pycom06g21560

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0004447
Physical Location & Seq
Forward (+)
24156504 .. 24161025
4522 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1284 bp
ATGGGATGCCTGGTCTCAACACCGAAAGATACACTAGGAAATAGGAGAAGGCCCGCAAACATTGGGGAAGTCTCTGTCTATGTCCCTGGTTTGCGAATCCCTAAACCTGTTGATTTCTCTCAGTCACTTGGTGATCACTTGTCCAAGAGTTTAGTGGAACGCCTATCTGCATTAAGGACTCGCATAGTTGTGATGGCTGCCCAAGAAGGTCCGACAATTACCAGAACGAAGAGAAGAGCTGCTACTCAACATGGTTTGTGGTTCAACATTGGCCGATCGATCTTTGATTGTATCTTTATTCAGCAGGCTCTCGAGGATTACTTGCCTGTCCTTTTGGGATTAATGAAAGATGGAAGCCAGCTACACCACAAGGTTCAATTTTTGTGGGTAAATCAAGAGGATGATGCAGAGGAAACAGCTATGTCTAACGCATGGTACGAGGTGCTGTCGATTTTGCACTTAATGGCAATGCTAGCATTATCACAAGCTAACTTGTTACTTCTTCCAAGAACATCTGCTGATGGTTATCAGCCAAAAGTAACAGAAGAGAGTAGACGAGCTTCCATTGATATTTTCCTGAAGGCAGCAGGATACCTTGATTGTGCAGTCCGACATGTTCTTCCACAGTTGCCTACTGAGCTCAAGACAAACCTGCCAGTTGACTTAGCAGAAGGAGTTCTTCGAGCGCTTTGCCTACAAGCATTAGGACAGGGTGTTGATATTCAACTTGGAATGGCAATTGACAGCACAAAAGCCACACTTGCAGTGAAGCGAAGGCTTGCATGTGAAATGGTAAAGTATTGGCAACAGGCTCAAGATAACCTTATGAACCTTCCCTTATTAAATGGTTGGGGTGAAAAGCATCGGCTCTTTGTGAAGTGGAAATATGTTGAGGCAAAGGCTGCTGCATATTACTATCATGGTTTGATCCTTGATGAGGGTAACACAGAAAAATTTCATGGGATGGCTGTAGCTGCCTTGCAAGCAGCAGATGAGTATTTTAAAGAAAGTAGGAAGGCATGCGACGCATTTAACGCTGCTCCCCCTTTGTCGAGAAATCCACCGCTCTGGGGAACCATGAAGTATCTATCTGAGAAAATTCCTAAAGATACTTCAAGCAAAGTGCGGATAAACCGTGATCTGTACTCACACGAAAAAATCATGGAGACAGCGCCAACATTGCCCGATTTTGCATTGGCCTTGAAACCGGATGAATTCCAACTTCCTCCAGTAGACCCCTCTTGGAACATGGAGCACATGAATAAGGGTTCCTACCAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

428

Amino Acids

47.96

Weight (kDa)

8.1

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 122 - 342 5.1e-13 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 660
AccBSI CCGCTC 1 cut(s) 1066
AccI GTMKAC 2 cut(s) 553, 1233
AciI CCGC 3 cut(s) 54, 1064, 1126
AclWI GGATC 1 cut(s) 922
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 3 cut(s) 953, 1098, 1214
AcuI CTGAAG 1 cut(s) 599
AdeI CACNNNGTG 1 cut(s) 131
AfaI GTAC 2 cut(s) 437, 1145
AfeI AGCGCT 1 cut(s) 687
AfiI CCNNNNNNNGG 2 cut(s) 937, 1070
AflIII ACRYGT 1 cut(s) 613
AgsI TTSAA 5 cut(s) 265, 377, 725, 1116, 1204
AjnI CCWGG 2 cut(s) 9, 85
AluBI AGCT 8 cut(s) 239, 361, 419, 488, 560, 640, 974, 1279
AluI AGCT 8 cut(s) 239, 361, 419, 488, 560, 640, 974, 1279
Alw21I GWGCWC 2 cut(s) 642, 1257
Alw26I GTCTC 3 cut(s) 19, 76, 1160
AlwI GGATC 1 cut(s) 922
Ama87I CYCGRG 1 cut(s) 311
Aor51HI AGCGCT 1 cut(s) 687
AoxI GGCC 3 cut(s) 50, 271, 1197
ApeKI GCWGC 8 cut(s) 197, 239, 584, 902, 905, 974, 986, 1037
ApoI RAATTY 3 cut(s) 953, 1098, 1214
AseI ATTAAT 1 cut(s) 341
Asp700I GAANNNNTTC 2 cut(s) 675, 954
AspLEI GCGC 2 cut(s) 688, 1174
AspS9I GGNCC 2 cut(s) 51, 209
AsuHPI GGTGA 2 cut(s) 143, 866
AsuNHI GCTAGC 1 cut(s) 472
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 1 cut(s) 209
BanII GRGCYC 1 cut(s) 642
BarI GAAGNNNNNNTAC 4 cut(s) 226, 258, 544, 576
Bbv12I GWGCWC 2 cut(s) 642, 1257
BbvI GCAGC 8 cut(s) 184, 226, 596, 889, 892, 961, 998, 1024
BccI CCATC 4 cut(s) 187, 344, 515, 958
BcgI CGANNNNNNTGC 2 cut(s) 672, 706
BciT130I CCWGG 2 cut(s) 11, 87
BciVI GTATCC 1 cut(s) 584
BclI TGATCA 1 cut(s) 133
BcoDI GTCTC 3 cut(s) 19, 76, 1160
BfaI CTAG 2 cut(s) 35, 473
BfmI CTRYAG 1 cut(s) 969
BfoI RGCGCY 2 cut(s) 689, 1175
BfuAI ACCTGC 1 cut(s) 660
BfuI GTATCC 1 cut(s) 584
BisI GCNGC 8 cut(s) 198, 240, 585, 903, 906, 975, 987, 1038
BlsI GCNGC 8 cut(s) 199, 241, 586, 904, 907, 976, 988, 1039
Bme1390I CCNGG 2 cut(s) 11, 87
Bme18I GGWCC 1 cut(s) 209
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 51, 209
BmiI GGNNCC 2 cut(s) 1075, 1270
BmrFI CCNGG 2 cut(s) 11, 87
BmsI GCATC 2 cut(s) 394, 871
BmtI GCTAGC 1 cut(s) 476
BpmI CTGGAG 1 cut(s) 1212
BpuEI CTTGAG 2 cut(s) 626, 798
Bsa29I ATCGAT 1 cut(s) 278
BsaBI GATNNNNATC 1 cut(s) 525
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 1 cut(s) 85
BsaWI WCCGGW 1 cut(s) 1207
Bsc4I CCNNNNNNNGG 2 cut(s) 937, 1070
Bse1I ACTGG 2 cut(s) 656, 1229
Bse3DI GCAATG 2 cut(s) 474, 1178
Bse8I GATNNNNATC 1 cut(s) 525
BseBI CCWGG 2 cut(s) 11, 87
BseCI ATCGAT 1 cut(s) 278
BseDI CCNNGG 1 cut(s) 85
BseGI GGATG 4 cut(s) 11, 406, 969, 1216
BseJI GATNNNNATC 1 cut(s) 525
BseLI CCNNNNNNNGG 2 cut(s) 937, 1070
BseMI GCAATG 2 cut(s) 474, 1178
BseMII CTCAG 3 cut(s) 134, 627, 1083
BseNI ACTGG 2 cut(s) 656, 1229
BseXI GCAGC 8 cut(s) 184, 226, 596, 889, 892, 961, 998, 1024
BsgI GTGCAG 1 cut(s) 624
Bsh1285I CGRYCG 1 cut(s) 278
BshFI GGCC 3 cut(s) 52, 273, 1199
BshVI ATCGAT 1 cut(s) 278
BsiEI CGRYCG 1 cut(s) 278
BsiHKAI GWGCWC 2 cut(s) 642, 1257
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 1 cut(s) 1208
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 2 cut(s) 937, 1070
BsmAI GTCTC 3 cut(s) 19, 76, 1160
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 3 cut(s) 52, 273, 1199
Bso31I GGTCTC 1 cut(s) 19
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 642, 1257
Bsp143I GATC 5 cut(s) 133, 275, 279, 927, 1138
BspACI CCGC 3 cut(s) 54, 1064, 1126
BspANI GGCC 3 cut(s) 52, 273, 1199
BspCNI CTCAG 3 cut(s) 133, 628, 1084
BspDI ATCGAT 1 cut(s) 278
BspLI GGNNCC 2 cut(s) 1075, 1270
BspMI ACCTGC 1 cut(s) 660
BspOI GCTAGC 1 cut(s) 476
BspPI GGATC 1 cut(s) 922
BspQI GCTCTTC 1 cut(s) 229
BspTNI GGTCTC 1 cut(s) 19
BsrBI CCGCTC 1 cut(s) 1066
BsrDI GCAATG 2 cut(s) 474, 1178
BsrI ACTGG 2 cut(s) 656, 1229
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 5 cut(s) 133, 275, 279, 927, 1138
Bst2UI CCWGG 2 cut(s) 11, 87
Bst4CI ACNGT 2 cut(s) 627, 1136
Bst6I CTCTTC 3 cut(s) 224, 229, 540
BstAPI GCANNNNNTGC 1 cut(s) 902
BstC8I GCNNGC 7 cut(s) 54, 306, 359, 474, 780, 984, 1021
BstDEI CTNAG 4 cut(s) 120, 636, 664, 1092
BstENI CCTNNNNNAGG 1 cut(s) 935
BstF5I GGATG 4 cut(s) 11, 406, 969, 1216
BstH2I RGCGCY 2 cut(s) 689, 1175
BstHHI GCGC 2 cut(s) 688, 1174
BstKTI GATC 5 cut(s) 136, 278, 282, 930, 1141
BstMAI GTCTC 3 cut(s) 19, 76, 1160
BstMBI GATC 5 cut(s) 133, 275, 279, 927, 1138
BstMCI CGRYCG 1 cut(s) 278
BstMWI GCNNNNNNNGC 9 cut(s) 473, 637, 761, 902, 974, 983, 1025, 1034, 1180
BstNI CCWGG 2 cut(s) 11, 87
BstNSI RCATGY 3 cut(s) 617, 786, 1023
BstSCI CCNGG 2 cut(s) 9, 85
BstSFI CTRYAG 1 cut(s) 969
BstV1I GCAGC 8 cut(s) 184, 226, 596, 889, 892, 961, 998, 1024
BstXI CCANNNNNNTGG 1 cut(s) 1068
Bsu15I ATCGAT 1 cut(s) 278
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 3 cut(s) 52, 273, 1199
BsuTUI ATCGAT 1 cut(s) 278
BtsCI GGATG 4 cut(s) 11, 406, 969, 1216
BtsI GCAGTG 1 cut(s) 771
BtsIMutI CAGTG 1 cut(s) 771
BveI ACCTGC 1 cut(s) 660
Cac8I GCNNGC 7 cut(s) 54, 306, 359, 474, 780, 984, 1021
CfoI GCGC 2 cut(s) 688, 1174
Cfr13I GGNCC 2 cut(s) 51, 209
ClaI ATCGAT 1 cut(s) 278
CseI GACGC 1 cut(s) 1034
Csp6I GTAC 2 cut(s) 436, 1144
CspCI CAANNNNNGTGG 2 cut(s) 365, 400
CviQI GTAC 2 cut(s) 436, 1144
DdeI CTNAG 4 cut(s) 120, 636, 664, 1092
DpnI GATC 5 cut(s) 135, 277, 281, 929, 1140
DpnII GATC 5 cut(s) 133, 275, 279, 927, 1138
DraI TTTAAA 1 cut(s) 1003
DraIII CACNNNGTG 1 cut(s) 131
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 3 cut(s) 224, 229, 540
EarI CTCTTC 3 cut(s) 224, 229, 540
Ecl136II GAGCTC 1 cut(s) 640
Eco24I GRGCYC 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 209
Eco47III AGCGCT 1 cut(s) 687
Eco53kI GAGCTC 1 cut(s) 640
Eco57I CTGAAG 1 cut(s) 599
Eco88I CYCGRG 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 640
EcoNI CCTNNNNNAGG 1 cut(s) 935
EcoRI GAATTC 1 cut(s) 1214
EcoRII CCWGG 2 cut(s) 9, 85
EcoT38I GRGCYC 1 cut(s) 642
FalI AAGNNNNNCTT 6 cut(s) 663, 695, 744, 776, 807, 839
FaqI GGGAC 1 cut(s) 68
FauI CCCGC 1 cut(s) 61
FbaI TGATCA 1 cut(s) 133
FblI GTMKAC 2 cut(s) 553, 1233
Fnu4HI GCNGC 8 cut(s) 198, 240, 585, 903, 906, 975, 987, 1038
FokI GGATG 4 cut(s) 18, 413, 976, 1223
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 8 cut(s) 198, 240, 585, 903, 906, 975, 987, 1038
FspBI CTAG 2 cut(s) 35, 473
GlaI GCGC 2 cut(s) 687, 1173
GluI GCNGC 8 cut(s) 198, 240, 585, 903, 906, 975, 987, 1038
GsuI CTGGAG 1 cut(s) 1212
HaeII RGCGCY 2 cut(s) 689, 1175
HaeIII GGCC 3 cut(s) 52, 273, 1199
HapII CCGG 1 cut(s) 1208
HgaI GACGC 1 cut(s) 1034
HhaI GCGC 2 cut(s) 688, 1174
Hin6I GCGC 2 cut(s) 686, 1172
HinP1I GCGC 2 cut(s) 686, 1172
HincII GTYRAC 1 cut(s) 661
HindII GTYRAC 1 cut(s) 661
HinfI GANTC 2 cut(s) 96, 178
HpaII CCGG 1 cut(s) 1208
HphI GGTGA 2 cut(s) 143, 866
Hpy166II GTNNAC 3 cut(s) 554, 661, 1234
Hpy188I TCNGA 3 cut(s) 213, 611, 1093
Hpy188III TCNNGA 6 cut(s) 311, 395, 577, 643, 815, 1053
Hpy8I GTNNAC 3 cut(s) 554, 661, 1234
Hpy99I CGWCG 1 cut(s) 1028
HpyAV CCTTC 7 cut(s) 42, 200, 574, 665, 768, 842, 1009
HpyCH4III ACNGT 2 cut(s) 627, 1136
HpyCH4V TGCA 9 cut(s) 170, 407, 457, 605, 764, 782, 908, 982, 1193
HpyF10VI GCNNNNNNNGC 9 cut(s) 473, 637, 761, 902, 974, 983, 1025, 1034, 1180
HpyF3I CTNAG 4 cut(s) 120, 636, 664, 1092
HspAI GCGC 2 cut(s) 686, 1172
Ksp22I TGATCA 1 cut(s) 133
Kzo9I GATC 5 cut(s) 133, 275, 279, 927, 1138
LguI GCTCTTC 1 cut(s) 229
LmnI GCTCC 2 cut(s) 1045, 1252
Lsp1109I GCAGC 8 cut(s) 184, 226, 596, 889, 892, 961, 998, 1024
LweI GCATC 2 cut(s) 394, 871
MaeI CTAG 2 cut(s) 35, 473
MaeIII GTNAC 4 cut(s) 123, 495, 538, 941
MalI GATC 5 cut(s) 135, 277, 281, 929, 1140
MbiI CCGCTC 1 cut(s) 1066
MboI GATC 5 cut(s) 133, 275, 279, 927, 1138
MboII GAAGA 6 cut(s) 241, 246, 494, 557, 611, 671
MfeI CAATTG 1 cut(s) 738
MhlI GDGCHC 2 cut(s) 642, 1257
MluCI AATT 6 cut(s) 216, 377, 738, 953, 1098, 1214
MlyI GAGTC 1 cut(s) 172
MmeI TCCRAC 3 cut(s) 236, 634, 1243
MnlI CCTC 8 cut(s) 307, 391, 403, 433, 886, 931, 1236, 1249
MroXI GAANNNNTTC 2 cut(s) 675, 954
MseI TTAA 6 cut(s) 173, 341, 461, 842, 1002, 1032
MslI CAYNNNNRTG 1 cut(s) 188
MspI CCGG 1 cut(s) 1208
MspR9I CCNGG 2 cut(s) 11, 87
MunI CAATTG 1 cut(s) 738
MvaI CCWGG 2 cut(s) 11, 87
MwoI GCNNNNNNNGC 9 cut(s) 473, 637, 761, 902, 974, 983, 1025, 1034, 1180
NdeII GATC 5 cut(s) 133, 275, 279, 927, 1138
NheI GCTAGC 1 cut(s) 472
NlaIV GGNNCC 2 cut(s) 1075, 1270
NmuCI GTSAC 1 cut(s) 123
NspI RCATGY 3 cut(s) 617, 786, 1023
PaeI GCATGC 1 cut(s) 1023
PaeR7I CTCGAG 1 cut(s) 311
PciI ACATGT 1 cut(s) 613
PciSI GCTCTTC 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PdmI GAANNNNTTC 2 cut(s) 675, 954
PfeI GAWTC 1 cut(s) 96
PkrI GCNGC 8 cut(s) 199, 241, 586, 904, 907, 976, 988, 1039
Ple19I CGATCG 1 cut(s) 278
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PscI ACATGT 1 cut(s) 613
PshBI ATTAAT 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 642
Psp6I CCWGG 2 cut(s) 9, 85
PspGI CCWGG 2 cut(s) 9, 85
PspN4I GGNNCC 2 cut(s) 1075, 1270
PspPI GGNCC 2 cut(s) 51, 209
PvuI CGATCG 1 cut(s) 278
RsaI GTAC 2 cut(s) 437, 1145
RsaNI GTAC 2 cut(s) 436, 1144
RseI CAYNNNNRTG 1 cut(s) 188
SacI GAGCTC 1 cut(s) 642
SapI GCTCTTC 1 cut(s) 229
SaqAI TTAA 6 cut(s) 173, 341, 461, 842, 1002, 1032
SatI GCNGC 8 cut(s) 198, 240, 585, 903, 906, 975, 987, 1038
Sau3AI GATC 5 cut(s) 133, 275, 279, 927, 1138
Sau96I GGNCC 2 cut(s) 51, 209
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 2 cut(s) 11, 87
SduI GDGCHC 2 cut(s) 642, 1257
SfaNI GCATC 2 cut(s) 394, 871
SfcI CTRYAG 1 cut(s) 969
Sfr274I CTCGAG 1 cut(s) 311
SinI GGWCC 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 311
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 3 cut(s) 311, 641, 813
SmoI CTYRAG 3 cut(s) 311, 641, 813
SphI GCATGC 1 cut(s) 1023
Sse9I AATT 6 cut(s) 216, 377, 738, 953, 1098, 1214
SsiI CCGC 3 cut(s) 54, 1064, 1126
SspMI CTAG 2 cut(s) 35, 473
SstI GAGCTC 1 cut(s) 642
StyD4I CCNGG 2 cut(s) 9, 85
TaaI ACNGT 2 cut(s) 627, 1136
TaqI TCGA 5 cut(s) 278, 312, 449, 682, 1052
TasI AATT 6 cut(s) 216, 377, 738, 953, 1098, 1214
TatI WGTACW 1 cut(s) 1143
TfiI GAWTC 1 cut(s) 96
Tru1I TTAA 6 cut(s) 173, 341, 461, 842, 1002, 1032
Tru9I TTAA 6 cut(s) 173, 341, 461, 842, 1002, 1032
TscAI CASTG 1 cut(s) 771
TseFI GTSAC 1 cut(s) 123
TseI GCWGC 8 cut(s) 197, 239, 584, 902, 905, 974, 986, 1037
Tsp45I GTSAC 1 cut(s) 123
TspDTI ATGAA 6 cut(s) 359, 842, 947, 1094, 1227, 1274
TspRI CASTG 1 cut(s) 771
VpaK11BI GGWCC 1 cut(s) 209
VspI ATTAAT 1 cut(s) 341
XagI CCTNNNNNAGG 1 cut(s) 935
XapI RAATTY 3 cut(s) 953, 1098, 1214
XceI RCATGY 3 cut(s) 617, 786, 1023
XcmI CCANNNNNNNNNTGG 1 cut(s) 151
XhoI CTCGAG 1 cut(s) 311
XmiI GTMKAC 2 cut(s) 553, 1233
XmnI GAANNNNTTC 2 cut(s) 675, 954
XspI CTAG 2 cut(s) 35, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.