pycom07g11640

Acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007995
Physical Location & Seq
Reverse (-)
12758835 .. 12764735
5901 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1668 bp
ATGGCAGACAACGATCTCACCTCCCCCTTCCTCTCTCCTCCACCTTCCGATCACCCCCACTTGATCCTCACCATCCAAGACGACACCGATACCGAATCCCACCACAACCACAACGGCATCCACACCCATCAGAGCACCAGCGCCGACCACCACCACCACTCTCGCAACCCATTTGCATTTCTCGGGTCCGATGGGTTGACCGTGCCCGTTTCCACCACCGCGGACCCGTTTCGGAACCACACCCTCGAGATTACGGGTATTTACGAGTGGTTAAAGATCGGAATTTGCCTGCCCATTGCGCTGGTTCGGCTGGTGCTGTTTGGGGCGTCTTTGCTGATTGGGTTTTTGGCGACGAAATTGGCTCTTCAGGGGTGGAAGGATAAGAAGAGCCCTATGCCGAGGTGGAGGTGCAGGATTATGTGGATCACTAGGGTCTGTACTCGATGCATTCTCTTCGCTTTTGGCTACCATTGGATAAGACGGAAAGGAAAACCTTCTCCCCGAGACATTGCTCCAATAGTTGTTTCTAACCATGTGTCTTTTATTGAACCTATCTTCTATTTCTATGAATTATTCCCTACAATTGTGGCATCCGAATCCCATGATTCCCTACCTTTTGTTGGGACGATCATCAGAGCAATGCAGGTAATATATGTTAACAGGTTTTCAGCATCATCAAGGAAGCATGCTGTCAGTGAAATCAAGAGAAAAGCTTCATGTGGTAGATTTCCTCGAGTTCTTTTATTTCCCGAGGGAACCACAACCAACGGGAGATATCTCATTTCATTTGAGCTTGGTGCATTCATCCCTGGTTTTGCCATCCAACCAGTAGTCGTACGATATCCCCATGTACACTTTGATCAATCCTGGGGGCACATTTCTTTGGCAAAGCTCATGTTTAGAATGTTCACACAGTTTCACAATTTCATGGAGGTAGAGTACCTTTCTGTTGTTTCACTCTTTGATAACAAGAAAGAAAGTGCTACCCGTTTTTCGGAGAGGACTAGTCATGCTATTGCAACTGCACTCAACGTTGTACAGACGTCTCATTCATATGGAGATTTAATGCTTCTCATGAAAGCATCTCAGTTAAAATTGAAACTGGAGCAACCTTCAGTGTATATGGTTGAAATGGCAAGTGTAAAGTCATTACTCCATATAAGCAGCATGGAGGCTGTGGACTTTCTAGATAAGTTTCTTTGTATGAATCCAGACCCAAGAGGTCATGTTCACTACCATGATTTCCTAAGGGTGCTAAGACTCAAGGCTTGTAGCTATTCCAAAGAGATATTTGCATTCATGGATGTTGAGAGAAATGGAGTAATAACGTTTAAGCAGTTCTTATTTGGATCAGCGCATGTCGTTAAGCGGCCATTGTTCCGCAGAGCCTGTGAATTAGCCTTTTCTGAATGTGTTTCTGGGGAGAATGACTACGTTTCAGAACAAAATTTTGGAGAGTCCATCGGACATGCAATCCCGGATTTGAATGAGGATGAGGTCCGTGGACTGTGCAATCTATTTGATTCTGATAATGATGGAAGAATCAGCAAGGAAGATTTTTTGACCTGCCTAAGAAAAAACCCACTACTGATTGCGGTTTTCTCGCCTTGTTTGCTTAACAAAGACATTTCAGAAGATGGAAATAGGTTGGTGGAGGAGATTGTGTAG

Protein Analysis

556

Amino Acids

63.12

Weight (kDa)

7.39

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransferase PF01553 167 - 279 2.4e-14 Acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1046
Acc36I ACCTGC 2 cut(s) 634, 1574
AccII CGCG 1 cut(s) 221
AciI CCGC 5 cut(s) 219, 221, 1369, 1381, 1595
AclI AACGTT 2 cut(s) 1032, 1328
AclWI GGATC 3 cut(s) 58, 431, 1357
AcoI YGGCCR 1 cut(s) 1370
AcsI RAATTY 2 cut(s) 282, 1447
AcuI CTGAAG 2 cut(s) 350, 1098
AcyI GRCGYC 2 cut(s) 326, 1043
AfaI GTAC 5 cut(s) 439, 837, 852, 941, 1038
AfiI CCNNNNNNNGG 2 cut(s) 620, 994
AgsI TTSAA 4 cut(s) 548, 1099, 1130, 1486
AhlI ACTAGT 1 cut(s) 1004
AjnI CCWGG 2 cut(s) 808, 866
AjuI GAANNNNNNNTTGG 4 cut(s) 508, 540, 1434, 1466
AluBI AGCT 4 cut(s) 713, 793, 892, 1275
AluI AGCT 4 cut(s) 713, 793, 892, 1275
Alw21I GWGCWC 1 cut(s) 137
Alw26I GTCTC 2 cut(s) 498, 1050
AlwI GGATC 3 cut(s) 58, 431, 1357
AlwNI CAGNNNCTG 1 cut(s) 1389
Ama87I CYCGRG 5 cut(s) 182, 245, 501, 732, 749
AoxI GGCC 1 cut(s) 1370
ApeKI GCWGC 1 cut(s) 1164
ApoI RAATTY 2 cut(s) 282, 1447
Asp700I GAANNNNTTC 2 cut(s) 493, 712
AspLEI GCGC 3 cut(s) 143, 301, 1357
AspS9I GGNCC 3 cut(s) 186, 223, 1498
AsuC2I CCSGG 1 cut(s) 1478
AsuHPI GGTGA 3 cut(s) 10, 44, 61
AvaI CYCGRG 5 cut(s) 182, 245, 501, 732, 749
AvaII GGWCC 3 cut(s) 186, 223, 1498
AxyI CCTNAGG 1 cut(s) 1247
BaeGI GKGCMC 2 cut(s) 207, 876
BanII GRGCYC 1 cut(s) 392
Bbv12I GWGCWC 1 cut(s) 137
BbvI GCAGC 1 cut(s) 1176
BccI CCATC 7 cut(s) 80, 135, 185, 827, 1469, 1529, 1631
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BciT130I CCWGG 2 cut(s) 810, 868
BclI TGATCA 1 cut(s) 859
BcnI CCSGG 1 cut(s) 1478
BcoDI GTCTC 2 cut(s) 498, 1050
BcuI ACTAGT 1 cut(s) 1004
BfaI CTAG 3 cut(s) 429, 1005, 1187
BfoI RGCGCY 1 cut(s) 144
BfuAI ACCTGC 2 cut(s) 634, 1574
BisI GCNGC 2 cut(s) 1165, 1370
BlsI GCNGC 2 cut(s) 1166, 1371
Bme1390I CCNGG 3 cut(s) 810, 868, 1478
Bme18I GGWCC 3 cut(s) 186, 223, 1498
BmeT110I CYCGRG 5 cut(s) 182, 245, 501, 732, 749
BmgT120I GGNCC 3 cut(s) 186, 223, 1498
BmiI GGNNCC 4 cut(s) 187, 225, 236, 757
BmrFI CCNGG 3 cut(s) 810, 868, 1478
BmsI GCATC 5 cut(s) 126, 434, 599, 680, 1091
BpmI CTGGAG 1 cut(s) 1124
BpuEI CTTGAG 1 cut(s) 1247
BpuMI CCSGG 1 cut(s) 1478
BsaHI GRCGYC 2 cut(s) 326, 1043
BsaJI CCNNGG 6 cut(s) 219, 398, 750, 808, 867, 1501
BsaXI ACNNNNNCTCC 3 cut(s) 923, 953, 1649
Bsc4I CCNNNNNNNGG 2 cut(s) 620, 994
Bse1I ACTGG 2 cut(s) 827, 1107
Bse21I CCTNAGG 1 cut(s) 1247
Bse3DI GCAATG 3 cut(s) 294, 507, 645
BseBI CCWGG 2 cut(s) 810, 868
BseDI CCNNGG 6 cut(s) 219, 398, 750, 808, 867, 1501
BseGI GGATG 7 cut(s) 72, 117, 590, 804, 819, 1309, 1498
BseLI CCNNNNNNNGG 2 cut(s) 620, 994
BseMI GCAATG 3 cut(s) 294, 507, 645
BseMII CTCAG 1 cut(s) 1100
BseNI ACTGG 2 cut(s) 827, 1107
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 2 cut(s) 207, 876
BseXI GCAGC 1 cut(s) 1176
BsgI GTGCAG 2 cut(s) 430, 1008
Bsh1236I CGCG 1 cut(s) 221
BshFI GGCC 1 cut(s) 1372
BsiHKAI GWGCWC 1 cut(s) 137
BsiHKCI CYCGRG 5 cut(s) 182, 245, 501, 732, 749
BsiSI CCGG 1 cut(s) 1478
BsiWI CGTACG 1 cut(s) 835
BslFI GGGAC 1 cut(s) 637
BslI CCNNNNNNNGG 2 cut(s) 620, 994
BsmAI GTCTC 2 cut(s) 498, 1050
BsmBI CGTCTC 1 cut(s) 1050
BsmFI GGGAC 1 cut(s) 637
BsmI GAATGC 3 cut(s) 447, 800, 1295
BsnI GGCC 1 cut(s) 1372
BsoBI CYCGRG 5 cut(s) 182, 245, 501, 732, 749
Bsp1286I GDGCHC 4 cut(s) 137, 207, 392, 876
Bsp1407I TGTACA 2 cut(s) 850, 1036
Bsp143I GATC 8 cut(s) 13, 49, 63, 276, 423, 627, 859, 1349
BspACI CCGC 5 cut(s) 219, 221, 1369, 1381, 1595
BspANI GGCC 1 cut(s) 1372
BspCNI CTCAG 1 cut(s) 1099
BspFNI CGCG 1 cut(s) 221
BspHI TCATGA 1 cut(s) 1074
BspLI GGNNCC 4 cut(s) 187, 225, 236, 757
BspMI ACCTGC 2 cut(s) 634, 1574
BspPI GGATC 3 cut(s) 58, 431, 1357
BspQI GCTCTTC 2 cut(s) 369, 380
BsrDI GCAATG 3 cut(s) 294, 507, 645
BsrGI TGTACA 2 cut(s) 850, 1036
BsrI ACTGG 2 cut(s) 827, 1107
BssECI CCNNGG 6 cut(s) 219, 398, 750, 808, 867, 1501
BssMI GATC 8 cut(s) 13, 49, 63, 276, 423, 627, 859, 1349
BssNI GRCGYC 2 cut(s) 326, 1043
Bst2UI CCWGG 2 cut(s) 810, 868
Bst4CI ACNGT 3 cut(s) 202, 915, 1509
Bst6I CTCTTC 3 cut(s) 369, 380, 458
BstACI GRCGYC 2 cut(s) 326, 1043
BstAUI TGTACA 2 cut(s) 850, 1036
BstC8I GCNNGC 2 cut(s) 290, 687
BstDEI CTNAG 4 cut(s) 1086, 1247, 1256, 1571
BstDSI CCRYGG 2 cut(s) 219, 1501
BstF5I GGATG 7 cut(s) 72, 117, 590, 804, 819, 1309, 1498
BstFNI CGCG 1 cut(s) 221
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 3 cut(s) 143, 301, 1357
BstKTI GATC 8 cut(s) 16, 52, 66, 279, 426, 630, 862, 1352
BstMAI GTCTC 2 cut(s) 498, 1050
BstMBI GATC 8 cut(s) 13, 49, 63, 276, 423, 627, 859, 1349
BstMWI GCNNNNNNNGC 3 cut(s) 298, 307, 1612
BstNI CCWGG 2 cut(s) 810, 868
BstNSI RCATGY 3 cut(s) 689, 1361, 1472
BstSCI CCNGG 3 cut(s) 808, 866, 1476
BstSLI GKGCMC 2 cut(s) 207, 876
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 1176
BstXI CCANNNNNNTGG 1 cut(s) 301
Bsu36I CCTNAGG 1 cut(s) 1247
BsuRI GGCC 1 cut(s) 1372
BtgI CCRYGG 2 cut(s) 219, 1501
BtsCI GGATG 7 cut(s) 72, 117, 590, 804, 819, 1309, 1498
BtsIMutI CAGTG 2 cut(s) 700, 1122
BveI ACCTGC 2 cut(s) 634, 1574
Cac8I GCNNGC 2 cut(s) 290, 687
CaiI CAGNNNCTG 1 cut(s) 1389
CciI TCATGA 1 cut(s) 1074
CfoI GCGC 3 cut(s) 143, 301, 1357
Cfr13I GGNCC 3 cut(s) 186, 223, 1498
Cfr42I CCGCGG 1 cut(s) 222
CseI GACGC 1 cut(s) 315
Csp6I GTAC 5 cut(s) 438, 836, 851, 940, 1037
CviQI GTAC 5 cut(s) 438, 836, 851, 940, 1037
DdeI CTNAG 4 cut(s) 1086, 1247, 1256, 1571
DpnI GATC 8 cut(s) 15, 51, 65, 278, 425, 629, 861, 1351
DpnII GATC 8 cut(s) 13, 49, 63, 276, 423, 627, 859, 1349
EaeI YGGCCR 1 cut(s) 1370
Eam1104I CTCTTC 3 cut(s) 369, 380, 458
EarI CTCTTC 3 cut(s) 369, 380, 458
Eco24I GRGCYC 1 cut(s) 392
Eco32I GATATC 2 cut(s) 776, 842
Eco47I GGWCC 3 cut(s) 186, 223, 1498
Eco57I CTGAAG 2 cut(s) 350, 1098
Eco81I CCTNAGG 1 cut(s) 1247
Eco88I CYCGRG 5 cut(s) 182, 245, 501, 732, 749
EcoRII CCWGG 2 cut(s) 808, 866
EcoRV GATATC 2 cut(s) 776, 842
EcoT22I ATGCAT 1 cut(s) 449
EcoT38I GRGCYC 1 cut(s) 392
Esp3I CGTCTC 1 cut(s) 1050
FalI AAGNNNNNCTT 2 cut(s) 1325, 1357
FaqI GGGAC 1 cut(s) 637
FauNDI CATATG 1 cut(s) 1054
FbaI TGATCA 1 cut(s) 859
Fnu4HI GCNGC 2 cut(s) 1165, 1370
FokI GGATG 7 cut(s) 59, 104, 577, 791, 806, 1316, 1505
FriOI GRGCYC 1 cut(s) 392
Fsp4HI GCNGC 2 cut(s) 1165, 1370
FspBI CTAG 3 cut(s) 429, 1005, 1187
GlaI GCGC 3 cut(s) 142, 300, 1356
GluI GCNGC 2 cut(s) 1165, 1370
GsuI CTGGAG 1 cut(s) 1124
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 1 cut(s) 1372
HapII CCGG 1 cut(s) 1478
HgaI GACGC 1 cut(s) 315
HhaI GCGC 3 cut(s) 143, 301, 1357
Hin1I GRCGYC 2 cut(s) 326, 1043
Hin6I GCGC 3 cut(s) 141, 299, 1355
HinP1I GCGC 3 cut(s) 141, 299, 1355
HincII GTYRAC 2 cut(s) 198, 658
HindII GTYRAC 2 cut(s) 198, 658
HindIII AAGCTT 1 cut(s) 711
HinfI GANTC 8 cut(s) 95, 596, 605, 1207, 1260, 1457, 1523, 1542
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 1 cut(s) 1478
HphI GGTGA 3 cut(s) 10, 44, 61
Hpy166II GTNNAC 7 cut(s) 198, 658, 853, 909, 1180, 1231, 1505
Hpy188III TCNNGA 6 cut(s) 247, 703, 749, 1075, 1187, 1211
Hpy8I GTNNAC 7 cut(s) 198, 658, 853, 909, 1180, 1231, 1505
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 5 cut(s) 37, 54, 370, 504, 1122
HpyCH4III ACNGT 3 cut(s) 202, 915, 1509
HpyCH4IV ACGT 4 cut(s) 1032, 1043, 1328, 1434
HpyF10VI GCNNNNNNNGC 3 cut(s) 298, 307, 1612
HpyF3I CTNAG 4 cut(s) 1086, 1247, 1256, 1571
HpySE526I ACGT 4 cut(s) 1032, 1043, 1328, 1434
Hsp92I GRCGYC 2 cut(s) 326, 1043
HspAI GCGC 3 cut(s) 141, 299, 1355
Ksp22I TGATCA 1 cut(s) 859
KspAI GTTAAC 1 cut(s) 658
KspI CCGCGG 1 cut(s) 222
Kzo9I GATC 8 cut(s) 13, 49, 63, 276, 423, 627, 859, 1349
LguI GCTCTTC 2 cut(s) 369, 380
LmnI GCTCC 2 cut(s) 517, 1105
Lsp1109I GCAGC 1 cut(s) 1176
LweI GCATC 5 cut(s) 126, 434, 599, 680, 1091
MaeI CTAG 3 cut(s) 429, 1005, 1187
MaeII ACGT 4 cut(s) 1032, 1043, 1328, 1434
MalI GATC 8 cut(s) 15, 51, 65, 278, 425, 629, 861, 1351
MboI GATC 8 cut(s) 13, 49, 63, 276, 423, 627, 859, 1349
MboII GAAGA 7 cut(s) 356, 397, 445, 547, 1551, 1565, 1646
MfeI CAATTG 1 cut(s) 582
MhlI GDGCHC 4 cut(s) 137, 207, 392, 876
MluCI AATT 8 cut(s) 282, 356, 569, 582, 922, 1094, 1394, 1447
MlyI GAGTC 2 cut(s) 1254, 1466
MmeI TCCRAC 1 cut(s) 847
Mph1103I ATGCAT 1 cut(s) 449
MroXI GAANNNNTTC 2 cut(s) 493, 712
MseI TTAA 7 cut(s) 272, 657, 1064, 1091, 1332, 1365, 1617
MslI CAYNNNNRTG 2 cut(s) 1053, 1236
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 1478
MspR9I CCNGG 3 cut(s) 810, 868, 1478
MunI CAATTG 1 cut(s) 582
Mva1269I GAATGC 3 cut(s) 447, 800, 1295
MvaI CCWGG 2 cut(s) 810, 868
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 3 cut(s) 298, 307, 1612
NciI CCSGG 1 cut(s) 1478
NdeI CATATG 1 cut(s) 1054
NdeII GATC 8 cut(s) 13, 49, 63, 276, 423, 627, 859, 1349
NlaIV GGNNCC 4 cut(s) 187, 225, 236, 757
NmeAIII GCCGAG 1 cut(s) 423
NsiI ATGCAT 1 cut(s) 449
NspI RCATGY 3 cut(s) 689, 1361, 1472
PaeI GCATGC 1 cut(s) 689
PaeR7I CTCGAG 2 cut(s) 245, 732
PagI TCATGA 1 cut(s) 1074
PciSI GCTCTTC 2 cut(s) 369, 380
PctI GAATGC 3 cut(s) 447, 800, 1295
PdmI GAANNNNTTC 2 cut(s) 493, 712
PfeI GAWTC 6 cut(s) 95, 596, 605, 1207, 1523, 1542
Pfl23II CGTACG 1 cut(s) 835
PfoI TCCNGGA 1 cut(s) 1476
PkrI GCNGC 2 cut(s) 1166, 1371
PleI GAGTC 2 cut(s) 1254, 1465
PpsI GAGTC 2 cut(s) 1254, 1465
Psp1406I AACGTT 2 cut(s) 1032, 1328
Psp6I CCWGG 2 cut(s) 808, 866
PspGI CCWGG 2 cut(s) 808, 866
PspLI CGTACG 1 cut(s) 835
PspN4I GGNNCC 4 cut(s) 187, 225, 236, 757
PspPI GGNCC 3 cut(s) 186, 223, 1498
PspXI VCTCGAGB 1 cut(s) 732
PstNI CAGNNNCTG 1 cut(s) 1389
RsaI GTAC 5 cut(s) 439, 837, 852, 941, 1038
RsaNI GTAC 5 cut(s) 438, 836, 851, 940, 1037
RseI CAYNNNNRTG 2 cut(s) 1053, 1236
SacII CCGCGG 1 cut(s) 222
SapI GCTCTTC 2 cut(s) 369, 380
SaqAI TTAA 7 cut(s) 272, 657, 1064, 1091, 1332, 1365, 1617
SatI GCNGC 2 cut(s) 1165, 1370
Sau3AI GATC 8 cut(s) 13, 49, 63, 276, 423, 627, 859, 1349
Sau96I GGNCC 3 cut(s) 186, 223, 1498
SchI GAGTC 2 cut(s) 1254, 1466
ScrFI CCNGG 3 cut(s) 810, 868, 1478
SduI GDGCHC 4 cut(s) 137, 207, 392, 876
SfaNI GCATC 5 cut(s) 126, 434, 599, 680, 1091
Sfr274I CTCGAG 2 cut(s) 245, 732
Sfr303I CCGCGG 1 cut(s) 222
SgrBI CCGCGG 1 cut(s) 222
SinI GGWCC 3 cut(s) 186, 223, 1498
SlaI CTCGAG 2 cut(s) 245, 732
SmiMI CAYNNNNRTG 2 cut(s) 1053, 1236
SmlI CTYRAG 3 cut(s) 245, 732, 1262
SmoI CTYRAG 3 cut(s) 245, 732, 1262
SpeI ACTAGT 1 cut(s) 1004
SphI GCATGC 1 cut(s) 689
Sse9I AATT 8 cut(s) 282, 356, 569, 582, 922, 1094, 1394, 1447
SsiI CCGC 5 cut(s) 219, 221, 1369, 1381, 1595
SspMI CTAG 3 cut(s) 429, 1005, 1187
StyD4I CCNGG 3 cut(s) 808, 866, 1476
TaaI ACNGT 3 cut(s) 202, 915, 1509
TaiI ACGT 4 cut(s) 1035, 1046, 1331, 1437
TaqI TCGA 3 cut(s) 246, 442, 733
TasI AATT 8 cut(s) 282, 356, 569, 582, 922, 1094, 1394, 1447
TatI WGTACW 3 cut(s) 437, 850, 1036
TauI GCSGC 1 cut(s) 1372
TfiI GAWTC 6 cut(s) 95, 596, 605, 1207, 1523, 1542
Tru1I TTAA 7 cut(s) 272, 657, 1064, 1091, 1332, 1365, 1617
Tru9I TTAA 7 cut(s) 272, 657, 1064, 1091, 1332, 1365, 1617
TscAI CASTG 2 cut(s) 700, 1122
TseI GCWGC 1 cut(s) 1164
TspDTI ATGAA 9 cut(s) 582, 705, 774, 793, 916, 1041, 1091, 1220, 1288
TspGWI ACGGA 2 cut(s) 496, 1490
TspRI CASTG 2 cut(s) 700, 1122
VpaK11BI GGWCC 3 cut(s) 186, 223, 1498
XapI RAATTY 2 cut(s) 282, 1447
XbaI TCTAGA 1 cut(s) 1186
XceI RCATGY 3 cut(s) 689, 1361, 1472
XhoI CTCGAG 2 cut(s) 245, 732
XmnI GAANNNNTTC 2 cut(s) 493, 712
XspI CTAG 3 cut(s) 429, 1005, 1187
ZraI GACGTC 1 cut(s) 1044
Zsp2I ATGCAT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.