pycom07g22170

Zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0016042
Physical Location & Seq
Forward (+)
23570692 .. 23571594
903 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g22170.1

Sequence Viewer

Length: 903 bp
ATGATGCAAGGTGTGCATCCGGGTTACTACTACCAGCTGGTCGACCCGAGCGTGTACTATTCGGGTCTCCTCAGGAGTCCGGTCCAGTACCAGGGCCAGTACCACGACTCGATGATCCCCGTCCAGTACCAGTGCCAGTCCCACGACCCGATGATCGACAATGCCATCTTTGGGTCGGACGAATTCCGGATGTACGCTTACAAGGTGAAGCGTTGCCAACGCATGGGAGCCCACGACTGGACGAACTGCCCGTACGCTCACCGCGGCGAGAAGGCCCAGCGCCGTGACCCCCGCAAATTCGCCTACGCGGCGATTATTTGCCCGGCATTTCGCAGCACCGGTTACTGCCGCAAGGGAGACCGTTGCGAATGTGCCCACGGAGTGTTCGAGTATTGGCTCCATCCGGCCAAGTACCGCACGCGTGCATGCGCTAGTCTCGAGAATGGGTACTGCCCGCGCAAAGTGTGCTTCTTCGCTCATACGCCCGATGAGCTCCGGCCGCAACACACGTATTCCGGCCACAAGTACTATGTTGCTTATGATCAGCAAGCAGGTTTTTATCCTCCCTACCACTACTACAAGTACCAGAACAACAGCGACCTGTACCGTGCGGCCCCAATGACCCCGAAGGCCAACCGTGGAGGAGGAGGCGTGGCAACGGAAGCACCGTGGACGGCTGAGGAGTATCAGACAATGATGAACAACAAGAAGATCGTGGACGAGTCTTGCTTGAAGGTGGAGGAATTCCTGAACAGTCTGAGGGCGTTGAAGCTGAGTGACTATGAGATGGAGTATGACCAAGGTGAAATCGATGCTGCTTGTGGGATGAAGAGTTATGGTGGTGGGGAGGCGTCGGTGTCGGAGATGCCTCAGTTTGACTGGATTAACAAGCTGCTGCAGTAA

Protein Analysis

301

Amino Acids

34.73

Weight (kDa)

7.53

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_AtC3H23 PF25512 61 - 94 2.6e-17 AtC3H23-like, CCCH zinc finger
zf-CCCH PF00642 106 - 127 8.4e-06 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016042)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07500
fragaria_vesca FvH4_7g26870
malus_domestica MD07G1248000.v1.1
prunus_persica Prupe.2G272000_v2.0.a1
pyrus_communis pycom07g22170
rosa_chinensis RchiOBHm_Chr1g0373551
rosa_laevigata RLG00000026816
rosa_multiflora Rmu_sc0002860.1_g000004
rosa_roxburghii Rroxscaffold_4G00284150
rosa_rugosa Rorug01G0378100
rosa_samantha Rh1AG387600 Rh1BG351900 Rh1CG364600 Rh1DG382300
rosa_wichuraiana Rw1G034780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 542
AccB7I CCANNNNNTGG 1 cut(s) 223
AccI GTMKAC 1 cut(s) 42
AccII CGCG 4 cut(s) 264, 308, 421, 457
AccIII TCCGGA 1 cut(s) 186
AciI CCGC 9 cut(s) 262, 264, 292, 308, 349, 415, 455, 500, 611
AclWI GGATC 1 cut(s) 109
AcoI YGGCCR 3 cut(s) 405, 497, 517
AcsI RAATTY 3 cut(s) 182, 296, 743
AcyI GRCGYC 1 cut(s) 851
AdeI CACNNNGTG 1 cut(s) 382
AfiI CCNNNNNNNGG 4 cut(s) 91, 171, 223, 237
AflIII ACRYGT 2 cut(s) 419, 507
AgeI ACCGGT 1 cut(s) 338
AgsI TTSAA 2 cut(s) 733, 769
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 4 cut(s) 37, 493, 772, 892
AluI AGCT 4 cut(s) 37, 493, 772, 892
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 3 cut(s) 71, 351, 440
AlwI GGATC 1 cut(s) 109
Ama87I CYCGRG 2 cut(s) 46, 437
Aor13HI TCCGGA 1 cut(s) 186
AoxI GGCC 7 cut(s) 94, 273, 405, 497, 517, 612, 630
ApeKI GCWGC 4 cut(s) 333, 815, 892, 895
ApoI RAATTY 3 cut(s) 182, 296, 743
AsiGI ACCGGT 1 cut(s) 338
AspLEI GCGC 3 cut(s) 282, 431, 459
AspS9I GGNCC 4 cut(s) 82, 94, 274, 613
AsuC2I CCSGG 2 cut(s) 21, 323
AsuHPI GGTGA 3 cut(s) 217, 251, 815
AvaI CYCGRG 2 cut(s) 46, 437
AvaII GGWCC 1 cut(s) 82
AxyI CCTNAGG 1 cut(s) 71
BaeGI GKGCMC 1 cut(s) 376
BanII GRGCYC 2 cut(s) 232, 495
Bbv12I GWGCWC 1 cut(s) 495
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 4 cut(s) 345, 802, 879, 882
BccI CCATC 3 cut(s) 173, 408, 781
BceAI ACGGC 2 cut(s) 267, 690
BciT130I CCWGG 1 cut(s) 92
BclI TGATCA 1 cut(s) 541
BcnI CCSGG 2 cut(s) 21, 323
BcoDI GTCTC 3 cut(s) 71, 351, 440
BfaI CTAG 1 cut(s) 432
BfmI CTRYAG 1 cut(s) 896
BfoI RGCGCY 1 cut(s) 283
BfuAI ACCTGC 1 cut(s) 542
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 9 cut(s) 265, 309, 334, 349, 500, 612, 816, 893, 896
BlsI GCNGC 9 cut(s) 266, 310, 335, 350, 501, 613, 817, 894, 897
BmcAI AGTACT 1 cut(s) 527
Bme1390I CCNGG 3 cut(s) 21, 92, 323
Bme18I GGWCC 1 cut(s) 82
BmeT110I CYCGRG 2 cut(s) 46, 437
BmgT120I GGNCC 4 cut(s) 82, 94, 274, 613
BmiI GGNNCC 3 cut(s) 229, 398, 615
BmrFI CCNGG 3 cut(s) 21, 92, 323
BmsI GCATC 3 cut(s) 25, 802, 855
Bpu10I CCTNAGC 1 cut(s) 678
BpuMI CCSGG 2 cut(s) 21, 323
Bsa29I ATCGAT 1 cut(s) 810
BsaAI YACGTR 1 cut(s) 510
BsaHI GRCGYC 1 cut(s) 851
BsaI GGTCTC 2 cut(s) 71, 351
BsaJI CCNNGG 6 cut(s) 91, 262, 376, 637, 668, 799
BsaWI WCCGGW 3 cut(s) 79, 186, 338
BsaXI ACNNNNNCTCC 2 cut(s) 636, 666
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 171, 223, 237
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 7 cut(s) 85, 97, 124, 130, 136, 242, 884
Bse21I CCTNAGG 1 cut(s) 71
BseAI TCCGGA 1 cut(s) 186
BseBI CCWGG 1 cut(s) 92
BseCI ATCGAT 1 cut(s) 810
BseDI CCNNGG 6 cut(s) 91, 262, 376, 637, 668, 799
BseGI GGATG 4 cut(s) 16, 195, 400, 831
BseLI CCNNNNNNNGG 4 cut(s) 91, 171, 223, 237
BseMII CTCAG 5 cut(s) 85, 669, 749, 764, 884
BseNI ACTGG 7 cut(s) 85, 97, 124, 130, 136, 242, 884
BseRI GAGGAG 4 cut(s) 59, 657, 660, 695
BseSI GKGCMC 1 cut(s) 376
BseX3I CGGCCG 1 cut(s) 497
BseXI GCAGC 4 cut(s) 345, 802, 879, 882
BseYI CCCAGC 1 cut(s) 276
Bsh1236I CGCG 4 cut(s) 264, 308, 421, 457
Bsh1285I CGRYCG 1 cut(s) 500
BshFI GGCC 7 cut(s) 96, 275, 407, 499, 519, 614, 632
BshTI ACCGGT 1 cut(s) 338
BshVI ATCGAT 1 cut(s) 810
BsiEI CGRYCG 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 495
BsiHKCI CYCGRG 2 cut(s) 46, 437
BsiSI CCGG 8 cut(s) 20, 80, 187, 323, 339, 404, 496, 516
BsiWI CGTACG 1 cut(s) 252
BslFI GGGAC 1 cut(s) 124
BslI CCNNNNNNNGG 4 cut(s) 91, 171, 223, 237
BsmAI GTCTC 3 cut(s) 71, 351, 440
BsmFI GGGAC 1 cut(s) 124
BsnI GGCC 7 cut(s) 96, 275, 407, 499, 519, 614, 632
Bso31I GGTCTC 2 cut(s) 71, 351
BsoBI CYCGRG 2 cut(s) 46, 437
Bsp1286I GDGCHC 3 cut(s) 232, 376, 495
Bsp13I TCCGGA 1 cut(s) 186
Bsp143I GATC 4 cut(s) 114, 153, 541, 711
BspACI CCGC 9 cut(s) 262, 264, 292, 308, 349, 415, 455, 500, 611
BspANI GGCC 7 cut(s) 96, 275, 407, 499, 519, 614, 632
BspCNI CTCAG 5 cut(s) 84, 670, 750, 765, 883
BspDI ATCGAT 1 cut(s) 810
BspEI TCCGGA 1 cut(s) 186
BspFNI CGCG 4 cut(s) 264, 308, 421, 457
BspLI GGNNCC 3 cut(s) 229, 398, 615
BspMAI CTGCAG 1 cut(s) 900
BspMI ACCTGC 1 cut(s) 542
BspPI GGATC 1 cut(s) 109
BspTNI GGTCTC 2 cut(s) 71, 351
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 7 cut(s) 85, 97, 124, 130, 136, 242, 884
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 6 cut(s) 91, 262, 376, 637, 668, 799
BssMI GATC 4 cut(s) 114, 153, 541, 711
BssNI GRCGYC 1 cut(s) 851
BssT1I CCWWGG 1 cut(s) 799
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 5 cut(s) 362, 608, 638, 669, 755
Bst6I CTCTTC 1 cut(s) 824
BstACI GRCGYC 1 cut(s) 851
BstAPI GCANNNNNTGC 2 cut(s) 13, 465
BstBAI YACGTR 1 cut(s) 510
BstC8I GCNNGC 5 cut(s) 419, 423, 427, 455, 549
BstDEI CTNAG 5 cut(s) 71, 678, 758, 773, 870
BstDSI CCRYGG 4 cut(s) 262, 376, 637, 668
BstF5I GGATG 4 cut(s) 16, 195, 400, 831
BstFNI CGCG 4 cut(s) 264, 308, 421, 457
BstH2I RGCGCY 1 cut(s) 283
BstHHI GCGC 3 cut(s) 282, 431, 459
BstKTI GATC 4 cut(s) 117, 156, 544, 714
BstMAI GTCTC 3 cut(s) 71, 351, 440
BstMBI GATC 4 cut(s) 114, 153, 541, 711
BstMCI CGRYCG 1 cut(s) 500
BstMWI GCNNNNNNNGC 6 cut(s) 13, 308, 465, 490, 499, 662
BstNI CCWGG 1 cut(s) 92
BstNSI RCATGY 1 cut(s) 429
BstSCI CCNGG 3 cut(s) 19, 90, 321
BstSFI CTRYAG 1 cut(s) 896
BstSLI GKGCMC 1 cut(s) 376
BstUI CGCG 4 cut(s) 264, 308, 421, 457
BstV1I GCAGC 4 cut(s) 345, 802, 879, 882
BstZI CGGCCG 1 cut(s) 497
Bsu15I ATCGAT 1 cut(s) 810
Bsu36I CCTNAGG 1 cut(s) 71
BsuRI GGCC 7 cut(s) 96, 275, 407, 499, 519, 614, 632
BsuTUI ATCGAT 1 cut(s) 810
BtgI CCRYGG 4 cut(s) 262, 376, 637, 668
BtsCI GGATG 4 cut(s) 16, 195, 400, 831
BtsIMutI CAGTG 1 cut(s) 137
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 5 cut(s) 419, 423, 427, 455, 549
CfoI GCGC 3 cut(s) 282, 431, 459
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 4 cut(s) 82, 94, 274, 613
Cfr42I CCGCGG 1 cut(s) 265
ClaI ATCGAT 1 cut(s) 810
CseI GACGC 1 cut(s) 840
CspAI ACCGGT 1 cut(s) 338
CviAII CATG 2 cut(s) 223, 426
DdeI CTNAG 5 cut(s) 71, 678, 758, 773, 870
DpnI GATC 4 cut(s) 116, 155, 543, 713
DpnII GATC 4 cut(s) 114, 153, 541, 711
DraIII CACNNNGTG 1 cut(s) 382
EaeI YGGCCR 3 cut(s) 405, 497, 517
EagI CGGCCG 1 cut(s) 497
Eam1104I CTCTTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 824
Ecl136II GAGCTC 1 cut(s) 493
EclXI CGGCCG 1 cut(s) 497
Eco130I CCWWGG 1 cut(s) 799
Eco24I GRGCYC 2 cut(s) 232, 495
Eco31I GGTCTC 2 cut(s) 71, 351
Eco47I GGWCC 1 cut(s) 82
Eco52I CGGCCG 1 cut(s) 497
Eco53kI GAGCTC 1 cut(s) 493
Eco81I CCTNAGG 1 cut(s) 71
Eco88I CYCGRG 2 cut(s) 46, 437
EcoICRI GAGCTC 1 cut(s) 493
EcoRI GAATTC 2 cut(s) 182, 743
EcoRII CCWGG 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 799
EcoT38I GRGCYC 2 cut(s) 232, 495
ErhI CCWWGG 1 cut(s) 799
FaeI CATG 2 cut(s) 226, 429
FaiI YATR 8 cut(s) 224, 427, 480, 531, 540, 783, 795, 837
FaqI GGGAC 1 cut(s) 124
FatI CATG 2 cut(s) 222, 425
FauI CCCGC 2 cut(s) 299, 462
FbaI TGATCA 1 cut(s) 541
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 9 cut(s) 265, 309, 334, 349, 500, 612, 816, 893, 896
FokI GGATG 4 cut(s) 3, 202, 387, 838
FriOI GRGCYC 2 cut(s) 232, 495
Fsp4HI GCNGC 9 cut(s) 265, 309, 334, 349, 500, 612, 816, 893, 896
FspBI CTAG 1 cut(s) 432
GlaI GCGC 3 cut(s) 281, 430, 458
GluI GCNGC 9 cut(s) 265, 309, 334, 349, 500, 612, 816, 893, 896
GsaI CCCAGC 1 cut(s) 280
HaeII RGCGCY 1 cut(s) 283
HaeIII GGCC 7 cut(s) 96, 275, 407, 499, 519, 614, 632
HapII CCGG 8 cut(s) 20, 80, 187, 323, 339, 404, 496, 516
HgaI GACGC 1 cut(s) 840
HhaI GCGC 3 cut(s) 282, 431, 459
Hin1I GRCGYC 1 cut(s) 851
Hin1II CATG 2 cut(s) 226, 429
Hin6I GCGC 3 cut(s) 280, 429, 457
HinP1I GCGC 3 cut(s) 280, 429, 457
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 3 cut(s) 76, 107, 722
HpaII CCGG 8 cut(s) 20, 80, 187, 323, 339, 404, 496, 516
HphI GGTGA 3 cut(s) 217, 251, 815
Hpy166II GTNNAC 4 cut(s) 43, 55, 672, 718
Hpy188I TCNGA 4 cut(s) 178, 690, 759, 862
Hpy188III TCNNGA 5 cut(s) 73, 187, 437, 439, 748
Hpy8I GTNNAC 4 cut(s) 43, 55, 672, 718
Hpy99I CGWCG 1 cut(s) 856
HpyAV CCTTC 3 cut(s) 265, 622, 727
HpyCH4III ACNGT 5 cut(s) 362, 608, 638, 669, 755
HpyCH4IV ACGT 1 cut(s) 509
HpyCH4V TGCA 4 cut(s) 7, 16, 425, 898
HpyF10VI GCNNNNNNNGC 6 cut(s) 13, 308, 465, 490, 499, 662
HpyF3I CTNAG 5 cut(s) 71, 678, 758, 773, 870
HpySE526I ACGT 1 cut(s) 509
Hsp92I GRCGYC 1 cut(s) 851
Hsp92II CATG 2 cut(s) 226, 429
HspAI GCGC 3 cut(s) 280, 429, 457
Kpn2I TCCGGA 1 cut(s) 186
Ksp22I TGATCA 1 cut(s) 541
KspI CCGCGG 1 cut(s) 265
Kzo9I GATC 4 cut(s) 114, 153, 541, 711
LmnI GCTCC 3 cut(s) 227, 402, 498
Lsp1109I GCAGC 4 cut(s) 345, 802, 879, 882
LweI GCATC 3 cut(s) 25, 802, 855
MaeI CTAG 1 cut(s) 432
MaeII ACGT 1 cut(s) 509
MaeIII GTNAC 4 cut(s) 23, 284, 341, 776
MalI GATC 4 cut(s) 116, 155, 543, 713
MboI GATC 4 cut(s) 114, 153, 541, 711
MboII GAAGA 3 cut(s) 463, 721, 841
MhlI GDGCHC 3 cut(s) 232, 376, 495
MluCI AATT 3 cut(s) 182, 296, 743
MluI ACGCGT 1 cut(s) 419
MlyI GAGTC 3 cut(s) 85, 101, 731
MmeI TCCRAC 2 cut(s) 156, 840
MroI TCCGGA 1 cut(s) 186
MseI TTAA 1 cut(s) 885
MspA1I CMGCKG 2 cut(s) 37, 264
MspI CCGG 8 cut(s) 20, 80, 187, 323, 339, 404, 496, 516
MspR9I CCNGG 3 cut(s) 21, 92, 323
MvaI CCWGG 1 cut(s) 92
MvnI CGCG 4 cut(s) 264, 308, 421, 457
MwoI GCNNNNNNNGC 6 cut(s) 13, 308, 465, 490, 499, 662
NciI CCSGG 2 cut(s) 21, 323
NdeII GATC 4 cut(s) 114, 153, 541, 711
NlaIII CATG 2 cut(s) 226, 429
NlaIV GGNNCC 3 cut(s) 229, 398, 615
NmuCI GTSAC 2 cut(s) 284, 776
NspI RCATGY 1 cut(s) 429
PaeI GCATGC 1 cut(s) 429
PaeR7I CTCGAG 1 cut(s) 437
PcsI WCGNNNNNNNCGW 4 cut(s) 48, 248, 384, 665
Pfl23II CGTACG 1 cut(s) 252
PflMI CCANNNNNTGG 1 cut(s) 223
PinAI ACCGGT 1 cut(s) 338
PkrI GCNGC 9 cut(s) 266, 310, 335, 350, 501, 613, 817, 894, 897
PleI GAGTC 3 cut(s) 84, 101, 730
PpsI GAGTC 3 cut(s) 84, 101, 730
Ppu21I YACGTR 1 cut(s) 510
Psp124BI GAGCTC 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 90
PspFI CCCAGC 1 cut(s) 276
PspGI CCWGG 1 cut(s) 90
PspLI CGTACG 1 cut(s) 252
PspN4I GGNNCC 3 cut(s) 229, 398, 615
PspPI GGNCC 4 cut(s) 82, 94, 274, 613
PsrI GAACNNNNNNTAC 2 cut(s) 236, 268
PstI CTGCAG 1 cut(s) 900
PvuII CAGCTG 1 cut(s) 37
SacI GAGCTC 1 cut(s) 495
SacII CCGCGG 1 cut(s) 265
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 885
SatI GCNGC 9 cut(s) 265, 309, 334, 349, 500, 612, 816, 893, 896
Sau3AI GATC 4 cut(s) 114, 153, 541, 711
Sau96I GGNCC 4 cut(s) 82, 94, 274, 613
ScaI AGTACT 1 cut(s) 527
SchI GAGTC 3 cut(s) 85, 101, 731
ScrFI CCNGG 3 cut(s) 21, 92, 323
SduI GDGCHC 3 cut(s) 232, 376, 495
SfaNI GCATC 3 cut(s) 25, 802, 855
SfcI CTRYAG 1 cut(s) 896
Sfr274I CTCGAG 1 cut(s) 437
Sfr303I CCGCGG 1 cut(s) 265
SgrBI CCGCGG 1 cut(s) 265
SinI GGWCC 1 cut(s) 82
SlaI CTCGAG 1 cut(s) 437
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SphI GCATGC 1 cut(s) 429
Sse9I AATT 3 cut(s) 182, 296, 743
SsiI CCGC 9 cut(s) 262, 264, 292, 308, 349, 415, 455, 500, 611
SspMI CTAG 1 cut(s) 432
SstI GAGCTC 1 cut(s) 495
StyD4I CCNGG 3 cut(s) 19, 90, 321
StyI CCWWGG 1 cut(s) 799
TaaI ACNGT 5 cut(s) 362, 608, 638, 669, 755
TaiI ACGT 1 cut(s) 512
TaqI TCGA 6 cut(s) 42, 110, 156, 387, 438, 810
TasI AATT 3 cut(s) 182, 296, 743
TatI WGTACW 2 cut(s) 54, 525
TauI GCSGC 5 cut(s) 267, 311, 351, 502, 614
Tru1I TTAA 1 cut(s) 885
Tru9I TTAA 1 cut(s) 885
TscAI CASTG 1 cut(s) 137
TseFI GTSAC 2 cut(s) 284, 776
TseI GCWGC 4 cut(s) 333, 815, 892, 895
Tsp45I GTSAC 2 cut(s) 284, 776
TspDTI ATGAA 2 cut(s) 713, 842
TspGWI ACGGA 2 cut(s) 393, 674
TspRI CASTG 1 cut(s) 137
Van91I CCANNNNNTGG 1 cut(s) 223
VpaK11BI GGWCC 1 cut(s) 82
XapI RAATTY 3 cut(s) 182, 296, 743
XceI RCATGY 1 cut(s) 429
XhoI CTCGAG 1 cut(s) 437
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 1 cut(s) 432
ZrmI AGTACT 1 cut(s) 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.