pycom07g01980

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0015798
Physical Location & Seq
Forward (+)
1594695 .. 1598261
3567 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2847 bp
ATGCTGGTTTTGGCATTGATCGATCCTGAAGAAATTGCATCCTTTGCTCGATCTCATGATACGAAGGGTTTGGAAGGACACGGAGGAGTATCAGGATTAGCAGGAGAGGTTTCTGTATCGCTGAAAGATGGTGTTGTTTCAAGCAAGATACCTATTAGGCAGAATATATTTGGCCTAAACCAATATGCAGAGAAACCTTCTAAAGGTTTCTTGATGTTTCTCTGGGAAGCCTTACAAGATTTAACTTTAATTATCCTCATGGTTGCTGCTGCGGTCTCTATAGGTGTTGGAATTGCAACTGAAGGAATGCCGAAAGGTATGTATGATGGCTTGGGAATAATAGTTAGCATATTGTTAGTAGTGTTGGTGACTGCAATTAGCGACTACAAGCAGTCACTGCAATTTAAAGAACTGGACAAGGAGAAGAAAAACATAATGGTTCAGGTGACTAGAGATGGACGTAGACAGAAGGTCTCTATCTACGATTTGGTGGTTGGAGAAATTGTTCATCTTTCCATCGGTGATCAAGTTCCAGCTGATGGTATTTTCATATCAGGCCACAGCTTACAAGTCGACGAATCAAGCTTGTCAGGCGAGAGTGAGCCAGTGGACATAAATCAAGACAGACCTTTTCTTCTTGCAGGAACCAAAGTGCAAGATGGTTCTGGTAAAATGCTAGTGACATCAGTTGGTATGAGGACCGAATGGGGACGACTAATGGTTACTCTGAGTGAAGGGGGAGACAATGAGACACCACTGCAGGTGAAACTTAATGGAGTTGCAACCATTATCGGAAAAATTGGTTTGGCTTTTGCGGTGCTGACATTTATAGTTCTGATTTCAAGGTTTTTAGTAAACAAGGCGGTTCACCATCACATGAAAGACTGGTCTTCCAAAGATGCACAAAAGCTTTTGAATTACTTTTCGACTGCCGTCATTATAATTGTTGTTGCAGTCCCAGAAGGGCTACCTTTGGCAGTGACGTTAAGTCTTGCCTTTGCAATGAAAAAACTCATGTCGGATAAAGCACTTGTGAGGCATCTCTCTGCATGTGAGACAATGGGTTCTGCTACTTGTATTTGCACAGATAAAACAGGAACCTTGACAACAAATCATATGGTTGTGAACAAGATATGGGTCTGTAACGAAACCAAGACCATAACGAGTAATGAGAACAATGATGAGTTGAAATCGGCTTCTGAAGGAGTACATAACCTCCTTTTACAGTCCATCTTTCTGAATACAAGCGCGGAGGTAGTCAAGGGGGAAGATGGAAATAATAGCGTTATAGGGACTCCAACAGAGACGGCGATAATTGAATTTGGAATGCTTCTGGGAGGTGATTTTAAATCTTACAGTGAGGATTATAAGATACTTACGGTTGAACCATTCAATTCAGTCAGGAAAAAGATGTCGGTGCTAGCGGCTCTACCCGATGGAGGAAAGAAACGAGCATTCTGCAAAGGAGCATCAGAAATAATCTTAGAAATGTGTGATAATGTTGTTGGCACTGATGGTGAAGCTGTGCCTCTATCGGACGAGCAAAGAACGAATATCTCTGATATCATAAATGGTTTTGCATGTGAAGCTTTAAGAACTCTATGCTTAGCTTTTAAGAATATAGATGATTCCTCTAGTGAAAGCAGTATTCCTGACGATAAGTATACGTTAATAGCTGTTGTTGGAATCAAGGATCCTGTGCGTCCAGGGGTGAGGGAAGCCGTGAAGACTTGTTTAGATGCTGGGATTACTGTAAGGATGGTCACCGGTGACAATATTAATACGGCTAAAGCCATAGCTACGGAGTGTGGTATTTTGACAGAAGGTGGTTTGGCTATAGAAGGGCCAGATTTCCGAAACAAGAGCTCACAGGAGATGGCGGAAATAATTCCGAAACTGCAGGTAATGGCTCGATCCTTGCCCTTGGACAAGCACACCTTGGTGAAACAGTTGAGGAATGAGCTTAGAGAAGTTGTGGCAGTAACTGGTGATGGGACAAATGACGCTCCTGCGCTGCACGAGGCAGACATCGGACTTGCCATGGGTATTGCGGGAACGGAGGTTGCGAAAGAGAATGCCGATGTTATCATAATGGATGACAACTTCACAAGCATAGTGAACGTTGCAAGATGGGGTCGGGCGGTTTACATCAACATTCAAAAGTTTGTACAGTTCCAACTAACAGTTAACATCGCAGCTCTGATGCTCAATTTCGTTTCAGCATGCGTCTCAGGATCCGCGCCTCTCACGGCTGTCCAGATGCTTTGGGTGAACTTGATCATGGATACTCTCGGTGCTTTGGCGCTGGCTACGGAGCCACCAAACGAGGGCCTAATGAAAAGACCACCGGTCGGAAGGAACGTCAAGTTCATAACCGGGATCATGTGGAGGAACATTGTTGGCCAGAGTATCTACCAAATTGCTGTCCTTCTGGTTCTCAAGTTTTGTGGAATCCGGCTTTTGAAGCTAACTGGTTCCGACGCAAATTCCGTTCTCAATACCGTCATATTCAACTCTTTTGTGTTTTGCCAGGCATTCAACGAGATAAACAGCCGTGATATGGAGAAGATCGATGTGTTCAGTGGCATGTTCAGCAGTTACACCTTCATGATGGTGATGATCTCCACCGTTGTTTTCCAGATCATCATCGTTGAATTCTTGGGCAAGTTTGCACAAACCGTGCCACTGAGCTGGGAGTTGTGGTTAACAAGCGTCTTGATCGGCGCTGTAAGTTTGCTCGTTGCTGTCGTCCTCAAGTTCATTCCTGTTTCAGTCAGGAAACAACAAGCTCCTCGGGATCACGAGGATGTCTACGAACCTCTCCTCAGTGGCCCTGACAACGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

949

Amino Acids

102.63

Weight (kDa)

5.3

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 27 - 93 9.8e-11 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 145 - 240 3.9e-22 P-type ATPase actuator domain
Cation_ATPase PF13246 407 - 569 4e-35 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 554 - 676 6.9e-12 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 747 - 921 5.8e-41 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 941, 1368
AarI CACCTGC 1 cut(s) 751
Acc36I ACCTGC 2 cut(s) 751, 1891
AccB7I CCANNNNNTGG 1 cut(s) 539
AccI GTMKAC 4 cut(s) 463, 573, 1664, 2811
AccII CGCG 2 cut(s) 1250, 2240
AciI CCGC 9 cut(s) 272, 815, 863, 1250, 1424, 1880, 2051, 2141, 2238
AclI AACGTT 1 cut(s) 2121
AclWI GGATC 8 cut(s) 17, 1688, 1701, 1908, 2229, 2242, 2387, 2805
AcoI YGGCCR 1 cut(s) 2401
AcsI RAATTY 3 cut(s) 1319, 2485, 2654
AcuI CTGAAG 3 cut(s) 48, 321, 1221
AfaI GTAC 2 cut(s) 1209, 2169
AfiI CCNNNNNNNGG 6 cut(s) 203, 539, 1439, 2327, 2351, 2560
AgeI ACCGGT 2 cut(s) 1766, 2347
AhdI GACNNNNNGTC 3 cut(s) 470, 987, 2348
AjnI CCWGG 2 cut(s) 1705, 2529
AleI CACNNNNGTG 1 cut(s) 1940
AloI GAACNNNNNNTCC 2 cut(s) 489, 521
Alw21I GWGCWC 1 cut(s) 1868
Alw26I GTCTC 7 cut(s) 280, 478, 735, 743, 1049, 1298, 2233
AlwI GGATC 8 cut(s) 17, 1688, 1701, 1908, 2229, 2242, 2387, 2805
AlwNI CAGNNNCTG 2 cut(s) 397, 1985
Ama87I CYCGRG 1 cut(s) 2793
AoxI GGCC 6 cut(s) 172, 556, 1844, 2329, 2401, 2830
ApeKI GCWGC 4 cut(s) 266, 269, 2014, 2195
ApoI RAATTY 3 cut(s) 1319, 2485, 2654
AseI ATTAAT 1 cut(s) 1779
AsiGI ACCGGT 2 cut(s) 1766, 2347
Asp700I GAANNNNTTC 2 cut(s) 504, 1887
AspLEI GCGC 5 cut(s) 1250, 2014, 2242, 2305, 2726
AspS9I GGNCC 4 cut(s) 699, 1844, 2329, 2831
AsuC2I CCSGG 1 cut(s) 2377
AsuNHI GCTAGC 1 cut(s) 1420
AvaI CYCGRG 1 cut(s) 2793
AvaII GGWCC 1 cut(s) 699
BalI TGGCCA 1 cut(s) 2403
BamHI GGATCC 2 cut(s) 1693, 2234
BanII GRGCYC 1 cut(s) 1868
BauI CACGAG 2 cut(s) 2018, 2801
BbsI GAAGAC 2 cut(s) 882, 1733
Bbv12I GWGCWC 1 cut(s) 1868
BbvI GCAGC 4 cut(s) 253, 256, 2001, 2207
BceAI ACGGC 6 cut(s) 917, 1323, 1706, 1800, 2265, 2538
BcgI CGANNNNNNTGC 2 cut(s) 1440, 1474
BciT130I CCWGG 2 cut(s) 1707, 2531
BciVI GTATCC 1 cut(s) 2278
BclI TGATCA 2 cut(s) 523, 2277
BcnI CCSGG 1 cut(s) 2377
BcoDI GTCTC 7 cut(s) 280, 478, 735, 743, 1049, 1298, 2233
BfaI CTAG 4 cut(s) 450, 677, 1421, 1635
BfmI CTRYAG 4 cut(s) 279, 758, 1836, 1898
BfoI RGCGCY 2 cut(s) 2306, 2727
BfuAI ACCTGC 2 cut(s) 751, 1891
BfuI GTATCC 1 cut(s) 2278
BisI GCNGC 5 cut(s) 267, 270, 1425, 2015, 2196
BlpI GCTNAGC 1 cut(s) 1606
BlsI GCNGC 5 cut(s) 268, 271, 1426, 2016, 2197
Bme1390I CCNGG 3 cut(s) 1707, 2377, 2531
Bme18I GGWCC 1 cut(s) 699
BmeRI GACNNNNNGTC 3 cut(s) 470, 987, 2348
BmeT110I CYCGRG 1 cut(s) 2793
BmgT120I GGNCC 4 cut(s) 699, 1844, 2329, 2831
BmiI GGNNCC 6 cut(s) 646, 1099, 1695, 2236, 2316, 2476
BmrFI CCNGG 3 cut(s) 1707, 2377, 2531
BmsI GCATC 7 cut(s) 47, 889, 1048, 1478, 1729, 2193, 2250
BmtI GCTAGC 1 cut(s) 1424
BoxI GACNNNNGTC 1 cut(s) 932
BpiI GAAGAC 2 cut(s) 882, 1733
Bpu1102I GCTNAGC 1 cut(s) 1606
BpuEI CTTGAG 2 cut(s) 2423, 2738
BpuMI CCSGG 1 cut(s) 2377
Bsa29I ATCGAT 2 cut(s) 21, 2571
BsaBI GATNNNNATC 1 cut(s) 2645
BsaI GGTCTC 2 cut(s) 280, 478
BsaJI CCNNGG 5 cut(s) 1706, 1923, 1938, 2040, 2792
BsaWI WCCGGW 2 cut(s) 1766, 2347
BsaXI ACNNNNNCTCC 6 cut(s) 489, 519, 1200, 1230, 2687, 2717
Bsc4I CCNNNNNNNGG 6 cut(s) 203, 539, 1439, 2327, 2351, 2560
Bse118I RCCGGY 2 cut(s) 1766, 2347
Bse1I ACTGG 5 cut(s) 417, 605, 890, 1990, 2476
Bse3DI GCAATG 1 cut(s) 1008
Bse8I GATNNNNATC 1 cut(s) 2645
BseBI CCWGG 2 cut(s) 1707, 2531
BseCI ATCGAT 2 cut(s) 21, 2571
BseDI CCNNGG 5 cut(s) 1706, 1923, 1938, 2040, 2792
BseGI GGATG 4 cut(s) 38, 1764, 2101, 2812
BseJI GATNNNNATC 1 cut(s) 2645
BseLI CCNNNNNNNGG 6 cut(s) 203, 539, 1439, 2327, 2351, 2560
BseMI GCAATG 1 cut(s) 1008
BseMII CTCAG 4 cut(s) 719, 2244, 2678, 2839
BseNI ACTGG 5 cut(s) 417, 605, 890, 1990, 2476
BseRI GAGGAG 3 cut(s) 99, 2781, 2813
BseXI GCAGC 4 cut(s) 253, 256, 2001, 2207
BseYI CCCAGC 2 cut(s) 1742, 2691
BsgI GTGCAG 1 cut(s) 2000
Bsh1236I CGCG 2 cut(s) 1250, 2240
Bsh1285I CGRYCG 1 cut(s) 2352
BshFI GGCC 6 cut(s) 174, 558, 1846, 2331, 2403, 2832
BshTI ACCGGT 2 cut(s) 1766, 2347
BshVI ATCGAT 2 cut(s) 21, 2571
BsiEI CGRYCG 1 cut(s) 2352
BsiHKAI GWGCWC 1 cut(s) 1868
BsiHKCI CYCGRG 1 cut(s) 2793
BsiSI CCGG 4 cut(s) 1767, 2348, 2376, 2455
BslFI GGGAC 4 cut(s) 723, 941, 1306, 2008
BslI CCNNNNNNNGG 6 cut(s) 203, 539, 1439, 2327, 2351, 2560
BsmAI GTCTC 7 cut(s) 280, 478, 735, 743, 1049, 1298, 2233
BsmBI CGTCTC 2 cut(s) 1298, 2233
BsmFI GGGAC 4 cut(s) 723, 941, 1306, 2008
BsmI GAATGC 5 cut(s) 312, 1332, 1454, 2080, 2534
BsnI GGCC 6 cut(s) 174, 558, 1846, 2331, 2403, 2832
Bso31I GGTCTC 2 cut(s) 280, 478
BsoBI CYCGRG 1 cut(s) 2793
Bsp1286I GDGCHC 1 cut(s) 1868
Bsp1407I TGTACA 1 cut(s) 2167
Bsp1720I GCTNAGC 1 cut(s) 1606
Bsp19I CCATGG 1 cut(s) 2040
BspACI CCGC 9 cut(s) 272, 815, 863, 1250, 1424, 1880, 2051, 2141, 2238
BspANI GGCC 6 cut(s) 174, 558, 1846, 2331, 2403, 2832
BspCNI CTCAG 4 cut(s) 720, 2243, 2679, 2838
BspDI ATCGAT 2 cut(s) 21, 2571
BspFNI CGCG 2 cut(s) 1250, 2240
BspHI TCATGA 2 cut(s) 55, 2607
BspLI GGNNCC 6 cut(s) 646, 1099, 1695, 2236, 2316, 2476
BspMAI CTGCAG 2 cut(s) 762, 1902
BspMI ACCTGC 2 cut(s) 751, 1891
BspOI GCTAGC 1 cut(s) 1424
BspPI GGATC 8 cut(s) 17, 1688, 1701, 1908, 2229, 2242, 2387, 2805
BspTNI GGTCTC 2 cut(s) 280, 478
BsrDI GCAATG 1 cut(s) 1008
BsrFI RCCGGY 2 cut(s) 1766, 2347
BsrGI TGTACA 1 cut(s) 2167
BsrI ACTGG 5 cut(s) 417, 605, 890, 1990, 2476
BssAI RCCGGY 2 cut(s) 1766, 2347
BssECI CCNNGG 5 cut(s) 1706, 1923, 1938, 2040, 2792
BssNAI GTATAC 1 cut(s) 1665
BssSI CACGAG 2 cut(s) 2018, 2801
BssT1I CCWWGG 3 cut(s) 1923, 1938, 2040
Bst1107I GTATAC 1 cut(s) 1665
Bst2BI CACGAG 2 cut(s) 2018, 2801
Bst2UI CCWGG 2 cut(s) 1707, 2531
BstAPI GCANNNNNTGC 2 cut(s) 44, 397
BstAUI TGTACA 1 cut(s) 2167
BstC8I GCNNGC 3 cut(s) 1422, 2224, 2307
BstDEI CTNAG 7 cut(s) 728, 1483, 1606, 1964, 2230, 2687, 2825
BstDSI CCRYGG 1 cut(s) 2040
BstEII GGTNACC 1 cut(s) 1762
BstENI CCTNNNNNAGG 1 cut(s) 201
BstF5I GGATG 4 cut(s) 38, 1764, 2101, 2812
BstFNI CGCG 2 cut(s) 1250, 2240
BstH2I RGCGCY 2 cut(s) 2306, 2727
BstHHI GCGC 5 cut(s) 1250, 2014, 2242, 2305, 2726
BstMAI GTCTC 7 cut(s) 280, 478, 735, 743, 1049, 1298, 2233
BstMCI CGRYCG 1 cut(s) 2352
BstMWI GCNNNNNNNGC 6 cut(s) 44, 397, 591, 1586, 2464, 2592
BstNI CCWGG 2 cut(s) 1707, 2531
BstNSI RCATGY 4 cut(s) 1053, 1584, 2226, 2590
BstPAI GACNNNNGTC 1 cut(s) 932
BstPI GGTNACC 1 cut(s) 1762
BstSCI CCNGG 3 cut(s) 1705, 2375, 2529
BstSFI CTRYAG 4 cut(s) 279, 758, 1836, 1898
BstUI CGCG 2 cut(s) 1250, 2240
BstV1I GCAGC 4 cut(s) 253, 256, 2001, 2207
BstV2I GAAGAC 2 cut(s) 882, 1733
BstX2I RGATCY 2 cut(s) 1693, 2234
BstXI CCANNNNNNTGG 1 cut(s) 2691
BstYI RGATCY 2 cut(s) 1693, 2234
BstZ17I GTATAC 1 cut(s) 1665
Bsu15I ATCGAT 2 cut(s) 21, 2571
BsuI GTATCC 1 cut(s) 2278
BsuRI GGCC 6 cut(s) 174, 558, 1846, 2331, 2403, 2832
BsuTUI ATCGAT 2 cut(s) 21, 2571
BtgI CCRYGG 1 cut(s) 2040
BtgZI GCGATG 1 cut(s) 2176
BtsCI GGATG 4 cut(s) 38, 1764, 2101, 2812
BtsI GCAGTG 3 cut(s) 395, 755, 984
BtsIMutI CAGTG 9 cut(s) 395, 612, 755, 984, 1363, 1509, 2587, 2684, 2833
BveI ACCTGC 2 cut(s) 751, 1891
Cac8I GCNNGC 3 cut(s) 1422, 2224, 2307
CaiI CAGNNNCTG 2 cut(s) 397, 1985
CciI TCATGA 2 cut(s) 55, 2607
CfoI GCGC 5 cut(s) 1250, 2014, 2242, 2305, 2726
Cfr10I RCCGGY 2 cut(s) 1766, 2347
Cfr13I GGNCC 4 cut(s) 699, 1844, 2329, 2831
ClaI ATCGAT 2 cut(s) 21, 2571
CseI GACGC 5 cut(s) 1691, 2012, 2215, 2489, 2701
Csp6I GTAC 2 cut(s) 1208, 2168
CspAI ACCGGT 2 cut(s) 1766, 2347
CspCI CAANNNNNGTGG 2 cut(s) 2428, 2463
CviQI GTAC 2 cut(s) 1208, 2168
DdeI CTNAG 7 cut(s) 728, 1483, 1606, 1964, 2230, 2687, 2825
DraI TTTAAA 2 cut(s) 406, 1348
DriI GACNNNNNGTC 3 cut(s) 470, 987, 2348
EaeI YGGCCR 1 cut(s) 2401
Eam1105I GACNNNNNGTC 3 cut(s) 470, 987, 2348
EciI GGCGGA 1 cut(s) 1895
Ecl136II GAGCTC 1 cut(s) 1866
Eco130I CCWWGG 3 cut(s) 1923, 1938, 2040
Eco24I GRGCYC 1 cut(s) 1868
Eco31I GGTCTC 2 cut(s) 280, 478
Eco32I GATATC 1 cut(s) 1564
Eco47I GGWCC 1 cut(s) 699
Eco53kI GAGCTC 1 cut(s) 1866
Eco57I CTGAAG 3 cut(s) 48, 321, 1221
Eco88I CYCGRG 1 cut(s) 2793
Eco91I GGTNACC 1 cut(s) 1762
EcoICRI GAGCTC 1 cut(s) 1866
EcoNI CCTNNNNNAGG 1 cut(s) 201
EcoO109I RGGNCCY 1 cut(s) 2329
EcoO65I GGTNACC 1 cut(s) 1762
EcoRI GAATTC 1 cut(s) 2654
EcoRII CCWGG 2 cut(s) 1705, 2529
EcoRV GATATC 1 cut(s) 1564
EcoT14I CCWWGG 3 cut(s) 1923, 1938, 2040
EcoT38I GRGCYC 1 cut(s) 1868
ErhI CCWWGG 3 cut(s) 1923, 1938, 2040
Esp3I CGTCTC 2 cut(s) 1298, 2233
FalI AAGNNNNNCTT 2 cut(s) 1922, 1954
FaqI GGGAC 4 cut(s) 723, 941, 1306, 2008
FauI CCCGC 1 cut(s) 2044
FauNDI CATATG 1 cut(s) 1116
FbaI TGATCA 2 cut(s) 523, 2277
FblI GTMKAC 4 cut(s) 463, 573, 1664, 2811
Fnu4HI GCNGC 5 cut(s) 267, 270, 1425, 2015, 2196
FokI GGATG 4 cut(s) 25, 1771, 2108, 2819
FriOI GRGCYC 1 cut(s) 1868
Fsp4HI GCNGC 5 cut(s) 267, 270, 1425, 2015, 2196
FspBI CTAG 4 cut(s) 450, 677, 1421, 1635
GlaI GCGC 5 cut(s) 1249, 2013, 2241, 2304, 2725
GluI GCNGC 5 cut(s) 267, 270, 1425, 2015, 2196
GsaI CCCAGC 2 cut(s) 1746, 2695
HaeII RGCGCY 2 cut(s) 2306, 2727
HaeIII GGCC 6 cut(s) 174, 558, 1846, 2331, 2403, 2832
HapII CCGG 4 cut(s) 1767, 2348, 2376, 2455
HgaI GACGC 5 cut(s) 1691, 2012, 2215, 2489, 2701
HhaI GCGC 5 cut(s) 1250, 2014, 2242, 2305, 2726
Hin6I GCGC 5 cut(s) 1248, 2012, 2240, 2303, 2724
HinP1I GCGC 5 cut(s) 1248, 2012, 2240, 2303, 2724
HincII GTYRAC 3 cut(s) 574, 2188, 2706
HindII GTYRAC 3 cut(s) 574, 2188, 2706
HindIII AAGCTT 3 cut(s) 583, 908, 1587
HinfI GANTC 5 cut(s) 578, 1294, 1628, 1686, 2451
HpaI GTTAAC 2 cut(s) 2188, 2706
HpaII CCGG 4 cut(s) 1767, 2348, 2376, 2455
Hpy99I CGWCG 2 cut(s) 578, 2483
HpyCH4IV ACGT 5 cut(s) 460, 983, 1667, 2121, 2361
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 397, 591, 1586, 2464, 2592
HpyF3I CTNAG 7 cut(s) 728, 1483, 1606, 1964, 2230, 2687, 2825
HpySE526I ACGT 5 cut(s) 460, 983, 1667, 2121, 2361
HspAI GCGC 5 cut(s) 1248, 2012, 2240, 2303, 2724
Ksp22I TGATCA 2 cut(s) 523, 2277
KspAI GTTAAC 2 cut(s) 2188, 2706
LmnI GCTCC 4 cut(s) 1466, 2011, 2314, 2794
Lsp1109I GCAGC 4 cut(s) 253, 256, 2001, 2207
LweI GCATC 7 cut(s) 47, 889, 1048, 1478, 1729, 2193, 2250
MaeI CTAG 4 cut(s) 450, 677, 1421, 1635
MaeII ACGT 5 cut(s) 460, 983, 1667, 2121, 2361
MboII GAAGA 7 cut(s) 41, 436, 626, 882, 1280, 1738, 2578
MflI RGATCY 2 cut(s) 1693, 2234
MhlI GDGCHC 1 cut(s) 1868
MlsI TGGCCA 1 cut(s) 2403
MluNI TGGCCA 1 cut(s) 2403
MlyI GAGTC 1 cut(s) 1288
MmeI TCCRAC 8 cut(s) 268, 475, 999, 1322, 1663, 2200, 2332, 2502
Mox20I TGGCCA 1 cut(s) 2403
MroXI GAANNNNTTC 2 cut(s) 504, 1887
MscI TGGCCA 1 cut(s) 2403
MslI CAYNNNNRTG 4 cut(s) 1940, 2606, 2612, 2805
Msp20I TGGCCA 1 cut(s) 2403
MspA1I CMGCKG 1 cut(s) 536
MspI CCGG 4 cut(s) 1767, 2348, 2376, 2455
MspR9I CCNGG 3 cut(s) 1707, 2377, 2531
Mva1269I GAATGC 5 cut(s) 312, 1332, 1454, 2080, 2534
MvaI CCWGG 2 cut(s) 1707, 2531
MvnI CGCG 2 cut(s) 1250, 2240
MwoI GCNNNNNNNGC 6 cut(s) 44, 397, 591, 1586, 2464, 2592
NciI CCSGG 1 cut(s) 2377
NcoI CCATGG 1 cut(s) 2040
NdeI CATATG 1 cut(s) 1116
NheI GCTAGC 1 cut(s) 1420
NlaIV GGNNCC 6 cut(s) 646, 1099, 1695, 2236, 2316, 2476
NmuCI GTSAC 7 cut(s) 367, 393, 445, 679, 979, 1762, 1769
NspI RCATGY 4 cut(s) 1053, 1584, 2226, 2590
OliI CACNNNNGTG 1 cut(s) 1940
PaeI GCATGC 1 cut(s) 2226
PagI TCATGA 2 cut(s) 55, 2607
PaqCI CACCTGC 1 cut(s) 751
PcsI WCGNNNNNNNCGW 4 cut(s) 2063, 2358, 2487, 2745
PctI GAATGC 5 cut(s) 312, 1332, 1454, 2080, 2534
PdmI GAANNNNTTC 2 cut(s) 504, 1887
PfeI GAWTC 4 cut(s) 578, 1628, 1686, 2451
PflMI CCANNNNNTGG 1 cut(s) 539
PinAI ACCGGT 2 cut(s) 1766, 2347
PkrI GCNGC 5 cut(s) 268, 271, 1426, 2016, 2197
PleI GAGTC 1 cut(s) 1288
PpsI GAGTC 1 cut(s) 1288
PshAI GACNNNNGTC 1 cut(s) 932
PshBI ATTAAT 1 cut(s) 1779
PsiI TTATAA 2 cut(s) 941, 1368
Psp124BI GAGCTC 1 cut(s) 1868
Psp1406I AACGTT 1 cut(s) 2121
Psp6I CCWGG 2 cut(s) 1705, 2529
PspEI GGTNACC 1 cut(s) 1762
PspFI CCCAGC 2 cut(s) 1742, 2691
PspGI CCWGG 2 cut(s) 1705, 2529
PspN4I GGNNCC 6 cut(s) 646, 1099, 1695, 2236, 2316, 2476
PspPI GGNCC 4 cut(s) 699, 1844, 2329, 2831
PstI CTGCAG 2 cut(s) 762, 1902
PstNI CAGNNNCTG 2 cut(s) 397, 1985
PsuI RGATCY 2 cut(s) 1693, 2234
PvuII CAGCTG 1 cut(s) 536
RsaI GTAC 2 cut(s) 1209, 2169
RsaNI GTAC 2 cut(s) 1208, 2168
RseI CAYNNNNRTG 4 cut(s) 1940, 2606, 2612, 2805
SacI GAGCTC 1 cut(s) 1868
SalI GTCGAC 1 cut(s) 572
SatI GCNGC 5 cut(s) 267, 270, 1425, 2015, 2196
Sau96I GGNCC 4 cut(s) 699, 1844, 2329, 2831
SchI GAGTC 1 cut(s) 1288
ScrFI CCNGG 3 cut(s) 1707, 2377, 2531
SduI GDGCHC 1 cut(s) 1868
SfaNI GCATC 7 cut(s) 47, 889, 1048, 1478, 1729, 2193, 2250
SfcI CTRYAG 4 cut(s) 279, 758, 1836, 1898
SgrAI CRCCGGYG 1 cut(s) 1766
SinI GGWCC 1 cut(s) 699
SmiMI CAYNNNNRTG 4 cut(s) 1940, 2606, 2612, 2805
SmlI CTYRAG 2 cut(s) 2438, 2753
SmoI CTYRAG 2 cut(s) 2438, 2753
SphI GCATGC 1 cut(s) 2226
SsiI CCGC 9 cut(s) 272, 815, 863, 1250, 1424, 1880, 2051, 2141, 2238
SspI AATATT 1 cut(s) 1777
SspMI CTAG 4 cut(s) 450, 677, 1421, 1635
SstI GAGCTC 1 cut(s) 1868
StyD4I CCNGG 3 cut(s) 1705, 2375, 2529
StyI CCWWGG 3 cut(s) 1923, 1938, 2040
TaiI ACGT 5 cut(s) 463, 986, 1670, 2124, 2364
TaqI TCGA 6 cut(s) 21, 49, 573, 926, 1912, 2571
TaqII GACCGA 1 cut(s) 716
TatI WGTACW 2 cut(s) 1207, 2167
TauI GCSGC 1 cut(s) 1427
TfiI GAWTC 4 cut(s) 578, 1628, 1686, 2451
TscAI CASTG 9 cut(s) 402, 612, 762, 984, 1363, 1516, 2587, 2691, 2833
TseFI GTSAC 7 cut(s) 367, 393, 445, 679, 979, 1762, 1769
TseI GCWGC 4 cut(s) 266, 269, 2014, 2195
Tsp45I GTSAC 7 cut(s) 367, 393, 445, 679, 979, 1762, 1769
TspDTI ATGAA 8 cut(s) 497, 538, 893, 1019, 2351, 2359, 2596, 2749
TspGWI ACGGA 5 cut(s) 96, 1817, 2072, 2327, 2479
TspRI CASTG 9 cut(s) 402, 612, 762, 984, 1363, 1516, 2587, 2691, 2833
Van91I CCANNNNNTGG 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 699
VspI ATTAAT 1 cut(s) 1779
XagI CCTNNNNNAGG 1 cut(s) 201
XapI RAATTY 3 cut(s) 1319, 2485, 2654
XceI RCATGY 4 cut(s) 1053, 1584, 2226, 2590
XmiI GTMKAC 4 cut(s) 463, 573, 1664, 2811
XmnI GAANNNNTTC 2 cut(s) 504, 1887
XspI CTAG 4 cut(s) 450, 677, 1421, 1635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.