Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0008061

Physical Location & Seq

Genomic Coordinates Reverse (-)

5169346 .. 5180016

Gene Structure 10671 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2631 bp

ATGCTTGGAATATGGTATGCAGTGCTTCCGGAAGAGAAAAGGGCTTCATTCTGGAAATTGGTTTTGAAGCAGTTTGATGATTTGCTTGTAAAGATCTTGATTGTTGCGGCGCTTGTTTCTTTTGTTCTGGCTTTGATTAATGGAGATACGGGATTAACAGCTTTTTTGGAGCCTTCTGTTATTCTCTTGATATTGGCTGCAAATGCTGCAGTAGGAGTGATTACAGAAACAAACGCAGAAAAGGCTCTTGAGGAGCTACGTGCATACCAAGCCGATATCGCAACTGTGCTGCGAAATGGTTGCTTCTCCATACTTCCAGCAACAGAGCTTGTCCCAGGTGATATTGTAGAAGTGGCTGTGGGAGGCAAAATTCCAGCTGATATGAGAATGATTGAGATGCTAAGTAATCAATTACGTGTTGATCAGGCAATTCTTACAGGTGAGAGTTGCTCTGTTGGAAAAGAGCTCGAGTCCACTACTGCAACAAATGTTGTATATCAAGACAAGACAAATATCCTTTTCTCGGGTACAGTAGTCGTGGCTGGTAGGGCTAGAGCTGTTGTAGTAGGAGTTGGAACACACACTGCTATGGGTGGTATACACGATTCAATGTTGCAAACAGAAGATGAAGTGACGCCATTGAAAAAGAAGCTGGATGAATTTGGTACATTCTTGGCCAAGGTTATTGCTGGCATATGTGTACTGGTATGGATTGTAAATATTGGTCACTTTCGTGACCCTGCTCATGGTGGGTTCTTGCGTGGTGCAATTCATTACTTCAAGATTGCAGTGGCACTTGCAGTTGCAGCAATCCCTGAAGGACTTCCAGCTGTCGTTACAACTTGTTTGGCTCTTGGGACAAAACGAATGGCTCGGTTGAATGCCATTGTAAGGTCTTTGCCATCTGTTGAGACTTTAGGCTGCACTACAGTAATCTGCAGTGACAAAACCGGAACACTGACAACCAATATGATGTCTGCCTCAAAGGTTTGTGTGCTTCATACCGTACAACATGCTCCTGTTATTTCCGAATACAGTGTGAGTGGAACAACATATGCGCCGGAGGGCTTAATTTTTGACAGTACTGGGCTTCAGCTTGAACTTCCAGCTCAGTCTCCCTGTCTTCTTCACATAGCAATGTGTTCAGCACTGTGCAATGAGTCCATCCTGCAATATAATCCTGACAAGAGTAACTATGAAAAAATTGGCGAGTCAACTGAAGTTGCCCTCCGTGTTCTTGCAGAGAAGATTGGTCTTCCTGGCTTTGATTCTATGCCTTCTTCTCTGAATATGCTGAGCAAGCATGAACGTGCATCTTACTGTAATCGCTACTGGGAGGACCATTTTAAAAAGATTTCTGTTGCAGATTTTTCTCGTGATCGGAAGATGATGAGTGTTCTTTGCAGCCGAAACCAATTGCAAATTATGTTTTCAAAAGGTGCTCCAGAGAGTATTATTTCTAGATGCACCAGTATTCTGTGCAATGATGATGGTTCTGCCATACCACTAACCACTAGTATCCGGGCTGAGCTGGAATCAAGGTTCCATAGTTTTGCAGGAAAGGAAACACTAAGATGCTTGGCCCTGGCTTTCAAGCGGATGCCCATGGGTCTACAGAGTCTCTCCCATAATGATGAGAAGGATCTAACATTCATTGGGTTGATCGGAATGCTTGATCCACCAAGAGAAGAAGTGAGAAATGCTATGCTTTCATGCATGACTGCTGGCATACGAGTTATAGTTGTAACTGGGGACAACAAGTCAACAGCTGAATCACTTTGCCGCAAGATAGGGGCTTTTGATCACTTAGAAGATCTTGCTGGACACTCTTACACTGCAACTGAGTTTGAAGAATTACCAGCAATGCAGAAAACACTGGCATTGCAACGCATGGCACTATTCACCAGAGTTGAACCTTCTCATAAAAGGATGCTGGTTGAGGCCTTGCGAAACCAGAACGAAGTGGTTGCAATGACTGGTGATGGTGTTAATGATGCACCTGCACTAAAAAAGGCAGATATTGGAATTGCCATGGGTTCAGGAACAGCCGTTGCCAAGAGTGCTTCAGATATGGTTTTGGCAGATGACAATTTTGCTACTATTGTTGCAGCTGTTGCAGAGGGAAGGGCTATATACAATAACACGAAGCAATTTATCAGATATATGATCTCTTCAAATATTGGTGAAGTAGTTTGTATATTTGTGGCAGCTGTACTTGGCATACCTGATACCCTAGCCCCTGTCCAGTTGCTCTGGGTCAACTTAGTTACTGATGGATTGCCTGCCACTGCTATTGGTTTTAATAAGCAAGATTCTGATGTGATGAAAGCTAAACCTCGAAAGGTCAATGAAGCAGTTGTTTCTGGATGGCTGTTTTTTCGTTATTTGGTAATTGGAGCTTATGTTGGGCTTGCCACGGTTGCGGGATTTATATGGTGGTTTGTTTACTCTGATAGCGGTCCTCAACTGCCATATTCTGAATTGATGAATTTTGACTCATGCTCAACGAGGGAGACAACTTATCCATGCAGTATATTTGATCGATCGCCATCCATCAACTGTTACACCGTTATCTTGGGCCGACTGGATTGTTGTGCTATACCTTTCATTTCCCGTTGTTATCATCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0003824 GO:0005215 GO:0005384 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006828 GO:0007275 GO:0008150 GO:0008324 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015410 GO:0015662 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022622 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0034220 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0048364 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055071 GO:0055076 GO:0055080 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071421 GO:0072511 GO:0090662 GO:0097708 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0099131 GO:0099132 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko04020 ko04022 ko04972 ko05010 map04020 map04022 map04972 map05010

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

877

Amino Acids

95.13

Weight (kDa)

5.96

Isoelectric Point (pI)

38.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	9 - 40	3.7e-07	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	90 - 203	1.3e-22	P-type ATPase actuator domain
Cation_ATPase	PF13246	377 - 564	1.7e-40	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	549 - 673	6.9e-14	haloacid dehalogenase-like hydrolase
Hydrolase_3	PF08282	645 - 700	7.1e-06	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	744 - 820	5.6e-21	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008061)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10130
fragaria_vesca	FvH4_2g32870 FvH4_2g32870 FvH4_2g32870
malus_domestica	MD08G1080200.v1.1 MD15G1067300.v1.1
prunus_persica	Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1 Prupe.1G421100_v2.0.a1
pyrus_communis	pycom08g06620 pycom15g06380
rosa_chinensis	RchiOBHm_Chr6g0309841
rosa_laevigata	RLG00000010530
rosa_multiflora	Rmu_ssc0000373.1_g000002
rosa_roxburghii	Rroxscaffold_7G00159130
rosa_rugosa	Rorug06G0375200 Rorug06G0375300
rosa_samantha	Rh6AG488700 Rh6BG498200 Rh6CG503000 Rh6DG489200
rosa_wichuraiana	Rw6G042560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2008
Acc36I	ACCTGC	1 cut(s)	2008
AccI	GTMKAC	2 cut(s)	598, 1612
AccIII	TCCGGA	1 cut(s)	28
AciI	CCGC	5 cut(s)	107, 1597, 1783, 2423, 2457
AclWI	GGATC	2 cut(s)	1650, 1670
AcoI	YGGCCR	1 cut(s)	675
AcsI	RAATTY	3 cut(s)	369, 659, 2488
AcuI	CTGAAG	4 cut(s)	837, 1076, 1239, 2049
AcyI	GRCGYC	1 cut(s)	635
AfaI	GTAC	6 cut(s)	529, 667, 702, 1008, 1084, 2214
AfiI	CCNNNNNNNGG	3 cut(s)	523, 746, 891
AflIII	ACRYGT	1 cut(s)	415
AhdI	GACNNNNNGTC	1 cut(s)	1759
AhlI	ACTAGT	1 cut(s)	1514
AjnI	CCWGG	3 cut(s)	334, 1258, 1584
AleI	CACNNNNGTG	1 cut(s)	732
AloI	GAACNNNNNNTCC	2 cut(s)	1526, 1558
Alw21I	GWGCWC	2 cut(s)	468, 1444
Alw26I	GTCTC	4 cut(s)	905, 1119, 1625, 2507
AlwI	GGATC	2 cut(s)	1650, 1670
Ama87I	CYCGRG	2 cut(s)	467, 523
Aor13HI	TCCGGA	1 cut(s)	28
AoxI	GGCC	4 cut(s)	675, 1581, 1941, 2578
ApeKI	GCWGC	8 cut(s)	197, 206, 289, 806, 921, 1404, 2108, 2207
ApoI	RAATTY	3 cut(s)	369, 659, 2488
AseI	ATTAAT	1 cut(s)	138
Asp700I	GAANNNNTTC	1 cut(s)	822
AspLEI	GCGC	2 cut(s)	112, 1060
AspS9I	GGNCC	4 cut(s)	1339, 1582, 2459, 2578
AsuC2I	CCSGG	1 cut(s)	1523
AsuHPI	GGTGA	5 cut(s)	350, 452, 1894, 1991, 2195
AvaI	CYCGRG	2 cut(s)	467, 523
AvaII	GGWCC	2 cut(s)	1339, 2459
BaeI	ACNNNNGTAYC	2 cut(s)	519, 552
BalI	TGGCCA	1 cut(s)	677
BanII	GRGCYC	1 cut(s)	468
BauI	CACGAG	1 cut(s)	1374
BbsI	GAAGAC	2 cut(s)	1115, 1247
Bbv12I	GWGCWC	2 cut(s)	468, 1444
BbvI	GCAGC	8 cut(s)	184, 193, 276, 818, 908, 1416, 2120, 2219
BccI	CCATC	8 cut(s)	910, 1172, 1484, 1976, 2267, 2361, 2557, 2561
BceAI	ACGGC	1 cut(s)	2033
BcgI	CGANNNNNNTGC	4 cut(s)	282, 316, 1949, 1983
BciT130I	CCWGG	3 cut(s)	336, 1260, 1586
BciVI	GTATCC	1 cut(s)	1529
BclI	TGATCA	2 cut(s)	421, 1801
BcnI	CCSGG	1 cut(s)	1523
BcoDI	GTCTC	4 cut(s)	905, 1119, 1625, 2507
BcuI	ACTAGT	1 cut(s)	1514
BfaI	CTAG	4 cut(s)	552, 1461, 1515, 2234
BfmI	CTRYAG	4 cut(s)	207, 927, 937, 1613
BfoI	RGCGCY	1 cut(s)	113
BfuAI	ACCTGC	1 cut(s)	2008
BfuI	GTATCC	1 cut(s)	1529
BglII	AGATCT	2 cut(s)	93, 1813
BlpI	GCTNAGC	2 cut(s)	1295, 1527
BmcAI	AGTACT	1 cut(s)	1084
Bme1390I	CCNGG	4 cut(s)	336, 1260, 1523, 1586
Bme18I	GGWCC	2 cut(s)	1339, 2459
BmeRI	GACNNNNNGTC	1 cut(s)	1759
BmeT110I	CYCGRG	2 cut(s)	467, 523
BmgT120I	GGNCC	4 cut(s)	1339, 1582, 2459, 2578
BmiI	GGNNCC	2 cut(s)	171, 1544
BmrFI	CCNGG	4 cut(s)	336, 1260, 1523, 1586
BmrI	ACTGGG	3 cut(s)	1095, 1342, 1758
BmsI	GCATC	7 cut(s)	387, 1322, 1454, 1565, 1590, 1920, 1984
BmuI	ACTGGG	3 cut(s)	1095, 1342, 1758
BpiI	GAAGAC	2 cut(s)	1115, 1247
BplI	GAGNNNNNCTC	2 cut(s)	434, 466
BpmI	CTGGAG	1 cut(s)	1428
Bpu1102I	GCTNAGC	2 cut(s)	1295, 1527
BpuEI	CTTGAG	1 cut(s)	269
BpuMI	CCSGG	1 cut(s)	1523
Bsa29I	ATCGAT	2 cut(s)	2542, 2626
BsaAI	YACGTR	2 cut(s)	260, 416
BsaBI	GATNNNNATC	1 cut(s)	2548
BsaHI	GRCGYC	1 cut(s)	635
BsaJI	CCNNGG	6 cut(s)	334, 678, 1584, 1605, 2031, 2415
BsaWI	WCCGGW	2 cut(s)	28, 950
BsaXI	ACNNNNNCTCC	2 cut(s)	2388, 2418
Bsc4I	CCNNNNNNNGG	3 cut(s)	523, 746, 891
Bse1I	ACTGG	9 cut(s)	708, 1090, 1337, 1470, 1753, 1881, 1981, 2245, 2589
Bse3DI	GCAATG	6 cut(s)	1143, 1162, 1489, 1869, 1880, 1977
Bse8I	GATNNNNATC	1 cut(s)	2548
BseAI	TCCGGA	1 cut(s)	28
BseBI	CCWGG	3 cut(s)	336, 1260, 1586
BseCI	ATCGAT	2 cut(s)	2542, 2626
BseDI	CCNNGG	6 cut(s)	334, 678, 1584, 1605, 2031, 2415
BseGI	GGATG	6 cut(s)	661, 1164, 1605, 1935, 2372, 2549
BseJI	GATNNNNATC	1 cut(s)	2548
BseLI	CCNNNNNNNGG	3 cut(s)	523, 746, 891
BseMI	GCAATG	6 cut(s)	1143, 1162, 1489, 1869, 1880, 1977
BseMII	CTCAG	4 cut(s)	1124, 1286, 1518, 1833
BseNI	ACTGG	9 cut(s)	708, 1090, 1337, 1470, 1753, 1881, 1981, 2245, 2589
BseRI	GAGGAG	1 cut(s)	266
BseXI	GCAGC	8 cut(s)	184, 193, 276, 818, 908, 1416, 2120, 2219
BsgI	GTGCAG	2 cut(s)	907, 1986
Bsh1285I	CGRYCG	1 cut(s)	2546
BshFI	GGCC	4 cut(s)	677, 1583, 1943, 2580
BshVI	ATCGAT	2 cut(s)	2542, 2626
BsiEI	CGRYCG	1 cut(s)	2546
BsiHKAI	GWGCWC	2 cut(s)	468, 1444
BsiHKCI	CYCGRG	2 cut(s)	467, 523
BsiSI	CCGG	4 cut(s)	29, 951, 1061, 1522
BslFI	GGGAC	3 cut(s)	317, 871, 1766
BslI	CCNNNNNNNGG	3 cut(s)	523, 746, 891
BsmAI	GTCTC	4 cut(s)	905, 1119, 1625, 2507
BsmFI	GGGAC	3 cut(s)	317, 871, 1766
BsmI	GAATGC	2 cut(s)	886, 1674
BsnI	GGCC	4 cut(s)	677, 1583, 1943, 2580
BsoBI	CYCGRG	2 cut(s)	467, 523
Bsp1286I	GDGCHC	2 cut(s)	468, 1444
Bsp13I	TCCGGA	1 cut(s)	28
Bsp1720I	GCTNAGC	2 cut(s)	1295, 1527
Bsp19I	CCATGG	2 cut(s)	1605, 2031
BspACI	CCGC	5 cut(s)	107, 1597, 1783, 2423, 2457
BspANI	GGCC	4 cut(s)	677, 1583, 1943, 2580
BspCNI	CTCAG	4 cut(s)	1123, 1287, 1519, 1834
BspDI	ATCGAT	2 cut(s)	2542, 2626
BspEI	TCCGGA	1 cut(s)	28
BspLI	GGNNCC	2 cut(s)	171, 1544
BspMAI	CTGCAG	2 cut(s)	211, 941
BspMI	ACCTGC	1 cut(s)	2008
BspPI	GGATC	2 cut(s)	1650, 1670
BsrDI	GCAATG	6 cut(s)	1143, 1162, 1489, 1869, 1880, 1977
BsrI	ACTGG	9 cut(s)	708, 1090, 1337, 1470, 1753, 1881, 1981, 2245, 2589
BssECI	CCNNGG	6 cut(s)	334, 678, 1584, 1605, 2031, 2415
BssNAI	GTATAC	1 cut(s)	599
BssNI	GRCGYC	1 cut(s)	635
BssSI	CACGAG	1 cut(s)	1374
BssT1I	CCWWGG	3 cut(s)	678, 1605, 2031
Bst1107I	GTATAC	1 cut(s)	599
Bst2BI	CACGAG	1 cut(s)	1374
Bst2UI	CCWGG	3 cut(s)	336, 1260, 1586
Bst6I	CTCTTC	2 cut(s)	27, 2176
BstACI	GRCGYC	1 cut(s)	635
BstAPI	GCANNNNNTGC	2 cut(s)	206, 2114
BstBAI	YACGTR	2 cut(s)	260, 416
BstC8I	GCNNGC	5 cut(s)	691, 1301, 1726, 2283, 2412
BstDEI	CTNAG	8 cut(s)	401, 1110, 1295, 1527, 1571, 1807, 1842, 2263
BstDSI	CCRYGG	3 cut(s)	1605, 2031, 2415
BstF5I	GGATG	6 cut(s)	661, 1164, 1605, 1935, 2372, 2549
BstH2I	RGCGCY	1 cut(s)	113
BstHHI	GCGC	2 cut(s)	112, 1060
BstMAI	GTCTC	4 cut(s)	905, 1119, 1625, 2507
BstMCI	CGRYCG	1 cut(s)	2546
BstNI	CCWGG	3 cut(s)	336, 1260, 1586
BstNSI	RCATGY	1 cut(s)	1016
BstSCI	CCNGG	4 cut(s)	334, 1258, 1521, 1584
BstSFI	CTRYAG	4 cut(s)	207, 927, 937, 1613
BstV1I	GCAGC	8 cut(s)	184, 193, 276, 818, 908, 1416, 2120, 2219
BstV2I	GAAGAC	2 cut(s)	1115, 1247
BstX2I	RGATCY	3 cut(s)	93, 1642, 1813
BstYI	RGATCY	3 cut(s)	93, 1642, 1813
BstZ17I	GTATAC	1 cut(s)	599
Bsu15I	ATCGAT	2 cut(s)	2542, 2626
BsuI	GTATCC	1 cut(s)	1529
BsuRI	GGCC	4 cut(s)	677, 1583, 1943, 2580
BsuTUI	ATCGAT	2 cut(s)	2542, 2626
BtgI	CCRYGG	3 cut(s)	1605, 2031, 2415
BtsCI	GGATG	6 cut(s)	661, 1164, 1605, 1935, 2372, 2549
BtsI	GCAGTG	6 cut(s)	27, 582, 795, 946, 1833, 2286
BveI	ACCTGC	1 cut(s)	2008
Cac8I	GCNNGC	5 cut(s)	691, 1301, 1726, 2283, 2412
CfoI	GCGC	2 cut(s)	112, 1060
Cfr13I	GGNCC	4 cut(s)	1339, 1582, 2459, 2578
ClaI	ATCGAT	2 cut(s)	2542, 2626
CseI	GACGC	1 cut(s)	643
Csp6I	GTAC	6 cut(s)	528, 666, 701, 1007, 1083, 2213
CspCI	CAANNNNNGTGG	2 cut(s)	2275, 2310
CviQI	GTAC	6 cut(s)	528, 666, 701, 1007, 1083, 2213
DdeI	CTNAG	8 cut(s)	401, 1110, 1295, 1527, 1571, 1807, 1842, 2263
DraI	TTTAAA	1 cut(s)	1348
DriI	GACNNNNNGTC	1 cut(s)	1759
EaeI	YGGCCR	1 cut(s)	675
Eam1104I	CTCTTC	2 cut(s)	27, 2176
Eam1105I	GACNNNNNGTC	1 cut(s)	1759
EarI	CTCTTC	2 cut(s)	27, 2176
Ecl136II	GAGCTC	1 cut(s)	466
Eco130I	CCWWGG	3 cut(s)	678, 1605, 2031
Eco147I	AGGCCT	1 cut(s)	1943
Eco24I	GRGCYC	1 cut(s)	468
Eco32I	GATATC	1 cut(s)	277
Eco47I	GGWCC	2 cut(s)	1339, 2459
Eco53kI	GAGCTC	1 cut(s)	466
Eco57I	CTGAAG	4 cut(s)	837, 1076, 1239, 2049
Eco88I	CYCGRG	2 cut(s)	467, 523
EcoICRI	GAGCTC	1 cut(s)	466
EcoRII	CCWGG	3 cut(s)	334, 1258, 1584
EcoRV	GATATC	1 cut(s)	277
EcoT14I	CCWWGG	3 cut(s)	678, 1605, 2031
EcoT22I	ATGCAT	1 cut(s)	1718
EcoT38I	GRGCYC	1 cut(s)	468
ErhI	CCWWGG	3 cut(s)	678, 1605, 2031
FaqI	GGGAC	3 cut(s)	317, 871, 1766
FauI	CCCGC	1 cut(s)	2416
FauNDI	CATATG	2 cut(s)	695, 1054
FbaI	TGATCA	2 cut(s)	421, 1801
FblI	GTMKAC	2 cut(s)	598, 1612
FokI	GGATG	6 cut(s)	668, 1151, 1612, 1942, 2379, 2536
FriOI	GRGCYC	1 cut(s)	468
FspBI	CTAG	4 cut(s)	552, 1461, 1515, 2234
GlaI	GCGC	2 cut(s)	111, 1059
GsuI	CTGGAG	1 cut(s)	1428
HaeII	RGCGCY	1 cut(s)	113
HaeIII	GGCC	4 cut(s)	677, 1583, 1943, 2580
HapII	CCGG	4 cut(s)	29, 951, 1061, 1522
HgaI	GACGC	1 cut(s)	643
HhaI	GCGC	2 cut(s)	112, 1060
Hin1I	GRCGYC	1 cut(s)	635
Hin6I	GCGC	2 cut(s)	110, 1058
HinP1I	GCGC	2 cut(s)	110, 1058
HincII	GTYRAC	3 cut(s)	1215, 1764, 2260
HindII	GTYRAC	3 cut(s)	1215, 1764, 2260
HpaII	CCGG	4 cut(s)	29, 951, 1061, 1522
HphI	GGTGA	5 cut(s)	350, 452, 1894, 1991, 2195
Hpy166II	GTNNAC	8 cut(s)	474, 599, 701, 1215, 1613, 1764, 2260, 2446
Hpy188I	TCNGA	9 cut(s)	1030, 1287, 1383, 1667, 2068, 2159, 2317, 2452, 2479
Hpy8I	GTNNAC	8 cut(s)	474, 599, 701, 1215, 1613, 1764, 2260, 2446
HpyAV	CCTTC	6 cut(s)	183, 812, 1287, 1633, 1926, 2118
HpyCH4IV	ACGT	3 cut(s)	259, 415, 1309
HpyF3I	CTNAG	8 cut(s)	401, 1110, 1295, 1527, 1571, 1807, 1842, 2263
HpySE526I	ACGT	3 cut(s)	259, 415, 1309
Hsp92I	GRCGYC	1 cut(s)	635
HspAI	GCGC	2 cut(s)	110, 1058
Kpn2I	TCCGGA	1 cut(s)	28
Ksp22I	TGATCA	2 cut(s)	421, 1801
LmnI	GCTCC	5 cut(s)	169, 253, 1021, 1447, 2396
Lsp1109I	GCAGC	8 cut(s)	184, 193, 276, 818, 908, 1416, 2120, 2219
LweI	GCATC	7 cut(s)	387, 1322, 1454, 1565, 1590, 1920, 1984
MaeI	CTAG	4 cut(s)	552, 1461, 1515, 2234
MaeII	ACGT	3 cut(s)	259, 415, 1309
MaeIII	GTNAC	9 cut(s)	631, 725, 734, 835, 941, 1190, 1744, 2266, 2561
MfeI	CAATTG	1 cut(s)	1415
MflI	RGATCY	3 cut(s)	93, 1642, 1813
MhlI	GDGCHC	2 cut(s)	468, 1444
MlsI	TGGCCA	1 cut(s)	677
MluNI	TGGCCA	1 cut(s)	677
MlyI	GAGTC	5 cut(s)	479, 1169, 1220, 1627, 2489
MmeI	TCCRAC	2 cut(s)	436, 553
Mox20I	TGGCCA	1 cut(s)	677
Mph1103I	ATGCAT	1 cut(s)	1718
MroI	TCCGGA	1 cut(s)	28
MroXI	GAANNNNTTC	1 cut(s)	822
MscI	TGGCCA	1 cut(s)	677
MseI	TTAA	6 cut(s)	138, 155, 1070, 1347, 1989, 2301
MslI	CAYNNNNRTG	6 cut(s)	587, 732, 1136, 1308, 1573, 1632
Msp20I	TGGCCA	1 cut(s)	677
MspA1I	CMGCKG	5 cut(s)	377, 830, 1769, 2111, 2210
MspI	CCGG	4 cut(s)	29, 951, 1061, 1522
MspR9I	CCNGG	4 cut(s)	336, 1260, 1523, 1586
MunI	CAATTG	1 cut(s)	1415
Mva1269I	GAATGC	2 cut(s)	886, 1674
MvaI	CCWGG	3 cut(s)	336, 1260, 1586
NciI	CCSGG	1 cut(s)	1523
NcoI	CCATGG	2 cut(s)	1605, 2031
NdeI	CATATG	2 cut(s)	695, 1054
NlaIV	GGNNCC	2 cut(s)	171, 1544
NmuCI	GTSAC	4 cut(s)	631, 725, 734, 941
NsiI	ATGCAT	1 cut(s)	1718
NspI	RCATGY	1 cut(s)	1016
OliI	CACNNNNGTG	1 cut(s)	732
PaeR7I	CTCGAG	1 cut(s)	467
PaqCI	CACCTGC	1 cut(s)	2008
PceI	AGGCCT	1 cut(s)	1943
PctI	GAATGC	2 cut(s)	886, 1674
PdmI	GAANNNNTTC	1 cut(s)	822
PfeI	GAWTC	5 cut(s)	605, 1268, 1535, 1772, 2312
Ple19I	CGATCG	1 cut(s)	2546
PleI	GAGTC	5 cut(s)	478, 1168, 1219, 1626, 2489
PpsI	GAGTC	5 cut(s)	478, 1168, 1219, 1626, 2489
Ppu21I	YACGTR	2 cut(s)	260, 416
PshBI	ATTAAT	1 cut(s)	138
Psp124BI	GAGCTC	1 cut(s)	468
Psp6I	CCWGG	3 cut(s)	334, 1258, 1584
PspGI	CCWGG	3 cut(s)	334, 1258, 1584
PspN4I	GGNNCC	2 cut(s)	171, 1544
PspPI	GGNCC	4 cut(s)	1339, 1582, 2459, 2578
PspXI	VCTCGAGB	1 cut(s)	467
PstI	CTGCAG	2 cut(s)	211, 941
PsuI	RGATCY	3 cut(s)	93, 1642, 1813
PvuI	CGATCG	1 cut(s)	2546
PvuII	CAGCTG	5 cut(s)	377, 830, 1769, 2111, 2210
RsaI	GTAC	6 cut(s)	529, 667, 702, 1008, 1084, 2214
RsaNI	GTAC	6 cut(s)	528, 666, 701, 1007, 1083, 2213
RseI	CAYNNNNRTG	6 cut(s)	587, 732, 1136, 1308, 1573, 1632
SacI	GAGCTC	1 cut(s)	468
SaqAI	TTAA	6 cut(s)	138, 155, 1070, 1347, 1989, 2301
Sau96I	GGNCC	4 cut(s)	1339, 1582, 2459, 2578
ScaI	AGTACT	1 cut(s)	1084
SchI	GAGTC	5 cut(s)	479, 1169, 1220, 1627, 2489
ScrFI	CCNGG	4 cut(s)	336, 1260, 1523, 1586
SduI	GDGCHC	2 cut(s)	468, 1444
SfaNI	GCATC	7 cut(s)	387, 1322, 1454, 1565, 1590, 1920, 1984
SfcI	CTRYAG	4 cut(s)	207, 927, 937, 1613
Sfr274I	CTCGAG	1 cut(s)	467
SinI	GGWCC	2 cut(s)	1339, 2459
SlaI	CTCGAG	1 cut(s)	467
SmiMI	CAYNNNNRTG	6 cut(s)	587, 732, 1136, 1308, 1573, 1632
SmlI	CTYRAG	2 cut(s)	248, 467
SmoI	CTYRAG	2 cut(s)	248, 467
SpeI	ACTAGT	1 cut(s)	1514
SseBI	AGGCCT	1 cut(s)	1943
SsiI	CCGC	5 cut(s)	107, 1597, 1783, 2423, 2457
SspI	AATATT	2 cut(s)	721, 2179
SspMI	CTAG	4 cut(s)	552, 1461, 1515, 2234
SstI	GAGCTC	1 cut(s)	468
StuI	AGGCCT	1 cut(s)	1943
StyD4I	CCNGG	4 cut(s)	334, 1258, 1521, 1584
StyI	CCWWGG	3 cut(s)	678, 1605, 2031
TaiI	ACGT	3 cut(s)	262, 418, 1312
TaqI	TCGA	4 cut(s)	468, 2338, 2542, 2626
TatI	WGTACW	3 cut(s)	700, 1082, 2212
TauI	GCSGC	2 cut(s)	110, 1785
TfiI	GAWTC	5 cut(s)	605, 1268, 1535, 1772, 2312
Tru1I	TTAA	6 cut(s)	138, 155, 1070, 1347, 1989, 2301
Tru9I	TTAA	6 cut(s)	138, 155, 1070, 1347, 1989, 2301
TseFI	GTSAC	4 cut(s)	631, 725, 734, 941
TseI	GCWGC	8 cut(s)	197, 206, 289, 806, 921, 1404, 2108, 2207
Tsp45I	GTSAC	4 cut(s)	631, 725, 734, 941
TspGWI	ACGGA	1 cut(s)	1220
VpaK11BI	GGWCC	2 cut(s)	1339, 2459
VspI	ATTAAT	1 cut(s)	138
XapI	RAATTY	3 cut(s)	369, 659, 2488
XbaI	TCTAGA	1 cut(s)	1460
XceI	RCATGY	1 cut(s)	1016
XhoI	CTCGAG	1 cut(s)	467
XmiI	GTMKAC	2 cut(s)	598, 1612
XmnI	GAANNNNTTC	1 cut(s)	822
XspI	CTAG	4 cut(s)	552, 1461, 1515, 2234
ZrmI	AGTACT	1 cut(s)	1084
Zsp2I	ATGCAT	1 cut(s)	1718

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.