, 1017, 1304
BsiWI CGTACG 1 cut(s) 235
BslFI GGGAC 2 cut(s) 219, 392
BslI CCNNNNNNNGG 1 cut(s) 710
BsmAI GTCTC 2 cut(s) 505, 638
BsmBI CGTCTC 2 cut(s) 505, 638
BsmFI GGGAC 2 cut(s) 219, 392
BsnI GGCC 3 cut(s) 36, 729, 1277
BsoBI CYCGRG 2 cut(s) 276, 708
Bsp1286I GDGCHC 3 cut(s) 468, 1191, 1310
Bsp143I GATC 6 cut(s) 246, 316, 500, 675, 1102, 1267
BspACI CCGC 8 cut(s) 30, 57, 75, 126, 129, 224, 413, 801
BspANI GGCC 3 cut(s) 36, 729, 1277
BspCNI CTCAG 2 cut(s) 779, 847
BspLI GGNNCC 7 cut(s) 175, 248, 380, 526, 931, 1104, 1153
BspMAI CTGCAG 1 cut(s) 1230
BspOI GCTAGC 1 cut(s) 81
BspPI GGATC 6 cut(s) 241, 254, 324, 508, 1097, 1110
BspT107I GGYRCC 1 cut(s) 929
BsrBI CCGCTC 1 cut(s) 131
BsrDI GCAATG 1 cut(s) 989
BsrI ACTGG 3 cut(s) 175, 523, 1214
BssECI CCNNGG 3 cut(s) 191, 709, 1074
BssMI GATC 6 cut(s) 246, 316, 500, 675, 1102, 1267
BssNI GRCGYC 1 cut(s) 231
Bst4CI ACNGT 7 cut(s) 112, 210, 215, 1064, 1078, 1156, 1210
Bst6I CTCTTC 2 cut(s) 576, 877
BstACI GRCGYC 1 cut(s) 231
BstAPI GCANNNNNTGC 1 cut(s) 65
BstBAI YACGTR 1 cut(s) 691
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 4 cut(s) 766, 822, 855, 1279
BstDSI CCRYGG 2 cut(s) 191, 1074
BstF5I GGATG 3 cut(s) 684, 910, 1045
BstHHI GCGC 2 cut(s) 542, 628
BstKTI GATC 6 cut(s) 249, 319, 503, 678, 1105, 1270
BstMAI GTCTC 2 cut(s) 505, 638
BstMBI GATC 6 cut(s) 246, 316, 500, 675, 1102, 1267
BstMWI GCNNNNNNNGC 4 cut(s) 56, 65, 74, 1248
BstSCI CCNGG 1 cut(s) 1303
BstSFI CTRYAG 2 cut(s) 1119, 1226
BstSLI GKGCMC 2 cut(s) 1191, 1310
BstV1I GCAGC 2 cut(s) 107, 1321
BstV2I GAAGAC 1 cut(s) 1323
BstX2I RGATCY 2 cut(s) 246, 1102
BstYI RGATCY 2 cut(s) 246, 1102
BsuRI GGCC 3 cut(s) 36, 729, 1277
BtgI CCRYGG 2 cut(s) 191, 1074
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 3 cut(s) 684, 910, 1045
BtsIMutI CAGTG 1 cut(s) 516
Cac8I GCNNGC 1 cut(s) 79
CfoI GCGC 2 cut(s) 542, 628
Cfr13I GGNCC 1 cut(s) 379
CseI GACGC 3 cut(s) 20, 522, 655
Csp6I GTAC 5 cut(s) 196, 211, 236, 346, 1171
CviAII CATG 2 cut(s) 218, 868
CviQI GTAC 5 cut(s) 196, 211, 236, 346, 1171
DdeI CTNAG 4 cut(s) 766, 822, 855, 1279
DpnI GATC 6 cut(s) 248, 318, 502, 677, 1104, 1269
DpnII GATC 6 cut(s) 246, 316, 500, 675, 1102, 1267
DrdI GACNNNNNNGTC 1 cut(s) 213
DriI GACNNNNNGTC 1 cut(s) 186
DseDI GACNNNNNNGTC 1 cut(s) 213
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 576, 877
Eam1105I GACNNNNNGTC 1 cut(s) 186
EarI CTCTTC 2 cut(s) 576, 877
EciI GGCGGA 1 cut(s) 213
Ecl136II GAGCTC 1 cut(s) 466
Eco24I GRGCYC 1 cut(s) 468
Eco47I GGWCC 1 cut(s) 379
Eco53kI GAGCTC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 998
Eco72I CACGTG 1 cut(s) 691
Eco88I CYCGRG 2 cut(s) 276, 708
EcoICRI GAGCTC 1 cut(s) 466
EcoRI GAATTC 1 cut(s) 593
EcoT38I GRGCYC 1 cut(s) 468
Esp3I CGTCTC 2 cut(s) 505, 638
FaeI CATG 2 cut(s) 221, 871
FaqI GGGAC 2 cut(s) 219, 392
FatI CATG 2 cut(s) 217, 867
FauNDI CATATG 1 cut(s) 398
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 4 cut(s) 121, 129, 802, 1335
FokI GGATG 3 cut(s) 671, 897, 1032
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 4 cut(s) 121, 129, 802, 1335
FspBI CTAG 4 cut(s) 78, 183, 479, 1194
FspI TGCGCA 1 cut(s) 627
GlaI GCGC 2 cut(s) 541, 627
GluI GCNGC 4 cut(s) 121, 129, 802, 1335
GsuI CTGGAG 1 cut(s) 540
HaeIII GGCC 3 cut(s) 36, 729, 1277
HapII CCGG 4 cut(s) 227, 349, 1017, 1304
HgaI GACGC 3 cut(s) 20, 522, 655
HhaI GCGC 2 cut(s) 542, 628
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 2 cut(s) 221, 871
Hin6I GCGC 2 cut(s) 540, 626
HinP1I GCGC 2 cut(s) 540, 626
HincII GTYRAC 1 cut(s) 1159
HindII GTYRAC 1 cut(s) 1159
HindIII AAGCTT 2 cut(s) 1055, 1364
HinfI GANTC 2 cut(s) 475, 659
HpaII CCGG 4 cut(s) 227, 349, 1017, 1304
HphI GGTGA 2 cut(s) 626, 1124
Hpy166II GTNNAC 5 cut(s) 147, 451, 953, 1115, 1159
Hpy188I TCNGA 6 cut(s) 472, 500, 769, 856, 1050, 1177
Hpy188III TCNNGA 4 cut(s) 276, 278, 550, 663
Hpy8I GTNNAC 5 cut(s) 147, 451, 953, 1115, 1159
Hpy99I CGWCG 3 cut(s) 14, 50, 236
HpyAV CCTTC 2 cut(s) 369, 1306
HpyCH4III ACNGT 7 cut(s) 112, 210, 215, 1064, 1078, 1156, 1210
HpyCH4IV ACGT 4 cut(s) 39, 231, 690, 736
HpyF10VI GCNNNNNNNGC 4 cut(s) 56, 65, 74, 1248
HpyF3I CTNAG 4 cut(s) 766, 822, 855, 1279
HpySE526I ACGT 4 cut(s) 39, 231, 690, 736
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 2 cut(s) 221, 871
HspAI GCGC 2 cut(s) 540, 626
Kzo9I GATC 6 cut(s) 246, 316, 500, 675, 1102, 1267
LmnI GCTCC 3 cut(s) 136, 226, 790
Lsp1109I GCAGC 2 cut(s) 107, 1321
LweI GCATC 3 cut(s) 305, 1054, 1346
MaeI CTAG 4 cut(s) 78, 183, 479, 1194
MaeII ACGT 4 cut(s) 39, 231, 690, 736
MaeIII GTNAC 3 cut(s) 614, 680, 1282
MalI GATC 6 cut(s) 248, 318, 502, 677, 1104, 1269
MbiI CCGCTC 1 cut(s) 131
MboI GATC 6 cut(s) 246, 316, 500, 675, 1102, 1267
MboII GAAGA 5 cut(s) 593, 894, 1010, 1243, 1328
MfeI CAATTG 1 cut(s) 1027
MflI RGATCY 2 cut(s) 246, 1102
MhlI GDGCHC 3 cut(s) 468, 1191, 1310
MlsI TGGCCA 1 cut(s) 36
MluCI AATT 7 cut(s) 562, 593, 722, 942, 1002, 1027, 1142
MluNI TGGCCA 1 cut(s) 36
MmeI TCCRAC 3 cut(s) 224, 478, 555
Mox20I TGGCCA 1 cut(s) 36
MscI TGGCCA 1 cut(s) 36
MseI TTAA 1 cut(s) 1059
MslI CAYNNNNRTG 1 cut(s) 689
Msp20I TGGCCA 1 cut(s) 36
MspI CCGG 4 cut(s) 227, 349, 1017, 1304
MspR9I CCNGG 1 cut(s) 1305
MunI CAATTG 1 cut(s) 1027
MwoI GCNNNNNNNGC 4 cut(s) 56, 65, 74, 1248
NciI CCSGG 1 cut(s) 1305
NdeI CATATG 1 cut(s) 398
NdeII GATC 6 cut(s) 246, 316, 500, 675, 1102, 1267
NheI GCTAGC 1 cut(s) 77
NlaIII CATG 2 cut(s) 221, 871
NlaIV GGNNCC 7 cut(s) 175, 248, 380, 526, 931, 1104, 1153
NmuCI GTSAC 1 cut(s) 614
NsbI TGCGCA 1 cut(s) 627
PaeR7I CTCGAG 1 cut(s) 276
PfeI GAWTC 2 cut(s) 475, 659
Pfl23II CGTACG 1 cut(s) 235
PkrI GCNGC 4 cut(s) 122, 130, 803, 1336
PmaCI CACGTG 1 cut(s) 691
PmlI CACGTG 1 cut(s) 691
Ppu21I YACGTR 1 cut(s) 691
PsiI TTATAA 1 cut(s) 699
Psp124BI GAGCTC 1 cut(s) 468
PspCI CACGTG 1 cut(s) 691
PspLI CGTACG 1 cut(s) 235
PspN4I GGNNCC 7 cut(s) 175, 248, 380, 526, 931, 1104, 1153
PspPI GGNCC 1 cut(s) 379
PstI CTGCAG 1 cut(s) 1230
PsuI RGATCY 2 cut(s) 246, 1102
RsaI GTAC 5 cut(s) 197, 212, 237, 347, 1172
RsaNI GTAC 5 cut(s) 196, 211, 236, 346, 1171
RseI CAYNNNNRTG 1 cut(s) 689
SacI GAGCTC 1 cut(s) 468
SaqAI TTAA 1 cut(s) 1059
SatI GCNGC 4 cut(s) 121, 129, 802, 1335
Sau3AI GATC 6 cut(s) 246, 316, 500, 675, 1102, 1267
Sau96I GGNCC 1 cut(s) 379
ScrFI CCNGG 1 cut(s) 1305
SduI GDGCHC 3 cut(s) 468, 1191, 1310
SfaNI GCATC 3 cut(s) 305, 1054, 1346
SfcI CTRYAG 2 cut(s) 1119, 1226
Sfr274I CTCGAG 1 cut(s) 276
SinI GGWCC 1 cut(s) 379
SlaI CTCGAG 1 cut(s) 276
SmiMI CAYNNNNRTG 1 cut(s) 689
SmlI CTYRAG 2 cut(s) 276, 918
SmoI CTYRAG 2 cut(s) 276, 918
SpeI ACTAGT 1 cut(s) 182
Sse9I AATT 7 cut(s) 562, 593, 722, 942, 1002, 1027, 1142
SsiI CCGC 8 cut(s) 30, 57, 75, 126, 129, 224, 413, 801
SspMI CTAG 4 cut(s) 78, 183, 479, 1194
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 1 cut(s) 1303
TaaI ACNGT 7 cut(s) 112, 210, 215, 1064, 1078, 1156, 1210
TaiI ACGT 4 cut(s) 42, 234, 693, 739
TaqI TCGA 2 cut(s) 277, 319
TasI AATT 7 cut(s) 562, 593, 722, 942, 1002, 1027, 1142
TatI WGTACW 1 cut(s) 210
TauI GCSGC 2 cut(s) 131, 804
TfiI GAWTC 2 cut(s) 475, 659
Tru1I TTAA 1 cut(s) 1059
Tru9I TTAA 1 cut(s) 1059
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 1 cut(s) 614
TseI GCWGC 2 cut(s) 120, 1334
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 4 cut(s) 420, 1011, 1067, 1329
TspGWI ACGGA 4 cut(s) 34, 180, 517, 984
TspRI CASTG 1 cut(s) 523
VpaK11BI GGWCC 1 cut(s) 379
XapI RAATTY 2 cut(s) 593, 1142
XhoI CTCGAG 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 450
XspI CTAG 4 cut(s) 78, 183, 479, 1194
ZraI GACGTC 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.