pycom07g19000

Thylakoid lumenal 15 kDa

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0009320
Physical Location & Seq
Forward (+)
21341313 .. 21343870
2558 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g19000.1

Sequence Viewer

Length: 660 bp
ATGGCGCTTCTCAACGTCTCCATCTGCTCTTCCAAACTCCCCCCAAAACCCTCCCTCTCTCTCCCCCATAAACCTCACCCTTCCCGCCAACACCTCCCTCTCTCCGCCGGCTATCCTCCGCCCCAGGTTGTAAAGAATCGCCTGGAAACTTCTGGAAGTTCTGTTTTGTCGCTGGGAGAGTCCGTCTGTAAGGCGAGCCTTATCGCTCTGCTCTCGGCCTCTCTGTTCGTAGCCAATCCTGCATTCGCCTTTAAGGGTGGGGGTCCGTACGGTGCGGAGGTGACTAGGGGCCAGGACCTCACCGGCAAGGACTTTAGCGGCAAGACTTTGATCAAGCAAGACTTCAAAACGTCGATATTGAGGCAAGCGAATTTCAAAGGCGCCAAGTTATTAGGTGCTAGCTTCTTTGATGCTGATTTAACAGATCTAAGAGCTGTGGACTTTTCTTTGGCCAATGTGACAAAGGCGAATCTGAGCAACGCTAACTTAGAAGGTGCAGTAGCCACAGGCAACACATCTTTCAAAGGGTCAAATATTACGGGTGCAGACTTCACAGATGTGCCTCTGAGAGAAGATCAACGGGAATATCTCTGCAAAGTTGCCGATGGGGTGAATCCGACAACTGGAAACCCAACACGTGAAACCTTGCTTTGCAATTAA

Protein Analysis

220

Amino Acids

23.11

Weight (kDa)

9.06

Isoelectric Point (pI)

19.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pentapeptide PF00805 147 - 186 5.8e-07 Pentapeptide repeats (8 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 380
AciI CCGC 5 cut(s) 85, 105, 119, 275, 318
AcoI YGGCCR 1 cut(s) 450
AcsI RAATTY 1 cut(s) 370
AcvI CACGTG 1 cut(s) 638
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 623
AflIII ACRYGT 1 cut(s) 635
AgsI TTSAA 3 cut(s) 346, 376, 523
AjnI CCWGG 3 cut(s) 123, 141, 291
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AleI CACNNNNGTG 1 cut(s) 557
AluBI AGCT 2 cut(s) 402, 434
AluI AGCT 2 cut(s) 402, 434
Alw26I GTCTC 1 cut(s) 22
AoxI GGCC 3 cut(s) 216, 289, 450
ApoI RAATTY 1 cut(s) 370
AspLEI GCGC 2 cut(s) 7, 383
AspS9I GGNCC 3 cut(s) 263, 289, 295
AsuHPI GGTGA 4 cut(s) 68, 292, 292, 622
AsuNHI GCTAGC 1 cut(s) 398
AvaII GGWCC 2 cut(s) 263, 295
BalI TGGCCA 1 cut(s) 452
BanI GGYRCC 1 cut(s) 380
BbrPI CACGTG 1 cut(s) 638
BccI CCATC 2 cut(s) 29, 599
BciT130I CCWGG 3 cut(s) 125, 143, 293
BclI TGATCA 1 cut(s) 330
BcoDI GTCTC 1 cut(s) 22
BfaI CTAG 2 cut(s) 285, 399
BfoI RGCGCY 2 cut(s) 8, 384
BglII AGATCT 1 cut(s) 424
BisI GCNGC 1 cut(s) 319
BlsI GCNGC 1 cut(s) 320
Bme1390I CCNGG 3 cut(s) 125, 143, 293
Bme18I GGWCC 2 cut(s) 263, 295
BmgT120I GGNCC 3 cut(s) 263, 289, 295
BmiI GGNNCC 3 cut(s) 264, 290, 382
BmrFI CCNGG 3 cut(s) 125, 143, 293
BmsI GCATC 1 cut(s) 400
BmtI GCTAGC 1 cut(s) 402
BsaAI YACGTR 1 cut(s) 638
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 123
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse118I RCCGGY 2 cut(s) 107, 302
Bse1I ACTGG 1 cut(s) 628
BseBI CCWGG 3 cut(s) 125, 143, 293
BseDI CCNNGG 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMII CTCAG 2 cut(s) 464, 557
BseNI ACTGG 1 cut(s) 628
BseYI CCCAGC 1 cut(s) 172
BsgI GTGCAG 2 cut(s) 516, 564
BshFI GGCC 3 cut(s) 218, 291, 452
BshNI GGYRCC 1 cut(s) 380
BsiSI CCGG 2 cut(s) 108, 303
BsiWI CGTACG 1 cut(s) 267
BslI CCNNNNNNNGG 1 cut(s) 623
BsmAI GTCTC 1 cut(s) 22
BsmBI CGTCTC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 3 cut(s) 218, 291, 452
Bsp143I GATC 3 cut(s) 330, 424, 574
BspACI CCGC 5 cut(s) 85, 105, 119, 275, 318
BspANI GGCC 3 cut(s) 218, 291, 452
BspCNI CTCAG 2 cut(s) 465, 558
BspLI GGNNCC 3 cut(s) 264, 290, 382
BspOI GCTAGC 1 cut(s) 402
BspQI GCTCTTC 1 cut(s) 34
BspT107I GGYRCC 1 cut(s) 380
BsrFI RCCGGY 2 cut(s) 107, 302
BsrI ACTGG 1 cut(s) 628
BssAI RCCGGY 2 cut(s) 107, 302
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 3 cut(s) 330, 424, 574
BssNI GRCGYC 1 cut(s) 381
Bst2UI CCWGG 3 cut(s) 125, 143, 293
Bst4CI ACNGT 1 cut(s) 272
Bst6I CTCTTC 1 cut(s) 34
BstACI GRCGYC 1 cut(s) 381
BstBAI YACGTR 1 cut(s) 638
BstC8I GCNNGC 4 cut(s) 109, 196, 366, 400
BstDEI CTNAG 4 cut(s) 428, 473, 487, 566
BstH2I RGCGCY 2 cut(s) 8, 384
BstHHI GCGC 2 cut(s) 7, 383
BstKTI GATC 3 cut(s) 333, 427, 577
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 3 cut(s) 330, 424, 574
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 3 cut(s) 125, 143, 293
BstSCI CCNGG 3 cut(s) 123, 141, 291
BstX2I RGATCY 1 cut(s) 424
BstYI RGATCY 1 cut(s) 424
BsuRI GGCC 3 cut(s) 218, 291, 452
Cac8I GCNNGC 4 cut(s) 109, 196, 366, 400
CfoI GCGC 2 cut(s) 7, 383
Cfr10I RCCGGY 2 cut(s) 107, 302
Cfr13I GGNCC 3 cut(s) 263, 289, 295
Csp6I GTAC 1 cut(s) 268
CviJI RGCY 9 cut(s) 111, 198, 218, 233, 291, 402, 434, 452, 503
CviKI_1 RGCY 9 cut(s) 111, 198, 218, 233, 291, 402, 434, 452, 503
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 4 cut(s) 428, 473, 487, 566
DinI GGCGCC 1 cut(s) 382
DpnI GATC 3 cut(s) 332, 426, 576
DpnII GATC 3 cut(s) 330, 424, 574
EaeI YGGCCR 1 cut(s) 450
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
EciI GGCGGA 2 cut(s) 94, 108
Eco47I GGWCC 2 cut(s) 263, 295
Eco72I CACGTG 1 cut(s) 638
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 3 cut(s) 123, 141, 291
EgeI GGCGCC 1 cut(s) 382
EheI GGCGCC 1 cut(s) 382
Esp3I CGTCTC 1 cut(s) 22
FaiI YATR 1 cut(s) 69
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FauI CCCGC 1 cut(s) 92
FbaI TGATCA 1 cut(s) 330
Fnu4HI GCNGC 1 cut(s) 319
Fsp4HI GCNGC 1 cut(s) 319
FspBI CTAG 2 cut(s) 285, 399
GlaI GCGC 2 cut(s) 6, 382
GluI GCNGC 1 cut(s) 319
GsaI CCCAGC 1 cut(s) 176
HaeII RGCGCY 2 cut(s) 8, 384
HaeIII GGCC 3 cut(s) 218, 291, 452
HapII CCGG 2 cut(s) 108, 303
HhaI GCGC 2 cut(s) 7, 383
Hin1I GRCGYC 1 cut(s) 381
Hin6I GCGC 2 cut(s) 5, 381
HinP1I GCGC 2 cut(s) 5, 381
HinfI GANTC 4 cut(s) 136, 179, 469, 613
HpaII CCGG 2 cut(s) 108, 303
HphI GGTGA 4 cut(s) 68, 292, 292, 622
Hpy166II GTNNAC 1 cut(s) 439
Hpy188I TCNGA 3 cut(s) 474, 567, 618
Hpy188III TCNNGA 1 cut(s) 153
Hpy8I GTNNAC 1 cut(s) 439
Hpy99I CGWCG 1 cut(s) 355
HpyAV CCTTC 2 cut(s) 90, 485
HpyCH4III ACNGT 1 cut(s) 272
HpyCH4IV ACGT 3 cut(s) 15, 350, 637
HpyCH4V TGCA 5 cut(s) 242, 497, 545, 594, 654
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 4 cut(s) 428, 473, 487, 566
HpySE526I ACGT 3 cut(s) 15, 350, 637
Hsp92I GRCGYC 1 cut(s) 381
HspAI GCGC 2 cut(s) 5, 381
KasI GGCGCC 1 cut(s) 380
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 330
Kzo9I GATC 3 cut(s) 330, 424, 574
LguI GCTCTTC 1 cut(s) 34
LweI GCATC 1 cut(s) 400
MaeI CTAG 2 cut(s) 285, 399
MaeII ACGT 3 cut(s) 15, 350, 637
MaeIII GTNAC 2 cut(s) 280, 457
MalI GATC 3 cut(s) 332, 426, 576
MboI GATC 3 cut(s) 330, 424, 574
MboII GAAGA 2 cut(s) 21, 584
MflI RGATCY 1 cut(s) 424
MlsI TGGCCA 1 cut(s) 452
MluCI AATT 2 cut(s) 370, 655
MluNI TGGCCA 1 cut(s) 452
Mly113I GGCGCC 1 cut(s) 381
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 641
Mox20I TGGCCA 1 cut(s) 452
MroNI GCCGGC 1 cut(s) 107
MscI TGGCCA 1 cut(s) 452
MseI TTAA 3 cut(s) 252, 419, 658
MslI CAYNNNNRTG 1 cut(s) 557
Msp20I TGGCCA 1 cut(s) 452
MspI CCGG 2 cut(s) 108, 303
MspR9I CCNGG 3 cut(s) 125, 143, 293
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 3 cut(s) 125, 143, 293
MwoI GCNNNNNNNGC 1 cut(s) 239
NaeI GCCGGC 1 cut(s) 109
NarI GGCGCC 1 cut(s) 381
NdeII GATC 3 cut(s) 330, 424, 574
NgoMIV GCCGGC 1 cut(s) 107
NheI GCTAGC 1 cut(s) 398
NlaIV GGNNCC 3 cut(s) 264, 290, 382
NmeAIII GCCGAG 1 cut(s) 194
NmuCI GTSAC 2 cut(s) 280, 457
OliI CACNNNNGTG 1 cut(s) 557
PciSI GCTCTTC 1 cut(s) 34
PctI GAATGC 1 cut(s) 242
PdiI GCCGGC 1 cut(s) 109
PfeI GAWTC 3 cut(s) 136, 469, 613
Pfl23II CGTACG 1 cut(s) 267
PkrI GCNGC 1 cut(s) 320
PleI GAGTC 1 cut(s) 187
PluTI GGCGCC 1 cut(s) 384
PmaCI CACGTG 1 cut(s) 638
PmlI CACGTG 1 cut(s) 638
PpsI GAGTC 1 cut(s) 187
Ppu21I YACGTR 1 cut(s) 638
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
Psp6I CCWGG 3 cut(s) 123, 141, 291
PspCI CACGTG 1 cut(s) 638
PspFI CCCAGC 1 cut(s) 172
PspGI CCWGG 3 cut(s) 123, 141, 291
PspLI CGTACG 1 cut(s) 267
PspN4I GGNNCC 3 cut(s) 264, 290, 382
PspPI GGNCC 3 cut(s) 263, 289, 295
PspPPI RGGWCCY 1 cut(s) 295
PsuI RGATCY 1 cut(s) 424
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
RseI CAYNNNNRTG 1 cut(s) 557
SapI GCTCTTC 1 cut(s) 34
SaqAI TTAA 3 cut(s) 252, 419, 658
SatI GCNGC 1 cut(s) 319
Sau3AI GATC 3 cut(s) 330, 424, 574
Sau96I GGNCC 3 cut(s) 263, 289, 295
SchI GAGTC 1 cut(s) 188
ScrFI CCNGG 3 cut(s) 125, 143, 293
SfaNI GCATC 1 cut(s) 400
SfoI GGCGCC 1 cut(s) 382
SinI GGWCC 2 cut(s) 263, 295
SmiMI CAYNNNNRTG 1 cut(s) 557
Sse9I AATT 2 cut(s) 370, 655
SsiI CCGC 5 cut(s) 85, 105, 119, 275, 318
SspDI GGCGCC 1 cut(s) 380
SspI AATATT 1 cut(s) 535
SspMI CTAG 2 cut(s) 285, 399
StyD4I CCNGG 3 cut(s) 123, 141, 291
TaaI ACNGT 1 cut(s) 272
TaiI ACGT 3 cut(s) 18, 353, 640
TaqI TCGA 1 cut(s) 353
TasI AATT 2 cut(s) 370, 655
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 3 cut(s) 136, 469, 613
Tru1I TTAA 3 cut(s) 252, 419, 658
Tru9I TTAA 3 cut(s) 252, 419, 658
TseFI GTSAC 2 cut(s) 280, 457
Tsp45I GTSAC 2 cut(s) 280, 457
TspGWI ACGGA 2 cut(s) 172, 255
VpaK11BI GGWCC 2 cut(s) 263, 295
XapI RAATTY 1 cut(s) 370
XspI CTAG 2 cut(s) 285, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.