Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0008710

Physical Location & Seq

Genomic Coordinates Forward (+)

22943975 .. 22947742

Gene Structure 3768 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGTGGGAGTAGCGTACGACTGTCTGGCGAACCCTCTCGGAGCGGTCCGGTCCACTTTCGAGAAAGCGATTGCGTCGGGATCCGACCCAGCCTCCTTTGATGGCCGGGACTGGGGAGCGGTCGATCTCTTCCGCAACTTCCTCTACGATGACGGCGGCCTCTCTCAGGTACCAATTTTGAATTCTGCAAGCATAAGATGGGTTCAGCCCAACACACTCGTTCGATTTCGGGGAATGATACAAGACATGTTGGGAAATGAATTCTATGTCGGTGCTTATAAGGATGGCTCTGTTTGGAGGACCAACAAGTTCACGGATGTTCCTCAATTTCCGGTGGATTTCACGCCGGATATGCGGTTATGGGAACGCCGAATGCTCTACTGCACCCCGGTGCCGGGACAGAACTCATGGGCTGAATCTTCTGACGCGGTGATGTTTTCGAGTATGGAATCAGCAACCCAACAAAGAGAGAAGCGCCCGAGAGTGGATGTTGAAGCTACTGATAACATGGATGTCTCAGATAATGGGATTGAAGGTTCTCCTTCTACCAAAAAGCTCAAGGAAAACGGACATTCTTCTCAGCAGTCAATTACTGAGGGCACCAGCTCTAGTATGGTTACAGTGCCTGATGTCAACAGTGATTCGCTTCCTTGTCTTATTAAGATGTATGATTGTTTAGAATCTGACTCAAAACTAAACGAGGTTTTTGAGTTTGTTGGAGTCTTCACTTTCGATTCAGAGTTTAAAGAGGACAAAGATGAATCAGATGATTTCACGAATGGTTTTAGTGAAGATGTGTTGGTTCACTTGCCCCCAAATAAGGTACCACGTCTCCACTGTTTTATTCACAGGAAACTTGCAGTTCACGACTTCCTTCTGCGTTCCCCTACAATCGAGCCAAAGCCGAACTTGGTGAAAGAGATCAGGGAAGCTCTCTTGAGGCATCTTACAGCTGTTCTTGGCAATGATGGTATAGCTGCTAATTTCGTGCTGTTGCATCTCTTATCCATGGTGTATTCAAGAGTTGGAACTGTTGCTGTGGGGAAGCTTTCACTAAATCTTACTTGTTTAAGCAGAGAAAGTGCGCCTGTGTTTGGCACCCAGCTTGGCCTTGCTCTCAAGAATCTTTTACCTTTCACACAATGCATATCCCTAACAGTGGATTATCTCAACACTGCTTCTCTTGCGCCAAGAAAGGATTATGAGACAAGCAGGCTGATAACCGGAGCCCTGCAGCTGGCTGAGGGTTCACACGTGATATTTGATGAGACCCGGCTGGAAGCTGGCATTCTTAACTCTGTCGGGGTTGAAAATGCAAGATTGCTTAAAACTTTAATAGAGTTGCAAAAGGTGGAATATGATTTCAAGTTCTATAAACTGGACATGCCAGCAGACATCCAAATGCTTGTTCTTTCAGAAGGCAAATCAAACATTTTGCCAGCTGATGTTGTGTTACCTTTCCACCCTTCTTCAGTGGCTTCCGCTGAAGTCACCGCAGAAGCACTAGAGGCGTGGAGATGGTACTTGGCTACTCTTAGATCATTGCCACATTCTATTGACTCAGAATTACAGAAGGTGATAGAAAATGACTTGGTTGCAGCAAGGCAGGAAGATAGGACACTGGGAACCCAAGATTTCAGCAGATTGCTGACAATGGGTCGATTGATGTCAATGAGTTTTGGTGAAACCTCCCTATCATTGGAACACTGGCAGATGGTCAAGGAACTCGAGAGGCTACGAAGAGAGAGGCTCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0000819 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006281 GO:0006301 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009526 GO:0009536 GO:0009941 GO:0009987 GO:0016043 GO:0022402 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0042555 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0098813 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MCM_bind

Protein Analysis

586

Amino Acids

65.47

Weight (kDa)

5.11

Isoelectric Point (pI)

45.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCM_bind	PF09739	44 - 584	5.5e-189	Mini-chromosome maintenance replisome factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008710)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40550
fragaria_vesca	FvH4_7g25850 FvH4_7g25850 FvH4_7g25850 FvH4_7g25850 FvH4_7g25850
malus_domestica	MD01G1166300.v1.1 MD01G1183300.v1.1 MD07G1233000.v1.1
prunus_persica	Prupe.2G263500_v2.0.a1 Prupe.2G263500_v2.0.a1
pyrus_communis	pycom01g17990 pycom07g21080
rosa_chinensis	RchiOBHm_Chr1g0372121
rosa_laevigata	RLG00000026921
rosa_multiflora	Rmu_sc0001755.1_g000024
rosa_roxburghii	Rroxscaffold_4G00285230
rosa_rugosa	Rorug01G0369900
rosa_samantha	Rh1AG378300 Rh1BG341600 Rh1CG355000 Rh1DG372700
rosa_wichuraiana	Rw1G033280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	279
Acc65I	GGTACC	2 cut(s)	169, 823
AccB1I	GGYRCC	5 cut(s)	169, 391, 599, 823, 1097
AccBSI	CCGCTC	2 cut(s)	44, 119
AccII	CGCG	1 cut(s)	428
AciI	CCGC	8 cut(s)	44, 119, 133, 156, 355, 428, 1482, 1494
AclWI	GGATC	2 cut(s)	75, 88
AcoI	YGGCCR	1 cut(s)	103
AcsI	RAATTY	2 cut(s)	181, 260
AcuI	CTGAAG	2 cut(s)	1455, 1506
AcvI	CACGTG	1 cut(s)	1255
AfaI	GTAC	4 cut(s)	17, 171, 825, 1523
AflIII	ACRYGT	2 cut(s)	246, 1252
AgsI	TTSAA	6 cut(s)	181, 494, 533, 1020, 1310, 1366
AhdI	GACNNNNNGTC	1 cut(s)	1656
AjiI	CACGTC	1 cut(s)	830
AjuI	GAANNNNNNNTTGG	4 cut(s)	166, 198, 1392, 1424
AleI	CACNNNNGTG	1 cut(s)	389
AloI	GAACNNNNNNTCC	2 cut(s)	520, 552
Alw26I	GTCTC	4 cut(s)	520, 836, 1199, 1262
AlwI	GGATC	2 cut(s)	75, 88
AlwNI	CAGNNNCTG	1 cut(s)	626
Ama87I	CYCGRG	2 cut(s)	478, 1727
AoxI	GGCC	3 cut(s)	103, 157, 1108
ApeKI	GCWGC	3 cut(s)	977, 1234, 1598
ApoI	RAATTY	2 cut(s)	181, 260
Asp718I	GGTACC	2 cut(s)	169, 823
AspLEI	GCGC	3 cut(s)	477, 1087, 1189
AspS9I	GGNCC	3 cut(s)	46, 51, 300
AsuC2I	CCSGG	4 cut(s)	107, 389, 396, 1273
AsuHPI	GGTGA	5 cut(s)	442, 925, 1483, 1588, 1694
AvaI	CYCGRG	2 cut(s)	478, 1727
AvaII	GGWCC	3 cut(s)	46, 51, 300
BaeGI	GKGCMC	1 cut(s)	602
BamHI	GGATCC	1 cut(s)	80
BanI	GGYRCC	5 cut(s)	169, 391, 599, 823, 1097
BanII	GRGCYC	1 cut(s)	1231
BbrPI	CACGTG	1 cut(s)	1255
BbsI	GAAGAC	1 cut(s)	715
BbvCI	CCTCAGC	1 cut(s)	1242
BbvI	GCAGC	3 cut(s)	964, 1246, 1610
BccI	CCATC	6 cut(s)	95, 192, 278, 962, 1512, 1708
BceAI	ACGGC	1 cut(s)	169
BcnI	CCSGG	4 cut(s)	107, 389, 396, 1273
BcoDI	GTCTC	4 cut(s)	520, 836, 1199, 1262
BfaI	CTAG	2 cut(s)	609, 1505
BfmI	CTRYAG	1 cut(s)	1232
BfoI	RGCGCY	1 cut(s)	478
BisI	GCNGC	4 cut(s)	157, 978, 1235, 1599
BlsI	GCNGC	4 cut(s)	158, 979, 1236, 1600
Bme1390I	CCNGG	4 cut(s)	107, 389, 396, 1273
Bme18I	GGWCC	3 cut(s)	46, 51, 300
BmeRI	GACNNNNNGTC	1 cut(s)	1656
BmeT110I	CYCGRG	2 cut(s)	478, 1727
BmgBI	CACGTC	1 cut(s)	830
BmgT120I	GGNCC	3 cut(s)	46, 51, 300
BmiI	GGNNCC	8 cut(s)	82, 171, 393, 601, 825, 1099, 1228, 1627
BmrFI	CCNGG	4 cut(s)	107, 389, 396, 1273
BmrI	ACTGGG	2 cut(s)	121, 1631
BmsI	GCATC	2 cut(s)	952, 1006
BmuI	ACTGGG	2 cut(s)	121, 1631
BpiI	GAAGAC	1 cut(s)	715
BplI	GAGNNNNNCTC	1 cut(s)	1734
Bpu10I	CCTNAGC	1 cut(s)	1242
BpuEI	CTTGAG	4 cut(s)	542, 958, 1103, 1736
BpuMI	CCSGG	4 cut(s)	107, 389, 396, 1273
BsaAI	YACGTR	1 cut(s)	1255
BsaI	GGTCTC	1 cut(s)	1262
BsaJI	CCNNGG	2 cut(s)	387, 1008
BsaWI	WCCGGW	3 cut(s)	48, 331, 1223
BsaXI	ACNNNNNCTCC	4 cut(s)	77, 107, 816, 846
Bse1I	ACTGG	4 cut(s)	116, 1383, 1626, 1712
Bse3DI	GCAATG	2 cut(s)	970, 1541
BseDI	CCNNGG	2 cut(s)	387, 1008
BseGI	GGATG	5 cut(s)	289, 322, 493, 517, 1395
BseMI	GCAATG	2 cut(s)	970, 1541
BseMII	CTCAG	6 cut(s)	179, 531, 585, 593, 1233, 1575
BseNI	ACTGG	4 cut(s)	116, 1383, 1626, 1712
BseSI	GKGCMC	1 cut(s)	602
BseXI	GCAGC	3 cut(s)	964, 1246, 1610
BseYI	CCCAGC	2 cut(s)	88, 1101
BsgI	GTGCAG	1 cut(s)	367
Bsh1236I	CGCG	1 cut(s)	428
Bsh1285I	CGRYCG	1 cut(s)	123
BshFI	GGCC	3 cut(s)	105, 159, 1110
BshNI	GGYRCC	5 cut(s)	169, 391, 599, 823, 1097
BsiEI	CGRYCG	1 cut(s)	123
BsiHKCI	CYCGRG	2 cut(s)	478, 1727
BsiSI	CCGG	8 cut(s)	49, 106, 332, 347, 389, 395, 1224, 1273
BsiWI	CGTACG	1 cut(s)	15
BslFI	GGGAC	2 cut(s)	122, 411
BsmAI	GTCTC	4 cut(s)	520, 836, 1199, 1262
BsmBI	CGTCTC	1 cut(s)	836
BsmFI	GGGAC	2 cut(s)	122, 411
BsmI	GAATGC	2 cut(s)	378, 1287
BsnI	GGCC	3 cut(s)	105, 159, 1110
Bso31I	GGTCTC	1 cut(s)	1262
BsoBI	CYCGRG	2 cut(s)	478, 1727
Bsp1286I	GDGCHC	2 cut(s)	602, 1231
Bsp143I	GATC	4 cut(s)	80, 124, 921, 1538
Bsp19I	CCATGG	1 cut(s)	1008
BspACI	CCGC	8 cut(s)	44, 119, 133, 156, 355, 428, 1482, 1494
BspANI	GGCC	3 cut(s)	105, 159, 1110
BspCNI	CTCAG	6 cut(s)	178, 530, 586, 592, 1234, 1574
BspFNI	CGCG	1 cut(s)	428
BspLI	GGNNCC	8 cut(s)	82, 171, 393, 601, 825, 1099, 1228, 1627
BspMAI	CTGCAG	1 cut(s)	1236
BspPI	GGATC	2 cut(s)	75, 88
BspT107I	GGYRCC	5 cut(s)	169, 391, 599, 823, 1097
BspTNI	GGTCTC	1 cut(s)	1262
BsrBI	CCGCTC	2 cut(s)	44, 119
BsrDI	GCAATG	2 cut(s)	970, 1541
BsrI	ACTGG	4 cut(s)	116, 1383, 1626, 1712
BssECI	CCNNGG	2 cut(s)	387, 1008
BssMI	GATC	4 cut(s)	80, 124, 921, 1538
BssT1I	CCWWGG	1 cut(s)	1008
Bst4CI	ACNGT	6 cut(s)	23, 622, 638, 839, 1033, 1159
Bst6I	CTCTTC	2 cut(s)	134, 1735
BstBAI	YACGTR	1 cut(s)	1255
BstC8I	GCNNGC	6 cut(s)	190, 1214, 1239, 1285, 1389, 1440
BstDEI	CTNAG	7 cut(s)	165, 517, 579, 594, 1242, 1535, 1561
BstDSI	CCRYGG	1 cut(s)	1008
BstENI	CCTNNNNNAGG	1 cut(s)	164
BstF5I	GGATG	5 cut(s)	289, 322, 493, 517, 1395
BstFNI	CGCG	1 cut(s)	428
BstH2I	RGCGCY	1 cut(s)	478
BstHHI	GCGC	3 cut(s)	477, 1087, 1189
BstKTI	GATC	4 cut(s)	83, 127, 924, 1541
BstMAI	GTCTC	4 cut(s)	520, 836, 1199, 1262
BstMBI	GATC	4 cut(s)	80, 124, 921, 1538
BstMCI	CGRYCG	1 cut(s)	123
BstMWI	GCNNNNNNNGC	3 cut(s)	352, 1184, 1508
BstNSI	RCATGY	2 cut(s)	250, 1387
BstSCI	CCNGG	4 cut(s)	105, 387, 394, 1271
BstSFI	CTRYAG	1 cut(s)	1232
BstSLI	GKGCMC	1 cut(s)	602
BstUI	CGCG	1 cut(s)	428
BstV1I	GCAGC	3 cut(s)	964, 1246, 1610
BstV2I	GAAGAC	1 cut(s)	715
BstX2I	RGATCY	1 cut(s)	80
BstYI	RGATCY	1 cut(s)	80
BsuRI	GGCC	3 cut(s)	105, 159, 1110
BtgI	CCRYGG	1 cut(s)	1008
BtrI	CACGTC	1 cut(s)	830
BtsCI	GGATG	5 cut(s)	289, 322, 493, 517, 1395
BtsI	GCAGTG	1 cut(s)	1173
BtsIMutI	CAGTG	8 cut(s)	627, 643, 835, 1164, 1173, 1479, 1619, 1705
Cac8I	GCNNGC	6 cut(s)	190, 1214, 1239, 1285, 1389, 1440
CaiI	CAGNNNCTG	1 cut(s)	626
CfoI	GCGC	3 cut(s)	477, 1087, 1189
Cfr13I	GGNCC	3 cut(s)	46, 51, 300
CpoI	CGGWCCG	1 cut(s)	46
CseI	GACGC	2 cut(s)	63, 434
Csp6I	GTAC	4 cut(s)	16, 170, 824, 1522
CspI	CGGWCCG	1 cut(s)	46
CviAII	CATG	5 cut(s)	247, 408, 508, 1009, 1384
CviQI	GTAC	4 cut(s)	16, 170, 824, 1522
DdeI	CTNAG	7 cut(s)	165, 517, 579, 594, 1242, 1535, 1561
DpnI	GATC	4 cut(s)	82, 126, 923, 1540
DpnII	GATC	4 cut(s)	80, 124, 921, 1538
DraI	TTTAAA	1 cut(s)	745
DriI	GACNNNNNGTC	1 cut(s)	1656
EaeI	YGGCCR	1 cut(s)	103
Eam1104I	CTCTTC	2 cut(s)	134, 1735
Eam1105I	GACNNNNNGTC	1 cut(s)	1656
EarI	CTCTTC	2 cut(s)	134, 1735
Eco130I	CCWWGG	1 cut(s)	1008
Eco24I	GRGCYC	1 cut(s)	1231
Eco31I	GGTCTC	1 cut(s)	1262
Eco47I	GGWCC	3 cut(s)	46, 51, 300
Eco57I	CTGAAG	2 cut(s)	1455, 1506
Eco72I	CACGTG	1 cut(s)	1255
Eco88I	CYCGRG	2 cut(s)	478, 1727
EcoNI	CCTNNNNNAGG	1 cut(s)	164
EcoRI	GAATTC	2 cut(s)	181, 260
EcoT14I	CCWWGG	1 cut(s)	1008
EcoT22I	ATGCAT	1 cut(s)	1148
EcoT38I	GRGCYC	1 cut(s)	1231
ErhI	CCWWGG	1 cut(s)	1008
Esp3I	CGTCTC	1 cut(s)	836
FaeI	CATG	5 cut(s)	250, 411, 511, 1012, 1387
FalI	AAGNNNNNCTT	2 cut(s)	893, 925
FaqI	GGGAC	2 cut(s)	122, 411
FatI	CATG	5 cut(s)	246, 407, 507, 1008, 1383
Fnu4HI	GCNGC	4 cut(s)	157, 978, 1235, 1599
FokI	GGATG	5 cut(s)	296, 329, 500, 524, 1382
FriOI	GRGCYC	1 cut(s)	1231
Fsp4HI	GCNGC	4 cut(s)	157, 978, 1235, 1599
FspBI	CTAG	2 cut(s)	609, 1505
GlaI	GCGC	3 cut(s)	476, 1086, 1188
GluI	GCNGC	4 cut(s)	157, 978, 1235, 1599
GsaI	CCCAGC	2 cut(s)	92, 1105
HaeII	RGCGCY	1 cut(s)	478
HaeIII	GGCC	3 cut(s)	105, 159, 1110
HapII	CCGG	8 cut(s)	49, 106, 332, 347, 389, 395, 1224, 1273
HgaI	GACGC	2 cut(s)	63, 434
HhaI	GCGC	3 cut(s)	477, 1087, 1189
Hin1II	CATG	5 cut(s)	250, 411, 511, 1012, 1387
Hin6I	GCGC	3 cut(s)	475, 1085, 1187
HinP1I	GCGC	3 cut(s)	475, 1085, 1187
HincII	GTYRAC	1 cut(s)	634
HindII	GTYRAC	1 cut(s)	634
HindIII	AAGCTT	1 cut(s)	1046
HpaII	CCGG	8 cut(s)	49, 106, 332, 347, 389, 395, 1224, 1273
HphI	GGTGA	5 cut(s)	442, 925, 1483, 1588, 1694
Hpy166II	GTNNAC	6 cut(s)	54, 312, 634, 805, 865, 1250
Hpy188I	TCNGA	9 cut(s)	41, 85, 424, 520, 685, 739, 766, 1417, 1564
Hpy188III	TCNNGA	8 cut(s)	61, 78, 775, 866, 937, 1020, 1120, 1729
Hpy8I	GTNNAC	6 cut(s)	54, 312, 634, 805, 865, 1250
Hpy99I	CGWCG	1 cut(s)	79
HpyAV	CCTTC	6 cut(s)	527, 552, 884, 1412, 1476, 1567
HpyCH4III	ACNGT	6 cut(s)	23, 622, 638, 839, 1033, 1159
HpyCH4IV	ACGT	2 cut(s)	829, 1254
HpyCH4V	TGCA	9 cut(s)	188, 384, 860, 997, 1146, 1234, 1316, 1345, 1598
HpyF10VI	GCNNNNNNNGC	3 cut(s)	352, 1184, 1508
HpyF3I	CTNAG	7 cut(s)	165, 517, 579, 594, 1242, 1535, 1561
HpySE526I	ACGT	2 cut(s)	829, 1254
Hsp92II	CATG	5 cut(s)	250, 411, 511, 1012, 1387
HspAI	GCGC	3 cut(s)	475, 1085, 1187
KpnI	GGTACC	2 cut(s)	173, 827
Kzo9I	GATC	4 cut(s)	80, 124, 921, 1538
LmnI	GCTCC	3 cut(s)	41, 116, 1226
Lsp1109I	GCAGC	3 cut(s)	964, 1246, 1610
LweI	GCATC	2 cut(s)	952, 1006
MaeI	CTAG	2 cut(s)	609, 1505
MaeII	ACGT	2 cut(s)	829, 1254
MaeIII	GTNAC	3 cut(s)	616, 1452, 1489
MalI	GATC	4 cut(s)	82, 126, 923, 1540
MbiI	CCGCTC	2 cut(s)	44, 119
MboI	GATC	4 cut(s)	80, 124, 921, 1538
MboII	GAAGA	8 cut(s)	121, 411, 567, 715, 803, 1461, 1622, 1752
MflI	RGATCY	1 cut(s)	80
MhlI	GDGCHC	2 cut(s)	602, 1231
MluCI	AATT	7 cut(s)	174, 181, 260, 326, 588, 982, 1565
MlyI	GAGTC	3 cut(s)	680, 729, 1553
MmeI	TCCRAC	3 cut(s)	108, 697, 1006
Mph1103I	ATGCAT	1 cut(s)	1148
MseI	TTAA	6 cut(s)	660, 744, 1070, 1293, 1326, 1334
MslI	CAYNNNNRTG	2 cut(s)	389, 1400
MspA1I	CMGCKG	4 cut(s)	953, 1237, 1442, 1484
MspI	CCGG	8 cut(s)	49, 106, 332, 347, 389, 395, 1224, 1273
MspR9I	CCNGG	4 cut(s)	107, 389, 396, 1273
Mva1269I	GAATGC	2 cut(s)	378, 1287
MvnI	CGCG	1 cut(s)	428
MwoI	GCNNNNNNNGC	3 cut(s)	352, 1184, 1508
NciI	CCSGG	4 cut(s)	107, 389, 396, 1273
NcoI	CCATGG	1 cut(s)	1008
NdeII	GATC	4 cut(s)	80, 124, 921, 1538
NlaIII	CATG	5 cut(s)	250, 411, 511, 1012, 1387
NlaIV	GGNNCC	8 cut(s)	82, 171, 393, 601, 825, 1099, 1228, 1627
NmuCI	GTSAC	1 cut(s)	1489
NsiI	ATGCAT	1 cut(s)	1148
NspI	RCATGY	2 cut(s)	250, 1387
OliI	CACNNNNGTG	1 cut(s)	389
PaeR7I	CTCGAG	1 cut(s)	1727
PciI	ACATGT	1 cut(s)	246
PctI	GAATGC	2 cut(s)	378, 1287
PfeI	GAWTC	7 cut(s)	416, 449, 641, 680, 734, 761, 1123
Pfl23II	CGTACG	1 cut(s)	15
PkrI	GCNGC	4 cut(s)	158, 979, 1236, 1600
PleI	GAGTC	3 cut(s)	680, 728, 1553
PmaCI	CACGTG	1 cut(s)	1255
PmlI	CACGTG	1 cut(s)	1255
PpsI	GAGTC	3 cut(s)	680, 728, 1553
Ppu21I	YACGTR	1 cut(s)	1255
PscI	ACATGT	1 cut(s)	246
PsiI	TTATAA	1 cut(s)	279
PspCI	CACGTG	1 cut(s)	1255
PspFI	CCCAGC	2 cut(s)	88, 1101
PspLI	CGTACG	1 cut(s)	15
PspN4I	GGNNCC	8 cut(s)	82, 171, 393, 601, 825, 1099, 1228, 1627
PspPI	GGNCC	3 cut(s)	46, 51, 300
PstI	CTGCAG	1 cut(s)	1236
PstNI	CAGNNNCTG	1 cut(s)	626
PsuI	RGATCY	1 cut(s)	80
PvuII	CAGCTG	3 cut(s)	953, 1237, 1442
RsaI	GTAC	4 cut(s)	17, 171, 825, 1523
RsaNI	GTAC	4 cut(s)	16, 170, 824, 1522
RseI	CAYNNNNRTG	2 cut(s)	389, 1400
Rsr2I	CGGWCCG	1 cut(s)	46
RsrII	CGGWCCG	1 cut(s)	46
SaqAI	TTAA	6 cut(s)	660, 744, 1070, 1293, 1326, 1334
SatI	GCNGC	4 cut(s)	157, 978, 1235, 1599
Sau3AI	GATC	4 cut(s)	80, 124, 921, 1538
Sau96I	GGNCC	3 cut(s)	46, 51, 300
SchI	GAGTC	3 cut(s)	680, 729, 1553
ScrFI	CCNGG	4 cut(s)	107, 389, 396, 1273
SduI	GDGCHC	2 cut(s)	602, 1231
SfaNI	GCATC	2 cut(s)	952, 1006
SfcI	CTRYAG	1 cut(s)	1232
Sfr274I	CTCGAG	1 cut(s)	1727
SinI	GGWCC	3 cut(s)	46, 51, 300
SlaI	CTCGAG	1 cut(s)	1727
SmiMI	CAYNNNNRTG	2 cut(s)	389, 1400
SmlI	CTYRAG	5 cut(s)	557, 937, 1118, 1727, 1751
SmoI	CTYRAG	5 cut(s)	557, 937, 1118, 1727, 1751
Sse9I	AATT	7 cut(s)	174, 181, 260, 326, 588, 982, 1565
SsiI	CCGC	8 cut(s)	44, 119, 133, 156, 355, 428, 1482, 1494
SspMI	CTAG	2 cut(s)	609, 1505
StyD4I	CCNGG	4 cut(s)	105, 387, 394, 1271
StyI	CCWWGG	1 cut(s)	1008
TaaI	ACNGT	6 cut(s)	23, 622, 638, 839, 1033, 1159
TaiI	ACGT	2 cut(s)	832, 1257
TaqI	TCGA	8 cut(s)	60, 123, 223, 440, 732, 894, 1660, 1728
TasI	AATT	7 cut(s)	174, 181, 260, 326, 588, 982, 1565
TauI	GCSGC	1 cut(s)	159
TfiI	GAWTC	7 cut(s)	416, 449, 641, 680, 734, 761, 1123
Tru1I	TTAA	6 cut(s)	660, 744, 1070, 1293, 1326, 1334
Tru9I	TTAA	6 cut(s)	660, 744, 1070, 1293, 1326, 1334
TscAI	CASTG	8 cut(s)	627, 643, 842, 1164, 1180, 1479, 1626, 1712
TseFI	GTSAC	1 cut(s)	1489
TseI	GCWGC	3 cut(s)	977, 1234, 1598
Tsp45I	GTSAC	1 cut(s)	1489
TspDTI	ATGAA	2 cut(s)	273, 774
TspGWI	ACGGA	2 cut(s)	329, 582
TspRI	CASTG	8 cut(s)	627, 643, 842, 1164, 1180, 1479, 1626, 1712
VpaK11BI	GGWCC	3 cut(s)	46, 51, 300
XagI	CCTNNNNNAGG	1 cut(s)	164
XapI	RAATTY	2 cut(s)	181, 260
XceI	RCATGY	2 cut(s)	250, 1387
XcmI	CCANNNNNNNNNTGG	1 cut(s)	610
XhoI	CTCGAG	1 cut(s)	1727
XspI	CTAG	2 cut(s)	609, 1505
Zsp2I	ATGCAT	1 cut(s)	1148

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.