pycom08g05830

Peroxisome biogenesis factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0005456
Physical Location & Seq
Reverse (-)
4388564 .. 4391449
2886 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1074 bp
ATGGGTTGGAACGAGGCACCTGGGTCGGGGCAGCAAACCGGCCCGAGCGGGGGAGAACCGGGTCCAAGCAGCATCAGCAGCGACCCGGGCGCGGCACGGCGGTTCCCGCCGGCGGCTCAGCCGGAGATCATGAGAGCCGCCGAAAAGGACGACCAGTACGCCTCCTTCGTCTACGACGCTTGCCGCGACGCCGTCCGCCACCTCTTGGGGTCCGGCCGGCAAACACTTGGGGAAGAGTATTGCGACATCACTCAGGTTGCAGGACCTTATGGACTGCCTCCGACCCCGGCAAGCTCTTCCTTATATTGCAGACAGCTCTTCCTTGTGTACCAGACAGCTCTTCCTTATATTGCAGAACGAATTAGTGCTTCATTGAGCAATGATGCTCAATCTTCTTCAACTATTCATGCTTCAACTGCCTCTACATCTGGGCCATCTGTTTCAGCTTTGAGAAGATTAAAAGAGAAGTTCAACGGGTTATGGATTTCGTTAGTCCGGCGATGGCCTACGGTGCTTCCTATAGCTCGAGAATTTTTGCAATTGGTTATTCGTGCTAACCTCATGTTCTTTTACTTTGAAGGCCTTTTCTACCATATATCAAAACGAACAGCAGGTATTCGTTATGTGTTCATCGGAAAACCATCTAACCAAAGGCCCAGATACCAAATTTTAGGCGTATTTCTATTGATTCAGCTTTGTATCATAGCTGCTGAAGGACTGAGGCGCAGCAATTTATCCTCTATTTCAAGTTCAGCTCGTCAGACTTTTGGTACACACCAAACTTCTGCAGGTCGAGATTTGCCTGTGTTAAATGAGGAAGGTAATTTGATATCTGTGGATGCTGCCAAGGGAAGTTGGGTCTCTGAATCTTCAGCTTCAGAGTCTCAGGCCAGCGGGACCAGCAAATGCACACTCTGTTTGAGTAATCGCCAATACCCAACAGCCACACCTTGCGGTCACGTATTTTGCTGGAGCTGCATTATGGAATGGTGCAATGAAAAGCCAGAATGCCCTCTCTGCCGGACTCCCATAACTCATTCGAGCCTAGTTTGCTTGTACCATTCAGATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005829 GO:0006082 GO:0006464 GO:0006513 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009790 GO:0009791 GO:0009793 GO:0009853 GO:0009987 GO:0010154 GO:0010381 GO:0015031 GO:0015833 GO:0015919 GO:0016020 GO:0016042 GO:0016043 GO:0016054 GO:0016558 GO:0016567 GO:0016740 GO:0017038 GO:0019395 GO:0019538 GO:0019752 GO:0019787 GO:0022406 GO:0022414 GO:0030258 GO:0031090 GO:0031903 GO:0032446 GO:0032501 GO:0032502 GO:0032787 GO:0033036 GO:0033365 GO:0034440 GO:0034613 GO:0036211 GO:0042579 GO:0042886 GO:0043094 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046907 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0055085 GO:0055114 GO:0061458 GO:0065002 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0098588 GO:0098805 GO:0140056 GO:0140096 GO:1901564 GO:1901575
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
Pex2_Pex12 zf-C3HC4_3 zf-RING_2

Protein Analysis

358

Amino Acids

39.03

Weight (kDa)

8.39

Isoelectric Point (pI)

56.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pex2_Pex12 PF04757 66 - 241 1.4e-23 Pex2 / Pex12 amino terminal region
zf-C3HC4_3 PF13920 301 - 346 5.4e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 302 - 341 3.2e-10 Ring finger domain
zf-C3HC4_2 PF13923 302 - 340 2e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 303 - 340 2.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 303 - 338 6.7e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 602, 779
AccB1I GGYRCC 1 cut(s) 16
AccB7I CCANNNNNTGG 1 cut(s) 205
AccBSI CCGCTC 1 cut(s) 48
AccI GTMKAC 1 cut(s) 171
AccII CGCG 2 cut(s) 92, 186
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 2 cut(s) 530, 666
AcuI CTGAAG 3 cut(s) 732, 855, 861
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 4 cut(s) 158, 329, 772, 1058
AfiI CCNNNNNNNGG 6 cut(s) 26, 49, 50, 91, 112, 205
AgsI TTSAA 5 cut(s) 399, 414, 472, 578, 747
AjnI CCWGG 1 cut(s) 19
Alw26I GTCTC 2 cut(s) 865, 888
Ama87I CYCGRG 3 cut(s) 43, 85, 525
AoxI GGCC 7 cut(s) 40, 214, 431, 503, 580, 653, 888
ApeKI GCWGC 7 cut(s) 31, 69, 78, 707, 726, 842, 975
ApoI RAATTY 2 cut(s) 530, 666
AspLEI GCGC 2 cut(s) 92, 726
AspS9I GGNCC 7 cut(s) 41, 62, 210, 263, 431, 654, 897
AsuC2I CCSGG 4 cut(s) 60, 86, 87, 287
AvaI CYCGRG 3 cut(s) 43, 85, 525
AvaII GGWCC 4 cut(s) 62, 210, 263, 897
BanI GGYRCC 1 cut(s) 16
BarI GAAGNNNNNNTAC 2 cut(s) 149, 181
BbvI GCAGC 7 cut(s) 43, 81, 90, 694, 738, 829, 962
BccI CCATC 3 cut(s) 442, 495, 649
BceAI ACGGC 2 cut(s) 113, 176
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BciT130I CCWGG 1 cut(s) 21
BcnI CCSGG 4 cut(s) 60, 86, 87, 287
BcoDI GTCTC 2 cut(s) 865, 888
BfaI CTAG 2 cut(s) 1046, 1072
BfmI CTRYAG 2 cut(s) 519, 786
BfuAI ACCTGC 2 cut(s) 602, 779
BlpI GCTNAGC 1 cut(s) 117
Bme1390I CCNGG 5 cut(s) 21, 60, 86, 87, 287
Bme18I GGWCC 4 cut(s) 62, 210, 263, 897
BmeT110I CYCGRG 3 cut(s) 43, 85, 525
BmgT120I GGNCC 7 cut(s) 41, 62, 210, 263, 431, 654, 897
BmiI GGNNCC 5 cut(s) 18, 63, 104, 211, 898
BmrFI CCNGG 5 cut(s) 21, 60, 86, 87, 287
BmsI GCATC 3 cut(s) 81, 373, 829
BpmI CTGGAG 1 cut(s) 991
Bpu1102I GCTNAGC 1 cut(s) 117
BpuMI CCSGG 4 cut(s) 60, 86, 87, 287
BsaAI YACGTR 1 cut(s) 961
BsaHI GRCGYC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 865
BsaJI CCNNGG 4 cut(s) 20, 85, 285, 846
Bsc4I CCNNNNNNNGG 6 cut(s) 26, 49, 50, 91, 112, 205
Bse118I RCCGGY 3 cut(s) 38, 109, 216
Bse1I ACTGG 1 cut(s) 154
Bse3DI GCAATG 2 cut(s) 385, 1000
BseBI CCWGG 1 cut(s) 21
BseDI CCNNGG 4 cut(s) 20, 85, 285, 846
BseGI GGATG 1 cut(s) 844
BseLI CCNNNNNNNGG 6 cut(s) 26, 49, 50, 91, 112, 205
BseMI GCAATG 2 cut(s) 385, 1000
BseMII CTCAG 4 cut(s) 131, 266, 710, 899
BseNI ACTGG 1 cut(s) 154
BseX3I CGGCCG 1 cut(s) 214
BseXI GCAGC 7 cut(s) 43, 81, 90, 694, 738, 829, 962
Bsh1236I CGCG 2 cut(s) 92, 186
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 7 cut(s) 42, 216, 433, 505, 582, 655, 890
BshNI GGYRCC 1 cut(s) 16
BsiEI CGRYCG 1 cut(s) 217
BsiHKCI CYCGRG 3 cut(s) 43, 85, 525
BslFI GGGAC 1 cut(s) 910
BslI CCNNNNNNNGG 6 cut(s) 26, 49, 50, 91, 112, 205
BsmAI GTCTC 2 cut(s) 865, 888
BsmFI GGGAC 1 cut(s) 910
BsmI GAATGC 1 cut(s) 1013
BsnI GGCC 7 cut(s) 42, 216, 433, 505, 582, 655, 890
Bso31I GGTCTC 1 cut(s) 865
BsoBI CYCGRG 3 cut(s) 43, 85, 525
Bsp143I GATC 1 cut(s) 126
Bsp1720I GCTNAGC 1 cut(s) 117
BspANI GGCC 7 cut(s) 42, 216, 433, 505, 582, 655, 890
BspCNI CTCAG 4 cut(s) 130, 265, 711, 898
BspFNI CGCG 2 cut(s) 92, 186
BspHI TCATGA 1 cut(s) 129
BspLI GGNNCC 5 cut(s) 18, 63, 104, 211, 898
BspMAI CTGCAG 1 cut(s) 790
BspMI ACCTGC 2 cut(s) 602, 779
BspQI GCTCTTC 3 cut(s) 301, 323, 345
BspT107I GGYRCC 1 cut(s) 16
BspTNI GGTCTC 1 cut(s) 865
BsrBI CCGCTC 1 cut(s) 48
BsrDI GCAATG 2 cut(s) 385, 1000
BsrFI RCCGGY 3 cut(s) 38, 109, 216
BsrI ACTGG 1 cut(s) 154
BssAI RCCGGY 3 cut(s) 38, 109, 216
BssECI CCNNGG 4 cut(s) 20, 85, 285, 846
BssMI GATC 1 cut(s) 126
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 846
Bst2UI CCWGG 1 cut(s) 21
Bst4CI ACNGT 1 cut(s) 511
Bst6I CTCTTC 4 cut(s) 228, 301, 323, 345
BstACI GRCGYC 1 cut(s) 189
BstBAI YACGTR 1 cut(s) 961
BstC8I GCNNGC 5 cut(s) 111, 181, 218, 292, 892
BstDEI CTNAG 4 cut(s) 117, 252, 719, 885
BstF5I GGATG 1 cut(s) 844
BstFNI CGCG 2 cut(s) 92, 186
BstHHI GCGC 2 cut(s) 92, 726
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 2 cut(s) 865, 888
BstMBI GATC 1 cut(s) 126
BstMCI CGRYCG 1 cut(s) 217
BstNI CCWGG 1 cut(s) 21
BstSCI CCNGG 5 cut(s) 19, 58, 84, 85, 285
BstSFI CTRYAG 2 cut(s) 519, 786
BstUI CGCG 2 cut(s) 92, 186
BstV1I GCAGC 7 cut(s) 43, 81, 90, 694, 738, 829, 962
BstZI CGGCCG 1 cut(s) 214
BsuRI GGCC 7 cut(s) 42, 216, 433, 505, 582, 655, 890
BtgZI GCGATG 1 cut(s) 514
BtsCI GGATG 1 cut(s) 844
BveI ACCTGC 2 cut(s) 602, 779
Cac8I GCNNGC 5 cut(s) 111, 181, 218, 292, 892
CciI TCATGA 1 cut(s) 129
CfoI GCGC 2 cut(s) 92, 726
Cfr10I RCCGGY 3 cut(s) 38, 109, 216
Cfr13I GGNCC 7 cut(s) 41, 62, 210, 263, 431, 654, 897
Cfr9I CCCGGG 1 cut(s) 85
CseI GACGC 2 cut(s) 185, 197
Csp6I GTAC 4 cut(s) 157, 328, 771, 1057
CviAII CATG 3 cut(s) 130, 407, 562
CviQI GTAC 4 cut(s) 157, 328, 771, 1057
DdeI CTNAG 4 cut(s) 117, 252, 719, 885
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
EaeI YGGCCR 1 cut(s) 214
EagI CGGCCG 1 cut(s) 214
Eam1104I CTCTTC 4 cut(s) 228, 301, 323, 345
EarI CTCTTC 4 cut(s) 228, 301, 323, 345
EciI GGCGGA 1 cut(s) 185
EclXI CGGCCG 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 846
Eco147I AGGCCT 1 cut(s) 582
Eco31I GGTCTC 1 cut(s) 865
Eco32I GATATC 1 cut(s) 831
Eco47I GGWCC 4 cut(s) 62, 210, 263, 897
Eco52I CGGCCG 1 cut(s) 214
Eco57I CTGAAG 3 cut(s) 732, 855, 861
Eco88I CYCGRG 3 cut(s) 43, 85, 525
EcoO109I RGGNCCY 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 19
EcoRV GATATC 1 cut(s) 831
EcoT14I CCWWGG 1 cut(s) 846
ErhI CCWWGG 1 cut(s) 846
FaeI CATG 3 cut(s) 133, 410, 565
FaqI GGGAC 1 cut(s) 910
FatI CATG 3 cut(s) 129, 406, 561
FauI CCCGC 3 cut(s) 41, 114, 887
FblI GTMKAC 1 cut(s) 171
FokI GGATG 1 cut(s) 851
FspBI CTAG 2 cut(s) 1046, 1072
GlaI GCGC 2 cut(s) 91, 725
GsuI CTGGAG 1 cut(s) 991
HaeIII GGCC 7 cut(s) 42, 216, 433, 505, 582, 655, 890
HgaI GACGC 2 cut(s) 185, 197
HhaI GCGC 2 cut(s) 92, 726
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 3 cut(s) 133, 410, 565
Hin6I GCGC 2 cut(s) 90, 724
HinP1I GCGC 2 cut(s) 90, 724
HinfI GANTC 4 cut(s) 688, 866, 881, 1024
Hpy166II GTNNAC 3 cut(s) 172, 328, 773
Hpy188I TCNGA 6 cut(s) 282, 635, 762, 865, 880, 1066
Hpy188III TCNNGA 3 cut(s) 130, 527, 794
Hpy8I GTNNAC 3 cut(s) 172, 328, 773
Hpy99I CGWCG 2 cut(s) 179, 191
HpyAV CCTTC 4 cut(s) 175, 572, 707, 812
HpyCH4III ACNGT 1 cut(s) 511
HpyCH4IV ACGT 1 cut(s) 960
HpyCH4V TGCA 8 cut(s) 260, 309, 353, 538, 788, 909, 978, 993
HpyF3I CTNAG 4 cut(s) 117, 252, 719, 885
HpySE526I ACGT 1 cut(s) 960
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 3 cut(s) 133, 410, 565
HspAI GCGC 2 cut(s) 90, 724
KroI GCCGGC 2 cut(s) 109, 216
KroNI GCCGGC 2 cut(s) 111, 218
Kzo9I GATC 1 cut(s) 126
LguI GCTCTTC 3 cut(s) 301, 323, 345
LmnI GCTCC 1 cut(s) 972
Lsp1109I GCAGC 7 cut(s) 43, 81, 90, 694, 738, 829, 962
LweI GCATC 3 cut(s) 81, 373, 829
MaeI CTAG 2 cut(s) 1046, 1072
MaeII ACGT 1 cut(s) 960
MaeIII GTNAC 1 cut(s) 956
MalI GATC 1 cut(s) 128
MbiI CCGCTC 1 cut(s) 48
MboI GATC 1 cut(s) 126
MboII GAAGA 8 cut(s) 245, 288, 310, 332, 384, 387, 465, 861
MfeI CAATTG 1 cut(s) 539
MluCI AATT 6 cut(s) 360, 530, 539, 666, 730, 823
MlyI GAGTC 2 cut(s) 890, 1018
MmeI TCCRAC 1 cut(s) 305
MreI CGCCGGCG 1 cut(s) 109
MroNI GCCGGC 2 cut(s) 109, 216
MseI TTAA 2 cut(s) 458, 809
MspA1I CMGCKG 1 cut(s) 894
MspR9I CCNGG 5 cut(s) 21, 60, 86, 87, 287
MunI CAATTG 1 cut(s) 539
Mva1269I GAATGC 1 cut(s) 1013
MvaI CCWGG 1 cut(s) 21
MvnI CGCG 2 cut(s) 92, 186
NaeI GCCGGC 2 cut(s) 111, 218
NciI CCSGG 4 cut(s) 60, 86, 87, 287
NdeII GATC 1 cut(s) 126
NgoMIV GCCGGC 2 cut(s) 109, 216
NlaIII CATG 3 cut(s) 133, 410, 565
NlaIV GGNNCC 5 cut(s) 18, 63, 104, 211, 898
NmuCI GTSAC 1 cut(s) 956
PaeR7I CTCGAG 1 cut(s) 525
PagI TCATGA 1 cut(s) 129
PceI AGGCCT 1 cut(s) 582
PciSI GCTCTTC 3 cut(s) 301, 323, 345
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 183
PctI GAATGC 1 cut(s) 1013
PdiI GCCGGC 2 cut(s) 111, 218
PfeI GAWTC 2 cut(s) 688, 866
PflFI GACNNNGTC 1 cut(s) 191
PflMI CCANNNNNTGG 1 cut(s) 205
PleI GAGTC 2 cut(s) 889, 1018
PpsI GAGTC 2 cut(s) 889, 1018
Ppu21I YACGTR 1 cut(s) 961
PpuMI RGGWCCY 1 cut(s) 263
Psp5II RGGWCCY 1 cut(s) 263
Psp6I CCWGG 1 cut(s) 19
PspGI CCWGG 1 cut(s) 19
PspN4I GGNNCC 5 cut(s) 18, 63, 104, 211, 898
PspPI GGNCC 7 cut(s) 41, 62, 210, 263, 431, 654, 897
PspPPI RGGWCCY 1 cut(s) 263
PstI CTGCAG 1 cut(s) 790
PsyI GACNNNGTC 1 cut(s) 191
RsaI GTAC 4 cut(s) 158, 329, 772, 1058
RsaNI GTAC 4 cut(s) 157, 328, 771, 1057
SapI GCTCTTC 3 cut(s) 301, 323, 345
SaqAI TTAA 2 cut(s) 458, 809
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 7 cut(s) 41, 62, 210, 263, 431, 654, 897
SchI GAGTC 2 cut(s) 890, 1018
ScrFI CCNGG 5 cut(s) 21, 60, 86, 87, 287
SfaNI GCATC 3 cut(s) 81, 373, 829
SfcI CTRYAG 2 cut(s) 519, 786
Sfr274I CTCGAG 1 cut(s) 525
SgrAI CRCCGGYG 1 cut(s) 109
SinI GGWCC 4 cut(s) 62, 210, 263, 897
SlaI CTCGAG 1 cut(s) 525
SmaI CCCGGG 1 cut(s) 87
SmlI CTYRAG 1 cut(s) 525
SmoI CTYRAG 1 cut(s) 525
Sse9I AATT 6 cut(s) 360, 530, 539, 666, 730, 823
SseBI AGGCCT 1 cut(s) 582
SspMI CTAG 2 cut(s) 1046, 1072
StuI AGGCCT 1 cut(s) 582
StyD4I CCNGG 5 cut(s) 19, 58, 84, 85, 285
StyI CCWWGG 1 cut(s) 846
TaaI ACNGT 1 cut(s) 511
TaiI ACGT 1 cut(s) 963
TaqI TCGA 3 cut(s) 526, 793, 1040
TasI AATT 6 cut(s) 360, 530, 539, 666, 730, 823
TauI GCSGC 4 cut(s) 95, 116, 140, 186
TfiI GAWTC 2 cut(s) 688, 866
Tru1I TTAA 2 cut(s) 458, 809
Tru9I TTAA 2 cut(s) 458, 809
TseFI GTSAC 1 cut(s) 956
TseI GCWGC 7 cut(s) 31, 69, 78, 707, 726, 842, 975
Tsp45I GTSAC 1 cut(s) 956
TspDTI ATGAA 4 cut(s) 360, 395, 619, 1011
TspMI CCCGGG 1 cut(s) 85
Tth111I GACNNNGTC 1 cut(s) 191
Van91I CCANNNNNTGG 1 cut(s) 205
VpaK11BI GGWCC 4 cut(s) 62, 210, 263, 897
XapI RAATTY 2 cut(s) 530, 666
XhoI CTCGAG 1 cut(s) 525
XmaI CCCGGG 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 2 cut(s) 1046, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.