Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0014122

Physical Location & Seq

Genomic Coordinates Forward (+)

3530664 .. 3534639

Gene Structure 3976 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGCATAGAGCAAAAGCCATTGAAGCTCTGAGGAGGTGTGCGGCTCAGAGAGGAAGAAGCAGCTCTCTCTCTCATCGCAGAGCCTTTTCTGCCCAACCAAGCTATGCCCAATACGACGACGTCCAGGACCTGGTTACGGTGGAGGGAAGAGCCAAATCGAGAGCGGCAATTCTCAACAGGCCATCTGCACTCAATGCTCTCAACAGTTCCATGGCAGCTCGACTGAAGAGACTGTATGAATCATGGGAGGATAACCCGGACATTGGATTTGTCTTGATGAAGGGTAGTGGGAGGGCTTTCTGCGCCGGAGCAGATGCTGTTAGCCTTTATCAACTAGTGAATGAAGGGAATCTTGATGAATGTAAAAAGTTTTTCGAGACATTGTATAAATTTGTGTATATTCAAGGAACGTACTTGAAGCCACACGTAGCCATCATGGATGGTATCACCATGGGAGCTGGGGCTGGAATTTCAATCCCAGGAATGTTTCGCGTGGTGACTGATAAGACTATATTTTCCAATCCAGAGGCCCAGATAGGTTTCCATCCTGATGCAGGGGCTTCCTTTTATCTGTCTCGTCTACCTGGCTACCTAGGAGAATACTTAGCTCTTACAGGAGACAAGCTTAATGGTGTGGAGATGGTTGCCTGTCGCCTTGCTACGCACTATGCGTTAAATGCGAGACTTGCTATGGTTGAAGAACGCCTTGGAAATTTAGTTACAGATGAACCTTCTGTAATAGAAACAGCTCTTGCACAATATGGTGACCTTGTTTATCTAGACAGGAGGAGTATACTTTCTAAGCTCGACGTTATTGATAGATGTTTCAGCCATGACACAATTGAGGAAATTACTGATGCTTTGGAGAAGGAAGCTGCTGACTCTTATGATGAATGGTGCAAAACAGTCCTCAAGAAAATAAAAGAAGCCTCCCCCTTGAGTTTGATAGTTTCATTACGATCGATACGCGAGGGTAGATTTCAATCTCTAGATCAGTGTCTGGCTCGTGAATATCGTATATCTCTTGCTGCAATTTCTAACCGGGTCTCGAGTGACTTCTCTGAGGGCGTTCGAGCAAGATTGGTAGACAAGGACTTTGCGCCCAAGTGGAACCCCCCTAGTTTCAAAGATGTTTCCAAAGACATGGTTGATTGCTATTTCTCACCGCTTCCTGACGTTGAGCCGGAGCTGAAGCTGCCTACAGCATTGCGAGAGCCATTTATGCAAGAGACGGATTCACCCAAAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

417

Amino Acids

46.36

Weight (kDa)

6.16

Isoelectric Point (pI)

39.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_2	PF16113	54 - 387	9.5e-117	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	58 - 213	6.1e-20	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014122)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G31810
fragaria_vesca	FvH4_2g41110
malus_domestica	MD08G1057200.v1.1 MD15G1040200.v1.1
prunus_persica	Prupe.1G393600_v2.0.a1 Prupe.1G393600_v2.0.a1 Prupe.1G393600_v2.0.a1
pyrus_communis	pycom08g04670
rosa_chinensis	RchiOBHm_Chr6g0299431
rosa_laevigata	RLG00000011432
rosa_multiflora	Rmu_sc0037301.1_g000004
rosa_roxburghii	Rroxscaffold_7G00168690
rosa_rugosa	Rorug06G0288100
rosa_samantha	Rh6AG400000 Rh6BG408600 Rh6CG414100
rosa_wichuraiana	Rw6G035010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	123
AccB7I	CCANNNNNTGG	1 cut(s)	130
AccBSI	CCGCTC	1 cut(s)	164
AccI	GTMKAC	3 cut(s)	580, 793, 1086
AccII	CGCG	2 cut(s)	492, 969
AciI	CCGC	3 cut(s)	41, 164, 1166
AcsI	RAATTY	3 cut(s)	389, 468, 712
AcuI	CTGAAG	2 cut(s)	245, 1211
AcyI	GRCGYC	1 cut(s)	120
AfaI	GTAC	1 cut(s)	413
AfiI	CCNNNNNNNGG	4 cut(s)	130, 136, 263, 554
AflIII	ACRYGT	1 cut(s)	424
AgsI	TTSAA	7 cut(s)	23, 404, 418, 474, 698, 983, 1126
AhlI	ACTAGT	1 cut(s)	334
AjnI	CCWGG	4 cut(s)	123, 129, 478, 583
AjuI	GAANNNNNNNTTGG	2 cut(s)	690, 722
Alw26I	GTCTC	7 cut(s)	223, 371, 579, 612, 676, 1051, 1223
AlwNI	CAGNNNCTG	3 cut(s)	130, 317, 1000
Ama87I	CYCGRG	1 cut(s)	1048
AoxI	GGCC	2 cut(s)	179, 528
ApeKI	GCWGC	5 cut(s)	60, 215, 875, 1028, 1195
ApoI	RAATTY	3 cut(s)	389, 468, 712
ArsI	GACNNNNNNTTYG	4 cut(s)	251, 283, 1079, 1111
AspA2I	CCTAGG	1 cut(s)	592
AspLEI	GCGC	2 cut(s)	305, 1102
AspS9I	GGNCC	2 cut(s)	127, 529
AsuC2I	CCSGG	2 cut(s)	257, 1043
AsuHPI	GGTGA	5 cut(s)	439, 508, 776, 1155, 1230
AvaI	CYCGRG	1 cut(s)	1048
AvaII	GGWCC	1 cut(s)	127
AvrII	CCTAGG	1 cut(s)	592
BauI	CACGAG	1 cut(s)	1005
BbvI	GCAGC	5 cut(s)	72, 227, 862, 1015, 1182
BccI	CCATC	5 cut(s)	190, 434, 440, 552, 634
BciT130I	CCWGG	4 cut(s)	125, 131, 480, 585
BcnI	CCSGG	2 cut(s)	257, 1043
BcoDI	GTCTC	7 cut(s)	223, 371, 579, 612, 676, 1051, 1223
BcuI	ACTAGT	1 cut(s)	334
BfaI	CTAG	5 cut(s)	335, 593, 779, 989, 1119
BfmI	CTRYAG	1 cut(s)	1200
BisI	GCNGC	7 cut(s)	42, 61, 165, 216, 876, 1029, 1196
BlnI	CCTAGG	1 cut(s)	592
BlsI	GCNGC	7 cut(s)	43, 62, 166, 217, 877, 1030, 1197
Bme1390I	CCNGG	6 cut(s)	125, 131, 257, 480, 585, 1043
Bme18I	GGWCC	1 cut(s)	127
BmeT110I	CYCGRG	1 cut(s)	1048
BmgT120I	GGNCC	2 cut(s)	127, 529
BmiI	GGNNCC	1 cut(s)	1112
BmrFI	CCNGG	6 cut(s)	125, 131, 257, 480, 585, 1043
BmsI	GCATC	3 cut(s)	304, 541, 847
BpuEI	CTTGAG	2 cut(s)	896, 958
BpuMI	CCSGG	2 cut(s)	257, 1043
Bsa29I	ATCGAT	1 cut(s)	962
BsaAI	YACGTR	1 cut(s)	427
BsaBI	GATNNNNATC	1 cut(s)	982
BsaHI	GRCGYC	1 cut(s)	120
BsaI	GGTCTC	1 cut(s)	1051
BsaJI	CCNNGG	5 cut(s)	210, 450, 478, 592, 706
Bsc4I	CCNNNNNNNGG	4 cut(s)	130, 136, 263, 554
Bse3DI	GCAATG	1 cut(s)	1205
Bse8I	GATNNNNATC	1 cut(s)	982
BseBI	CCWGG	4 cut(s)	125, 131, 480, 585
BseCI	ATCGAT	1 cut(s)	962
BseDI	CCNNGG	5 cut(s)	210, 450, 478, 592, 706
BseGI	GGATG	2 cut(s)	445, 544
BseJI	GATNNNNATC	1 cut(s)	982
BseLI	CCNNNNNNNGG	4 cut(s)	130, 136, 263, 554
BseMI	GCAATG	1 cut(s)	1205
BseMII	CTCAG	3 cut(s)	20, 59, 1053
BseRI	GAGGAG	2 cut(s)	46, 802
BseXI	GCAGC	5 cut(s)	72, 227, 862, 1015, 1182
BseYI	CCCAGC	1 cut(s)	458
BsgI	GTGCAG	1 cut(s)	171
Bsh1236I	CGCG	2 cut(s)	492, 969
Bsh1285I	CGRYCG	1 cut(s)	962
BshFI	GGCC	2 cut(s)	181, 530
BshVI	ATCGAT	1 cut(s)	962
BsiEI	CGRYCG	1 cut(s)	962
BsiHKCI	CYCGRG	1 cut(s)	1048
BsiSI	CCGG	4 cut(s)	257, 306, 1042, 1184
BslI	CCNNNNNNNGG	4 cut(s)	130, 136, 263, 554
BsmAI	GTCTC	7 cut(s)	223, 371, 579, 612, 676, 1051, 1223
BsmBI	CGTCTC	1 cut(s)	1223
BsnI	GGCC	2 cut(s)	181, 530
Bso31I	GGTCTC	1 cut(s)	1051
BsoBI	CYCGRG	1 cut(s)	1048
Bsp143I	GATC	2 cut(s)	959, 991
Bsp19I	CCATGG	2 cut(s)	210, 450
BspACI	CCGC	3 cut(s)	41, 164, 1166
BspANI	GGCC	2 cut(s)	181, 530
BspCNI	CTCAG	3 cut(s)	21, 58, 1054
BspDI	ATCGAT	1 cut(s)	962
BspFNI	CGCG	2 cut(s)	492, 969
BspLI	GGNNCC	1 cut(s)	1112
BspQI	GCTCTTC	1 cut(s)	142
BspTNI	GGTCTC	1 cut(s)	1051
BsrBI	CCGCTC	1 cut(s)	164
BsrDI	GCAATG	1 cut(s)	1205
BssECI	CCNNGG	5 cut(s)	210, 450, 478, 592, 706
BssMI	GATC	2 cut(s)	959, 991
BssNAI	GTATAC	1 cut(s)	794
BssNI	GRCGYC	1 cut(s)	120
BssSI	CACGAG	1 cut(s)	1005
BssT1I	CCWWGG	4 cut(s)	210, 450, 592, 706
Bst1107I	GTATAC	1 cut(s)	794
Bst2BI	CACGAG	1 cut(s)	1005
Bst2UI	CCWGG	4 cut(s)	125, 131, 480, 585
Bst4CI	ACNGT	4 cut(s)	139, 206, 234, 907
Bst6I	CTCTTC	2 cut(s)	142, 221
BstACI	GRCGYC	1 cut(s)	120
BstAPI	GCANNNNNTGC	1 cut(s)	194
BstBAI	YACGTR	1 cut(s)	427
BstDEI	CTNAG	5 cut(s)	29, 45, 604, 801, 1062
BstDSI	CCRYGG	2 cut(s)	210, 450
BstEII	GGTNACC	1 cut(s)	764
BstENI	CCTNNNNNAGG	1 cut(s)	552
BstF5I	GGATG	2 cut(s)	445, 544
BstFNI	CGCG	2 cut(s)	492, 969
BstHHI	GCGC	2 cut(s)	305, 1102
BstKTI	GATC	2 cut(s)	962, 994
BstMAI	GTCTC	7 cut(s)	223, 371, 579, 612, 676, 1051, 1223
BstMBI	GATC	2 cut(s)	959, 991
BstMCI	CGRYCG	1 cut(s)	962
BstMWI	GCNNNNNNNGC	8 cut(s)	23, 89, 194, 302, 677, 686, 1195, 1222
BstNI	CCWGG	4 cut(s)	125, 131, 480, 585
BstPI	GGTNACC	1 cut(s)	764
BstSCI	CCNGG	6 cut(s)	123, 129, 255, 478, 583, 1041
BstSFI	CTRYAG	1 cut(s)	1200
BstUI	CGCG	2 cut(s)	492, 969
BstV1I	GCAGC	5 cut(s)	72, 227, 862, 1015, 1182
BstXI	CCANNNNNNTGG	1 cut(s)	1144
BstZ17I	GTATAC	1 cut(s)	794
Bsu15I	ATCGAT	1 cut(s)	962
BsuRI	GGCC	2 cut(s)	181, 530
BsuTUI	ATCGAT	1 cut(s)	962
BtgI	CCRYGG	2 cut(s)	210, 450
BtgZI	GCGATG	1 cut(s)	59
BtsCI	GGATG	2 cut(s)	445, 544
BtsIMutI	CAGTG	1 cut(s)	1001
CaiI	CAGNNNCTG	3 cut(s)	130, 317, 1000
CfoI	GCGC	2 cut(s)	305, 1102
Cfr13I	GGNCC	2 cut(s)	127, 529
ClaI	ATCGAT	1 cut(s)	962
CsiI	ACCWGGT	1 cut(s)	129
Csp6I	GTAC	1 cut(s)	412
CviAII	CATG	6 cut(s)	211, 243, 436, 451, 833, 1144
CviQI	GTAC	1 cut(s)	412
DdeI	CTNAG	5 cut(s)	29, 45, 604, 801, 1062
DpnI	GATC	2 cut(s)	961, 993
DpnII	GATC	2 cut(s)	959, 991
Eam1104I	CTCTTC	2 cut(s)	142, 221
EarI	CTCTTC	2 cut(s)	142, 221
Eco130I	CCWWGG	4 cut(s)	210, 450, 592, 706
Eco31I	GGTCTC	1 cut(s)	1051
Eco47I	GGWCC	1 cut(s)	127
Eco57I	CTGAAG	2 cut(s)	245, 1211
Eco88I	CYCGRG	1 cut(s)	1048
Eco91I	GGTNACC	1 cut(s)	764
EcoNI	CCTNNNNNAGG	1 cut(s)	552
EcoO109I	RGGNCCY	1 cut(s)	127
EcoO65I	GGTNACC	1 cut(s)	764
EcoRII	CCWGG	4 cut(s)	123, 129, 478, 583
EcoT14I	CCWWGG	4 cut(s)	210, 450, 592, 706
EcoT22I	ATGCAT	1 cut(s)	6
ErhI	CCWWGG	4 cut(s)	210, 450, 592, 706
Esp3I	CGTCTC	1 cut(s)	1223
FaeI	CATG	6 cut(s)	214, 246, 439, 454, 836, 1147
FalI	AAGNNNNNCTT	4 cut(s)	336, 368, 690, 722
FatI	CATG	6 cut(s)	210, 242, 435, 450, 832, 1143
FblI	GTMKAC	3 cut(s)	580, 793, 1086
Fnu4HI	GCNGC	7 cut(s)	42, 61, 165, 216, 876, 1029, 1196
FokI	GGATG	2 cut(s)	452, 531
Fsp4HI	GCNGC	7 cut(s)	42, 61, 165, 216, 876, 1029, 1196
FspBI	CTAG	5 cut(s)	335, 593, 779, 989, 1119
GlaI	GCGC	2 cut(s)	304, 1101
GluI	GCNGC	7 cut(s)	42, 61, 165, 216, 876, 1029, 1196
GsaI	CCCAGC	1 cut(s)	462
HaeIII	GGCC	2 cut(s)	181, 530
HapII	CCGG	4 cut(s)	257, 306, 1042, 1184
HhaI	GCGC	2 cut(s)	305, 1102
Hin1I	GRCGYC	1 cut(s)	120
Hin1II	CATG	6 cut(s)	214, 246, 439, 454, 836, 1147
Hin6I	GCGC	2 cut(s)	303, 1100
HinP1I	GCGC	2 cut(s)	303, 1100
HindIII	AAGCTT	1 cut(s)	623
HinfI	GANTC	4 cut(s)	239, 349, 881, 1235
HpaII	CCGG	4 cut(s)	257, 306, 1042, 1184
HphI	GGTGA	5 cut(s)	439, 508, 776, 1155, 1230
Hpy166II	GTNNAC	3 cut(s)	581, 794, 1087
Hpy188I	TCNGA	3 cut(s)	30, 48, 1063
Hpy8I	GTNNAC	3 cut(s)	581, 794, 1087
Hpy99I	CGWCG	3 cut(s)	119, 122, 812
HpyAV	CCTTC	4 cut(s)	274, 338, 741, 862
HpyCH4III	ACNGT	4 cut(s)	139, 206, 234, 907
HpyCH4IV	ACGT	5 cut(s)	120, 410, 426, 810, 1176
HpyCH4V	TGCA	7 cut(s)	4, 188, 554, 755, 900, 1031, 1225
HpyF10VI	GCNNNNNNNGC	8 cut(s)	23, 89, 194, 302, 677, 686, 1195, 1222
HpyF3I	CTNAG	5 cut(s)	29, 45, 604, 801, 1062
HpySE526I	ACGT	5 cut(s)	120, 410, 426, 810, 1176
Hsp92I	GRCGYC	1 cut(s)	120
Hsp92II	CATG	6 cut(s)	214, 246, 439, 454, 836, 1147
HspAI	GCGC	2 cut(s)	303, 1100
Kzo9I	GATC	2 cut(s)	959, 991
LguI	GCTCTTC	1 cut(s)	142
LmnI	GCTCC	3 cut(s)	308, 455, 1186
Lsp1109I	GCAGC	5 cut(s)	72, 227, 862, 1015, 1182
LweI	GCATC	3 cut(s)	304, 541, 847
MabI	ACCWGGT	1 cut(s)	129
MaeI	CTAG	5 cut(s)	335, 593, 779, 989, 1119
MaeII	ACGT	5 cut(s)	120, 410, 426, 810, 1176
MaeIII	GTNAC	5 cut(s)	133, 496, 718, 764, 1052
MalI	GATC	2 cut(s)	961, 993
MbiI	CCGCTC	1 cut(s)	164
MboI	GATC	2 cut(s)	959, 991
MboII	GAAGA	4 cut(s)	66, 159, 238, 710
MfeI	CAATTG	1 cut(s)	840
MluCI	AATT	7 cut(s)	168, 389, 468, 712, 840, 849, 1032
MlyI	GAGTC	1 cut(s)	875
Mph1103I	ATGCAT	1 cut(s)	6
MseI	TTAA	2 cut(s)	627, 674
MslI	CAYNNNNRTG	1 cut(s)	549
MspI	CCGG	4 cut(s)	257, 306, 1042, 1184
MspR9I	CCNGG	6 cut(s)	125, 131, 257, 480, 585, 1043
MunI	CAATTG	1 cut(s)	840
MvaI	CCWGG	4 cut(s)	125, 131, 480, 585
MvnI	CGCG	2 cut(s)	492, 969
MwoI	GCNNNNNNNGC	8 cut(s)	23, 89, 194, 302, 677, 686, 1195, 1222
NciI	CCSGG	2 cut(s)	257, 1043
NcoI	CCATGG	2 cut(s)	210, 450
NdeII	GATC	2 cut(s)	959, 991
NlaIII	CATG	6 cut(s)	214, 246, 439, 454, 836, 1147
NlaIV	GGNNCC	1 cut(s)	1112
NmuCI	GTSAC	3 cut(s)	496, 764, 1052
NsiI	ATGCAT	1 cut(s)	6
PaeR7I	CTCGAG	1 cut(s)	1048
PciSI	GCTCTTC	1 cut(s)	142
PcsI	WCGNNNNNNNCGW	2 cut(s)	668, 1012
PfeI	GAWTC	3 cut(s)	239, 349, 1235
PflFI	GACNNNGTC	1 cut(s)	119
PflMI	CCANNNNNTGG	1 cut(s)	130
PfoI	TCCNGGA	1 cut(s)	123
PkrI	GCNGC	7 cut(s)	43, 62, 166, 217, 877, 1030, 1197
Ple19I	CGATCG	1 cut(s)	962
PleI	GAGTC	1 cut(s)	875
PpsI	GAGTC	1 cut(s)	875
Ppu21I	YACGTR	1 cut(s)	427
PpuMI	RGGWCCY	1 cut(s)	127
Psp5II	RGGWCCY	1 cut(s)	127
Psp6I	CCWGG	4 cut(s)	123, 129, 478, 583
PspEI	GGTNACC	1 cut(s)	764
PspFI	CCCAGC	1 cut(s)	458
PspGI	CCWGG	4 cut(s)	123, 129, 478, 583
PspN4I	GGNNCC	1 cut(s)	1112
PspPI	GGNCC	2 cut(s)	127, 529
PspPPI	RGGWCCY	1 cut(s)	127
PstNI	CAGNNNCTG	3 cut(s)	130, 317, 1000
PsyI	GACNNNGTC	1 cut(s)	119
PvuI	CGATCG	1 cut(s)	962
RsaI	GTAC	1 cut(s)	413
RsaNI	GTAC	1 cut(s)	412
RseI	CAYNNNNRTG	1 cut(s)	549
SapI	GCTCTTC	1 cut(s)	142
SaqAI	TTAA	2 cut(s)	627, 674
SatI	GCNGC	7 cut(s)	42, 61, 165, 216, 876, 1029, 1196
Sau3AI	GATC	2 cut(s)	959, 991
Sau96I	GGNCC	2 cut(s)	127, 529
SchI	GAGTC	1 cut(s)	875
ScrFI	CCNGG	6 cut(s)	125, 131, 257, 480, 585, 1043
SexAI	ACCWGGT	1 cut(s)	129
SfaNI	GCATC	3 cut(s)	304, 541, 847
SfcI	CTRYAG	1 cut(s)	1200
Sfr274I	CTCGAG	1 cut(s)	1048
SinI	GGWCC	1 cut(s)	127
SlaI	CTCGAG	1 cut(s)	1048
SmiMI	CAYNNNNRTG	1 cut(s)	549
SmlI	CTYRAG	3 cut(s)	911, 937, 1048
SmoI	CTYRAG	3 cut(s)	911, 937, 1048
SpeI	ACTAGT	1 cut(s)	334
Sse9I	AATT	7 cut(s)	168, 389, 468, 712, 840, 849, 1032
SsiI	CCGC	3 cut(s)	41, 164, 1166
SspMI	CTAG	5 cut(s)	335, 593, 779, 989, 1119
StyD4I	CCNGG	6 cut(s)	123, 129, 255, 478, 583, 1041
StyI	CCWWGG	4 cut(s)	210, 450, 592, 706
TaaI	ACNGT	4 cut(s)	139, 206, 234, 907
TaiI	ACGT	5 cut(s)	123, 413, 429, 813, 1179
TaqI	TCGA	7 cut(s)	158, 220, 375, 807, 962, 1049, 1072
TasI	AATT	7 cut(s)	168, 389, 468, 712, 840, 849, 1032
TauI	GCSGC	2 cut(s)	44, 167
TfiI	GAWTC	3 cut(s)	239, 349, 1235
Tru1I	TTAA	2 cut(s)	627, 674
Tru9I	TTAA	2 cut(s)	627, 674
TscAI	CASTG	1 cut(s)	1001
TseFI	GTSAC	3 cut(s)	496, 764, 1052
TseI	GCWGC	5 cut(s)	60, 215, 875, 1028, 1195
Tsp45I	GTSAC	3 cut(s)	496, 764, 1052
TspDTI	ATGAA	7 cut(s)	252, 293, 357, 372, 741, 906, 942
TspGWI	ACGGA	1 cut(s)	1247
TspRI	CASTG	1 cut(s)	1001
Tth111I	GACNNNGTC	1 cut(s)	119
Van91I	CCANNNNNTGG	1 cut(s)	130
VpaK11BI	GGWCC	1 cut(s)	127
XagI	CCTNNNNNAGG	1 cut(s)	552
XapI	RAATTY	3 cut(s)	389, 468, 712
XbaI	TCTAGA	2 cut(s)	778, 988
XhoI	CTCGAG	1 cut(s)	1048
XmaJI	CCTAGG	1 cut(s)	592
XmiI	GTMKAC	3 cut(s)	580, 793, 1086
XspI	CTAG	5 cut(s)	335, 593, 779, 989, 1119
ZraI	GACGTC	1 cut(s)	121
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.