pycom07g09280

Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001252
Physical Location & Seq
Reverse (-)
8542767 .. 8543036
270 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g09280.1

Sequence Viewer

Length: 270 bp
ATGGTCACTGTGAATTCGACCCTCAGATTCTTGCAGCTCATCAAGCTTCCTCTTCATCCCCGACGAATAGTTATTTGCAAGCACATGCAAACTTCTATTTTCGTACTTAAGCTGATCGATCTCTTGCTTAAGTCTTTCCACCTCTGCCATCAACGATTCCACTTGGCGGGACCGAGCAAGCAGGCGTTGACCCATGTTGGACACAGAACTCGCACACTGAACACTAAGTGCGAGGGACTCTTGAACGGCCAACTCATCGGACCGTCCTGA

Protein Analysis

90

Amino Acids

10.13

Weight (kDa)

11.01

Isoelectric Point (pI)

22.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 167
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 1 cut(s) 13
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 1 cut(s) 105
AfiI CCNNNNNNNGG 1 cut(s) 166
AflII CTTAAG 2 cut(s) 107, 128
AgsI TTSAA 1 cut(s) 244
AluBI AGCT 3 cut(s) 37, 46, 112
AluI AGCT 3 cut(s) 37, 46, 112
AoxI GGCC 1 cut(s) 247
ApeKI GCWGC 1 cut(s) 34
ApoI RAATTY 1 cut(s) 13
AspS9I GGNCC 2 cut(s) 170, 260
AvaII GGWCC 2 cut(s) 170, 260
BbvI GCAGC 1 cut(s) 46
BccI CCATC 1 cut(s) 156
BceAI ACGGC 1 cut(s) 262
BfrI CTTAAG 2 cut(s) 107, 128
BisI GCNGC 1 cut(s) 35
BlsI GCNGC 1 cut(s) 36
Bme18I GGWCC 2 cut(s) 170, 260
BmgT120I GGNCC 2 cut(s) 170, 260
BmiI GGNNCC 1 cut(s) 171
Bsa29I ATCGAT 1 cut(s) 117
Bsc4I CCNNNNNNNGG 1 cut(s) 166
BseCI ATCGAT 1 cut(s) 117
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 37
BseXI GCAGC 1 cut(s) 46
BshFI GGCC 1 cut(s) 249
BshVI ATCGAT 1 cut(s) 117
BslFI GGGAC 2 cut(s) 183, 249
BslI CCNNNNNNNGG 1 cut(s) 166
BsmFI GGGAC 2 cut(s) 183, 249
BsnI GGCC 1 cut(s) 249
Bsp143I GATC 2 cut(s) 114, 118
BspACI CCGC 1 cut(s) 167
BspANI GGCC 1 cut(s) 249
BspCNI CTCAG 1 cut(s) 36
BspDI ATCGAT 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 171
BspTI CTTAAG 2 cut(s) 107, 128
BssMI GATC 2 cut(s) 114, 118
Bst4CI ACNGT 2 cut(s) 10, 264
Bst6I CTCTTC 1 cut(s) 57
BstAFI CTTAAG 2 cut(s) 107, 128
BstC8I GCNNGC 3 cut(s) 80, 179, 183
BstDEI CTNAG 2 cut(s) 23, 225
BstF5I GGATG 1 cut(s) 55
BstKTI GATC 2 cut(s) 117, 121
BstMBI GATC 2 cut(s) 114, 118
BstMWI GCNNNNNNNGC 1 cut(s) 43
BstNSI RCATGY 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 117
BsuRI GGCC 1 cut(s) 249
BsuTUI ATCGAT 1 cut(s) 117
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 2 cut(s) 6, 215
Cac8I GCNNGC 3 cut(s) 80, 179, 183
Cfr13I GGNCC 2 cut(s) 170, 260
ClaI ATCGAT 1 cut(s) 117
CpoI CGGWCCG 1 cut(s) 260
Csp6I GTAC 1 cut(s) 104
CspI CGGWCCG 1 cut(s) 260
CviAII CATG 2 cut(s) 85, 194
CviJI RGCY 4 cut(s) 37, 46, 112, 249
CviKI_1 RGCY 4 cut(s) 37, 46, 112, 249
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 2 cut(s) 23, 225
DpnI GATC 2 cut(s) 116, 120
DpnII GATC 2 cut(s) 114, 118
DraIII CACNNNGTG 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Eco47I GGWCC 2 cut(s) 170, 260
EcoRI GAATTC 1 cut(s) 13
FaeI CATG 2 cut(s) 88, 197
FaiI YATR 2 cut(s) 86, 195
FaqI GGGAC 2 cut(s) 183, 249
FatI CATG 2 cut(s) 84, 193
FauI CCCGC 1 cut(s) 160
Fnu4HI GCNGC 1 cut(s) 35
FokI GGATG 1 cut(s) 42
Fsp4HI GCNGC 1 cut(s) 35
GluI GCNGC 1 cut(s) 35
HaeIII GGCC 1 cut(s) 249
Hin1II CATG 2 cut(s) 88, 197
HincII GTYRAC 1 cut(s) 189
HindII GTYRAC 1 cut(s) 189
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 3 cut(s) 27, 156, 237
Hpy166II GTNNAC 1 cut(s) 189
Hpy188I TCNGA 2 cut(s) 26, 260
Hpy188III TCNNGA 2 cut(s) 241, 267
Hpy8I GTNNAC 1 cut(s) 189
Hpy99I CGWCG 1 cut(s) 66
HpyCH4III ACNGT 2 cut(s) 10, 264
HpyCH4V TGCA 3 cut(s) 34, 78, 88
HpyF10VI GCNNNNNNNGC 1 cut(s) 43
HpyF3I CTNAG 2 cut(s) 23, 225
Hsp92II CATG 2 cut(s) 88, 197
Kzo9I GATC 2 cut(s) 114, 118
LpnPI CCDG 1 cut(s) 167
Lsp1109I GCAGC 1 cut(s) 46
MaeIII GTNAC 1 cut(s) 4
MalI GATC 2 cut(s) 116, 120
MboI GATC 2 cut(s) 114, 118
MboII GAAGA 1 cut(s) 44
MluCI AATT 1 cut(s) 13
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 1 cut(s) 178
MnlI CCTC 4 cut(s) 32, 60, 152, 226
MseI TTAA 2 cut(s) 108, 129
MspCI CTTAAG 2 cut(s) 107, 128
MwoI GCNNNNNNNGC 1 cut(s) 43
NdeII GATC 2 cut(s) 114, 118
NlaIII CATG 2 cut(s) 88, 197
NlaIV GGNNCC 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 4
NspI RCATGY 1 cut(s) 88
PfeI GAWTC 2 cut(s) 27, 156
PkrI GCNGC 1 cut(s) 36
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
PspN4I GGNNCC 1 cut(s) 171
PspPI GGNCC 2 cut(s) 170, 260
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
Rsr2I CGGWCCG 1 cut(s) 260
RsrII CGGWCCG 1 cut(s) 260
SaqAI TTAA 2 cut(s) 108, 129
SatI GCNGC 1 cut(s) 35
Sau3AI GATC 2 cut(s) 114, 118
Sau96I GGNCC 2 cut(s) 170, 260
SchI GAGTC 1 cut(s) 231
SetI ASST 4 cut(s) 39, 48, 114, 144
SinI GGWCC 2 cut(s) 170, 260
SmlI CTYRAG 2 cut(s) 107, 128
SmoI CTYRAG 2 cut(s) 107, 128
Sse9I AATT 1 cut(s) 13
SsiI CCGC 1 cut(s) 167
TaaI ACNGT 2 cut(s) 10, 264
TaqI TCGA 2 cut(s) 17, 117
TaqII GACCGA 1 cut(s) 187
TasI AATT 1 cut(s) 13
TfiI GAWTC 2 cut(s) 27, 156
Tru1I TTAA 2 cut(s) 108, 129
Tru9I TTAA 2 cut(s) 108, 129
TscAI CASTG 2 cut(s) 13, 222
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 44
TspRI CASTG 2 cut(s) 13, 222
Vha464I CTTAAG 2 cut(s) 107, 128
VpaK11BI GGWCC 2 cut(s) 170, 260
XapI RAATTY 1 cut(s) 13
XceI RCATGY 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.