RLG00000010408
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013969
Physical Location & Seq
Forward (+)
1390772 .. 1391411
640 bp
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UTR
Exon/CDS
Intron
RLM00000010408

Sequence Viewer

Length: 471 bp
ATGTCGAATAACAACTTCAACAGATCAGTGCAGACACCTGAGAATGACTTTTCCGACCAACCAAATTTCGACGAGTACTTGATGTTTGGTGACTGGCTTGATGAAGATCATAACTATGTAGCTTCAGGGTCTACTCAGAATTCGGTTAACCAAGTAAATGATGATGATTCTGGTGGAAGTAGCAGCCAACTTGGAGGTTCTACTACCAATGAGAAAGGAAGCGTACTGGAGAGGCAGCAAGTTAAAGAAAGAGTCGCCTTCAAAATGAAATCAGAGGTTGAGATTTTAGATGATGGGTTCAAATGGAGGAAGTATGGGAAAAAGATGGTGAAGAACAGCCCAAATCCAAGGAATTACTACAAATGTTCATGTGAAGGCTGCCCAGTGAAAAAGAGAGTTGAAAGAGATAGAGAAGATCCAGCGTTTGTAATTACGACTTACGAAGGCACTCATAACCATCGGAGCGTCTGA

Protein Analysis

157

Amino Acids

17.85

Weight (kDa)

5.43

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 97 - 153 6.2e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013969)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G26170
fragaria_vesca FvH4_2g31400 FvH4_2g31400
malus_domestica MD08G1067700.v1.1 MD15G1054000.v1.1
prunus_persica Prupe.1G407500_v2.0.a1
pyrus_communis pycom08g05640 pycom15g05090
rosa_chinensis RchiOBHm_Chr6g0311421
rosa_laevigata RLG00000010408
rosa_roxburghii Rroxscaffold_7G00157630
rosa_rugosa Rorug06G0389000
rosa_samantha Rh6AG503200 Rh6BG513800 Rh6CG519600 Rh6DG505800
rosa_wichuraiana Rw6G043820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 131
AclWI GGATC 1 cut(s) 410
AcsI RAATTY 2 cut(s) 64, 139
AcuI CTGAAG 1 cut(s) 108
AfaI GTAC 2 cut(s) 77, 225
AgsI TTSAA 4 cut(s) 19, 262, 301, 401
AluBI AGCT 1 cut(s) 122
AluI AGCT 1 cut(s) 122
AlwI GGATC 1 cut(s) 410
ApeKI GCWGC 3 cut(s) 183, 235, 378
ApoI RAATTY 2 cut(s) 64, 139
AsuHPI GGTGA 2 cut(s) 101, 340
BbvI GCAGC 3 cut(s) 195, 247, 365
BccI CCATC 3 cut(s) 287, 319, 465
BisI GCNGC 3 cut(s) 184, 236, 379
BlsI GCNGC 3 cut(s) 185, 237, 380
BmcAI AGTACT 1 cut(s) 77
BmrI ACTGGG 1 cut(s) 377
BmuI ACTGGG 1 cut(s) 377
BpmI CTGGAG 1 cut(s) 248
BsaBI GATNNNNATC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 347
Bse1I ACTGG 3 cut(s) 98, 231, 383
Bse8I GATNNNNATC 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 347
BseJI GATNNNNATC 1 cut(s) 105
BseMII CTCAG 2 cut(s) 30, 149
BseNI ACTGG 3 cut(s) 98, 231, 383
BseXI GCAGC 3 cut(s) 195, 247, 365
BsgI GTGCAG 1 cut(s) 50
Bsp143I GATC 3 cut(s) 23, 106, 415
BspCNI CTCAG 2 cut(s) 31, 148
BspPI GGATC 1 cut(s) 410
BsrI ACTGG 3 cut(s) 98, 231, 383
BssECI CCNNGG 1 cut(s) 347
BssMI GATC 3 cut(s) 23, 106, 415
BssT1I CCWWGG 1 cut(s) 347
BstDEI CTNAG 2 cut(s) 39, 135
BstKTI GATC 3 cut(s) 26, 109, 418
BstMBI GATC 3 cut(s) 23, 106, 415
BstV1I GCAGC 3 cut(s) 195, 247, 365
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BtsIMutI CAGTG 2 cut(s) 33, 390
CseI GACGC 1 cut(s) 454
Csp6I GTAC 2 cut(s) 76, 224
CviAII CATG 1 cut(s) 369
CviJI RGCY 5 cut(s) 97, 122, 186, 339, 378
CviKI_1 RGCY 5 cut(s) 97, 122, 186, 339, 378
CviQI GTAC 2 cut(s) 76, 224
DdeI CTNAG 2 cut(s) 39, 135
DpnI GATC 3 cut(s) 25, 108, 417
DpnII GATC 3 cut(s) 23, 106, 415
Eco130I CCWWGG 1 cut(s) 347
Eco57I CTGAAG 1 cut(s) 108
EcoRI GAATTC 1 cut(s) 139
EcoT14I CCWWGG 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 347
FaeI CATG 1 cut(s) 372
FaiI YATR 5 cut(s) 111, 117, 315, 370, 453
FatI CATG 1 cut(s) 368
FblI GTMKAC 1 cut(s) 131
Fnu4HI GCNGC 3 cut(s) 184, 236, 379
Fsp4HI GCNGC 3 cut(s) 184, 236, 379
GluI GCNGC 3 cut(s) 184, 236, 379
GsuI CTGGAG 1 cut(s) 248
HgaI GACGC 1 cut(s) 454
Hin1II CATG 1 cut(s) 372
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 2 cut(s) 167, 252
HpaI GTTAAC 1 cut(s) 148
HphI GGTGA 2 cut(s) 101, 340
Hpy166II GTNNAC 2 cut(s) 132, 148
Hpy188I TCNGA 5 cut(s) 55, 138, 274, 462, 470
Hpy8I GTNNAC 2 cut(s) 132, 148
Hpy99I CGWCG 1 cut(s) 74
HpyAV CCTTC 3 cut(s) 268, 368, 437
HpyCH4V TGCA 1 cut(s) 31
HpyF3I CTNAG 2 cut(s) 39, 135
Hsp92II CATG 1 cut(s) 372
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 3 cut(s) 23, 106, 415
LmnI GCTCC 1 cut(s) 462
LpnPI CCDG 7 cut(s) 51, 79, 111, 156, 212, 396, 432
Lsp1109I GCAGC 3 cut(s) 195, 247, 365
MaeIII GTNAC 1 cut(s) 89
MalI GATC 3 cut(s) 25, 108, 417
MboI GATC 3 cut(s) 23, 106, 415
MboII GAAGA 3 cut(s) 116, 343, 425
MflI RGATCY 1 cut(s) 415
MluCI AATT 4 cut(s) 64, 139, 352, 429
MlyI GAGTC 1 cut(s) 261
MmeI TCCRAC 1 cut(s) 78
MnlI CCTC 4 cut(s) 188, 225, 268, 300
MseI TTAA 2 cut(s) 147, 243
MslI CAYNNNNRTG 1 cut(s) 114
NdeII GATC 3 cut(s) 23, 106, 415
NlaIII CATG 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 89
PfeI GAWTC 1 cut(s) 167
PkrI GCNGC 3 cut(s) 185, 237, 380
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 2 cut(s) 77, 225
RsaNI GTAC 2 cut(s) 76, 224
RseI CAYNNNNRTG 1 cut(s) 114
SaqAI TTAA 2 cut(s) 147, 243
SatI GCNGC 3 cut(s) 184, 236, 379
Sau3AI GATC 3 cut(s) 23, 106, 415
ScaI AGTACT 1 cut(s) 77
SchI GAGTC 1 cut(s) 261
SetI ASST 4 cut(s) 40, 124, 199, 279
SmiMI CAYNNNNRTG 1 cut(s) 114
Sse9I AATT 4 cut(s) 64, 139, 352, 429
StyI CCWWGG 1 cut(s) 347
TaqI TCGA 2 cut(s) 5, 69
TasI AATT 4 cut(s) 64, 139, 352, 429
TatI WGTACW 1 cut(s) 75
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 2 cut(s) 147, 243
Tru9I TTAA 2 cut(s) 147, 243
TscAI CASTG 2 cut(s) 33, 390
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 3 cut(s) 183, 235, 378
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 3 cut(s) 117, 281, 357
TspRI CASTG 2 cut(s) 33, 390
XapI RAATTY 2 cut(s) 64, 139
XmiI GTMKAC 1 cut(s) 131
ZrmI AGTACT 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.