Rorug06G0471100

Part of the AP-3 complex, an adaptor-related complex which seems to be clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to the vacuole. It also function in maintaining the identity of lytic vacuoles and in regulating the transition between storage and lytic vacuoles

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0011187
Physical Location & Seq
Reverse (-)
59414057 .. 59415518
1462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0471100.1

Sequence Viewer

Length: 669 bp
ATGGGGTATGGTTCGCCAACGTGGTTCAGAATTGGCGACGCATTAACATATCGCGGAGCTGCATATCATGGCTTGGTTGAGGAAGATACTTCGCTGACCATACCTAGTCAGCTTTGGCTGACCATAACCACTAAGTGGGAGACACGTGTCCCCCCAAGCCAGTTAAGCCTTCTACACGCGCTCGATCCCGTCGGTGGTGCTGTGCTTCATGACGGGGATGGAGGTGGAGGAAGCAAGCATAGTACTGCTGCGAAGCAGGGTGGTACCCCGGAGGAGGATTTGCCGTTGCCGGAGACGTGCCGTTACCGGAGACAAGTAATGATTTTTTCTGACAATGCTACTAAGGGATTCAGCATAATTTGGCTCATCTCTAAAATAGTGGAAACGGGCATGACCTACTGGCCTGAGCAGACGGCCATTAAGAAGGTCCCAATTCACTGGAGCAAGGAGCAAGTCCGCGATCTTTTCAAAAGCTTTGATAGGAACGGTGACGGAAAACTCTCCAAGGAAGAGCTCAAGGCAGCATTTCGGAAACTCGGATCGCGTTGGAGCTCTTACAGAGCTAGGAGAGCATTACGCCATGCAGATTCCAATGGCGACAGCATCATCTCTAATGAGGAGCTCGACGATCTTATAAACTATGCCCTAAAATGTGGTTATAAGCTATAA

Protein Analysis

222

Amino Acids

24.83

Weight (kDa)

8.44

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 143 - 177 4.8e-06 EF-hand domain pair
EF-hand_7 PF13499 150 - 179 2e-06 EF-hand domain pair
EF-hand_6 PF13405 152 - 180 7.2e-09 EF-hand domain
EF-hand_1 PF00036 152 - 179 8.9e-08 EF hand domain
EF-hand_5 PF13202 153 - 174 7.7e-08 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011187)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48760 AT1G48760 AT1G48760
fragaria_vesca FvH4_5g11850
malus_domestica MD06G1218100.v1.1 MD14G1228200.v1.1
prunus_persica Prupe.5G223200_v2.0.a1
pyrus_communis pycom06g19420 pycom14g18960
rosa_chinensis RchiOBHm_Chr7g0185741
rosa_laevigata RLG00000004886
rosa_multiflora Rmu_sc0002121.1_g000007 Rmu_sc0002952.1_g000005
rosa_roxburghii Rroxscaffold_3G00268650
rosa_rugosa Rorug06G0471100
rosa_samantha Rh7AG071300 Rh7BG076600 Rh7CG076600 Rh7DG076400
rosa_wichuraiana Rw7G006380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 635, 660
Acc65I GGTACC 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 263
AccB7I CCANNNNNTGG 1 cut(s) 135
AccII CGCG 4 cut(s) 54, 179, 459, 544
AciI CCGC 2 cut(s) 54, 457
AclWI GGATC 2 cut(s) 179, 547
AcoI YGGCCR 1 cut(s) 414
AcvI CACGTG 1 cut(s) 146
AdeI CACNNNGTG 1 cut(s) 135
AfaI GTAC 2 cut(s) 244, 265
AfiI CCNNNNNNNGG 3 cut(s) 135, 194, 274
AflIII ACRYGT 2 cut(s) 143, 145
AgsI TTSAA 1 cut(s) 469
AjiI CACGTC 1 cut(s) 297
AluBI AGCT 8 cut(s) 59, 112, 474, 514, 552, 563, 622, 664
AluI AGCT 8 cut(s) 59, 112, 474, 514, 552, 563, 622, 664
Alw21I GWGCWC 3 cut(s) 516, 554, 624
Alw26I GTCTC 3 cut(s) 134, 287, 304
AlwI GGATC 2 cut(s) 179, 547
AoxI GGCC 2 cut(s) 401, 414
ApeKI GCWGC 3 cut(s) 59, 248, 521
Asp718I GGTACC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 1 cut(s) 427
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 500
AvaII GGWCC 1 cut(s) 427
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 3 cut(s) 516, 554, 624
BbrPI CACGTG 1 cut(s) 146
Bbv12I GWGCWC 3 cut(s) 516, 554, 624
BbvI GCAGC 3 cut(s) 46, 235, 533
BccI CCATC 1 cut(s) 212
BceAI ACGGC 3 cut(s) 268, 285, 429
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 3 cut(s) 134, 287, 304
BfaI CTAG 2 cut(s) 105, 564
BisI GCNGC 3 cut(s) 60, 249, 522
BlsI GCNGC 3 cut(s) 61, 250, 523
BmcAI AGTACT 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 269
Bme18I GGWCC 1 cut(s) 427
BmgBI CACGTC 1 cut(s) 297
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 2 cut(s) 265, 429
BmrFI CCNGG 1 cut(s) 269
BmsI GCATC 1 cut(s) 612
BoxI GACNNNNGTC 1 cut(s) 146
BpmI CTGGAG 1 cut(s) 460
Bpu10I CCTNAGC 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 500
BpuMI CCSGG 1 cut(s) 269
BsaAI YACGTR 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 267, 504
BsaWI WCCGGW 1 cut(s) 306
Bsc4I CCNNNNNNNGG 3 cut(s) 135, 194, 274
Bse1I ACTGG 3 cut(s) 160, 404, 443
BseDI CCNNGG 2 cut(s) 267, 504
BseGI GGATG 1 cut(s) 223
BseLI CCNNNNNNNGG 3 cut(s) 135, 194, 274
BseMII CTCAG 1 cut(s) 396
BseNI ACTGG 3 cut(s) 160, 404, 443
BseRI GAGGAG 2 cut(s) 287, 632
BseXI GCAGC 3 cut(s) 46, 235, 533
Bsh1236I CGCG 4 cut(s) 54, 179, 459, 544
BshFI GGCC 2 cut(s) 403, 416
BshNI GGYRCC 1 cut(s) 263
BsiHKAI GWGCWC 3 cut(s) 516, 554, 624
BsiSI CCGG 3 cut(s) 269, 290, 307
BslFI GGGAC 2 cut(s) 134, 413
BslI CCNNNNNNNGG 3 cut(s) 135, 194, 274
BsmAI GTCTC 3 cut(s) 134, 287, 304
BsmBI CGTCTC 1 cut(s) 287
BsmFI GGGAC 2 cut(s) 134, 413
BsnI GGCC 2 cut(s) 403, 416
Bsp1286I GDGCHC 3 cut(s) 516, 554, 624
Bsp143I GATC 4 cut(s) 184, 460, 539, 628
BspACI CCGC 2 cut(s) 54, 457
BspANI GGCC 2 cut(s) 403, 416
BspCNI CTCAG 1 cut(s) 397
BspFNI CGCG 4 cut(s) 54, 179, 459, 544
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 265, 429
BspPI GGATC 2 cut(s) 179, 547
BspQI GCTCTTC 1 cut(s) 504
BspT107I GGYRCC 1 cut(s) 263
BsrI ACTGG 3 cut(s) 160, 404, 443
BssECI CCNNGG 2 cut(s) 267, 504
BssMI GATC 4 cut(s) 184, 460, 539, 628
BssT1I CCWWGG 1 cut(s) 504
Bst4CI ACNGT 1 cut(s) 488
Bst6I CTCTTC 1 cut(s) 504
BstBAI YACGTR 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 236
BstDEI CTNAG 3 cut(s) 132, 342, 405
BstF5I GGATG 1 cut(s) 223
BstFNI CGCG 4 cut(s) 54, 179, 459, 544
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 4 cut(s) 187, 463, 542, 631
BstMAI GTCTC 3 cut(s) 134, 287, 304
BstMBI GATC 4 cut(s) 184, 460, 539, 628
BstMWI GCNNNNNNNGC 2 cut(s) 165, 569
BstPAI GACNNNNGTC 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 267
BstUI CGCG 4 cut(s) 54, 179, 459, 544
BstV1I GCAGC 3 cut(s) 46, 235, 533
BstXI CCANNNNNNTGG 1 cut(s) 438
BsuRI GGCC 2 cut(s) 403, 416
BtrI CACGTC 1 cut(s) 297
BtsCI GGATG 1 cut(s) 223
BtsIMutI CAGTG 1 cut(s) 436
Cac8I GCNNGC 1 cut(s) 236
CciI TCATGA 1 cut(s) 208
CfoI GCGC 1 cut(s) 181
Cfr13I GGNCC 1 cut(s) 427
CseI GACGC 1 cut(s) 47
Csp6I GTAC 2 cut(s) 243, 264
CviAII CATG 4 cut(s) 68, 209, 391, 581
CviQI GTAC 2 cut(s) 243, 264
DdeI CTNAG 3 cut(s) 132, 342, 405
DpnI GATC 4 cut(s) 186, 462, 541, 630
DpnII GATC 4 cut(s) 184, 460, 539, 628
DraIII CACNNNGTG 1 cut(s) 135
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
Ecl136II GAGCTC 3 cut(s) 514, 552, 622
Eco130I CCWWGG 1 cut(s) 504
Eco24I GRGCYC 3 cut(s) 516, 554, 624
Eco47I GGWCC 1 cut(s) 427
Eco53kI GAGCTC 3 cut(s) 514, 552, 622
Eco72I CACGTG 1 cut(s) 146
EcoICRI GAGCTC 3 cut(s) 514, 552, 622
EcoO109I RGGNCCY 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 504
EcoT38I GRGCYC 3 cut(s) 516, 554, 624
ErhI CCWWGG 1 cut(s) 504
Esp3I CGTCTC 1 cut(s) 287
FaeI CATG 4 cut(s) 71, 212, 394, 584
FaqI GGGAC 2 cut(s) 134, 413
FatI CATG 4 cut(s) 67, 208, 390, 580
Fnu4HI GCNGC 3 cut(s) 60, 249, 522
FokI GGATG 1 cut(s) 230
FriOI GRGCYC 3 cut(s) 516, 554, 624
Fsp4HI GCNGC 3 cut(s) 60, 249, 522
FspBI CTAG 2 cut(s) 105, 564
GlaI GCGC 1 cut(s) 180
GluI GCNGC 3 cut(s) 60, 249, 522
GsuI CTGGAG 1 cut(s) 460
HaeIII GGCC 2 cut(s) 403, 416
HapII CCGG 3 cut(s) 269, 290, 307
HgaI GACGC 1 cut(s) 47
HhaI GCGC 1 cut(s) 181
Hin1II CATG 4 cut(s) 71, 212, 394, 584
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HindIII AAGCTT 1 cut(s) 472
HinfI GANTC 2 cut(s) 348, 587
HpaII CCGG 3 cut(s) 269, 290, 307
HphI GGTGA 1 cut(s) 500
Hpy188I TCNGA 4 cut(s) 29, 331, 531, 539
Hpy188III TCNNGA 1 cut(s) 209
Hpy99I CGWCG 3 cut(s) 41, 194, 629
HpyAV CCTTC 2 cut(s) 179, 418
HpyCH4III ACNGT 1 cut(s) 488
HpyCH4IV ACGT 3 cut(s) 20, 145, 296
HpyCH4V TGCA 2 cut(s) 62, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 165, 569
HpyF3I CTNAG 3 cut(s) 132, 342, 405
HpySE526I ACGT 3 cut(s) 20, 145, 296
Hsp92II CATG 4 cut(s) 71, 212, 394, 584
HspAI GCGC 1 cut(s) 179
KpnI GGTACC 1 cut(s) 267
Kzo9I GATC 4 cut(s) 184, 460, 539, 628
LguI GCTCTTC 1 cut(s) 504
LmnI GCTCC 5 cut(s) 56, 441, 448, 549, 619
LpnPI CCDG 8 cut(s) 173, 242, 282, 303, 320, 385, 417, 424
Lsp1109I GCAGC 3 cut(s) 46, 235, 533
LweI GCATC 1 cut(s) 612
MaeI CTAG 2 cut(s) 105, 564
MaeII ACGT 3 cut(s) 20, 145, 296
MaeIII GTNAC 2 cut(s) 302, 488
MalI GATC 4 cut(s) 186, 462, 541, 630
MboI GATC 4 cut(s) 184, 460, 539, 628
MboII GAAGA 2 cut(s) 95, 521
MhlI GDGCHC 3 cut(s) 516, 554, 624
MluCI AATT 3 cut(s) 30, 357, 432
MmeI TCCRAC 1 cut(s) 527
MnlI CCTC 6 cut(s) 73, 215, 221, 265, 268, 610
MseI TTAA 3 cut(s) 44, 164, 420
MspI CCGG 3 cut(s) 269, 290, 307
MspR9I CCNGG 1 cut(s) 269
MvnI CGCG 4 cut(s) 54, 179, 459, 544
MwoI GCNNNNNNNGC 2 cut(s) 165, 569
NciI CCSGG 1 cut(s) 269
NdeII GATC 4 cut(s) 184, 460, 539, 628
NlaIII CATG 4 cut(s) 71, 212, 394, 584
NlaIV GGNNCC 2 cut(s) 265, 429
NmuCI GTSAC 1 cut(s) 488
PagI TCATGA 1 cut(s) 208
PciSI GCTCTTC 1 cut(s) 504
PfeI GAWTC 2 cut(s) 348, 587
PflMI CCANNNNNTGG 1 cut(s) 135
PkrI GCNGC 3 cut(s) 61, 250, 523
PmaCI CACGTG 1 cut(s) 146
PmlI CACGTG 1 cut(s) 146
Ppu21I YACGTR 1 cut(s) 146
PpuMI RGGWCCY 1 cut(s) 427
PshAI GACNNNNGTC 1 cut(s) 146
PsiI TTATAA 2 cut(s) 635, 660
Psp124BI GAGCTC 3 cut(s) 516, 554, 624
Psp5II RGGWCCY 1 cut(s) 427
PspCI CACGTG 1 cut(s) 146
PspN4I GGNNCC 2 cut(s) 265, 429
PspPI GGNCC 1 cut(s) 427
PspPPI RGGWCCY 1 cut(s) 427
RsaI GTAC 2 cut(s) 244, 265
RsaNI GTAC 2 cut(s) 243, 264
SacI GAGCTC 3 cut(s) 516, 554, 624
SapI GCTCTTC 1 cut(s) 504
SaqAI TTAA 3 cut(s) 44, 164, 420
SatI GCNGC 3 cut(s) 60, 249, 522
Sau3AI GATC 4 cut(s) 184, 460, 539, 628
Sau96I GGNCC 1 cut(s) 427
ScaI AGTACT 1 cut(s) 244
ScrFI CCNGG 1 cut(s) 269
SduI GDGCHC 3 cut(s) 516, 554, 624
SfaNI GCATC 1 cut(s) 612
SinI GGWCC 1 cut(s) 427
SmlI CTYRAG 1 cut(s) 515
SmoI CTYRAG 1 cut(s) 515
Sse9I AATT 3 cut(s) 30, 357, 432
SsiI CCGC 2 cut(s) 54, 457
SspMI CTAG 2 cut(s) 105, 564
SstI GAGCTC 3 cut(s) 516, 554, 624
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 1 cut(s) 488
TaiI ACGT 3 cut(s) 23, 148, 299
TaqI TCGA 2 cut(s) 183, 624
TasI AATT 3 cut(s) 30, 357, 432
TatI WGTACW 1 cut(s) 242
TfiI GAWTC 2 cut(s) 348, 587
Tru1I TTAA 3 cut(s) 44, 164, 420
Tru9I TTAA 3 cut(s) 44, 164, 420
TscAI CASTG 1 cut(s) 443
TseFI GTSAC 1 cut(s) 488
TseI GCWGC 3 cut(s) 59, 248, 521
Tsp45I GTSAC 1 cut(s) 488
TspDTI ATGAA 1 cut(s) 197
TspGWI ACGGA 1 cut(s) 507
TspRI CASTG 1 cut(s) 443
Van91I CCANNNNNTGG 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 427
XspI CTAG 2 cut(s) 105, 564
ZrmI AGTACT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.