pycom08g16590

GEM-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012089
Physical Location & Seq
Forward (+)
16556684 .. 16560566
3883 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1077 bp
ATGGACTGCAAATTCCCCTCTTTACAAACTGTCCAAATCCAAAATCCAAAATTCAAAATCCAAAATCCCAAATTAATCTCTTCGCACCAGTTGTCTTCTCGCAGACTCCGCCGCCGAACAAGCCGTTTGAATTCCCAAACACGACAAAGATTCCATTGTCCCAATCACTACAAAATGACCGACCACAAGGTGGCAGCAGCACCGCCTGCACCTGATCACCCGCCACCGCCATCTCACTCCGAACACTACGCTCCATACCCTAAGGTCGATCTCAACGACGTCGCTCCGCCTCCCCACTCCGAAACTTGGACCTCCGTCTCCATCGCCTCTGAAACGCCTCCACCCCCGAAGTCGGAGGGCCCGCCGGTGCAGCCGCCTGTTCCGCCACCGGTGGTTCAAGCCGAGGCTCGCGGTCCGATCGATCTTCCCGGCGACGCCGCCACCTCCATGCCGGCGGAGTCCAATCCCTACGTCGCCACTCCTGCGCCGCCTCCCCCCTCCTCCGTCAAGAAATCGATGGATTCAGTGAAAGATGCTCTCGGAAAATGGGGAAAGAAGGCTGCGGAGGCCACCAAGAAGGCCGAGGATCTTGCTGGCAACATGTGGCAACACTTGAAAACGGGACCCAGTTTTGCTGATGCCGCTGTGACAAGAATTGCTCAGAGCACAAAAGTACTTGCAGAAGGTGGTTATGAGAAGATCTTTCGACACAATTTTGAGACTGTCCCAGAGGAGCAACTACTAAAGACATTTGCTTGCTACCTATCCACATCAGCTGGCCCGGTAATGGGAATTTTATATTTGTCATCAGCTAAGCTTGCATTTTGCAGTGACGATCCCCTTTCATACACAGTTGGTGACCGGACTGAGTGGAGCTATTATAAGGTGATTATTCCATTACATCAGCTGAAGGCAGTCAATCCATCAACGAGCAAAGTGAAAGCAGCTGAAAAGTACATCCAGGTCGTCTCTGTTGACAACCATGAGTTTTGGTTCATGAGCTTTGTGCACTACGATAGTGCTGTGAAACATCTTCAAGAAGCGCTGCAGCCGCATCCTCATCCCCACAGCGTATGA

Protein Analysis

359

Amino Acids

39.5

Weight (kDa)

8.94

Isoelectric Point (pI)

55.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAM PF02893 234 - 350 6.8e-19 GRAM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012089)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28200 AT1G28200
fragaria_vesca FvH4_5g35910
malus_domestica MD08G1192100.v1.1 MD15G1380800.v1.1
prunus_persica Prupe.1G527000_v2.0.a1
pyrus_communis pycom08g16590 pycom15g34130
rosa_chinensis RchiOBHm_Chr7g0237641
rosa_laevigata RLG00000000979
rosa_multiflora Rmu_sc0007826.1_g000010
rosa_roxburghii Rroxscaffold_3G00223910
rosa_rugosa Rorug07G0305800 Rorug07G0305900
rosa_samantha Rh7AG462600 Rh7BG432600 Rh7CG479400 Rh7DG448400
rosa_wichuraiana Rw7G038270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
AatII GACGTC 1 cut(s) 282
AccB7I CCANNNNNTGG 1 cut(s) 190
AccII CGCG 1 cut(s) 411
AclWI GGATC 2 cut(s) 594, 830
AcsI RAATTY 4 cut(s) 11, 50, 130, 792
AcuI CTGAAG 1 cut(s) 929
AcyI GRCGYC 2 cut(s) 279, 435
AdeI CACNNNGTG 1 cut(s) 190
AfaI GTAC 2 cut(s) 675, 956
AfeI AGCGCT 1 cut(s) 1044
AfiI CCNNNNNNNGG 5 cut(s) 190, 306, 352, 787, 788
AflIII ACRYGT 1 cut(s) 600
AgeI ACCGGT 1 cut(s) 388
AgsI TTSAA 5 cut(s) 55, 130, 398, 616, 1037
AjnI CCWGG 1 cut(s) 960
AluBI AGCT 7 cut(s) 776, 812, 817, 876, 907, 947, 1002
AluI AGCT 7 cut(s) 776, 812, 817, 876, 907, 947, 1002
Alw21I GWGCWC 2 cut(s) 668, 1011
Alw26I GTCTC 3 cut(s) 322, 713, 973
Alw44I GTGCAC 1 cut(s) 1007
AlwI GGATC 2 cut(s) 594, 830
Aor51HI AGCGCT 1 cut(s) 1044
AoxI GGCC 4 cut(s) 358, 567, 579, 778
ApaI GGGCCC 1 cut(s) 362
ApaLI GTGCAC 1 cut(s) 1007
ApeKI GCWGC 7 cut(s) 194, 197, 370, 560, 944, 1045, 1048
ApoI RAATTY 4 cut(s) 11, 50, 130, 792
AseI ATTAAT 1 cut(s) 74
AsiGI ACCGGT 1 cut(s) 388
AspLEI GCGC 2 cut(s) 487, 1045
AspS9I GGNCC 6 cut(s) 309, 358, 359, 413, 623, 779
AsuC2I CCSGG 2 cut(s) 429, 782
AsuHPI GGTGA 3 cut(s) 209, 869, 898
AvaII GGWCC 3 cut(s) 309, 413, 623
AxyI CCTNAGG 1 cut(s) 261
BaeGI GKGCMC 2 cut(s) 362, 1011
BanII GRGCYC 1 cut(s) 362
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 2 cut(s) 668, 1011
BbvI GCAGC 7 cut(s) 206, 209, 382, 547, 956, 1032, 1060
BccI CCATC 4 cut(s) 238, 329, 511, 931
BceAI ACGGC 1 cut(s) 108
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 1 cut(s) 962
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 429, 782
BcoDI GTCTC 3 cut(s) 322, 713, 973
BfmI CTRYAG 1 cut(s) 1046
BfoI RGCGCY 1 cut(s) 1046
BglII AGATCT 1 cut(s) 699
BlpI GCTNAGC 1 cut(s) 813
BmcAI AGTACT 1 cut(s) 675
Bme1390I CCNGG 3 cut(s) 429, 782, 962
Bme18I GGWCC 3 cut(s) 309, 413, 623
BmgT120I GGNCC 6 cut(s) 309, 358, 359, 413, 623, 779
BmiI GGNNCC 3 cut(s) 360, 624, 625
BmrFI CCNGG 3 cut(s) 429, 782, 962
BmrI ACTGGG 1 cut(s) 621
BmsI GCATC 3 cut(s) 523, 628, 1063
BmuI ACTGGG 1 cut(s) 621
BoxI GACNNNNGTC 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 87
Bpu1102I GCTNAGC 1 cut(s) 813
BpuMI CCSGG 2 cut(s) 429, 782
Bsa29I ATCGAT 2 cut(s) 420, 515
BsaHI GRCGYC 2 cut(s) 279, 435
BsaJI CCNNGG 2 cut(s) 402, 582
BsaWI WCCGGW 2 cut(s) 388, 861
Bsc4I CCNNNNNNNGG 5 cut(s) 190, 306, 352, 787, 788
Bse118I RCCGGY 3 cut(s) 364, 388, 451
Bse1I ACTGG 2 cut(s) 88, 627
Bse21I CCTNAGG 1 cut(s) 261
BseBI CCWGG 1 cut(s) 962
BseCI ATCGAT 2 cut(s) 420, 515
BseDI CCNNGG 2 cut(s) 402, 582
BseGI GGATG 3 cut(s) 957, 1054, 1060
BseLI CCNNNNNNNGG 5 cut(s) 190, 306, 352, 787, 788
BseMII CTCAG 2 cut(s) 674, 858
BseNI ACTGG 2 cut(s) 88, 627
BseRI GAGGAG 2 cut(s) 490, 746
BseSI GKGCMC 2 cut(s) 362, 1011
BseXI GCAGC 7 cut(s) 206, 209, 382, 547, 956, 1032, 1060
BsgI GTGCAG 2 cut(s) 192, 389
Bsh1236I CGCG 1 cut(s) 411
Bsh1285I CGRYCG 1 cut(s) 420
BshFI GGCC 4 cut(s) 360, 569, 581, 780
BshTI ACCGGT 1 cut(s) 388
BshVI ATCGAT 2 cut(s) 420, 515
BsiEI CGRYCG 1 cut(s) 420
BsiHKAI GWGCWC 2 cut(s) 668, 1011
BsiSI CCGG 6 cut(s) 365, 389, 429, 452, 782, 862
BslFI GGGAC 3 cut(s) 144, 636, 710
BslI CCNNNNNNNGG 5 cut(s) 190, 306, 352, 787, 788
BsmAI GTCTC 3 cut(s) 322, 713, 973
BsmBI CGTCTC 2 cut(s) 322, 973
BsmFI GGGAC 3 cut(s) 144, 636, 710
BsnI GGCC 4 cut(s) 360, 569, 581, 780
Bsp120I GGGCCC 1 cut(s) 358
Bsp1286I GDGCHC 3 cut(s) 362, 668, 1011
Bsp143I GATC 7 cut(s) 214, 268, 417, 421, 586, 699, 835
Bsp1720I GCTNAGC 1 cut(s) 813
BspANI GGCC 4 cut(s) 360, 569, 581, 780
BspCNI CTCAG 2 cut(s) 673, 859
BspDI ATCGAT 2 cut(s) 420, 515
BspFNI CGCG 1 cut(s) 411
BspHI TCATGA 1 cut(s) 996
BspLI GGNNCC 3 cut(s) 360, 624, 625
BspMAI CTGCAG 1 cut(s) 1050
BspPI GGATC 2 cut(s) 594, 830
BsrFI RCCGGY 3 cut(s) 364, 388, 451
BsrI ACTGG 2 cut(s) 88, 627
BssAI RCCGGY 3 cut(s) 364, 388, 451
BssECI CCNNGG 2 cut(s) 402, 582
BssMI GATC 7 cut(s) 214, 268, 417, 421, 586, 699, 835
BssNI GRCGYC 2 cut(s) 279, 435
Bst2UI CCWGG 1 cut(s) 962
Bst4CI ACNGT 3 cut(s) 31, 724, 853
Bst6I CTCTTC 1 cut(s) 85
BstACI GRCGYC 2 cut(s) 279, 435
BstAPI GCANNNNNTGC 1 cut(s) 206
BstC8I GCNNGC 8 cut(s) 207, 362, 409, 453, 595, 757, 778, 819
BstDEI CTNAG 4 cut(s) 261, 660, 813, 867
BstEII GGTNACC 1 cut(s) 857
BstF5I GGATG 3 cut(s) 957, 1054, 1060
BstFNI CGCG 1 cut(s) 411
BstH2I RGCGCY 1 cut(s) 1046
BstHHI GCGC 2 cut(s) 487, 1045
BstKTI GATC 7 cut(s) 217, 271, 420, 424, 589, 702, 838
BstMAI GTCTC 3 cut(s) 322, 713, 973
BstMBI GATC 7 cut(s) 214, 268, 417, 421, 586, 699, 835
BstMCI CGRYCG 1 cut(s) 420
BstNI CCWGG 1 cut(s) 962
BstNSI RCATGY 1 cut(s) 604
BstPAI GACNNNNGTC 1 cut(s) 314
BstPI GGTNACC 1 cut(s) 857
BstSCI CCNGG 3 cut(s) 427, 780, 960
BstSFI CTRYAG 1 cut(s) 1046
BstSLI GKGCMC 2 cut(s) 362, 1011
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 7 cut(s) 206, 209, 382, 547, 956, 1032, 1060
BstV2I GAAGAC 1 cut(s) 87
BstX2I RGATCY 2 cut(s) 586, 699
BstYI RGATCY 2 cut(s) 586, 699
Bsu15I ATCGAT 2 cut(s) 420, 515
Bsu36I CCTNAGG 1 cut(s) 261
BsuRI GGCC 4 cut(s) 360, 569, 581, 780
BsuTUI ATCGAT 2 cut(s) 420, 515
BtgZI GCGATG 1 cut(s) 307
BtsCI GGATG 3 cut(s) 957, 1054, 1060
BtsI GCAGTG 1 cut(s) 835
BtsIMutI CAGTG 2 cut(s) 531, 835
Cac8I GCNNGC 8 cut(s) 207, 362, 409, 453, 595, 757, 778, 819
CciI TCATGA 1 cut(s) 996
CfoI GCGC 2 cut(s) 487, 1045
Cfr10I RCCGGY 3 cut(s) 364, 388, 451
Cfr13I GGNCC 6 cut(s) 309, 358, 359, 413, 623, 779
ClaI ATCGAT 2 cut(s) 420, 515
CpoI CGGWCCG 1 cut(s) 413
CseI GACGC 1 cut(s) 443
Csp6I GTAC 2 cut(s) 674, 955
CspAI ACCGGT 1 cut(s) 388
CspI CGGWCCG 1 cut(s) 413
CviAII CATG 4 cut(s) 448, 601, 983, 997
CviQI GTAC 2 cut(s) 674, 955
DdeI CTNAG 4 cut(s) 261, 660, 813, 867
DpnI GATC 7 cut(s) 216, 270, 419, 423, 588, 701, 837
DpnII GATC 7 cut(s) 214, 268, 417, 421, 586, 699, 835
DraIII CACNNNGTG 1 cut(s) 190
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
EciI GGCGGA 4 cut(s) 98, 276, 372, 470
Eco24I GRGCYC 1 cut(s) 362
Eco47I GGWCC 3 cut(s) 309, 413, 623
Eco47III AGCGCT 1 cut(s) 1044
Eco57I CTGAAG 1 cut(s) 929
Eco81I CCTNAGG 1 cut(s) 261
Eco91I GGTNACC 1 cut(s) 857
EcoO109I RGGNCCY 2 cut(s) 358, 623
EcoO65I GGTNACC 1 cut(s) 857
EcoRI GAATTC 1 cut(s) 130
EcoRII CCWGG 1 cut(s) 960
EcoT38I GRGCYC 1 cut(s) 362
Esp3I CGTCTC 2 cut(s) 322, 973
FaeI CATG 4 cut(s) 451, 604, 986, 1000
FaqI GGGAC 3 cut(s) 144, 636, 710
FatI CATG 4 cut(s) 447, 600, 982, 996
FauI CCCGC 2 cut(s) 228, 369
FbaI TGATCA 1 cut(s) 214
FokI GGATG 3 cut(s) 944, 1041, 1047
FriOI GRGCYC 1 cut(s) 362
GlaI GCGC 2 cut(s) 486, 1044
HaeII RGCGCY 1 cut(s) 1046
HaeIII GGCC 4 cut(s) 360, 569, 581, 780
HapII CCGG 6 cut(s) 365, 389, 429, 452, 782, 862
HgaI GACGC 1 cut(s) 443
HhaI GCGC 2 cut(s) 487, 1045
Hin1I GRCGYC 2 cut(s) 279, 435
Hin1II CATG 4 cut(s) 451, 604, 986, 1000
Hin6I GCGC 2 cut(s) 485, 1043
HinP1I GCGC 2 cut(s) 485, 1043
HincII GTYRAC 1 cut(s) 976
HindII GTYRAC 1 cut(s) 976
HindIII AAGCTT 1 cut(s) 815
HinfI GANTC 4 cut(s) 105, 150, 458, 521
HpaII CCGG 6 cut(s) 365, 389, 429, 452, 782, 862
HphI GGTGA 3 cut(s) 209, 869, 898
Hpy166II GTNNAC 2 cut(s) 976, 1009
Hpy188I TCNGA 7 cut(s) 241, 301, 331, 355, 417, 542, 663
Hpy188III TCNNGA 3 cut(s) 508, 997, 1037
Hpy8I GTNNAC 2 cut(s) 976, 1009
Hpy99I CGWCG 4 cut(s) 281, 284, 437, 476
HpyAV CCTTC 4 cut(s) 550, 571, 677, 904
HpyCH4III ACNGT 3 cut(s) 31, 724, 853
HpyCH4IV ACGT 2 cut(s) 279, 471
HpyCH4V TGCA 8 cut(s) 9, 209, 370, 680, 821, 828, 1009, 1048
HpyF3I CTNAG 4 cut(s) 261, 660, 813, 867
HpySE526I ACGT 2 cut(s) 279, 471
Hsp92I GRCGYC 2 cut(s) 279, 435
Hsp92II CATG 4 cut(s) 451, 604, 986, 1000
HspAI GCGC 2 cut(s) 485, 1043
KflI GGGWCCC 1 cut(s) 623
KroI GCCGGC 1 cut(s) 451
KroNI GCCGGC 1 cut(s) 453
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 7 cut(s) 214, 268, 417, 421, 586, 699, 835
LmnI GCTCC 4 cut(s) 256, 289, 733, 873
Lsp1109I GCAGC 7 cut(s) 206, 209, 382, 547, 956, 1032, 1060
LweI GCATC 3 cut(s) 523, 628, 1063
MaeII ACGT 2 cut(s) 279, 471
MaeIII GTNAC 3 cut(s) 646, 830, 857
MalI GATC 7 cut(s) 216, 270, 419, 423, 588, 701, 837
MboI GATC 7 cut(s) 214, 268, 417, 421, 586, 699, 835
MboII GAAGA 5 cut(s) 72, 87, 416, 709, 1025
MflI RGATCY 2 cut(s) 586, 699
MhlI GDGCHC 3 cut(s) 362, 668, 1011
MluCI AATT 7 cut(s) 11, 50, 71, 130, 654, 712, 792
MlyI GAGTC 2 cut(s) 99, 467
MmeI TCCRAC 1 cut(s) 333
MroNI GCCGGC 1 cut(s) 451
MseI TTAA 1 cut(s) 74
MslI CAYNNNNRTG 1 cut(s) 446
MspA1I CMGCKG 4 cut(s) 644, 776, 907, 947
MspI CCGG 6 cut(s) 365, 389, 429, 452, 782, 862
MspR9I CCNGG 3 cut(s) 429, 782, 962
MvaI CCWGG 1 cut(s) 962
MvnI CGCG 1 cut(s) 411
NaeI GCCGGC 1 cut(s) 453
NciI CCSGG 2 cut(s) 429, 782
NdeII GATC 7 cut(s) 214, 268, 417, 421, 586, 699, 835
NgoMIV GCCGGC 1 cut(s) 451
NlaIII CATG 4 cut(s) 451, 604, 986, 1000
NlaIV GGNNCC 3 cut(s) 360, 624, 625
NmeAIII GCCGAG 2 cut(s) 427, 607
NmuCI GTSAC 3 cut(s) 646, 830, 857
NspI RCATGY 1 cut(s) 604
PagI TCATGA 1 cut(s) 996
PciI ACATGT 1 cut(s) 600
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PdiI GCCGGC 1 cut(s) 453
PfeI GAWTC 2 cut(s) 150, 521
PflMI CCANNNNNTGG 1 cut(s) 190
PinAI ACCGGT 1 cut(s) 388
Ple19I CGATCG 1 cut(s) 420
PleI GAGTC 2 cut(s) 99, 466
PpsI GAGTC 2 cut(s) 99, 466
PpuMI RGGWCCY 1 cut(s) 623
PscI ACATGT 1 cut(s) 600
PshAI GACNNNNGTC 1 cut(s) 314
PshBI ATTAAT 1 cut(s) 74
PsiI TTATAA 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 623
Psp6I CCWGG 1 cut(s) 960
PspEI GGTNACC 1 cut(s) 857
PspGI CCWGG 1 cut(s) 960
PspN4I GGNNCC 3 cut(s) 360, 624, 625
PspOMI GGGCCC 1 cut(s) 358
PspPI GGNCC 6 cut(s) 309, 358, 359, 413, 623, 779
PspPPI RGGWCCY 1 cut(s) 623
PstI CTGCAG 1 cut(s) 1050
PsuI RGATCY 2 cut(s) 586, 699
PvuI CGATCG 1 cut(s) 420
PvuII CAGCTG 3 cut(s) 776, 907, 947
RsaI GTAC 2 cut(s) 675, 956
RsaNI GTAC 2 cut(s) 674, 955
RseI CAYNNNNRTG 1 cut(s) 446
Rsr2I CGGWCCG 1 cut(s) 413
RsrII CGGWCCG 1 cut(s) 413
SaqAI TTAA 1 cut(s) 74
Sau3AI GATC 7 cut(s) 214, 268, 417, 421, 586, 699, 835
Sau96I GGNCC 6 cut(s) 309, 358, 359, 413, 623, 779
ScaI AGTACT 1 cut(s) 675
SchI GAGTC 2 cut(s) 99, 467
ScrFI CCNGG 3 cut(s) 429, 782, 962
SduI GDGCHC 3 cut(s) 362, 668, 1011
SfaNI GCATC 3 cut(s) 523, 628, 1063
SfcI CTRYAG 1 cut(s) 1046
SgrAI CRCCGGYG 2 cut(s) 364, 388
SinI GGWCC 3 cut(s) 309, 413, 623
SmiMI CAYNNNNRTG 1 cut(s) 446
Sse9I AATT 7 cut(s) 11, 50, 71, 130, 654, 712, 792
StyD4I CCNGG 3 cut(s) 427, 780, 960
TaaI ACNGT 3 cut(s) 31, 724, 853
TaiI ACGT 2 cut(s) 282, 474
TaqI TCGA 4 cut(s) 267, 420, 515, 706
TaqII GACCGA 1 cut(s) 194
TasI AATT 7 cut(s) 11, 50, 71, 130, 654, 712, 792
TatI WGTACW 2 cut(s) 673, 954
TauI GCSGC 6 cut(s) 114, 376, 440, 490, 644, 1054
TfiI GAWTC 2 cut(s) 150, 521
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 2 cut(s) 531, 835
TseFI GTSAC 3 cut(s) 646, 830, 857
TseI GCWGC 7 cut(s) 194, 197, 370, 560, 944, 1045, 1048
Tsp45I GTSAC 3 cut(s) 646, 830, 857
TspDTI ATGAA 2 cut(s) 834, 985
TspGWI ACGGA 2 cut(s) 304, 493
TspRI CASTG 2 cut(s) 531, 835
Van91I CCANNNNNTGG 1 cut(s) 190
VneI GTGCAC 1 cut(s) 1007
VpaK11BI GGWCC 3 cut(s) 309, 413, 623
VspI ATTAAT 1 cut(s) 74
XapI RAATTY 4 cut(s) 11, 50, 130, 792
XceI RCATGY 1 cut(s) 604
ZraI GACGTC 1 cut(s) 280
ZrmI AGTACT 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.