pycom07g00500

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010834
Physical Location & Seq
Reverse (-)
476670 .. 477065
396 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g00500.1

Sequence Viewer

Length: 396 bp
ATGCTGGTCTATGACAAAACCAAGCCTCAATCATTTGATCATGTAACCAAGTGGTTAGAGGAATTGAGGGCGCATGCCGACAACAATATTGTTGTGATGCTTGTAGGCAACAAGTCCGACCTGGGGACACTGCGAGCTGTACCTTCTGAGGATGCGAAAGAGTTTGCCCAAAGGGAGAACCTCTTCTTTATGGAGGCATCGGCACTTGAGGCCACTAATGTTGAATCTGCATTCGTTACTGTCCTAACAGAAATTTATCGAATCCTCGGCAAGAAGTCCCTCATTCCAAATGAGGAAGCAGAGTCTGGGAGTTCCTCACTTCTCAAGGGAACAAAACTTGTTGTTCCTGGACAAGAGCCTGAGCCTCAAGCGAGTAGCTGTTGCTGGTCTTCATAA

Protein Analysis

132

Amino Acids

14.45

Weight (kDa)

4.92

Isoelectric Point (pI)

50.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ras PF00071 1 - 86 6.3e-25 Ras family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 252
AfaI GTAC 1 cut(s) 141
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 1 cut(s) 224
AjnI CCWGG 2 cut(s) 120, 346
AluBI AGCT 2 cut(s) 137, 378
AluI AGCT 2 cut(s) 137, 378
AlwNI CAGNNNCTG 1 cut(s) 305
AoxI GGCC 1 cut(s) 210
ApoI RAATTY 1 cut(s) 252
Asp700I GAANNNNTTC 1 cut(s) 182
AspLEI GCGC 1 cut(s) 73
BbsI GAAGAC 1 cut(s) 381
BciT130I CCWGG 2 cut(s) 122, 348
BclI TGATCA 1 cut(s) 37
Bme1390I CCNGG 2 cut(s) 122, 348
BmrFI CCNGG 2 cut(s) 122, 348
BmsI GCATC 3 cut(s) 87, 142, 206
BpiI GAAGAC 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 360
BpuEI CTTGAG 3 cut(s) 227, 308, 351
BsaJI CCNNGG 2 cut(s) 121, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 123
BseBI CCWGG 2 cut(s) 122, 348
BseDI CCNNGG 2 cut(s) 121, 265
BseGI GGATG 1 cut(s) 157
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMII CTCAG 2 cut(s) 138, 351
BshFI GGCC 1 cut(s) 212
BslFI GGGAC 2 cut(s) 139, 262
BslI CCNNNNNNNGG 1 cut(s) 123
BsmFI GGGAC 2 cut(s) 139, 262
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 1 cut(s) 212
Bsp143I GATC 1 cut(s) 37
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 2 cut(s) 139, 352
BssECI CCNNGG 2 cut(s) 121, 265
BssMI GATC 1 cut(s) 37
Bst2UI CCWGG 2 cut(s) 122, 348
Bst4CI ACNGT 1 cut(s) 241
Bst6I CTCTTC 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 75, 135
BstDEI CTNAG 2 cut(s) 147, 360
BstF5I GGATG 1 cut(s) 157
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 1 cut(s) 40
BstMBI GATC 1 cut(s) 37
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 2 cut(s) 122, 348
BstNSI RCATGY 1 cut(s) 77
BstSCI CCNGG 2 cut(s) 120, 346
BstV2I GAAGAC 1 cut(s) 381
BsuRI GGCC 1 cut(s) 212
BtsCI GGATG 1 cut(s) 157
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 128
Cac8I GCNNGC 2 cut(s) 75, 135
CaiI CAGNNNCTG 1 cut(s) 305
CfoI GCGC 1 cut(s) 73
Csp6I GTAC 1 cut(s) 140
CspCI CAANNNNNGTGG 2 cut(s) 202, 237
CviAII CATG 2 cut(s) 41, 74
CviJI RGCY 6 cut(s) 25, 137, 212, 358, 364, 378
CviKI_1 RGCY 6 cut(s) 25, 137, 212, 358, 364, 378
CviQI GTAC 1 cut(s) 140
DdeI CTNAG 2 cut(s) 147, 360
DpnI GATC 1 cut(s) 39
DpnII GATC 1 cut(s) 37
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EcoRII CCWGG 2 cut(s) 120, 346
FaeI CATG 2 cut(s) 44, 77
FaiI YATR 5 cut(s) 12, 42, 75, 191, 394
FaqI GGGAC 2 cut(s) 139, 262
FatI CATG 2 cut(s) 40, 73
FbaI TGATCA 1 cut(s) 37
FokI GGATG 1 cut(s) 164
GlaI GCGC 1 cut(s) 72
HaeIII GGCC 1 cut(s) 212
HhaI GCGC 1 cut(s) 73
Hin1II CATG 2 cut(s) 44, 77
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HinfI GANTC 3 cut(s) 224, 261, 302
Hpy188I TCNGA 2 cut(s) 118, 148
HpyAV CCTTC 1 cut(s) 153
HpyCH4III ACNGT 1 cut(s) 241
HpyCH4V TGCA 1 cut(s) 230
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 2 cut(s) 147, 360
Hsp92II CATG 2 cut(s) 44, 77
HspAI GCGC 1 cut(s) 71
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 1 cut(s) 37
LpnPI CCDG 7 cut(s) 107, 134, 291, 333, 360, 370, 372
LweI GCATC 3 cut(s) 87, 142, 206
MaeIII GTNAC 2 cut(s) 43, 235
MalI GATC 1 cut(s) 39
MboI GATC 1 cut(s) 37
MboII GAAGA 2 cut(s) 175, 381
MluCI AATT 2 cut(s) 62, 252
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 182
MspR9I CCNGG 2 cut(s) 122, 348
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 2 cut(s) 122, 348
MwoI GCNNNNNNNGC 1 cut(s) 209
NdeII GATC 1 cut(s) 37
NlaIII CATG 2 cut(s) 44, 77
NmeAIII GCCGAG 1 cut(s) 246
NspI RCATGY 1 cut(s) 77
PaeI GCATGC 1 cut(s) 77
PctI GAATGC 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 182
PfeI GAWTC 2 cut(s) 224, 261
PfoI TCCNGGA 1 cut(s) 346
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
Psp6I CCWGG 2 cut(s) 120, 346
PspGI CCWGG 2 cut(s) 120, 346
PstNI CAGNNNCTG 1 cut(s) 305
RsaI GTAC 1 cut(s) 141
RsaNI GTAC 1 cut(s) 140
Sau3AI GATC 1 cut(s) 37
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 2 cut(s) 122, 348
SetI ASST 5 cut(s) 123, 139, 145, 183, 380
SfaNI GCATC 3 cut(s) 87, 142, 206
SmlI CTYRAG 3 cut(s) 206, 323, 366
SmoI CTYRAG 3 cut(s) 206, 323, 366
SphI GCATGC 1 cut(s) 77
Sse9I AATT 2 cut(s) 62, 252
SspI AATATT 1 cut(s) 88
StyD4I CCNGG 2 cut(s) 120, 346
TaaI ACNGT 1 cut(s) 241
TaqI TCGA 1 cut(s) 259
TasI AATT 2 cut(s) 62, 252
TfiI GAWTC 2 cut(s) 224, 261
TscAI CASTG 1 cut(s) 135
TspDTI ATGAA 1 cut(s) 381
TspRI CASTG 1 cut(s) 135
XapI RAATTY 1 cut(s) 252
XceI RCATGY 1 cut(s) 77
XmnI GAANNNNTTC 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.