pycom08g05300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0016765
Physical Location & Seq
Reverse (-)
4089816 .. 4090595
780 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05300.1

Sequence Viewer

Length: 780 bp
ATGGGAAGAAGAATTTCCACAGATGAGGCAAACCTGCTAATCCATCCGTTGCTATCTTTGTCTTTCTTTGCTGCTGCCATGGCCGCCACCTTTGCCATAATCACGGCTCTTTGTAGTTTTCGAAGAAAATCTTCTCCGCCCCCTTCTGAATCCCCTGCTTCTAAGGAAGCAGAGGAATCCGATGCTTGCAATGTGCAAACATCGGAAGCTGCATTGGCGGAGACGGGGAACAAAACACCTCAACAAGATTTGGAAAGAGATCAAGAAAATGAGGATCCAATGATGAGTAAAGAGCTGCCTTTGCCTCCAAGAATGAAGAACTCAGGACAACTTTATTCAGGACAGCTTTATTCCGGCAACCTCGACATGAAACCTAAATTGAAAAAGTCTGCTTCCGAGCGAAGGCTCTTCTCGAATTTGAGCATTAAATTGCCACGAAGCTTGTCGATGGCACGAAAAGATAAGGTTAAGGAAGAGTACAACAAGCGAAAGAACGGAAAGTTGAAGCCGGAAGACTCAATCTGGATGAAGACGATCATACTAGGTGAGAAATGTAAAGTACCTGATGAGGATGAAGCTGTGATATATGATGCTAAGGGGAGGAGGATTCCGGCTTATCGCCCAAAAAGTAGGCAATCCTCCTTCATTGATCCGAACGCATTACCGGACCAAAGAATGAAAAGAGTAGAAGTAGAAAACAAAATTAATCAAGAGGCCGAGCACAAACAAGAAAACAAACACCAAGAAAGTGATCAAGAACATAACGATGAGGATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

29.37

Weight (kDa)

7.67

Isoelectric Point (pI)

61.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016765)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g31620
malus_domestica MD15G1056900.v1.1
prunus_persica Prupe.1G409500_v2.0.a1
pyrus_communis pycom08g05300 pycom15g05330
rosa_chinensis RchiOBHm_Chr6g0311201
rosa_laevigata RLG00000010428
rosa_multiflora Rmu_co8149324.1_g000001
rosa_roxburghii Rroxscaffold_7G00157910
rosa_rugosa Rorug06G0386300
rosa_samantha Rh6AG500500 Rh6BG511100 Rh6CG516900 Rh6DG503400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 42
AciI CCGC 3 cut(s) 84, 137, 218
AclWI GGATC 3 cut(s) 269, 282, 644
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 2 cut(s) 12, 415
AfaI GTAC 2 cut(s) 479, 561
AfiI CCNNNNNNNGG 1 cut(s) 402
AgsI TTSAA 2 cut(s) 382, 505
AluBI AGCT 5 cut(s) 209, 295, 346, 441, 578
AluI AGCT 5 cut(s) 209, 295, 346, 441, 578
Alw21I GWGCWC 1 cut(s) 723
Alw26I GTCTC 1 cut(s) 215
AlwI GGATC 3 cut(s) 269, 282, 644
AoxI GGCC 2 cut(s) 81, 714
ApeKI GCWGC 4 cut(s) 71, 74, 209, 295
ApoI RAATTY 2 cut(s) 12, 415
AseI ATTAAT 1 cut(s) 705
Asp700I GAANNNNTTC 2 cut(s) 13, 130
AspS9I GGNCC 1 cut(s) 667
AsuHPI GGTGA 1 cut(s) 557
AsuII TTCGAA 1 cut(s) 121
AvaII GGWCC 1 cut(s) 667
BamHI GGATCC 1 cut(s) 274
BbsI GAAGAC 2 cut(s) 519, 536
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 4 cut(s) 58, 61, 196, 282
BccI CCATC 2 cut(s) 51, 442
BceAI ACGGC 1 cut(s) 120
BclI TGATCA 1 cut(s) 751
BcoDI GTCTC 1 cut(s) 215
BfaI CTAG 1 cut(s) 542
BfuAI ACCTGC 1 cut(s) 42
BisI GCNGC 5 cut(s) 72, 75, 84, 210, 296
BlsI GCNGC 5 cut(s) 73, 76, 85, 211, 297
Bme18I GGWCC 1 cut(s) 667
BmgT120I GGNCC 1 cut(s) 667
BmiI GGNNCC 1 cut(s) 276
BmsI GCATC 2 cut(s) 172, 580
BpiI GAAGAC 2 cut(s) 519, 536
Bpu10I CCTNAGC 1 cut(s) 594
Bpu14I TTCGAA 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 78
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse3DI GCAATG 1 cut(s) 196
BseDI CCNNGG 1 cut(s) 78
BseGI GGATG 3 cut(s) 43, 531, 577
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMI GCAATG 1 cut(s) 196
BseMII CTCAG 1 cut(s) 336
BseRI GAGGAG 1 cut(s) 616
BseXI GCAGC 4 cut(s) 58, 61, 196, 282
BshFI GGCC 2 cut(s) 83, 716
BsiHKAI GWGCWC 1 cut(s) 723
BsiSI CCGG 4 cut(s) 354, 509, 611, 665
BslI CCNNNNNNNGG 1 cut(s) 402
BsmAI GTCTC 1 cut(s) 215
BsmBI CGTCTC 1 cut(s) 215
BsnI GGCC 2 cut(s) 83, 716
Bsp119I TTCGAA 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 723
Bsp143I GATC 5 cut(s) 259, 274, 534, 649, 751
Bsp19I CCATGG 1 cut(s) 78
BspACI CCGC 3 cut(s) 84, 137, 218
BspANI GGCC 2 cut(s) 83, 716
BspCNI CTCAG 1 cut(s) 335
BspLI GGNNCC 1 cut(s) 276
BspMI ACCTGC 1 cut(s) 42
BspPI GGATC 3 cut(s) 269, 282, 644
BspQI GCTCTTC 1 cut(s) 413
BspT104I TTCGAA 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 5 cut(s) 259, 274, 534, 649, 751
BssT1I CCWWGG 1 cut(s) 78
Bst6I CTCTTC 2 cut(s) 413, 468
BstBI TTCGAA 1 cut(s) 121
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 3 cut(s) 162, 322, 594
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 3 cut(s) 43, 531, 577
BstKTI GATC 5 cut(s) 262, 277, 537, 652, 754
BstMAI GTCTC 1 cut(s) 215
BstMBI GATC 5 cut(s) 259, 274, 534, 649, 751
BstMWI GCNNNNNNNGC 5 cut(s) 80, 83, 92, 215, 301
BstV1I GCAGC 4 cut(s) 58, 61, 196, 282
BstV2I GAAGAC 2 cut(s) 519, 536
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 2 cut(s) 83, 716
BtgI CCRYGG 1 cut(s) 78
BtsCI GGATG 3 cut(s) 43, 531, 577
BveI ACCTGC 1 cut(s) 42
Cac8I GCNNGC 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 667
Csp6I GTAC 2 cut(s) 478, 560
CspCI CAANNNNNGTGG 2 cut(s) 423, 458
CviAII CATG 2 cut(s) 79, 367
CviQI GTAC 2 cut(s) 478, 560
DdeI CTNAG 3 cut(s) 162, 322, 594
DpnI GATC 5 cut(s) 261, 276, 536, 651, 753
DpnII GATC 5 cut(s) 259, 274, 534, 649, 751
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 413, 468
EarI CTCTTC 2 cut(s) 413, 468
EciI GGCGGA 2 cut(s) 126, 233
Eco130I CCWWGG 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 78
ErhI CCWWGG 1 cut(s) 78
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 2 cut(s) 82, 370
FaiI YATR 7 cut(s) 80, 98, 368, 539, 586, 588, 762
FalI AAGNNNNNCTT 2 cut(s) 115, 147
FatI CATG 2 cut(s) 78, 366
FbaI TGATCA 1 cut(s) 751
Fnu4HI GCNGC 5 cut(s) 72, 75, 84, 210, 296
FokI GGATG 3 cut(s) 30, 538, 584
Fsp4HI GCNGC 5 cut(s) 72, 75, 84, 210, 296
FspBI CTAG 1 cut(s) 542
GluI GCNGC 5 cut(s) 72, 75, 84, 210, 296
HaeIII GGCC 2 cut(s) 83, 716
HapII CCGG 4 cut(s) 354, 509, 611, 665
Hin1II CATG 2 cut(s) 82, 370
HindIII AAGCTT 1 cut(s) 439
HinfI GANTC 4 cut(s) 149, 176, 515, 607
HpaII CCGG 4 cut(s) 354, 509, 611, 665
HphI GGTGA 1 cut(s) 557
Hpy188I TCNGA 5 cut(s) 148, 181, 205, 397, 654
Hpy188III TCNNGA 7 cut(s) 263, 324, 339, 412, 523, 710, 755
HpyAV CCTTC 3 cut(s) 153, 396, 652
HpyCH4V TGCA 3 cut(s) 189, 196, 212
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 83, 92, 215, 301
HpyF3I CTNAG 3 cut(s) 162, 322, 594
Hsp92II CATG 2 cut(s) 82, 370
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 5 cut(s) 259, 274, 534, 649, 751
LguI GCTCTTC 1 cut(s) 413
Lsp1109I GCAGC 4 cut(s) 58, 61, 196, 282
LweI GCATC 2 cut(s) 172, 580
MaeI CTAG 1 cut(s) 542
MalI GATC 5 cut(s) 261, 276, 536, 651, 753
MboI GATC 5 cut(s) 259, 274, 534, 649, 751
MboII GAAGA 9 cut(s) 18, 21, 123, 135, 328, 400, 485, 524, 541
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 1 cut(s) 723
MluCI AATT 6 cut(s) 12, 377, 415, 428, 702, 775
MlyI GAGTC 1 cut(s) 509
MroXI GAANNNNTTC 2 cut(s) 13, 130
MseI TTAA 4 cut(s) 426, 468, 705, 778
MslI CAYNNNNRTG 1 cut(s) 765
MspI CCGG 4 cut(s) 354, 509, 611, 665
MwoI GCNNNNNNNGC 5 cut(s) 80, 83, 92, 215, 301
NcoI CCATGG 1 cut(s) 78
NdeII GATC 5 cut(s) 259, 274, 534, 649, 751
NlaIII CATG 2 cut(s) 82, 370
NlaIV GGNNCC 1 cut(s) 276
NmeAIII GCCGAG 1 cut(s) 742
NspV TTCGAA 1 cut(s) 121
PciSI GCTCTTC 1 cut(s) 413
PdmI GAANNNNTTC 2 cut(s) 13, 130
PfeI GAWTC 3 cut(s) 149, 176, 607
PkrI GCNGC 5 cut(s) 73, 76, 85, 211, 297
PleI GAGTC 1 cut(s) 509
PpsI GAGTC 1 cut(s) 509
PshBI ATTAAT 1 cut(s) 705
PspN4I GGNNCC 1 cut(s) 276
PspPI GGNCC 1 cut(s) 667
PsuI RGATCY 1 cut(s) 274
RsaI GTAC 2 cut(s) 479, 561
RsaNI GTAC 2 cut(s) 478, 560
RseI CAYNNNNRTG 1 cut(s) 765
SapI GCTCTTC 1 cut(s) 413
SaqAI TTAA 4 cut(s) 426, 468, 705, 778
SatI GCNGC 5 cut(s) 72, 75, 84, 210, 296
Sau3AI GATC 5 cut(s) 259, 274, 534, 649, 751
Sau96I GGNCC 1 cut(s) 667
SchI GAGTC 1 cut(s) 509
SduI GDGCHC 1 cut(s) 723
SfaNI GCATC 2 cut(s) 172, 580
SfuI TTCGAA 1 cut(s) 121
SinI GGWCC 1 cut(s) 667
SmiMI CAYNNNNRTG 1 cut(s) 765
Sse9I AATT 6 cut(s) 12, 377, 415, 428, 702, 775
SsiI CCGC 3 cut(s) 84, 137, 218
SspMI CTAG 1 cut(s) 542
StyI CCWWGG 1 cut(s) 78
TaqI TCGA 4 cut(s) 121, 363, 413, 446
TasI AATT 6 cut(s) 12, 377, 415, 428, 702, 775
TatI WGTACW 1 cut(s) 477
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 3 cut(s) 149, 176, 607
Tru1I TTAA 4 cut(s) 426, 468, 705, 778
Tru9I TTAA 4 cut(s) 426, 468, 705, 778
TseI GCWGC 4 cut(s) 71, 74, 209, 295
TspDTI ATGAA 6 cut(s) 329, 383, 542, 588, 634, 692
TspGWI ACGGA 2 cut(s) 36, 510
VpaK11BI GGWCC 1 cut(s) 667
VspI ATTAAT 1 cut(s) 705
XapI RAATTY 2 cut(s) 12, 415
XmnI GAANNNNTTC 2 cut(s) 13, 130
XspI CTAG 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.