Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0012169

Physical Location & Seq

Genomic Coordinates Reverse (-)

2566023 .. 2569440

Gene Structure 3418 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g03470.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1464 bp

ATGGCTTTCATGATGAGCACAACCCCCGGCATCCCACCGTCACCGTCACCACCAGCGCTCTCTCAACCACAACCACAACCACCAGCACCTCTGCCAGTCCGTACAATCCCCGGAACGTACGGCTGGCCGCTCCTGGGCCCCATCTCGGACCGCCTGGACTACTTCTGGTTCCAGGGTCCCGAAACATTCTTCAAGAAGCGCACCGAGAAGTACAAGAGCACGGTGTTCCGCACAAATGTCCCTCCCAGCTTCCCGCTATTCGTCAACGTCAACCCCAACGTCATCGCCGTCCTCGACTGCAAGTCCTTCTCGTACATGTTCGACATGGAGTTGGTCGAGAAGAAGAATGTTCTTGTCGGCGACTTCATGCCGAGTGTTAAGTTCACCGGCGACTTGAGGACCTGCGCTTATCTTGATACATCCGAACCCCAACACTCCCAGATCAAAAACTTTGCCATGGACATCTTGAAAAGAAGCTCCAAGACATGGGTGCCTGAGCTCACTGCCAACCTCTCAACCTTCTGGGACACAATAGAAGCAGACGTCTCCAAAGATGGGAGTGCCAGCTATTTGATCCCACTTCAGAAGTTCATCTTCAAGTTTCTCACTAAATGCTTAGTTGGAGCTGACCTGGCAAGCTCGCCCAAGATAGCGAAGTCCGGCTACGCCATGCTTGACCGGTGGCTCGCCCTCCAGCTCCTCCCCACAGTCAAGATTGGTGTGCTTCAGCCCCTGGAGGAGATTCTCCTCCATTCTTTTGCATACCCTTCTTTTCTAGTCAGTGGAGACTACAACCAGCTTTACCAGTTTATAGAAGAACATGGCAAAGAAGTAGTAAACAGAGGTGAAACCGATTTCGGACTGACCAAAGAAGTAACAATTCACAACCTTCTCTTTGTACTTGGTTTCAATGCATTTGGTGGGTTTTCTGTCTTCCTGCCAAGTTTGATAGGCACAATAGCAAGCGACACCACCGGTTTACAGGCCAAAATAGTCAAGGAAGTCAGAGAAAACGCCGGTTCAACTTTGAGTTTCGACTCGGTGAAAAACTTGCAACTGGTTCAGTCCGTAGTGTACGAAGCCCTCCGGTTGAACCCACCGGTTCCTCTCCAATTTGCCCGAGCCAGGAAGGACTTCCAACTCAGTTCACACGACTCGGCCTTTAACATCAAGAAGGGTGAGTTGCTCTGTGGGTTTCAACAACAGGTCATGAGGGATGAGAAAGTATTCGATGAACCGGACACCTTTAAACCGGACCGGTTCATGAAGAACAAAGAGTTGCTGAACTACTTGTACTGGTCCAACGGACCGCAGACCGGTTCACCAAGTGAGTCCAACAAGCAGTGCGCTGCAAAGGACTATGTGACCCTCACTGCCACTTTGCTTGTGGCATATGTATTTCAAAGATATGATTCAATCACAGGAACCGGTACTTCAATCACAGCCCTCGAGAAGGCCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006950 GO:0008150 GO:0008152 GO:0008202 GO:0009507 GO:0009526 GO:0009536 GO:0009611 GO:0009941 GO:0009987 GO:0016125 GO:0016491 GO:0019752 GO:0031967 GO:0031975 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0055114 GO:0071704 GO:1901360 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00592 ko01110 map00592 map01110

Pfam Domains

Protein Families

p450

Protein Analysis

488

Amino Acids

54.49

Weight (kDa)

7.57

Isoelectric Point (pI)

43.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	327 - 426	3.1e-16	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15440
fragaria_vesca	FvH4_6g42740
malus_domestica	MD09G1071400.v1.1 MD17G1100800.v1.1
prunus_persica	Prupe.3G213800_v2.0.a1
pyrus_communis	pycom09g03470 pycom17g09550
rosa_chinensis	RchiOBHm_Chr2g0158631
rosa_laevigata	RLG00000021101
rosa_multiflora	Rmu_co8067608.1_g000001 Rmu_sc0004188.1_g000015 Rmu_sc0034116.1_g000001
rosa_roxburghii	Rroxscaffold_2G00091160
rosa_rugosa	Rorug02G0471000
rosa_samantha	Rh2AG536700 Rh2BG549900 Rh2CG520400 Rh2DG559500
rosa_wichuraiana	Rw2G044430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	546
Acc36I	ACCTGC	1 cut(s)	410
AccB1I	GGYRCC	1 cut(s)	490
AccB7I	CCANNNNNTGG	1 cut(s)	486
AccBSI	CCGCTC	1 cut(s)	130
AciI	CCGC	5 cut(s)	128, 151, 229, 254, 1310
AclWI	GGATC	1 cut(s)	568
AcoI	YGGCCR	1 cut(s)	125
AcuI	CTGAAG	2 cut(s)	566, 710
AcyI	GRCGYC	1 cut(s)	543
AdeI	CACNNNGTG	1 cut(s)	1328
AfaI	GTAC	8 cut(s)	103, 119, 212, 314, 900, 1076, 1295, 1432
AfeI	AGCGCT	1 cut(s)	57
AfiI	CCNNNNNNNGG	7 cut(s)	134, 145, 486, 555, 1125, 1316, 1453
AflIII	ACRYGT	1 cut(s)	315
AgeI	ACCGGT	6 cut(s)	678, 974, 1099, 1257, 1316, 1427
AhdI	GACNNNNNGTC	1 cut(s)	301
AjnI	CCWGG	6 cut(s)	132, 153, 171, 630, 732, 1124
AluBI	AGCT	8 cut(s)	249, 477, 499, 567, 626, 639, 697, 799
AluI	AGCT	8 cut(s)	249, 477, 499, 567, 626, 639, 697, 799
Alw21I	GWGCWC	3 cut(s)	20, 221, 501
Alw26I	GTCTC	2 cut(s)	550, 780
AlwI	GGATC	1 cut(s)	568
AlwNI	CAGNNNCTG	1 cut(s)	733
Ama87I	CYCGRG	2 cut(s)	1119, 1448
Aor51HI	AGCGCT	1 cut(s)	57
AoxI	GGCC	5 cut(s)	125, 136, 984, 1158, 1455
ApaI	GGGCCC	1 cut(s)	140
ApeKI	GCWGC	1 cut(s)	1349
AsiGI	ACCGGT	6 cut(s)	678, 974, 1099, 1257, 1316, 1427
Asp700I	GAANNNNTTC	2 cut(s)	1133, 1226
AspLEI	GCGC	4 cut(s)	58, 201, 407, 1349
AspS9I	GGNCC	8 cut(s)	136, 137, 148, 176, 399, 1255, 1299, 1307
AsuC2I	CCSGG	2 cut(s)	27, 111
AsuHPI	GGTGA	7 cut(s)	33, 39, 376, 857, 1054, 1190, 1314
AvaI	CYCGRG	2 cut(s)	1119, 1448
AvaII	GGWCC	6 cut(s)	148, 176, 399, 1255, 1299, 1307
BaeGI	GKGCMC	1 cut(s)	140
BanI	GGYRCC	1 cut(s)	490
BanII	GRGCYC	2 cut(s)	140, 501
BarI	GAAGNNNNNNTAC	2 cut(s)	647, 679
BbsI	GAAGAC	1 cut(s)	925
Bbv12I	GWGCWC	3 cut(s)	20, 221, 501
BbvI	GCAGC	1 cut(s)	1336
BccI	CCATC	2 cut(s)	149, 548
BceAI	ACGGC	2 cut(s)	136, 272
BciT130I	CCWGG	6 cut(s)	134, 155, 173, 632, 734, 1126
BcnI	CCSGG	2 cut(s)	27, 111
BcoDI	GTCTC	2 cut(s)	550, 780
BfaI	CTAG	1 cut(s)	776
BfoI	RGCGCY	1 cut(s)	59
BfuAI	ACCTGC	1 cut(s)	410
BisI	GCNGC	2 cut(s)	128, 1350
BlsI	GCNGC	2 cut(s)	129, 1351
Bme1390I	CCNGG	8 cut(s)	27, 111, 134, 155, 173, 632, 734, 1126
Bme18I	GGWCC	6 cut(s)	148, 176, 399, 1255, 1299, 1307
BmeRI	GACNNNNNGTC	1 cut(s)	301
BmeT110I	CYCGRG	2 cut(s)	1119, 1448
BmgT120I	GGNCC	8 cut(s)	136, 137, 148, 176, 399, 1255, 1299, 1307
BmiI	GGNNCC	8 cut(s)	138, 139, 170, 177, 178, 492, 1104, 1426
BmrFI	CCNGG	8 cut(s)	27, 111, 134, 155, 173, 632, 734, 1126
BmsI	GCATC	1 cut(s)	39
BpiI	GAAGAC	1 cut(s)	925
BpmI	CTGGAG	2 cut(s)	677, 755
Bpu10I	CCTNAGC	1 cut(s)	495
BpuEI	CTTGAG	1 cut(s)	415
BpuMI	CCSGG	2 cut(s)	27, 111
BsaHI	GRCGYC	1 cut(s)	543
BsaJI	CCNNGG	6 cut(s)	25, 109, 133, 172, 456, 732
BsaWI	WCCGGW	9 cut(s)	678, 974, 1086, 1099, 1237, 1252, 1257, 1316, 1427
Bsc4I	CCNNNNNNNGG	7 cut(s)	134, 145, 486, 555, 1125, 1316, 1453
Bse118I	RCCGGY	8 cut(s)	386, 678, 974, 1016, 1099, 1257, 1316, 1427
Bse1I	ACTGG	4 cut(s)	95, 805, 1062, 1301
BseBI	CCWGG	6 cut(s)	134, 155, 173, 632, 734, 1126
BseDI	CCNNGG	6 cut(s)	25, 109, 133, 172, 456, 732
BseGI	GGATG	3 cut(s)	30, 419, 1222
BseLI	CCNNNNNNNGG	7 cut(s)	134, 145, 486, 555, 1125, 1316, 1453
BseMII	CTCAG	2 cut(s)	486, 1156
BseNI	ACTGG	4 cut(s)	95, 805, 1062, 1301
BseRI	GAGGAG	3 cut(s)	689, 737, 752
BseSI	GKGCMC	1 cut(s)	140
BseXI	GCAGC	1 cut(s)	1336
BseYI	CCCAGC	1 cut(s)	245
BshFI	GGCC	5 cut(s)	127, 138, 986, 1160, 1457
BshNI	GGYRCC	1 cut(s)	490
BshTI	ACCGGT	6 cut(s)	678, 974, 1099, 1257, 1316, 1427
BsiHKAI	GWGCWC	3 cut(s)	20, 221, 501
BsiHKCI	CYCGRG	2 cut(s)	1119, 1448
BsiWI	CGTACG	1 cut(s)	117
BslFI	GGGAC	3 cut(s)	162, 224, 539
BslI	CCNNNNNNNGG	7 cut(s)	134, 145, 486, 555, 1125, 1316, 1453
BsmAI	GTCTC	2 cut(s)	550, 780
BsmBI	CGTCTC	1 cut(s)	550
BsmFI	GGGAC	3 cut(s)	162, 224, 539
BsnI	GGCC	5 cut(s)	127, 138, 986, 1160, 1457
BsoBI	CYCGRG	2 cut(s)	1119, 1448
Bsp120I	GGGCCC	1 cut(s)	136
Bsp1286I	GDGCHC	4 cut(s)	20, 140, 221, 501
Bsp143I	GATC	2 cut(s)	441, 573
Bsp19I	CCATGG	1 cut(s)	456
BspACI	CCGC	5 cut(s)	128, 151, 229, 254, 1310
BspANI	GGCC	5 cut(s)	127, 138, 986, 1160, 1457
BspCNI	CTCAG	2 cut(s)	487, 1155
BspHI	TCATGA	3 cut(s)	9, 1209, 1263
BspLI	GGNNCC	8 cut(s)	138, 139, 170, 177, 178, 492, 1104, 1426
BspMI	ACCTGC	1 cut(s)	410
BspPI	GGATC	1 cut(s)	568
BspT107I	GGYRCC	1 cut(s)	490
BsrBI	CCGCTC	1 cut(s)	130
BsrFI	RCCGGY	8 cut(s)	386, 678, 974, 1016, 1099, 1257, 1316, 1427
BsrI	ACTGG	4 cut(s)	95, 805, 1062, 1301
BssAI	RCCGGY	8 cut(s)	386, 678, 974, 1016, 1099, 1257, 1316, 1427
BssECI	CCNNGG	6 cut(s)	25, 109, 133, 172, 456, 732
BssMI	GATC	2 cut(s)	441, 573
BssNI	GRCGYC	1 cut(s)	543
BssT1I	CCWWGG	1 cut(s)	456
Bst2UI	CCWGG	6 cut(s)	134, 155, 173, 632, 734, 1126
Bst4CI	ACNGT	4 cut(s)	39, 45, 223, 709
BstACI	GRCGYC	1 cut(s)	543
BstC8I	GCNNGC	6 cut(s)	125, 565, 637, 641, 687, 964
BstDEI	CTNAG	3 cut(s)	495, 616, 1142
BstDSI	CCRYGG	1 cut(s)	456
BstENI	CCTNNNNNAGG	1 cut(s)	1451
BstF5I	GGATG	3 cut(s)	30, 419, 1222
BstH2I	RGCGCY	1 cut(s)	59
BstHHI	GCGC	4 cut(s)	58, 201, 407, 1349
BstKTI	GATC	2 cut(s)	444, 576
BstMAI	GTCTC	2 cut(s)	550, 780
BstMBI	GATC	2 cut(s)	441, 573
BstMWI	GCNNNNNNNGC	1 cut(s)	632
BstNI	CCWGG	6 cut(s)	134, 155, 173, 632, 734, 1126
BstNSI	RCATGY	1 cut(s)	319
BstSCI	CCNGG	8 cut(s)	25, 109, 132, 153, 171, 630, 732, 1124
BstSLI	GKGCMC	1 cut(s)	140
BstV1I	GCAGC	1 cut(s)	1336
BstV2I	GAAGAC	1 cut(s)	925
BsuRI	GGCC	5 cut(s)	127, 138, 986, 1160, 1457
BtgI	CCRYGG	1 cut(s)	456
BtgZI	GCGATG	1 cut(s)	268
BtsCI	GGATG	3 cut(s)	30, 419, 1222
BtsI	GCAGTG	3 cut(s)	501, 1349, 1371
BtsIMutI	CAGTG	4 cut(s)	501, 787, 1349, 1371
BveI	ACCTGC	1 cut(s)	410
Cac8I	GCNNGC	6 cut(s)	125, 565, 637, 641, 687, 964
CaiI	CAGNNNCTG	1 cut(s)	733
CciI	TCATGA	3 cut(s)	9, 1209, 1263
CfoI	GCGC	4 cut(s)	58, 201, 407, 1349
Cfr10I	RCCGGY	8 cut(s)	386, 678, 974, 1016, 1099, 1257, 1316, 1427
Cfr13I	GGNCC	8 cut(s)	136, 137, 148, 176, 399, 1255, 1299, 1307
CpoI	CGGWCCG	3 cut(s)	148, 1255, 1307
Csp6I	GTAC	8 cut(s)	102, 118, 211, 313, 899, 1075, 1294, 1431
CspAI	ACCGGT	6 cut(s)	678, 974, 1099, 1257, 1316, 1427
CspCI	CAANNNNNGTGG	2 cut(s)	1366, 1401
CspI	CGGWCCG	3 cut(s)	148, 1255, 1307
CviQI	GTAC	8 cut(s)	102, 118, 211, 313, 899, 1075, 1294, 1431
DdeI	CTNAG	3 cut(s)	495, 616, 1142
DpnI	GATC	2 cut(s)	443, 575
DpnII	GATC	2 cut(s)	441, 573
DraI	TTTAAA	1 cut(s)	1249
DraIII	CACNNNGTG	1 cut(s)	1328
DriI	GACNNNNNGTC	1 cut(s)	301
EaeI	YGGCCR	1 cut(s)	125
Eam1105I	GACNNNNNGTC	1 cut(s)	301
Ecl136II	GAGCTC	1 cut(s)	499
Eco130I	CCWWGG	1 cut(s)	456
Eco24I	GRGCYC	2 cut(s)	140, 501
Eco47I	GGWCC	6 cut(s)	148, 176, 399, 1255, 1299, 1307
Eco47III	AGCGCT	1 cut(s)	57
Eco53kI	GAGCTC	1 cut(s)	499
Eco57I	CTGAAG	2 cut(s)	566, 710
Eco88I	CYCGRG	2 cut(s)	1119, 1448
EcoICRI	GAGCTC	1 cut(s)	499
EcoNI	CCTNNNNNAGG	1 cut(s)	1451
EcoO109I	RGGNCCY	3 cut(s)	137, 176, 399
EcoRII	CCWGG	6 cut(s)	132, 153, 171, 630, 732, 1124
EcoT14I	CCWWGG	1 cut(s)	456
EcoT22I	ATGCAT	1 cut(s)	916
EcoT38I	GRGCYC	2 cut(s)	140, 501
ErhI	CCWWGG	1 cut(s)	456
Esp3I	CGTCTC	1 cut(s)	550
FalI	AAGNNNNNCTT	2 cut(s)	578, 610
FaqI	GGGAC	3 cut(s)	162, 224, 539
FauI	CCCGC	1 cut(s)	261
FauNDI	CATATG	1 cut(s)	1393
Fnu4HI	GCNGC	2 cut(s)	128, 1350
FokI	GGATG	3 cut(s)	17, 406, 1229
FriOI	GRGCYC	2 cut(s)	140, 501
Fsp4HI	GCNGC	2 cut(s)	128, 1350
FspBI	CTAG	1 cut(s)	776
GlaI	GCGC	4 cut(s)	57, 200, 406, 1348
GluI	GCNGC	2 cut(s)	128, 1350
GsaI	CCCAGC	1 cut(s)	249
GsuI	CTGGAG	2 cut(s)	677, 755
HaeII	RGCGCY	1 cut(s)	59
HaeIII	GGCC	5 cut(s)	127, 138, 986, 1160, 1457
HhaI	GCGC	4 cut(s)	58, 201, 407, 1349
Hin1I	GRCGYC	1 cut(s)	543
Hin6I	GCGC	4 cut(s)	56, 199, 405, 1347
HinP1I	GCGC	4 cut(s)	56, 199, 405, 1347
HincII	GTYRAC	2 cut(s)	265, 271
HindII	GTYRAC	2 cut(s)	265, 271
HinfI	GANTC	5 cut(s)	742, 1037, 1154, 1331, 1412
HphI	GGTGA	7 cut(s)	33, 39, 376, 857, 1054, 1190, 1314
Hpy166II	GTNNAC	8 cut(s)	265, 271, 384, 838, 980, 1075, 1148, 1322
Hpy188I	TCNGA	5 cut(s)	148, 424, 585, 860, 1007
Hpy8I	GTNNAC	8 cut(s)	265, 271, 384, 838, 980, 1075, 1148, 1322
HpyAV	CCTTC	7 cut(s)	316, 529, 777, 899, 1123, 1168, 1447
HpyCH4III	ACNGT	4 cut(s)	39, 45, 223, 709
HpyCH4IV	ACGT	4 cut(s)	116, 267, 279, 543
HpyCH4V	TGCA	5 cut(s)	300, 761, 914, 1054, 1352
HpyF10VI	GCNNNNNNNGC	1 cut(s)	632
HpyF3I	CTNAG	3 cut(s)	495, 616, 1142
HpySE526I	ACGT	4 cut(s)	116, 267, 279, 543
Hsp92I	GRCGYC	1 cut(s)	543
HspAI	GCGC	4 cut(s)	56, 199, 405, 1347
KflI	GGGWCCC	1 cut(s)	176
Kzo9I	GATC	2 cut(s)	441, 573
LmnI	GCTCC	4 cut(s)	135, 482, 623, 702
Lsp1109I	GCAGC	1 cut(s)	1336
LweI	GCATC	1 cut(s)	39
MaeI	CTAG	1 cut(s)	776
MaeII	ACGT	4 cut(s)	116, 267, 279, 543
MaeIII	GTNAC	4 cut(s)	39, 45, 874, 1363
MalI	GATC	2 cut(s)	443, 575
MbiI	CCGCTC	1 cut(s)	130
MboI	GATC	2 cut(s)	441, 573
MboII	GAAGA	7 cut(s)	181, 352, 355, 586, 827, 925, 1279
MhlI	GDGCHC	4 cut(s)	20, 140, 221, 501
MluCI	AATT	2 cut(s)	879, 1112
MlyI	GAGTC	3 cut(s)	1031, 1148, 1340
MmeI	TCCRAC	4 cut(s)	601, 1162, 1326, 1359
Mph1103I	ATGCAT	1 cut(s)	916
MroXI	GAANNNNTTC	2 cut(s)	1133, 1226
MseI	TTAA	3 cut(s)	378, 1164, 1248
MspR9I	CCNGG	8 cut(s)	27, 111, 134, 155, 173, 632, 734, 1126
MvaI	CCWGG	6 cut(s)	134, 155, 173, 632, 734, 1126
MwoI	GCNNNNNNNGC	1 cut(s)	632
NciI	CCSGG	2 cut(s)	27, 111
NcoI	CCATGG	1 cut(s)	456
NdeI	CATATG	1 cut(s)	1393
NdeII	GATC	2 cut(s)	441, 573
NlaIV	GGNNCC	8 cut(s)	138, 139, 170, 177, 178, 492, 1104, 1426
NmeAIII	GCCGAG	2 cut(s)	396, 1136
NmuCI	GTSAC	3 cut(s)	39, 45, 1363
NsiI	ATGCAT	1 cut(s)	916
NspI	RCATGY	1 cut(s)	319
PaeR7I	CTCGAG	1 cut(s)	1448
PagI	TCATGA	3 cut(s)	9, 1209, 1263
PciI	ACATGT	1 cut(s)	315
PcsI	WCGNNNNNNNCGW	2 cut(s)	285, 291
PdmI	GAANNNNTTC	2 cut(s)	1133, 1226
PfeI	GAWTC	2 cut(s)	742, 1412
Pfl23II	CGTACG	1 cut(s)	117
PflMI	CCANNNNNTGG	1 cut(s)	486
PinAI	ACCGGT	6 cut(s)	678, 974, 1099, 1257, 1316, 1427
PkrI	GCNGC	2 cut(s)	129, 1351
PleI	GAGTC	3 cut(s)	1031, 1148, 1339
PpsI	GAGTC	3 cut(s)	1031, 1148, 1339
PpuMI	RGGWCCY	2 cut(s)	176, 399
PscI	ACATGT	1 cut(s)	315
Psp124BI	GAGCTC	1 cut(s)	501
Psp5II	RGGWCCY	2 cut(s)	176, 399
Psp6I	CCWGG	6 cut(s)	132, 153, 171, 630, 732, 1124
PspFI	CCCAGC	1 cut(s)	245
PspGI	CCWGG	6 cut(s)	132, 153, 171, 630, 732, 1124
PspLI	CGTACG	1 cut(s)	117
PspN4I	GGNNCC	8 cut(s)	138, 139, 170, 177, 178, 492, 1104, 1426
PspOMI	GGGCCC	1 cut(s)	136
PspPI	GGNCC	8 cut(s)	136, 137, 148, 176, 399, 1255, 1299, 1307
PspPPI	RGGWCCY	2 cut(s)	176, 399
PsrI	GAACNNNNNNTAC	2 cut(s)	1277, 1309
PstNI	CAGNNNCTG	1 cut(s)	733
RsaI	GTAC	8 cut(s)	103, 119, 212, 314, 900, 1076, 1295, 1432
RsaNI	GTAC	8 cut(s)	102, 118, 211, 313, 899, 1075, 1294, 1431
Rsr2I	CGGWCCG	3 cut(s)	148, 1255, 1307
RsrII	CGGWCCG	3 cut(s)	148, 1255, 1307
SacI	GAGCTC	1 cut(s)	501
SaqAI	TTAA	3 cut(s)	378, 1164, 1248
SatI	GCNGC	2 cut(s)	128, 1350
Sau3AI	GATC	2 cut(s)	441, 573
Sau96I	GGNCC	8 cut(s)	136, 137, 148, 176, 399, 1255, 1299, 1307
SchI	GAGTC	3 cut(s)	1031, 1148, 1340
ScrFI	CCNGG	8 cut(s)	27, 111, 134, 155, 173, 632, 734, 1126
SduI	GDGCHC	4 cut(s)	20, 140, 221, 501
SfaNI	GCATC	1 cut(s)	39
Sfr274I	CTCGAG	1 cut(s)	1448
SgrAI	CRCCGGYG	1 cut(s)	386
SinI	GGWCC	6 cut(s)	148, 176, 399, 1255, 1299, 1307
SlaI	CTCGAG	1 cut(s)	1448
SmlI	CTYRAG	2 cut(s)	394, 1448
SmoI	CTYRAG	2 cut(s)	394, 1448
Sse9I	AATT	2 cut(s)	879, 1112
SsiI	CCGC	5 cut(s)	128, 151, 229, 254, 1310
SspMI	CTAG	1 cut(s)	776
SstI	GAGCTC	1 cut(s)	501
StyD4I	CCNGG	8 cut(s)	25, 109, 132, 153, 171, 630, 732, 1124
StyI	CCWWGG	1 cut(s)	456
TaaI	ACNGT	4 cut(s)	39, 45, 223, 709
TaiI	ACGT	4 cut(s)	119, 270, 282, 546
TaqI	TCGA	6 cut(s)	294, 321, 336, 1035, 1230, 1449
TasI	AATT	2 cut(s)	879, 1112
TatI	WGTACW	3 cut(s)	210, 898, 1293
TauI	GCSGC	1 cut(s)	130
TfiI	GAWTC	2 cut(s)	742, 1412
Tru1I	TTAA	3 cut(s)	378, 1164, 1248
Tru9I	TTAA	3 cut(s)	378, 1164, 1248
TscAI	CASTG	4 cut(s)	508, 787, 1349, 1378
TseFI	GTSAC	3 cut(s)	39, 45, 1363
TseI	GCWGC	1 cut(s)	1349
Tsp45I	GTSAC	3 cut(s)	39, 45, 1363
TspDTI	ATGAA	5 cut(s)	355, 580, 1248, 1252, 1280
TspGWI	ACGGA	3 cut(s)	89, 1057, 1320
TspRI	CASTG	4 cut(s)	508, 787, 1349, 1378
Van91I	CCANNNNNTGG	1 cut(s)	486
VpaK11BI	GGWCC	6 cut(s)	148, 176, 399, 1255, 1299, 1307
XagI	CCTNNNNNAGG	1 cut(s)	1451
XceI	RCATGY	1 cut(s)	319
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1384
XhoI	CTCGAG	1 cut(s)	1448
XmnI	GAANNNNTTC	2 cut(s)	1133, 1226
XspI	CTAG	1 cut(s)	776
ZraI	GACGTC	1 cut(s)	544
Zsp2I	ATGCAT	1 cut(s)	916

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.