Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0012306

Physical Location & Seq

Genomic Coordinates Forward (+)

8853352 .. 8859963

Gene Structure 6612 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGACGCGCTTCCCTCCACCCTCCACGCCACGCCTCCTTTACACCCTCTACAGATCTCGAACGGCGACGTCTCCTCCTTCCCCGTCCCCATCGGCAGCGTCTTCCCTCCTCATCGGCGCCTTCCACTTCCGCCAATTCTCCTCCGGCAACCTCGCGCGCGCCAAGGAGGACAAGGAGCCATGGTGGAAGGACTCCATGGACAAGCTTCGGAACATCGGAATCTCGGCCCACATCGACTCCGGCAAGACCACCCTCACCGAGAGAGTTCTGTTTTACACCGGGAAGATCCACGAGATTCACGAGGTTCGAGGCAGAGACGGCGTGGGTGCGAAAATGGATTCCATGGATTTGGAGAGGGAAAAGGGGATCACCATTCAGTCTGCTGCCACTTACTGTACTTGGAATGGCTATCAGATTAACATAATTGACACCCCAGGTCACGTTGATTTCACCATCGAGGTTGAGAGAGCTCTGCGTGTCCTTGATGGTGCCATTCTTGTCCTTTGTAGTGTTGGTGGAGTGCAAAGTCAGTCAATTACTGTTGATCGGCAAATGAAAAGATATGAGGTTCCAAGAATTGCATTTATAAACAAACTTGACCGAATGGGAGCAGATCCCTGGAAAGTTCTGAACCAGGCACGGGCTAAGCTCCGCCATCACAGTGCTGCCATGCAAGTTCCAATTGGGTTGGAAGAAGATTTTAAGGGTCTTATTGACCTTGTGCAGATGAAAGCTTACTATTTTCATGGTCCCAATGGTGAAAACATTATTACTGAAGAAGTTCCTGCTGATATGGAGGAGTTCGTCACAGAAAAGAGGCGCGAGCTAATTGAAGTTGTATCTGAAGTTGATGACAAACTAGCTGAAGCATTCCTTGATGATGAGCCCATATCACCTACTGATCTTGAGGAAGCTGTGCGGAGGGCTACTGTAGCAAGGAAGTTTATCCCTGTGTTCATGGGTAGTGCATTTAAAAACAAGGGTGTACAGCCACTTTTGAATGCTGTTCTTAATTATTTGCCTTGTCCGACTGAAGTCAGTAACTATGCTCTTGACCAGACTAATGATGAAGAGAAGGTTACATTAGGTGGAACTCCGGATGGGCCTCTTGTAGCATTGGCTTTCAAATTGGAGGAAGGGCGTTTTGGTCAGTTAACATATCTAAGAATCTATGAAGGTGTCATTCGGAAGGGTGATTTTATTTTTAACATTAATACCGGTAAAAGGATTAAGGTTCCTCGCTTGGTTCGGATGCATTCCAATGAGATGGAGGATATTCAAGAGGCACATGCTGGGCAAATCGTTGCTGTTTTTGGAGTGGACTGTGCATCAGGAGATACCTTTACTGATGGGTCGGTTAAATATACAATGACTTCTATGAGTGTCCCCGAGCCAGTGATGTCATTAGCTGTTCAACCAGTTTCAAAAGATTCTGGAGGACAATTCTCAAAGGCTTTGAATCGTTTTCAGAAAGAGGATCCAACATTTCGTGTTGGCTTGGATCCTGAGAGCGGACAGGTTGATGCCACTGTTGGAAAACCTCGTGTTAACTTCAGAGAGACTGTTACTCAGCGGGCTGAGTTTGATTATTTACATAAGAAGCAATCTGGAGGACAAGGTCAATATGGGCGGGTATGTGGGTATATTGAACCACTCCCTGCTGACTCACAGACCAAGTTTGAATTTGAAAACATTATTGTGGGACAAGCTATACCATCAAATTTTATTCCAGCTATTGAGAAGGGTTTCAAAGAAGCAGCCAATTCGGGTTCATTAATTGGACATCCAGTTGAGCATGTTCGTATTGTATTAACTGATGGCGCTTCCCATGCCGTGGACTCCAGTGAACTTGCCTTTAAATTAGCTGCAATTTATGCATTTAGAAAGTGCTACACGGCAGCAAGACCAGTGATATTGGAGCCCGTTATGCTGGTGGAATTGAAAGTACCTATAGAGTTTCAGGGTACTGTTGCTGGTGATCTTAACAAGAGAAAAGGTATCATTATTGGAAATGACCAGGAAGGAGATGACTCTGTTATTACTGCCCAAGTACCTCTGAACAACATGTTTGGGTATTCGACTTCTCTTCGGTCAATGACTCAGGGAAAAGGCGAATTCACAATGGAGTACAAAGAGCACTCGCCGGTTTCTAATGATGTGCAGGCTCAACTAGTCAAGAACTACAAGGGCACCACTGCTGCTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0003824 GO:0003924 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006412 GO:0006414 GO:0006518 GO:0006807 GO:0008135 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019538 GO:0030554 GO:0032543 GO:0032553 GO:0032555 GO:0032559 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0042221 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0070125 GO:0071704 GO:0097159 GO:0097367 GO:0140053 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

736

Amino Acids

81.39

Weight (kDa)

5.81

Isoelectric Point (pI)

40.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	68 - 341	7.7e-61	Elongation factor Tu GTP binding domain
EF-G_D2	PF22042	382 - 450	5.7e-10	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	383 - 449	2.2e-13	Elongation factor Tu domain 2
EFG_III	PF14492	462 - 507	4.2e-11	Elongation Factor G, domain III
EFG_IV	PF03764	514 - 634	7.3e-38	Elongation factor G, domain IV
EFG_C	PF00679	638 - 723	3.3e-26	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G45332 AT1G45332 AT2G45030
fragaria_vesca	FvH4_7g10500 FvH4_7g10500 FvH4_7g10500
malus_domestica	MD07G1111000.v1.1
prunus_persica	Prupe.2G134500_v2.0.a1
pyrus_communis	pycom07g09580
rosa_chinensis	RchiOBHm_Chr1g0348291
rosa_laevigata	RLG00000028669
rosa_multiflora	Rmu_sc0027364.1_g000002
rosa_roxburghii	Rroxscaffold_4G00307040
rosa_rugosa	Rorug01G0194100
rosa_samantha	Rh1AG211300 Rh1BG177800 Rh1CG195700 Rh1DG207300
rosa_wichuraiana	Rw1G017850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	587
AatII	GACGTC	1 cut(s)	71
AccB1I	GGYRCC	3 cut(s)	116, 488, 2189
AccB7I	CCANNNNNTGG	1 cut(s)	1834
AccBSI	CCGCTC	1 cut(s)	1512
AccII	CGCG	5 cut(s)	7, 155, 157, 159, 822
AccIII	TCCGGA	1 cut(s)	1096
AciI	CCGC	6 cut(s)	130, 652, 919, 1512, 1573, 1630
AclWI	GGATC	7 cut(s)	280, 374, 608, 1472, 1485, 1496, 1509
AcsI	RAATTY	3 cut(s)	1682, 1720, 2114
AcuI	CTGAAG	5 cut(s)	795, 864, 885, 1053, 1537
AcyI	GRCGYC	2 cut(s)	68, 117
AfaI	GTAC	6 cut(s)	397, 987, 1947, 1966, 2052, 2129
AfiI	CCNNNNNNNGG	4 cut(s)	640, 1224, 1511, 1834
AflIII	ACRYGT	1 cut(s)	2064
AgeI	ACCGGT	1 cut(s)	1217
AhlI	ACTAGT	1 cut(s)	2170
AjnI	CCWGG	4 cut(s)	433, 617, 633, 2016
AjuI	GAANNNNNNNTTGG	2 cut(s)	1128, 1160
Alw21I	GWGCWC	2 cut(s)	472, 2139
Alw26I	GTCTC	3 cut(s)	75, 309, 1553
AlwI	GGATC	7 cut(s)	280, 374, 608, 1472, 1485, 1496, 1509
Ama87I	CYCGRG	1 cut(s)	1388
Aor13HI	TCCGGA	1 cut(s)	1096
AoxI	GGCC	2 cut(s)	225, 1103
ApeKI	GCWGC	7 cut(s)	95, 383, 665, 1757, 1865, 1898, 2198
ApoI	RAATTY	3 cut(s)	1682, 1720, 2114
AseI	ATTAAT	2 cut(s)	1212, 1775
AsiGI	ACCGGT	1 cut(s)	1217
Asp700I	GAANNNNTTC	2 cut(s)	780, 1745
AspLEI	GCGC	7 cut(s)	9, 119, 157, 159, 161, 822, 1823
AspS9I	GGNCC	3 cut(s)	226, 749, 1103
AsuC2I	CCSGG	1 cut(s)	280
AsuHPI	GGTGA	7 cut(s)	247, 361, 442, 770, 885, 1205, 1988
AvaI	CYCGRG	1 cut(s)	1388
AvaII	GGWCC	1 cut(s)	749
BaeGI	GKGCMC	1 cut(s)	2192
BamHI	GGATCC	2 cut(s)	1477, 1501
BanI	GGYRCC	3 cut(s)	116, 488, 2189
BanII	GRGCYC	3 cut(s)	472, 888, 1923
BauI	CACGAG	3 cut(s)	290, 299, 1542
BbsI	GAAGAC	1 cut(s)	93
Bbv12I	GWGCWC	2 cut(s)	472, 2139
BbvI	GCAGC	7 cut(s)	107, 370, 652, 1769, 1852, 1910, 2185
BccI	CCATC	9 cut(s)	97, 461, 479, 663, 1094, 1261, 1343, 1723, 1811
BceAI	ACGGC	4 cut(s)	78, 334, 1817, 1911
BcgI	CGANNNNNNTGC	2 cut(s)	1746, 1780
BciT130I	CCWGG	4 cut(s)	435, 619, 635, 2018
BcnI	CCSGG	1 cut(s)	280
BcoDI	GTCTC	3 cut(s)	75, 309, 1553
BcuI	ACTAGT	1 cut(s)	2170
BfaI	CTAG	2 cut(s)	860, 2171
BfmI	CTRYAG	3 cut(s)	49, 930, 1950
BfoI	RGCGCY	2 cut(s)	120, 1824
BglII	AGATCT	1 cut(s)	53
BisI	GCNGC	7 cut(s)	96, 384, 666, 1758, 1866, 1899, 2199
BlpI	GCTNAGC	1 cut(s)	645
BlsI	GCNGC	7 cut(s)	97, 385, 667, 1759, 1867, 1900, 2200
Bme1390I	CCNGG	5 cut(s)	280, 435, 619, 635, 2018
Bme18I	GGWCC	1 cut(s)	749
BmeT110I	CYCGRG	1 cut(s)	1388
BmgT120I	GGNCC	3 cut(s)	226, 749, 1103
BmrFI	CCNGG	5 cut(s)	280, 435, 619, 635, 2018
BmsI	GCATC	3 cut(s)	1242, 1337, 1513
BoxI	GACNNNNGTC	1 cut(s)	1034
BpiI	GAAGAC	1 cut(s)	93
BpmI	CTGGAG	3 cut(s)	1455, 1629, 1825
Bpu1102I	GCTNAGC	1 cut(s)	645
BpuEI	CTTGAG	1 cut(s)	926
BpuMI	CCSGG	1 cut(s)	280
BsaHI	GRCGYC	2 cut(s)	68, 117
BsaJI	CCNNGG	7 cut(s)	162, 179, 195, 342, 433, 617, 1833
BsaWI	WCCGGW	2 cut(s)	1096, 1217
BsaXI	ACNNNNNCTCC	6 cut(s)	58, 88, 167, 197, 221, 251
Bsc4I	CCNNNNNNNGG	4 cut(s)	640, 1224, 1511, 1834
Bse118I	RCCGGY	2 cut(s)	1217, 2143
Bse1I	ACTGG	5 cut(s)	1394, 1418, 1787, 1842, 1907
BseAI	TCCGGA	1 cut(s)	1096
BseBI	CCWGG	4 cut(s)	435, 619, 635, 2018
BseDI	CCNNGG	7 cut(s)	162, 179, 195, 342, 433, 617, 1833
BseGI	GGATG	3 cut(s)	1105, 1257, 1783
BseLI	CCNNNNNNNGG	4 cut(s)	640, 1224, 1511, 1834
BseMII	CTCAG	4 cut(s)	1497, 1569, 1583, 2114
BseNI	ACTGG	5 cut(s)	1394, 1418, 1787, 1842, 1907
BsePI	GCGCGC	2 cut(s)	155, 157
BseRI	GAGGAG	4 cut(s)	63, 98, 130, 812
BseSI	GKGCMC	1 cut(s)	2192
BseXI	GCAGC	7 cut(s)	107, 370, 652, 1769, 1852, 1910, 2185
BseYI	CCCAGC	1 cut(s)	1292
BsgI	GTGCAG	2 cut(s)	743, 2180
Bsh1236I	CGCG	5 cut(s)	7, 155, 157, 159, 822
BshFI	GGCC	2 cut(s)	227, 1105
BshNI	GGYRCC	3 cut(s)	116, 488, 2189
BshTI	ACCGGT	1 cut(s)	1217
BsiHKAI	GWGCWC	2 cut(s)	472, 2139
BsiHKCI	CYCGRG	1 cut(s)	1388
BsiSI	CCGG	6 cut(s)	144, 240, 279, 1097, 1218, 2144
BslFI	GGGAC	4 cut(s)	70, 735, 1370, 1716
BslI	CCNNNNNNNGG	4 cut(s)	640, 1224, 1511, 1834
BsmAI	GTCTC	3 cut(s)	75, 309, 1553
BsmBI	CGTCTC	2 cut(s)	75, 309
BsmFI	GGGAC	4 cut(s)	70, 735, 1370, 1716
BsmI	GAATGC	3 cut(s)	869, 1006, 1255
BsnI	GGCC	2 cut(s)	227, 1105
BsoBI	CYCGRG	1 cut(s)	1388
Bsp1286I	GDGCHC	5 cut(s)	472, 888, 1923, 2139, 2192
Bsp13I	TCCGGA	1 cut(s)	1096
Bsp1407I	TGTACA	1 cut(s)	985
Bsp143I	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
Bsp1720I	GCTNAGC	1 cut(s)	645
Bsp19I	CCATGG	3 cut(s)	179, 195, 342
BspACI	CCGC	6 cut(s)	130, 652, 919, 1512, 1573, 1630
BspANI	GGCC	2 cut(s)	227, 1105
BspCNI	CTCAG	4 cut(s)	1498, 1570, 1582, 2113
BspEI	TCCGGA	1 cut(s)	1096
BspFNI	CGCG	5 cut(s)	7, 155, 157, 159, 822
BspPI	GGATC	7 cut(s)	280, 374, 608, 1472, 1485, 1496, 1509
BspT107I	GGYRCC	3 cut(s)	116, 488, 2189
BsrBI	CCGCTC	1 cut(s)	1512
BsrFI	RCCGGY	2 cut(s)	1217, 2143
BsrGI	TGTACA	1 cut(s)	985
BsrI	ACTGG	5 cut(s)	1394, 1418, 1787, 1842, 1907
BssAI	RCCGGY	2 cut(s)	1217, 2143
BssECI	CCNNGG	7 cut(s)	162, 179, 195, 342, 433, 617, 1833
BssHII	GCGCGC	2 cut(s)	155, 157
BssMI	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
BssNI	GRCGYC	2 cut(s)	68, 117
BssSI	CACGAG	3 cut(s)	290, 299, 1542
BssT1I	CCWWGG	4 cut(s)	162, 179, 195, 342
Bst2BI	CACGAG	3 cut(s)	290, 299, 1542
Bst2UI	CCWGG	4 cut(s)	435, 619, 635, 2018
Bst4CI	ACNGT	8 cut(s)	395, 541, 662, 931, 1325, 1531, 1564, 1969
Bst6I	CTCTTC	2 cut(s)	1065, 2091
BstACI	GRCGYC	2 cut(s)	68, 117
BstAPI	GCANNNNNTGC	1 cut(s)	1874
BstAUI	TGTACA	1 cut(s)	985
BstC8I	GCNNGC	5 cut(s)	157, 159, 824, 1575, 2163
BstDEI	CTNAG	6 cut(s)	645, 1163, 1506, 1569, 1578, 2100
BstDSI	CCRYGG	4 cut(s)	179, 195, 342, 1833
BstF5I	GGATG	3 cut(s)	1105, 1257, 1783
BstFNI	CGCG	5 cut(s)	7, 155, 157, 159, 822
BstH2I	RGCGCY	2 cut(s)	120, 1824
BstHHI	GCGC	7 cut(s)	9, 119, 157, 159, 161, 822, 1823
BstKTI	GATC	9 cut(s)	56, 288, 369, 547, 616, 904, 1480, 1504, 1981
BstMAI	GTCTC	3 cut(s)	75, 309, 1553
BstMBI	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
BstMWI	GCNNNNNNNGC	5 cut(s)	318, 932, 1829, 1874, 1927
BstNI	CCWGG	4 cut(s)	435, 619, 635, 2018
BstNSI	RCATGY	3 cut(s)	1292, 1799, 2068
BstPAI	GACNNNNGTC	1 cut(s)	1034
BstSCI	CCNGG	5 cut(s)	278, 433, 617, 633, 2016
BstSFI	CTRYAG	3 cut(s)	49, 930, 1950
BstSLI	GKGCMC	1 cut(s)	2192
BstUI	CGCG	5 cut(s)	7, 155, 157, 159, 822
BstV1I	GCAGC	7 cut(s)	107, 370, 652, 1769, 1852, 1910, 2185
BstV2I	GAAGAC	1 cut(s)	93
BstX2I	RGATCY	5 cut(s)	53, 285, 613, 1477, 1501
BstXI	CCANNNNNNTGG	2 cut(s)	349, 1267
BstYI	RGATCY	5 cut(s)	53, 285, 613, 1477, 1501
BsuRI	GGCC	2 cut(s)	227, 1105
BtgI	CCRYGG	4 cut(s)	179, 195, 342, 1833
BtsCI	GGATG	3 cut(s)	1105, 1257, 1783
BtsI	GCAGTG	1 cut(s)	2193
BtsIMutI	CAGTG	6 cut(s)	667, 1401, 1527, 1849, 1914, 2193
Cac8I	GCNNGC	5 cut(s)	157, 159, 824, 1575, 2163
CfoI	GCGC	7 cut(s)	9, 119, 157, 159, 161, 822, 1823
Cfr10I	RCCGGY	2 cut(s)	1217, 2143
Cfr13I	GGNCC	3 cut(s)	226, 749, 1103
CseI	GACGC	2 cut(s)	13, 87
Csp6I	GTAC	6 cut(s)	396, 986, 1946, 1965, 2051, 2128
CspAI	ACCGGT	1 cut(s)	1217
CviQI	GTAC	6 cut(s)	396, 986, 1946, 1965, 2051, 2128
DdeI	CTNAG	6 cut(s)	645, 1163, 1506, 1569, 1578, 2100
DinI	GGCGCC	1 cut(s)	118
DpnI	GATC	9 cut(s)	55, 287, 368, 546, 615, 903, 1479, 1503, 1980
DpnII	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
DraI	TTTAAA	2 cut(s)	973, 1858
Eam1104I	CTCTTC	2 cut(s)	1065, 2091
EarI	CTCTTC	2 cut(s)	1065, 2091
EciI	GGCGGA	2 cut(s)	119, 641
Ecl136II	GAGCTC	1 cut(s)	470
Eco130I	CCWWGG	4 cut(s)	162, 179, 195, 342
Eco24I	GRGCYC	3 cut(s)	472, 888, 1923
Eco47I	GGWCC	1 cut(s)	749
Eco53kI	GAGCTC	1 cut(s)	470
Eco57I	CTGAAG	5 cut(s)	795, 864, 885, 1053, 1537
Eco88I	CYCGRG	1 cut(s)	1388
EcoICRI	GAGCTC	1 cut(s)	470
EcoRI	GAATTC	1 cut(s)	2114
EcoRII	CCWGG	4 cut(s)	433, 617, 633, 2016
EcoT14I	CCWWGG	4 cut(s)	162, 179, 195, 342
EcoT22I	ATGCAT	2 cut(s)	1257, 1879
EcoT38I	GRGCYC	3 cut(s)	472, 888, 1923
EgeI	GGCGCC	1 cut(s)	118
EheI	GGCGCC	1 cut(s)	118
ErhI	CCWWGG	4 cut(s)	162, 179, 195, 342
Esp3I	CGTCTC	2 cut(s)	75, 309
FalI	AAGNNNNNCTT	2 cut(s)	858, 890
FaqI	GGGAC	4 cut(s)	70, 735, 1370, 1716
FauI	CCCGC	2 cut(s)	1566, 1623
Fnu4HI	GCNGC	7 cut(s)	96, 384, 666, 1758, 1866, 1899, 2199
FokI	GGATG	3 cut(s)	1112, 1264, 1770
FriOI	GRGCYC	3 cut(s)	472, 888, 1923
Fsp4HI	GCNGC	7 cut(s)	96, 384, 666, 1758, 1866, 1899, 2199
FspBI	CTAG	2 cut(s)	860, 2171
GlaI	GCGC	7 cut(s)	8, 118, 156, 158, 160, 821, 1822
GluI	GCNGC	7 cut(s)	96, 384, 666, 1758, 1866, 1899, 2199
GsaI	CCCAGC	1 cut(s)	1296
GsuI	CTGGAG	3 cut(s)	1455, 1629, 1825
HaeII	RGCGCY	2 cut(s)	120, 1824
HaeIII	GGCC	2 cut(s)	227, 1105
HapII	CCGG	6 cut(s)	144, 240, 279, 1097, 1218, 2144
HgaI	GACGC	2 cut(s)	13, 87
HhaI	GCGC	7 cut(s)	9, 119, 157, 159, 161, 822, 1823
Hin1I	GRCGYC	2 cut(s)	68, 117
Hin6I	GCGC	7 cut(s)	7, 117, 155, 157, 159, 820, 1821
HinP1I	GCGC	7 cut(s)	7, 117, 155, 157, 159, 820, 1821
HincII	GTYRAC	2 cut(s)	1155, 1549
HindII	GTYRAC	2 cut(s)	1155, 1549
HindIII	AAGCTT	2 cut(s)	203, 732
HpaI	GTTAAC	2 cut(s)	1155, 1549
HpaII	CCGG	6 cut(s)	144, 240, 279, 1097, 1218, 2144
HphI	GGTGA	7 cut(s)	247, 361, 442, 770, 885, 1205, 1988
Hpy166II	GTNNAC	6 cut(s)	986, 1155, 1321, 1549, 1837, 1847
Hpy8I	GTNNAC	6 cut(s)	986, 1155, 1321, 1549, 1837, 1847
Hpy99I	CGWCG	1 cut(s)	70
HpyAV	CCTTC	9 cut(s)	87, 130, 181, 1069, 1130, 1169, 1183, 1735, 2015
HpyCH4III	ACNGT	8 cut(s)	395, 541, 662, 931, 1325, 1531, 1564, 1969
HpyCH4IV	ACGT	2 cut(s)	68, 441
HpyF10VI	GCNNNNNNNGC	5 cut(s)	318, 932, 1829, 1874, 1927
HpyF3I	CTNAG	6 cut(s)	645, 1163, 1506, 1569, 1578, 2100
HpySE526I	ACGT	2 cut(s)	68, 441
Hsp92I	GRCGYC	2 cut(s)	68, 117
HspAI	GCGC	7 cut(s)	7, 117, 155, 157, 159, 820, 1821
KasI	GGCGCC	1 cut(s)	116
Kpn2I	TCCGGA	1 cut(s)	1096
KspAI	GTTAAC	2 cut(s)	1155, 1549
Kzo9I	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
LmnI	GCTCC	4 cut(s)	175, 608, 654, 1918
Lsp1109I	GCAGC	7 cut(s)	107, 370, 652, 1769, 1852, 1910, 2185
LweI	GCATC	3 cut(s)	1242, 1337, 1513
MaeI	CTAG	2 cut(s)	860, 2171
MaeII	ACGT	2 cut(s)	68, 441
MaeIII	GTNAC	5 cut(s)	437, 805, 1040, 1078, 1564
MalI	GATC	9 cut(s)	55, 287, 368, 546, 615, 903, 1479, 1503, 1980
MauBI	CGCGCGCG	1 cut(s)	155
MbiI	CCGCTC	1 cut(s)	1512
MboI	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
MboII	GAAGA	7 cut(s)	93, 295, 704, 707, 788, 1082, 2078
MfeI	CAATTG	1 cut(s)	681
MflI	RGATCY	5 cut(s)	53, 285, 613, 1477, 1501
MhlI	GDGCHC	5 cut(s)	472, 888, 1923, 2139, 2192
Mly113I	GGCGCC	1 cut(s)	117
MlyI	GAGTC	6 cut(s)	185, 230, 1658, 1832, 2024, 2092
MmeI	TCCRAC	4 cut(s)	669, 1052, 1505, 1513
Mph1103I	ATGCAT	2 cut(s)	1257, 1879
MroI	TCCGGA	1 cut(s)	1096
MroXI	GAANNNNTTC	2 cut(s)	780, 1745
MslI	CAYNNNNRTG	3 cut(s)	660, 1260, 1697
MspA1I	CMGCKG	1 cut(s)	1573
MspI	CCGG	6 cut(s)	144, 240, 279, 1097, 1218, 2144
MspR9I	CCNGG	5 cut(s)	280, 435, 619, 635, 2018
MunI	CAATTG	1 cut(s)	681
Mva1269I	GAATGC	3 cut(s)	869, 1006, 1255
MvaI	CCWGG	4 cut(s)	435, 619, 635, 2018
MvnI	CGCG	5 cut(s)	7, 155, 157, 159, 822
MwoI	GCNNNNNNNGC	5 cut(s)	318, 932, 1829, 1874, 1927
NarI	GGCGCC	1 cut(s)	117
NciI	CCSGG	1 cut(s)	280
NcoI	CCATGG	3 cut(s)	179, 195, 342
NdeII	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
NmeAIII	GCCGAG	1 cut(s)	203
NmuCI	GTSAC	2 cut(s)	437, 805
NsiI	ATGCAT	2 cut(s)	1257, 1879
NspI	RCATGY	3 cut(s)	1292, 1799, 2068
PauI	GCGCGC	2 cut(s)	155, 157
PciI	ACATGT	1 cut(s)	2064
PcsI	WCGNNNNNNNCGW	1 cut(s)	297
PctI	GAATGC	3 cut(s)	869, 1006, 1255
PdmI	GAANNNNTTC	2 cut(s)	780, 1745
PfeI	GAWTC	6 cut(s)	219, 295, 338, 1167, 1430, 1459
PflFI	GACNNNGTC	1 cut(s)	1617
PflMI	CCANNNNNTGG	1 cut(s)	1834
PinAI	ACCGGT	1 cut(s)	1217
PkrI	GCNGC	7 cut(s)	97, 385, 667, 1759, 1867, 1900, 2200
PleI	GAGTC	6 cut(s)	185, 230, 1658, 1832, 2024, 2092
PluTI	GGCGCC	1 cut(s)	120
PpsI	GAGTC	6 cut(s)	185, 230, 1658, 1832, 2024, 2092
PscI	ACATGT	1 cut(s)	2064
PshAI	GACNNNNGTC	1 cut(s)	1034
PshBI	ATTAAT	2 cut(s)	1212, 1775
PsiI	TTATAA	1 cut(s)	587
Psp124BI	GAGCTC	1 cut(s)	472
Psp6I	CCWGG	4 cut(s)	433, 617, 633, 2016
PspFI	CCCAGC	1 cut(s)	1292
PspGI	CCWGG	4 cut(s)	433, 617, 633, 2016
PspPI	GGNCC	3 cut(s)	226, 749, 1103
PsuI	RGATCY	5 cut(s)	53, 285, 613, 1477, 1501
PsyI	GACNNNGTC	1 cut(s)	1617
PteI	GCGCGC	2 cut(s)	155, 157
RsaI	GTAC	6 cut(s)	397, 987, 1947, 1966, 2052, 2129
RsaNI	GTAC	6 cut(s)	396, 986, 1946, 1965, 2051, 2128
RseI	CAYNNNNRTG	3 cut(s)	660, 1260, 1697
SacI	GAGCTC	1 cut(s)	472
SatI	GCNGC	7 cut(s)	96, 384, 666, 1758, 1866, 1899, 2199
Sau3AI	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
Sau96I	GGNCC	3 cut(s)	226, 749, 1103
SchI	GAGTC	6 cut(s)	185, 230, 1658, 1832, 2024, 2092
ScrFI	CCNGG	5 cut(s)	280, 435, 619, 635, 2018
SduI	GDGCHC	5 cut(s)	472, 888, 1923, 2139, 2192
SfaNI	GCATC	3 cut(s)	1242, 1337, 1513
SfcI	CTRYAG	3 cut(s)	49, 930, 1950
SfoI	GGCGCC	1 cut(s)	118
SinI	GGWCC	1 cut(s)	749
SmiMI	CAYNNNNRTG	3 cut(s)	660, 1260, 1697
SmlI	CTYRAG	1 cut(s)	905
SmoI	CTYRAG	1 cut(s)	905
SpeI	ACTAGT	1 cut(s)	2170
SsiI	CCGC	6 cut(s)	130, 652, 919, 1512, 1573, 1630
SspDI	GGCGCC	1 cut(s)	116
SspMI	CTAG	2 cut(s)	860, 2171
SstI	GAGCTC	1 cut(s)	472
StyD4I	CCNGG	5 cut(s)	278, 433, 617, 633, 2016
StyI	CCWWGG	4 cut(s)	162, 179, 195, 342
TaaI	ACNGT	8 cut(s)	395, 541, 662, 931, 1325, 1531, 1564, 1969
TaiI	ACGT	2 cut(s)	71, 444
TaqI	TCGA	5 cut(s)	58, 234, 307, 456, 2078
TaqII	GACCGA	2 cut(s)	615, 2079
TatI	WGTACW	3 cut(s)	395, 985, 2127
TfiI	GAWTC	6 cut(s)	219, 295, 338, 1167, 1430, 1459
TscAI	CASTG	6 cut(s)	667, 1401, 1534, 1849, 1914, 2200
TseFI	GTSAC	2 cut(s)	437, 805
TseI	GCWGC	7 cut(s)	95, 383, 665, 1757, 1865, 1898, 2198
Tsp45I	GTSAC	2 cut(s)	437, 805
TspDTI	ATGAA	7 cut(s)	569, 734, 743, 946, 1083, 1188, 1761
TspRI	CASTG	6 cut(s)	667, 1401, 1534, 1849, 1914, 2200
Tth111I	GACNNNGTC	1 cut(s)	1617
Van91I	CCANNNNNTGG	1 cut(s)	1834
VpaK11BI	GGWCC	1 cut(s)	749
VspI	ATTAAT	2 cut(s)	1212, 1775
XapI	RAATTY	3 cut(s)	1682, 1720, 2114
XceI	RCATGY	3 cut(s)	1292, 1799, 2068
XmnI	GAANNNNTTC	2 cut(s)	780, 1745
XspI	CTAG	2 cut(s)	860, 2171
ZraI	GACGTC	1 cut(s)	69
Zsp2I	ATGCAT	2 cut(s)	1257, 1879

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.