Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0007919

Physical Location & Seq

Genomic Coordinates Reverse (-)

16681650 .. 16689595

Gene Structure 7946 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1782 bp

ATGGAGGGTGTGCCTTTTCACGACGGCAACATCCACGACTTGATTGAGAGACGATACGTCGACGTTCGGCACGATGCCAATCACAGGTTGCAGATGCACTCGTCGTTGGTGCGAAGGCTTTCGCAGGAAAGGGAATTGGAGGGGCATCAAGGTTGTGTTAATTCCATTGCTTGGAATTCGAATGGCTCGCTTTTAATATCTGGATCAGATGACACACGGATAAATATCTGGAACTACTCTAGTCGGAAGCTTTTGCATTCTATAGAGACTGGGCATTGTACGAACATATTTTGTACAAAATTTGTCCCTGAAACTTCTGATGAGCTCGTGGTCTCTGGAGCTGGAGATGCAGAAGTTCGATTATTTAATTTGTCTCGTTTAAATGGGAGAGGAACTGATGATAATGCTATTTCTCCATCAGCTGTGTATCAATGCCACACTAGAAGAGTAAAAAAGTTAGCTGTTGAAGTTGGAAATCCCAATGTGGTATGGAGTGCAAGTGAAGATGGAACTTTGAGACAGCATGACTTCCGAGAGGGTACTTCTTGCCCACCTGATCGATCCACTAATCAAGAATGTCGCAATGTTTTGCTTGACTTGCGGTGTGGAGCAAAGAAGTCACTGGCTGATCCCCCCAAACAAAATTTTTCTTTAAAATCTTGTGATATCAGTTCCACTAGACCTCATTTACTCCTAGTTGGCGGGAGTGATGCATTTGCACGTTTATATGATAGAAGGATGCTGCCACCACTGACATCCTGTCGGAAACGGACAGCGCCACCTCCTTGTGTTAACTATTTTTGTCCAATGCATCTCTCTGACCGTGGACGTGCTAGCTTGCATTTGACTCATGTTGCATTTAGTCCAGATGGAGAAGAGGTTCTACTTAGTTATAGTGGGGAGCATGTATATTTGATGAATGTAAATCATGCTGGTGAGAGTGTTATGCAGTATACTCCTGGAGATGTTTCGAAACTAACGAGCTTCAGTCCTCTACTCAATGGTGTCGAACTGCATCAACCAGCATCCAATGTCTTCAGAAATGATGTTCCTAAAAGGGGCAACATTGCTGCTAGGTTTGAGAAGTGCAGGAATCTAATTCAAATTGCTGAAAGATCCTTGGAGTGGACAGATTATTTTTATGGCATTGAAGTATGCAATGAGGTGTTGGATGGAAACAGTTGTGGTATTTGGCCTACACTGAAGCATGATTGCCTTTGTATTCGTGCGGGATTGTTGCTTAAGCGCAAGTGGAAAAATGATGTTCATATGGCTATAAGAGATTGTTATAATGCTAGGAGGCTCAATAGCTCCTCTTTCAGTGCTCATTACTATATGTCTGAAGCTTTATCGCAGTTGGCTAAACATAAAGAAGCTCTGGAATTTGCTATCGCAGCCCAATCTTTGGCTCCATCAAATTCTCAAGTAGCAGAACGATTGGAGCATGTAAAAAGGGATCTTGCTGCAGCTGAATCTGAAAGAAATGGTAAGCCAAATGATGGAGCACCCAGGTCTGAACCTCGAGGCGAAAGAGTACTATCACTGAGTGAAATACTCTACCGATCAGAGGGTAATAGTGATGTTTCACAAGATGGTCCAAGATCTGAAAGAGAAGATTCTGATTATGATGAGGAATTGGAGTTGGACTTTGAAACGTCGATCTCAGGTGATGAAGAGCATGACATTGAGCCCAATATTCTACAAGGAAGCTTAAACTTGAGAATTCATCGAAGAAGTGATTCTGCTAGAGAAGTTGGTGGTGCAAACTGCTCATCTGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0019538 GO:0031974 GO:0031981 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043687 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0070013 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

594

Amino Acids

66.37

Weight (kDa)

6.07

Isoelectric Point (pI)

56.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	27 - 125	4.1e-10	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	32 - 126	8.3e-08	TEP-1 second beta-propeller
ANAPC4_WD40	PF12894	37 - 98	4.5e-06	Anaphase-promoting complex subunit 4 WD40 domain
Beta-prop_WDR5	PF25175	38 - 181	5.2e-19	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	40 - 97	6.2e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	41 - 125	2.5e-13	WDR3 first beta-propeller domain
WDR55	PF24796	42 - 180	5.7e-07	WDR55
WD40	PF00400	43 - 78	7.5e-10	WD domain, G-beta repeat
EIF3I	PF24805	46 - 125	1.2e-06	EIF3I
Beta-prop_THOC3	PF25174	49 - 125	1.5e-11	THOC3 beta-propeller domain
WD40_Prp19	PF24814	84 - 182	9.9e-06	Prp19 WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0007919)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10940 AT5G10940
fragaria_vesca	FvH4_5g35830 FvH4_5g35830 FvH4_5g35830
malus_domestica	MD08G1192800.v1.1 MD15G1381500.v1.1
prunus_persica	Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1 Prupe.1G527400_v2.0.a1
pyrus_communis	pycom08g16630 pycom15g34170
rosa_chinensis	RchiOBHm_Chr7g0237521
rosa_laevigata	RLG00000000996
rosa_multiflora	Rmu_ssc0000152.1_g000001
rosa_roxburghii	Rroxscaffold_3G00224060
rosa_rugosa	Rorug07G0303800 Rorug07G0303900
rosa_samantha	Rh7AG461500 Rh7BG431700 Rh7CG478700 Rh7DG447900
rosa_wichuraiana	Rw7G038190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1290
AbsI	CCTCGAGG	1 cut(s)	1521
AccB7I	CCANNNNNTGG	3 cut(s)	171, 1405, 1499
AccI	GTMKAC	2 cut(s)	60, 953
AciI	CCGC	3 cut(s)	601, 702, 1229
AclWI	GGATC	5 cut(s)	211, 555, 623, 1110, 1464
AcsI	RAATTY	6 cut(s)	175, 299, 643, 1382, 1417, 1722
AcuI	CTGAAG	4 cut(s)	970, 1021, 1223, 1362
AdeI	CACNNNGTG	1 cut(s)	1547
AfaI	GTAC	4 cut(s)	280, 295, 541, 1536
AfiI	CCNNNNNNNGG	7 cut(s)	84, 171, 1058, 1125, 1405, 1499, 1567
AflII	CTTAAG	1 cut(s)	1241
AgsI	TTSAA	4 cut(s)	467, 1103, 1151, 1652
AhdI	GACNNNNNGTC	2 cut(s)	56, 759
AjiI	CACGTC	1 cut(s)	830
AjnI	CCWGG	2 cut(s)	958, 1508
AloI	GAACNNNNNNTCC	2 cut(s)	864, 896
Alw21I	GWGCWC	3 cut(s)	327, 1327, 1507
Alw26I	GTCTC	5 cut(s)	43, 260, 337, 378, 511
AlwI	GGATC	5 cut(s)	211, 555, 623, 1110, 1464
Ama87I	CYCGRG	1 cut(s)	1521
AoxI	GGCC	1 cut(s)	1193
ApeKI	GCWGC	5 cut(s)	742, 1070, 1394, 1463, 1466
ApoI	RAATTY	6 cut(s)	175, 299, 643, 1382, 1417, 1722
Asp700I	GAANNNNTTC	3 cut(s)	118, 879, 1738
AspLEI	GCGC	2 cut(s)	778, 1248
AspS9I	GGNCC	1 cut(s)	1595
AsuHPI	GGTGA	2 cut(s)	947, 1679
AsuII	TTCGAA	2 cut(s)	179, 971
AsuNHI	GCTAGC	1 cut(s)	833
AvaI	CYCGRG	1 cut(s)	1521
AvaII	GGWCC	1 cut(s)	1595
BanII	GRGCYC	2 cut(s)	327, 1692
BarI	GAAGNNNNNNTAC	2 cut(s)	867, 899
BauI	CACGAG	1 cut(s)	326
BbsI	GAAGAC	1 cut(s)	1027
Bbv12I	GWGCWC	3 cut(s)	327, 1327, 1507
BbvI	GCAGC	5 cut(s)	729, 1057, 1406, 1450, 1478
BccI	CCATC	7 cut(s)	424, 500, 863, 1166, 1420, 1493, 1586
BceAI	ACGGC	1 cut(s)	40
BciT130I	CCWGG	2 cut(s)	960, 1510
BcoDI	GTCTC	5 cut(s)	43, 260, 337, 378, 511
BfaI	CTAG	8 cut(s)	240, 441, 678, 695, 834, 1074, 1296, 1746
BfmI	CTRYAG	2 cut(s)	261, 1464
BfoI	RGCGCY	1 cut(s)	779
BfrI	CTTAAG	1 cut(s)	1241
BglII	AGATCT	1 cut(s)	1601
BisI	GCNGC	5 cut(s)	743, 1071, 1395, 1464, 1467
BlsI	GCNGC	5 cut(s)	744, 1072, 1396, 1465, 1468
BmcAI	AGTACT	1 cut(s)	1536
Bme1390I	CCNGG	2 cut(s)	960, 1510
Bme18I	GGWCC	1 cut(s)	1595
BmeRI	GACNNNNNGTC	2 cut(s)	56, 759
BmeT110I	CYCGRG	1 cut(s)	1521
BmgBI	CACGTC	1 cut(s)	830
BmgT120I	GGNCC	1 cut(s)	1595
BmiI	GGNNCC	1 cut(s)	1410
BmrFI	CCNGG	2 cut(s)	960, 1510
BmrI	ACTGGG	1 cut(s)	279
BmsI	GCATC	9 cut(s)	64, 84, 154, 337, 700, 729, 820, 1024, 1034
BmtI	GCTAGC	1 cut(s)	837
BmuI	ACTGGG	1 cut(s)	279
BpiI	GAAGAC	1 cut(s)	1027
BpmI	CTGGAG	3 cut(s)	357, 363, 981
Bpu14I	TTCGAA	2 cut(s)	179, 971
BpuEI	CTTGAG	2 cut(s)	1407, 1738
Bsa29I	ATCGAT	1 cut(s)	559
BsaBI	GATNNNNATC	2 cut(s)	78, 224
BsaI	GGTCTC	1 cut(s)	337
BsaJI	CCNNGG	3 cut(s)	823, 1119, 1508
BsaXI	ACNNNNNCTCC	4 cut(s)	484, 514, 864, 894
Bsc4I	CCNNNNNNNGG	7 cut(s)	84, 171, 1058, 1125, 1405, 1499, 1567
Bse1I	ACTGG	2 cut(s)	274, 627
Bse3DI	GCAATG	4 cut(s)	165, 589, 1065, 1165
Bse8I	GATNNNNATC	2 cut(s)	78, 224
BseBI	CCWGG	2 cut(s)	960, 1510
BseCI	ATCGAT	1 cut(s)	559
BseDI	CCNNGG	3 cut(s)	823, 1119, 1508
BseGI	GGATG	5 cut(s)	30, 744, 755, 1025, 1177
BseJI	GATNNNNATC	2 cut(s)	78, 224
BseLI	CCNNNNNNNGG	7 cut(s)	84, 171, 1058, 1125, 1405, 1499, 1567
BseMI	GCAATG	4 cut(s)	165, 589, 1065, 1165
BseMII	CTCAG	2 cut(s)	1535, 1677
BseNI	ACTGG	2 cut(s)	274, 627
BseRI	GAGGAG	1 cut(s)	1303
BseXI	GCAGC	5 cut(s)	729, 1057, 1406, 1450, 1478
BsgI	GTGCAG	1 cut(s)	1108
BshFI	GGCC	1 cut(s)	1195
BshVI	ATCGAT	1 cut(s)	559
BsiHKAI	GWGCWC	3 cut(s)	327, 1327, 1507
BsiHKCI	CYCGRG	1 cut(s)	1521
BslFI	GGGAC	1 cut(s)	290
BslI	CCNNNNNNNGG	7 cut(s)	84, 171, 1058, 1125, 1405, 1499, 1567
BsmAI	GTCTC	5 cut(s)	43, 260, 337, 378, 511
BsmBI	CGTCTC	1 cut(s)	43
BsmFI	GGGAC	1 cut(s)	290
BsmI	GAATGC	1 cut(s)	256
BsnI	GGCC	1 cut(s)	1195
Bso31I	GGTCTC	1 cut(s)	337
BsoBI	CYCGRG	1 cut(s)	1521
Bsp119I	TTCGAA	2 cut(s)	179, 971
Bsp1286I	GDGCHC	4 cut(s)	327, 1327, 1507, 1692
Bsp1407I	TGTACA	1 cut(s)	293
Bsp143I	GATC	9 cut(s)	203, 556, 560, 628, 1115, 1456, 1562, 1601, 1659
BspACI	CCGC	3 cut(s)	601, 702, 1229
BspANI	GGCC	1 cut(s)	1195
BspCNI	CTCAG	2 cut(s)	1536, 1676
BspDI	ATCGAT	1 cut(s)	559
BspLI	GGNNCC	1 cut(s)	1410
BspMAI	CTGCAG	1 cut(s)	1468
BspOI	GCTAGC	1 cut(s)	837
BspPI	GGATC	5 cut(s)	211, 555, 623, 1110, 1464
BspQI	GCTCTTC	1 cut(s)	1668
BspT104I	TTCGAA	2 cut(s)	179, 971
BspTI	CTTAAG	1 cut(s)	1241
BspTNI	GGTCTC	1 cut(s)	337
BsrDI	GCAATG	4 cut(s)	165, 589, 1065, 1165
BsrGI	TGTACA	1 cut(s)	293
BsrI	ACTGG	2 cut(s)	274, 627
BssECI	CCNNGG	3 cut(s)	823, 1119, 1508
BssMI	GATC	9 cut(s)	203, 556, 560, 628, 1115, 1456, 1562, 1601, 1659
BssNAI	GTATAC	1 cut(s)	954
BssSI	CACGAG	1 cut(s)	326
BssT1I	CCWWGG	1 cut(s)	1119
Bst1107I	GTATAC	1 cut(s)	954
Bst2BI	CACGAG	1 cut(s)	326
Bst2UI	CCWGG	2 cut(s)	960, 1510
Bst4CI	ACNGT	2 cut(s)	824, 1181
Bst6I	CTCTTC	3 cut(s)	439, 870, 1668
BstAFI	CTTAAG	1 cut(s)	1241
BstAUI	TGTACA	1 cut(s)	293
BstBI	TTCGAA	2 cut(s)	179, 971
BstC8I	GCNNGC	3 cut(s)	188, 835, 839
BstDEI	CTNAG	3 cut(s)	887, 1544, 1663
BstDSI	CCRYGG	1 cut(s)	823
BstF5I	GGATG	5 cut(s)	30, 744, 755, 1025, 1177
BstH2I	RGCGCY	1 cut(s)	779
BstHHI	GCGC	2 cut(s)	778, 1248
BstKTI	GATC	9 cut(s)	206, 559, 563, 631, 1118, 1459, 1565, 1604, 1662
BstMAI	GTCTC	5 cut(s)	43, 260, 337, 378, 511
BstMBI	GATC	9 cut(s)	203, 556, 560, 628, 1115, 1456, 1562, 1601, 1659
BstMWI	GCNNNNNNNGC	3 cut(s)	347, 598, 1394
BstNI	CCWGG	2 cut(s)	960, 1510
BstNSI	RCATGY	2 cut(s)	908, 1448
BstSCI	CCNGG	2 cut(s)	958, 1508
BstSFI	CTRYAG	2 cut(s)	261, 1464
BstV1I	GCAGC	5 cut(s)	729, 1057, 1406, 1450, 1478
BstV2I	GAAGAC	1 cut(s)	1027
BstX2I	RGATCY	3 cut(s)	1115, 1456, 1601
BstYI	RGATCY	3 cut(s)	1115, 1456, 1601
BstZ17I	GTATAC	1 cut(s)	954
Bsu15I	ATCGAT	1 cut(s)	559
BsuRI	GGCC	1 cut(s)	1195
BsuTUI	ATCGAT	1 cut(s)	559
BtgI	CCRYGG	1 cut(s)	823
BtrI	CACGTC	1 cut(s)	830
BtsCI	GGATG	5 cut(s)	30, 744, 755, 1025, 1177
BtsIMutI	CAGTG	5 cut(s)	620, 749, 1199, 1327, 1541
Cac8I	GCNNGC	3 cut(s)	188, 835, 839
CfoI	GCGC	2 cut(s)	778, 1248
Cfr13I	GGNCC	1 cut(s)	1595
ClaI	ATCGAT	1 cut(s)	559
Csp6I	GTAC	4 cut(s)	279, 294, 540, 1535
CviAII	CATG	7 cut(s)	524, 851, 905, 929, 1208, 1445, 1679
CviQI	GTAC	4 cut(s)	279, 294, 540, 1535
DdeI	CTNAG	3 cut(s)	887, 1544, 1663
DpnI	GATC	9 cut(s)	205, 558, 562, 630, 1117, 1458, 1564, 1603, 1661
DpnII	GATC	9 cut(s)	203, 556, 560, 628, 1115, 1456, 1562, 1601, 1659
DraI	TTTAAA	2 cut(s)	381, 654
DraIII	CACNNNGTG	1 cut(s)	1547
DriI	GACNNNNNGTC	2 cut(s)	56, 759
Eam1104I	CTCTTC	3 cut(s)	439, 870, 1668
Eam1105I	GACNNNNNGTC	2 cut(s)	56, 759
EarI	CTCTTC	3 cut(s)	439, 870, 1668
Ecl136II	GAGCTC	1 cut(s)	325
Eco130I	CCWWGG	1 cut(s)	1119
Eco24I	GRGCYC	2 cut(s)	327, 1692
Eco31I	GGTCTC	1 cut(s)	337
Eco32I	GATATC	1 cut(s)	667
Eco47I	GGWCC	1 cut(s)	1595
Eco53kI	GAGCTC	1 cut(s)	325
Eco57I	CTGAAG	4 cut(s)	970, 1021, 1223, 1362
Eco88I	CYCGRG	1 cut(s)	1521
EcoICRI	GAGCTC	1 cut(s)	325
EcoRI	GAATTC	2 cut(s)	175, 1722
EcoRII	CCWGG	2 cut(s)	958, 1508
EcoRV	GATATC	1 cut(s)	667
EcoT14I	CCWWGG	1 cut(s)	1119
EcoT22I	ATGCAT	2 cut(s)	715, 813
EcoT38I	GRGCYC	2 cut(s)	327, 1692
ErhI	CCWWGG	1 cut(s)	1119
Esp3I	CGTCTC	1 cut(s)	43
FaeI	CATG	7 cut(s)	527, 854, 908, 932, 1211, 1448, 1682
FaqI	GGGAC	1 cut(s)	290
FatI	CATG	7 cut(s)	523, 850, 904, 928, 1207, 1444, 1678
FauI	CCCGC	2 cut(s)	695, 1222
FauNDI	CATATG	1 cut(s)	1269
FblI	GTMKAC	2 cut(s)	60, 953
Fnu4HI	GCNGC	5 cut(s)	743, 1071, 1395, 1464, 1467
FokI	GGATG	5 cut(s)	17, 742, 751, 1012, 1184
FriOI	GRGCYC	2 cut(s)	327, 1692
Fsp4HI	GCNGC	5 cut(s)	743, 1071, 1395, 1464, 1467
FspBI	CTAG	8 cut(s)	240, 441, 678, 695, 834, 1074, 1296, 1746
GlaI	GCGC	2 cut(s)	777, 1247
GluI	GCNGC	5 cut(s)	743, 1071, 1395, 1464, 1467
GsuI	CTGGAG	3 cut(s)	357, 363, 981
HaeII	RGCGCY	1 cut(s)	779
HaeIII	GGCC	1 cut(s)	1195
HhaI	GCGC	2 cut(s)	778, 1248
Hin1II	CATG	7 cut(s)	527, 854, 908, 932, 1211, 1448, 1682
Hin6I	GCGC	2 cut(s)	776, 1246
HinP1I	GCGC	2 cut(s)	776, 1246
HincII	GTYRAC	2 cut(s)	61, 793
HindII	GTYRAC	2 cut(s)	61, 793
HindIII	AAGCTT	3 cut(s)	248, 1344, 1708
HinfI	GANTC	5 cut(s)	847, 1093, 1472, 1616, 1739
HpaI	GTTAAC	1 cut(s)	793
HphI	GGTGA	2 cut(s)	947, 1679
Hpy166II	GTNNAC	5 cut(s)	61, 793, 827, 954, 1128
Hpy188III	TCNNGA	7 cut(s)	20, 201, 229, 336, 572, 866, 1379
Hpy8I	GTNNAC	5 cut(s)	61, 793, 827, 954, 1128
Hpy99I	CGWCG	5 cut(s)	26, 62, 65, 106, 1660
HpyAV	CCTTC	2 cut(s)	108, 729
HpyCH4III	ACNGT	2 cut(s)	824, 1181
HpyCH4IV	ACGT	5 cut(s)	57, 63, 721, 829, 1655
HpyF10VI	GCNNNNNNNGC	3 cut(s)	347, 598, 1394
HpyF3I	CTNAG	3 cut(s)	887, 1544, 1663
HpySE526I	ACGT	5 cut(s)	57, 63, 721, 829, 1655
Hsp92II	CATG	7 cut(s)	527, 854, 908, 932, 1211, 1448, 1682
HspAI	GCGC	2 cut(s)	776, 1246
KspAI	GTTAAC	1 cut(s)	793
Kzo9I	GATC	9 cut(s)	203, 556, 560, 628, 1115, 1456, 1562, 1601, 1659
LguI	GCTCTTC	1 cut(s)	1668
LmnI	GCTCC	7 cut(s)	338, 608, 901, 1316, 1414, 1441, 1502
Lsp1109I	GCAGC	5 cut(s)	729, 1057, 1406, 1450, 1478
LweI	GCATC	9 cut(s)	64, 84, 154, 337, 700, 729, 820, 1024, 1034
MaeI	CTAG	8 cut(s)	240, 441, 678, 695, 834, 1074, 1296, 1746
MaeII	ACGT	5 cut(s)	57, 63, 721, 829, 1655
MaeIII	GTNAC	1 cut(s)	618
MalI	GATC	9 cut(s)	205, 558, 562, 630, 1117, 1458, 1564, 1603, 1661
MboI	GATC	9 cut(s)	203, 556, 560, 628, 1115, 1456, 1562, 1601, 1659
MboII	GAAGA	7 cut(s)	456, 515, 887, 1027, 1625, 1685, 1743
MflI	RGATCY	3 cut(s)	1115, 1456, 1601
MhlI	GDGCHC	4 cut(s)	327, 1327, 1507, 1692
MlyI	GAGTC	1 cut(s)	841
MmeI	TCCRAC	5 cut(s)	224, 451, 743, 1149, 1623
Mph1103I	ATGCAT	2 cut(s)	715, 813
MroXI	GAANNNNTTC	3 cut(s)	118, 879, 1738
MseI	TTAA	8 cut(s)	159, 194, 366, 380, 653, 792, 1242, 1712
MslI	CAYNNNNRTG	1 cut(s)	933
MspA1I	CMGCKG	2 cut(s)	422, 1469
MspCI	CTTAAG	1 cut(s)	1241
MspR9I	CCNGG	2 cut(s)	960, 1510
Mva1269I	GAATGC	1 cut(s)	256
MvaI	CCWGG	2 cut(s)	960, 1510
MwoI	GCNNNNNNNGC	3 cut(s)	347, 598, 1394
NdeI	CATATG	1 cut(s)	1269
NdeII	GATC	9 cut(s)	203, 556, 560, 628, 1115, 1456, 1562, 1601, 1659
NheI	GCTAGC	1 cut(s)	833
NlaIII	CATG	7 cut(s)	527, 854, 908, 932, 1211, 1448, 1682
NlaIV	GGNNCC	1 cut(s)	1410
NmuCI	GTSAC	1 cut(s)	618
NsiI	ATGCAT	2 cut(s)	715, 813
NspI	RCATGY	2 cut(s)	908, 1448
NspV	TTCGAA	2 cut(s)	179, 971
PaeR7I	CTCGAG	1 cut(s)	1521
PciSI	GCTCTTC	1 cut(s)	1668
PcsI	WCGNNNNNNNCGW	2 cut(s)	69, 977
PctI	GAATGC	1 cut(s)	256
PdmI	GAANNNNTTC	3 cut(s)	118, 879, 1738
PfeI	GAWTC	4 cut(s)	1093, 1472, 1616, 1739
PflMI	CCANNNNNTGG	3 cut(s)	171, 1405, 1499
PfoI	TCCNGGA	1 cut(s)	958
PkrI	GCNGC	5 cut(s)	744, 1072, 1396, 1465, 1468
PleI	GAGTC	1 cut(s)	841
PpsI	GAGTC	1 cut(s)	841
PsiI	TTATAA	1 cut(s)	1290
Psp124BI	GAGCTC	1 cut(s)	327
Psp6I	CCWGG	2 cut(s)	958, 1508
PspGI	CCWGG	2 cut(s)	958, 1508
PspN4I	GGNNCC	1 cut(s)	1410
PspPI	GGNCC	1 cut(s)	1595
PspXI	VCTCGAGB	1 cut(s)	1521
PstI	CTGCAG	1 cut(s)	1468
PsuI	RGATCY	3 cut(s)	1115, 1456, 1601
PvuII	CAGCTG	2 cut(s)	422, 1469
RsaI	GTAC	4 cut(s)	280, 295, 541, 1536
RsaNI	GTAC	4 cut(s)	279, 294, 540, 1535
RseI	CAYNNNNRTG	1 cut(s)	933
SacI	GAGCTC	1 cut(s)	327
SalI	GTCGAC	1 cut(s)	59
SapI	GCTCTTC	1 cut(s)	1668
SaqAI	TTAA	8 cut(s)	159, 194, 366, 380, 653, 792, 1242, 1712
SatI	GCNGC	5 cut(s)	743, 1071, 1395, 1464, 1467
Sau3AI	GATC	9 cut(s)	203, 556, 560, 628, 1115, 1456, 1562, 1601, 1659
Sau96I	GGNCC	1 cut(s)	1595
ScaI	AGTACT	1 cut(s)	1536
SchI	GAGTC	1 cut(s)	841
ScrFI	CCNGG	2 cut(s)	960, 1510
SduI	GDGCHC	4 cut(s)	327, 1327, 1507, 1692
SfaNI	GCATC	9 cut(s)	64, 84, 154, 337, 700, 729, 820, 1024, 1034
SfcI	CTRYAG	2 cut(s)	261, 1464
Sfr274I	CTCGAG	1 cut(s)	1521
SfuI	TTCGAA	2 cut(s)	179, 971
SgrDI	CGTCGACG	1 cut(s)	59
SinI	GGWCC	1 cut(s)	1595
SlaI	CTCGAG	1 cut(s)	1521
SmiMI	CAYNNNNRTG	1 cut(s)	933
SmlI	CTYRAG	4 cut(s)	1241, 1422, 1521, 1717
SmoI	CTYRAG	4 cut(s)	1241, 1422, 1521, 1717
SsiI	CCGC	3 cut(s)	601, 702, 1229
SspI	AATATT	1 cut(s)	1696
SspMI	CTAG	8 cut(s)	240, 441, 678, 695, 834, 1074, 1296, 1746
SstI	GAGCTC	1 cut(s)	327
StyD4I	CCNGG	2 cut(s)	958, 1508
StyI	CCWWGG	1 cut(s)	1119
TaaI	ACNGT	2 cut(s)	824, 1181
TaiI	ACGT	5 cut(s)	60, 66, 724, 832, 1658
TaqI	TCGA	9 cut(s)	60, 179, 358, 559, 971, 1008, 1522, 1658, 1729
TatI	WGTACW	2 cut(s)	293, 1534
TfiI	GAWTC	4 cut(s)	1093, 1472, 1616, 1739
Tru1I	TTAA	8 cut(s)	159, 194, 366, 380, 653, 792, 1242, 1712
Tru9I	TTAA	8 cut(s)	159, 194, 366, 380, 653, 792, 1242, 1712
TscAI	CASTG	5 cut(s)	627, 756, 1206, 1327, 1548
TseFI	GTSAC	1 cut(s)	618
TseI	GCWGC	5 cut(s)	742, 1070, 1394, 1463, 1466
Tsp45I	GTSAC	1 cut(s)	618
TspDTI	ATGAA	4 cut(s)	932, 1256, 1686, 1715
TspGWI	ACGGA	2 cut(s)	232, 784
TspRI	CASTG	5 cut(s)	627, 756, 1206, 1327, 1548
Van91I	CCANNNNNTGG	3 cut(s)	171, 1405, 1499
Vha464I	CTTAAG	1 cut(s)	1241
VpaK11BI	GGWCC	1 cut(s)	1595
XapI	RAATTY	6 cut(s)	175, 299, 643, 1382, 1417, 1722
XceI	RCATGY	2 cut(s)	908, 1448
XhoI	CTCGAG	1 cut(s)	1521
XmiI	GTMKAC	2 cut(s)	60, 953
XmnI	GAANNNNTTC	3 cut(s)	118, 879, 1738
XspI	CTAG	8 cut(s)	240, 441, 678, 695, 834, 1074, 1296, 1746
ZrmI	AGTACT	1 cut(s)	1536
Zsp2I	ATGCAT	2 cut(s)	715, 813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.