pycom07g22010

Phosphodiesterase responsible for the U6 snRNA 3' end processing. Acts as an exoribonuclease (RNase) responsible for trimming the poly(U) tract of the last nucleotides in the pre-U6 snRNA molecule, leading to the formation of mature U6 snRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0006090
Physical Location & Seq
Forward (+)
23473487 .. 23474954
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
ATGAACAAGCTAGCTTCTCTGGTGCCTGGTCTCCATGCCGTTGACATTGACGTCCCGCTTGACATTCTGCGTAAGGACGAGCATAAACTTGAACAAGTTGCTTTAGGTAGAGAGTTCCATATAAGTCTCGGAAGAACTGTTCCGATTCGGGTGCACCAGATTGATTCCTTGGTGACAATGCTGCGGCAGAAGCTTCAGATTCAGAGGCGGTATTGGATTGATTTTAGCAAGTGGGAGGTTTTTGTTAACGATGATCAGATCCGGACCTTTGTGTCGATCGAAGTCATTGCTGCTGGGTCAGCTGAGATAACAAAGCAGATTCAAGCTGTGAATGAGGTGTATAAGCTTCACAATCTTCCTGAATTTTACAAGGATCCGCGCCCTCACATCTCGGTAGCTTGGGCATTGGGTGACATCAACAATTCCCTGAAGAAAGTGGTTGAAGAAGAAAGACGAATATCTACCATCGGAGGATCGTTACAGAAATGTATTTTTACCAGTAAATTCAATGGTATCGAATGTAGGATTGGTAATAAAACACAAAATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HVSL

Protein Analysis

184

Amino Acids

21.02

Weight (kDa)

8.89

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HVSL PF09749 34 - 182 3.4e-41 Uncharacterised conserved protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 50, 271
AatII GACGTC 1 cut(s) 54
AccB1I GGYRCC 1 cut(s) 22
AccII CGCG 1 cut(s) 379
AccIII TCCGGA 1 cut(s) 261
AciI CCGC 4 cut(s) 56, 184, 208, 377
AclWI GGATC 4 cut(s) 253, 368, 381, 481
AcsI RAATTY 2 cut(s) 362, 503
AcuI CTGAAG 2 cut(s) 179, 449
AcyI GRCGYC 1 cut(s) 51
AgsI TTSAA 4 cut(s) 92, 323, 443, 508
AjnI CCWGG 1 cut(s) 25
AjuI GAANNNNNNNTTGG 2 cut(s) 510, 542
AloI GAACNNNNNNTCC 2 cut(s) 123, 155
AluBI AGCT 7 cut(s) 10, 14, 193, 302, 326, 346, 398
AluI AGCT 7 cut(s) 10, 14, 193, 302, 326, 346, 398
Alw21I GWGCWC 1 cut(s) 156
Alw26I GTCTC 2 cut(s) 35, 131
Alw44I GTGCAC 1 cut(s) 152
AlwI GGATC 4 cut(s) 253, 368, 381, 481
Aor13HI TCCGGA 1 cut(s) 261
ApaLI GTGCAC 1 cut(s) 152
ApeKI GCWGC 2 cut(s) 181, 290
ApoI RAATTY 2 cut(s) 362, 503
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 1 cut(s) 264
AsuHPI GGTGA 2 cut(s) 184, 422
AsuNHI GCTAGC 1 cut(s) 10
AvaII GGWCC 1 cut(s) 264
BaeGI GKGCMC 1 cut(s) 156
BamHI GGATCC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 2 cut(s) 168, 277
BccI CCATC 1 cut(s) 473
BceAI ACGGC 1 cut(s) 23
BcgI CGANNNNNNTGC 4 cut(s) 133, 167, 269, 303
BciT130I CCWGG 1 cut(s) 27
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 2 cut(s) 35, 131
BfaI CTAG 1 cut(s) 11
BisI GCNGC 3 cut(s) 182, 185, 291
BlsI GCNGC 3 cut(s) 183, 186, 292
Bme1390I CCNGG 1 cut(s) 27
Bme18I GGWCC 1 cut(s) 264
BmgT120I GGNCC 1 cut(s) 264
BmiI GGNNCC 2 cut(s) 24, 375
BmrFI CCNGG 1 cut(s) 27
BmtI GCTAGC 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 168
BsaWI WCCGGW 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 498
Bse3DI GCAATG 1 cut(s) 285
BseAI TCCGGA 1 cut(s) 261
BseBI CCWGG 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 168
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 498
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 2 cut(s) 168, 277
BseYI CCCAGC 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 379
Bsh1285I CGRYCG 1 cut(s) 279
BshNI GGYRCC 1 cut(s) 22
BsiEI CGRYCG 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 156
BsiSI CCGG 1 cut(s) 262
BslFI GGGAC 1 cut(s) 38
BsmAI GTCTC 2 cut(s) 35, 131
BsmFI GGGAC 1 cut(s) 38
Bso31I GGTCTC 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 156
Bsp13I TCCGGA 1 cut(s) 261
Bsp143I GATC 5 cut(s) 253, 258, 276, 373, 473
BspACI CCGC 4 cut(s) 56, 184, 208, 377
BspCNI CTCAG 1 cut(s) 295
BspEI TCCGGA 1 cut(s) 261
BspFNI CGCG 1 cut(s) 379
BspLI GGNNCC 2 cut(s) 24, 375
BspOI GCTAGC 1 cut(s) 14
BspPI GGATC 4 cut(s) 253, 368, 381, 481
BspT107I GGYRCC 1 cut(s) 22
BspTNI GGTCTC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 1 cut(s) 498
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 5 cut(s) 253, 258, 276, 373, 473
BssNI GRCGYC 1 cut(s) 51
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 1 cut(s) 139
BstACI GRCGYC 1 cut(s) 51
BstC8I GCNNGC 1 cut(s) 12
BstDEI CTNAG 1 cut(s) 303
BstFNI CGCG 1 cut(s) 379
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 5 cut(s) 256, 261, 279, 376, 476
BstMAI GTCTC 2 cut(s) 35, 131
BstMBI GATC 5 cut(s) 253, 258, 276, 373, 473
BstMCI CGRYCG 1 cut(s) 279
BstMWI GCNNNNNNNGC 2 cut(s) 190, 299
BstNI CCWGG 1 cut(s) 27
BstSCI CCNGG 1 cut(s) 25
BstSLI GKGCMC 1 cut(s) 156
BstUI CGCG 1 cut(s) 379
BstV1I GCAGC 2 cut(s) 168, 277
BstX2I RGATCY 2 cut(s) 258, 373
BstYI RGATCY 2 cut(s) 258, 373
Cac8I GCNNGC 1 cut(s) 12
CfoI GCGC 1 cut(s) 381
Cfr13I GGNCC 1 cut(s) 264
CviAII CATG 1 cut(s) 35
CviJI RGCY 7 cut(s) 10, 14, 193, 302, 326, 346, 398
CviKI_1 RGCY 7 cut(s) 10, 14, 193, 302, 326, 346, 398
DdeI CTNAG 1 cut(s) 303
DpnI GATC 5 cut(s) 255, 260, 278, 375, 475
DpnII GATC 5 cut(s) 253, 258, 276, 373, 473
DrdI GACNNNNNNGTC 2 cut(s) 50, 271
DseDI GACNNNNNNGTC 2 cut(s) 50, 271
Eco130I CCWWGG 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 264
Eco57I CTGAAG 2 cut(s) 179, 449
EcoRII CCWGG 1 cut(s) 25
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 1 cut(s) 38
FaiI YATR 6 cut(s) 36, 84, 120, 122, 342, 548
FaqI GGGAC 1 cut(s) 38
FatI CATG 1 cut(s) 34
FauI CCCGC 1 cut(s) 63
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 3 cut(s) 182, 185, 291
Fsp4HI GCNGC 3 cut(s) 182, 185, 291
FspBI CTAG 1 cut(s) 11
GlaI GCGC 1 cut(s) 380
GluI GCNGC 3 cut(s) 182, 185, 291
GsaI CCCAGC 1 cut(s) 297
HapII CCGG 1 cut(s) 262
HhaI GCGC 1 cut(s) 381
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 1 cut(s) 38
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HincII GTYRAC 2 cut(s) 43, 247
HindII GTYRAC 2 cut(s) 43, 247
HindIII AAGCTT 2 cut(s) 191, 344
HinfI GANTC 4 cut(s) 145, 164, 199, 319
HpaI GTTAAC 1 cut(s) 247
HpaII CCGG 1 cut(s) 262
HphI GGTGA 2 cut(s) 184, 422
Hpy166II GTNNAC 3 cut(s) 43, 154, 247
Hpy188I TCNGA 6 cut(s) 131, 144, 198, 204, 258, 470
Hpy188III TCNNGA 2 cut(s) 262, 359
Hpy8I GTNNAC 3 cut(s) 43, 154, 247
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 1 cut(s) 154
HpyF10VI GCNNNNNNNGC 2 cut(s) 190, 299
HpyF3I CTNAG 1 cut(s) 303
HpySE526I ACGT 1 cut(s) 51
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 1 cut(s) 38
HspAI GCGC 1 cut(s) 379
Kpn2I TCCGGA 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 253
KspAI GTTAAC 1 cut(s) 247
Kzo9I GATC 5 cut(s) 253, 258, 276, 373, 473
LpnPI CCDG 9 cut(s) 5, 12, 39, 170, 275, 279, 372, 440, 511
Lsp1109I GCAGC 2 cut(s) 168, 277
MaeI CTAG 1 cut(s) 11
MaeII ACGT 1 cut(s) 51
MaeIII GTNAC 3 cut(s) 172, 410, 477
MalI GATC 5 cut(s) 255, 260, 278, 375, 475
MboI GATC 5 cut(s) 253, 258, 276, 373, 473
MboII GAAGA 5 cut(s) 144, 347, 442, 455, 458
MflI RGATCY 2 cut(s) 258, 373
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 3 cut(s) 362, 421, 503
MnlI CCTC 5 cut(s) 198, 229, 328, 393, 464
MroI TCCGGA 1 cut(s) 261
MseI TTAA 1 cut(s) 246
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 1 cut(s) 262
MspR9I CCNGG 1 cut(s) 27
MvaI CCWGG 1 cut(s) 27
MvnI CGCG 1 cut(s) 379
MwoI GCNNNNNNNGC 2 cut(s) 190, 299
NdeII GATC 5 cut(s) 253, 258, 276, 373, 473
NheI GCTAGC 1 cut(s) 10
NlaIII CATG 1 cut(s) 38
NlaIV GGNNCC 2 cut(s) 24, 375
NmuCI GTSAC 2 cut(s) 172, 410
PfeI GAWTC 4 cut(s) 145, 164, 199, 319
PkrI GCNGC 3 cut(s) 183, 186, 292
Ple19I CGATCG 1 cut(s) 279
Psp6I CCWGG 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 293
PspGI CCWGG 1 cut(s) 25
PspN4I GGNNCC 2 cut(s) 24, 375
PspPI GGNCC 1 cut(s) 264
PsuI RGATCY 2 cut(s) 258, 373
PvuI CGATCG 1 cut(s) 279
PvuII CAGCTG 1 cut(s) 302
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 3 cut(s) 182, 185, 291
Sau3AI GATC 5 cut(s) 253, 258, 276, 373, 473
Sau96I GGNCC 1 cut(s) 264
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 1 cut(s) 156
SinI GGWCC 1 cut(s) 264
Sse9I AATT 3 cut(s) 362, 421, 503
SsiI CCGC 4 cut(s) 56, 184, 208, 377
SspMI CTAG 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 1 cut(s) 139
TaiI ACGT 1 cut(s) 54
TaqI TCGA 3 cut(s) 275, 279, 516
TasI AATT 3 cut(s) 362, 421, 503
TauI GCSGC 1 cut(s) 187
TfiI GAWTC 4 cut(s) 145, 164, 199, 319
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TseFI GTSAC 2 cut(s) 172, 410
TseI GCWGC 2 cut(s) 181, 290
Tsp45I GTSAC 2 cut(s) 172, 410
TspDTI ATGAA 1 cut(s) 17
VneI GTGCAC 1 cut(s) 152
VpaK11BI GGWCC 1 cut(s) 264
XapI RAATTY 2 cut(s) 362, 503
XspI CTAG 1 cut(s) 11
ZraI GACGTC 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.