pycom08g05640
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013969
Physical Location & Seq
Reverse (-)
4270239 .. 4271148
910 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05640.1

Sequence Viewer

Length: 489 bp
ATGTCTGGTGGCAACTTCAGGTCAGTGGAGTCACCTGAGACTAATGACTTTTCCAGCAACTTGAATTTTGAGTTTTCGGAGTACTTGATGATCCGCAAGTGGCTGGATGAGATCGATCCGAGTTTCGTAGGTTCGGAGTTTTCTGTCCAGAATTCGGGTTTTCGAGCGAATGAAGTTGGTGAGTCTGGTGCAAGAAGCAGCCAACCTGGAGGGTCTACTAGCAGAGGAGAGAATGGAAGCAGCCGGAAGAGGCAGGAAGCTGGAGACAGAGTTGCATTCAAAACAAAATCAGAGGTTGAAATATTGGATGATGGGTTCAGGTGGAGGAAGTATGGTAAAAAGATGGTGAAAAGCAGCCCTAATCCAAGAAATTACTACAAGTGTTCAATTGGAGGGTGCCACGTGAAAAAAAGAGTTGAGAGAGATAGGGAGGATCCAAGGTTTGTAATTACAACTTATGATGGAGTCCATAATCATCCAGGCCTCTAA

Protein Analysis

163

Amino Acids

18.3

Weight (kDa)

8.88

Isoelectric Point (pI)

55.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 103 - 160 1.1e-24 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013969)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26170
fragaria_vesca FvH4_2g31400 FvH4_2g31400
malus_domestica MD08G1067700.v1.1 MD15G1054000.v1.1
prunus_persica Prupe.1G407500_v2.0.a1
pyrus_communis pycom08g05640 pycom15g05090
rosa_chinensis RchiOBHm_Chr6g0311421
rosa_laevigata RLG00000010408
rosa_roxburghii Rroxscaffold_7G00157630
rosa_rugosa Rorug06G0389000
rosa_samantha Rh6AG503200 Rh6BG513800 Rh6CG519600 Rh6DG505800
rosa_wichuraiana Rw6G043820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 396
AccI GTMKAC 1 cut(s) 215
AciI CCGC 1 cut(s) 94
AclWI GGATC 4 cut(s) 85, 110, 428, 441
AcsI RAATTY 2 cut(s) 64, 151
AcvI CACGTG 1 cut(s) 403
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 154
AgsI TTSAA 4 cut(s) 64, 280, 299, 387
AjnI CCWGG 2 cut(s) 205, 478
AloI GAACNNNNNNTCC 2 cut(s) 299, 331
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
Alw26I GTCTC 2 cut(s) 32, 258
AlwI GGATC 4 cut(s) 85, 110, 428, 441
AoxI GGCC 1 cut(s) 481
ApeKI GCWGC 3 cut(s) 198, 240, 354
ApoI RAATTY 2 cut(s) 64, 151
AsuHPI GGTGA 3 cut(s) 24, 191, 358
BamHI GGATCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 396
BbrPI CACGTG 1 cut(s) 403
BbvI GCAGC 3 cut(s) 210, 252, 366
BccI CCATC 3 cut(s) 305, 337, 455
BciT130I CCWGG 2 cut(s) 207, 480
BcoDI GTCTC 2 cut(s) 32, 258
BfaI CTAG 1 cut(s) 219
BisI GCNGC 3 cut(s) 199, 241, 355
BlsI GCNGC 3 cut(s) 200, 242, 356
BmcAI AGTACT 1 cut(s) 83
Bme1390I CCNGG 2 cut(s) 207, 480
BmiI GGNNCC 2 cut(s) 398, 435
BmrFI CCNGG 2 cut(s) 207, 480
BpmI CTGGAG 2 cut(s) 228, 282
Bsa29I ATCGAT 1 cut(s) 114
BsaAI YACGTR 1 cut(s) 403
BsaJI CCNNGG 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 154
BseBI CCWGG 2 cut(s) 207, 480
BseCI ATCGAT 1 cut(s) 114
BseDI CCNNGG 1 cut(s) 437
BseGI GGATG 3 cut(s) 112, 313, 475
BseLI CCNNNNNNNGG 1 cut(s) 154
BseMII CTCAG 1 cut(s) 27
BseRI GAGGAG 1 cut(s) 240
BseXI GCAGC 3 cut(s) 210, 252, 366
BshFI GGCC 1 cut(s) 483
BshNI GGYRCC 1 cut(s) 396
BshVI ATCGAT 1 cut(s) 114
BsiSI CCGG 1 cut(s) 244
BslI CCNNNNNNNGG 1 cut(s) 154
BsmAI GTCTC 2 cut(s) 32, 258
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 1 cut(s) 483
Bsp143I GATC 4 cut(s) 90, 111, 115, 433
BspACI CCGC 1 cut(s) 94
BspANI GGCC 1 cut(s) 483
BspCNI CTCAG 1 cut(s) 28
BspDI ATCGAT 1 cut(s) 114
BspLI GGNNCC 2 cut(s) 398, 435
BspPI GGATC 4 cut(s) 85, 110, 428, 441
BspT107I GGYRCC 1 cut(s) 396
BssECI CCNNGG 1 cut(s) 437
BssMI GATC 4 cut(s) 90, 111, 115, 433
BssT1I CCWWGG 1 cut(s) 437
Bst2UI CCWGG 2 cut(s) 207, 480
Bst6I CTCTTC 1 cut(s) 242
BstBAI YACGTR 1 cut(s) 403
BstDEI CTNAG 1 cut(s) 36
BstF5I GGATG 3 cut(s) 112, 313, 475
BstKTI GATC 4 cut(s) 93, 114, 118, 436
BstMAI GTCTC 2 cut(s) 32, 258
BstMBI GATC 4 cut(s) 90, 111, 115, 433
BstNI CCWGG 2 cut(s) 207, 480
BstSCI CCNGG 2 cut(s) 205, 478
BstV1I GCAGC 3 cut(s) 210, 252, 366
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
Bsu15I ATCGAT 1 cut(s) 114
BsuRI GGCC 1 cut(s) 483
BsuTUI ATCGAT 1 cut(s) 114
BtsCI GGATG 3 cut(s) 112, 313, 475
BtsIMutI CAGTG 1 cut(s) 30
ClaI ATCGAT 1 cut(s) 114
Csp6I GTAC 1 cut(s) 82
CviJI RGCY 6 cut(s) 103, 201, 243, 260, 357, 483
CviKI_1 RGCY 6 cut(s) 103, 201, 243, 260, 357, 483
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 1 cut(s) 36
DpnI GATC 4 cut(s) 92, 113, 117, 435
DpnII GATC 4 cut(s) 90, 111, 115, 433
Eam1104I CTCTTC 1 cut(s) 242
EarI CTCTTC 1 cut(s) 242
Eco130I CCWWGG 1 cut(s) 437
Eco147I AGGCCT 1 cut(s) 483
Eco72I CACGTG 1 cut(s) 403
EcoRI GAATTC 1 cut(s) 151
EcoRII CCWGG 2 cut(s) 205, 478
EcoT14I CCWWGG 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 437
FaiI YATR 3 cut(s) 333, 459, 471
FblI GTMKAC 1 cut(s) 215
Fnu4HI GCNGC 3 cut(s) 199, 241, 355
FokI GGATG 3 cut(s) 119, 320, 462
Fsp4HI GCNGC 3 cut(s) 199, 241, 355
FspBI CTAG 1 cut(s) 219
GluI GCNGC 3 cut(s) 199, 241, 355
GsuI CTGGAG 2 cut(s) 228, 282
HaeIII GGCC 1 cut(s) 483
HapII CCGG 1 cut(s) 244
HinfI GANTC 3 cut(s) 29, 182, 465
HpaII CCGG 1 cut(s) 244
HphI GGTGA 3 cut(s) 24, 191, 358
Hpy166II GTNNAC 1 cut(s) 216
Hpy188I TCNGA 4 cut(s) 79, 120, 136, 292
Hpy188III TCNNGA 1 cut(s) 148
Hpy8I GTNNAC 1 cut(s) 216
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 2 cut(s) 191, 275
HpyF3I CTNAG 1 cut(s) 36
HpySE526I ACGT 1 cut(s) 402
Kzo9I GATC 4 cut(s) 90, 111, 115, 433
Lsp1109I GCAGC 3 cut(s) 210, 252, 366
MaeI CTAG 1 cut(s) 219
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 30
MalI GATC 4 cut(s) 92, 113, 117, 435
MboI GATC 4 cut(s) 90, 111, 115, 433
MboII GAAGA 1 cut(s) 259
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 1 cut(s) 433
MluCI AATT 5 cut(s) 64, 151, 370, 387, 447
MlyI GAGTC 3 cut(s) 38, 191, 474
MnlI CCTC 7 cut(s) 203, 218, 243, 286, 318, 386, 424
MspI CCGG 1 cut(s) 244
MspR9I CCNGG 2 cut(s) 207, 480
MunI CAATTG 1 cut(s) 387
Mva1269I GAATGC 1 cut(s) 275
MvaI CCWGG 2 cut(s) 207, 480
NdeII GATC 4 cut(s) 90, 111, 115, 433
NlaIV GGNNCC 2 cut(s) 398, 435
NmuCI GTSAC 1 cut(s) 30
PceI AGGCCT 1 cut(s) 483
PctI GAATGC 1 cut(s) 275
PkrI GCNGC 3 cut(s) 200, 242, 356
PleI GAGTC 3 cut(s) 37, 190, 473
PmaCI CACGTG 1 cut(s) 403
PmlI CACGTG 1 cut(s) 403
PpsI GAGTC 3 cut(s) 37, 190, 473
Ppu21I YACGTR 1 cut(s) 403
Psp6I CCWGG 2 cut(s) 205, 478
PspCI CACGTG 1 cut(s) 403
PspGI CCWGG 2 cut(s) 205, 478
PspN4I GGNNCC 2 cut(s) 398, 435
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
SatI GCNGC 3 cut(s) 199, 241, 355
Sau3AI GATC 4 cut(s) 90, 111, 115, 433
ScaI AGTACT 1 cut(s) 83
SchI GAGTC 3 cut(s) 38, 191, 474
ScrFI CCNGG 2 cut(s) 207, 480
SetI ASST 9 cut(s) 23, 37, 133, 208, 262, 297, 323, 405, 443
Sse9I AATT 5 cut(s) 64, 151, 370, 387, 447
SseBI AGGCCT 1 cut(s) 483
SsiI CCGC 1 cut(s) 94
SspI AATATT 1 cut(s) 303
SspMI CTAG 1 cut(s) 219
StuI AGGCCT 1 cut(s) 483
StyD4I CCNGG 2 cut(s) 205, 478
StyI CCWWGG 1 cut(s) 437
TaiI ACGT 1 cut(s) 405
TaqI TCGA 2 cut(s) 114, 163
TasI AATT 5 cut(s) 64, 151, 370, 387, 447
TatI WGTACW 1 cut(s) 81
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 1 cut(s) 30
TseI GCWGC 3 cut(s) 198, 240, 354
Tsp45I GTSAC 1 cut(s) 30
TspDTI ATGAA 1 cut(s) 186
TspRI CASTG 1 cut(s) 30
XapI RAATTY 2 cut(s) 64, 151
XmiI GTMKAC 1 cut(s) 215
XspI CTAG 1 cut(s) 219
ZrmI AGTACT 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.