pycom12460g00010

Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012460
OG0001252
Physical Location & Seq
Reverse (-)
2494 .. 2763
270 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12460g00010.1

Sequence Viewer

Length: 270 bp
ATGGTCACTGTGAATTCGACCCTCAGATTCTTGCAGCTCATCAAGCTTCCTCTTCATCCCCAACGAATAGTTATTTGCAAGCACATGCAAACTTCTATTCTCGTACTTAAGCTGCTGGATCTCTTGCTTAAGTCTTTCCACCTCTGCCATCAACGATTCCACTTGGCGGGACCGAGCAAGCAGGCATTGGCCCATGTTGGACACAGAACTCGCACACTGAACACTAAGTGCGAGGGACTCTTGAACGGCCAACTCATCGGACCGTCCTGA

Protein Analysis

90

Amino Acids

10.04

Weight (kDa)

10.63

Isoelectric Point (pI)

20.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 167
AclWI GGATC 1 cut(s) 126
AcoI YGGCCR 1 cut(s) 247
AcsI RAATTY 1 cut(s) 13
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 1 cut(s) 105
AfiI CCNNNNNNNGG 1 cut(s) 166
AflII CTTAAG 2 cut(s) 107, 128
AgsI TTSAA 1 cut(s) 244
AluBI AGCT 3 cut(s) 37, 46, 112
AluI AGCT 3 cut(s) 37, 46, 112
AlwI GGATC 1 cut(s) 126
AoxI GGCC 2 cut(s) 189, 247
ApeKI GCWGC 2 cut(s) 34, 112
ApoI RAATTY 1 cut(s) 13
AspS9I GGNCC 3 cut(s) 170, 190, 260
AvaII GGWCC 2 cut(s) 170, 260
BbvI GCAGC 2 cut(s) 46, 99
BccI CCATC 1 cut(s) 156
BceAI ACGGC 1 cut(s) 262
BfrI CTTAAG 2 cut(s) 107, 128
BisI GCNGC 2 cut(s) 35, 113
BlsI GCNGC 2 cut(s) 36, 114
Bme18I GGWCC 2 cut(s) 170, 260
BmgT120I GGNCC 3 cut(s) 170, 190, 260
BmiI GGNNCC 1 cut(s) 171
Bsc4I CCNNNNNNNGG 1 cut(s) 166
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMII CTCAG 1 cut(s) 37
BseXI GCAGC 2 cut(s) 46, 99
BshFI GGCC 2 cut(s) 191, 249
BslFI GGGAC 2 cut(s) 183, 249
BslI CCNNNNNNNGG 1 cut(s) 166
BsmFI GGGAC 2 cut(s) 183, 249
BsnI GGCC 2 cut(s) 191, 249
Bsp143I GATC 1 cut(s) 118
BspACI CCGC 1 cut(s) 167
BspANI GGCC 2 cut(s) 191, 249
BspCNI CTCAG 1 cut(s) 36
BspLI GGNNCC 1 cut(s) 171
BspPI GGATC 1 cut(s) 126
BspTI CTTAAG 2 cut(s) 107, 128
BssMI GATC 1 cut(s) 118
Bst4CI ACNGT 2 cut(s) 10, 264
Bst6I CTCTTC 1 cut(s) 57
BstAFI CTTAAG 2 cut(s) 107, 128
BstC8I GCNNGC 3 cut(s) 80, 179, 183
BstDEI CTNAG 2 cut(s) 23, 225
BstF5I GGATG 1 cut(s) 55
BstKTI GATC 1 cut(s) 121
BstMBI GATC 1 cut(s) 118
BstMWI GCNNNNNNNGC 1 cut(s) 43
BstNSI RCATGY 1 cut(s) 88
BstV1I GCAGC 2 cut(s) 46, 99
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
BsuRI GGCC 2 cut(s) 191, 249
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 2 cut(s) 6, 215
Cac8I GCNNGC 3 cut(s) 80, 179, 183
Cfr13I GGNCC 3 cut(s) 170, 190, 260
CpoI CGGWCCG 1 cut(s) 260
Csp6I GTAC 1 cut(s) 104
CspI CGGWCCG 1 cut(s) 260
CviAII CATG 2 cut(s) 85, 194
CviJI RGCY 5 cut(s) 37, 46, 112, 191, 249
CviKI_1 RGCY 5 cut(s) 37, 46, 112, 191, 249
CviQI GTAC 1 cut(s) 104
DdeI CTNAG 2 cut(s) 23, 225
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
DraIII CACNNNGTG 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 247
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
Eco47I GGWCC 2 cut(s) 170, 260
EcoRI GAATTC 1 cut(s) 13
FaeI CATG 2 cut(s) 88, 197
FaiI YATR 2 cut(s) 86, 195
FaqI GGGAC 2 cut(s) 183, 249
FatI CATG 2 cut(s) 84, 193
FauI CCCGC 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 35, 113
FokI GGATG 1 cut(s) 42
Fsp4HI GCNGC 2 cut(s) 35, 113
GluI GCNGC 2 cut(s) 35, 113
HaeIII GGCC 2 cut(s) 191, 249
Hin1II CATG 2 cut(s) 88, 197
HindIII AAGCTT 1 cut(s) 44
HinfI GANTC 3 cut(s) 27, 156, 237
Hpy188I TCNGA 2 cut(s) 26, 260
Hpy188III TCNNGA 2 cut(s) 241, 267
HpyCH4III ACNGT 2 cut(s) 10, 264
HpyCH4V TGCA 3 cut(s) 34, 78, 88
HpyF10VI GCNNNNNNNGC 1 cut(s) 43
HpyF3I CTNAG 2 cut(s) 23, 225
Hsp92II CATG 2 cut(s) 88, 197
Kzo9I GATC 1 cut(s) 118
LpnPI CCDG 2 cut(s) 101, 167
Lsp1109I GCAGC 2 cut(s) 46, 99
MaeIII GTNAC 1 cut(s) 4
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 1 cut(s) 44
MflI RGATCY 1 cut(s) 118
MluCI AATT 1 cut(s) 13
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 1 cut(s) 178
MnlI CCTC 4 cut(s) 32, 60, 152, 226
MseI TTAA 2 cut(s) 108, 129
MspCI CTTAAG 2 cut(s) 107, 128
MwoI GCNNNNNNNGC 1 cut(s) 43
NdeII GATC 1 cut(s) 118
NlaIII CATG 2 cut(s) 88, 197
NlaIV GGNNCC 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 4
NspI RCATGY 1 cut(s) 88
PfeI GAWTC 2 cut(s) 27, 156
PkrI GCNGC 2 cut(s) 36, 114
PleI GAGTC 1 cut(s) 231
PpsI GAGTC 1 cut(s) 231
PspN4I GGNNCC 1 cut(s) 171
PspPI GGNCC 3 cut(s) 170, 190, 260
PsuI RGATCY 1 cut(s) 118
RsaI GTAC 1 cut(s) 105
RsaNI GTAC 1 cut(s) 104
Rsr2I CGGWCCG 1 cut(s) 260
RsrII CGGWCCG 1 cut(s) 260
SaqAI TTAA 2 cut(s) 108, 129
SatI GCNGC 2 cut(s) 35, 113
Sau3AI GATC 1 cut(s) 118
Sau96I GGNCC 3 cut(s) 170, 190, 260
SchI GAGTC 1 cut(s) 231
SetI ASST 4 cut(s) 39, 48, 114, 144
SinI GGWCC 2 cut(s) 170, 260
SmlI CTYRAG 2 cut(s) 107, 128
SmoI CTYRAG 2 cut(s) 107, 128
Sse9I AATT 1 cut(s) 13
SsiI CCGC 1 cut(s) 167
TaaI ACNGT 2 cut(s) 10, 264
TaqI TCGA 1 cut(s) 17
TaqII GACCGA 1 cut(s) 187
TasI AATT 1 cut(s) 13
TfiI GAWTC 2 cut(s) 27, 156
Tru1I TTAA 2 cut(s) 108, 129
Tru9I TTAA 2 cut(s) 108, 129
TscAI CASTG 2 cut(s) 13, 222
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 2 cut(s) 34, 112
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 44
TspRI CASTG 2 cut(s) 13, 222
Vha464I CTTAAG 2 cut(s) 107, 128
VpaK11BI GGWCC 2 cut(s) 170, 260
XapI RAATTY 1 cut(s) 13
XceI RCATGY 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.