pycom08g21000

MACPF domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0007444
Physical Location & Seq
Reverse (-)
20590987 .. 20595014
4028 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1503 bp
ATGTTTGAATTCTCAGGTTGTTGGCAGAAAGATGCAGCCAACACTAAGACCCTTGCTTTTGATGGGGTGTCCATCACTCTCTATTCTGTTGCACTAGAAAAATCTCAGATGGTACTGCGCGAGAATGTTAAAAAAGCAGTCCCATCATCGTGGGACCCTGCTGCATTAGCCAGGTTTATAGAGACCTTTGGAACGCATATTGTTGTTGGTGTGAAGATGGGAGGGAAAGATGTTATATATATGAAGCAGCAGCATTCTTCAACTCTTCAACCGGCCGATATACAGAAAAAGCTAAAGGATATGGCAGATAAAAGGTTTTTAGACGCCAATGGACAGTCTGGTATGCCTTCGGAACAAGGTTACAAGAATGGCAAGGTTCGTAACATGTCTATGTTTGAAATCAGGGAGCAGCGGTTGCGGTTTGCAGATACTAGTCCATCAAGTACTTATTCACACAAGGAGGACATTGTAAGCATTTGCAAGAGGAGAGGTGGAAGTGATACGAGAAATCTATCGCATAATGAATGGTTACAAACTGTTCAGTTGGAGCCTGATGTGATCACAATGTCATTTATACCAATTACCACTCTGTTGAATGGAGTCCCTGGGAGTGGATTCTTGAGCCATGCCATAAACCTTTATTTACGATATAAACCACCAATTGAAGAACTCCATCAGTTTTTGGAGTTTCAGCTGCCGAGGCAATGGGCACCAGTTTTTAGCGACCTTCCCCTTGGTCCACAGCGGAAGCAACAAAGCAATGTATCTCTACAGTTTAGTTTAATGGGGCCCAAACTTTACGTGAACACCAGTCTGGTTGATGTGGGAAAGAGGCCAGTGACTGGCCTGCGACTGTATCTAGAGGGCAAAAGAAGCAACCGTTTGGCCATTCACTTACAACACCTCTCCTCTCTTCCAAAAATCTTCCAACTCGAAGATGATCCACATGGAAACTTTCGTCGTGATCCCTATGATCGTAAATACTATGAGAAAGTCCAGTGGAAGAACTTCTCCCATGTCTGCACTGCCCCTGTGGAGTCTGATGAGGATCATTCAATAGTAACTGGAGCCCAGTTACAGGTTGAGAATTATGGAATAAAGAATATTCTCTTCTTGAGACTCTGCTTCTCCACTGTTTTTGGCGCGACAATTGTAAAGCGTCCTGAGTGGGATGGATCACCAGGTTTGGCACATAAGTCCGGACTCATATCAACACTAATCAGCCATCATTTTACAACAGTTCAGAAGCCACCTCCACGGCCAGCAGATGTGAACATAAACTCTGCCGTTTACCCTGGAGGTCCTCCGGTTCCTATCCAAGCCTCAAAGCTTCGGAAATTCGTTGACACAACAGAGATGGTAAGAGGGCCACAGGAGACTCCAGGTTATTGGGTTGTCTCTGGGGCAAGGCTAGTGGTTGAGAAGGGCAGGATATCACTCCGTGTGAAATATTCTTTATTGACTGTAATTTTACCTGATGAAGAAGAGTTGGAGGGACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MACPF

Protein Analysis

501

Amino Acids

56.34

Weight (kDa)

9.2

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 1 - 214 3.1e-39 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 709
AccB7I CCANNNNNTGG 1 cut(s) 842
AccII CGCG 2 cut(s) 120, 1145
AccIII TCCGGA 1 cut(s) 1199
AciI CCGC 3 cut(s) 412, 418, 745
AclWI GGATC 4 cut(s) 935, 959, 1056, 1183
AcoI YGGCCR 3 cut(s) 273, 885, 1259
AcsI RAATTY 2 cut(s) 8, 1337
AcyI GRCGYC 1 cut(s) 324
AdeI CACNNNGTG 1 cut(s) 1442
AfaI GTAC 2 cut(s) 114, 445
AfiI CCNNNNNNNGG 3 cut(s) 611, 842, 1078
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 7 cut(s) 8, 261, 269, 398, 595, 665, 1056
AhlI ACTAGT 1 cut(s) 431
AjnI CCWGG 5 cut(s) 170, 604, 1180, 1294, 1381
AluBI AGCT 3 cut(s) 292, 694, 1330
AluI AGCT 3 cut(s) 292, 694, 1330
Alw26I GTCTC 4 cut(s) 176, 1111, 1370, 1402
AlwI GGATC 4 cut(s) 935, 959, 1056, 1183
AlwNI CAGNNNCTG 1 cut(s) 842
Aor13HI TCCGGA 1 cut(s) 1199
AoxI GGCC 7 cut(s) 273, 788, 833, 844, 885, 1259, 1367
ApaI GGGCCC 1 cut(s) 792
ApeKI GCWGC 6 cut(s) 35, 161, 247, 250, 409, 694
ApoI RAATTY 2 cut(s) 8, 1337
Asp700I GAANNNNTTC 1 cut(s) 1007
AspLEI GCGC 2 cut(s) 120, 1145
AspS9I GGNCC 6 cut(s) 154, 737, 788, 789, 1301, 1367
AsuHPI GGTGA 1 cut(s) 1170
AvaII GGWCC 3 cut(s) 154, 737, 1301
BaeGI GKGCMC 2 cut(s) 712, 792
BalI TGGCCA 1 cut(s) 887
BanI GGYRCC 1 cut(s) 709
BanII GRGCYC 2 cut(s) 792, 1072
BbvI GCAGC 6 cut(s) 47, 148, 259, 262, 421, 681
BceAI ACGGC 2 cut(s) 1271, 1274
BciT130I CCWGG 5 cut(s) 172, 606, 1182, 1296, 1383
BclI TGATCA 1 cut(s) 558
BcoDI GTCTC 4 cut(s) 176, 1111, 1370, 1402
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 4 cut(s) 95, 432, 860, 1412
BfmI CTRYAG 1 cut(s) 770
BisI GCNGC 6 cut(s) 36, 162, 248, 251, 410, 695
BlsI GCNGC 6 cut(s) 37, 163, 249, 252, 411, 696
BmcAI AGTACT 1 cut(s) 445
Bme1390I CCNGG 5 cut(s) 172, 606, 1182, 1296, 1383
Bme18I GGWCC 3 cut(s) 154, 737, 1301
BmgT120I GGNCC 6 cut(s) 154, 737, 788, 789, 1301, 1367
BmiI GGNNCC 8 cut(s) 155, 156, 549, 711, 789, 790, 1069, 1311
BmrFI CCNGG 5 cut(s) 172, 606, 1182, 1296, 1383
BmrI ACTGGG 1 cut(s) 1066
BmsI GCATC 1 cut(s) 22
BmuI ACTGGG 1 cut(s) 1066
BpmI CTGGAG 3 cut(s) 1086, 1317, 1365
BpuEI CTTGAG 2 cut(s) 640, 1135
BsaAI YACGTR 1 cut(s) 802
BsaBI GATNNNNATC 1 cut(s) 1047
BsaHI GRCGYC 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 6 cut(s) 604, 605, 698, 733, 1256, 1294
BsaWI WCCGGW 2 cut(s) 1199, 1306
BsaXI ACNNNNNCTCC 3 cut(s) 398, 428, 1484
Bsc4I CCNNNNNNNGG 3 cut(s) 611, 842, 1078
Bse118I RCCGGY 1 cut(s) 271
Bse1I ACTGG 7 cut(s) 713, 810, 836, 847, 997, 1069, 1072
Bse3DI GCAATG 2 cut(s) 710, 766
Bse8I GATNNNNATC 1 cut(s) 1047
BseAI TCCGGA 1 cut(s) 1199
BseBI CCWGG 5 cut(s) 172, 606, 1182, 1296, 1383
BseDI CCNNGG 6 cut(s) 604, 605, 698, 733, 1256, 1294
BseGI GGATG 1 cut(s) 1177
BseJI GATNNNNATC 1 cut(s) 1047
BseLI CCNNNNNNNGG 3 cut(s) 611, 842, 1078
BseMI GCAATG 2 cut(s) 710, 766
BseMII CTCAG 3 cut(s) 27, 119, 1155
BseNI ACTGG 7 cut(s) 713, 810, 836, 847, 997, 1069, 1072
BseRI GAGGAG 2 cut(s) 499, 898
BseSI GKGCMC 2 cut(s) 712, 792
BseX3I CGGCCG 1 cut(s) 273
BseXI GCAGC 6 cut(s) 47, 148, 259, 262, 421, 681
BsgI GTGCAG 1 cut(s) 1006
Bsh1236I CGCG 2 cut(s) 120, 1145
Bsh1285I CGRYCG 1 cut(s) 276
BshFI GGCC 7 cut(s) 275, 790, 835, 846, 887, 1261, 1369
BshNI GGYRCC 1 cut(s) 709
BsiEI CGRYCG 1 cut(s) 276
BsiSI CCGG 3 cut(s) 272, 1200, 1307
BslFI GGGAC 3 cut(s) 125, 167, 587
BslI CCNNNNNNNGG 3 cut(s) 611, 842, 1078
BsmAI GTCTC 4 cut(s) 176, 1111, 1370, 1402
BsmFI GGGAC 3 cut(s) 125, 167, 587
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 7 cut(s) 275, 790, 835, 846, 887, 1261, 1369
Bso31I GGTCTC 1 cut(s) 176
Bsp120I GGGCCC 1 cut(s) 788
Bsp1286I GDGCHC 3 cut(s) 712, 792, 1072
Bsp13I TCCGGA 1 cut(s) 1199
Bsp143I GATC 6 cut(s) 558, 940, 964, 973, 1048, 1175
BspACI CCGC 3 cut(s) 412, 418, 745
BspANI GGCC 7 cut(s) 275, 790, 835, 846, 887, 1261, 1369
BspCNI CTCAG 3 cut(s) 26, 118, 1156
BspEI TCCGGA 1 cut(s) 1199
BspFNI CGCG 2 cut(s) 120, 1145
BspLI GGNNCC 8 cut(s) 155, 156, 549, 711, 789, 790, 1069, 1311
BspPI GGATC 4 cut(s) 935, 959, 1056, 1183
BspT107I GGYRCC 1 cut(s) 709
BspTNI GGTCTC 1 cut(s) 176
BsrDI GCAATG 2 cut(s) 710, 766
BsrFI RCCGGY 1 cut(s) 271
BsrI ACTGG 7 cut(s) 713, 810, 836, 847, 997, 1069, 1072
BssAI RCCGGY 1 cut(s) 271
BssECI CCNNGG 6 cut(s) 604, 605, 698, 733, 1256, 1294
BssMI GATC 6 cut(s) 558, 940, 964, 973, 1048, 1175
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 1 cut(s) 733
Bst2UI CCWGG 5 cut(s) 172, 606, 1182, 1296, 1383
Bst4CI ACNGT 9 cut(s) 336, 538, 774, 855, 881, 1135, 1240, 1465, 1500
Bst6I CTCTTC 4 cut(s) 270, 918, 1115, 1479
BstACI GRCGYC 1 cut(s) 324
BstAPI GCANNNNNTGC 1 cut(s) 415
BstBAI YACGTR 1 cut(s) 802
BstC8I GCNNGC 2 cut(s) 848, 1263
BstDEI CTNAG 4 cut(s) 13, 45, 105, 1164
BstDSI CCRYGG 1 cut(s) 1256
BstF5I GGATG 1 cut(s) 1177
BstFNI CGCG 2 cut(s) 120, 1145
BstHHI GCGC 2 cut(s) 120, 1145
BstKTI GATC 6 cut(s) 561, 943, 967, 976, 1051, 1178
BstMAI GTCTC 4 cut(s) 176, 1111, 1370, 1402
BstMBI GATC 6 cut(s) 558, 940, 964, 973, 1048, 1175
BstMCI CGRYCG 1 cut(s) 276
BstMWI GCNNNNNNNGC 4 cut(s) 167, 415, 700, 873
BstNI CCWGG 5 cut(s) 172, 606, 1182, 1296, 1383
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 5 cut(s) 170, 604, 1180, 1294, 1381
BstSFI CTRYAG 1 cut(s) 770
BstSLI GKGCMC 2 cut(s) 712, 792
BstUI CGCG 2 cut(s) 120, 1145
BstV1I GCAGC 6 cut(s) 47, 148, 259, 262, 421, 681
BstXI CCANNNNNNTGG 2 cut(s) 150, 1389
BstZI CGGCCG 1 cut(s) 273
BsuRI GGCC 7 cut(s) 275, 790, 835, 846, 887, 1261, 1369
BtgI CCRYGG 1 cut(s) 1256
BtsCI GGATG 1 cut(s) 1177
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 4 cut(s) 843, 1004, 1023, 1131
Cac8I GCNNGC 2 cut(s) 848, 1263
CaiI CAGNNNCTG 1 cut(s) 842
CfoI GCGC 2 cut(s) 120, 1145
Cfr10I RCCGGY 1 cut(s) 271
Cfr13I GGNCC 6 cut(s) 154, 737, 788, 789, 1301, 1367
CseI GACGC 2 cut(s) 332, 1148
CsiI ACCWGGT 1 cut(s) 1180
Csp6I GTAC 2 cut(s) 113, 444
CspCI CAANNNNNGTGG 4 cut(s) 1120, 1155, 1395, 1430
CviAII CATG 4 cut(s) 385, 626, 947, 1016
CviQI GTAC 2 cut(s) 113, 444
DdeI CTNAG 4 cut(s) 13, 45, 105, 1164
DpnI GATC 6 cut(s) 560, 942, 966, 975, 1050, 1177
DpnII GATC 6 cut(s) 558, 940, 964, 973, 1048, 1175
DraIII CACNNNGTG 1 cut(s) 1442
EaeI YGGCCR 3 cut(s) 273, 885, 1259
EagI CGGCCG 1 cut(s) 273
Eam1104I CTCTTC 4 cut(s) 270, 918, 1115, 1479
EarI CTCTTC 4 cut(s) 270, 918, 1115, 1479
EclXI CGGCCG 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 733
Eco24I GRGCYC 2 cut(s) 792, 1072
Eco31I GGTCTC 1 cut(s) 176
Eco32I GATATC 1 cut(s) 1434
Eco47I GGWCC 3 cut(s) 154, 737, 1301
Eco52I CGGCCG 1 cut(s) 273
EcoO109I RGGNCCY 3 cut(s) 154, 788, 1301
EcoRI GAATTC 1 cut(s) 8
EcoRII CCWGG 5 cut(s) 170, 604, 1180, 1294, 1381
EcoRV GATATC 1 cut(s) 1434
EcoT14I CCWWGG 1 cut(s) 733
EcoT38I GRGCYC 2 cut(s) 792, 1072
ErhI CCWWGG 1 cut(s) 733
FaeI CATG 4 cut(s) 388, 629, 950, 1019
FaqI GGGAC 3 cut(s) 125, 167, 587
FatI CATG 4 cut(s) 384, 625, 946, 1015
FbaI TGATCA 1 cut(s) 558
Fnu4HI GCNGC 6 cut(s) 36, 162, 248, 251, 410, 695
FokI GGATG 1 cut(s) 1184
FriOI GRGCYC 2 cut(s) 792, 1072
Fsp4HI GCNGC 6 cut(s) 36, 162, 248, 251, 410, 695
FspBI CTAG 4 cut(s) 95, 432, 860, 1412
GlaI GCGC 2 cut(s) 119, 1144
GluI GCNGC 6 cut(s) 36, 162, 248, 251, 410, 695
GsuI CTGGAG 3 cut(s) 1086, 1317, 1365
HaeIII GGCC 7 cut(s) 275, 790, 835, 846, 887, 1261, 1369
HapII CCGG 3 cut(s) 272, 1200, 1307
HgaI GACGC 2 cut(s) 332, 1148
HhaI GCGC 2 cut(s) 120, 1145
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 4 cut(s) 388, 629, 950, 1019
Hin6I GCGC 2 cut(s) 118, 1143
HinP1I GCGC 2 cut(s) 118, 1143
HincII GTYRAC 1 cut(s) 1345
HindII GTYRAC 1 cut(s) 1345
HindIII AAGCTT 1 cut(s) 1328
HinfI GANTC 6 cut(s) 600, 615, 1037, 1119, 1203, 1378
HpaII CCGG 3 cut(s) 272, 1200, 1307
HphI GGTGA 1 cut(s) 1170
Hpy166II GTNNAC 5 cut(s) 740, 805, 1273, 1291, 1345
Hpy188I TCNGA 5 cut(s) 108, 352, 1042, 1245, 1335
Hpy188III TCNNGA 6 cut(s) 619, 860, 962, 1114, 1163, 1200
Hpy8I GTNNAC 5 cut(s) 740, 805, 1273, 1291, 1345
Hpy99I CGWCG 1 cut(s) 963
HpyAV CCTTC 3 cut(s) 357, 737, 1417
HpyCH4III ACNGT 9 cut(s) 336, 538, 774, 855, 881, 1135, 1240, 1465, 1500
HpyCH4IV ACGT 1 cut(s) 801
HpyCH4V TGCA 6 cut(s) 35, 92, 164, 425, 480, 1023
HpyF10VI GCNNNNNNNGC 4 cut(s) 167, 415, 700, 873
HpyF3I CTNAG 4 cut(s) 13, 45, 105, 1164
HpySE526I ACGT 1 cut(s) 801
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 4 cut(s) 388, 629, 950, 1019
HspAI GCGC 2 cut(s) 118, 1143
KflI GGGWCCC 1 cut(s) 154
Kpn2I TCCGGA 1 cut(s) 1199
Ksp22I TGATCA 1 cut(s) 558
Kzo9I GATC 6 cut(s) 558, 940, 964, 973, 1048, 1175
LmnI GCTCC 3 cut(s) 406, 547, 1067
Lsp1109I GCAGC 6 cut(s) 47, 148, 259, 262, 421, 681
LweI GCATC 1 cut(s) 22
MabI ACCWGGT 1 cut(s) 1180
MaeI CTAG 4 cut(s) 95, 432, 860, 1412
MaeII ACGT 1 cut(s) 801
MaeIII GTNAC 6 cut(s) 359, 380, 528, 838, 1060, 1074
MalI GATC 6 cut(s) 560, 942, 966, 975, 1050, 1177
MboI GATC 6 cut(s) 558, 940, 964, 973, 1048, 1175
MfeI CAATTG 2 cut(s) 660, 1149
MhlI GDGCHC 3 cut(s) 712, 792, 1072
MlsI TGGCCA 1 cut(s) 887
MluCI AATT 7 cut(s) 8, 579, 660, 1087, 1149, 1337, 1467
MluNI TGGCCA 1 cut(s) 887
MlyI GAGTC 5 cut(s) 609, 1046, 1113, 1197, 1372
MmeI TCCRAC 3 cut(s) 525, 952, 1470
Mox20I TGGCCA 1 cut(s) 887
MroI TCCGGA 1 cut(s) 1199
MroXI GAANNNNTTC 1 cut(s) 1007
MscI TGGCCA 1 cut(s) 887
MseI TTAA 2 cut(s) 129, 782
MslI CAYNNNNRTG 2 cut(s) 148, 389
Msp20I TGGCCA 1 cut(s) 887
MspA1I CMGCKG 3 cut(s) 412, 694, 745
MspI CCGG 3 cut(s) 272, 1200, 1307
MspR9I CCNGG 5 cut(s) 172, 606, 1182, 1296, 1383
MunI CAATTG 2 cut(s) 660, 1149
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 5 cut(s) 172, 606, 1182, 1296, 1383
MvnI CGCG 2 cut(s) 120, 1145
MwoI GCNNNNNNNGC 4 cut(s) 167, 415, 700, 873
NdeII GATC 6 cut(s) 558, 940, 964, 973, 1048, 1175
NlaIII CATG 4 cut(s) 388, 629, 950, 1019
NlaIV GGNNCC 8 cut(s) 155, 156, 549, 711, 789, 790, 1069, 1311
NmeAIII GCCGAG 1 cut(s) 723
NmuCI GTSAC 1 cut(s) 838
NspI RCATGY 1 cut(s) 388
PasI CCCWGGG 1 cut(s) 605
PciI ACATGT 1 cut(s) 384
PctI GAATGC 1 cut(s) 253
PdmI GAANNNNTTC 1 cut(s) 1007
PfeI GAWTC 1 cut(s) 615
PflMI CCANNNNNTGG 1 cut(s) 842
PkrI GCNGC 6 cut(s) 37, 163, 249, 252, 411, 696
PleI GAGTC 5 cut(s) 608, 1045, 1113, 1197, 1372
PpsI GAGTC 5 cut(s) 608, 1045, 1113, 1197, 1372
Ppu21I YACGTR 1 cut(s) 802
PpuMI RGGWCCY 2 cut(s) 154, 1301
PscI ACATGT 1 cut(s) 384
Psp5II RGGWCCY 2 cut(s) 154, 1301
Psp6I CCWGG 5 cut(s) 170, 604, 1180, 1294, 1381
PspGI CCWGG 5 cut(s) 170, 604, 1180, 1294, 1381
PspN4I GGNNCC 8 cut(s) 155, 156, 549, 711, 789, 790, 1069, 1311
PspOMI GGGCCC 1 cut(s) 788
PspPI GGNCC 6 cut(s) 154, 737, 788, 789, 1301, 1367
PspPPI RGGWCCY 2 cut(s) 154, 1301
PstNI CAGNNNCTG 1 cut(s) 842
PvuII CAGCTG 1 cut(s) 694
RsaI GTAC 2 cut(s) 114, 445
RsaNI GTAC 2 cut(s) 113, 444
RseI CAYNNNNRTG 2 cut(s) 148, 389
SaqAI TTAA 2 cut(s) 129, 782
SatI GCNGC 6 cut(s) 36, 162, 248, 251, 410, 695
Sau3AI GATC 6 cut(s) 558, 940, 964, 973, 1048, 1175
Sau96I GGNCC 6 cut(s) 154, 737, 788, 789, 1301, 1367
ScaI AGTACT 1 cut(s) 445
SchI GAGTC 5 cut(s) 609, 1046, 1113, 1197, 1372
ScrFI CCNGG 5 cut(s) 172, 606, 1182, 1296, 1383
SduI GDGCHC 3 cut(s) 712, 792, 1072
SexAI ACCWGGT 1 cut(s) 1180
SfaNI GCATC 1 cut(s) 22
SfcI CTRYAG 1 cut(s) 770
SinI GGWCC 3 cut(s) 154, 737, 1301
SmiMI CAYNNNNRTG 2 cut(s) 148, 389
SmlI CTYRAG 2 cut(s) 619, 1114
SmoI CTYRAG 2 cut(s) 619, 1114
SpeI ACTAGT 1 cut(s) 431
Sse9I AATT 7 cut(s) 8, 579, 660, 1087, 1149, 1337, 1467
SsiI CCGC 3 cut(s) 412, 418, 745
SspI AATATT 2 cut(s) 1105, 1451
SspMI CTAG 4 cut(s) 95, 432, 860, 1412
StyD4I CCNGG 5 cut(s) 170, 604, 1180, 1294, 1381
StyI CCWWGG 1 cut(s) 733
TaaI ACNGT 9 cut(s) 336, 538, 774, 855, 881, 1135, 1240, 1465, 1500
TaiI ACGT 1 cut(s) 804
TaqI TCGA 1 cut(s) 933
TasI AATT 7 cut(s) 8, 579, 660, 1087, 1149, 1337, 1467
TatI WGTACW 1 cut(s) 443
TfiI GAWTC 1 cut(s) 615
Tru1I TTAA 2 cut(s) 129, 782
Tru9I TTAA 2 cut(s) 129, 782
TscAI CASTG 4 cut(s) 843, 1004, 1030, 1138
TseFI GTSAC 1 cut(s) 838
TseI GCWGC 6 cut(s) 35, 161, 247, 250, 409, 694
Tsp45I GTSAC 1 cut(s) 838
TspDTI ATGAA 3 cut(s) 257, 537, 1494
TspGWI ACGGA 1 cut(s) 1430
TspRI CASTG 4 cut(s) 843, 1004, 1030, 1138
Van91I CCANNNNNTGG 1 cut(s) 842
VpaK11BI GGWCC 3 cut(s) 154, 737, 1301
XapI RAATTY 2 cut(s) 8, 1337
XbaI TCTAGA 1 cut(s) 859
XceI RCATGY 1 cut(s) 388
XmnI GAANNNNTTC 1 cut(s) 1007
XspI CTAG 4 cut(s) 95, 432, 860, 1412
ZrmI AGTACT 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.