pycom07g25330

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0007188
Physical Location & Seq
Forward (+)
25680187 .. 25682652
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g25330.1

Sequence Viewer

Length: 2466 bp
ATGGACGCCATGAACCTCCACTTTCTCTCTCTAACCCTGCTCTTTCTCCTCCACTTCGCAGCCTCCTTCACTCCCACCGATAACTACCTCATCAACTGCGGCTCACCCTCCAACGCCTCCGTCTTCAACCGGGTCTTCTCGGCGGACCCTTACAAACCCGGAGCGGCGGGATCCATTACGCTTGCCGACCAGAACCCACCTCCCAGTTCGCCTGCCCTCTACCGCACGGCCAGAGTTTTCACCAGGGCTTCGAGCTACACCTTCAGCATCAAGAAATCCGGGACCCACTTGGTACGTTTTCACTTCTCGCCGTTTGTAGCTCAGGGTTTTGACTTGAAAGCTGCAAACTTTGGCGTTTCCGTTGATGGGTTTGTTCTGTTGAGAGAATTACATGTTAGGGACTCTGTGATTAGAGAGTTTGTGATGAGAATTGATGCAAATGTGGTTGAAATTGTGTTTGCGCCTGTGGGCAATTCTGGGTTTTGCTATGTAAATGCAATTGAAGTTTTTACAGCTCCTGAGGATCTTGTTCTTGATTACGGTGCTAAATTGGTGGGTGCGAATGTTGTCGAATACAAGAATCTTTCTTCACAAGTTTTAGAGACCCTTTATAGGATTAATGTTGGAGGTTCGAAATTGACACCTTTTAATGATACTTTGTGGAGGGATTGGGTCCCTGATGTCGATTATCTTGTTCTGAAATCGGCTGCGAAGCGCGCTTCCACCACGCATACTCCGAATTATCAGAGGGGCGGTGCAACTCGAGAGATTGCCCCGGATAATGTATATATGACTGCCCAGGAGATGAATACGGATAAGGCAATTACAGGTGCAAGGTTTAATCTCACATGGAAATTTCCCGTGAATACGAATTCCGGGCATTTGGTTAGGTTGCACTTCTGTGACATTGTTAGTCCTGCACTTAATTTGTTGTACTTTAATGTGTATATCAACGGGTATGCTGCGTATAGAGATGTTGATCTATCCGTGCTGGCAACCAACGTGCTTGCATCTCCTCTCTATATTGATTTTGTTGTGGATTCAGATAGTTCGGGGATGATAGAAATCAGTGTTGGTCCTTCTGATCTGAGTGGTTCCATGAGGATTAATGCTATATTGAATGGGGCAGAGATCATGAGAATGGTGAATGCTTCCAATTTACGGGCTGGAACTGGGCGTAAGAAGAGAAGTATATGGATTTTGGTGGCCACAGTTGTTGGAGGCTTTGTTGTTCTCTGTTTTGCAATTGGTGTATTTCTGCTTGCTTTGAAACGCAGGAAGAAGAGACTGAAACCCCAACCTGCAGAAAGTGCGATTTGGACACCTTTACGCGTATATGGAGGTAGTTCAAACAGTAGAACGTCTGAAAGGACTACACTTGTGTCTCCCGGCACAAATGGATATCATTTCCTGAAAATATCTTTTGCTGAATTACAGTTGGCGACAAACAATTTCGATAAAAGTCTAATTGTAGGCTCTGGTGGTTTTGGCATGGTTTACAAAGGGGTCCTGAAGGACAACACAAAGGTTGCCGTGAAGAGAGGTGTGCCTGGATCTCGGCAGGGCCTTCCAGAATTCCAGACTGAAATAACGGTTTTGTCCCAAATTCGCCACCACCACCTTGTATCACTTGTTGGGTATTGTGAAGAACAGTCTGAAATGATCCTAGTTTATGAATATGTGGAAAAGGGGCCGTTGAAGAAACATTTGTATGGTTCTGGGTTTCCACCTTTATCCTGGAAACAACGGCTTGAAATATGCATTGGATCAGCGAGAGGTCTTCACTACCTTCATACAGGTTTTGCTCAAGGAATCATCCACCGTGACATTAAATCAACTAACATTTTGCTTGACGAGAATTATGTACCTAAGGTGGCTGATTTTGGTCTTTCAAGATCAGGTCCGTGTCTCAGTGAAACCCATGTAAGTACCGCTGTTAAAGGTAGTTTCGGGTATCTTGATCCTGAGTATTTCCGGAGGCAGCAGCTTACTGATAAGTCAGATGTGTATTCATTTGGGGTTGTGCTCTTTGAGGTTCTTTGCGCTAGACCTGCTGTCGATCCCTCAGTTGATAGGGAGCAAGTGAATCTAGGTGAATGGGCATTGGAATGGCAGAAGAAGGGCATGCTCGAGAAAATTATAGATCGCCATCTTGTTGGGAAGATCAAACCAAGCTCTTTGAAAAAGTTTGGAGAAACGGCAGAGAAATGTTTGGCTGAATATGGTGCGGATAGGCCAACCATGGGTGATGTATTATGGAATTTAGAATACGCGCTTCAGCTTCAGGAATCTGGACCGCGTGGAGGACAGCCTCAAGACTGCGATATCAATGTGCCTCCAACGAATAGCATCGTTCCTGGAGATCCTTCTGCCAATGTAAGAACAGAGGGAACTGATGGTAATGGTAGTTCGGAGATTAACACAAGCCTAGTTTTTTCTCAATTGATGACCAACGATGGCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0044464 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase_Tyr

Protein Analysis

822

Amino Acids

90.44

Weight (kDa)

8.45

Isoelectric Point (pI)

30.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 29 - 138 5.1e-07 Malectin domain
Malectin_like PF12819 31 - 380 2.6e-25 Malectin-like domain
Malectin PF11721 204 - 349 4.4e-06 Malectin domain
Pkinase PF00069 489 - 751 7.6e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 491 - 752 4.4e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1309, 2059
AccBSI CCGCTC 1 cut(s) 164
AccII CGCG 4 cut(s) 717, 1332, 2273, 2299
AccIII TCCGGA 1 cut(s) 1974
AciI CCGC 9 cut(s) 99, 143, 164, 167, 223, 753, 1932, 2228, 2297
AclWI GGATC 9 cut(s) 165, 178, 531, 1561, 1657, 1774, 1955, 2054, 2357
AcoI YGGCCR 2 cut(s) 228, 1206
AcsI RAATTY 5 cut(s) 854, 871, 1574, 1605, 2260
AcuI CTGAAG 4 cut(s) 247, 1532, 2261, 2267
AcyI GRCGYC 1 cut(s) 6
AfaI GTAC 4 cut(s) 294, 935, 1866, 1930
AfiI CCNNNNNNNGG 5 cut(s) 366, 612, 1161, 2243, 2303
AflIII ACRYGT 2 cut(s) 391, 1330
AhdI GACNNNNNGTC 1 cut(s) 2054
AjnI CCWGG 5 cut(s) 242, 798, 1549, 1736, 2356
AjuI GAANNNNNNNTTGG 4 cut(s) 1056, 1088, 1746, 1778
AleI CACNNNNGTG 1 cut(s) 900
AluBI AGCT 7 cut(s) 255, 320, 341, 515, 1987, 2175, 2281
AluI AGCT 7 cut(s) 255, 320, 341, 515, 1987, 2175, 2281
Alw21I GWGCWC 1 cut(s) 2028
Alw26I GTCTC 4 cut(s) 596, 1279, 1389, 1913
AlwI GGATC 9 cut(s) 165, 178, 531, 1561, 1657, 1774, 1955, 2054, 2357
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 2 cut(s) 762, 2129
Aor13HI TCCGGA 1 cut(s) 1974
AoxI GGCC 5 cut(s) 228, 1206, 1564, 1691, 2234
ApeKI GCWGC 6 cut(s) 59, 341, 707, 962, 1981, 1984
ApoI RAATTY 5 cut(s) 854, 871, 1574, 1605, 2260
AseI ATTAAT 2 cut(s) 618, 1107
AspLEI GCGC 5 cut(s) 463, 717, 719, 2045, 2275
AspS9I GGNCC 9 cut(s) 145, 282, 673, 1076, 1507, 1564, 1691, 1901, 2294
AsuC2I CCSGG 6 cut(s) 131, 159, 280, 776, 877, 1389
AsuHPI GGTGA 5 cut(s) 96, 232, 1156, 2105, 2258
AsuII TTCGAA 1 cut(s) 632
AvaI CYCGRG 2 cut(s) 762, 2129
AvaII GGWCC 7 cut(s) 145, 282, 673, 1076, 1507, 1901, 2294
AxyI CCTNAGG 2 cut(s) 519, 1869
BalI TGGCCA 1 cut(s) 1208
BamHI GGATCC 1 cut(s) 170
BbsI GAAGAC 3 cut(s) 115, 127, 1772
Bbv12I GWGCWC 1 cut(s) 2028
BbvI GCAGC 6 cut(s) 71, 328, 694, 949, 1993, 1996
BccI CCATC 4 cut(s) 359, 2157, 2390, 2450
BceAI ACGGC 6 cut(s) 243, 295, 1517, 1678, 1763, 2214
BciT130I CCWGG 5 cut(s) 244, 800, 1551, 1738, 2358
BcnI CCSGG 6 cut(s) 131, 159, 280, 776, 877, 1389
BcoDI GTCTC 4 cut(s) 596, 1279, 1389, 1913
BfaI CTAG 4 cut(s) 1667, 2046, 2090, 2429
BfmI CTRYAG 1 cut(s) 1302
BfuAI ACCTGC 2 cut(s) 1309, 2059
BisI GCNGC 8 cut(s) 60, 100, 165, 342, 708, 963, 1982, 1985
BlsI GCNGC 8 cut(s) 61, 101, 166, 343, 709, 964, 1983, 1986
Bme18I GGWCC 7 cut(s) 145, 282, 673, 1076, 1507, 1901, 2294
BmeRI GACNNNNNGTC 1 cut(s) 2054
BmeT110I CYCGRG 2 cut(s) 762, 2129
BmgT120I GGNCC 9 cut(s) 145, 282, 673, 1076, 1507, 1564, 1691, 1901, 2294
BmiI GGNNCC 9 cut(s) 147, 172, 283, 284, 674, 675, 1096, 1508, 1692
BmrI ACTGGG 2 cut(s) 198, 1182
BmsI GCATC 4 cut(s) 276, 424, 1019, 2358
BmuI ACTGGG 2 cut(s) 198, 1182
BpiI GAAGAC 3 cut(s) 115, 127, 1772
BpmI CTGGAG 1 cut(s) 2379
Bpu10I CCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 632
BpuEI CTTGAG 2 cut(s) 1791, 2298
BpuMI CCSGG 6 cut(s) 131, 159, 280, 776, 877, 1389
BsaBI GATNNNNATC 3 cut(s) 978, 1064, 2148
BsaHI GRCGYC 1 cut(s) 6
BsaI GGTCTC 1 cut(s) 596
BsaJI CCNNGG 4 cut(s) 243, 774, 798, 2241
BsaWI WCCGGW 1 cut(s) 1974
BsaXI ACNNNNNCTCC 4 cut(s) 718, 748, 1212, 1242
Bsc4I CCNNNNNNNGG 5 cut(s) 366, 612, 1161, 2243, 2303
Bse1I ACTGG 2 cut(s) 204, 1177
Bse21I CCTNAGG 2 cut(s) 519, 1869
Bse8I GATNNNNATC 3 cut(s) 978, 1064, 2148
BseAI TCCGGA 1 cut(s) 1974
BseBI CCWGG 5 cut(s) 244, 800, 1551, 1738, 2358
BseDI CCNNGG 4 cut(s) 243, 774, 798, 2241
BseGI GGATG 2 cut(s) 1062, 1815
BseJI GATNNNNATC 3 cut(s) 978, 1064, 2148
BseLI CCNNNNNNNGG 5 cut(s) 366, 612, 1161, 2243, 2303
BseMII CTCAG 6 cut(s) 335, 510, 1079, 1924, 1956, 2079
BseNI ACTGG 2 cut(s) 204, 1177
BsePI GCGCGC 1 cut(s) 715
BseRI GAGGAG 2 cut(s) 38, 1005
BseXI GCAGC 6 cut(s) 71, 328, 694, 949, 1993, 1996
BsgI GTGCAG 1 cut(s) 903
Bsh1236I CGCG 4 cut(s) 717, 1332, 2273, 2299
BshFI GGCC 5 cut(s) 230, 1208, 1566, 1693, 2236
BsiHKAI GWGCWC 1 cut(s) 2028
BsiHKCI CYCGRG 2 cut(s) 762, 2129
BsiSI CCGG 7 cut(s) 130, 159, 279, 776, 876, 1389, 1975
BslFI GGGAC 4 cut(s) 295, 413, 659, 1585
BslI CCNNNNNNNGG 5 cut(s) 366, 612, 1161, 2243, 2303
BsmAI GTCTC 4 cut(s) 596, 1279, 1389, 1913
BsmFI GGGAC 4 cut(s) 295, 413, 659, 1585
BsmI GAATGC 1 cut(s) 1153
BsnI GGCC 5 cut(s) 230, 1208, 1566, 1693, 2236
Bso31I GGTCTC 1 cut(s) 596
BsoBI CYCGRG 2 cut(s) 762, 2129
Bsp119I TTCGAA 1 cut(s) 632
Bsp1286I GDGCHC 1 cut(s) 2028
Bsp13I TCCGGA 1 cut(s) 1974
Bsp19I CCATGG 1 cut(s) 2241
BspACI CCGC 9 cut(s) 99, 143, 164, 167, 223, 753, 1932, 2228, 2297
BspANI GGCC 5 cut(s) 230, 1208, 1566, 1693, 2236
BspCNI CTCAG 6 cut(s) 334, 511, 1080, 1923, 1957, 2078
BspEI TCCGGA 1 cut(s) 1974
BspFNI CGCG 4 cut(s) 717, 1332, 2273, 2299
BspHI TCATGA 1 cut(s) 1134
BspLI GGNNCC 9 cut(s) 147, 172, 283, 284, 674, 675, 1096, 1508, 1692
BspMAI CTGCAG 1 cut(s) 1306
BspMI ACCTGC 2 cut(s) 1309, 2059
BspPI GGATC 9 cut(s) 165, 178, 531, 1561, 1657, 1774, 1955, 2054, 2357
BspT104I TTCGAA 1 cut(s) 632
BspTNI GGTCTC 1 cut(s) 596
BsrBI CCGCTC 1 cut(s) 164
BsrI ACTGG 2 cut(s) 204, 1177
BssECI CCNNGG 4 cut(s) 243, 774, 798, 2241
BssHII GCGCGC 1 cut(s) 715
BssNI GRCGYC 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 2241
Bst2UI CCWGG 5 cut(s) 244, 800, 1551, 1738, 2358
Bst4CI ACNGT 7 cut(s) 542, 1213, 1355, 1437, 1594, 1653, 1823
Bst6I CTCTTC 3 cut(s) 1178, 1277, 1532
BstACI GRCGYC 1 cut(s) 6
BstAPI GCANNNNNTGC 1 cut(s) 1310
BstBI TTCGAA 1 cut(s) 632
BstC8I GCNNGC 7 cut(s) 183, 213, 717, 993, 1008, 1263, 2126
BstDEI CTNAG 7 cut(s) 321, 519, 1088, 1869, 1910, 1965, 2065
BstDSI CCRYGG 1 cut(s) 2241
BstF5I GGATG 2 cut(s) 1062, 1815
BstFNI CGCG 4 cut(s) 717, 1332, 2273, 2299
BstHHI GCGC 5 cut(s) 463, 717, 719, 2045, 2275
BstMAI GTCTC 4 cut(s) 596, 1279, 1389, 1913
BstMWI GCNNNNNNNGC 3 cut(s) 716, 1310, 2051
BstNI CCWGG 5 cut(s) 244, 800, 1551, 1738, 2358
BstNSI RCATGY 2 cut(s) 395, 2128
BstSFI CTRYAG 1 cut(s) 1302
BstUI CGCG 4 cut(s) 717, 1332, 2273, 2299
BstV1I GCAGC 6 cut(s) 71, 328, 694, 949, 1993, 1996
BstV2I GAAGAC 3 cut(s) 115, 127, 1772
BstX2I RGATCY 4 cut(s) 170, 523, 1553, 2362
BstXI CCANNNNNNTGG 1 cut(s) 2156
BstYI RGATCY 4 cut(s) 170, 523, 1553, 2362
Bsu36I CCTNAGG 2 cut(s) 519, 1869
BsuRI GGCC 5 cut(s) 230, 1208, 1566, 1693, 2236
BtgI CCRYGG 1 cut(s) 2241
BtsCI GGATG 2 cut(s) 1062, 1815
BtsIMutI CAGTG 2 cut(s) 1075, 1918
BveI ACCTGC 2 cut(s) 1309, 2059
Cac8I GCNNGC 7 cut(s) 183, 213, 717, 993, 1008, 1263, 2126
CaiI CAGNNNCTG 1 cut(s) 518
CciI TCATGA 1 cut(s) 1134
CfoI GCGC 5 cut(s) 463, 717, 719, 2045, 2275
Cfr13I GGNCC 9 cut(s) 145, 282, 673, 1076, 1507, 1564, 1691, 1901, 2294
CseI GACGC 1 cut(s) 14
Csp6I GTAC 4 cut(s) 293, 934, 1865, 1929
CspCI CAANNNNNGTGG 2 cut(s) 1198, 1233
CviAII CATG 9 cut(s) 10, 392, 849, 1099, 1135, 1492, 1922, 2125, 2242
CviQI GTAC 4 cut(s) 293, 934, 1865, 1929
DdeI CTNAG 7 cut(s) 321, 519, 1088, 1869, 1910, 1965, 2065
DriI GACNNNNNGTC 1 cut(s) 2054
EaeI YGGCCR 2 cut(s) 228, 1206
Eam1104I CTCTTC 3 cut(s) 1178, 1277, 1532
Eam1105I GACNNNNNGTC 1 cut(s) 2054
EarI CTCTTC 3 cut(s) 1178, 1277, 1532
EciI GGCGGA 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 2241
Eco31I GGTCTC 1 cut(s) 596
Eco32I GATATC 2 cut(s) 1403, 2326
Eco47I GGWCC 7 cut(s) 145, 282, 673, 1076, 1507, 1901, 2294
Eco57I CTGAAG 4 cut(s) 247, 1532, 2261, 2267
Eco81I CCTNAGG 2 cut(s) 519, 1869
Eco88I CYCGRG 2 cut(s) 762, 2129
EcoO109I RGGNCCY 4 cut(s) 282, 673, 1507, 1564
EcoRI GAATTC 2 cut(s) 871, 1574
EcoRII CCWGG 5 cut(s) 242, 798, 1549, 1736, 2356
EcoRV GATATC 2 cut(s) 1403, 2326
EcoT14I CCWWGG 1 cut(s) 2241
EcoT22I ATGCAT 1 cut(s) 1763
ErhI CCWWGG 1 cut(s) 2241
FaeI CATG 9 cut(s) 13, 395, 852, 1102, 1138, 1495, 1925, 2128, 2245
FaqI GGGAC 4 cut(s) 295, 413, 659, 1585
FatI CATG 9 cut(s) 9, 391, 848, 1098, 1134, 1491, 1921, 2124, 2241
FauI CCCGC 1 cut(s) 160
Fnu4HI GCNGC 8 cut(s) 60, 100, 165, 342, 708, 963, 1982, 1985
FokI GGATG 2 cut(s) 1069, 1802
Fsp4HI GCNGC 8 cut(s) 60, 100, 165, 342, 708, 963, 1982, 1985
FspBI CTAG 4 cut(s) 1667, 2046, 2090, 2429
GlaI GCGC 5 cut(s) 462, 716, 718, 2044, 2274
GluI GCNGC 8 cut(s) 60, 100, 165, 342, 708, 963, 1982, 1985
GsuI CTGGAG 1 cut(s) 2379
HaeIII GGCC 5 cut(s) 230, 1208, 1566, 1693, 2236
HapII CCGG 7 cut(s) 130, 159, 279, 776, 876, 1389, 1975
HgaI GACGC 1 cut(s) 14
HhaI GCGC 5 cut(s) 463, 717, 719, 2045, 2275
Hin1I GRCGYC 1 cut(s) 6
Hin1II CATG 9 cut(s) 13, 395, 852, 1102, 1138, 1495, 1925, 2128, 2245
Hin6I GCGC 5 cut(s) 461, 715, 717, 2043, 2273
HinP1I GCGC 5 cut(s) 461, 715, 717, 2043, 2273
HinfI GANTC 6 cut(s) 401, 580, 1040, 1812, 2086, 2288
HpaII CCGG 7 cut(s) 130, 159, 279, 776, 876, 1389, 1975
HphI GGTGA 5 cut(s) 96, 232, 1156, 2105, 2258
Hpy166II GTNNAC 1 cut(s) 1498
Hpy8I GTNNAC 1 cut(s) 1498
HpyAV CCTTC 8 cut(s) 76, 271, 1089, 1507, 1577, 1799, 2113, 2376
HpyCH4III ACNGT 7 cut(s) 542, 1213, 1355, 1437, 1594, 1653, 1823
HpyCH4IV ACGT 3 cut(s) 295, 1002, 1361
HpyF10VI GCNNNNNNNGC 3 cut(s) 716, 1310, 2051
HpyF3I CTNAG 7 cut(s) 321, 519, 1088, 1869, 1910, 1965, 2065
HpySE526I ACGT 3 cut(s) 295, 1002, 1361
Hsp92I GRCGYC 1 cut(s) 6
Hsp92II CATG 9 cut(s) 13, 395, 852, 1102, 1138, 1495, 1925, 2128, 2245
HspAI GCGC 5 cut(s) 461, 715, 717, 2043, 2273
KflI GGGWCCC 2 cut(s) 282, 673
Kpn2I TCCGGA 1 cut(s) 1974
LmnI GCTCC 3 cut(s) 161, 520, 2077
Lsp1109I GCAGC 6 cut(s) 71, 328, 694, 949, 1993, 1996
LweI GCATC 4 cut(s) 276, 424, 1019, 2358
MaeI CTAG 4 cut(s) 1667, 2046, 2090, 2429
MaeII ACGT 3 cut(s) 295, 1002, 1361
MaeIII GTNAC 2 cut(s) 902, 1823
MbiI CCGCTC 1 cut(s) 164
MfeI CAATTG 3 cut(s) 498, 1245, 2441
MflI RGATCY 4 cut(s) 170, 523, 1553, 2362
MhlI GDGCHC 1 cut(s) 2028
MlsI TGGCCA 1 cut(s) 1208
MluI ACGCGT 1 cut(s) 1330
MluNI TGGCCA 1 cut(s) 1208
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 4 cut(s) 135, 604, 1198, 2363
Mox20I TGGCCA 1 cut(s) 1208
Mph1103I ATGCAT 1 cut(s) 1763
MroI TCCGGA 1 cut(s) 1974
MscI TGGCCA 1 cut(s) 1208
MseI TTAA 9 cut(s) 618, 648, 840, 924, 939, 1107, 1830, 1938, 2418
MslI CAYNNNNRTG 4 cut(s) 900, 1139, 1710, 2107
Msp20I TGGCCA 1 cut(s) 1208
MspA1I CMGCKG 1 cut(s) 1934
MspI CCGG 7 cut(s) 130, 159, 279, 776, 876, 1389, 1975
MunI CAATTG 3 cut(s) 498, 1245, 2441
Mva1269I GAATGC 1 cut(s) 1153
MvaI CCWGG 5 cut(s) 244, 800, 1551, 1738, 2358
MvnI CGCG 4 cut(s) 717, 1332, 2273, 2299
MwoI GCNNNNNNNGC 3 cut(s) 716, 1310, 2051
NciI CCSGG 6 cut(s) 131, 159, 280, 776, 877, 1389
NcoI CCATGG 1 cut(s) 2241
NlaIII CATG 9 cut(s) 13, 395, 852, 1102, 1138, 1495, 1925, 2128, 2245
NlaIV GGNNCC 9 cut(s) 147, 172, 283, 284, 674, 675, 1096, 1508, 1692
NmeAIII GCCGAG 2 cut(s) 119, 1537
NmuCI GTSAC 2 cut(s) 902, 1823
NsiI ATGCAT 1 cut(s) 1763
NspI RCATGY 2 cut(s) 395, 2128
NspV TTCGAA 1 cut(s) 632
OliI CACNNNNGTG 1 cut(s) 900
PaeI GCATGC 1 cut(s) 2128
PaeR7I CTCGAG 2 cut(s) 762, 2129
PagI TCATGA 1 cut(s) 1134
PauI GCGCGC 1 cut(s) 715
PciI ACATGT 1 cut(s) 391
PcsI WCGNNNNNNNCGW 1 cut(s) 734
PctI GAATGC 1 cut(s) 1153
PfeI GAWTC 5 cut(s) 580, 1040, 1812, 2086, 2288
PfoI TCCNGGA 3 cut(s) 278, 1736, 2356
PkrI GCNGC 8 cut(s) 61, 101, 166, 343, 709, 964, 1983, 1986
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
PpuMI RGGWCCY 3 cut(s) 282, 673, 1507
PscI ACATGT 1 cut(s) 391
PshBI ATTAAT 2 cut(s) 618, 1107
Psp5II RGGWCCY 3 cut(s) 282, 673, 1507
Psp6I CCWGG 5 cut(s) 242, 798, 1549, 1736, 2356
PspGI CCWGG 5 cut(s) 242, 798, 1549, 1736, 2356
PspN4I GGNNCC 9 cut(s) 147, 172, 283, 284, 674, 675, 1096, 1508, 1692
PspPI GGNCC 9 cut(s) 145, 282, 673, 1076, 1507, 1564, 1691, 1901, 2294
PspPPI RGGWCCY 3 cut(s) 282, 673, 1507
PsrI GAACNNNNNNTAC 2 cut(s) 2392, 2424
PstI CTGCAG 1 cut(s) 1306
PstNI CAGNNNCTG 1 cut(s) 518
PsuI RGATCY 4 cut(s) 170, 523, 1553, 2362
PteI GCGCGC 1 cut(s) 715
RsaI GTAC 4 cut(s) 294, 935, 1866, 1930
RsaNI GTAC 4 cut(s) 293, 934, 1865, 1929
RseI CAYNNNNRTG 4 cut(s) 900, 1139, 1710, 2107
SaqAI TTAA 9 cut(s) 618, 648, 840, 924, 939, 1107, 1830, 1938, 2418
SatI GCNGC 8 cut(s) 60, 100, 165, 342, 708, 963, 1982, 1985
Sau96I GGNCC 9 cut(s) 145, 282, 673, 1076, 1507, 1564, 1691, 1901, 2294
SchI GAGTC 1 cut(s) 395
SduI GDGCHC 1 cut(s) 2028
SfaNI GCATC 4 cut(s) 276, 424, 1019, 2358
SfcI CTRYAG 1 cut(s) 1302
Sfr274I CTCGAG 2 cut(s) 762, 2129
SfuI TTCGAA 1 cut(s) 632
SinI GGWCC 7 cut(s) 145, 282, 673, 1076, 1507, 1901, 2294
SlaI CTCGAG 2 cut(s) 762, 2129
SmiMI CAYNNNNRTG 4 cut(s) 900, 1139, 1710, 2107
SmlI CTYRAG 4 cut(s) 762, 1806, 2129, 2313
SmoI CTYRAG 4 cut(s) 762, 1806, 2129, 2313
SphI GCATGC 1 cut(s) 2128
SsiI CCGC 9 cut(s) 99, 143, 164, 167, 223, 753, 1932, 2228, 2297
SspMI CTAG 4 cut(s) 1667, 2046, 2090, 2429
StyI CCWWGG 1 cut(s) 2241
TaaI ACNGT 7 cut(s) 542, 1213, 1355, 1437, 1594, 1653, 1823
TaiI ACGT 3 cut(s) 298, 1005, 1364
TaqI TCGA 8 cut(s) 251, 570, 632, 684, 763, 1455, 2058, 2130
TatI WGTACW 1 cut(s) 933
TauI GCSGC 2 cut(s) 102, 167
TfiI GAWTC 5 cut(s) 580, 1040, 1812, 2086, 2288
Tru1I TTAA 9 cut(s) 618, 648, 840, 924, 939, 1107, 1830, 1938, 2418
Tru9I TTAA 9 cut(s) 618, 648, 840, 924, 939, 1107, 1830, 1938, 2418
TscAI CASTG 2 cut(s) 1075, 1918
TseFI GTSAC 2 cut(s) 902, 1823
TseI GCWGC 6 cut(s) 59, 341, 707, 962, 1981, 1984
Tsp45I GTSAC 2 cut(s) 902, 1823
TspDTI ATGAA 5 cut(s) 26, 821, 1689, 1781, 2001
TspGWI ACGGA 5 cut(s) 109, 349, 827, 976, 1893
TspRI CASTG 2 cut(s) 1075, 1918
VpaK11BI GGWCC 7 cut(s) 145, 282, 673, 1076, 1507, 1901, 2294
VspI ATTAAT 2 cut(s) 618, 1107
XapI RAATTY 5 cut(s) 854, 871, 1574, 1605, 2260
XceI RCATGY 2 cut(s) 395, 2128
XcmI CCANNNNNNNNNTGG 1 cut(s) 1734
XhoI CTCGAG 2 cut(s) 762, 2129
XspI CTAG 4 cut(s) 1667, 2046, 2090, 2429
Zsp2I ATGCAT 1 cut(s) 1763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.