pycom06g19420

Part of the AP-3 complex, an adaptor-related complex which seems to be clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. It also function in maintaining the identity of lytic vacuoles and in regulating the transition between storage and lytic vacuoles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0011187
Physical Location & Seq
Forward (+)
22852025 .. 22854664
2640 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19420.1

Sequence Viewer

Length: 2640 bp
ATGGCGGGTTCCTCCCTCATGGAAAACCTCTTCCAGCGCACGCTGGAGGACCTAATCAAAGGCCTCCGCCTCCAGCTCATCGGCGAGTCCGCCTTCCTCTCCAAAGCCCTCGACGAAATCCGCCGCGAGATCAAATCCACCGACGCCGACACTAAAGCCAACGCCCTCCACAAGCTCACCTACCTCTCCTCCCTCCACTTCTTCGACATGTCCTTCGCTGCCTTCCACGTCGTCGAGCTCCTCTCCTCCACCCGCTTTTCCCACAAGAAGATCGCCTACCACGCCGCCGCCCACTCCTTCTCCGACAACACCCCGGTCCTCGTCCTCATCACCAACCAGCTCCGCAAGGACCTCACCTCTACCAACGAGCTCGAGGTAAGCTTAGCGCTCGAATGCCTTTCTAGAATTGCTACTGTAGATCTTGCTAGGGATTTGACACCAGAGGTTTTCACCTTGCTGGGTAGCGGCAAGGTTTTCGTCCGAAAGAAAGCAACCGGCGTGCTTTTGAGGGTGTTTGATAAATACCCAGATGCTGTGAGGGTGTGTTTTAAGCGTTTGGTTGAGAATTTGGAGAGTCCTGACTCGCAGATTGTGTCTGTGGCTGTTGGGGTGTTTTGTGAGCTTGCTTTGAGAGAACCCAGGTCATATCTTCCGTTGGCGCCTGAGTTTTATAAGATCTTGGTTGATTCCAAGAACAACTGGGTTTTGATTAAGGTGTTGAAGATATTTGCAAAGTTGGCTCCTTTAGAACCTAGGTTGGCGAAGAGGGTTGTTGAGCCGGTCTGTGAACATATCAGGAGGACTGGGGCCAAATCATTGTTGTTTGAGTGTATTAGGACAGTGGTGACCGGTTTGAGTGATTATGAATCTGCAGTGAAGGTTGTCGTTGTGAAGATTCGGGAAATGCTAGTTGATGATGATCCGAATCTCAAGTATCTTGCATTGCAGGCACTTTCGGTGGTTGCCCCAAAGCACTTGTGGGCAGTGTTGGAGAATAAGGAGGTTGTGATTAAGTCTTTGAGTGATGTGGATACAAATATCAAGCTGGAGTCTTTGCGTCTTGTTATGGCAATGCTGTCTGAGAATAATGTGGCTGAAATTTGCAGGGTGTTGGTCAATTATGCTCTTAAATCTGATCCCGAGTTTTGCAATGAGATTTTGGATTCCATTTTATCAACATGCTGCAGTAATGTGTACGAGATTATTGTTGACTTCGATTGGTATGTATCACTTCTGGGAGAAATGGCGAGGATCCCGCATTGCCAGAAGGGGGAAGAAATTGAGAAGCAGCTTATTGATATTGGTATGAGGGTCAAGGATGTTAGACCAGAGCTTGTCCGGGTCAGTCGTGATCTGCTGATTGATCCTGCATTACTCGGTAATCCTTTCTTACACAGGATATTATCAGCCGCTGCTTGGTTGTCAGGGCAACATGTTGAATTCTCGATAAACCCGTTTGAGCTCATGGAGGCACTATTACAGCCTCGTACAATTCTCTTGGCACCATTTATAAGAGCGATATATGTACAGTCTGGTCTTAAAGTTTTAGTCTTTTGTTTAAATTCTTACCTTTTGCAGAGGTGGAATACTGCTTCCTCCTCAACTTTTGATGGATTGGTTTTGGAACGAGTTGACCCAGAGAGTTCTATTTTGGCATCATGTGATGCTCCTGTGCATTGCATACAGGATGAAGGATTCAACCCGAGGGTTTTAAATCAATCTTTTGAAGGTCTTTCTGTGGAATATGATGGGGAGGAAACTGCTACTCTTGGGCAGACATCTGCATCGTCTTCATTGAAGGACAGTTTCACATGTGAATCTATCATAAACCTTTTAAATCGAGTTGAACTGGCTGTGACTCCATTAATGGGAAGCTATGATGTAGAAATACTAGAGAGAGCACGGAATATACTTTGTTACATCCTGTTGATTAGGCAAGAACTGCTTGATTGTCCAGTACACAGGGAAGAAAGTTTGGAAAGTGAAGAAGCACAAGCGTCCCAAATCATCAGAGTGATGCATGATGCCTTTTCAAGTGATCTAGGTCCTGTCTCAGTGAGTGCTCAAGAAAGAGTTCCTGTACCAGATGGGTTAATGCTTGCAGAGAATCTTGAAGACTTGGAAAAAATCTTCGGTGATGTTCAACTACCTTCGTTAAGTTCATTTTCTCTTGGAAGCCCACAGTATGAGGAGAGAGCTGGTTTTTCTATTCCTATCCATGAAAGCAAAGAAGAGCCAGTGCCATCGAATGAATCCACGTCTCTGCTTGCAGAGCACCGTAAGCAGCACGGTTTATATTATCTTCCCTCTGTGAAGGAGGAAGATAATTATCCACCTGCCAATGATCCTAAATTACAGGTTGATACTAATGAGGATGATGAAGATCTAGTTAAATTACTTGTTTCAAAGAAAAAGCCATGTCATGCAAAGCCTCGGCCTGTGGTGGTGAAATTAGATGGAGATCAAGCACCTATTGCAGCCAACCCTCGCCCGAAGGAAGATGTGTTGTCTGGTACCATGCGAGACGTTCTTTCAGGTAGCGACACCAATCCCACCTCATCTCAGAGTAGAGTATCCACCAAGTCATCGAGTAAGAGAAAAGGGAAGGAGAAACTAAATGTTGATCGATCACGCTCCTAA

Protein Analysis

880

Amino Acids

98.13

Weight (kDa)

5.39

Isoelectric Point (pI)

44.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 34 - 491 1.1e-80 Adaptin N terminal region
Cnd1 PF12717 128 - 270 2.9e-08 non-SMC mitotic condensation complex subunit 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48760 AT1G48760 AT1G48760
fragaria_vesca FvH4_5g11850
malus_domestica MD06G1218100.v1.1 MD14G1228200.v1.1
prunus_persica Prupe.5G223200_v2.0.a1
pyrus_communis pycom06g19420 pycom14g18960
rosa_chinensis RchiOBHm_Chr7g0185741
rosa_laevigata RLG00000004886
rosa_multiflora Rmu_sc0002121.1_g000007 Rmu_sc0002952.1_g000005
rosa_roxburghii Rroxscaffold_3G00268650
rosa_rugosa Rorug06G0471100
rosa_samantha Rh7AG071300 Rh7BG076600 Rh7CG076600 Rh7DG076400
rosa_wichuraiana Rw7G006380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 672, 1511
AarI CACCTGC 1 cut(s) 2344
Acc36I ACCTGC 1 cut(s) 2344
Acc65I GGTACC 1 cut(s) 2513
AccB1I GGYRCC 3 cut(s) 658, 1501, 2513
AccII CGCG 1 cut(s) 126
AclWI GGATC 6 cut(s) 914, 1130, 1246, 1259, 1358, 2339
AcsI RAATTY 4 cut(s) 565, 1098, 1439, 1561
AcyI GRCGYC 2 cut(s) 144, 659
AfaI GTAC 6 cut(s) 1196, 1489, 1527, 1959, 2082, 2515
AfeI AGCGCT 1 cut(s) 387
AfiI CCNNNNNNNGG 3 cut(s) 1270, 1416, 1868
AflIII ACRYGT 3 cut(s) 207, 1432, 1811
AgeI ACCGGT 1 cut(s) 848
AjiI CACGTC 2 cut(s) 229, 2259
AjnI CCWGG 1 cut(s) 638
Alw21I GWGCWC 6 cut(s) 240, 372, 1464, 1903, 2065, 2277
Alw26I GTCTC 3 cut(s) 2056, 2265, 2517
AlwI GGATC 6 cut(s) 914, 1130, 1246, 1259, 1358, 2339
AlwNI CAGNNNCTG 2 cut(s) 533, 1412
Ama87I CYCGRG 3 cut(s) 371, 1139, 1702
Aor51HI AGCGCT 1 cut(s) 387
AoxI GGCC 3 cut(s) 61, 807, 2435
ApeKI GCWGC 6 cut(s) 218, 1182, 1288, 1412, 2284, 2477
ApoI RAATTY 4 cut(s) 565, 1098, 1439, 1561
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AseI ATTAAT 1 cut(s) 1865
AsiGI ACCGGT 1 cut(s) 848
Asp700I GAANNNNTTC 1 cut(s) 2073
Asp718I GGTACC 1 cut(s) 2513
AspA2I CCTAGG 1 cut(s) 752
AspLEI GCGC 3 cut(s) 39, 388, 661
AspS9I GGNCC 5 cut(s) 49, 316, 349, 807, 2045
AsuC2I CCSGG 2 cut(s) 314, 1340
AsuHPI GGTGA 7 cut(s) 169, 322, 346, 442, 856, 2147, 2458
AvaI CYCGRG 3 cut(s) 371, 1139, 1702
AvaII GGWCC 4 cut(s) 49, 316, 349, 2045
AvrII CCTAGG 1 cut(s) 752
BamHI GGATCC 1 cut(s) 1251
BanI GGYRCC 3 cut(s) 658, 1501, 2513
BanII GRGCYC 3 cut(s) 240, 372, 1464
BarI GAAGNNNNNNTAC 2 cut(s) 260, 292
BbsI GAAGAC 2 cut(s) 1782, 2121
Bbv12I GWGCWC 6 cut(s) 240, 372, 1464, 1903, 2065, 2277
BbvI GCAGC 6 cut(s) 205, 1169, 1300, 1399, 2296, 2489
BccI CCATC 5 cut(s) 1604, 1742, 2081, 2251, 2450
BcgI CGANNNNNNTGC 2 cut(s) 457, 491
BciT130I CCWGG 1 cut(s) 640
BciVI GTATCC 2 cut(s) 1024, 2584
BcnI CCSGG 2 cut(s) 314, 1340
BcoDI GTCTC 3 cut(s) 2056, 2265, 2517
BfaI CTAG 7 cut(s) 402, 426, 753, 908, 1892, 2042, 2387
BfmI CTRYAG 3 cut(s) 414, 870, 1183
BfoI RGCGCY 2 cut(s) 389, 662
BfuAI ACCTGC 1 cut(s) 2344
BfuI GTATCC 2 cut(s) 1024, 2584
BglII AGATCT 3 cut(s) 418, 675, 2383
BlnI CCTAGG 1 cut(s) 752
BlpI GCTNAGC 1 cut(s) 382
Bme1390I CCNGG 3 cut(s) 314, 640, 1340
Bme18I GGWCC 4 cut(s) 49, 316, 349, 2045
BmeT110I CYCGRG 3 cut(s) 371, 1139, 1702
BmgBI CACGTC 2 cut(s) 229, 2259
BmgT120I GGNCC 5 cut(s) 49, 316, 349, 807, 2045
BmiI GGNNCC 7 cut(s) 10, 660, 741, 808, 1253, 1503, 2515
BmrFI CCNGG 3 cut(s) 314, 640, 1340
BmrI ACTGGG 2 cut(s) 709, 813
BmsI GCATC 6 cut(s) 520, 1654, 1664, 1793, 2007, 2014
BmuI ACTGGG 2 cut(s) 709, 813
BpiI GAAGAC 2 cut(s) 1782, 2121
BplI GAGNNNNNCTC 2 cut(s) 227, 259
BpmI CTGGAG 3 cut(s) 56, 65, 1067
Bpu1102I GCTNAGC 1 cut(s) 382
BpuEI CTTGAG 2 cut(s) 914, 2049
BpuMI CCSGG 2 cut(s) 314, 1340
Bsa29I ATCGAT 1 cut(s) 2626
BsaBI GATNNNNATC 5 cut(s) 918, 924, 2328, 2382, 2460
BsaHI GRCGYC 2 cut(s) 144, 659
BsaJI CCNNGG 5 cut(s) 312, 638, 752, 1703, 2432
BsaWI WCCGGW 1 cut(s) 848
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 284, 314
Bsc4I CCNNNNNNNGG 3 cut(s) 1270, 1416, 1868
Bse118I RCCGGY 3 cut(s) 494, 778, 848
Bse1I ACTGG 5 cut(s) 704, 808, 1854, 1955, 2237
Bse3DI GCAATG 5 cut(s) 941, 1077, 1156, 1258, 1675
Bse8I GATNNNNATC 5 cut(s) 918, 924, 2328, 2382, 2460
BseBI CCWGG 1 cut(s) 640
BseCI ATCGAT 1 cut(s) 2626
BseDI CCNNGG 5 cut(s) 312, 638, 752, 1703, 2432
BseGI GGATG 4 cut(s) 1324, 1693, 1920, 2380
BseJI GATNNNNATC 5 cut(s) 918, 924, 2328, 2382, 2460
BseLI CCNNNNNNNGG 3 cut(s) 1270, 1416, 1868
BseMI GCAATG 5 cut(s) 941, 1077, 1156, 1258, 1675
BseMII CTCAG 4 cut(s) 654, 1071, 2067, 2576
BseNI ACTGG 5 cut(s) 704, 808, 1854, 1955, 2237
BseRI GAGGAG 5 cut(s) 178, 230, 235, 1588, 2204
BseXI GCAGC 6 cut(s) 205, 1169, 1300, 1399, 2296, 2489
BseYI CCCAGC 1 cut(s) 457
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 3 cut(s) 63, 809, 2437
BshNI GGYRCC 3 cut(s) 658, 1501, 2513
BshTI ACCGGT 1 cut(s) 848
BshVI ATCGAT 1 cut(s) 2626
BsiHKAI GWGCWC 6 cut(s) 240, 372, 1464, 1903, 2065, 2277
BsiHKCI CYCGRG 3 cut(s) 371, 1139, 1702
BsiSI CCGG 5 cut(s) 314, 495, 779, 849, 1339
BslFI GGGAC 1 cut(s) 1984
BslI CCNNNNNNNGG 3 cut(s) 1270, 1416, 1868
BsmAI GTCTC 3 cut(s) 2056, 2265, 2517
BsmBI CGTCTC 2 cut(s) 2265, 2517
BsmFI GGGAC 1 cut(s) 1984
BsmI GAATGC 1 cut(s) 398
BsnI GGCC 3 cut(s) 63, 809, 2437
BsoBI CYCGRG 3 cut(s) 371, 1139, 1702
Bsp1286I GDGCHC 6 cut(s) 240, 372, 1464, 1903, 2065, 2277
Bsp1407I TGTACA 1 cut(s) 1525
Bsp1720I GCTNAGC 1 cut(s) 382
BspANI GGCC 3 cut(s) 63, 809, 2437
BspCNI CTCAG 4 cut(s) 655, 1072, 2066, 2575
BspDI ATCGAT 1 cut(s) 2626
BspFNI CGCG 1 cut(s) 126
BspLI GGNNCC 7 cut(s) 10, 660, 741, 808, 1253, 1503, 2515
BspMAI CTGCAG 2 cut(s) 874, 1187
BspMI ACCTGC 1 cut(s) 2344
BspPI GGATC 6 cut(s) 914, 1130, 1246, 1259, 1358, 2339
BspQI GCTCTTC 1 cut(s) 2226
BspT107I GGYRCC 3 cut(s) 658, 1501, 2513
BsrDI GCAATG 5 cut(s) 941, 1077, 1156, 1258, 1675
BsrFI RCCGGY 3 cut(s) 494, 778, 848
BsrGI TGTACA 1 cut(s) 1525
BsrI ACTGG 5 cut(s) 704, 808, 1854, 1955, 2237
BssAI RCCGGY 3 cut(s) 494, 778, 848
BssECI CCNNGG 5 cut(s) 312, 638, 752, 1703, 2432
BssNI GRCGYC 2 cut(s) 144, 659
BssT1I CCWWGG 1 cut(s) 752
Bst2UI CCWGG 1 cut(s) 640
Bst4CI ACNGT 7 cut(s) 415, 841, 1530, 1805, 2184, 2279, 2291
Bst6I CTCTTC 3 cut(s) 35, 758, 2226
BstACI GRCGYC 2 cut(s) 144, 659
BstAPI GCANNNNNTGC 2 cut(s) 1942, 2474
BstAUI TGTACA 1 cut(s) 1525
BstC8I GCNNGC 6 cut(s) 41, 500, 624, 948, 2100, 2268
BstDEI CTNAG 5 cut(s) 382, 663, 1080, 2053, 2562
BstEII GGTNACC 1 cut(s) 844
BstF5I GGATG 4 cut(s) 1324, 1693, 1920, 2380
BstFNI CGCG 1 cut(s) 126
BstH2I RGCGCY 2 cut(s) 389, 662
BstHHI GCGC 3 cut(s) 39, 388, 661
BstMAI GTCTC 3 cut(s) 2056, 2265, 2517
BstMWI GCNNNNNNNGC 7 cut(s) 281, 737, 947, 1942, 2272, 2281, 2474
BstNI CCWGG 1 cut(s) 640
BstNSI RCATGY 4 cut(s) 211, 1182, 1436, 1815
BstPI GGTNACC 1 cut(s) 844
BstSCI CCNGG 3 cut(s) 312, 638, 1338
BstSFI CTRYAG 3 cut(s) 414, 870, 1183
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 6 cut(s) 205, 1169, 1300, 1399, 2296, 2489
BstV2I GAAGAC 2 cut(s) 1782, 2121
BstX2I RGATCY 4 cut(s) 418, 675, 1251, 2383
BstYI RGATCY 4 cut(s) 418, 675, 1251, 2383
Bsu15I ATCGAT 1 cut(s) 2626
BsuI GTATCC 2 cut(s) 1024, 2584
BsuRI GGCC 3 cut(s) 63, 809, 2437
BsuTUI ATCGAT 1 cut(s) 2626
BtrI CACGTC 2 cut(s) 229, 2259
BtsCI GGATG 4 cut(s) 1324, 1693, 1920, 2380
BtsI GCAGTG 2 cut(s) 879, 990
BtsIMutI CAGTG 5 cut(s) 846, 879, 990, 2061, 2244
BveI ACCTGC 1 cut(s) 2344
Cac8I GCNNGC 6 cut(s) 41, 500, 624, 948, 2100, 2268
CaiI CAGNNNCTG 2 cut(s) 533, 1412
CfoI GCGC 3 cut(s) 39, 388, 661
Cfr10I RCCGGY 3 cut(s) 494, 778, 848
Cfr13I GGNCC 5 cut(s) 49, 316, 349, 807, 2045
ClaI ATCGAT 1 cut(s) 2626
CseI GACGC 3 cut(s) 152, 1046, 1986
Csp6I GTAC 6 cut(s) 1195, 1488, 1526, 1958, 2081, 2514
CspAI ACCGGT 1 cut(s) 848
CviQI GTAC 6 cut(s) 1195, 1488, 1526, 1958, 2081, 2514
DdeI CTNAG 5 cut(s) 382, 663, 1080, 2053, 2562
DinI GGCGCC 1 cut(s) 660
DraI TTTAAA 3 cut(s) 1560, 1713, 1836
Eam1104I CTCTTC 3 cut(s) 35, 758, 2226
EarI CTCTTC 3 cut(s) 35, 758, 2226
EciI GGCGGA 3 cut(s) 56, 79, 110
Ecl136II GAGCTC 3 cut(s) 238, 370, 1462
Eco130I CCWWGG 1 cut(s) 752
Eco147I AGGCCT 1 cut(s) 63
Eco24I GRGCYC 3 cut(s) 240, 372, 1464
Eco47I GGWCC 4 cut(s) 49, 316, 349, 2045
Eco47III AGCGCT 1 cut(s) 387
Eco53kI GAGCTC 3 cut(s) 238, 370, 1462
Eco88I CYCGRG 3 cut(s) 371, 1139, 1702
Eco91I GGTNACC 1 cut(s) 844
EcoICRI GAGCTC 3 cut(s) 238, 370, 1462
EcoO109I RGGNCCY 3 cut(s) 49, 349, 2045
EcoO65I GGTNACC 1 cut(s) 844
EcoRI GAATTC 1 cut(s) 1439
EcoRII CCWGG 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 752
EcoT22I ATGCAT 1 cut(s) 2022
EcoT38I GRGCYC 3 cut(s) 240, 372, 1464
EgeI GGCGCC 1 cut(s) 660
EheI GGCGCC 1 cut(s) 660
ErhI CCWWGG 1 cut(s) 752
Esp3I CGTCTC 2 cut(s) 2265, 2517
FalI AAGNNNNNCTT 2 cut(s) 1929, 1961
FaqI GGGAC 1 cut(s) 1984
FauI CCCGC 2 cut(s) 260, 1263
FokI GGATG 4 cut(s) 1331, 1700, 1907, 2387
FriOI GRGCYC 3 cut(s) 240, 372, 1464
FspBI CTAG 7 cut(s) 402, 426, 753, 908, 1892, 2042, 2387
GlaI GCGC 3 cut(s) 38, 387, 660
GsaI CCCAGC 1 cut(s) 461
GsuI CTGGAG 3 cut(s) 56, 65, 1067
HaeII RGCGCY 2 cut(s) 389, 662
HaeIII GGCC 3 cut(s) 63, 809, 2437
HapII CCGG 5 cut(s) 314, 495, 779, 849, 1339
HgaI GACGC 3 cut(s) 152, 1046, 1986
HhaI GCGC 3 cut(s) 39, 388, 661
Hin1I GRCGYC 2 cut(s) 144, 659
Hin6I GCGC 3 cut(s) 37, 386, 659
HinP1I GCGC 3 cut(s) 37, 386, 659
HincII GTYRAC 2 cut(s) 1210, 1633
HindII GTYRAC 2 cut(s) 1210, 1633
HindIII AAGCTT 1 cut(s) 379
HpaII CCGG 5 cut(s) 314, 495, 779, 849, 1339
HphI GGTGA 7 cut(s) 169, 322, 346, 442, 856, 2147, 2458
Hpy166II GTNNAC 5 cut(s) 788, 1195, 1210, 1633, 1960
Hpy188I TCNGA 7 cut(s) 304, 482, 924, 1081, 1135, 2012, 2565
Hpy188III TCNNGA 9 cut(s) 402, 578, 796, 899, 1139, 1349, 1444, 2066, 2111
Hpy8I GTNNAC 5 cut(s) 788, 1195, 1210, 1633, 1960
Hpy99I CGWCG 4 cut(s) 116, 146, 233, 236
HpyCH4III ACNGT 7 cut(s) 415, 841, 1530, 1805, 2184, 2279, 2291
HpyCH4IV ACGT 3 cut(s) 228, 2258, 2526
HpyF10VI GCNNNNNNNGC 7 cut(s) 281, 737, 947, 1942, 2272, 2281, 2474
HpyF3I CTNAG 5 cut(s) 382, 663, 1080, 2053, 2562
HpySE526I ACGT 3 cut(s) 228, 2258, 2526
Hsp92I GRCGYC 2 cut(s) 144, 659
HspAI GCGC 3 cut(s) 37, 386, 659
KasI GGCGCC 1 cut(s) 658
KpnI GGTACC 1 cut(s) 2517
LguI GCTCTTC 1 cut(s) 2226
LmnI GCTCC 5 cut(s) 243, 345, 745, 1672, 2639
Lsp1109I GCAGC 6 cut(s) 205, 1169, 1300, 1399, 2296, 2489
LweI GCATC 6 cut(s) 520, 1654, 1664, 1793, 2007, 2014
MaeI CTAG 7 cut(s) 402, 426, 753, 908, 1892, 2042, 2387
MaeII ACGT 3 cut(s) 228, 2258, 2526
MaeIII GTNAC 3 cut(s) 844, 1855, 1916
MflI RGATCY 4 cut(s) 418, 675, 1251, 2383
MhlI GDGCHC 6 cut(s) 240, 372, 1464, 1903, 2065, 2277
Mly113I GGCGCC 1 cut(s) 659
MlyI GAGTC 5 cut(s) 95, 575, 583, 1058, 1852
MmeI TCCRAC 2 cut(s) 327, 969
Mph1103I ATGCAT 1 cut(s) 2022
MroXI GAANNNNTTC 1 cut(s) 2073
MslI CAYNNNNRTG 1 cut(s) 2012
MspA1I CMGCKG 1 cut(s) 1412
MspI CCGG 5 cut(s) 314, 495, 779, 849, 1339
MspR9I CCNGG 3 cut(s) 314, 640, 1340
Mva1269I GAATGC 1 cut(s) 398
MvaI CCWGG 1 cut(s) 640
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 7 cut(s) 281, 737, 947, 1942, 2272, 2281, 2474
NarI GGCGCC 1 cut(s) 659
NciI CCSGG 2 cut(s) 314, 1340
NlaIV GGNNCC 7 cut(s) 10, 660, 741, 808, 1253, 1503, 2515
NmeAIII GCCGAG 1 cut(s) 2413
NmuCI GTSAC 2 cut(s) 844, 1855
NsiI ATGCAT 1 cut(s) 2022
NspI RCATGY 4 cut(s) 211, 1182, 1436, 1815
PaeR7I CTCGAG 1 cut(s) 371
PaqCI CACCTGC 1 cut(s) 2344
PceI AGGCCT 1 cut(s) 63
PciI ACATGT 3 cut(s) 207, 1432, 1811
PciSI GCTCTTC 1 cut(s) 2226
PcsI WCGNNNNNNNCGW 1 cut(s) 1451
PctI GAATGC 1 cut(s) 398
PdmI GAANNNNTTC 1 cut(s) 2073
PfeI GAWTC 9 cut(s) 686, 866, 895, 925, 1163, 1695, 1817, 2107, 2252
PinAI ACCGGT 1 cut(s) 848
PleI GAGTC 5 cut(s) 94, 575, 582, 1057, 1852
PluTI GGCGCC 1 cut(s) 662
PpsI GAGTC 5 cut(s) 94, 575, 582, 1057, 1852
PpuMI RGGWCCY 3 cut(s) 49, 349, 2045
PscI ACATGT 3 cut(s) 207, 1432, 1811
PshBI ATTAAT 1 cut(s) 1865
PsiI TTATAA 2 cut(s) 672, 1511
Psp124BI GAGCTC 3 cut(s) 240, 372, 1464
Psp5II RGGWCCY 3 cut(s) 49, 349, 2045
Psp6I CCWGG 1 cut(s) 638
PspEI GGTNACC 1 cut(s) 844
PspFI CCCAGC 1 cut(s) 457
PspGI CCWGG 1 cut(s) 638
PspN4I GGNNCC 7 cut(s) 10, 660, 741, 808, 1253, 1503, 2515
PspPI GGNCC 5 cut(s) 49, 316, 349, 807, 2045
PspPPI RGGWCCY 3 cut(s) 49, 349, 2045
PspXI VCTCGAGB 1 cut(s) 371
PstI CTGCAG 2 cut(s) 874, 1187
PstNI CAGNNNCTG 2 cut(s) 533, 1412
PsuI RGATCY 4 cut(s) 418, 675, 1251, 2383
RsaI GTAC 6 cut(s) 1196, 1489, 1527, 1959, 2082, 2515
RsaNI GTAC 6 cut(s) 1195, 1488, 1526, 1958, 2081, 2514
RseI CAYNNNNRTG 1 cut(s) 2012
SacI GAGCTC 3 cut(s) 240, 372, 1464
SapI GCTCTTC 1 cut(s) 2226
Sau96I GGNCC 5 cut(s) 49, 316, 349, 807, 2045
SchI GAGTC 5 cut(s) 95, 575, 583, 1058, 1852
ScrFI CCNGG 3 cut(s) 314, 640, 1340
SduI GDGCHC 6 cut(s) 240, 372, 1464, 1903, 2065, 2277
SfaNI GCATC 6 cut(s) 520, 1654, 1664, 1793, 2007, 2014
SfcI CTRYAG 3 cut(s) 414, 870, 1183
SfoI GGCGCC 1 cut(s) 660
Sfr274I CTCGAG 1 cut(s) 371
SinI GGWCC 4 cut(s) 49, 316, 349, 2045
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 1 cut(s) 2012
SmlI CTYRAG 3 cut(s) 371, 929, 2064
SmoI CTYRAG 3 cut(s) 371, 929, 2064
SseBI AGGCCT 1 cut(s) 63
SspDI GGCGCC 1 cut(s) 658
SspMI CTAG 7 cut(s) 402, 426, 753, 908, 1892, 2042, 2387
SstI GAGCTC 3 cut(s) 240, 372, 1464
StuI AGGCCT 1 cut(s) 63
StyD4I CCNGG 3 cut(s) 312, 638, 1338
StyI CCWWGG 1 cut(s) 752
TaaI ACNGT 7 cut(s) 415, 841, 1530, 1805, 2184, 2279, 2291
TaiI ACGT 3 cut(s) 231, 2261, 2529
TatI WGTACW 2 cut(s) 1525, 1957
TauI GCSGC 5 cut(s) 126, 287, 290, 468, 1412
TfiI GAWTC 9 cut(s) 686, 866, 895, 925, 1163, 1695, 1817, 2107, 2252
TscAI CASTG 5 cut(s) 846, 879, 990, 2061, 2244
TseFI GTSAC 2 cut(s) 844, 1855
TseI GCWGC 6 cut(s) 218, 1182, 1288, 1412, 2284, 2477
Tsp45I GTSAC 2 cut(s) 844, 1855
TspDTI ATGAA 7 cut(s) 879, 1704, 1782, 2151, 2235, 2265, 2394
TspGWI ACGGA 2 cut(s) 642, 1918
TspRI CASTG 5 cut(s) 846, 879, 990, 2061, 2244
VpaK11BI GGWCC 4 cut(s) 49, 316, 349, 2045
VspI ATTAAT 1 cut(s) 1865
XapI RAATTY 4 cut(s) 565, 1098, 1439, 1561
XbaI TCTAGA 1 cut(s) 401
XceI RCATGY 4 cut(s) 211, 1182, 1436, 1815
XcmI CCANNNNNNNNNTGG 1 cut(s) 975
XhoI CTCGAG 1 cut(s) 371
XmaJI CCTAGG 1 cut(s) 752
XmnI GAANNNNTTC 1 cut(s) 2073
XspI CTAG 7 cut(s) 402, 426, 753, 908, 1892, 2042, 2387
Zsp2I ATGCAT 1 cut(s) 2022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.