pycom08g07280

Pumilio homolog 12-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009606
Physical Location & Seq
Reverse (-)
5864114 .. 5866520
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1380 bp
ATGGAGGGTTTTCGCGGTTTCCCGGAAACGGCGTCGCTGTGGGCTCCGGAATTTTCGTTGCCGGAATGTGTGCCCGGAAGCTCATCGTCGCAGAACCACATTGGGGGAGAATCCCATTCTGATCAAACCCTGGAGGCTGCCTTTTCTCGCCTAAATATGTCAGCTTTCAGCCGCCAGCAGTCGCGGATTTCCGGCATTGACGGCGGCGATTACAGTAAGGGGCCGGCGCTGAACTACCCATCAAGCGATCACGGATTTAGCGGCGGAAATTCGATGCCTTTTTGCGGCGGCGAGTCGTTGCAGAGTCGTCTGAACTCACAGAACGGCGGGGGTGGGGATTTAGGGAATTTTGGTCTTGAAAATTGCTGGGTGGGAAATGGCTCGAATGGCTTTGTTCCCAATCAAGATTGCTGGGTGCATTCTGACATTGCTGGGTTTCAAAAACCTCTGGATCGCCTGTCGTTGGCTGATTTGCGCGGAAATATCTCTGCTTTGGCCAAGCAGCAGCACGGCTGTCGTTTTCTGCAGAAAACAATGGAGGATGCACCGAACAGGGATGTGATTGATTTAATATTCTTGGAAATTATGGAAAATGTGGTCGTTTTGATGCTCGACCCTTACGGGAACTATGTCGTTCAGAAGCTCGTGGAGGTCTGCACCGAAGAGCAGAGGACCCGTATGCTTTCGAAGTTGATATTGGAGAGTCATACGTCACTGGTTTGTATTGCCCTCGACACTCGTGGGACTCGTACTGTACAGAAATTGTTGCAGTACGTTACTAACCAAGAGCAAGTGTCGTTGATTATGGGAGCTCTGAGCCCTGCTGCCGCTGTGTTGAGTAAGAACAACAATGGCCAACATGTGATCGAACAGTGCTTAGAGAATTTTTCTGAGGAAGACAACAGGGGTCTTCTGCTTGTGGTGGCTATTAATTGTTCCACAATTGGGAAGGACAAAAGTGGCTGTTGTGTGCTACAAAAGTGCATTGAACACTCTAGTGGTGAAAATAGAGAACGCCTTGTTGCTGCTATCATAGCGCAAGCGACAGTACTTGCAGTAGATCGCTACGGAAACTACGTGGTGCAACATTTACTGGGTTTAAGGATCCCGCAAATCACACAAAATCTTTTGAGACAACTTCAAGGCAGTTATATTTCTATATCATTGAATAAGTTTGGTAGTTGCGTTGTGGAGAAATGCTTGTCAGAGTCAAGCGAAGAACAATCTTCACAGATTATATTTGAGCTGGTCACCAACCCAAATGTGTCAATGCTTCTTGTTCACCAGTATGGGAACTTTGTTATCCAGACAGCACTCGAAGTTTCTAAGGTTAGATCATCTAAGTGCTTGGATGTTTTTACTTTACTTCATTTTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PUF

Protein Analysis

460

Amino Acids

50.22

Weight (kDa)

5.43

Isoelectric Point (pI)

43.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUF PF00806 161 - 196 1.1e-06 Pumilio-family RNA binding repeat
PUF PF00806 198 - 232 2e-11 Pumilio-family RNA binding repeat
PUF PF00806 311 - 345 9.5e-12 Pumilio-family RNA binding repeat
PUF PF00806 385 - 416 1.6e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 15, 184, 477
AccIII TCCGGA 1 cut(s) 46
AclWI GGATC 3 cut(s) 459, 1099, 1112
AcoI YGGCCR 2 cut(s) 495, 853
AcsI RAATTY 4 cut(s) 50, 268, 346, 883
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 4 cut(s) 751, 756, 773, 1050
AfiI CCNNNNNNNGG 5 cut(s) 28, 103, 284, 463, 945
AflIII ACRYGT 1 cut(s) 859
AgsI TTSAA 5 cut(s) 359, 440, 989, 1142, 1168
AjnI CCWGG 1 cut(s) 129
AjuI GAANNNNNNNTTGG 2 cut(s) 680, 712
AleI CACNNNNGTG 1 cut(s) 996
AluBI AGCT 5 cut(s) 81, 164, 643, 812, 1246
AluI AGCT 5 cut(s) 81, 164, 643, 812, 1246
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 1 cut(s) 1126
AlwI GGATC 3 cut(s) 459, 1099, 1112
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 3 cut(s) 221, 495, 853
ApeKI GCWGC 5 cut(s) 137, 502, 505, 824, 1025
ApoI RAATTY 4 cut(s) 50, 268, 346, 883
AseI ATTAAT 1 cut(s) 930
AspLEI GCGC 3 cut(s) 229, 477, 1039
AspS9I GGNCC 2 cut(s) 221, 672
AsuC2I CCSGG 2 cut(s) 23, 75
AsuHPI GGTGA 3 cut(s) 1013, 1243, 1274
AsuII TTCGAA 1 cut(s) 686
AvaII GGWCC 1 cut(s) 672
BaeGI GKGCMC 1 cut(s) 75
BalI TGGCCA 2 cut(s) 497, 855
BamHI GGATCC 1 cut(s) 1104
BanII GRGCYC 3 cut(s) 46, 814, 821
BauI CACGAG 2 cut(s) 644, 738
BbsI GAAGAC 2 cut(s) 902, 903
Bbv12I GWGCWC 1 cut(s) 814
BbvI GCAGC 5 cut(s) 124, 514, 517, 811, 1012
BccI CCATC 1 cut(s) 247
BceAI ACGGC 4 cut(s) 45, 217, 340, 526
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BciT130I CCWGG 1 cut(s) 131
BclI TGATCA 1 cut(s) 121
BcnI CCSGG 2 cut(s) 23, 75
BcoDI GTCTC 1 cut(s) 1126
BfaI CTAG 1 cut(s) 996
BfmI CTRYAG 1 cut(s) 524
BfoI RGCGCY 1 cut(s) 230
BmcAI AGTACT 1 cut(s) 1050
Bme1390I CCNGG 3 cut(s) 23, 75, 131
Bme18I GGWCC 1 cut(s) 672
BmgT120I GGNCC 2 cut(s) 221, 672
BmiI GGNNCC 4 cut(s) 45, 222, 674, 1106
BmrFI CCNGG 3 cut(s) 23, 75, 131
BmrI ACTGGG 1 cut(s) 1103
BmsI GCATC 3 cut(s) 264, 532, 597
BmuI ACTGGG 1 cut(s) 1103
BpiI GAAGAC 2 cut(s) 902, 903
BpmI CTGGAG 1 cut(s) 152
Bpu14I TTCGAA 1 cut(s) 686
BpuMI CCSGG 2 cut(s) 23, 75
BsaAI YACGTR 1 cut(s) 1078
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 129
BsaWI WCCGGW 1 cut(s) 46
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 103, 284, 463, 945
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 3 cut(s) 720, 1098, 1285
Bse3DI GCAATG 1 cut(s) 426
BseAI TCCGGA 1 cut(s) 46
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 3 cut(s) 547, 562, 1357
BseLI CCNNNNNNNGG 5 cut(s) 28, 103, 284, 463, 945
BseMI GCAATG 1 cut(s) 426
BseMII CTCAG 2 cut(s) 806, 882
BseNI ACTGG 3 cut(s) 720, 1098, 1285
BseSI GKGCMC 1 cut(s) 75
BseXI GCAGC 5 cut(s) 124, 514, 517, 811, 1012
BseYI CCCAGC 3 cut(s) 366, 411, 431
BsgI GTGCAG 1 cut(s) 640
Bsh1236I CGCG 3 cut(s) 15, 184, 477
BshFI GGCC 3 cut(s) 223, 497, 855
BsiHKAI GWGCWC 1 cut(s) 814
BsiSI CCGG 6 cut(s) 23, 47, 62, 75, 192, 224
BslFI GGGAC 1 cut(s) 757
BslI CCNNNNNNNGG 5 cut(s) 28, 103, 284, 463, 945
BsmAI GTCTC 1 cut(s) 1126
BsmFI GGGAC 1 cut(s) 757
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 3 cut(s) 223, 497, 855
Bsp119I TTCGAA 1 cut(s) 686
Bsp1286I GDGCHC 4 cut(s) 46, 75, 814, 821
Bsp13I TCCGGA 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 754
Bsp143I GATC 7 cut(s) 121, 247, 451, 864, 1060, 1104, 1334
BspANI GGCC 3 cut(s) 223, 497, 855
BspCNI CTCAG 2 cut(s) 807, 883
BspEI TCCGGA 1 cut(s) 46
BspFNI CGCG 3 cut(s) 15, 184, 477
BspLI GGNNCC 4 cut(s) 45, 222, 674, 1106
BspMAI CTGCAG 1 cut(s) 528
BspPI GGATC 3 cut(s) 459, 1099, 1112
BspQI GCTCTTC 1 cut(s) 657
BspT104I TTCGAA 1 cut(s) 686
BsrDI GCAATG 1 cut(s) 426
BsrFI RCCGGY 1 cut(s) 223
BsrGI TGTACA 1 cut(s) 754
BsrI ACTGG 3 cut(s) 720, 1098, 1285
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 7 cut(s) 121, 247, 451, 864, 1060, 1104, 1334
BssNI GRCGYC 1 cut(s) 32
BssSI CACGAG 2 cut(s) 644, 738
Bst2BI CACGAG 2 cut(s) 644, 738
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 4 cut(s) 215, 754, 873, 1048
Bst6I CTCTTC 1 cut(s) 657
BstACI GRCGYC 1 cut(s) 32
BstAUI TGTACA 1 cut(s) 754
BstBAI YACGTR 1 cut(s) 1078
BstBI TTCGAA 1 cut(s) 686
BstC8I GCNNGC 3 cut(s) 176, 225, 1041
BstDEI CTNAG 5 cut(s) 815, 877, 891, 1326, 1341
BstEII GGTNACC 1 cut(s) 1249
BstF5I GGATG 3 cut(s) 547, 562, 1357
BstFNI CGCG 3 cut(s) 15, 184, 477
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 3 cut(s) 229, 477, 1039
BstKTI GATC 7 cut(s) 124, 250, 454, 867, 1063, 1107, 1337
BstMAI GTCTC 1 cut(s) 1126
BstMBI GATC 7 cut(s) 121, 247, 451, 864, 1060, 1104, 1334
BstMWI GCNNNNNNNGC 3 cut(s) 201, 387, 1034
BstNI CCWGG 1 cut(s) 131
BstNSI RCATGY 1 cut(s) 863
BstPI GGTNACC 1 cut(s) 1249
BstSCI CCNGG 3 cut(s) 21, 73, 129
BstSFI CTRYAG 1 cut(s) 524
BstSLI GKGCMC 1 cut(s) 75
BstUI CGCG 3 cut(s) 15, 184, 477
BstV1I GCAGC 5 cut(s) 124, 514, 517, 811, 1012
BstV2I GAAGAC 2 cut(s) 902, 903
BstX2I RGATCY 1 cut(s) 1104
BstYI RGATCY 1 cut(s) 1104
BsuRI GGCC 3 cut(s) 223, 497, 855
BtsCI GGATG 3 cut(s) 547, 562, 1357
BtsIMutI CAGTG 2 cut(s) 713, 878
Cac8I GCNNGC 3 cut(s) 176, 225, 1041
CfoI GCGC 3 cut(s) 229, 477, 1039
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 2 cut(s) 221, 672
CseI GACGC 1 cut(s) 21
Csp6I GTAC 4 cut(s) 750, 755, 772, 1049
CviAII CATG 1 cut(s) 860
CviQI GTAC 4 cut(s) 750, 755, 772, 1049
DdeI CTNAG 5 cut(s) 815, 877, 891, 1326, 1341
DpnI GATC 7 cut(s) 123, 249, 453, 866, 1062, 1106, 1336
DpnII GATC 7 cut(s) 121, 247, 451, 864, 1060, 1104, 1334
EaeI YGGCCR 2 cut(s) 495, 853
Eam1104I CTCTTC 1 cut(s) 657
EarI CTCTTC 1 cut(s) 657
EciI GGCGGA 1 cut(s) 279
Ecl136II GAGCTC 1 cut(s) 812
Eco24I GRGCYC 3 cut(s) 46, 814, 821
Eco47I GGWCC 1 cut(s) 672
Eco53kI GAGCTC 1 cut(s) 812
Eco91I GGTNACC 1 cut(s) 1249
EcoICRI GAGCTC 1 cut(s) 812
EcoO109I RGGNCCY 1 cut(s) 672
EcoO65I GGTNACC 1 cut(s) 1249
EcoRII CCWGG 1 cut(s) 129
EcoT38I GRGCYC 3 cut(s) 46, 814, 821
FaeI CATG 1 cut(s) 863
FaqI GGGAC 1 cut(s) 757
FatI CATG 1 cut(s) 859
FauI CCCGC 2 cut(s) 320, 1116
FbaI TGATCA 1 cut(s) 121
FokI GGATG 3 cut(s) 554, 569, 1364
FriOI GRGCYC 3 cut(s) 46, 814, 821
FspBI CTAG 1 cut(s) 996
GlaI GCGC 3 cut(s) 228, 476, 1038
GsaI CCCAGC 3 cut(s) 370, 415, 435
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 3 cut(s) 223, 497, 855
HapII CCGG 6 cut(s) 23, 47, 62, 75, 192, 224
HgaI GACGC 1 cut(s) 21
HhaI GCGC 3 cut(s) 229, 477, 1039
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 1 cut(s) 863
Hin6I GCGC 3 cut(s) 227, 475, 1037
HinP1I GCGC 3 cut(s) 227, 475, 1037
HinfI GANTC 6 cut(s) 110, 293, 304, 703, 745, 1208
HpaII CCGG 6 cut(s) 23, 47, 62, 75, 192, 224
HphI GGTGA 3 cut(s) 1013, 1243, 1274
Hpy166II GTNNAC 1 cut(s) 1282
Hpy188I TCNGA 7 cut(s) 121, 312, 424, 639, 816, 892, 1207
Hpy188III TCNNGA 5 cut(s) 47, 356, 404, 449, 1306
Hpy8I GTNNAC 1 cut(s) 1282
Hpy99I CGWCG 2 cut(s) 37, 91
HpyAV CCTTC 1 cut(s) 943
HpyCH4III ACNGT 4 cut(s) 215, 754, 873, 1048
HpyCH4IV ACGT 3 cut(s) 710, 774, 1077
HpyCH4V TGCA 9 cut(s) 301, 418, 526, 545, 657, 769, 984, 1055, 1084
HpyF10VI GCNNNNNNNGC 3 cut(s) 201, 387, 1034
HpyF3I CTNAG 5 cut(s) 815, 877, 891, 1326, 1341
HpySE526I ACGT 3 cut(s) 710, 774, 1077
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 1 cut(s) 863
HspAI GCGC 3 cut(s) 227, 475, 1037
Kpn2I TCCGGA 1 cut(s) 46
KroI GCCGGC 1 cut(s) 223
KroNI GCCGGC 1 cut(s) 225
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 7 cut(s) 121, 247, 451, 864, 1060, 1104, 1334
LguI GCTCTTC 1 cut(s) 657
LmnI GCTCC 2 cut(s) 49, 809
Lsp1109I GCAGC 5 cut(s) 124, 514, 517, 811, 1012
LweI GCATC 3 cut(s) 264, 532, 597
MaeI CTAG 1 cut(s) 996
MaeII ACGT 3 cut(s) 710, 774, 1077
MaeIII GTNAC 3 cut(s) 711, 775, 1249
MalI GATC 7 cut(s) 123, 249, 453, 866, 1062, 1106, 1336
MboI GATC 7 cut(s) 121, 247, 451, 864, 1060, 1104, 1334
MboII GAAGA 5 cut(s) 674, 902, 908, 1218, 1229
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 1 cut(s) 1104
MhlI GDGCHC 4 cut(s) 46, 75, 814, 821
MlsI TGGCCA 2 cut(s) 497, 855
MluCI AATT 9 cut(s) 50, 268, 346, 361, 582, 761, 883, 931, 942
MluNI TGGCCA 2 cut(s) 497, 855
MlyI GAGTC 5 cut(s) 302, 313, 712, 739, 1217
MnlI CCTC 7 cut(s) 127, 456, 532, 643, 663, 740, 886
Mox20I TGGCCA 2 cut(s) 497, 855
MroI TCCGGA 1 cut(s) 46
MroNI GCCGGC 1 cut(s) 223
MscI TGGCCA 2 cut(s) 497, 855
MseI TTAA 3 cut(s) 569, 930, 1100
MslI CAYNNNNRTG 3 cut(s) 996, 1287, 1342
Msp20I TGGCCA 2 cut(s) 497, 855
MspA1I CMGCKG 1 cut(s) 830
MspI CCGG 6 cut(s) 23, 47, 62, 75, 192, 224
MspR9I CCNGG 3 cut(s) 23, 75, 131
MunI CAATTG 1 cut(s) 942
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 1 cut(s) 131
MvnI CGCG 3 cut(s) 15, 184, 477
MwoI GCNNNNNNNGC 3 cut(s) 201, 387, 1034
NaeI GCCGGC 1 cut(s) 225
NciI CCSGG 2 cut(s) 23, 75
NdeII GATC 7 cut(s) 121, 247, 451, 864, 1060, 1104, 1334
NgoMIV GCCGGC 1 cut(s) 223
NlaIII CATG 1 cut(s) 863
NlaIV GGNNCC 4 cut(s) 45, 222, 674, 1106
NmuCI GTSAC 2 cut(s) 711, 1249
NspI RCATGY 1 cut(s) 863
NspV TTCGAA 1 cut(s) 686
OliI CACNNNNGTG 1 cut(s) 996
PciI ACATGT 1 cut(s) 859
PciSI GCTCTTC 1 cut(s) 657
PcsI WCGNNNNNNNCGW 2 cut(s) 745, 1074
PctI GAATGC 1 cut(s) 418
PdiI GCCGGC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 110
PfoI TCCNGGA 1 cut(s) 21
PleI GAGTC 5 cut(s) 301, 312, 711, 739, 1216
PpsI GAGTC 5 cut(s) 301, 312, 711, 739, 1216
Ppu21I YACGTR 1 cut(s) 1078
PpuMI RGGWCCY 1 cut(s) 672
PscI ACATGT 1 cut(s) 859
PshBI ATTAAT 1 cut(s) 930
Psp124BI GAGCTC 1 cut(s) 814
Psp5II RGGWCCY 1 cut(s) 672
Psp6I CCWGG 1 cut(s) 129
PspEI GGTNACC 1 cut(s) 1249
PspFI CCCAGC 3 cut(s) 366, 411, 431
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 4 cut(s) 45, 222, 674, 1106
PspPI GGNCC 2 cut(s) 221, 672
PspPPI RGGWCCY 1 cut(s) 672
PstI CTGCAG 1 cut(s) 528
PsuI RGATCY 1 cut(s) 1104
RsaI GTAC 4 cut(s) 751, 756, 773, 1050
RsaNI GTAC 4 cut(s) 750, 755, 772, 1049
RseI CAYNNNNRTG 3 cut(s) 996, 1287, 1342
SacI GAGCTC 1 cut(s) 814
SapI GCTCTTC 1 cut(s) 657
SaqAI TTAA 3 cut(s) 569, 930, 1100
Sau3AI GATC 7 cut(s) 121, 247, 451, 864, 1060, 1104, 1334
Sau96I GGNCC 2 cut(s) 221, 672
ScaI AGTACT 1 cut(s) 1050
SchI GAGTC 5 cut(s) 302, 313, 712, 739, 1217
ScrFI CCNGG 3 cut(s) 23, 75, 131
SduI GDGCHC 4 cut(s) 46, 75, 814, 821
SfaNI GCATC 3 cut(s) 264, 532, 597
SfcI CTRYAG 1 cut(s) 524
SfuI TTCGAA 1 cut(s) 686
SinI GGWCC 1 cut(s) 672
SmiMI CAYNNNNRTG 3 cut(s) 996, 1287, 1342
Sse9I AATT 9 cut(s) 50, 268, 346, 361, 582, 761, 883, 931, 942
SspI AATATT 1 cut(s) 573
SspMI CTAG 1 cut(s) 996
SstI GAGCTC 1 cut(s) 814
StyD4I CCNGG 3 cut(s) 21, 73, 129
TaaI ACNGT 4 cut(s) 215, 754, 873, 1048
TaiI ACGT 3 cut(s) 713, 777, 1080
TaqI TCGA 7 cut(s) 272, 383, 612, 686, 732, 867, 1317
TasI AATT 9 cut(s) 50, 268, 346, 361, 582, 761, 883, 931, 942
TatI WGTACW 2 cut(s) 754, 1048
TauI GCSGC 6 cut(s) 174, 207, 264, 288, 291, 830
TfiI GAWTC 1 cut(s) 110
Tru1I TTAA 3 cut(s) 569, 930, 1100
Tru9I TTAA 3 cut(s) 569, 930, 1100
TscAI CASTG 2 cut(s) 720, 878
TseFI GTSAC 2 cut(s) 711, 1249
TseI GCWGC 5 cut(s) 137, 502, 505, 824, 1025
Tsp45I GTSAC 2 cut(s) 711, 1249
TspDTI ATGAA 1 cut(s) 1358
TspGWI ACGGA 2 cut(s) 267, 1083
TspRI CASTG 2 cut(s) 720, 878
VpaK11BI GGWCC 1 cut(s) 672
VspI ATTAAT 1 cut(s) 930
XapI RAATTY 4 cut(s) 50, 268, 346, 883
XceI RCATGY 1 cut(s) 863
XspI CTAG 1 cut(s) 996
ZrmI AGTACT 1 cut(s) 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.