pycom07g21820

Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006090
Physical Location & Seq
Forward (+)
23359853 .. 23360731
879 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGGAGGCCCTGATTGCCTCGTACGGAGACTCGTCGTCGGACTCAGGCTCCGAGTCGCTGGCTCCACCTCCGCCACCACCTTCAGAGCTCAAAAACTCTCAAGAACCGACCACTGCGCTGCCTCCACCTCCTCTGTCACTTCTCGACAGCCCCAATTCCTTCGGATTTCTGGACTTCTCCCCAAGCGCCCAGCCGAGCAGAGTTCGGAACTTTGCTCACGTCGAAGGGAACTACGCAGTACATGTATACATCCCAGTTCATATACCACCAGCACCGAGGAAAGAGATGGCCTTGTTTTTGAACAAGCTAGCTTCTCTGGTGCCTGGTCTCCATGCCGTTGACGTTGACGTCCCGCTTGACGTTCTGCGTAAGGACGAGCGTAAGCTTGAACAGGTTGCTTTAGGCAGAGAGTTCCATATAAGTCTCGGAAGAACTGTTCCGATTCGGGTGCACCAGATTGATTCCTTGGTGACAATGCTGCGGCAGAAGCTTCAGATTCAGAGGCGGTATTGGATTGATTTTAGCAAGTGGGAGGTTTTTGTTAACGATGATCGATCCTATAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HVSL

Protein Analysis

189

Amino Acids

21.02

Weight (kDa)

6.06

Isoelectric Point (pI)

59.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HVSL PF09749 41 - 186 1.8e-27 Uncharacterised conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 347
AatII GACGTC 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 319
AccI GTMKAC 1 cut(s) 246
AciI CCGC 4 cut(s) 71, 353, 481, 505
AclWI GGATC 1 cut(s) 549
AcuI CTGAAG 2 cut(s) 66, 476
AcyI GRCGYC 1 cut(s) 348
AfaI GTAC 2 cut(s) 23, 240
AflIII ACRYGT 1 cut(s) 241
AgsI TTSAA 2 cut(s) 301, 389
AhdI GACNNNNNGTC 1 cut(s) 34
AjiI CACGTC 1 cut(s) 220
AjnI CCWGG 1 cut(s) 322
AloI GAACNNNNNNTCC 2 cut(s) 420, 452
AluBI AGCT 6 cut(s) 88, 307, 311, 385, 490, 564
AluI AGCT 6 cut(s) 88, 307, 311, 385, 490, 564
Alw21I GWGCWC 2 cut(s) 90, 453
Alw26I GTCTC 3 cut(s) 21, 332, 428
Alw44I GTGCAC 1 cut(s) 449
AlwI GGATC 1 cut(s) 549
AoxI GGCC 2 cut(s) 6, 288
ApaLI GTGCAC 1 cut(s) 449
ApeKI GCWGC 2 cut(s) 118, 478
AspLEI GCGC 2 cut(s) 118, 188
AspS9I GGNCC 1 cut(s) 7
AsuHPI GGTGA 1 cut(s) 481
AsuNHI GCTAGC 1 cut(s) 307
BaeGI GKGCMC 1 cut(s) 453
BanI GGYRCC 1 cut(s) 319
BanII GRGCYC 1 cut(s) 90
Bbv12I GWGCWC 2 cut(s) 90, 453
BbvI GCAGC 2 cut(s) 105, 465
BccI CCATC 1 cut(s) 280
BceAI ACGGC 1 cut(s) 320
BcgI CGANNNNNNTGC 2 cut(s) 430, 464
BciT130I CCWGG 1 cut(s) 324
BcoDI GTCTC 3 cut(s) 21, 332, 428
BfaI CTAG 1 cut(s) 308
BfmI CTRYAG 1 cut(s) 559
BfoI RGCGCY 1 cut(s) 189
BisI GCNGC 3 cut(s) 119, 479, 482
BlsI GCNGC 3 cut(s) 120, 480, 483
Bme1390I CCNGG 1 cut(s) 324
BmeRI GACNNNNNGTC 1 cut(s) 34
BmgBI CACGTC 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 7
BmiI GGNNCC 3 cut(s) 49, 63, 321
BmrFI CCNGG 1 cut(s) 324
BmrI ACTGGG 1 cut(s) 248
BmtI GCTAGC 1 cut(s) 311
BmuI ACTGGG 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 84
Bsa29I ATCGAT 1 cut(s) 553
BsaHI GRCGYC 1 cut(s) 348
BsaI GGTCTC 1 cut(s) 332
BsaJI CCNNGG 2 cut(s) 275, 465
BsaXI ACNNNNNCTCC 2 cut(s) 32, 62
Bse1I ACTGG 1 cut(s) 254
BseBI CCWGG 1 cut(s) 324
BseCI ATCGAT 1 cut(s) 553
BseDI CCNNGG 2 cut(s) 275, 465
BseGI GGATG 1 cut(s) 249
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 1 cut(s) 254
BseRI GAGGAG 1 cut(s) 120
BseSI GKGCMC 1 cut(s) 453
BseXI GCAGC 2 cut(s) 105, 465
BseYI CCCAGC 1 cut(s) 189
BshFI GGCC 2 cut(s) 8, 290
BshNI GGYRCC 1 cut(s) 319
BshVI ATCGAT 1 cut(s) 553
BsiHKAI GWGCWC 2 cut(s) 90, 453
BsiWI CGTACG 1 cut(s) 21
BslFI GGGAC 1 cut(s) 335
BsmAI GTCTC 3 cut(s) 21, 332, 428
BsmFI GGGAC 1 cut(s) 335
BsnI GGCC 2 cut(s) 8, 290
Bso31I GGTCTC 1 cut(s) 332
Bsp1286I GDGCHC 2 cut(s) 90, 453
Bsp143I GATC 2 cut(s) 550, 554
BspACI CCGC 4 cut(s) 71, 353, 481, 505
BspANI GGCC 2 cut(s) 8, 290
BspCNI CTCAG 1 cut(s) 56
BspDI ATCGAT 1 cut(s) 553
BspLI GGNNCC 3 cut(s) 49, 63, 321
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 1 cut(s) 549
BspT107I GGYRCC 1 cut(s) 319
BspTNI GGTCTC 1 cut(s) 332
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 2 cut(s) 275, 465
BssMI GATC 2 cut(s) 550, 554
BssNAI GTATAC 1 cut(s) 247
BssNI GRCGYC 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 465
Bst1107I GTATAC 1 cut(s) 247
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 436
BstACI GRCGYC 1 cut(s) 348
BstC8I GCNNGC 2 cut(s) 60, 309
BstDEI CTNAG 1 cut(s) 43
BstF5I GGATG 1 cut(s) 249
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 2 cut(s) 118, 188
BstKTI GATC 2 cut(s) 553, 557
BstMAI GTCTC 3 cut(s) 21, 332, 428
BstMBI GATC 2 cut(s) 550, 554
BstMWI GCNNNNNNNGC 2 cut(s) 14, 487
BstNI CCWGG 1 cut(s) 324
BstNSI RCATGY 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 322
BstSFI CTRYAG 1 cut(s) 559
BstSLI GKGCMC 1 cut(s) 453
BstV1I GCAGC 2 cut(s) 105, 465
BstZ17I GTATAC 1 cut(s) 247
Bsu15I ATCGAT 1 cut(s) 553
BsuRI GGCC 2 cut(s) 8, 290
BsuTUI ATCGAT 1 cut(s) 553
BtrI CACGTC 1 cut(s) 220
BtsCI GGATG 1 cut(s) 249
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 2 cut(s) 60, 309
CfoI GCGC 2 cut(s) 118, 188
Cfr13I GGNCC 1 cut(s) 7
ClaI ATCGAT 1 cut(s) 553
Csp6I GTAC 2 cut(s) 22, 239
CviAII CATG 2 cut(s) 242, 332
CviQI GTAC 2 cut(s) 22, 239
DdeI CTNAG 1 cut(s) 43
DpnI GATC 2 cut(s) 552, 556
DpnII GATC 2 cut(s) 550, 554
DrdI GACNNNNNNGTC 1 cut(s) 347
DriI GACNNNNNGTC 1 cut(s) 34
DseDI GACNNNNNNGTC 1 cut(s) 347
Eam1105I GACNNNNNGTC 1 cut(s) 34
EciI GGCGGA 1 cut(s) 60
Ecl136II GAGCTC 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 90
Eco31I GGTCTC 1 cut(s) 332
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 2 cut(s) 66, 476
EcoICRI GAGCTC 1 cut(s) 88
EcoO109I RGGNCCY 1 cut(s) 7
EcoRII CCWGG 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 465
EcoT38I GRGCYC 1 cut(s) 90
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 2 cut(s) 245, 335
FaiI YATR 8 cut(s) 243, 247, 261, 263, 333, 417, 419, 561
FaqI GGGAC 1 cut(s) 335
FatI CATG 2 cut(s) 241, 331
FauI CCCGC 1 cut(s) 360
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 3 cut(s) 119, 479, 482
FokI GGATG 1 cut(s) 236
FriOI GRGCYC 1 cut(s) 90
Fsp4HI GCNGC 3 cut(s) 119, 479, 482
FspBI CTAG 1 cut(s) 308
GlaI GCGC 2 cut(s) 117, 187
GluI GCNGC 3 cut(s) 119, 479, 482
GsaI CCCAGC 1 cut(s) 193
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 2 cut(s) 8, 290
HhaI GCGC 2 cut(s) 118, 188
Hin1I GRCGYC 1 cut(s) 348
Hin1II CATG 2 cut(s) 245, 335
Hin6I GCGC 2 cut(s) 116, 186
HinP1I GCGC 2 cut(s) 116, 186
HincII GTYRAC 3 cut(s) 340, 346, 544
HindII GTYRAC 3 cut(s) 340, 346, 544
HindIII AAGCTT 2 cut(s) 383, 488
HinfI GANTC 6 cut(s) 29, 41, 53, 442, 461, 496
HpaI GTTAAC 1 cut(s) 544
HphI GGTGA 1 cut(s) 481
Hpy166II GTNNAC 5 cut(s) 247, 340, 346, 451, 544
Hpy188I TCNGA 9 cut(s) 40, 52, 85, 164, 207, 428, 441, 495, 501
Hpy188III TCNNGA 3 cut(s) 101, 143, 170
Hpy8I GTNNAC 5 cut(s) 247, 340, 346, 451, 544
Hpy99I CGWCG 3 cut(s) 37, 40, 224
HpyAV CCTTC 3 cut(s) 90, 169, 218
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 4 cut(s) 219, 342, 348, 360
HpyCH4V TGCA 1 cut(s) 451
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 487
HpyF3I CTNAG 1 cut(s) 43
HpySE526I ACGT 4 cut(s) 219, 342, 348, 360
Hsp92I GRCGYC 1 cut(s) 348
Hsp92II CATG 2 cut(s) 245, 335
HspAI GCGC 2 cut(s) 116, 186
KspAI GTTAAC 1 cut(s) 544
Kzo9I GATC 2 cut(s) 550, 554
LmnI GCTCC 2 cut(s) 53, 67
Lsp1109I GCAGC 2 cut(s) 105, 465
MaeI CTAG 1 cut(s) 308
MaeII ACGT 4 cut(s) 219, 342, 348, 360
MaeIII GTNAC 2 cut(s) 135, 469
MalI GATC 2 cut(s) 552, 556
MboI GATC 2 cut(s) 550, 554
MboII GAAGA 1 cut(s) 441
MhlI GDGCHC 2 cut(s) 90, 453
MluCI AATT 1 cut(s) 154
MlyI GAGTC 3 cut(s) 23, 35, 62
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 8 cut(s) 28, 78, 132, 138, 141, 270, 495, 526
MseI TTAA 1 cut(s) 543
MspR9I CCNGG 1 cut(s) 324
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 2 cut(s) 14, 487
NdeII GATC 2 cut(s) 550, 554
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 2 cut(s) 245, 335
NlaIV GGNNCC 3 cut(s) 49, 63, 321
NmeAIII GCCGAG 1 cut(s) 219
NmuCI GTSAC 2 cut(s) 135, 469
NspI RCATGY 1 cut(s) 245
PciI ACATGT 1 cut(s) 241
PfeI GAWTC 3 cut(s) 442, 461, 496
Pfl23II CGTACG 1 cut(s) 21
PkrI GCNGC 3 cut(s) 120, 480, 483
PleI GAGTC 3 cut(s) 23, 35, 61
PpsI GAGTC 3 cut(s) 23, 35, 61
PscI ACATGT 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 322
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 1 cut(s) 322
PspLI CGTACG 1 cut(s) 21
PspN4I GGNNCC 3 cut(s) 49, 63, 321
PspPI GGNCC 1 cut(s) 7
RsaI GTAC 2 cut(s) 23, 240
RsaNI GTAC 2 cut(s) 22, 239
SacI GAGCTC 1 cut(s) 90
SaqAI TTAA 1 cut(s) 543
SatI GCNGC 3 cut(s) 119, 479, 482
Sau3AI GATC 2 cut(s) 550, 554
Sau96I GGNCC 1 cut(s) 7
SchI GAGTC 3 cut(s) 23, 35, 62
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 2 cut(s) 90, 453
SfcI CTRYAG 1 cut(s) 559
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
Sse9I AATT 1 cut(s) 154
SsiI CCGC 4 cut(s) 71, 353, 481, 505
SspMI CTAG 1 cut(s) 308
SstI GAGCTC 1 cut(s) 90
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 4 cut(s) 222, 345, 351, 363
TaqI TCGA 3 cut(s) 144, 222, 553
TasI AATT 1 cut(s) 154
TatI WGTACW 1 cut(s) 238
TauI GCSGC 1 cut(s) 484
TfiI GAWTC 3 cut(s) 442, 461, 496
Tru1I TTAA 1 cut(s) 543
Tru9I TTAA 1 cut(s) 543
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 2 cut(s) 135, 469
TseI GCWGC 2 cut(s) 118, 478
Tsp45I GTSAC 2 cut(s) 135, 469
TspDTI ATGAA 1 cut(s) 248
TspGWI ACGGA 1 cut(s) 39
TspRI CASTG 1 cut(s) 118
VneI GTGCAC 1 cut(s) 449
XceI RCATGY 1 cut(s) 245
XmiI GTMKAC 1 cut(s) 246
XspI CTAG 1 cut(s) 308
ZraI GACGTC 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.