pycom08g01840

beta-galactosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009802
Physical Location & Seq
Reverse (-)
1354629 .. 1360486
5858 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2337 bp
ATGGAAACTCACTCGGTTTCCAAGCTCTTAGTTTTGTTCTTGACGATGACATTGTTCCTGGCTTCCAAGCTGATCCACTGCACCACCGTCACCTACGACAAGAAGGCCATTCTCATCAATGGCCAAAGAAGATTGCTCATTTCTGGATCCATTCACTATCCCAGAAGTACCCCTGAAATGTGGGAAGGCCTCATACAGAAAGCCAAAGATGGAGGCTTGGATGTGATCGACACCTACGTTTTCTGGAACGGCCATGAACCTTCTCCGGGCAACTGCTTAAATTTTGACTCCCATTTGTGCAGAGGATTTCCTGTTTGGCTTAAATATGTACCTGGCATCAGTTTCAGAACAGATAATGGGCCATTTAAGGTGGCAATGCAAGGGTTTACCCAGAAGATTGTCCAGATGATGAAAAATGAGAAGCTATTTGCATCACAAGGTGGTCCTATCATCCTTTCCCAGATTGAGAATGAGTATGGACCGGAGCGGAAGGCACTTGGAGCTGCTGGACAGAACTACATTAACTGGGCTGCTAAAATGGCAGTTGGATTGGATACGGGAGTCCCATGGGTGATGTGCAAGGAAGATGATGCCCCGGACCCTATGATAAATGCATGCAACGGTTTTTACTGCGATGGATTCACTCCCAACAAACCTTACAAGCCTACCATGTGGACGGAAGCTTGGAGCGGCTGGTTTACGGAGTTTGGTGGCACAATTCACCATCGACCTGTTCAAGATTTAGCATTTGCAGTTGCTCGATTCATAGAAAGGGGCGGCTCATATGTGAATTACTACATGTATCATGGAGGAACAAACTTCGGACGCACTGCTGGAGGCCCCTTCATTACAACGAGTTACGACTATGATGCTCCCATTGATGAATACGGACCGAGAAGATGCGCAGCATTTCTCTCAAACTTCCATTCAGTAGAAGCAAGGGTGACTTTTAACAACAAGCACTACGATTTGCCTCCTTGGTCTGTTAGCATCCTTCCTGATTGCAGGAATGAAGTATATAACACTGCAAAGGTGGGAGTTCAAACGTCACACGTGCAGATGATCCCAACGAACAGTCGGTTGTTTTCATGGCAGACTTACGATGAAGATATCTCTTCTGTACACGAGAGATCGAGCATACCAGCTATCGGACTTCTTGAGCAGATAAATGTTACTAAAGATACTAGTGACTATCTGTGGTACATGACGAATGTTGACATCAGTTCTTCAGACCTCAGCGGAGGAAAAAAGCCTACTCTTACTGTACAGTCAGCTGGCCATGCCCTCCATGTCTTCGTCAACGGACAGTTTTCAGGGTCGGCGTTTGGAACTAGGGAGCAGAGGCAATTTACATTTGCTGAACCAGTCAACCTGCACGCTGGAATAAACAGAATTGCACTACTCAGCATAGCTGTTGGATTACCGAATGTCGGGTTGCATTACGAGTCATGGAAAACAGGAATCCAAGGTCCGGTTTTTCTGGATGGTCTTGGCAACGGAAAGAAAGACTTAACATTGCATAAGTGGTTCAACAAGGTCGGCCTTAAGGGAGAAGCAATGAATTTGGTCTCTCCAAACGGAGCATCATCGGTTGGTTGGATTCGAAGGTCACTAGCTACTCAAACCAAGCAGACGTTGAAATGGTACAAGGCTTATTTCAATGCACCCGGAGGAAATGAGCCGTTGGCATTGGACATGAGGAGGATGGGAAAGGGACAAGTATGGATCGATGGGCAGAGTATCGGTAGATACTGGATGGCTTATGCCAAAGGTGACTGCAGTTCGTGTAGTTACATTGGGACATTCCGACCCACAAAGTGTCAACTTCACTGTGGCCGACCAACTCAACGATGGTATCATGTTCCTCGTTCATGGTTAAAGCCAACGCAAAATTTGGTGGTAGTGTTTGAGGAACTAGGCGGAGATCGATCAAAGATAACCCTAGTGAGGAGATCAGTGACCGGTGTATGTGGTGACTTACATGAGAACCACCCGAACGCTGAAAATTTTGATGTCGATGGTAATGAAGATTCTAAAACGCTTCACCAGGCTCAGGTTCATCTGCATTGTGCACCAGGGCAGTCTATCTCGAGCATTAAGTTTGCAAGTTTCGGAACTCCTAGCGGAACTTGTGGGAGTTTCCAGCAAGGAACATGTCACGCGACCAACTCACACGCTGTTGTGGAGAAGAATTGTATCGGCCGCGAGAGTTGCTCAGTTGCTGTATCGAATAGCACCTTCGAGACAGATCCATGTCCGAACGTACTCAAGCGGCTGTCTGTTGAAGCTGTTTGTTCGACAGAAGTTACGACCGATGAAGCCAATTCGAGGAGATGA

Protein Analysis

779

Amino Acids

86.32

Weight (kDa)

8.25

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_35 PF01301 37 - 95 9.8e-21 Glycosyl hydrolases family 35
Glyco_hydro_35 PF01301 102 - 299 5e-64 Glycosyl hydrolases family 35
GHD PF17834 300 - 345 8e-21 Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd PF21467 398 - 463 2e-09 Beta-galactosidase, galactose-binding domain
BetaGal_gal-bd PF21467 547 - 631 2.8e-25 Beta-galactosidase, galactose-binding domain
SUEL_Lectin PF02140 688 - 765 3e-23 D-galactoside/L-rhamnose binding SUEL lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45130 AT1G45130
fragaria_vesca FvH4_2g39200 FvH4_2g39200
malus_domestica MD08G1023600.v1.1 MD15G1021100.v1.1
prunus_persica Prupe.1G372800_v2.0.a1
pyrus_communis pycom08g01840 pycom15g01740
rosa_chinensis RchiOBHm_Chr6g0301851
rosa_laevigata RLG00000011216 RLG00000011219
rosa_multiflora Rmu_sc0000407.1_g000009
rosa_roxburghii Rroxscaffold_7G00166340
rosa_rugosa Rorug06G0310300 Rorug06G0310400 Rorug06G0310500
rosa_samantha Rh6BG428900 Rh6CG435500 Rh6DG422300
rosa_wichuraiana Rw6G036570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 904
Acc36I ACCTGC 1 cut(s) 1380
AccBSI CCGCTC 2 cut(s) 487, 690
AccII CGCG 2 cut(s) 2162, 2205
AciI CCGC 8 cut(s) 487, 690, 777, 1239, 1920, 2124, 2203, 2272
AclWI GGATC 6 cut(s) 67, 141, 154, 1057, 1733, 2243
AcoI YGGCCR 5 cut(s) 121, 250, 1276, 1834, 2200
AcsI RAATTY 4 cut(s) 280, 1561, 1891, 2005
AcuI CTGAAG 1 cut(s) 1212
AcvI CACGTG 1 cut(s) 1054
AdeI CACNNNGTG 2 cut(s) 440, 1818
AfaI GTAC 7 cut(s) 169, 330, 1122, 1202, 1266, 1646, 2265
AfiI CCNNNNNNNGG 7 cut(s) 266, 1148, 1430, 1471, 1947, 2053, 2328
AflII CTTAAG 1 cut(s) 1544
AflIII ACRYGT 3 cut(s) 798, 1051, 2153
AgeI ACCGGT 1 cut(s) 1961
AgsI TTSAA 6 cut(s) 737, 1043, 1531, 1639, 1660, 2285
AhlI ACTAGT 1 cut(s) 1184
AjnI CCWGG 4 cut(s) 57, 331, 2046, 2074
Alw21I GWGCWC 1 cut(s) 2074
Alw26I GTCTC 2 cut(s) 1573, 2237
Alw44I GTGCAC 1 cut(s) 2070
AlwI GGATC 6 cut(s) 67, 141, 154, 1057, 1733, 2243
AlwNI CAGNNNCTG 1 cut(s) 2222
Ama87I CYCGRG 1 cut(s) 2089
ApaLI GTGCAC 1 cut(s) 2070
ApeKI GCWGC 3 cut(s) 503, 530, 905
ApoI RAATTY 4 cut(s) 280, 1561, 1891, 2005
AsiGI ACCGGT 1 cut(s) 1961
AspLEI GCGC 1 cut(s) 905
AspS9I GGNCC 7 cut(s) 359, 443, 479, 598, 839, 890, 1469
AsuC2I CCSGG 3 cut(s) 267, 596, 1668
AsuHPI GGTGA 7 cut(s) 82, 583, 713, 955, 1784, 1985, 2036
AsuII TTCGAA 1 cut(s) 1603
AvaI CYCGRG 1 cut(s) 2089
AvaII GGWCC 5 cut(s) 443, 479, 598, 890, 1469
BaeGI GKGCMC 1 cut(s) 2074
BaeI ACNNNNGTAYC 4 cut(s) 546, 579, 1838, 1871
BalI TGGCCA 2 cut(s) 123, 1278
BamHI GGATCC 1 cut(s) 146
BarI GAAGNNNNNNTAC 2 cut(s) 177, 209
BauI CACGAG 1 cut(s) 1124
BbrPI CACGTG 1 cut(s) 1054
BbsI GAAGAC 1 cut(s) 1285
Bbv12I GWGCWC 1 cut(s) 2074
BbvCI CCTCAGC 1 cut(s) 1235
BbvI GCAGC 3 cut(s) 490, 517, 917
BccI CCATC 9 cut(s) 203, 629, 732, 1478, 1699, 1724, 1750, 1845, 2012
BceAI ACGGC 2 cut(s) 265, 1666
BcgI CGANNNNNNTGC 2 cut(s) 851, 885
BciT130I CCWGG 4 cut(s) 59, 333, 2048, 2076
BciVI GTATCC 1 cut(s) 547
BcnI CCSGG 3 cut(s) 267, 596, 1668
BcoDI GTCTC 2 cut(s) 1573, 2237
BcuI ACTAGT 1 cut(s) 1184
BfaI CTAG 6 cut(s) 1185, 1332, 1613, 1916, 1943, 2121
BfmI CTRYAG 1 cut(s) 1777
BfrI CTTAAG 1 cut(s) 1544
BfuAI ACCTGC 1 cut(s) 1380
BfuI GTATCC 1 cut(s) 547
BisI GCNGC 7 cut(s) 504, 531, 691, 778, 906, 2203, 2273
BlsI GCNGC 7 cut(s) 505, 532, 692, 779, 907, 2204, 2274
Bme1390I CCNGG 7 cut(s) 59, 267, 333, 596, 1668, 2048, 2076
Bme18I GGWCC 5 cut(s) 443, 479, 598, 890, 1469
BmeT110I CYCGRG 1 cut(s) 2089
BmgT120I GGNCC 7 cut(s) 359, 443, 479, 598, 839, 890, 1469
BmiI GGNNCC 3 cut(s) 148, 600, 841
BmrFI CCNGG 7 cut(s) 59, 267, 333, 596, 1668, 2048, 2076
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 7 cut(s) 345, 440, 580, 859, 890, 999, 1592
BmuI ACTGGG 1 cut(s) 535
BpiI GAAGAC 1 cut(s) 1285
BplI GAGNNNNNCTC 2 cut(s) 2198, 2230
BpmI CTGGAG 1 cut(s) 855
Bpu10I CCTNAGC 2 cut(s) 1235, 2052
Bpu14I TTCGAA 1 cut(s) 1603
BpuEI CTTGAG 2 cut(s) 1178, 2252
BpuMI CCSGG 3 cut(s) 267, 596, 1668
Bsa29I ATCGAT 2 cut(s) 1728, 1927
BsaAI YACGTR 1 cut(s) 1054
BsaI GGTCTC 1 cut(s) 1573
BsaJI CCNNGG 5 cut(s) 566, 594, 977, 1465, 2075
BsaWI WCCGGW 3 cut(s) 481, 1471, 1961
Bsc4I CCNNNNNNNGG 7 cut(s) 266, 1148, 1430, 1471, 1947, 2053, 2328
Bse118I RCCGGY 1 cut(s) 1961
Bse1I ACTGG 3 cut(s) 530, 1364, 1757
Bse3DI GCAATG 3 cut(s) 381, 1514, 1563
BseBI CCWGG 4 cut(s) 59, 333, 2048, 2076
BseCI ATCGAT 2 cut(s) 1728, 1927
BseDI CCNNGG 5 cut(s) 566, 594, 977, 1465, 2075
BseGI GGATG 6 cut(s) 226, 450, 990, 1489, 1710, 1761
BseLI CCNNNNNNNGG 7 cut(s) 266, 1148, 1430, 1471, 1947, 2053, 2328
BseMI GCAATG 3 cut(s) 381, 1514, 1563
BseMII CTCAG 4 cut(s) 1249, 1417, 2066, 2229
BseNI ACTGG 3 cut(s) 530, 1364, 1757
BseRI GAGGAG 2 cut(s) 1714, 1963
BseSI GKGCMC 1 cut(s) 2074
BseX3I CGGCCG 1 cut(s) 2200
BseXI GCAGC 3 cut(s) 490, 517, 917
BsgI GTGCAG 4 cut(s) 64, 319, 1076, 1358
Bsh1236I CGCG 2 cut(s) 2162, 2205
Bsh1285I CGRYCG 2 cut(s) 2203, 2313
BshTI ACCGGT 1 cut(s) 1961
BshVI ATCGAT 2 cut(s) 1728, 1927
BsiEI CGRYCG 2 cut(s) 2203, 2313
BsiHKAI GWGCWC 1 cut(s) 2074
BsiHKCI CYCGRG 1 cut(s) 2089
BsiSI CCGG 6 cut(s) 266, 482, 596, 1472, 1668, 1962
BslFI GGGAC 3 cut(s) 548, 1728, 1813
BslI CCNNNNNNNGG 7 cut(s) 266, 1148, 1430, 1471, 1947, 2053, 2328
BsmAI GTCTC 2 cut(s) 1573, 2237
BsmFI GGGAC 3 cut(s) 548, 1728, 1813
Bso31I GGTCTC 1 cut(s) 1573
BsoBI CYCGRG 1 cut(s) 2089
Bsp119I TTCGAA 1 cut(s) 1603
Bsp1286I GDGCHC 1 cut(s) 2074
Bsp1407I TGTACA 2 cut(s) 1120, 1264
Bsp19I CCATGG 1 cut(s) 566
BspACI CCGC 8 cut(s) 487, 690, 777, 1239, 1920, 2124, 2203, 2272
BspCNI CTCAG 4 cut(s) 1248, 1416, 2065, 2228
BspDI ATCGAT 2 cut(s) 1728, 1927
BspFNI CGCG 2 cut(s) 2162, 2205
BspLI GGNNCC 3 cut(s) 148, 600, 841
BspMAI CTGCAG 1 cut(s) 1781
BspMI ACCTGC 1 cut(s) 1380
BspPI GGATC 6 cut(s) 67, 141, 154, 1057, 1733, 2243
BspT104I TTCGAA 1 cut(s) 1603
BspTI CTTAAG 1 cut(s) 1544
BspTNI GGTCTC 1 cut(s) 1573
BsrBI CCGCTC 2 cut(s) 487, 690
BsrDI GCAATG 3 cut(s) 381, 1514, 1563
BsrFI RCCGGY 1 cut(s) 1961
BsrGI TGTACA 2 cut(s) 1120, 1264
BsrI ACTGG 3 cut(s) 530, 1364, 1757
BssAI RCCGGY 1 cut(s) 1961
BssECI CCNNGG 5 cut(s) 566, 594, 977, 1465, 2075
BssSI CACGAG 1 cut(s) 1124
BssT1I CCWWGG 3 cut(s) 566, 977, 1465
Bst2BI CACGAG 1 cut(s) 1124
Bst2UI CCWGG 4 cut(s) 59, 333, 2048, 2076
Bst4CI ACNGT 7 cut(s) 88, 623, 1076, 1264, 1269, 1308, 1832
Bst6I CTCTTC 1 cut(s) 1120
BstAFI CTTAAG 1 cut(s) 1544
BstAUI TGTACA 2 cut(s) 1120, 1264
BstBAI YACGTR 1 cut(s) 1054
BstBI TTCGAA 1 cut(s) 1603
BstC8I GCNNGC 3 cut(s) 616, 1276, 1377
BstDEI CTNAG 5 cut(s) 28, 1235, 1403, 2052, 2215
BstDSI CCRYGG 1 cut(s) 566
BstF5I GGATG 6 cut(s) 226, 450, 990, 1489, 1710, 1761
BstFNI CGCG 2 cut(s) 2162, 2205
BstHHI GCGC 1 cut(s) 905
BstMAI GTCTC 2 cut(s) 1573, 2237
BstMCI CGRYCG 2 cut(s) 2203, 2313
BstMWI GCNNNNNNNGC 4 cut(s) 500, 539, 1280, 2211
BstNI CCWGG 4 cut(s) 59, 333, 2048, 2076
BstNSI RCATGY 3 cut(s) 618, 802, 2157
BstSCI CCNGG 7 cut(s) 57, 265, 331, 594, 1666, 2046, 2074
BstSFI CTRYAG 1 cut(s) 1777
BstSLI GKGCMC 1 cut(s) 2074
BstUI CGCG 2 cut(s) 2162, 2205
BstV1I GCAGC 3 cut(s) 490, 517, 917
BstV2I GAAGAC 1 cut(s) 1285
BstX2I RGATCY 2 cut(s) 146, 2248
BstYI RGATCY 2 cut(s) 146, 2248
BstZI CGGCCG 1 cut(s) 2200
Bsu15I ATCGAT 2 cut(s) 1728, 1927
BsuI GTATCC 1 cut(s) 547
BsuTUI ATCGAT 2 cut(s) 1728, 1927
BtgI CCRYGG 1 cut(s) 566
BtgZI GCGATG 1 cut(s) 648
BtsCI GGATG 6 cut(s) 226, 450, 990, 1489, 1710, 1761
BtsI GCAGTG 3 cut(s) 76, 828, 1023
BtsIMutI CAGTG 5 cut(s) 76, 828, 1023, 1828, 1962
BveI ACCTGC 1 cut(s) 1380
Cac8I GCNNGC 3 cut(s) 616, 1276, 1377
CaiI CAGNNNCTG 1 cut(s) 2222
CfoI GCGC 1 cut(s) 905
Cfr10I RCCGGY 1 cut(s) 1961
Cfr13I GGNCC 7 cut(s) 359, 443, 479, 598, 839, 890, 1469
ClaI ATCGAT 2 cut(s) 1728, 1927
CpoI CGGWCCG 1 cut(s) 890
CseI GACGC 1 cut(s) 834
Csp6I GTAC 7 cut(s) 168, 329, 1121, 1201, 1265, 1645, 2264
CspAI ACCGGT 1 cut(s) 1961
CspI CGGWCCG 1 cut(s) 890
CviQI GTAC 7 cut(s) 168, 329, 1121, 1201, 1265, 1645, 2264
DdeI CTNAG 5 cut(s) 28, 1235, 1403, 2052, 2215
DraIII CACNNNGTG 2 cut(s) 440, 1818
EaeI YGGCCR 5 cut(s) 121, 250, 1276, 1834, 2200
EagI CGGCCG 1 cut(s) 2200
Eam1104I CTCTTC 1 cut(s) 1120
EarI CTCTTC 1 cut(s) 1120
EciI GGCGGA 1 cut(s) 1935
EclXI CGGCCG 1 cut(s) 2200
Eco130I CCWWGG 3 cut(s) 566, 977, 1465
Eco147I AGGCCT 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 1573
Eco32I GATATC 1 cut(s) 1111
Eco47I GGWCC 5 cut(s) 443, 479, 598, 890, 1469
Eco52I CGGCCG 1 cut(s) 2200
Eco57I CTGAAG 1 cut(s) 1212
Eco72I CACGTG 1 cut(s) 1054
Eco88I CYCGRG 1 cut(s) 2089
EcoO109I RGGNCCY 1 cut(s) 839
EcoRII CCWGG 4 cut(s) 57, 331, 2046, 2074
EcoRV GATATC 1 cut(s) 1111
EcoT14I CCWWGG 3 cut(s) 566, 977, 1465
EcoT22I ATGCAT 1 cut(s) 616
ErhI CCWWGG 3 cut(s) 566, 977, 1465
FalI AAGNNNNNCTT 4 cut(s) 931, 963, 1493, 1525
FaqI GGGAC 3 cut(s) 548, 1728, 1813
FauNDI CATATG 1 cut(s) 784
Fnu4HI GCNGC 7 cut(s) 504, 531, 691, 778, 906, 2203, 2273
FokI GGATG 6 cut(s) 233, 437, 977, 1496, 1717, 1768
Fsp4HI GCNGC 7 cut(s) 504, 531, 691, 778, 906, 2203, 2273
FspBI CTAG 6 cut(s) 1185, 1332, 1613, 1916, 1943, 2121
FspI TGCGCA 1 cut(s) 904
GlaI GCGC 1 cut(s) 904
GluI GCNGC 7 cut(s) 504, 531, 691, 778, 906, 2203, 2273
GsuI CTGGAG 1 cut(s) 855
HapII CCGG 6 cut(s) 266, 482, 596, 1472, 1668, 1962
HgaI GACGC 1 cut(s) 834
HhaI GCGC 1 cut(s) 905
Hin6I GCGC 1 cut(s) 903
HinP1I GCGC 1 cut(s) 903
HincII GTYRAC 4 cut(s) 1216, 1300, 1369, 1823
HindII GTYRAC 4 cut(s) 1216, 1300, 1369, 1823
HindIII AAGCTT 1 cut(s) 681
HinfI GANTC 8 cut(s) 287, 561, 639, 762, 1445, 1461, 1600, 2030
HpaII CCGG 6 cut(s) 266, 482, 596, 1472, 1668, 1962
HphI GGTGA 7 cut(s) 82, 583, 713, 955, 1784, 1985, 2036
Hpy166II GTNNAC 9 cut(s) 387, 675, 699, 1123, 1216, 1300, 1369, 1823, 2072
Hpy188I TCNGA 7 cut(s) 347, 824, 1151, 1231, 1808, 2114, 2259
Hpy8I GTNNAC 9 cut(s) 387, 675, 699, 1123, 1216, 1300, 1369, 1823, 2072
HpyAV CCTTC 8 cut(s) 97, 179, 270, 484, 853, 1004, 1599, 2248
HpyCH4III ACNGT 7 cut(s) 88, 623, 1076, 1264, 1269, 1308, 1832
HpyCH4IV ACGT 5 cut(s) 237, 1046, 1053, 1634, 2262
HpyF10VI GCNNNNNNNGC 4 cut(s) 500, 539, 1280, 2211
HpyF3I CTNAG 5 cut(s) 28, 1235, 1403, 2052, 2215
HpySE526I ACGT 5 cut(s) 237, 1046, 1053, 1634, 2262
HspAI GCGC 1 cut(s) 903
LmnI GCTCC 6 cut(s) 484, 500, 687, 877, 1336, 1580
Lsp1109I GCAGC 3 cut(s) 490, 517, 917
LweI GCATC 7 cut(s) 345, 440, 580, 859, 890, 999, 1592
MaeI CTAG 6 cut(s) 1185, 1332, 1613, 1916, 1943, 2121
MaeII ACGT 5 cut(s) 237, 1046, 1053, 1634, 2262
MbiI CCGCTC 2 cut(s) 487, 690
MflI RGATCY 2 cut(s) 146, 2248
MhlI GDGCHC 1 cut(s) 2074
MlsI TGGCCA 2 cut(s) 123, 1278
MluNI TGGCCA 2 cut(s) 123, 1278
MlyI GAGTC 3 cut(s) 281, 570, 1454
MmeI TCCRAC 4 cut(s) 526, 1396, 1577, 1831
Mox20I TGGCCA 2 cut(s) 123, 1278
Mph1103I ATGCAT 1 cut(s) 616
MscI TGGCCA 2 cut(s) 123, 1278
MseI TTAA 9 cut(s) 278, 321, 366, 522, 951, 1511, 1545, 1877, 2097
Msp20I TGGCCA 2 cut(s) 123, 1278
MspA1I CMGCKG 2 cut(s) 1239, 1274
MspCI CTTAAG 1 cut(s) 1544
MspI CCGG 6 cut(s) 266, 482, 596, 1472, 1668, 1962
MspR9I CCNGG 7 cut(s) 59, 267, 333, 596, 1668, 2048, 2076
MvaI CCWGG 4 cut(s) 59, 333, 2048, 2076
MvnI CGCG 2 cut(s) 2162, 2205
MwoI GCNNNNNNNGC 4 cut(s) 500, 539, 1280, 2211
NciI CCSGG 3 cut(s) 267, 596, 1668
NcoI CCATGG 1 cut(s) 566
NdeI CATATG 1 cut(s) 784
NlaIV GGNNCC 3 cut(s) 148, 600, 841
NmuCI GTSAC 9 cut(s) 88, 943, 1047, 1187, 1608, 1772, 1957, 1973, 2156
NsbI TGCGCA 1 cut(s) 904
NsiI ATGCAT 1 cut(s) 616
NspI RCATGY 3 cut(s) 618, 802, 2157
NspV TTCGAA 1 cut(s) 1603
PaeI GCATGC 1 cut(s) 618
PaeR7I CTCGAG 1 cut(s) 2089
PceI AGGCCT 1 cut(s) 189
PciI ACATGT 2 cut(s) 798, 2153
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PfeI GAWTC 5 cut(s) 639, 762, 1461, 1600, 2030
PinAI ACCGGT 1 cut(s) 1961
PkrI GCNGC 7 cut(s) 505, 532, 692, 779, 907, 2204, 2274
PleI GAGTC 3 cut(s) 281, 569, 1453
PmaCI CACGTG 1 cut(s) 1054
PmlI CACGTG 1 cut(s) 1054
PpsI GAGTC 3 cut(s) 281, 569, 1453
Ppu21I YACGTR 1 cut(s) 1054
PscI ACATGT 2 cut(s) 798, 2153
Psp6I CCWGG 4 cut(s) 57, 331, 2046, 2074
PspCI CACGTG 1 cut(s) 1054
PspGI CCWGG 4 cut(s) 57, 331, 2046, 2074
PspN4I GGNNCC 3 cut(s) 148, 600, 841
PspPI GGNCC 7 cut(s) 359, 443, 479, 598, 839, 890, 1469
PstI CTGCAG 1 cut(s) 1781
PstNI CAGNNNCTG 1 cut(s) 2222
PsuI RGATCY 2 cut(s) 146, 2248
PvuII CAGCTG 1 cut(s) 1274
RsaI GTAC 7 cut(s) 169, 330, 1122, 1202, 1266, 1646, 2265
RsaNI GTAC 7 cut(s) 168, 329, 1121, 1201, 1265, 1645, 2264
Rsr2I CGGWCCG 1 cut(s) 890
RsrII CGGWCCG 1 cut(s) 890
SaqAI TTAA 9 cut(s) 278, 321, 366, 522, 951, 1511, 1545, 1877, 2097
SatI GCNGC 7 cut(s) 504, 531, 691, 778, 906, 2203, 2273
Sau96I GGNCC 7 cut(s) 359, 443, 479, 598, 839, 890, 1469
SchI GAGTC 3 cut(s) 281, 570, 1454
ScrFI CCNGG 7 cut(s) 59, 267, 333, 596, 1668, 2048, 2076
SduI GDGCHC 1 cut(s) 2074
SfaNI GCATC 7 cut(s) 345, 440, 580, 859, 890, 999, 1592
SfcI CTRYAG 1 cut(s) 1777
Sfr274I CTCGAG 1 cut(s) 2089
SfuI TTCGAA 1 cut(s) 1603
SinI GGWCC 5 cut(s) 443, 479, 598, 890, 1469
SlaI CTCGAG 1 cut(s) 2089
SmlI CTYRAG 4 cut(s) 1157, 1544, 2089, 2267
SmoI CTYRAG 4 cut(s) 1157, 1544, 2089, 2267
SpeI ACTAGT 1 cut(s) 1184
SphI GCATGC 1 cut(s) 618
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 8 cut(s) 487, 690, 777, 1239, 1920, 2124, 2203, 2272
SspMI CTAG 6 cut(s) 1185, 1332, 1613, 1916, 1943, 2121
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 7 cut(s) 57, 265, 331, 594, 1666, 2046, 2074
StyI CCWWGG 3 cut(s) 566, 977, 1465
TaaI ACNGT 7 cut(s) 88, 623, 1076, 1264, 1269, 1308, 1832
TaiI ACGT 5 cut(s) 240, 1049, 1056, 1637, 2265
TaqII GACCGA 2 cut(s) 907, 2327
TatI WGTACW 2 cut(s) 1120, 1264
TauI GCSGC 4 cut(s) 693, 780, 2205, 2275
TfiI GAWTC 5 cut(s) 639, 762, 1461, 1600, 2030
Tru1I TTAA 9 cut(s) 278, 321, 366, 522, 951, 1511, 1545, 1877, 2097
Tru9I TTAA 9 cut(s) 278, 321, 366, 522, 951, 1511, 1545, 1877, 2097
TscAI CASTG 5 cut(s) 83, 835, 1030, 1835, 1962
TseFI GTSAC 9 cut(s) 88, 943, 1047, 1187, 1608, 1772, 1957, 1973, 2156
TseI GCWGC 3 cut(s) 503, 530, 905
Tsp45I GTSAC 9 cut(s) 88, 943, 1047, 1187, 1608, 1772, 1957, 1973, 2156
TspGWI ACGGA 6 cut(s) 692, 716, 903, 1317, 1512, 1593
TspRI CASTG 5 cut(s) 83, 835, 1030, 1835, 1962
Vha464I CTTAAG 1 cut(s) 1544
VneI GTGCAC 1 cut(s) 2070
VpaK11BI GGWCC 5 cut(s) 443, 479, 598, 890, 1469
XapI RAATTY 4 cut(s) 280, 1561, 1891, 2005
XceI RCATGY 3 cut(s) 618, 802, 2157
XcmI CCANNNNNNNNNTGG 1 cut(s) 1848
XhoI CTCGAG 1 cut(s) 2089
XspI CTAG 6 cut(s) 1185, 1332, 1613, 1916, 1943, 2121
Zsp2I ATGCAT 1 cut(s) 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.