pycom07g06370

Plant nuclear matrix protein 1 (NMP1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0003865
Physical Location & Seq
Reverse (-)
5639184 .. 5644402
5219 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g06370.1

Sequence Viewer

Length: 987 bp
ATGGCAGCAAGACAAATGGAAGAGATACAGAGGAAGCTGGCGCTGCTGAATTATCCTAGAGCCAGTGCGGCTGCTCAGTCCCTCCTCTTCGCCGGCATGGAGCGCTACGCCCTTCTCGAGTGGCTCTTCTTCCGCTTGCTGGGGGACAAGTCGCCATTCTCTCAACAAAATCTTCAAGGGGACGCCAATGAAGAGACCGCTCGAATCCAGTATTTGGCAGAGATTGCAAAGTTTTTGGGTATTACGACTACTATTGACACAGAAGCCATCCAAGGGCGAGGAAGCTATGAAGATCGCACTGAAATGCTTCGTCTAATTGTAGATCTTGTGGAGGCGAGTATTTATGCTGATAATCAAGAATGGAGTACCGATGAGCAGGTAGCAAAGGACATACAACTAATTGATTCCATAGCTGAAAAACAAGCTCAAATATTCTCCGAGGAGTGCAAATTGTTCCCTGCAGATGTTCAGATTCAGTCCATATATCCATTGCCTGATGTTTCTGAATTGGAGAAAAAGCTTTCAGAACAATCAAAGATATTCTTAAGTCTTCAACAGAAGGTTGACGATTTGGCTTCAAAGCATGCTTACAACCCAGATGAGGATTATGCAGAGGTGGAATCAAGATTGCGTGCACATTTAGAGTCTTTTCTGGAAACTGCAAAATCATTCAATACGATTTACACTAAGGAAATACGTCCCTGGACACATATGATGGAGGTACCCCAGCTCCATGGATTTGGGCCGGCTGCCAATCGATTGCTGGAGGCATACAAGATGCTTTTGAAGTTCCTGGGGAACTTGAGGAATCTTAGAGACTCATACGCAGCTCTTGCTGTTGGATCGTCTGACTCAGTTGCTGGCGAGCCCTCATCTGTCACAAGAATAATCTCTGAGTGTGAATCTGCATTGACATTACTAAATCGCGACCTCGGGATTCTCTCAGCTTCCATTGCTCGCGAGCAAGGTGAGGAGGTGAGTTTATGA

Protein Analysis

329

Amino Acids

36.94

Weight (kDa)

4.74

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Plant_NMP1 PF06694 6 - 321 4.5e-189 Plant nuclear matrix protein 1 (NMP1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 367
Acc65I GGTACC 1 cut(s) 721
AccB1I GGYRCC 1 cut(s) 721
AccB7I CCANNNNNTGG 1 cut(s) 214
AccBSI CCGCTC 1 cut(s) 200
AccII CGCG 2 cut(s) 927, 960
AciI CCGC 3 cut(s) 68, 133, 198
AclWI GGATC 1 cut(s) 850
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 2 cut(s) 367, 723
AfeI AGCGCT 1 cut(s) 104
AfiI CCNNNNNNNGG 4 cut(s) 139, 214, 273, 601
AflII CTTAAG 1 cut(s) 544
AgsI TTSAA 5 cut(s) 176, 554, 579, 673, 787
AjnI CCWGG 2 cut(s) 701, 792
AluBI AGCT 8 cut(s) 37, 285, 413, 425, 520, 730, 830, 947
AluI AGCT 8 cut(s) 37, 285, 413, 425, 520, 730, 830, 947
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 2 cut(s) 188, 810
Alw44I GTGCAC 1 cut(s) 633
AlwI GGATC 1 cut(s) 850
AlwNI CAGNNNCTG 1 cut(s) 860
Ama87I CYCGRG 2 cut(s) 116, 932
Aor51HI AGCGCT 1 cut(s) 104
AoxI GGCC 1 cut(s) 743
ApaLI GTGCAC 1 cut(s) 633
ApeKI GCWGC 5 cut(s) 5, 43, 71, 749, 827
Asp700I GAANNNNTTC 1 cut(s) 306
Asp718I GGTACC 1 cut(s) 721
AspLEI GCGC 2 cut(s) 43, 105
AspS9I GGNCC 1 cut(s) 743
AsuHPI GGTGA 1 cut(s) 980
AvaI CYCGRG 2 cut(s) 116, 932
BaeGI GKGCMC 1 cut(s) 637
BanI GGYRCC 1 cut(s) 721
BanII GRGCYC 1 cut(s) 870
BbsI GAAGAC 1 cut(s) 542
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 5 cut(s) 17, 30, 58, 736, 839
BccI CCATC 2 cut(s) 275, 709
BciT130I CCWGG 2 cut(s) 703, 794
BcoDI GTCTC 2 cut(s) 188, 810
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 2 cut(s) 44, 106
BfrI CTTAAG 1 cut(s) 544
BfuAI ACCTGC 1 cut(s) 367
BglI GCCNNNNNGGC 1 cut(s) 68
BglII AGATCT 1 cut(s) 322
BisI GCNGC 6 cut(s) 6, 44, 69, 72, 750, 828
BlsI GCNGC 6 cut(s) 7, 45, 70, 73, 751, 829
Bme1390I CCNGG 2 cut(s) 703, 794
BmeT110I CYCGRG 2 cut(s) 116, 932
BmgT120I GGNCC 1 cut(s) 743
BmiI GGNNCC 1 cut(s) 723
BmrFI CCNGG 2 cut(s) 703, 794
BmsI GCATC 1 cut(s) 768
BpiI GAAGAC 1 cut(s) 542
BpmI CTGGAG 1 cut(s) 785
BpuEI CTTGAG 1 cut(s) 823
Bsa29I ATCGAT 1 cut(s) 757
BsaHI GRCGYC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 188
BsaJI CCNNGG 6 cut(s) 271, 438, 701, 733, 793, 931
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 4 cut(s) 139, 214, 273, 601
Bse118I RCCGGY 2 cut(s) 92, 745
Bse1I ACTGG 2 cut(s) 63, 208
Bse3DI GCAATG 2 cut(s) 488, 951
BseBI CCWGG 2 cut(s) 703, 794
BseCI ATCGAT 1 cut(s) 757
BseDI CCNNGG 6 cut(s) 271, 438, 701, 733, 793, 931
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 4 cut(s) 139, 214, 273, 601
BseMI GCAATG 2 cut(s) 488, 951
BseMII CTCAG 4 cut(s) 89, 867, 885, 957
BseNI ACTGG 2 cut(s) 63, 208
BseRI GAGGAG 3 cut(s) 74, 455, 986
BseSI GKGCMC 1 cut(s) 637
BseXI GCAGC 5 cut(s) 17, 30, 58, 736, 839
BseYI CCCAGC 2 cut(s) 139, 726
Bsh1236I CGCG 2 cut(s) 927, 960
BshFI GGCC 1 cut(s) 745
BshNI GGYRCC 1 cut(s) 721
BshVI ATCGAT 1 cut(s) 757
BsiHKAI GWGCWC 1 cut(s) 637
BsiHKCI CYCGRG 2 cut(s) 116, 932
BsiSI CCGG 2 cut(s) 93, 746
BslFI GGGAC 4 cut(s) 64, 158, 194, 684
BslI CCNNNNNNNGG 4 cut(s) 139, 214, 273, 601
BsmAI GTCTC 2 cut(s) 188, 810
BsmFI GGGAC 4 cut(s) 64, 158, 194, 684
BsnI GGCC 1 cut(s) 745
Bso31I GGTCTC 1 cut(s) 188
BsoBI CYCGRG 2 cut(s) 116, 932
Bsp1286I GDGCHC 2 cut(s) 637, 870
Bsp143I GATC 3 cut(s) 292, 322, 842
Bsp19I CCATGG 1 cut(s) 733
Bsp68I TCGCGA 2 cut(s) 927, 960
BspACI CCGC 3 cut(s) 68, 133, 198
BspANI GGCC 1 cut(s) 745
BspCNI CTCAG 4 cut(s) 88, 866, 886, 956
BspDI ATCGAT 1 cut(s) 757
BspFNI CGCG 2 cut(s) 927, 960
BspLI GGNNCC 1 cut(s) 723
BspMAI CTGCAG 1 cut(s) 463
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 1 cut(s) 850
BspQI GCTCTTC 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 721
BspTI CTTAAG 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 188
BsrBI CCGCTC 1 cut(s) 200
BsrDI GCAATG 2 cut(s) 488, 951
BsrFI RCCGGY 2 cut(s) 92, 745
BsrI ACTGG 2 cut(s) 63, 208
BssAI RCCGGY 2 cut(s) 92, 745
BssECI CCNNGG 6 cut(s) 271, 438, 701, 733, 793, 931
BssMI GATC 3 cut(s) 292, 322, 842
BssNI GRCGYC 1 cut(s) 183
BssT1I CCWWGG 2 cut(s) 271, 733
Bst2UI CCWGG 2 cut(s) 703, 794
Bst6I CTCTTC 4 cut(s) 15, 92, 131, 186
BstACI GRCGYC 1 cut(s) 183
BstAFI CTTAAG 1 cut(s) 544
BstAPI GCANNNNNTGC 2 cut(s) 224, 833
BstDEI CTNAG 6 cut(s) 75, 687, 812, 853, 894, 943
BstDSI CCRYGG 1 cut(s) 733
BstF5I GGATG 1 cut(s) 267
BstFNI CGCG 2 cut(s) 927, 960
BstH2I RGCGCY 2 cut(s) 44, 106
BstHHI GCGC 2 cut(s) 43, 105
BstKTI GATC 3 cut(s) 295, 325, 845
BstMAI GTCTC 2 cut(s) 188, 810
BstMBI GATC 3 cut(s) 292, 322, 842
BstMWI GCNNNNNNNGC 6 cut(s) 43, 68, 102, 224, 833, 953
BstNI CCWGG 2 cut(s) 703, 794
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 2 cut(s) 701, 792
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 637
BstUI CGCG 2 cut(s) 927, 960
BstV1I GCAGC 5 cut(s) 17, 30, 58, 736, 839
BstV2I GAAGAC 1 cut(s) 542
BstX2I RGATCY 1 cut(s) 322
BstXI CCANNNNNNTGG 2 cut(s) 734, 740
BstYI RGATCY 1 cut(s) 322
Bsu15I ATCGAT 1 cut(s) 757
BsuRI GGCC 1 cut(s) 745
BsuTUI ATCGAT 1 cut(s) 757
BtgI CCRYGG 1 cut(s) 733
BtsCI GGATG 1 cut(s) 267
BtsIMutI CAGTG 2 cut(s) 70, 297
BtuMI TCGCGA 2 cut(s) 927, 960
BveI ACCTGC 1 cut(s) 367
CaiI CAGNNNCTG 1 cut(s) 860
CfoI GCGC 2 cut(s) 43, 105
Cfr10I RCCGGY 2 cut(s) 92, 745
Cfr13I GGNCC 1 cut(s) 743
ClaI ATCGAT 1 cut(s) 757
CseI GACGC 1 cut(s) 191
Csp6I GTAC 2 cut(s) 366, 722
CviAII CATG 3 cut(s) 97, 584, 734
CviQI GTAC 2 cut(s) 366, 722
DdeI CTNAG 6 cut(s) 75, 687, 812, 853, 894, 943
DpnI GATC 3 cut(s) 294, 324, 844
DpnII GATC 3 cut(s) 292, 322, 842
Eam1104I CTCTTC 4 cut(s) 15, 92, 131, 186
EarI CTCTTC 4 cut(s) 15, 92, 131, 186
Eco130I CCWWGG 2 cut(s) 271, 733
Eco24I GRGCYC 1 cut(s) 870
Eco31I GGTCTC 1 cut(s) 188
Eco47III AGCGCT 1 cut(s) 104
Eco88I CYCGRG 2 cut(s) 116, 932
EcoRII CCWGG 2 cut(s) 701, 792
EcoT14I CCWWGG 2 cut(s) 271, 733
EcoT38I GRGCYC 1 cut(s) 870
ErhI CCWWGG 2 cut(s) 271, 733
FaeI CATG 3 cut(s) 100, 587, 737
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FaqI GGGAC 4 cut(s) 64, 158, 194, 684
FatI CATG 3 cut(s) 96, 583, 733
FauNDI CATATG 1 cut(s) 711
Fnu4HI GCNGC 6 cut(s) 6, 44, 69, 72, 750, 828
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 870
Fsp4HI GCNGC 6 cut(s) 6, 44, 69, 72, 750, 828
FspBI CTAG 1 cut(s) 57
GlaI GCGC 2 cut(s) 42, 104
GluI GCNGC 6 cut(s) 6, 44, 69, 72, 750, 828
GsaI CCCAGC 2 cut(s) 143, 730
GsuI CTGGAG 1 cut(s) 785
HaeII RGCGCY 2 cut(s) 44, 106
HaeIII GGCC 1 cut(s) 745
HapII CCGG 2 cut(s) 93, 746
HgaI GACGC 1 cut(s) 191
HhaI GCGC 2 cut(s) 43, 105
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 3 cut(s) 100, 587, 737
Hin6I GCGC 2 cut(s) 41, 103
HinP1I GCGC 2 cut(s) 41, 103
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 518
HpaII CCGG 2 cut(s) 93, 746
HphI GGTGA 1 cut(s) 980
Hpy166II GTNNAC 2 cut(s) 565, 635
Hpy188I TCNGA 6 cut(s) 439, 471, 505, 526, 850, 895
Hpy188III TCNNGA 7 cut(s) 116, 356, 624, 653, 926, 934, 959
Hpy8I GTNNAC 2 cut(s) 565, 635
HpyAV CCTTC 2 cut(s) 122, 553
HpyCH4IV ACGT 1 cut(s) 697
HpyCH4V TGCA 7 cut(s) 227, 447, 461, 611, 635, 662, 908
HpyF10VI GCNNNNNNNGC 6 cut(s) 43, 68, 102, 224, 833, 953
HpyF3I CTNAG 6 cut(s) 75, 687, 812, 853, 894, 943
HpySE526I ACGT 1 cut(s) 697
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 3 cut(s) 100, 587, 737
HspAI GCGC 2 cut(s) 41, 103
KpnI GGTACC 1 cut(s) 725
KroI GCCGGC 2 cut(s) 92, 745
KroNI GCCGGC 2 cut(s) 94, 747
Kzo9I GATC 3 cut(s) 292, 322, 842
LguI GCTCTTC 1 cut(s) 131
LmnI GCTCC 2 cut(s) 100, 735
Lsp1109I GCAGC 5 cut(s) 17, 30, 58, 736, 839
LweI GCATC 1 cut(s) 768
MaeI CTAG 1 cut(s) 57
MaeII ACGT 1 cut(s) 697
MaeIII GTNAC 1 cut(s) 877
MalI GATC 3 cut(s) 294, 324, 844
MbiI CCGCTC 1 cut(s) 200
MboI GATC 3 cut(s) 292, 322, 842
MboII GAAGA 8 cut(s) 32, 79, 118, 121, 164, 203, 302, 542
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 2 cut(s) 637, 870
MluCI AATT 5 cut(s) 49, 315, 399, 449, 506
MlyI GAGTC 3 cut(s) 653, 812, 845
MmeI TCCRAC 1 cut(s) 820
MroNI GCCGGC 2 cut(s) 92, 745
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 1 cut(s) 545
MslI CAYNNNNRTG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 544
MspI CCGG 2 cut(s) 93, 746
MspR9I CCNGG 2 cut(s) 703, 794
MvaI CCWGG 2 cut(s) 703, 794
MvnI CGCG 2 cut(s) 927, 960
MwoI GCNNNNNNNGC 6 cut(s) 43, 68, 102, 224, 833, 953
NaeI GCCGGC 2 cut(s) 94, 747
NcoI CCATGG 1 cut(s) 733
NdeI CATATG 1 cut(s) 711
NdeII GATC 3 cut(s) 292, 322, 842
NgoMIV GCCGGC 2 cut(s) 92, 745
NlaIII CATG 3 cut(s) 100, 587, 737
NlaIV GGNNCC 1 cut(s) 723
NmuCI GTSAC 1 cut(s) 877
NruI TCGCGA 2 cut(s) 927, 960
NspI RCATGY 1 cut(s) 587
PaeI GCATGC 1 cut(s) 587
PaeR7I CTCGAG 1 cut(s) 116
PciSI GCTCTTC 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PdiI GCCGGC 2 cut(s) 94, 747
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 7 cut(s) 204, 404, 472, 620, 808, 902, 937
PflMI CCANNNNNTGG 1 cut(s) 214
PkrI GCNGC 6 cut(s) 7, 45, 70, 73, 751, 829
PleI GAGTC 3 cut(s) 652, 812, 845
PpsI GAGTC 3 cut(s) 652, 812, 845
Psp6I CCWGG 2 cut(s) 701, 792
PspFI CCCAGC 2 cut(s) 139, 726
PspGI CCWGG 2 cut(s) 701, 792
PspN4I GGNNCC 1 cut(s) 723
PspPI GGNCC 1 cut(s) 743
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 860
PsuI RGATCY 1 cut(s) 322
RruI TCGCGA 2 cut(s) 927, 960
RsaI GTAC 2 cut(s) 367, 723
RsaNI GTAC 2 cut(s) 366, 722
RseI CAYNNNNRTG 1 cut(s) 302
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 545
SatI GCNGC 6 cut(s) 6, 44, 69, 72, 750, 828
Sau3AI GATC 3 cut(s) 292, 322, 842
Sau96I GGNCC 1 cut(s) 743
SchI GAGTC 3 cut(s) 653, 812, 845
ScrFI CCNGG 2 cut(s) 703, 794
SduI GDGCHC 2 cut(s) 637, 870
SfaNI GCATC 1 cut(s) 768
SfcI CTRYAG 1 cut(s) 459
Sfr274I CTCGAG 1 cut(s) 116
SlaI CTCGAG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 302
SmlI CTYRAG 3 cut(s) 116, 544, 802
SmoI CTYRAG 3 cut(s) 116, 544, 802
SphI GCATGC 1 cut(s) 587
Sse9I AATT 5 cut(s) 49, 315, 399, 449, 506
SsiI CCGC 3 cut(s) 68, 133, 198
SspI AATATT 1 cut(s) 432
SspMI CTAG 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 701, 792
StyI CCWWGG 2 cut(s) 271, 733
TaiI ACGT 1 cut(s) 700
TaqI TCGA 3 cut(s) 117, 202, 757
TasI AATT 5 cut(s) 49, 315, 399, 449, 506
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 7 cut(s) 204, 404, 472, 620, 808, 902, 937
Tru1I TTAA 1 cut(s) 545
Tru9I TTAA 1 cut(s) 545
TscAI CASTG 2 cut(s) 70, 304
TseFI GTSAC 1 cut(s) 877
TseI GCWGC 5 cut(s) 5, 43, 71, 749, 827
Tsp45I GTSAC 1 cut(s) 877
TspDTI ATGAA 2 cut(s) 204, 303
TspRI CASTG 2 cut(s) 70, 304
Van91I CCANNNNNTGG 1 cut(s) 214
Vha464I CTTAAG 1 cut(s) 544
VneI GTGCAC 1 cut(s) 633
XceI RCATGY 1 cut(s) 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 760
XhoI CTCGAG 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.