pycom07g03500

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0010072
Physical Location & Seq
Forward (+)
2879984 .. 2889974
9991 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3201 bp
ATGAAGGTGCCGTGCTGCTCCGTCTGCCAGACCCGCTACGACGAGGAGGAAAGAGTTCCGCTTTTGCTTCAGTGCGGCCATGGCTTCTGCAAGGACTGCTTGTCCAAAATGTTCTCTTCCTGTCCCGATACTACCCTCGTCTGCCCGCGCTGTCGCCACGTCTCCGTCGTCGGGAACTCCGTCCAGGCGCTGAGGAAGAACTTCGCCGTGCTCGCCCTGATTCACTCCAGCTCCAATGCTGTCTCGTCTGCTGCCGCCGCCAATTTGGATTGCGATTACACGGACGACGAGGATGGGGACGATGATGAGGATGAGGATGGGGACCGAAGGTGTGCTCGTGGGTCCCACACGTCGATCTCGGGCGGGTGCGGGCCGGTGATCGAGCTGGCGGTGCACCCTGACTTGAAGTTGGTGCGGCGGATAGGGGAGGGGAGGCACACTGGGGTTCAAATGTGGACTGCCGTCATCGGAGGTGGAGGAGGGAGGTGCCGCCACCGGGTTGCGGTGAAGAAAGTGGCGGTAGCGGAGGAGACGAGTATGGATTGGGTGATGGGGCAGCTGGATAATTTGCGGCGGGCATCGATGTGGTGTAGGAATGTGTGCACATTTCATGGGGCTATTAAGAGTGAAGGCACCTTGTGTCTTGTTATGGATAAGTGTTATGGATCGGTTCAGTCCGAGATGCAGCGCAATGAGGGCAGGCTTACTTTGGAGCAAATTCTAAGATACGGGGCTGACATTGCACGAGGAGTGGCTGAACTTCATGCAGCAGGTGTTGTTTGTATGAATCTAAAACCGTCCAATCTTCTTTTGGATGAAAGTGGTCACGCGGTAGTTTCTGATTATGGAGTTGCTGCAATTCTGAAGAAGCCTTCCTGCCGGAAAGCTCGATTGGAGTGTGACACCTCAAGGATCCATTCTTGTATGGAGTGTACGATGCTCAGCCCCCACTATGCAGCTCCAGAGGCATGGGAACCAGTGAAGAAGTTACTGAATCCATTTTGGGAGGATGCTATCGGTATTTCTGCAGAGTCAGATGCATGGAGTTTTGGTTGTACATTGGTAGAAATGTGCACTGGTTCCATTCCATGGGCTGGTTTAAGCACTGAGGAAATTTATCGGGCTGTAATCAAGACTCGGAAACTACCTCCACAATATGCAAGTGTAGTAGGTGTTGGAATACCTAGAGAATTGTGGAAAATGATTGGCGAGTGCCTGCAGTTCAAGGCATCAAAAAGACCATCTTTTAATTCAATGCTAGCCACTTTTCTTCGCCATTTGCAGGAGATACCACGCAGCCCTCCTGCAAGTCCTGATAATGTTTTAGCTAAATGCTCTGGATCAAATGTGACGGAACCATCTCCTGTATCTGAATCAGAAGTTCTTCAGGGTAACCCCACTCTTCTGCATAGACTTGTGTCTGAAGGGGATGTGCGCAGTGTTAGAGATCTTCTTCAAAAGGCTTCAGCAGGGAGTGATAACAGCACAATATTGTCTCTATTAGAAGCTCAAAATATTGATGGCCAAACTGCTCTCCATTTGGCCTGTAGACGCGGCAGTGCAGTACTTGTTAATGCTATTTTGGAGTATCAAGAGGCGAATGTGGATGTCTTGGACAAAGATGGGGATCCTCCACTAGTTTTTGCATTAGTAGCTGGATCCCCAGAATGCGTTCATGCTCTCATTAACAGAGGTGCTAATGTGAGATCTAGGTTGCGAGAAGGCTTTGGTCCCTCGGTTGCTCATGTCTGTGCCTATCATGGCCAACCTGATTGTATGCGTGAGTTACTAATGGCTGGAGCGGATCCTAATGCGGTGGATGAGGAAGGTGAATCTGTACTGCATAGAGCTGTTGCAAAGAAATATACAGATTGTGCCCTTGTTGTTCTGGAAAATGGGGGCTCTAGGTCAATGTGCGTTTTGAATTCAGAAAAGTTTACACCATTGCACTTGTGTGTGGCAACGTGGAATGTTGCTGTTGTTAGAAGGTGGGTGGAAGTTGCAACACCAGAAGAGATTGCTGATGCAATTGATATACCAAGTCCAGTTGGCACTGCATTGTGTATGGCAGCTTCTCTAAAGAAAGATCATGAAATTGAAGGAAGAGAAATGGTGCAAATTTTGCTAGCTTCTGGAGCAGATCCAACTGCTCAAGATTTGCAGCATGGACGGACAGCTTTGCATACAGCTTCAATGGCCAATGAAGTTGAGTTGGTCAAGATTATACTTGATGCTGGAGTTGATGTGAACATTCGGAATGTACAAAATACAATTCCTCTTCACGTAGCACTGGCCAGAGGAGCAAAGTCATGTGTTGGACTGCTTCTCTCTGCTGGAGCAAATTATAATTTGCAGGATGATGAAGGCGACAATGCTTTCCATATAGCAGCAGATGCAGCAAAAATGATACGTGAGAATCTCGAATGGCTTATTGTCATGCTTAGAAATCCTGATGCTGCTGTTGAAGCTAGAAATCACAGTGGCAAGACCTTACGTGACTTTTTGGAGGCCCTTCCTCGGGAATGGATATCCGAAGATCTTATGGAGGCACTTGTAAATCGGGGAGTGCATTTGTCTCCTACAACATTTGATGTGGGAGACTGGGTCAAATTTAAAAGAAGTATAACAACTCCTACGTATGGATGGCAAGGTGCAAAACATAGAAGTGTTGGCTTTGTGCAAGGTGCCCCAGATAAGGACCATTTGATAGTATCATTTTGTTCTGGAGAGGCTCGTGTTTTGGCGAATGAAGTTGTAAAAGTTATTCCCTTGGACAGGGGACAGCATGTTCAGCTGAAACCAGATGTCAAAGAGCCCAGATTTGGGTGGCGTGGCCAGTCACGTGATAGCATTGGTACTGTATTATGTGTTGATGATGATGGTATCCTACGTGTTGGCTTTCCCGGAGCCTCCAGAGGGTGGAAAGCTGATCCAGCGGAGATGGAGAGAGTTGAAGAATTCAAGGTTGGGGATTGGGTTCGAATTCGCCCCACTCTTACAACTGCAAAGCACGGATTAGGATCTGTTACTCCTGGTAGCATTGGTATTGTGTATTGTATAAGACCAGATAGTAGTCTGTTACTGGAATTAAGCTATCTTCCAAGTCCATGGCATTGTGAACCAGAGGAGGTTGAGCCTGTAACCCCTTTCAGGATTGGTGACAGGGTATGCGTTAAGCGATCGATCTGTTGCAGAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5

Protein Analysis

1067

Amino Acids

116.16

Weight (kDa)

5.85

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 5 - 52 3.8e-06 Ring finger domain
zf-RING_UBOX PF13445 6 - 49 2.1e-06 RING-type zinc-finger
PK_Tyr_Ser-Thr PF07714 135 - 421 9.2e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 164 - 421 4e-30 Protein kinase domain
Ank_2 PF12796 469 - 570 5.9e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 561 - 617 8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 582 - 661 2.4e-09 Ankyrin repeats (3 copies)
Ank_4 PF13637 582 - 626 1.2e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 702 - 786 5.2e-14 Ankyrin repeats (3 copies)
Ank PF00023 722 - 753 6.9e-06 Ankyrin repeat
Ank_4 PF13637 727 - 776 4.4e-06 Ankyrin repeats (many copies)
SH3_15 PF18346 863 - 981 6.7e-19 Mind bomb SH3 repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2346
AarI CACCTGC 1 cut(s) 761
Acc16I TGCGCA 1 cut(s) 1436
Acc36I ACCTGC 1 cut(s) 761
AccB1I GGYRCC 4 cut(s) 7, 486, 632, 2684
AccB7I CCANNNNNTGG 3 cut(s) 1089, 1094, 2919
AccBSI CCGCTC 1 cut(s) 1802
AccI GTMKAC 1 cut(s) 1549
AccII CGCG 3 cut(s) 148, 830, 1554
AcoI YGGCCR 6 cut(s) 76, 1522, 1762, 2196, 2292, 2833
AcsI RAATTY 7 cut(s) 717, 1113, 1924, 2118, 2609, 2957, 2982
AcuI CTGAAG 5 cut(s) 53, 884, 1370, 1443, 1449
AcvI CACGTG 1 cut(s) 2843
AdeI CACNNNGTG 1 cut(s) 639
AfaI GTAC 6 cut(s) 934, 1057, 1566, 1839, 2262, 2857
AflIII ACRYGT 2 cut(s) 348, 2890
AhdI GACNNNNNGTC 1 cut(s) 100
AhlI ACTAGT 1 cut(s) 1636
AjiI CACGTC 2 cut(s) 160, 351
AjnI CCWGG 2 cut(s) 183, 3031
AjuI GAANNNNNNNTTGG 4 cut(s) 875, 907, 2949, 2981
AleI CACNNNNGTG 1 cut(s) 1953
AloI GAACNNNNNNTCC 2 cut(s) 2772, 2804
Alw21I GWGCWC 5 cut(s) 213, 337, 396, 605, 1076
Alw26I GTCTC 6 cut(s) 166, 247, 524, 1500, 2580, 2592
Alw44I GTGCAC 3 cut(s) 392, 601, 1072
AlwNI CAGNNNCTG 1 cut(s) 190
Ama87I CYCGRG 2 cut(s) 358, 2517
AoxI GGCC 9 cut(s) 76, 371, 1522, 1542, 1762, 2196, 2292, 2508, 2833
ApaLI GTGCAC 3 cut(s) 392, 601, 1072
ApoI RAATTY 7 cut(s) 717, 1113, 1924, 2118, 2609, 2957, 2982
Asp700I GAANNNNTTC 4 cut(s) 54, 200, 1383, 1671
AspLEI GCGC 4 cut(s) 150, 190, 690, 1437
AspS9I GGNCC 6 cut(s) 322, 342, 371, 1730, 2509, 2698
AsuC2I CCSGG 2 cut(s) 497, 2904
AsuHPI GGTGA 5 cut(s) 388, 517, 559, 1841, 3170
AsuII TTCGAA 1 cut(s) 2980
AsuNHI GCTAGC 2 cut(s) 1258, 2125
AvaI CYCGRG 2 cut(s) 358, 2517
AvaII GGWCC 4 cut(s) 322, 342, 1730, 2698
BaeGI GKGCMC 5 cut(s) 396, 605, 1076, 1879, 2689
BalI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2835
BamHI GGATCC 4 cut(s) 912, 1627, 1658, 1804
BanI GGYRCC 4 cut(s) 7, 486, 632, 2684
BanII GRGCYC 2 cut(s) 1904, 2817
BarI GAAGNNNNNNTAC 2 cut(s) 2056, 2088
BauI CACGAG 3 cut(s) 336, 744, 2733
BbrPI CACGTG 1 cut(s) 2843
Bbv12I GWGCWC 5 cut(s) 213, 337, 396, 605, 1076
BbvCI CCTCAGC 1 cut(s) 191
BceAI ACGGC 2 cut(s) 191, 446
BciT130I CCWGG 2 cut(s) 185, 3033
BciVI GTATCC 1 cut(s) 2894
BcnI CCSGG 2 cut(s) 497, 2904
BcoDI GTCTC 6 cut(s) 166, 247, 524, 1500, 2580, 2592
BcuI ACTAGT 1 cut(s) 1636
BfaI CTAG 8 cut(s) 1185, 1259, 1637, 1710, 1905, 2126, 2469, 3199
BfmI CTRYAG 3 cut(s) 1026, 1217, 1546
BfoI RGCGCY 1 cut(s) 191
BfuAI ACCTGC 1 cut(s) 761
BfuI GTATCC 1 cut(s) 2894
BglII AGATCT 3 cut(s) 1447, 1706, 2536
BlpI GCTNAGC 1 cut(s) 941
BmcAI AGTACT 1 cut(s) 1566
Bme1390I CCNGG 4 cut(s) 185, 497, 2904, 3033
Bme18I GGWCC 4 cut(s) 322, 342, 1730, 2698
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 2 cut(s) 358, 2517
BmgBI CACGTC 2 cut(s) 160, 351
BmgT120I GGNCC 6 cut(s) 322, 342, 371, 1730, 2509, 2698
BmrFI CCNGG 4 cut(s) 185, 497, 2904, 3033
BmrI ACTGGG 2 cut(s) 450, 2611
BmtI GCTAGC 2 cut(s) 1262, 2129
BmuI ACTGGG 2 cut(s) 450, 2611
BoxI GACNNNNGTC 2 cut(s) 461, 1417
BpmI CTGGAG 8 cut(s) 211, 945, 1818, 2154, 2256, 2355, 2745, 2896
Bpu10I CCTNAGC 1 cut(s) 191
Bpu1102I GCTNAGC 1 cut(s) 941
Bpu14I TTCGAA 1 cut(s) 2980
BpuEI CTTGAG 2 cut(s) 892, 2136
BpuMI CCSGG 2 cut(s) 497, 2904
Bsa29I ATCGAT 2 cut(s) 581, 3182
BsaAI YACGTR 6 cut(s) 2284, 2411, 2495, 2637, 2843, 2891
BsaBI GATNNNNATC 2 cut(s) 1626, 3019
BsaJI CCNNGG 6 cut(s) 79, 1088, 1734, 2516, 2769, 3107
BsaXI ACNNNNNCTCC 8 cut(s) 215, 245, 1036, 1066, 2229, 2259, 2589, 2619
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 8 cut(s) 445, 977, 1081, 2045, 2295, 2606, 2836, 3087
Bse3DI GCAATG 3 cut(s) 697, 738, 1943
Bse8I GATNNNNATC 2 cut(s) 1626, 3019
BseBI CCWGG 2 cut(s) 185, 3033
BseCI ATCGAT 2 cut(s) 581, 3182
BseDI CCNNGG 6 cut(s) 79, 1088, 1734, 2516, 2769, 3107
BseJI GATNNNNATC 2 cut(s) 1626, 3019
BseMI GCAATG 3 cut(s) 697, 738, 1943
BseMII CTCAG 3 cut(s) 182, 955, 1098
BseNI ACTGG 8 cut(s) 445, 977, 1081, 2045, 2295, 2606, 2836, 3087
BseRI GAGGAG 6 cut(s) 59, 492, 542, 762, 2313, 3140
BseSI GKGCMC 5 cut(s) 396, 605, 1076, 1879, 2689
BsgI GTGCAG 1 cut(s) 1581
Bsh1236I CGCG 3 cut(s) 148, 830, 1554
Bsh1285I CGRYCG 1 cut(s) 3182
BshFI GGCC 9 cut(s) 78, 373, 1524, 1544, 1764, 2198, 2294, 2510, 2835
BshNI GGYRCC 4 cut(s) 7, 486, 632, 2684
BshVI ATCGAT 2 cut(s) 581, 3182
BsiEI CGRYCG 1 cut(s) 3182
BsiHKAI GWGCWC 5 cut(s) 213, 337, 396, 605, 1076
BsiHKCI CYCGRG 2 cut(s) 358, 2517
BsiSI CCGG 4 cut(s) 374, 496, 880, 2904
BslFI GGGAC 6 cut(s) 108, 311, 328, 335, 1716, 2793
BsmAI GTCTC 6 cut(s) 166, 247, 524, 1500, 2580, 2592
BsmBI CGTCTC 2 cut(s) 166, 524
BsmFI GGGAC 6 cut(s) 108, 311, 328, 335, 1716, 2793
BsmI GAATGC 1 cut(s) 1673
BsnI GGCC 9 cut(s) 78, 373, 1524, 1544, 1764, 2198, 2294, 2510, 2835
BsoBI CYCGRG 2 cut(s) 358, 2517
Bsp119I TTCGAA 1 cut(s) 2980
Bsp1286I GDGCHC 9 cut(s) 213, 337, 396, 605, 1076, 1879, 1904, 2689, 2817
Bsp1407I TGTACA 2 cut(s) 1055, 2260
Bsp1720I GCTNAGC 1 cut(s) 941
Bsp19I CCATGG 3 cut(s) 79, 1088, 3107
BspANI GGCC 9 cut(s) 78, 373, 1524, 1544, 1764, 2198, 2294, 2510, 2835
BspCNI CTCAG 3 cut(s) 183, 954, 1099
BspDI ATCGAT 2 cut(s) 581, 3182
BspFNI CGCG 3 cut(s) 148, 830, 1554
BspHI TCATGA 1 cut(s) 2089
BspMAI CTGCAG 2 cut(s) 1030, 1221
BspMI ACCTGC 1 cut(s) 761
BspOI GCTAGC 2 cut(s) 1262, 2129
BspT104I TTCGAA 1 cut(s) 2980
BspT107I GGYRCC 4 cut(s) 7, 486, 632, 2684
BsrBI CCGCTC 1 cut(s) 1802
BsrDI GCAATG 3 cut(s) 697, 738, 1943
BsrFI RCCGGY 1 cut(s) 373
BsrGI TGTACA 2 cut(s) 1055, 2260
BsrI ACTGG 8 cut(s) 445, 977, 1081, 2045, 2295, 2606, 2836, 3087
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 6 cut(s) 79, 1088, 1734, 2516, 2769, 3107
BssSI CACGAG 3 cut(s) 336, 744, 2733
BssT1I CCWWGG 4 cut(s) 79, 1088, 2769, 3107
Bst2BI CACGAG 3 cut(s) 336, 744, 2733
Bst2UI CCWGG 2 cut(s) 185, 3033
Bst4CI ACNGT 3 cut(s) 798, 2480, 2860
Bst6I CTCTTC 5 cut(s) 121, 1407, 2007, 2098, 2283
BstAPI GCANNNNNTGC 3 cut(s) 96, 2122, 2393
BstAUI TGTACA 2 cut(s) 1055, 2260
BstBAI YACGTR 6 cut(s) 2284, 2411, 2495, 2637, 2843, 2891
BstBI TTCGAA 1 cut(s) 2980
BstC8I GCNNGC 9 cut(s) 146, 213, 371, 387, 576, 701, 1217, 1260, 2127
BstDEI CTNAG 5 cut(s) 191, 722, 941, 1107, 2441
BstDSI CCRYGG 3 cut(s) 79, 1088, 3107
BstEII GGTNACC 1 cut(s) 1391
BstENI CCTNNNNNAGG 1 cut(s) 2773
BstFNI CGCG 3 cut(s) 148, 830, 1554
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 4 cut(s) 150, 190, 690, 1437
BstMAI GTCTC 6 cut(s) 166, 247, 524, 1500, 2580, 2592
BstMCI CGRYCG 1 cut(s) 3182
BstNI CCWGG 2 cut(s) 185, 3033
BstNSI RCATGY 1 cut(s) 2789
BstPAI GACNNNNGTC 2 cut(s) 461, 1417
BstPI GGTNACC 1 cut(s) 1391
BstSCI CCNGG 4 cut(s) 183, 495, 2902, 3031
BstSFI CTRYAG 3 cut(s) 1026, 1217, 1546
BstSLI GKGCMC 5 cut(s) 396, 605, 1076, 1879, 2689
BstSNI TACGTA 1 cut(s) 2637
BstUI CGCG 3 cut(s) 148, 830, 1554
BstX2I RGATCY 9 cut(s) 912, 1447, 1627, 1658, 1706, 1804, 2140, 2536, 3020
BstXI CCANNNNNNTGG 2 cut(s) 969, 3108
BstYI RGATCY 9 cut(s) 912, 1447, 1627, 1658, 1706, 1804, 2140, 2536, 3020
Bsu15I ATCGAT 2 cut(s) 581, 3182
BsuI GTATCC 1 cut(s) 2894
BsuRI GGCC 9 cut(s) 78, 373, 1524, 1544, 1764, 2198, 2294, 2510, 2835
BsuTUI ATCGAT 2 cut(s) 581, 3182
BtgI CCRYGG 3 cut(s) 79, 1088, 3107
BtrI CACGTC 2 cut(s) 160, 351
BtsI GCAGTG 3 cut(s) 1444, 1564, 2052
BveI ACCTGC 1 cut(s) 761
Cac8I GCNNGC 9 cut(s) 146, 213, 371, 387, 576, 701, 1217, 1260, 2127
CaiI CAGNNNCTG 1 cut(s) 190
CciI TCATGA 1 cut(s) 2089
CfoI GCGC 4 cut(s) 150, 190, 690, 1437
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 6 cut(s) 322, 342, 371, 1730, 2509, 2698
ClaI ATCGAT 2 cut(s) 581, 3182
CseI GACGC 1 cut(s) 1560
Csp6I GTAC 6 cut(s) 933, 1056, 1565, 1838, 2261, 2856
CviQI GTAC 6 cut(s) 933, 1056, 1565, 1838, 2261, 2856
DdeI CTNAG 5 cut(s) 191, 722, 941, 1107, 2441
DraI TTTAAA 1 cut(s) 2614
DraIII CACNNNGTG 1 cut(s) 639
DriI GACNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 6 cut(s) 76, 1522, 1762, 2196, 2292, 2833
Eam1104I CTCTTC 5 cut(s) 121, 1407, 2007, 2098, 2283
Eam1105I GACNNNNNGTC 1 cut(s) 100
EarI CTCTTC 5 cut(s) 121, 1407, 2007, 2098, 2283
EciI GGCGGA 1 cut(s) 433
Eco105I TACGTA 1 cut(s) 2637
Eco130I CCWWGG 4 cut(s) 79, 1088, 2769, 3107
Eco24I GRGCYC 2 cut(s) 1904, 2817
Eco32I GATATC 1 cut(s) 2529
Eco47I GGWCC 4 cut(s) 322, 342, 1730, 2698
Eco57I CTGAAG 5 cut(s) 53, 884, 1370, 1443, 1449
Eco72I CACGTG 1 cut(s) 2843
Eco88I CYCGRG 2 cut(s) 358, 2517
Eco91I GGTNACC 1 cut(s) 1391
EcoNI CCTNNNNNAGG 1 cut(s) 2773
EcoO109I RGGNCCY 2 cut(s) 342, 2509
EcoO65I GGTNACC 1 cut(s) 1391
EcoRI GAATTC 3 cut(s) 1924, 2957, 2982
EcoRII CCWGG 2 cut(s) 183, 3031
EcoRV GATATC 1 cut(s) 2529
EcoT14I CCWWGG 4 cut(s) 79, 1088, 2769, 3107
EcoT22I ATGCAT 1 cut(s) 1042
EcoT38I GRGCYC 2 cut(s) 1904, 2817
ErhI CCWWGG 4 cut(s) 79, 1088, 2769, 3107
Esp3I CGTCTC 2 cut(s) 166, 524
FalI AAGNNNNNCTT 4 cut(s) 83, 115, 1228, 1260
FaqI GGGAC 6 cut(s) 108, 311, 328, 335, 1716, 2793
FauI CCCGC 5 cut(s) 41, 153, 356, 362, 567
FblI GTMKAC 1 cut(s) 1549
FriOI GRGCYC 2 cut(s) 1904, 2817
FspBI CTAG 8 cut(s) 1185, 1259, 1637, 1710, 1905, 2126, 2469, 3199
FspI TGCGCA 1 cut(s) 1436
GlaI GCGC 4 cut(s) 149, 189, 689, 1436
GsuI CTGGAG 8 cut(s) 211, 945, 1818, 2154, 2256, 2355, 2745, 2896
HaeII RGCGCY 1 cut(s) 191
HaeIII GGCC 9 cut(s) 78, 373, 1524, 1544, 1764, 2198, 2294, 2510, 2835
HapII CCGG 4 cut(s) 374, 496, 880, 2904
HgaI GACGC 1 cut(s) 1560
HhaI GCGC 4 cut(s) 150, 190, 690, 1437
Hin6I GCGC 4 cut(s) 148, 188, 688, 1435
HinP1I GCGC 4 cut(s) 148, 188, 688, 1435
HinfI GANTC 8 cut(s) 220, 787, 994, 1031, 1135, 1373, 1832, 2416
HpaII CCGG 4 cut(s) 374, 496, 880, 2904
HphI GGTGA 5 cut(s) 388, 517, 559, 1841, 3170
Hpy166II GTNNAC 9 cut(s) 394, 456, 603, 933, 1074, 1550, 1938, 2248, 3119
Hpy8I GTNNAC 9 cut(s) 394, 456, 603, 933, 1074, 1550, 1938, 2248, 3119
Hpy99I CGWCG 5 cut(s) 44, 170, 173, 290, 355
HpyCH4III ACNGT 3 cut(s) 798, 2480, 2860
HpyCH4IV ACGT 9 cut(s) 159, 350, 1964, 2283, 2410, 2494, 2636, 2842, 2890
HpyF3I CTNAG 5 cut(s) 191, 722, 941, 1107, 2441
HpySE526I ACGT 9 cut(s) 159, 350, 1964, 2283, 2410, 2494, 2636, 2842, 2890
HspAI GCGC 4 cut(s) 148, 188, 688, 1435
KflI GGGWCCC 1 cut(s) 342
LmnI GCTCC 9 cut(s) 23, 236, 712, 964, 1799, 2135, 2300, 2336, 2906
MaeI CTAG 8 cut(s) 1185, 1259, 1637, 1710, 1905, 2126, 2469, 3199
MaeII ACGT 9 cut(s) 159, 350, 1964, 2283, 2410, 2494, 2636, 2842, 2890
MbiI CCGCTC 1 cut(s) 1802
MfeI CAATTG 1 cut(s) 2028
MflI RGATCY 9 cut(s) 912, 1447, 1627, 1658, 1706, 1804, 2140, 2536, 3020
MhlI GDGCHC 9 cut(s) 213, 337, 396, 605, 1076, 1879, 1904, 2689, 2817
MlsI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2835
MluNI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2835
MlyI GAGTC 2 cut(s) 1040, 1129
MmeI TCCRAC 3 cut(s) 1156, 2168, 2296
Mox20I TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2835
Mph1103I ATGCAT 1 cut(s) 1042
MroXI GAANNNNTTC 4 cut(s) 54, 200, 1383, 1671
MscI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2835
MseI TTAA 8 cut(s) 621, 1100, 1248, 1572, 1686, 2613, 3089, 3174
MslI CAYNNNNRTG 4 cut(s) 583, 1749, 1953, 2664
Msp20I TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 2835
MspA1I CMGCKG 3 cut(s) 559, 2794, 2936
MspI CCGG 4 cut(s) 374, 496, 880, 2904
MspR9I CCNGG 4 cut(s) 185, 497, 2904, 3033
MunI CAATTG 1 cut(s) 2028
Mva1269I GAATGC 1 cut(s) 1673
MvaI CCWGG 2 cut(s) 185, 3033
MvnI CGCG 3 cut(s) 148, 830, 1554
NciI CCSGG 2 cut(s) 497, 2904
NcoI CCATGG 3 cut(s) 79, 1088, 3107
NheI GCTAGC 2 cut(s) 1258, 2125
NmuCI GTSAC 6 cut(s) 824, 899, 1348, 2495, 2838, 3158
NsbI TGCGCA 1 cut(s) 1436
NsiI ATGCAT 1 cut(s) 1042
NspI RCATGY 1 cut(s) 2789
NspV TTCGAA 1 cut(s) 2980
OliI CACNNNNGTG 1 cut(s) 1953
PagI TCATGA 1 cut(s) 2089
PaqCI CACCTGC 1 cut(s) 761
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 177
PctI GAATGC 1 cut(s) 1673
PdmI GAANNNNTTC 4 cut(s) 54, 200, 1383, 1671
PfeI GAWTC 6 cut(s) 220, 787, 994, 1373, 1832, 2416
PflFI GACNNNGTC 1 cut(s) 2603
PflMI CCANNNNNTGG 3 cut(s) 1089, 1094, 2919
PfoI TCCNGGA 1 cut(s) 2902
Ple19I CGATCG 1 cut(s) 3182
PleI GAGTC 2 cut(s) 1039, 1129
PmaCI CACGTG 1 cut(s) 2843
PmlI CACGTG 1 cut(s) 2843
PpsI GAGTC 2 cut(s) 1039, 1129
Ppu21I YACGTR 6 cut(s) 2284, 2411, 2495, 2637, 2843, 2891
PpuMI RGGWCCY 1 cut(s) 342
PshAI GACNNNNGTC 2 cut(s) 461, 1417
PsiI TTATAA 1 cut(s) 2346
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 2 cut(s) 183, 3031
PspCI CACGTG 1 cut(s) 2843
PspEI GGTNACC 1 cut(s) 1391
PspGI CCWGG 2 cut(s) 183, 3031
PspPI GGNCC 6 cut(s) 322, 342, 371, 1730, 2509, 2698
PspPPI RGGWCCY 1 cut(s) 342
PstI CTGCAG 2 cut(s) 1030, 1221
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 9 cut(s) 912, 1447, 1627, 1658, 1706, 1804, 2140, 2536, 3020
PsyI GACNNNGTC 1 cut(s) 2603
PvuI CGATCG 1 cut(s) 3182
PvuII CAGCTG 2 cut(s) 559, 2794
RsaI GTAC 6 cut(s) 934, 1057, 1566, 1839, 2262, 2857
RsaNI GTAC 6 cut(s) 933, 1056, 1565, 1838, 2261, 2856
RseI CAYNNNNRTG 4 cut(s) 583, 1749, 1953, 2664
SaqAI TTAA 8 cut(s) 621, 1100, 1248, 1572, 1686, 2613, 3089, 3174
Sau96I GGNCC 6 cut(s) 322, 342, 371, 1730, 2509, 2698
ScaI AGTACT 1 cut(s) 1566
SchI GAGTC 2 cut(s) 1040, 1129
ScrFI CCNGG 4 cut(s) 185, 497, 2904, 3033
SduI GDGCHC 9 cut(s) 213, 337, 396, 605, 1076, 1879, 1904, 2689, 2817
SfcI CTRYAG 3 cut(s) 1026, 1217, 1546
SfuI TTCGAA 1 cut(s) 2980
SinI GGWCC 4 cut(s) 322, 342, 1730, 2698
SmiMI CAYNNNNRTG 4 cut(s) 583, 1749, 1953, 2664
SmlI CTYRAG 2 cut(s) 907, 2151
SmoI CTYRAG 2 cut(s) 907, 2151
SnaBI TACGTA 1 cut(s) 2637
SpeI ACTAGT 1 cut(s) 1636
SspI AATATT 2 cut(s) 1491, 1516
SspMI CTAG 8 cut(s) 1185, 1259, 1637, 1710, 1905, 2126, 2469, 3199
StyD4I CCNGG 4 cut(s) 183, 495, 2902, 3031
StyI CCWWGG 4 cut(s) 79, 1088, 2769, 3107
TaaI ACNGT 3 cut(s) 798, 2480, 2860
TaiI ACGT 9 cut(s) 162, 353, 1967, 2286, 2413, 2497, 2639, 2845, 2893
TaqI TCGA 7 cut(s) 353, 381, 581, 889, 2421, 2980, 3182
TaqII GACCGA 1 cut(s) 339
TatI WGTACW 4 cut(s) 1055, 1564, 1837, 2260
TauI GCSGC 7 cut(s) 78, 257, 260, 418, 492, 574, 1557
TfiI GAWTC 6 cut(s) 220, 787, 994, 1373, 1832, 2416
Tru1I TTAA 8 cut(s) 621, 1100, 1248, 1572, 1686, 2613, 3089, 3174
Tru9I TTAA 8 cut(s) 621, 1100, 1248, 1572, 1686, 2613, 3089, 3174
TseFI GTSAC 6 cut(s) 824, 899, 1348, 2495, 2838, 3158
Tsp45I GTSAC 6 cut(s) 824, 899, 1348, 2495, 2838, 3158
TspGWI ACGGA 7 cut(s) 10, 154, 169, 296, 1367, 2185, 3027
Tth111I GACNNNGTC 1 cut(s) 2603
Van91I CCANNNNNTGG 3 cut(s) 1089, 1094, 2919
VneI GTGCAC 3 cut(s) 392, 601, 1072
VpaK11BI GGWCC 4 cut(s) 322, 342, 1730, 2698
XagI CCTNNNNNAGG 1 cut(s) 2773
XapI RAATTY 7 cut(s) 717, 1113, 1924, 2118, 2609, 2957, 2982
XceI RCATGY 1 cut(s) 2789
XcmI CCANNNNNNNNNTGG 1 cut(s) 808
XmiI GTMKAC 1 cut(s) 1549
XmnI GAANNNNTTC 4 cut(s) 54, 200, 1383, 1671
XspI CTAG 8 cut(s) 1185, 1259, 1637, 1710, 1905, 2126, 2469, 3199
ZrmI AGTACT 1 cut(s) 1566
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.