pycom08g12130

EG45-like domain containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010530
Physical Location & Seq
Reverse (-)
10420372 .. 10433507
13136 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGTTAGTCGATCAGTTTCTAATTTGTTTCTCCTGCACTCCATCAGCCTTCAGGCCCCTATTCTTTTCGCTGATCACGCTTACCAGGCAAATGTCTAAGCCTCAGAGTTCTCTTCCATGCCTGCTTCTGATCTTCTTATCCGAAGCGCTCTTTCATTCCTCTCAGGCTGACGTTGGCACTGCTGCTCAGTACAGGCCCCCTTATTTACCTACCGCCTGTGGTGGAAGTGACCAGGGACAGTTTCCTCCAAGCAACCTGTTCGCGTCCGTTGGCGAGGGAATTTGGGACAATGGTGCAGCTTGTGGAAGGCAATATCAGGTGAGGTGCATAAGCGCTCCCGTGCGAGGAACCTGCGTTCCAGGGAACACGATTCAGGTAAAGATTGTAGATCGTGCACTCTCTTCGGTGTCCAGACCTTCAAGAAGTGATGCTACCATAGTTCTCTCTACTACTGCATTTGCTGCAATCGCCAACTCTTCCGCAACATTCGTTAACATAGAATTTCAACAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1

Protein Analysis

172

Amino Acids

18.35

Weight (kDa)

8.21

Isoelectric Point (pI)

48.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 93 - 167 1.6e-10 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 359
AccII CGCG 1 cut(s) 263
AciI CCGC 2 cut(s) 213, 480
AcsI RAATTY 2 cut(s) 279, 500
AcuI CTGAAG 1 cut(s) 34
AfaI GTAC 1 cut(s) 191
AfeI AGCGCT 2 cut(s) 147, 334
AfiI CCNNNNNNNGG 1 cut(s) 344
AgsI TTSAA 2 cut(s) 420, 506
AjnI CCWGG 3 cut(s) 83, 231, 358
AloI GAACNNNNNNTCC 4 cut(s) 339, 340, 371, 372
AluBI AGCT 1 cut(s) 299
AluI AGCT 1 cut(s) 299
Alw21I GWGCWC 1 cut(s) 397
Alw44I GTGCAC 1 cut(s) 393
Aor51HI AGCGCT 2 cut(s) 147, 334
AoxI GGCC 2 cut(s) 53, 194
ApaLI GTGCAC 1 cut(s) 393
ApeKI GCWGC 3 cut(s) 182, 296, 461
ApoI RAATTY 2 cut(s) 279, 500
AspLEI GCGC 2 cut(s) 148, 335
AspS9I GGNCC 2 cut(s) 54, 195
AsuHPI GGTGA 1 cut(s) 331
BaeGI GKGCMC 1 cut(s) 397
BarI GAAGNNNNNNTAC 2 cut(s) 415, 447
Bbv12I GWGCWC 1 cut(s) 397
BbvI GCAGC 3 cut(s) 169, 308, 448
BccI CCATC 1 cut(s) 49
BcgI CGANNNNNNTGC 6 cut(s) 241, 275, 333, 367, 384, 418
BciT130I CCWGG 3 cut(s) 85, 233, 360
BclI TGATCA 1 cut(s) 72
BfoI RGCGCY 2 cut(s) 149, 336
BfuAI ACCTGC 1 cut(s) 359
BisI GCNGC 3 cut(s) 183, 297, 462
BlsI GCNGC 3 cut(s) 184, 298, 463
Bme1390I CCNGG 3 cut(s) 85, 233, 360
BmgT120I GGNCC 2 cut(s) 54, 195
BmiI GGNNCC 3 cut(s) 56, 197, 349
BmrFI CCNGG 3 cut(s) 85, 233, 360
BmsI GCATC 1 cut(s) 418
BsaJI CCNNGG 2 cut(s) 232, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 344
BseBI CCWGG 3 cut(s) 85, 233, 360
BseDI CCNNGG 2 cut(s) 232, 359
BseLI CCNNNNNNNGG 1 cut(s) 344
BseMII CTCAG 3 cut(s) 116, 176, 200
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 3 cut(s) 169, 308, 448
BsgI GTGCAG 2 cut(s) 19, 315
Bsh1236I CGCG 1 cut(s) 263
BshFI GGCC 2 cut(s) 55, 196
BsiHKAI GWGCWC 1 cut(s) 397
BslFI GGGAC 2 cut(s) 249, 299
BslI CCNNNNNNNGG 1 cut(s) 344
BsmFI GGGAC 2 cut(s) 249, 299
BsnI GGCC 2 cut(s) 55, 196
Bsp1286I GDGCHC 1 cut(s) 397
Bsp143I GATC 4 cut(s) 10, 72, 129, 388
BspACI CCGC 2 cut(s) 213, 480
BspANI GGCC 2 cut(s) 55, 196
BspCNI CTCAG 3 cut(s) 115, 175, 199
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 3 cut(s) 56, 197, 349
BspMI ACCTGC 1 cut(s) 359
BssECI CCNNGG 2 cut(s) 232, 359
BssMI GATC 4 cut(s) 10, 72, 129, 388
Bst2UI CCWGG 3 cut(s) 85, 233, 360
Bst4CI ACNGT 1 cut(s) 240
Bst6I CTCTTC 3 cut(s) 117, 406, 481
BstAPI GCANNNNNTGC 1 cut(s) 461
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 4 cut(s) 96, 102, 162, 186
BstFNI CGCG 1 cut(s) 263
BstH2I RGCGCY 2 cut(s) 149, 336
BstHHI GCGC 2 cut(s) 148, 335
BstKTI GATC 4 cut(s) 13, 75, 132, 391
BstMBI GATC 4 cut(s) 10, 72, 129, 388
BstMWI GCNNNNNNNGC 4 cut(s) 76, 85, 461, 467
BstNI CCWGG 3 cut(s) 85, 233, 360
BstSCI CCNGG 3 cut(s) 83, 231, 358
BstSLI GKGCMC 1 cut(s) 397
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 3 cut(s) 169, 308, 448
BsuRI GGCC 2 cut(s) 55, 196
BtsI GCAGTG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 177
BveI ACCTGC 1 cut(s) 359
Cac8I GCNNGC 1 cut(s) 122
CfoI GCGC 2 cut(s) 148, 335
Cfr13I GGNCC 2 cut(s) 54, 195
CseI GACGC 1 cut(s) 252
Csp6I GTAC 1 cut(s) 190
CviAII CATG 1 cut(s) 117
CviJI RGCY 6 cut(s) 47, 55, 100, 167, 196, 299
CviKI_1 RGCY 6 cut(s) 47, 55, 100, 167, 196, 299
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 4 cut(s) 96, 102, 162, 186
DpnI GATC 4 cut(s) 12, 74, 131, 390
DpnII GATC 4 cut(s) 10, 72, 129, 388
Eam1104I CTCTTC 3 cut(s) 117, 406, 481
EarI CTCTTC 3 cut(s) 117, 406, 481
Eco47III AGCGCT 2 cut(s) 147, 334
Eco57I CTGAAG 1 cut(s) 34
EcoO109I RGGNCCY 2 cut(s) 54, 195
EcoRII CCWGG 3 cut(s) 83, 231, 358
FaeI CATG 1 cut(s) 120
FaiI YATR 5 cut(s) 118, 329, 437, 497, 514
FaqI GGGAC 2 cut(s) 249, 299
FatI CATG 1 cut(s) 116
FbaI TGATCA 1 cut(s) 72
Fnu4HI GCNGC 3 cut(s) 183, 297, 462
Fsp4HI GCNGC 3 cut(s) 183, 297, 462
GlaI GCGC 2 cut(s) 147, 334
GluI GCNGC 3 cut(s) 183, 297, 462
HaeII RGCGCY 2 cut(s) 149, 336
HaeIII GGCC 2 cut(s) 55, 196
HgaI GACGC 1 cut(s) 252
HhaI GCGC 2 cut(s) 148, 335
Hin1II CATG 1 cut(s) 120
Hin6I GCGC 2 cut(s) 146, 333
HinP1I GCGC 2 cut(s) 146, 333
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 1 cut(s) 370
HpaI GTTAAC 1 cut(s) 493
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 2 cut(s) 395, 493
Hpy188I TCNGA 3 cut(s) 105, 129, 142
Hpy188III TCNNGA 2 cut(s) 411, 420
Hpy8I GTNNAC 2 cut(s) 395, 493
HpyAV CCTTC 3 cut(s) 58, 300, 426
HpyCH4III ACNGT 1 cut(s) 240
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 6 cut(s) 36, 296, 327, 395, 455, 464
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 85, 461, 467
HpyF3I CTNAG 4 cut(s) 96, 102, 162, 186
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 1 cut(s) 120
HspAI GCGC 2 cut(s) 146, 333
Ksp22I TGATCA 1 cut(s) 72
KspAI GTTAAC 1 cut(s) 493
Kzo9I GATC 4 cut(s) 10, 72, 129, 388
LmnI GCTCC 1 cut(s) 340
Lsp1109I GCAGC 3 cut(s) 169, 308, 448
LweI GCATC 1 cut(s) 418
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 227
MalI GATC 4 cut(s) 12, 74, 131, 390
MboI GATC 4 cut(s) 10, 72, 129, 388
MboII GAAGA 4 cut(s) 104, 124, 393, 468
MhlI GDGCHC 1 cut(s) 397
MluCI AATT 3 cut(s) 21, 279, 500
MnlI CCTC 6 cut(s) 111, 169, 255, 268, 315, 338
MseI TTAA 1 cut(s) 492
MspR9I CCNGG 3 cut(s) 85, 233, 360
MvaI CCWGG 3 cut(s) 85, 233, 360
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 4 cut(s) 76, 85, 461, 467
NdeII GATC 4 cut(s) 10, 72, 129, 388
NlaIII CATG 1 cut(s) 120
NlaIV GGNNCC 3 cut(s) 56, 197, 349
NmuCI GTSAC 1 cut(s) 227
PfeI GAWTC 1 cut(s) 370
PkrI GCNGC 3 cut(s) 184, 298, 463
Psp6I CCWGG 3 cut(s) 83, 231, 358
PspGI CCWGG 3 cut(s) 83, 231, 358
PspN4I GGNNCC 3 cut(s) 56, 197, 349
PspPI GGNCC 2 cut(s) 54, 195
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
SaqAI TTAA 1 cut(s) 492
SatI GCNGC 3 cut(s) 183, 297, 462
Sau3AI GATC 4 cut(s) 10, 72, 129, 388
Sau96I GGNCC 2 cut(s) 54, 195
ScrFI CCNGG 3 cut(s) 85, 233, 360
SduI GDGCHC 1 cut(s) 397
SetI ASST 9 cut(s) 174, 211, 258, 301, 321, 326, 353, 378, 418
SfaNI GCATC 1 cut(s) 418
Sse9I AATT 3 cut(s) 21, 279, 500
SsiI CCGC 2 cut(s) 213, 480
StyD4I CCNGG 3 cut(s) 83, 231, 358
TaaI ACNGT 1 cut(s) 240
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 9
TasI AATT 3 cut(s) 21, 279, 500
TatI WGTACW 1 cut(s) 189
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 1 cut(s) 492
Tru9I TTAA 1 cut(s) 492
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 3 cut(s) 182, 296, 461
Tsp45I GTSAC 1 cut(s) 227
TspDTI ATGAA 1 cut(s) 143
TspGWI ACGGA 1 cut(s) 256
TspRI CASTG 1 cut(s) 184
VneI GTGCAC 1 cut(s) 393
XapI RAATTY 2 cut(s) 279, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.