pycom08g20200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0010190
Physical Location & Seq
Reverse (-)
20075346 .. 20078903
3558 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1038 bp
ATGAAAGTTCTACCAAAGTTAGCCAAAAGAACAATTTTTTCGTTAAAATTCTGTTTATTATATCGTAATATATCGAGACGTACAGTTGACAGAAACGAACCAAACACTGTAAATTTGGAGGGGACGATTGGAGAACCAGAGAAGGGGAGGAAGATGAAGAAGAGGACAGTGTACGCTTGGGGAGTAGCCATTCTTTGCTTTGTGGTGTTGATGATCGTCACTCCTGCCATCCCCCAGTCTCAGGCTTACCACGATTTCGCTGACCAACGCGAGTTTTTCGGTATTCCAAATACACTTAATGTGGTTTCAAATTTTCCTTTTCTTGTTATTGGTCTCATTGGTCTTTTCCTCTGCTATTACAAGGATTATTTCAAGTTCAGCTTGCGAGGAGAGCTTTGGGGTTGGACATGCTTTTACATCGGTGTAGCTGCTGTTGCAATTGGATCATCATACTATCACCTGAAGCCAAATGATGCCCGTCTAGTGTGGGACCGCTTGCCAATGACAATTGCATTCACATCGATCATTGCAATCTTTATCATTGAAAGGATTGATGAGTGGAAGGGAACAATTTCCATCATACCTCTACTTTTGGCGGGTGTAATCAGCATATTGTATTGGAGGTTCTTTGATGACCTCCGTCCATATGCTATGGTCCAGTTTCTTCCTTGCATTGCCATTCCTCTAATGGCTATCTTGTTGCCCCCAATGTACACTCATTCCACATATTGGCTTTGGGCAGCTGGATTTTATCTTTTAGCTAAGGTGGAAGAAGCTGCTGATAAAGTGATCTACGATTGGACTCATCATATTGTCAGCGGGCACACCCTTAAGCATTTGTGCGCTGCAATGGTTCCTGTATTCTTGACACTTATGCTTGCAAAGAGGACTGTTGAACCAGAGAGGCAAAGTTTGCTCCAGGTTTGGAAGATTTCCTGGACTAATTTTAGGAAGAAAGATGGCTCAGTAGAGAGTGATGGCACTGTGGAGAGTTACACGTGTACCTACTCAACAGTTCCGGTTGAGGAATCACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ceramidase

Protein Analysis

346

Amino Acids

39.7

Weight (kDa)

9.02

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ceramidase PF05875 98 - 212 5e-09 Ceramidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010190)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71190 AT1G71190
fragaria_vesca FvH4_5g29250 FvH4_5g29250 FvH4_5g29250 FvH4_5g29250 FvH4_5g29250
malus_domestica MD08G1233100.v1.1
prunus_persica Prupe.1G571000_v2.0.a1
pyrus_communis pycom08g20200
rosa_chinensis RchiOBHm_Chr7g0222011
rosa_laevigata RLG00000002103
rosa_multiflora Rmu_sc0004325.1_g000004
rosa_roxburghii Rroxscaffold_3G00237500
rosa_rugosa Rorug07G0204900 Rorug07G0205000
rosa_samantha Rh7AG345500 Rh7BG336000 Rh7CG363200 Rh7DG343800
rosa_wichuraiana Rw7G029300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 729
AccII CGCG 1 cut(s) 270
AciI CCGC 3 cut(s) 493, 596, 819
AclWI GGATC 1 cut(s) 451
AcsI RAATTY 3 cut(s) 47, 112, 310
AcuI CTGAAG 1 cut(s) 482
AcvI CACGTG 1 cut(s) 999
AfaI GTAC 4 cut(s) 82, 173, 713, 1003
AfiI CCNNNNNNNGG 3 cut(s) 143, 241, 729
AflII CTTAAG 1 cut(s) 830
AflIII ACRYGT 2 cut(s) 996, 998
AgsI TTSAA 4 cut(s) 309, 373, 545, 896
AjnI CCWGG 2 cut(s) 918, 935
AluBI AGCT 6 cut(s) 381, 394, 428, 743, 761, 776
AluI AGCT 6 cut(s) 381, 394, 428, 743, 761, 776
Alw26I GTCTC 3 cut(s) 70, 243, 338
AlwI GGATC 1 cut(s) 451
ApeKI GCWGC 4 cut(s) 428, 740, 776, 845
ApoI RAATTY 3 cut(s) 47, 112, 310
Asp700I GAANNNNTTC 1 cut(s) 571
AspLEI GCGC 1 cut(s) 845
AspS9I GGNCC 2 cut(s) 490, 655
AsuHPI GGTGA 1 cut(s) 449
AvaII GGWCC 2 cut(s) 490, 655
BaeGI GKGCMC 1 cut(s) 825
BaeI ACNNNNGTAYC 2 cut(s) 985, 1018
BbrPI CACGTG 1 cut(s) 999
BbvI GCAGC 4 cut(s) 415, 752, 763, 832
BccI CCATC 4 cut(s) 236, 584, 953, 971
BcgI CGANNNNNNTGC 2 cut(s) 501, 535
BciT130I CCWGG 2 cut(s) 920, 937
BcoDI GTCTC 3 cut(s) 70, 243, 338
BfaI CTAG 1 cut(s) 482
BfrI CTTAAG 1 cut(s) 830
BisI GCNGC 4 cut(s) 429, 741, 777, 846
BlsI GCNGC 4 cut(s) 430, 742, 778, 847
Bme1390I CCNGG 2 cut(s) 920, 937
Bme18I GGWCC 2 cut(s) 490, 655
BmgT120I GGNCC 2 cut(s) 490, 655
BmiI GGNNCC 2 cut(s) 491, 855
BmrFI CCNGG 2 cut(s) 920, 937
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 1 cut(s) 463
BmuI ACTGGG 1 cut(s) 229
BoxI GACNNNNGTC 1 cut(s) 639
BpmI CTGGAG 1 cut(s) 902
Bpu10I CCTNAGC 1 cut(s) 762
Bsa29I ATCGAT 1 cut(s) 521
BsaAI YACGTR 1 cut(s) 999
BsaI GGTCTC 1 cut(s) 338
BsaWI WCCGGW 1 cut(s) 1018
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 241, 729
Bse1I ACTGG 2 cut(s) 235, 658
Bse3DI GCAATG 3 cut(s) 525, 672, 855
BseBI CCWGG 2 cut(s) 920, 937
BseCI ATCGAT 1 cut(s) 521
BseGI GGATG 1 cut(s) 228
BseLI CCNNNNNNNGG 3 cut(s) 143, 241, 729
BseMI GCAATG 3 cut(s) 525, 672, 855
BseMII CTCAG 2 cut(s) 254, 978
BseNI ACTGG 2 cut(s) 235, 658
BseRI GAGGAG 1 cut(s) 402
BseSI GKGCMC 1 cut(s) 825
BseXI GCAGC 4 cut(s) 415, 752, 763, 832
Bsh1236I CGCG 1 cut(s) 270
BshVI ATCGAT 1 cut(s) 521
BsiSI CCGG 1 cut(s) 1019
BslFI GGGAC 2 cut(s) 136, 503
BslI CCNNNNNNNGG 3 cut(s) 143, 241, 729
BsmAI GTCTC 3 cut(s) 70, 243, 338
BsmBI CGTCTC 1 cut(s) 70
BsmFI GGGAC 2 cut(s) 136, 503
BsmI GAATGC 1 cut(s) 512
Bso31I GGTCTC 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 825
Bsp1407I TGTACA 1 cut(s) 711
Bsp143I GATC 4 cut(s) 213, 443, 522, 789
BspACI CCGC 3 cut(s) 493, 596, 819
BspCNI CTCAG 2 cut(s) 253, 977
BspDI ATCGAT 1 cut(s) 521
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 2 cut(s) 491, 855
BspPI GGATC 1 cut(s) 451
BspTI CTTAAG 1 cut(s) 830
BspTNI GGTCTC 1 cut(s) 338
BsrDI GCAATG 3 cut(s) 525, 672, 855
BsrGI TGTACA 1 cut(s) 711
BsrI ACTGG 2 cut(s) 235, 658
BssMI GATC 4 cut(s) 213, 443, 522, 789
Bst2UI CCWGG 2 cut(s) 920, 937
Bst4CI ACNGT 6 cut(s) 85, 109, 169, 892, 985, 1015
Bst6I CTCTTC 1 cut(s) 155
BstAFI CTTAAG 1 cut(s) 830
BstAPI GCANNNNNTGC 1 cut(s) 913
BstAUI TGTACA 1 cut(s) 711
BstBAI YACGTR 1 cut(s) 999
BstC8I GCNNGC 4 cut(s) 383, 497, 821, 879
BstDEI CTNAG 3 cut(s) 240, 762, 964
BstF5I GGATG 1 cut(s) 228
BstFNI CGCG 1 cut(s) 270
BstHHI GCGC 1 cut(s) 845
BstKTI GATC 4 cut(s) 216, 446, 525, 792
BstMAI GTCTC 3 cut(s) 70, 243, 338
BstMBI GATC 4 cut(s) 213, 443, 522, 789
BstMWI GCNNNNNNNGC 3 cut(s) 391, 434, 913
BstNI CCWGG 2 cut(s) 920, 937
BstNSI RCATGY 1 cut(s) 411
BstPAI GACNNNNGTC 1 cut(s) 639
BstSCI CCNGG 2 cut(s) 918, 935
BstSLI GKGCMC 1 cut(s) 825
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 4 cut(s) 415, 752, 763, 832
Bsu15I ATCGAT 1 cut(s) 521
BsuTUI ATCGAT 1 cut(s) 521
BtsCI GGATG 1 cut(s) 228
BtsIMutI CAGTG 3 cut(s) 105, 174, 981
Cac8I GCNNGC 4 cut(s) 383, 497, 821, 879
CfoI GCGC 1 cut(s) 845
Cfr13I GGNCC 2 cut(s) 490, 655
ClaI ATCGAT 1 cut(s) 521
Csp6I GTAC 4 cut(s) 81, 172, 712, 1002
CviAII CATG 1 cut(s) 408
CviQI GTAC 4 cut(s) 81, 172, 712, 1002
DdeI CTNAG 3 cut(s) 240, 762, 964
DpnI GATC 4 cut(s) 215, 445, 524, 791
DpnII GATC 4 cut(s) 213, 443, 522, 789
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
Eco31I GGTCTC 1 cut(s) 338
Eco47I GGWCC 2 cut(s) 490, 655
Eco57I CTGAAG 1 cut(s) 482
Eco72I CACGTG 1 cut(s) 999
EcoRII CCWGG 2 cut(s) 918, 935
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 1 cut(s) 411
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FaqI GGGAC 2 cut(s) 136, 503
FatI CATG 1 cut(s) 407
FauI CCCGC 2 cut(s) 589, 812
FauNDI CATATG 1 cut(s) 646
Fnu4HI GCNGC 4 cut(s) 429, 741, 777, 846
FokI GGATG 1 cut(s) 215
Fsp4HI GCNGC 4 cut(s) 429, 741, 777, 846
FspBI CTAG 1 cut(s) 482
GlaI GCGC 1 cut(s) 844
GluI GCNGC 4 cut(s) 429, 741, 777, 846
GsuI CTGGAG 1 cut(s) 902
HapII CCGG 1 cut(s) 1019
HhaI GCGC 1 cut(s) 845
Hin1II CATG 1 cut(s) 411
Hin6I GCGC 1 cut(s) 843
HinP1I GCGC 1 cut(s) 843
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HinfI GANTC 2 cut(s) 802, 1028
HpaII CCGG 1 cut(s) 1019
HphI GGTGA 1 cut(s) 449
Hpy166II GTNNAC 4 cut(s) 88, 172, 714, 1002
Hpy188III TCNNGA 3 cut(s) 75, 865, 1032
Hpy8I GTNNAC 4 cut(s) 88, 172, 714, 1002
HpyAV CCTTC 2 cut(s) 136, 556
HpyCH4III ACNGT 6 cut(s) 85, 109, 169, 892, 985, 1015
HpyCH4IV ACGT 2 cut(s) 79, 998
HpyCH4V TGCA 6 cut(s) 437, 512, 530, 672, 848, 881
HpyF10VI GCNNNNNNNGC 3 cut(s) 391, 434, 913
HpyF3I CTNAG 3 cut(s) 240, 762, 964
HpySE526I ACGT 2 cut(s) 79, 998
Hsp92II CATG 1 cut(s) 411
HspAI GCGC 1 cut(s) 843
Kzo9I GATC 4 cut(s) 213, 443, 522, 789
LmnI GCTCC 1 cut(s) 921
Lsp1109I GCAGC 4 cut(s) 415, 752, 763, 832
LweI GCATC 1 cut(s) 463
MaeI CTAG 1 cut(s) 482
MaeII ACGT 2 cut(s) 79, 998
MaeIII GTNAC 2 cut(s) 217, 992
MalI GATC 4 cut(s) 215, 445, 524, 791
MboI GATC 4 cut(s) 213, 443, 522, 789
MboII GAAGA 7 cut(s) 163, 169, 172, 656, 782, 940, 964
MfeI CAATTG 2 cut(s) 438, 507
MhlI GDGCHC 1 cut(s) 825
MluCI AATT 8 cut(s) 33, 47, 112, 310, 438, 507, 570, 943
MlyI GAGTC 1 cut(s) 796
MmeI TCCRAC 1 cut(s) 383
MroXI GAANNNNTTC 1 cut(s) 571
MseI TTAA 3 cut(s) 44, 297, 831
MspA1I CMGCKG 2 cut(s) 743, 819
MspCI CTTAAG 1 cut(s) 830
MspI CCGG 1 cut(s) 1019
MspR9I CCNGG 2 cut(s) 920, 937
MunI CAATTG 2 cut(s) 438, 507
Mva1269I GAATGC 1 cut(s) 512
MvaI CCWGG 2 cut(s) 920, 937
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 391, 434, 913
NdeI CATATG 1 cut(s) 646
NdeII GATC 4 cut(s) 213, 443, 522, 789
NlaIII CATG 1 cut(s) 411
NlaIV GGNNCC 2 cut(s) 491, 855
NmuCI GTSAC 1 cut(s) 217
NspI RCATGY 1 cut(s) 411
PctI GAATGC 1 cut(s) 512
PdmI GAANNNNTTC 1 cut(s) 571
PfeI GAWTC 1 cut(s) 1028
PflMI CCANNNNNTGG 1 cut(s) 729
PfoI TCCNGGA 1 cut(s) 935
PkrI GCNGC 4 cut(s) 430, 742, 778, 847
PleI GAGTC 1 cut(s) 796
PmaCI CACGTG 1 cut(s) 999
PmlI CACGTG 1 cut(s) 999
PpsI GAGTC 1 cut(s) 796
Ppu21I YACGTR 1 cut(s) 999
PshAI GACNNNNGTC 1 cut(s) 639
Psp6I CCWGG 2 cut(s) 918, 935
PspCI CACGTG 1 cut(s) 999
PspGI CCWGG 2 cut(s) 918, 935
PspN4I GGNNCC 2 cut(s) 491, 855
PspPI GGNCC 2 cut(s) 490, 655
PsrI GAACNNNNNNTAC 2 cut(s) 608, 640
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 4 cut(s) 82, 173, 713, 1003
RsaNI GTAC 4 cut(s) 81, 172, 712, 1002
SaqAI TTAA 3 cut(s) 44, 297, 831
SatI GCNGC 4 cut(s) 429, 741, 777, 846
Sau3AI GATC 4 cut(s) 213, 443, 522, 789
Sau96I GGNCC 2 cut(s) 490, 655
SchI GAGTC 1 cut(s) 796
ScrFI CCNGG 2 cut(s) 920, 937
SduI GDGCHC 1 cut(s) 825
SfaNI GCATC 1 cut(s) 463
SinI GGWCC 2 cut(s) 490, 655
SmlI CTYRAG 1 cut(s) 830
SmoI CTYRAG 1 cut(s) 830
Sse9I AATT 8 cut(s) 33, 47, 112, 310, 438, 507, 570, 943
SsiI CCGC 3 cut(s) 493, 596, 819
SspMI CTAG 1 cut(s) 482
StyD4I CCNGG 2 cut(s) 918, 935
TaaI ACNGT 6 cut(s) 85, 109, 169, 892, 985, 1015
TaiI ACGT 2 cut(s) 82, 1001
TaqI TCGA 2 cut(s) 74, 521
TasI AATT 8 cut(s) 33, 47, 112, 310, 438, 507, 570, 943
TatI WGTACW 1 cut(s) 711
TfiI GAWTC 1 cut(s) 1028
Tru1I TTAA 3 cut(s) 44, 297, 831
Tru9I TTAA 3 cut(s) 44, 297, 831
TscAI CASTG 3 cut(s) 112, 174, 988
TseFI GTSAC 1 cut(s) 217
TseI GCWGC 4 cut(s) 428, 740, 776, 845
Tsp45I GTSAC 1 cut(s) 217
TspDTI ATGAA 2 cut(s) 17, 170
TspGWI ACGGA 1 cut(s) 629
TspRI CASTG 3 cut(s) 112, 174, 988
Van91I CCANNNNNTGG 1 cut(s) 729
Vha464I CTTAAG 1 cut(s) 830
VpaK11BI GGWCC 2 cut(s) 490, 655
XapI RAATTY 3 cut(s) 47, 112, 310
XceI RCATGY 1 cut(s) 411
XcmI CCANNNNNNNNNTGG 1 cut(s) 685
XmnI GAANNNNTTC 1 cut(s) 571
XspI CTAG 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.