pycom08g01430

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005348
Physical Location & Seq
Reverse (-)
1106146 .. 1107597
1452 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01430.1

Sequence Viewer

Length: 348 bp
ATGGAGGCTTGCAAAAAGGGCTACGCGTGGGCAGTCTCAGCCGGGCTCTGCGCCGCTTCCGCCGCCGTTTCGGCGAAGCTCATCACTAATCAGATTGTAAAGTACTGTTTCGTGGTATTATTCAACGTGACGATGTGGGGGTGCTTTGTGCAAAGCTTAAAAAATCTGTCGTCTCTGCAAGCTACAGCGACGAACTTTGCTACCAACTTCCTCACTTCTGGTCTGGCTGGATACTTTTTGTTTCAAGAACCCTTATCTGAGAAGTGGTTTGCAGGTGCCCTGCTCATTGTCATTGGCGCTCTCGTGCTTAGTAAGTCAAGTATCGAGAAGAATGTTCATACGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

116

Amino Acids

12.35

Weight (kDa)

8.75

Isoelectric Point (pI)

16.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 31 - 104 5e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 263
Acc36I ACCTGC 1 cut(s) 263
AccB1I GGYRCC 1 cut(s) 275
AccII CGCG 1 cut(s) 26
AciI CCGC 3 cut(s) 54, 60, 63
AfaI GTAC 1 cut(s) 104
AflIII ACRYGT 1 cut(s) 24
AgsI TTSAA 2 cut(s) 124, 245
AluBI AGCT 3 cut(s) 79, 156, 182
AluI AGCT 3 cut(s) 79, 156, 182
Alw26I GTCTC 2 cut(s) 40, 177
AspLEI GCGC 2 cut(s) 53, 299
AsuC2I CCSGG 1 cut(s) 43
BaeGI GKGCMC 1 cut(s) 280
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 1 cut(s) 48
BauI CACGAG 1 cut(s) 302
BceAI ACGGC 1 cut(s) 50
BciVI GTATCC 1 cut(s) 224
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 2 cut(s) 40, 177
BfmI CTRYAG 1 cut(s) 183
BfoI RGCGCY 1 cut(s) 300
BfuAI ACCTGC 1 cut(s) 263
BfuI GTATCC 1 cut(s) 224
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 2 cut(s) 54, 63
BlsI GCNGC 2 cut(s) 55, 64
BmcAI AGTACT 1 cut(s) 104
Bme1390I CCNGG 1 cut(s) 43
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 1 cut(s) 43
BpuMI CCSGG 1 cut(s) 43
BseMII CTCAG 2 cut(s) 51, 249
BseSI GKGCMC 1 cut(s) 280
Bsh1236I CGCG 1 cut(s) 26
BshNI GGYRCC 1 cut(s) 275
BsiSI CCGG 1 cut(s) 42
BsmAI GTCTC 2 cut(s) 40, 177
BsmBI CGTCTC 1 cut(s) 177
Bsp1286I GDGCHC 2 cut(s) 48, 280
BspACI CCGC 3 cut(s) 54, 60, 63
BspCNI CTCAG 2 cut(s) 50, 250
BspFNI CGCG 1 cut(s) 26
BspLI GGNNCC 1 cut(s) 277
BspMI ACCTGC 1 cut(s) 263
BspT107I GGYRCC 1 cut(s) 275
BssSI CACGAG 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 107
BstC8I GCNNGC 2 cut(s) 10, 180
BstDEI CTNAG 3 cut(s) 37, 258, 308
BstFNI CGCG 1 cut(s) 26
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 2 cut(s) 53, 299
BstMAI GTCTC 2 cut(s) 40, 177
BstMWI GCNNNNNNNGC 5 cut(s) 18, 38, 59, 62, 71
BstSCI CCNGG 1 cut(s) 41
BstSFI CTRYAG 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 280
BstUI CGCG 1 cut(s) 26
BsuI GTATCC 1 cut(s) 224
BveI ACCTGC 1 cut(s) 263
Cac8I GCNNGC 2 cut(s) 10, 180
CfoI GCGC 2 cut(s) 53, 299
Csp6I GTAC 1 cut(s) 103
CviJI RGCY 8 cut(s) 8, 21, 41, 46, 79, 156, 182, 227
CviKI_1 RGCY 8 cut(s) 8, 21, 41, 46, 79, 156, 182, 227
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 3 cut(s) 37, 258, 308
EciI GGCGGA 1 cut(s) 49
Eco24I GRGCYC 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 48
Esp3I CGTCTC 1 cut(s) 177
FaiI YATR 1 cut(s) 339
Fnu4HI GCNGC 2 cut(s) 54, 63
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 2 cut(s) 54, 63
GlaI GCGC 2 cut(s) 52, 298
GluI GCNGC 2 cut(s) 54, 63
HaeII RGCGCY 1 cut(s) 300
HapII CCGG 1 cut(s) 42
HhaI GCGC 2 cut(s) 53, 299
Hin6I GCGC 2 cut(s) 51, 297
HinP1I GCGC 2 cut(s) 51, 297
HindIII AAGCTT 1 cut(s) 154
HpaII CCGG 1 cut(s) 42
Hpy188I TCNGA 2 cut(s) 93, 259
Hpy188III TCNNGA 2 cut(s) 245, 325
Hpy99I CGWCG 1 cut(s) 193
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 4 cut(s) 12, 151, 178, 272
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 38, 59, 62, 71
HpyF3I CTNAG 3 cut(s) 37, 258, 308
HpySE526I ACGT 1 cut(s) 126
HspAI GCGC 2 cut(s) 51, 297
LpnPI CCDG 6 cut(s) 55, 204, 209, 213, 258, 293
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 127
MboII GAAGA 1 cut(s) 340
MhlI GDGCHC 2 cut(s) 48, 280
MluI ACGCGT 1 cut(s) 24
MnlI CCTC 1 cut(s) 221
MseI TTAA 2 cut(s) 158, 346
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 1 cut(s) 43
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 5 cut(s) 18, 38, 59, 62, 71
NciI CCSGG 1 cut(s) 43
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 127
PaqCI CACCTGC 1 cut(s) 263
PkrI GCNGC 2 cut(s) 55, 64
PspN4I GGNNCC 1 cut(s) 277
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
SaqAI TTAA 2 cut(s) 158, 346
SatI GCNGC 2 cut(s) 54, 63
ScaI AGTACT 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 43
SduI GDGCHC 2 cut(s) 48, 280
SetI ASST 5 cut(s) 81, 129, 158, 184, 277
SfcI CTRYAG 1 cut(s) 183
SsiI CCGC 3 cut(s) 54, 60, 63
StyD4I CCNGG 1 cut(s) 41
TaaI ACNGT 1 cut(s) 107
TaiI ACGT 1 cut(s) 129
TaqI TCGA 1 cut(s) 324
TatI WGTACW 1 cut(s) 102
TauI GCSGC 2 cut(s) 56, 65
Tru1I TTAA 2 cut(s) 158, 346
Tru9I TTAA 2 cut(s) 158, 346
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 326
ZrmI AGTACT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.