Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0009625

Physical Location & Seq

Genomic Coordinates Reverse (-)

18596865 .. 18605384

Gene Structure 8520 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2136 bp

ATGACATCAATCATTTTGTTGGGCTCCTTTCATCATACAAGGTTATTGCATGTTCGTCGGGAAGCAAATCAGACTCAAAACTCAAAATTTGCAGCCTACCAAAGTACACAGATCACACTCATTGTCATCTGTCACAACCTAACATATGCTTTTGAGCAAAAACATTCAGAGGAAAAAAAGAAGAAAAAAGTTTCTTTCTTTTTCTCTGTGAAAAAGAAATCAAAAATGGCGCTCTCACGGCTTCGCCACCCAGTGATCTCCCGCGCGCCGTTTCTCCTGAGAGCTCGACTTCTCTCCTCGTCCACCAGATCACTCACTCGTACTTTCAGTGTGCAAAATTCTGCTGAGCTTGGTGATGATATGCTGTTAAGGCCAGCAACTTTGTCCATGCTCACTGGAGTTCATGATAAACCATCAAAGCTGAAGCCTTTGACGGGTGTCAAGTATTATTCAACTCAAGATCCTTTGCATACTGTCATTGGGATGCCGGCGTTGTCTCCTACAATGACTCAAGGTAACATTGCAAAGTGGTGGAAGAAAGAAGGAGACAAGATTGAACCGGGCGACATACTATGTGAAATTGAAACTGACAAAGCTACTATTGAATTTGAAAGTCTTGAAGAAGGGTTTCTAGCCAAGATATTGGCACCTGAAGGTTCAAAAGATGTGCCTGTGGGACAGCCTATTGCAATAACGGTTGAGGATGCAGATGATATCCAAAACCTCCCTGCTAATGTGGTCGGTGGATCTGAGGTCAAAGAGGATATACCAGCACATCAGAATGTCAGAAAGGAAGAGGGGGTACAAGATACAAGTTCTGTGGGCATAAACACATCAGAACTTCCTCCTCACATTGTCATTGAAATGCCAGCTTTATCACCAACAATGAGCCAGGGAAACATTTTTAAGTGGACAAAGAAAGAAGGAGATAAGATAGAAGTGGGTGATATAATATGTGAGATAGAGACAGACAAAGCAACCATTGAATTTGAATGTCTTGAAGAGGGATACTTGGCTAAGATACTAGCGCCAGACGGTACAAAGGATATAGCTGTTGGCCAACCAATTGCAGTAACAGTTGAGGATGCATCTGATCTGGAAACTGTAAAGAATGCTGTTAGCAGTGGTTCACCCGTCAAAGAAGAAACACCAGTCCGTCAGGATACCGGAGATGTAACTAGATCGTTAAAAACCAGTATTTCAAGGATCAGTCCAGCAGCAAAGTTGCTTATTACGGAACATGGATTGGATACATCATTGCTGAAGGCATCAGGTCCTCATGGTACATTGCTGAAAGGGGATGTTTTAGCTGCAATCAAGTCAGGAATAGGGTCTTCAAAAGTTTCATCCAAAGAAAAGACTACTCCATCACCGTCAAAGGCATCAGGTGCTCATGTCCCGAAACCACAAAAGTCAAAACCTTCAAAACAGACAGATTCTTTTGAAGATTTTCCCAATAGTCAAATCCGCAAGGTGATAGCTACTAGACTGCTAGAATCAAAACAAAACATACCGCATTTATATTTATCATCAGATGTTATACTGGACCCTCTTCTTTCTCTTAGAAGGGATCTTATAGAACAGCATAATGTTAAAGTTTCAGTTAATGACATTGTTATCAAAGCTGTAGCTGTTGCACTGAAAAATGTACCGGAAGCAAATGCTTACTGGGATGCTGAAAAAGGAGAAGTCTTTCTTTGTGATTCCGTTGATATATCAATTGCTGTGGCCACTGAGAAGGGCCTGATGACTCCAATTGTGAAGAATGCAGATCAGAAATCAATATCAGCAATCTCTGCAGAGGTCAAGGAACTAGCTGAGAAGGCACGAGCGGGAAAGCTTAAGCCAAATGAATTCCAAGGAGGAACTTTCAGTATTTCAAACTTGGGGATGTTTCCAGTGGATAATTTCTGTGCAATTATAAACCCACCTCAGGCCAGTATCCTTGCTGTTGGCAGAGGCAACAAAGTAGTTGAACCAGTGATTGGAAGCGACGGAATTGAGAGGCCGGCAGTTGTCACCAAAATGAACTTGACATTATCGGCTGATCATCGAGTATTTGACGGAAAAGTTGGAGGTTCGTTCCTCTCAGCTTTACGTTCAAACTTCAGTGATATCCGAAGGCTTCTTTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0032991 GO:0044424 GO:0044444 GO:0044464 GO:0045254 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200

Pfam Domains

Protein Families

2-oxoacid_dh Biotin_lipoyl E3_binding

Protein Analysis

712

Amino Acids

77.23

Weight (kDa)

7.65

Isoelectric Point (pI)

46.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_lipoyl	PF00364	159 - 230	6.5e-18	Biotin-requiring enzyme
Biotin_lipoyl	PF00364	287 - 358	2.9e-17	Biotin-requiring enzyme
E3_binding	PF02817	402 - 436	4.3e-08	e3 binding domain
2-oxoacid_dh	PF00198	481 - 710	6.6e-74	2-oxoacid dehydrogenases acyltransferase (catalytic domain)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009625)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G52200 AT3G52200
fragaria_vesca	FvH4_7g18300 FvH4_7g18300 FvH4_7g18300
malus_domestica	MD01G1099700.v1.1 MD07G1166600.v1.1
prunus_persica	Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1 Prupe.2G204600_v2.0.a1
pyrus_communis	pycom01g12680 pycom07g16180
rosa_chinensis	RchiOBHm_Chr1g0361921
rosa_laevigata	RLG00000027686
rosa_multiflora	Rmu_sc0008041.1_g000008
rosa_roxburghii	Rroxscaffold_4G00294620
rosa_rugosa	Rorug01G0292400
rosa_samantha	Rh1AG302900 Rh1CG284300 Rh1DG296100
rosa_wichuraiana	Rw1G026840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1922
AccB1I	GGYRCC	1 cut(s)	646
AccB7I	CCANNNNNTGG	1 cut(s)	1985
AccBSI	CCGCTC	1 cut(s)	1832
AccII	CGCG	2 cut(s)	264, 266
AciI	CCGC	4 cut(s)	262, 1468, 1514, 1832
AclWI	GGATC	4 cut(s)	455, 754, 1214, 1578
AcoI	YGGCCR	2 cut(s)	1057, 1728
AcsI	RAATTY	5 cut(s)	86, 337, 605, 986, 1853
AcuI	CTGAAG	4 cut(s)	443, 672, 1283, 2092
AdeI	CACNNNGTG	1 cut(s)	253
AfaI	GTAC	6 cut(s)	106, 322, 804, 1039, 1285, 1650
AfiI	CCNNNNNNNGG	3 cut(s)	434, 1933, 1985
AflII	CTTAAG	1 cut(s)	1841
AjnI	CCWGG	1 cut(s)	891
AjuI	GAANNNNNNNTTGG	2 cut(s)	1968, 2000
Alw21I	GWGCWC	2 cut(s)	286, 1393
Alw26I	GTCTC	3 cut(s)	501, 540, 959
AlwI	GGATC	4 cut(s)	455, 754, 1214, 1578
AoxI	GGCC	6 cut(s)	371, 1057, 1728, 1741, 1935, 2006
ApeKI	GCWGC	3 cut(s)	92, 1217, 1310
ApoI	RAATTY	5 cut(s)	86, 337, 605, 986, 1853
Asp700I	GAANNNNTTC	3 cut(s)	627, 1449, 1692
AspLEI	GCGC	4 cut(s)	232, 266, 268, 1030
AspS9I	GGNCC	3 cut(s)	1274, 1546, 1741
AsuC2I	CCSGG	1 cut(s)	561
AsuHPI	GGTGA	7 cut(s)	365, 870, 956, 1122, 1362, 1486, 2011
AvaII	GGWCC	2 cut(s)	1274, 1546
AxyI	CCTNAGG	1 cut(s)	1932
BalI	TGGCCA	2 cut(s)	1059, 1730
BanI	GGYRCC	1 cut(s)	646
BanII	GRGCYC	2 cut(s)	26, 286
BarI	GAAGNNNNNNTAC	2 cut(s)	786, 818
BauI	CACGAG	1 cut(s)	1827
BbsI	GAAGAC	1 cut(s)	1326
Bbv12I	GWGCWC	2 cut(s)	286, 1393
BbvI	GCAGC	3 cut(s)	104, 1229, 1297
BccI	CCATC	2 cut(s)	421, 1375
BceAI	ACGGC	2 cut(s)	253, 254
BcgI	CGANNNNNNTGC	2 cut(s)	38, 72
BciT130I	CCWGG	1 cut(s)	893
BciVI	GTATCC	4 cut(s)	1001, 1156, 1243, 1952
BclI	TGATCA	1 cut(s)	2047
BcnI	CCSGG	1 cut(s)	561
BcoDI	GTCTC	3 cut(s)	501, 540, 959
BfaI	CTAG	6 cut(s)	632, 1025, 1179, 1485, 1493, 1814
BfmI	CTRYAG	2 cut(s)	1626, 1797
BfoI	RGCGCY	2 cut(s)	233, 1031
BfrI	CTTAAG	1 cut(s)	1841
BfuI	GTATCC	4 cut(s)	1001, 1156, 1243, 1952
BisI	GCNGC	3 cut(s)	93, 1218, 1311
BlpI	GCTNAGC	1 cut(s)	345
BlsI	GCNGC	3 cut(s)	94, 1219, 1312
Bme1390I	CCNGG	2 cut(s)	561, 893
Bme18I	GGWCC	2 cut(s)	1274, 1546
BmgT120I	GGNCC	3 cut(s)	1274, 1546, 1741
BmiI	GGNNCC	3 cut(s)	25, 648, 1548
BmrFI	CCNGG	2 cut(s)	561, 893
BmrI	ACTGGG	2 cut(s)	245, 1678
BmsI	GCATC	7 cut(s)	474, 694, 1075, 1097, 1277, 1391, 1663
BmuI	ACTGGG	2 cut(s)	245, 1678
BoxI	GACNNNNGTC	1 cut(s)	437
BpiI	GAAGAC	1 cut(s)	1326
BpmI	CTGGAG	1 cut(s)	417
Bpu1102I	GCTNAGC	1 cut(s)	345
BpuEI	CTTGAG	2 cut(s)	441, 495
BpuMI	CCSGG	1 cut(s)	561
BsaJI	CCNNGG	2 cut(s)	892, 1858
BsaWI	WCCGGW	2 cut(s)	1166, 1651
Bsc4I	CCNNNNNNNGG	3 cut(s)	434, 1933, 1985
Bse118I	RCCGGY	2 cut(s)	487, 2008
Bse1I	ACTGG	9 cut(s)	251, 400, 1151, 1194, 1548, 1673, 1898, 1938, 1979
Bse21I	CCTNAGG	1 cut(s)	1932
Bse3DI	GCAATG	3 cut(s)	519, 1256, 1286
BseBI	CCWGG	1 cut(s)	893
BseDI	CCNNGG	2 cut(s)	892, 1858
BseGI	GGATG	7 cut(s)	489, 709, 1090, 1306, 1346, 1678, 1896
BseLI	CCNNNNNNNGG	3 cut(s)	434, 1933, 1985
BseMI	GCAATG	3 cut(s)	519, 1256, 1286
BseMII	CTCAG	7 cut(s)	269, 336, 741, 1725, 1809, 1946, 2103
BseNI	ACTGG	9 cut(s)	251, 400, 1151, 1194, 1548, 1673, 1898, 1938, 1979
BsePI	GCGCGC	1 cut(s)	264
BseRI	GAGGAG	2 cut(s)	286, 837
BseXI	GCAGC	3 cut(s)	104, 1229, 1297
Bsh1236I	CGCG	2 cut(s)	264, 266
BshFI	GGCC	6 cut(s)	373, 1059, 1730, 1743, 1937, 2008
BshNI	GGYRCC	1 cut(s)	646
BsiHKAI	GWGCWC	2 cut(s)	286, 1393
BsiSI	CCGG	5 cut(s)	488, 560, 1167, 1652, 2009
BslFI	GGGAC	2 cut(s)	690, 1382
BslI	CCNNNNNNNGG	3 cut(s)	434, 1933, 1985
BsmAI	GTCTC	3 cut(s)	501, 540, 959
BsmFI	GGGAC	2 cut(s)	690, 1382
BsmI	GAATGC	2 cut(s)	1117, 1771
BsnI	GGCC	6 cut(s)	373, 1059, 1730, 1743, 1937, 2008
Bsp1286I	GDGCHC	3 cut(s)	26, 286, 1393
Bsp1720I	GCTNAGC	1 cut(s)	345
BspACI	CCGC	4 cut(s)	262, 1468, 1514, 1832
BspANI	GGCC	6 cut(s)	373, 1059, 1730, 1743, 1937, 2008
BspCNI	CTCAG	7 cut(s)	270, 337, 742, 1726, 1810, 1945, 2102
BspFNI	CGCG	2 cut(s)	264, 266
BspHI	TCATGA	1 cut(s)	403
BspLI	GGNNCC	3 cut(s)	25, 648, 1548
BspMAI	CTGCAG	1 cut(s)	1801
BspPI	GGATC	4 cut(s)	455, 754, 1214, 1578
BspT107I	GGYRCC	1 cut(s)	646
BspTI	CTTAAG	1 cut(s)	1841
BsrBI	CCGCTC	1 cut(s)	1832
BsrDI	GCAATG	3 cut(s)	519, 1256, 1286
BsrFI	RCCGGY	2 cut(s)	487, 2008
BsrI	ACTGG	9 cut(s)	251, 400, 1151, 1194, 1548, 1673, 1898, 1938, 1979
BssAI	RCCGGY	2 cut(s)	487, 2008
BssECI	CCNNGG	2 cut(s)	892, 1858
BssHII	GCGCGC	1 cut(s)	264
BssSI	CACGAG	1 cut(s)	1827
BssT1I	CCWWGG	1 cut(s)	1858
Bst2BI	CACGAG	1 cut(s)	1827
Bst2UI	CCWGG	1 cut(s)	893
Bst4CI	ACNGT	6 cut(s)	475, 697, 1037, 1078, 1105, 1374
Bst6I	CTCTTC	3 cut(s)	789, 996, 1557
BstAFI	CTTAAG	1 cut(s)	1841
BstAPI	GCANNNNNTGC	2 cut(s)	1388, 1796
BstC8I	GCNNGC	5 cut(s)	266, 375, 489, 870, 2010
BstDEI	CTNAG	9 cut(s)	278, 345, 750, 1017, 1562, 1734, 1818, 1932, 2089
BstF5I	GGATG	7 cut(s)	489, 709, 1090, 1306, 1346, 1678, 1896
BstFNI	CGCG	2 cut(s)	264, 266
BstH2I	RGCGCY	2 cut(s)	233, 1031
BstHHI	GCGC	4 cut(s)	232, 266, 268, 1030
BstMAI	GTCTC	3 cut(s)	501, 540, 959
BstMWI	GCNNNNNNNGC	5 cut(s)	238, 370, 1388, 1796, 1823
BstNI	CCWGG	1 cut(s)	893
BstNSI	RCATGY	1 cut(s)	53
BstPAI	GACNNNNGTC	1 cut(s)	437
BstSCI	CCNGG	2 cut(s)	559, 891
BstSFI	CTRYAG	2 cut(s)	1626, 1797
BstUI	CGCG	2 cut(s)	264, 266
BstV1I	GCAGC	3 cut(s)	104, 1229, 1297
BstV2I	GAAGAC	1 cut(s)	1326
BstX2I	RGATCY	3 cut(s)	460, 746, 1570
BstXI	CCANNNNNNTGG	1 cut(s)	643
BstYI	RGATCY	3 cut(s)	460, 746, 1570
Bsu36I	CCTNAGG	1 cut(s)	1932
BsuI	GTATCC	4 cut(s)	1001, 1156, 1243, 1952
BsuRI	GGCC	6 cut(s)	373, 1059, 1730, 1743, 1937, 2008
BtsCI	GGATG	7 cut(s)	489, 709, 1090, 1306, 1346, 1678, 1896
BtsI	GCAGTG	1 cut(s)	1129
BtsIMutI	CAGTG	9 cut(s)	258, 334, 393, 1129, 1637, 1731, 1905, 1986, 2116
Cac8I	GCNNGC	5 cut(s)	266, 375, 489, 870, 2010
CciI	TCATGA	1 cut(s)	403
CfoI	GCGC	4 cut(s)	232, 266, 268, 1030
Cfr10I	RCCGGY	2 cut(s)	487, 2008
Cfr13I	GGNCC	3 cut(s)	1274, 1546, 1741
Csp6I	GTAC	6 cut(s)	105, 321, 803, 1038, 1284, 1649
CspCI	CAANNNNNGTGG	4 cut(s)	801, 836, 1707, 1742
CviAII	CATG	6 cut(s)	50, 388, 404, 1241, 1280, 1394
CviQI	GTAC	6 cut(s)	105, 321, 803, 1038, 1284, 1649
DdeI	CTNAG	9 cut(s)	278, 345, 750, 1017, 1562, 1734, 1818, 1932, 2089
DraIII	CACNNNGTG	1 cut(s)	253
EaeI	YGGCCR	2 cut(s)	1057, 1728
Eam1104I	CTCTTC	3 cut(s)	789, 996, 1557
EarI	CTCTTC	3 cut(s)	789, 996, 1557
Ecl136II	GAGCTC	1 cut(s)	284
Eco130I	CCWWGG	1 cut(s)	1858
Eco24I	GRGCYC	2 cut(s)	26, 286
Eco32I	GATATC	2 cut(s)	715, 2116
Eco47I	GGWCC	2 cut(s)	1274, 1546
Eco53kI	GAGCTC	1 cut(s)	284
Eco57I	CTGAAG	4 cut(s)	443, 672, 1283, 2092
Eco81I	CCTNAGG	1 cut(s)	1932
EcoICRI	GAGCTC	1 cut(s)	284
EcoO109I	RGGNCCY	2 cut(s)	1274, 1741
EcoRI	GAATTC	1 cut(s)	1853
EcoRII	CCWGG	1 cut(s)	891
EcoRV	GATATC	2 cut(s)	715, 2116
EcoT14I	CCWWGG	1 cut(s)	1858
EcoT22I	ATGCAT	1 cut(s)	1090
EcoT38I	GRGCYC	2 cut(s)	26, 286
ErhI	CCWWGG	1 cut(s)	1858
FaeI	CATG	6 cut(s)	53, 391, 407, 1244, 1283, 1397
FalI	AAGNNNNNCTT	2 cut(s)	1680, 1712
FaqI	GGGAC	2 cut(s)	690, 1382
FatI	CATG	6 cut(s)	49, 387, 403, 1240, 1279, 1393
FauI	CCCGC	2 cut(s)	269, 1825
FauNDI	CATATG	1 cut(s)	145
FbaI	TGATCA	1 cut(s)	2047
Fnu4HI	GCNGC	3 cut(s)	93, 1218, 1311
FokI	GGATG	7 cut(s)	496, 716, 1097, 1313, 1333, 1685, 1903
FriOI	GRGCYC	2 cut(s)	26, 286
Fsp4HI	GCNGC	3 cut(s)	93, 1218, 1311
FspBI	CTAG	6 cut(s)	632, 1025, 1179, 1485, 1493, 1814
GlaI	GCGC	4 cut(s)	231, 265, 267, 1029
GluI	GCNGC	3 cut(s)	93, 1218, 1311
GsuI	CTGGAG	1 cut(s)	417
HaeII	RGCGCY	2 cut(s)	233, 1031
HaeIII	GGCC	6 cut(s)	373, 1059, 1730, 1743, 1937, 2008
HapII	CCGG	5 cut(s)	488, 560, 1167, 1652, 2009
HhaI	GCGC	4 cut(s)	232, 266, 268, 1030
Hin1II	CATG	6 cut(s)	53, 391, 407, 1244, 1283, 1397
Hin6I	GCGC	4 cut(s)	230, 264, 266, 1028
HinP1I	GCGC	4 cut(s)	230, 264, 266, 1028
HindIII	AAGCTT	1 cut(s)	1838
HinfI	GANTC	6 cut(s)	73, 508, 1436, 1496, 1703, 1750
HpaII	CCGG	5 cut(s)	488, 560, 1167, 1652, 2009
HphI	GGTGA	7 cut(s)	365, 870, 956, 1122, 1362, 1486, 2011
Hpy166II	GTNNAC	4 cut(s)	107, 303, 912, 1130
Hpy8I	GTNNAC	4 cut(s)	107, 303, 912, 1130
Hpy99I	CGWCG	2 cut(s)	60, 1997
HpyCH4III	ACNGT	6 cut(s)	475, 697, 1037, 1078, 1105, 1374
HpyCH4IV	ACGT	1 cut(s)	2098
HpyF10VI	GCNNNNNNNGC	5 cut(s)	238, 370, 1388, 1796, 1823
HpyF3I	CTNAG	9 cut(s)	278, 345, 750, 1017, 1562, 1734, 1818, 1932, 2089
HpySE526I	ACGT	1 cut(s)	2098
Hsp92II	CATG	6 cut(s)	53, 391, 407, 1244, 1283, 1397
HspAI	GCGC	4 cut(s)	230, 264, 266, 1028
KroI	GCCGGC	2 cut(s)	487, 2008
KroNI	GCCGGC	2 cut(s)	489, 2010
Ksp22I	TGATCA	1 cut(s)	2047
LmnI	GCTCC	1 cut(s)	29
Lsp1109I	GCAGC	3 cut(s)	104, 1229, 1297
LweI	GCATC	7 cut(s)	474, 694, 1075, 1097, 1277, 1391, 1663
MaeI	CTAG	6 cut(s)	632, 1025, 1179, 1485, 1493, 1814
MaeII	ACGT	1 cut(s)	2098
MaeIII	GTNAC	5 cut(s)	131, 515, 1072, 1174, 2017
MbiI	CCGCTC	1 cut(s)	1832
MfeI	CAATTG	3 cut(s)	1065, 1719, 1755
MflI	RGATCY	3 cut(s)	460, 746, 1570
MhlI	GDGCHC	3 cut(s)	26, 286, 1393
MlsI	TGGCCA	2 cut(s)	1059, 1730
MluNI	TGGCCA	2 cut(s)	1059, 1730
MlyI	GAGTC	3 cut(s)	67, 502, 1744
MmeI	TCCRAC	1 cut(s)	2053
Mox20I	TGGCCA	2 cut(s)	1059, 1730
Mph1103I	ATGCAT	1 cut(s)	1090
MroNI	GCCGGC	2 cut(s)	487, 2008
MroXI	GAANNNNTTC	3 cut(s)	627, 1449, 1692
MscI	TGGCCA	2 cut(s)	1059, 1730
MseI	TTAA	6 cut(s)	368, 906, 1187, 1593, 1605, 1842
MslI	CAYNNNNRTG	4 cut(s)	482, 780, 863, 2024
Msp20I	TGGCCA	2 cut(s)	1059, 1730
MspCI	CTTAAG	1 cut(s)	1841
MspI	CCGG	5 cut(s)	488, 560, 1167, 1652, 2009
MspR9I	CCNGG	2 cut(s)	561, 893
MunI	CAATTG	3 cut(s)	1065, 1719, 1755
Mva1269I	GAATGC	2 cut(s)	1117, 1771
MvaI	CCWGG	1 cut(s)	893
MvnI	CGCG	2 cut(s)	264, 266
MwoI	GCNNNNNNNGC	5 cut(s)	238, 370, 1388, 1796, 1823
NaeI	GCCGGC	2 cut(s)	489, 2010
NciI	CCSGG	1 cut(s)	561
NdeI	CATATG	1 cut(s)	145
NgoMIV	GCCGGC	2 cut(s)	487, 2008
NlaIII	CATG	6 cut(s)	53, 391, 407, 1244, 1283, 1397
NlaIV	GGNNCC	3 cut(s)	25, 648, 1548
NmuCI	GTSAC	2 cut(s)	131, 2017
NsiI	ATGCAT	1 cut(s)	1090
NspI	RCATGY	1 cut(s)	53
PagI	TCATGA	1 cut(s)	403
PauI	GCGCGC	1 cut(s)	264
PctI	GAATGC	2 cut(s)	1117, 1771
PdiI	GCCGGC	2 cut(s)	489, 2010
PdmI	GAANNNNTTC	3 cut(s)	627, 1449, 1692
PfeI	GAWTC	3 cut(s)	1436, 1496, 1703
PflMI	CCANNNNNTGG	1 cut(s)	1985
PkrI	GCNGC	3 cut(s)	94, 1219, 1312
PleI	GAGTC	3 cut(s)	67, 502, 1744
PpsI	GAGTC	3 cut(s)	67, 502, 1744
PpuMI	RGGWCCY	1 cut(s)	1274
PshAI	GACNNNNGTC	1 cut(s)	437
PsiI	TTATAA	1 cut(s)	1922
Psp124BI	GAGCTC	1 cut(s)	286
Psp5II	RGGWCCY	1 cut(s)	1274
Psp6I	CCWGG	1 cut(s)	891
PspGI	CCWGG	1 cut(s)	891
PspN4I	GGNNCC	3 cut(s)	25, 648, 1548
PspPI	GGNCC	3 cut(s)	1274, 1546, 1741
PspPPI	RGGWCCY	1 cut(s)	1274
PstI	CTGCAG	1 cut(s)	1801
PsuI	RGATCY	3 cut(s)	460, 746, 1570
PteI	GCGCGC	1 cut(s)	264
RsaI	GTAC	6 cut(s)	106, 322, 804, 1039, 1285, 1650
RsaNI	GTAC	6 cut(s)	105, 321, 803, 1038, 1284, 1649
RseI	CAYNNNNRTG	4 cut(s)	482, 780, 863, 2024
SacI	GAGCTC	1 cut(s)	286
SaqAI	TTAA	6 cut(s)	368, 906, 1187, 1593, 1605, 1842
SatI	GCNGC	3 cut(s)	93, 1218, 1311
Sau96I	GGNCC	3 cut(s)	1274, 1546, 1741
SchI	GAGTC	3 cut(s)	67, 502, 1744
ScrFI	CCNGG	2 cut(s)	561, 893
SduI	GDGCHC	3 cut(s)	26, 286, 1393
SfaNI	GCATC	7 cut(s)	474, 694, 1075, 1097, 1277, 1391, 1663
SfcI	CTRYAG	2 cut(s)	1626, 1797
SinI	GGWCC	2 cut(s)	1274, 1546
SmiMI	CAYNNNNRTG	4 cut(s)	482, 780, 863, 2024
SmlI	CTYRAG	3 cut(s)	456, 510, 1841
SmoI	CTYRAG	3 cut(s)	456, 510, 1841
SsiI	CCGC	4 cut(s)	262, 1468, 1514, 1832
SspMI	CTAG	6 cut(s)	632, 1025, 1179, 1485, 1493, 1814
SstI	GAGCTC	1 cut(s)	286
StyD4I	CCNGG	2 cut(s)	559, 891
StyI	CCWWGG	1 cut(s)	1858
TaaI	ACNGT	6 cut(s)	475, 697, 1037, 1078, 1105, 1374
TaiI	ACGT	1 cut(s)	2101
TaqI	TCGA	2 cut(s)	286, 2053
TatI	WGTACW	1 cut(s)	104
TfiI	GAWTC	3 cut(s)	1436, 1496, 1703
Tru1I	TTAA	6 cut(s)	368, 906, 1187, 1593, 1605, 1842
Tru9I	TTAA	6 cut(s)	368, 906, 1187, 1593, 1605, 1842
TscAI	CASTG	9 cut(s)	258, 334, 400, 1129, 1644, 1738, 1905, 1986, 2116
TseFI	GTSAC	2 cut(s)	131, 2017
TseI	GCWGC	3 cut(s)	92, 1217, 1310
Tsp45I	GTSAC	2 cut(s)	131, 2017
TspDTI	ATGAA	5 cut(s)	20, 392, 1335, 1866, 2042
TspGWI	ACGGA	5 cut(s)	1145, 1250, 1696, 2010, 2079
TspRI	CASTG	9 cut(s)	258, 334, 400, 1129, 1644, 1738, 1905, 1986, 2116
Van91I	CCANNNNNTGG	1 cut(s)	1985
Vha464I	CTTAAG	1 cut(s)	1841
VpaK11BI	GGWCC	2 cut(s)	1274, 1546
XapI	RAATTY	5 cut(s)	86, 337, 605, 986, 1853
XceI	RCATGY	1 cut(s)	53
XmnI	GAANNNNTTC	3 cut(s)	627, 1449, 1692
XspI	CTAG	6 cut(s)	632, 1025, 1179, 1485, 1493, 1814
Zsp2I	ATGCAT	1 cut(s)	1090

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.