pycom08g03010

Exocyst complex component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0006651
Physical Location & Seq
Forward (+)
2364636 .. 2373129
8494 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2658 bp
ATGGCGAAATCCAGCGCAGACGACCAGGAGCTTCGGCGGGCCTGCGAGGCCGCCATCGAAGGCACCAAGCAGAGCGTCGTCATGTCGATCCGCGTGGCCAAGAGCCGTGCGATCCGGGGTATAACGCACATGCTCGGCCGCGACATGGCCAAGGCTAGGGTTCTGGCTCTTTCCGTAAAATCCAAGGGCCAGAGGACTAAAGCCTTTCTTCGAGTCTTAAAATATTCCACCGGAGGAGTCCTTGAGCCTGCAAAGTTATACAAACTAAAGCATCTTTCAAAGGTGGAGGTTTTAACGAACGACCCTAGTGGGTGTACTTTTACCCTGGGCTTTGATAATCTTAGGAGTCAAAGTGTTGCTCCTCCTCAATGGACCATGCGAAATATTGATGACAGGAACCGCCTTTTACTTTGCATTTTGAACATATGCAAAGATGCTCTGGGTCAGCTTCCTAAAGTTGTTGGTATAGATGTCGTGGAGATGGCTCTTTGGGCTAAGGAAAATACACCAGCTGTCACTAGCCAAGGAAAGGAGCAAGAGGGACCTGCTGCATCTACAGTAACGGAAAGTGACTTGAAAGTAACTGTTGAAAAAGAACTTGTCTCCCAAGCTGAGGAAGAGGACATGGAGGCACTTTTGGGAACGTATGTACTTTATGTCATGGGTATTGGTGAAGCAGAGGCATTTTCTGAAAGGTTGAAGAGAGAGCTTCTGGCTTTGGAAGCAGCAAATGTGCATGCCATTTTGGAAAGTGAACCCTTGATCGACGAGGTATTGCAAGGCCTTGAGGCAGCAACAAATTGTGTCGATGACATGGACGAATGGTTAGGCATTTTCAATGTGAAACTCAGACACATGAGAGAAGACATCGAATCGATAGAAACCCGCAATAACAAGTTGGAGGTGCAATCTGTAAATAATAGAGCACTCATTGAGGAACTCGATAAGCTTCTTCTAGGTTTGCGCGTTCCTTCTGAGTATGCCGCATGTTTGACGGGAGGTTCATTTGATGAAGCACGTATGCTTCAAAACATAGAAGCGTGTGAGTGGTTGGCTGGTGCTTTACGCAGTCTTGAAGCGCCAAACTTGGACCCTATATATGCAAATATACGGGCTGTTAAAGAGAAGCGAGAAGAACTTGAAAAATTGAAATCTACATTTGTCAGGAAAGCTTCTGAGTTCTTGCGAAATTATTTTGCTAGTTTGGTGGATTTTATGATAAGTGACAAGAGCTACTTTTCTCAGCGGGGCCAACTGAAAAGGCCTGATCATGCTGATCTACGGTACAAATGCAGAACATATGCTCGCCTTCTGCAACATTTAAAGAGTCTTGATAAGAATTGCATGGGCGCTTTGAGGAAAGCATATTGTAGCTCCCTCAACTTGCTTATTCGTCGTGAGGCTCGTGAATTTGCAAATGAGCTTCGTGCTAGTACAAAAGCATCAAGAAATCCAACCGTCTGGCTTGAAACTTCTACTGGGTCTGGTCAAAATGCGAATGCTGCAGACACTTCTACCGTTTCTGAAGCTTATTCCAAGATGCTTACAATTTTTATCCCACTCCTTGTAGATGAGAGTTCTTTCTTTGCGCACTTTATGTGTTTTGAAGTTCCAGCACTTAATCCTCCTGGGGGTACTGCTAATGGAGATAAGTCTGATGACACCAATGACGATGATTTGGGAATCATGGACATTGATGACAAGGACAGCAAGGCTGGTAAAAGTTCAGGAGAGCTTGCAGCATTAAATGAATCTCTTCAGGATTTACTCGATGGAATCCAGGAAGACTTTTATGCCGTTGTGGATTGGGCTTACAAGATTGATCCCTTACGCTGCATATCAATGCATGGGATTACAGAGCGCTATCTTTCTGGTCAGAAAGCTGATGCAGCAGGATTTGTGCGTCTCTTGCTTGGTGATCTGGAGTCGAGGGTTTCTATGCAGTTCAGTCGCTTTGTTGATGAAGCTTGCCACCAGATTGAAAAAAACGAGCGCAATGTTAGACAAATGGGTGTCTTATCATACATCCCAAGATTTGCTACTTTGGCAACACGAATGGAGCAGTACATCCAGGGACAATCTAGGGATTTGGTTGATCAAGCATACACAAAATTTGTTAGCATAATGTTTGTGACTTTGGAGAAAATTGCACAAACAGAGCCAAAATATTCTGATCTTTTTCTATTAGAGAACTATGCTGCATTTCAGAATAGTTTGTATGACCTAGCAAATGTTGTGCCCACCTTGGCCAAATTTTATCACCAGGCAAGTGAAGCTTACGAACAAGCTTGCACACGCCATAACAGCATGATCATATACACTCAATTTGAACGGCTTTTCCAGTTTGCTCGAAAAATAGAGGATTTGATGTTTACAATTGCACCAGAAGAGATCCCTTTCCAGCTTGGGTTGTCAAAGGCGGATCTGCGGAAGGTGTTAAAATCAAGCTTATACGGGCTTGACAAGTCCATCACTGCAATGTACAAGAAGTTGCAGAAAAACATGACTTCAGAAGAACTGCTGCCTTCTTTATGGGAAAAATGCAAGAAGGAATTTCTTGAGAAGTATGAAACTTTTGCTCAACTCGTCGCCAAGATTTACCCGACAGAGACCTTTCTCACCGTAGCAGAACTGAGAGACCTTTTGGCTACCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sec3-PIP2_bind Sec3_C

Protein Analysis

886

Amino Acids

99.73

Weight (kDa)

5.51

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec3-PIP2_bind PF15277 50 - 144 1.1e-14 Exocyst complex component SEC3 N-terminal PIP2 binding PH
Sec3_CC PF09763 226 - 347 1.1e-25 Exocyst complex component Sec3, coiled-coil
Vps52_CC PF04129 258 - 395 2.8e-08 Vps52/Sac2 family, coiled-coil
Sec3_C-term PF20654 580 - 868 3e-27 Exocyst complex component Sec3, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1590
Acc36I ACCTGC 1 cut(s) 553
AccB1I GGYRCC 1 cut(s) 62
AccII CGCG 3 cut(s) 93, 141, 966
AclWI GGATC 5 cut(s) 82, 106, 1817, 2386, 2430
AcoI YGGCCR 4 cut(s) 96, 136, 147, 2247
AcsI RAATTY 4 cut(s) 1409, 2111, 2252, 2552
AcuI CTGAAG 3 cut(s) 1545, 1742, 2493
AfaI GTAC 7 cut(s) 316, 651, 1286, 1435, 1636, 2066, 2483
AfeI AGCGCT 1 cut(s) 1862
AfiI CCNNNNNNNGG 5 cut(s) 145, 156, 529, 613, 1631
AjnI CCWGG 6 cut(s) 24, 324, 1627, 1779, 2070, 2262
AloI GAACNNNNNNTCC 2 cut(s) 2322, 2354
Alw21I GWGCWC 1 cut(s) 928
Alw26I GTCTC 4 cut(s) 607, 1910, 2603, 2631
AlwI GGATC 5 cut(s) 82, 106, 1817, 2386, 2430
Aor51HI AGCGCT 1 cut(s) 1862
ApeKI GCWGC 9 cut(s) 548, 725, 791, 1502, 1739, 1833, 1889, 2198, 2521
ApoI RAATTY 4 cut(s) 1409, 2111, 2252, 2552
Asp700I GAANNNNTTC 1 cut(s) 1755
AspLEI GCGC 7 cut(s) 17, 966, 1081, 1352, 1591, 1863, 1995
AspS9I GGNCC 6 cut(s) 39, 187, 372, 542, 1090, 1249
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 4 cut(s) 683, 1928, 2252, 2611
AvaII GGWCC 3 cut(s) 372, 542, 1090
BaeGI GKGCMC 1 cut(s) 2241
BalI TGGCCA 3 cut(s) 98, 149, 2249
BanI GGYRCC 1 cut(s) 62
BauI CACGAG 1 cut(s) 1404
BbsI GAAGAC 2 cut(s) 870, 1791
Bbv12I GWGCWC 1 cut(s) 928
BbvCI CCTCAGC 1 cut(s) 612
BbvI GCAGC 9 cut(s) 535, 737, 803, 1489, 1751, 1820, 1901, 2185, 2508
BccI CCATC 4 cut(s) 62, 475, 1766, 2477
BceAI ACGGC 3 cut(s) 90, 1781, 2348
BcgI CGANNNNNNTGC 2 cut(s) 1931, 1965
BciT130I CCWGG 6 cut(s) 26, 326, 1629, 1781, 2072, 2264
BclI TGATCA 3 cut(s) 1267, 2095, 2310
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 4 cut(s) 607, 1910, 2603, 2631
BfaI CTAG 8 cut(s) 156, 306, 519, 956, 1200, 1431, 2082, 2225
BfmI CTRYAG 2 cut(s) 555, 1503
BfoI RGCGCY 3 cut(s) 1082, 1353, 1864
BfuAI ACCTGC 1 cut(s) 553
BglI GCCNNNNNGGC 1 cut(s) 47
Bme1390I CCNGG 7 cut(s) 26, 116, 326, 1629, 1781, 2072, 2264
Bme18I GGWCC 3 cut(s) 372, 542, 1090
BmgT120I GGNCC 6 cut(s) 39, 187, 372, 542, 1090, 1249
BmiI GGNNCC 5 cut(s) 64, 398, 543, 1092, 1250
BmrFI CCNGG 7 cut(s) 26, 116, 326, 1629, 1781, 2072, 2264
BmrI ACTGGG 1 cut(s) 1488
BmsI GCATC 6 cut(s) 280, 424, 560, 1451, 1530, 1876
BmuI ACTGGG 1 cut(s) 1488
BpiI GAAGAC 2 cut(s) 870, 1791
BpmI CTGGAG 1 cut(s) 1943
Bpu10I CCTNAGC 2 cut(s) 495, 612
BpuEI CTTGAG 3 cut(s) 263, 806, 2579
BpuMI CCSGG 1 cut(s) 116
Bsa29I ATCGAT 1 cut(s) 875
BsaAI YACGTR 1 cut(s) 1019
BsaI GGTCTC 2 cut(s) 2603, 2631
BsaJI CCNNGG 9 cut(s) 115, 150, 183, 324, 325, 523, 1628, 2071, 2244
BsaWI WCCGGW 1 cut(s) 230
BsaXI ACNNNNNCTCC 2 cut(s) 337, 367
Bsc4I CCNNNNNNNGG 5 cut(s) 145, 156, 529, 613, 1631
Bse1I ACTGG 2 cut(s) 1483, 2341
Bse3DI GCAATG 2 cut(s) 2002, 2484
BseBI CCWGG 6 cut(s) 26, 326, 1629, 1781, 2072, 2264
BseCI ATCGAT 1 cut(s) 875
BseDI CCNNGG 9 cut(s) 115, 150, 183, 324, 325, 523, 1628, 2071, 2244
BseGI GGATG 2 cut(s) 2025, 2067
BseLI CCNNNNNNNGG 5 cut(s) 145, 156, 529, 613, 1631
BseMI GCAATG 2 cut(s) 2002, 2484
BseMII CTCAG 6 cut(s) 603, 862, 966, 1167, 1256, 2624
BseNI ACTGG 2 cut(s) 1483, 2341
BseRI GAGGAG 3 cut(s) 249, 351, 354
BseSI GKGCMC 1 cut(s) 2241
BseX3I CGGCCG 1 cut(s) 136
BseXI GCAGC 9 cut(s) 535, 737, 803, 1489, 1751, 1820, 1901, 2185, 2508
Bsh1236I CGCG 3 cut(s) 93, 141, 966
Bsh1285I CGRYCG 1 cut(s) 139
BshNI GGYRCC 1 cut(s) 62
BshVI ATCGAT 1 cut(s) 875
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 928
BsiSI CCGG 2 cut(s) 115, 231
BslFI GGGAC 2 cut(s) 555, 2088
BslI CCNNNNNNNGG 5 cut(s) 145, 156, 529, 613, 1631
BsmAI GTCTC 4 cut(s) 607, 1910, 2603, 2631
BsmBI CGTCTC 1 cut(s) 1910
BsmFI GGGAC 2 cut(s) 555, 2088
BsmI GAATGC 1 cut(s) 1504
Bso31I GGTCTC 2 cut(s) 2603, 2631
Bsp1286I GDGCHC 2 cut(s) 928, 2241
Bsp1407I TGTACA 1 cut(s) 2481
BspCNI CTCAG 6 cut(s) 604, 861, 967, 1168, 1255, 2625
BspDI ATCGAT 1 cut(s) 875
BspFNI CGCG 3 cut(s) 93, 141, 966
BspLI GGNNCC 5 cut(s) 64, 398, 543, 1092, 1250
BspMAI CTGCAG 1 cut(s) 1507
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 5 cut(s) 82, 106, 1817, 2386, 2430
BspT107I GGYRCC 1 cut(s) 62
BspTNI GGTCTC 2 cut(s) 2603, 2631
BsrDI GCAATG 2 cut(s) 2002, 2484
BsrGI TGTACA 1 cut(s) 2481
BsrI ACTGG 2 cut(s) 1483, 2341
BssECI CCNNGG 9 cut(s) 115, 150, 183, 324, 325, 523, 1628, 2071, 2244
BssSI CACGAG 1 cut(s) 1404
BssT1I CCWWGG 4 cut(s) 150, 183, 523, 2244
Bst2BI CACGAG 1 cut(s) 1404
Bst2UI CCWGG 6 cut(s) 26, 326, 1629, 1781, 2072, 2264
Bst4CI ACNGT 6 cut(s) 559, 586, 1284, 1459, 1519, 2623
Bst6I CTCTTC 4 cut(s) 612, 695, 1761, 2382
BstAUI TGTACA 1 cut(s) 2481
BstBAI YACGTR 1 cut(s) 1019
BstC8I GCNNGC 8 cut(s) 39, 43, 249, 738, 1306, 1737, 1969, 2290
BstDEI CTNAG 8 cut(s) 341, 495, 612, 848, 975, 1176, 1242, 2633
BstF5I GGATG 2 cut(s) 2025, 2067
BstFNI CGCG 3 cut(s) 93, 141, 966
BstH2I RGCGCY 3 cut(s) 1082, 1353, 1864
BstHHI GCGC 7 cut(s) 17, 966, 1081, 1352, 1591, 1863, 1995
BstMAI GTCTC 4 cut(s) 607, 1910, 2603, 2631
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 9 cut(s) 47, 491, 722, 1502, 1889, 1909, 2045, 2273, 2304
BstNI CCWGG 6 cut(s) 26, 326, 1629, 1781, 2072, 2264
BstNSI RCATGY 3 cut(s) 133, 740, 990
BstSCI CCNGG 7 cut(s) 24, 114, 324, 1627, 1779, 2070, 2262
BstSFI CTRYAG 2 cut(s) 555, 1503
BstSLI GKGCMC 1 cut(s) 2241
BstUI CGCG 3 cut(s) 93, 141, 966
BstV1I GCAGC 9 cut(s) 535, 737, 803, 1489, 1751, 1820, 1901, 2185, 2508
BstV2I GAAGAC 2 cut(s) 870, 1791
BstX2I RGATCY 2 cut(s) 2391, 2422
BstXI CCANNNNNNTGG 1 cut(s) 1461
BstYI RGATCY 2 cut(s) 2391, 2422
BstZI CGGCCG 1 cut(s) 136
Bsu15I ATCGAT 1 cut(s) 875
BsuTUI ATCGAT 1 cut(s) 875
BtsCI GGATG 2 cut(s) 2025, 2067
BtsI GCAGTG 1 cut(s) 2472
BtsIMutI CAGTG 1 cut(s) 2472
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 8 cut(s) 39, 43, 249, 738, 1306, 1737, 1969, 2290
CfoI GCGC 7 cut(s) 17, 966, 1081, 1352, 1591, 1863, 1995
Cfr13I GGNCC 6 cut(s) 39, 187, 372, 542, 1090, 1249
ClaI ATCGAT 1 cut(s) 875
CseI GACGC 2 cut(s) 64, 1892
Csp6I GTAC 7 cut(s) 315, 650, 1285, 1434, 1635, 2065, 2482
CviQI GTAC 7 cut(s) 315, 650, 1285, 1434, 1635, 2065, 2482
DdeI CTNAG 8 cut(s) 341, 495, 612, 848, 975, 1176, 1242, 2633
DraI TTTAAA 1 cut(s) 1323
EaeI YGGCCR 4 cut(s) 96, 136, 147, 2247
EagI CGGCCG 1 cut(s) 136
Eam1104I CTCTTC 4 cut(s) 612, 695, 1761, 2382
EarI CTCTTC 4 cut(s) 612, 695, 1761, 2382
EciI GGCGGA 1 cut(s) 2435
EclXI CGGCCG 1 cut(s) 136
Eco130I CCWWGG 4 cut(s) 150, 183, 523, 2244
Eco147I AGGCCT 2 cut(s) 783, 1264
Eco31I GGTCTC 2 cut(s) 2603, 2631
Eco47I GGWCC 3 cut(s) 372, 542, 1090
Eco47III AGCGCT 1 cut(s) 1862
Eco52I CGGCCG 1 cut(s) 136
Eco57I CTGAAG 3 cut(s) 1545, 1742, 2493
EcoO109I RGGNCCY 1 cut(s) 542
EcoRII CCWGG 6 cut(s) 24, 324, 1627, 1779, 2070, 2262
EcoT14I CCWWGG 4 cut(s) 150, 183, 523, 2244
EcoT22I ATGCAT 1 cut(s) 1848
ErhI CCWWGG 4 cut(s) 150, 183, 523, 2244
Esp3I CGTCTC 1 cut(s) 1910
FalI AAGNNNNNCTT 6 cut(s) 192, 224, 1220, 1252, 2260, 2292
FaqI GGGAC 2 cut(s) 555, 2088
FauI CCCGC 3 cut(s) 30, 893, 1239
FauNDI CATATG 2 cut(s) 425, 1300
FbaI TGATCA 3 cut(s) 1267, 2095, 2310
FokI GGATG 2 cut(s) 2012, 2054
FspBI CTAG 8 cut(s) 156, 306, 519, 956, 1200, 1431, 2082, 2225
FspI TGCGCA 1 cut(s) 1590
GlaI GCGC 7 cut(s) 16, 965, 1080, 1351, 1590, 1862, 1994
GsuI CTGGAG 1 cut(s) 1943
HaeII RGCGCY 3 cut(s) 1082, 1353, 1864
HapII CCGG 2 cut(s) 115, 231
HgaI GACGC 2 cut(s) 64, 1892
HhaI GCGC 7 cut(s) 17, 966, 1081, 1352, 1591, 1863, 1995
Hin6I GCGC 7 cut(s) 15, 964, 1079, 1350, 1589, 1861, 1993
HinP1I GCGC 7 cut(s) 15, 964, 1079, 1350, 1589, 1861, 1993
HindIII AAGCTT 7 cut(s) 947, 1170, 1527, 1965, 2274, 2286, 2446
HinfI GANTC 9 cut(s) 213, 237, 346, 872, 1327, 1683, 1751, 1776, 1925
HpaII CCGG 2 cut(s) 115, 231
HphI GGTGA 4 cut(s) 683, 1928, 2252, 2611
Hpy166II GTNNAC 3 cut(s) 315, 755, 2373
Hpy8I GTNNAC 3 cut(s) 315, 755, 2373
Hpy99I CGWCG 4 cut(s) 80, 770, 1398, 2591
HpyAV CCTTC 6 cut(s) 53, 981, 1319, 2425, 2535, 2542
HpyCH4III ACNGT 6 cut(s) 559, 586, 1284, 1459, 1519, 2623
HpyCH4IV ACGT 2 cut(s) 644, 1018
HpyF10VI GCNNNNNNNGC 9 cut(s) 47, 491, 722, 1502, 1889, 1909, 2045, 2273, 2304
HpyF3I CTNAG 8 cut(s) 341, 495, 612, 848, 975, 1176, 1242, 2633
HpySE526I ACGT 2 cut(s) 644, 1018
HspAI GCGC 7 cut(s) 15, 964, 1079, 1350, 1589, 1861, 1993
Ksp22I TGATCA 3 cut(s) 1267, 2095, 2310
LmnI GCTCC 5 cut(s) 28, 364, 532, 1379, 2059
Lsp1109I GCAGC 9 cut(s) 535, 737, 803, 1489, 1751, 1820, 1901, 2185, 2508
LweI GCATC 6 cut(s) 280, 424, 560, 1451, 1530, 1876
MaeI CTAG 8 cut(s) 156, 306, 519, 956, 1200, 1431, 2082, 2225
MaeII ACGT 2 cut(s) 644, 1018
MaeIII GTNAC 6 cut(s) 514, 559, 569, 580, 1223, 2131
MfeI CAATTG 1 cut(s) 2376
MflI RGATCY 2 cut(s) 2391, 2422
MhlI GDGCHC 2 cut(s) 928, 2241
MlsI TGGCCA 3 cut(s) 98, 149, 2249
MluNI TGGCCA 3 cut(s) 98, 149, 2249
MlyI GAGTC 5 cut(s) 222, 246, 355, 1336, 1934
MmeI TCCRAC 2 cut(s) 879, 1478
Mox20I TGGCCA 3 cut(s) 98, 149, 2249
Mph1103I ATGCAT 1 cut(s) 1848
MroXI GAANNNNTTC 1 cut(s) 1755
MscI TGGCCA 3 cut(s) 98, 149, 2249
MseI TTAA 7 cut(s) 218, 293, 1119, 1322, 1620, 1745, 2438
MslI CAYNNNNRTG 2 cut(s) 1841, 2477
Msp20I TGGCCA 3 cut(s) 98, 149, 2249
MspA1I CMGCKG 2 cut(s) 512, 1246
MspI CCGG 2 cut(s) 115, 231
MspR9I CCNGG 7 cut(s) 26, 116, 326, 1629, 1781, 2072, 2264
MunI CAATTG 1 cut(s) 2376
Mva1269I GAATGC 1 cut(s) 1504
MvaI CCWGG 6 cut(s) 26, 326, 1629, 1781, 2072, 2264
MvnI CGCG 3 cut(s) 93, 141, 966
MwoI GCNNNNNNNGC 9 cut(s) 47, 491, 722, 1502, 1889, 1909, 2045, 2273, 2304
NciI CCSGG 1 cut(s) 116
NdeI CATATG 2 cut(s) 425, 1300
NlaIV GGNNCC 5 cut(s) 64, 398, 543, 1092, 1250
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 4 cut(s) 514, 569, 1223, 2131
NsbI TGCGCA 1 cut(s) 1590
NsiI ATGCAT 1 cut(s) 1848
NspI RCATGY 3 cut(s) 133, 740, 990
PaeI GCATGC 1 cut(s) 740
PasI CCCWGGG 1 cut(s) 325
PceI AGGCCT 2 cut(s) 783, 1264
PcsI WCGNNNNNNNCGW 1 cut(s) 1402
PctI GAATGC 1 cut(s) 1504
PdmI GAANNNNTTC 1 cut(s) 1755
PfeI GAWTC 4 cut(s) 872, 1683, 1751, 1776
PfoI TCCNGGA 1 cut(s) 1779
PleI GAGTC 5 cut(s) 221, 245, 354, 1335, 1933
PpsI GAGTC 5 cut(s) 221, 245, 354, 1335, 1933
Ppu21I YACGTR 1 cut(s) 1019
PpuMI RGGWCCY 1 cut(s) 542
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 6 cut(s) 24, 324, 1627, 1779, 2070, 2262
PspGI CCWGG 6 cut(s) 24, 324, 1627, 1779, 2070, 2262
PspN4I GGNNCC 5 cut(s) 64, 398, 543, 1092, 1250
PspPI GGNCC 6 cut(s) 39, 187, 372, 542, 1090, 1249
PspPPI RGGWCCY 1 cut(s) 542
PstI CTGCAG 1 cut(s) 1507
PsuI RGATCY 2 cut(s) 2391, 2422
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 7 cut(s) 316, 651, 1286, 1435, 1636, 2066, 2483
RsaNI GTAC 7 cut(s) 315, 650, 1285, 1434, 1635, 2065, 2482
RseI CAYNNNNRTG 2 cut(s) 1841, 2477
SaqAI TTAA 7 cut(s) 218, 293, 1119, 1322, 1620, 1745, 2438
Sau96I GGNCC 6 cut(s) 39, 187, 372, 542, 1090, 1249
SchI GAGTC 5 cut(s) 222, 246, 355, 1336, 1934
ScrFI CCNGG 7 cut(s) 26, 116, 326, 1629, 1781, 2072, 2264
SduI GDGCHC 2 cut(s) 928, 2241
SfaNI GCATC 6 cut(s) 280, 424, 560, 1451, 1530, 1876
SfcI CTRYAG 2 cut(s) 555, 1503
SfiI GGCCNNNNNGGCC 1 cut(s) 47
SinI GGWCC 3 cut(s) 372, 542, 1090
SmiMI CAYNNNNRTG 2 cut(s) 1841, 2477
SmlI CTYRAG 3 cut(s) 242, 785, 2558
SmoI CTYRAG 3 cut(s) 242, 785, 2558
SphI GCATGC 1 cut(s) 740
SseBI AGGCCT 2 cut(s) 783, 1264
SspI AATATT 3 cut(s) 224, 385, 2168
SspMI CTAG 8 cut(s) 156, 306, 519, 956, 1200, 1431, 2082, 2225
StuI AGGCCT 2 cut(s) 783, 1264
StyD4I CCNGG 7 cut(s) 24, 114, 324, 1627, 1779, 2070, 2262
StyI CCWWGG 4 cut(s) 150, 183, 523, 2244
TaaI ACNGT 6 cut(s) 559, 586, 1284, 1459, 1519, 2623
TaiI ACGT 2 cut(s) 647, 1021
TatI WGTACW 5 cut(s) 314, 649, 1433, 2064, 2481
TauI GCSGC 3 cut(s) 53, 141, 986
TfiI GAWTC 4 cut(s) 872, 1683, 1751, 1776
Tru1I TTAA 7 cut(s) 218, 293, 1119, 1322, 1620, 1745, 2438
Tru9I TTAA 7 cut(s) 218, 293, 1119, 1322, 1620, 1745, 2438
TscAI CASTG 1 cut(s) 2479
TseFI GTSAC 4 cut(s) 514, 569, 1223, 2131
TseI GCWGC 9 cut(s) 548, 725, 791, 1502, 1739, 1833, 1889, 2198, 2521
Tsp45I GTSAC 4 cut(s) 514, 569, 1223, 2131
TspDTI ATGAA 5 cut(s) 993, 1026, 1764, 1977, 2583
TspGWI ACGGA 2 cut(s) 163, 578
TspRI CASTG 1 cut(s) 2479
VpaK11BI GGWCC 3 cut(s) 372, 542, 1090
XapI RAATTY 4 cut(s) 1409, 2111, 2252, 2552
XceI RCATGY 3 cut(s) 133, 740, 990
XmnI GAANNNNTTC 1 cut(s) 1755
XspI CTAG 8 cut(s) 156, 306, 519, 956, 1200, 1431, 2082, 2225
Zsp2I ATGCAT 1 cut(s) 1848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.