Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2B

Orthogroup ID

OG0012169

Physical Location & Seq

Genomic Coordinates Forward (+)

75864792 .. 75867734

Gene Structure 2943 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2BG549900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGTCTGCTTTCATGATGAGCATCACTCCAGGTATACCCTCTTCTTCTTCTTCTTCTTCTTCTTCACCATCACACGTAGTGACTTCTTCACCACCGCCGACGTCACTTCCCGTCCGTACGATCCCGGGGGCGTACGGCCTTCCGTTGCTGGGCCCCATCTCCGACCGGCTGGACTACTTCTGGTTCCAGGGACCGGAGACGTTCTTCAAGAAAAGAACGGACAAGTACAAGAGTACGGTGTTCCGGACCAATGTCCCTCCGAGTTTCCCGCTGTTCTTGGGTGTTAACCCCAACGTTGTGGCCGTGCTGGACTGCAAGTCGTTCTCGTATCTCTTCGACATGGACGTCGTCGAGAAGAAGAACTGTCTGGTCGGCGACTTCATGCCCAGTGTTAAGTTCACCGGTGACCTCAGGACCTGTGCTTACCTTGATACTTCTGAACCTCAACACGCCCAGATCAAGAGCTTTGCCATGGACATCTTGAAACAAAGCTCCAAGATATGGGTAACTGAGCTCACTGCCAACCTCTCCACATTTTGGGACACGATAGATGCAGATGTTGCCAAGGATGGAAAGGCTAGCTATTTGATCCCACTCCAGAAGTCCTTCTTCAATTTCCTCACAAAATGCTTAGTTGGAGCTGACGCTTCGAACTCGCCCAAAATAGCCGAGTCTGGCTACGCTATGCTCGACCGCTGGCTCGCCCTCCAGCTCCTCCCCACTGTGAAGATCGGTGTGCTTCAGCCTCTCGAGGAGATTTTCCTCCATTCCTTTGCATACCCATCTTTTCTTGTGAGTGGAGACTACAAAAAGGTTTACCAGTTTATAGAAGAATATGGTAAAGAAGTAGTAAACAGAGGTCAAACCGAGTTCGGACTCAGTAAAGAAGAAGCTATTCACAACCTTCTGTTTGTGCTTGGCTTCAACGCATTTGGTGGGTTCTCAGTGTTTCTTCCGAGTTTAGTAAGCGCAATAGCCAGTGACACCACTGGCTTACAACAAAGACTAGTCAAAGAAGTCAGGGAAAATGCCGGTTCAACTCTGAGTTTTGAATCGGTGAAAGACTTGCAACTCGTTCAGTCTGTGGTGTATGAAACACTCCGGCTCAACCCCCCAGTTCCTCTCCAATTCGCCCGGGCCAGAAAGGACTTCCAACTCAGTTCGCATGACTCGGCCTTTGAGATCAAGAAAGGAGAGTTGCTTTGTGGGTTTCAAACACAGGTCATGAGGGACGGGAAGATCTTTGATGATCCGGAGACTTTTAAACCGGACCGGTTCCTGGAAAACAAAGAGTTACAGAATTATTTGTACTGGTCCAACGGACCGCAAACTGGTTCACCCAGCGCCTCAAACAAACAGTGCCCGGGCAAGGACACAGTGACCCTCACTGCTTGTTTGTTCGTTGCTTATACATTTCAAAGATATGACTCAATCACCGGGACTTCTTCCTCTATTACAGCCGTTGAGAAGGCCAAGCAGGAAGCAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006631 GO:0006950 GO:0008150 GO:0008152 GO:0008202 GO:0009507 GO:0009526 GO:0009536 GO:0009611 GO:0009941 GO:0009987 GO:0016125 GO:0016491 GO:0019752 GO:0031967 GO:0031975 GO:0032787 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0055114 GO:0071704 GO:1901360 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko00592 ko01110 map00592 map01110

Pfam Domains

Protein Families

p450

Protein Analysis

496

Amino Acids

55.01

Weight (kDa)

6.06

Isoelectric Point (pI)

44.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	332 - 434	4.6e-17	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012169)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G15440
fragaria_vesca	FvH4_6g42740
malus_domestica	MD09G1071400.v1.1 MD17G1100800.v1.1
prunus_persica	Prupe.3G213800_v2.0.a1
pyrus_communis	pycom09g03470 pycom17g09550
rosa_chinensis	RchiOBHm_Chr2g0158631
rosa_laevigata	RLG00000021101
rosa_multiflora	Rmu_co8067608.1_g000001 Rmu_sc0004188.1_g000015 Rmu_sc0034116.1_g000001
rosa_roxburghii	Rroxscaffold_2G00091160
rosa_rugosa	Rorug02G0471000
rosa_samantha	Rh2AG536700 Rh2BG549900 Rh2CG520400 Rh2DG559500
rosa_wichuraiana	Rw2G044430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	344
AatII	GACGTC	2 cut(s)	104, 348
AccB7I	CCANNNNNTGG	2 cut(s)	501, 537
AccI	GTMKAC	1 cut(s)	34
AccIII	TCCGGA	2 cut(s)	243, 1252
AciI	CCGC	4 cut(s)	95, 269, 694, 1325
AclI	AACGTT	1 cut(s)	294
AclWI	GGATC	3 cut(s)	115, 583, 1244
AcoI	YGGCCR	1 cut(s)	300
AcuI	CTGAAG	1 cut(s)	725
AcyI	GRCGYC	2 cut(s)	101, 345
AdeI	CACNNNGTG	1 cut(s)	79
AfaI	GTAC	5 cut(s)	118, 134, 227, 235, 1310
AfiI	CCNNNNNNNGG	7 cut(s)	149, 193, 501, 537, 1279, 1331, 1369
AflIII	ACRYGT	1 cut(s)	73
AgeI	ACCGGT	2 cut(s)	401, 1272
AgsI	TTSAA	8 cut(s)	208, 484, 613, 925, 1038, 1052, 1214, 1418
AhdI	GACNNNNNGTC	1 cut(s)	316
AhlI	ACTAGT	1 cut(s)	1006
AjnI	CCWGG	3 cut(s)	28, 186, 1278
AluBI	AGCT	7 cut(s)	465, 492, 514, 582, 641, 712, 893
AluI	AGCT	7 cut(s)	465, 492, 514, 582, 641, 712, 893
Alw21I	GWGCWC	1 cut(s)	516
Alw26I	GTCTC	3 cut(s)	191, 795, 1250
AlwI	GGATC	3 cut(s)	115, 583, 1244
AlwNI	CAGNNNCTG	1 cut(s)	417
Ama87I	CYCGRG	4 cut(s)	124, 749, 1134, 1363
Aor13HI	TCCGGA	2 cut(s)	243, 1252
AoxI	GGCC	6 cut(s)	136, 151, 300, 1137, 1173, 1470
ApaI	GGGCCC	1 cut(s)	155
ApeKI	GCWGC	1 cut(s)	1484
ArsI	GACNNNNNNTTYG	2 cut(s)	1160, 1192
AsiGI	ACCGGT	2 cut(s)	401, 1272
Asp700I	GAANNNNTTC	2 cut(s)	605, 894
AspLEI	GCGC	2 cut(s)	971, 1346
AspS9I	GGNCC	9 cut(s)	151, 152, 191, 246, 414, 1137, 1270, 1314, 1322
AsuC2I	CCSGG	7 cut(s)	125, 126, 1135, 1136, 1364, 1365, 1438
AsuHPI	GGTGA	7 cut(s)	57, 81, 391, 416, 1069, 1329, 1426
AsuII	TTCGAA	1 cut(s)	650
AsuNHI	GCTAGC	1 cut(s)	578
AvaI	CYCGRG	4 cut(s)	124, 749, 1134, 1363
AvaII	GGWCC	6 cut(s)	191, 246, 414, 1270, 1314, 1322
AxyI	CCTNAGG	1 cut(s)	410
BaeGI	GKGCMC	2 cut(s)	155, 1364
BanII	GRGCYC	2 cut(s)	155, 516
BarI	GAAGNNNNNNTAC	2 cut(s)	25, 57
Bbv12I	GWGCWC	1 cut(s)	516
BccI	CCATC	4 cut(s)	76, 164, 563, 790
BceAI	ACGGC	3 cut(s)	151, 287, 1445
BciT130I	CCWGG	3 cut(s)	30, 188, 1280
BcnI	CCSGG	7 cut(s)	125, 126, 1135, 1136, 1364, 1365, 1438
BcoDI	GTCTC	3 cut(s)	191, 795, 1250
BcuI	ACTAGT	1 cut(s)	1006
BfaI	CTAG	2 cut(s)	579, 1007
BfoI	RGCGCY	1 cut(s)	1347
BglII	AGATCT	1 cut(s)	1239
BisI	GCNGC	1 cut(s)	1485
BlsI	GCNGC	1 cut(s)	1486
Bme18I	GGWCC	6 cut(s)	191, 246, 414, 1270, 1314, 1322
BmeRI	GACNNNNNGTC	1 cut(s)	316
BmeT110I	CYCGRG	4 cut(s)	124, 749, 1134, 1363
BmgT120I	GGNCC	9 cut(s)	151, 152, 191, 246, 414, 1137, 1270, 1314, 1322
BmiI	GGNNCC	5 cut(s)	153, 154, 185, 192, 1277
BmrI	ACTGGG	2 cut(s)	381, 1109
BmsI	GCATC	2 cut(s)	30, 541
BmtI	GCTAGC	1 cut(s)	582
BmuI	ACTGGG	2 cut(s)	381, 1109
BoxI	GACNNNNGTC	1 cut(s)	251
BplI	GAGNNNNNCTC	2 cut(s)	10, 42
BpmI	CTGGAG	3 cut(s)	12, 581, 692
Bpu14I	TTCGAA	1 cut(s)	650
BpuMI	CCSGG	7 cut(s)	125, 126, 1135, 1136, 1364, 1365, 1438
BsaAI	YACGTR	1 cut(s)	76
BsaBI	GATNNNNATC	1 cut(s)	20
BsaHI	GRCGYC	2 cut(s)	101, 345
BsaJI	CCNNGG	7 cut(s)	124, 125, 187, 471, 564, 1134, 1363
BsaWI	WCCGGW	6 cut(s)	193, 243, 401, 1252, 1267, 1272
Bsc4I	CCNNNNNNNGG	7 cut(s)	149, 193, 501, 537, 1279, 1331, 1369
Bse118I	RCCGGY	4 cut(s)	165, 401, 1031, 1272
Bse1I	ACTGG	7 cut(s)	387, 820, 978, 994, 1115, 1316, 1336
Bse21I	CCTNAGG	1 cut(s)	410
Bse8I	GATNNNNATC	1 cut(s)	20
BseAI	TCCGGA	2 cut(s)	243, 1252
BseBI	CCWGG	3 cut(s)	30, 188, 1280
BseDI	CCNNGG	7 cut(s)	124, 125, 187, 471, 564, 1134, 1363
BseGI	GGATG	1 cut(s)	574
BseJI	GATNNNNATC	1 cut(s)	20
BseLI	CCNNNNNNNGG	7 cut(s)	149, 193, 501, 537, 1279, 1331, 1369
BseMII	CTCAG	6 cut(s)	424, 501, 892, 957, 1034, 1171
BseNI	ACTGG	7 cut(s)	387, 820, 978, 994, 1115, 1316, 1336
BseRI	GAGGAG	2 cut(s)	704, 767
BseSI	GKGCMC	2 cut(s)	155, 1364
BseYI	CCCAGC	2 cut(s)	148, 1340
Bsh1285I	CGRYCG	2 cut(s)	166, 694
BshFI	GGCC	6 cut(s)	138, 153, 302, 1139, 1175, 1472
BshTI	ACCGGT	2 cut(s)	401, 1272
BsiEI	CGRYCG	2 cut(s)	166, 694
BsiHKAI	GWGCWC	1 cut(s)	516
BsiHKCI	CYCGRG	4 cut(s)	124, 749, 1134, 1363
BsiWI	CGTACG	2 cut(s)	116, 132
BslFI	GGGAC	5 cut(s)	204, 239, 554, 1244, 1453
BslI	CCNNNNNNNGG	7 cut(s)	149, 193, 501, 537, 1279, 1331, 1369
BsmAI	GTCTC	3 cut(s)	191, 795, 1250
BsmBI	CGTCTC	1 cut(s)	191
BsmFI	GGGAC	5 cut(s)	204, 239, 554, 1244, 1453
BsnI	GGCC	6 cut(s)	138, 153, 302, 1139, 1175, 1472
BsoBI	CYCGRG	4 cut(s)	124, 749, 1134, 1363
Bsp119I	TTCGAA	1 cut(s)	650
Bsp120I	GGGCCC	1 cut(s)	151
Bsp1286I	GDGCHC	3 cut(s)	155, 516, 1364
Bsp13I	TCCGGA	2 cut(s)	243, 1252
Bsp143I	GATC	7 cut(s)	120, 456, 588, 729, 1182, 1239, 1249
Bsp19I	CCATGG	1 cut(s)	471
BspACI	CCGC	4 cut(s)	95, 269, 694, 1325
BspANI	GGCC	6 cut(s)	138, 153, 302, 1139, 1175, 1472
BspCNI	CTCAG	6 cut(s)	423, 502, 891, 956, 1035, 1170
BspEI	TCCGGA	2 cut(s)	243, 1252
BspHI	TCATGA	2 cut(s)	12, 1224
BspLI	GGNNCC	5 cut(s)	153, 154, 185, 192, 1277
BspOI	GCTAGC	1 cut(s)	582
BspPI	GGATC	3 cut(s)	115, 583, 1244
BspT104I	TTCGAA	1 cut(s)	650
BsrFI	RCCGGY	4 cut(s)	165, 401, 1031, 1272
BsrI	ACTGG	7 cut(s)	387, 820, 978, 994, 1115, 1316, 1336
BssAI	RCCGGY	4 cut(s)	165, 401, 1031, 1272
BssECI	CCNNGG	7 cut(s)	124, 125, 187, 471, 564, 1134, 1363
BssMI	GATC	7 cut(s)	120, 456, 588, 729, 1182, 1239, 1249
BssNAI	GTATAC	1 cut(s)	35
BssNI	GRCGYC	2 cut(s)	101, 345
BssT1I	CCWWGG	2 cut(s)	471, 564
Bst1107I	GTATAC	1 cut(s)	35
Bst2UI	CCWGG	3 cut(s)	30, 188, 1280
Bst4CI	ACNGT	5 cut(s)	238, 365, 724, 1359, 1378
Bst6I	CTCTTC	2 cut(s)	46, 338
BstACI	GRCGYC	2 cut(s)	101, 345
BstAPI	GCANNNNNTGC	1 cut(s)	560
BstBAI	YACGTR	1 cut(s)	76
BstBI	TTCGAA	1 cut(s)	650
BstC8I	GCNNGC	3 cut(s)	580, 698, 702
BstDEI	CTNAG	7 cut(s)	410, 510, 631, 878, 943, 1043, 1157
BstDSI	CCRYGG	1 cut(s)	471
BstEII	GGTNACC	1 cut(s)	404
BstF5I	GGATG	1 cut(s)	574
BstH2I	RGCGCY	1 cut(s)	1347
BstHHI	GCGC	2 cut(s)	971, 1346
BstKTI	GATC	7 cut(s)	123, 459, 591, 732, 1185, 1242, 1252
BstMAI	GTCTC	3 cut(s)	191, 795, 1250
BstMBI	GATC	7 cut(s)	120, 456, 588, 729, 1182, 1239, 1249
BstMCI	CGRYCG	2 cut(s)	166, 694
BstMWI	GCNNNNNNNGC	1 cut(s)	560
BstNI	CCWGG	3 cut(s)	30, 188, 1280
BstPAI	GACNNNNGTC	1 cut(s)	251
BstPI	GGTNACC	1 cut(s)	404
BstSLI	GKGCMC	2 cut(s)	155, 1364
BstX2I	RGATCY	1 cut(s)	1239
BstXI	CCANNNNNNTGG	1 cut(s)	298
BstYI	RGATCY	1 cut(s)	1239
BstZ17I	GTATAC	1 cut(s)	35
Bsu36I	CCTNAGG	1 cut(s)	410
BsuRI	GGCC	6 cut(s)	138, 153, 302, 1139, 1175, 1472
BtgI	CCRYGG	1 cut(s)	471
BtsCI	GGATG	1 cut(s)	574
BtsI	GCAGTG	2 cut(s)	516, 1386
BtsIMutI	CAGTG	9 cut(s)	394, 516, 720, 951, 985, 987, 1364, 1383, 1386
Cac8I	GCNNGC	3 cut(s)	580, 698, 702
CaiI	CAGNNNCTG	1 cut(s)	417
CciI	TCATGA	2 cut(s)	12, 1224
CfoI	GCGC	2 cut(s)	971, 1346
Cfr10I	RCCGGY	4 cut(s)	165, 401, 1031, 1272
Cfr13I	GGNCC	9 cut(s)	151, 152, 191, 246, 414, 1137, 1270, 1314, 1322
Cfr9I	CCCGGG	3 cut(s)	124, 1134, 1363
CpoI	CGGWCCG	2 cut(s)	1270, 1322
CseI	GACGC	1 cut(s)	653
Csp6I	GTAC	5 cut(s)	117, 133, 226, 234, 1309
CspAI	ACCGGT	2 cut(s)	401, 1272
CspI	CGGWCCG	2 cut(s)	1270, 1322
CviAII	CATG	7 cut(s)	13, 340, 382, 472, 1166, 1225, 1488
CviQI	GTAC	5 cut(s)	117, 133, 226, 234, 1309
DdeI	CTNAG	7 cut(s)	410, 510, 631, 878, 943, 1043, 1157
DpnI	GATC	7 cut(s)	122, 458, 590, 731, 1184, 1241, 1251
DpnII	GATC	7 cut(s)	120, 456, 588, 729, 1182, 1239, 1249
DraI	TTTAAA	1 cut(s)	1264
DraIII	CACNNNGTG	1 cut(s)	79
DrdI	GACNNNNNNGTC	1 cut(s)	344
DriI	GACNNNNNGTC	1 cut(s)	316
DseDI	GACNNNNNNGTC	1 cut(s)	344
EaeI	YGGCCR	1 cut(s)	300
Eam1104I	CTCTTC	2 cut(s)	46, 338
Eam1105I	GACNNNNNGTC	1 cut(s)	316
EarI	CTCTTC	2 cut(s)	46, 338
Ecl136II	GAGCTC	1 cut(s)	514
Eco130I	CCWWGG	2 cut(s)	471, 564
Eco24I	GRGCYC	2 cut(s)	155, 516
Eco47I	GGWCC	6 cut(s)	191, 246, 414, 1270, 1314, 1322
Eco53kI	GAGCTC	1 cut(s)	514
Eco57I	CTGAAG	1 cut(s)	725
Eco81I	CCTNAGG	1 cut(s)	410
Eco88I	CYCGRG	4 cut(s)	124, 749, 1134, 1363
Eco91I	GGTNACC	1 cut(s)	404
EcoICRI	GAGCTC	1 cut(s)	514
EcoO109I	RGGNCCY	2 cut(s)	152, 414
EcoO65I	GGTNACC	1 cut(s)	404
EcoRII	CCWGG	3 cut(s)	28, 186, 1278
EcoT14I	CCWWGG	2 cut(s)	471, 564
EcoT38I	GRGCYC	2 cut(s)	155, 516
ErhI	CCWWGG	2 cut(s)	471, 564
Esp3I	CGTCTC	1 cut(s)	191
FaeI	CATG	7 cut(s)	16, 343, 385, 475, 1169, 1228, 1491
FalI	AAGNNNNNCTT	2 cut(s)	593, 625
FaqI	GGGAC	5 cut(s)	204, 239, 554, 1244, 1453
FatI	CATG	7 cut(s)	12, 339, 381, 471, 1165, 1224, 1487
FauI	CCCGC	1 cut(s)	276
FblI	GTMKAC	1 cut(s)	34
Fnu4HI	GCNGC	1 cut(s)	1485
FokI	GGATG	1 cut(s)	581
FriOI	GRGCYC	2 cut(s)	155, 516
Fsp4HI	GCNGC	1 cut(s)	1485
FspBI	CTAG	2 cut(s)	579, 1007
GlaI	GCGC	2 cut(s)	970, 1345
GluI	GCNGC	1 cut(s)	1485
GsaI	CCCAGC	2 cut(s)	152, 1344
GsuI	CTGGAG	3 cut(s)	12, 581, 692
HaeII	RGCGCY	1 cut(s)	1347
HaeIII	GGCC	6 cut(s)	138, 153, 302, 1139, 1175, 1472
HgaI	GACGC	1 cut(s)	653
HhaI	GCGC	2 cut(s)	971, 1346
Hin1I	GRCGYC	2 cut(s)	101, 345
Hin1II	CATG	7 cut(s)	16, 343, 385, 475, 1169, 1228, 1491
Hin6I	GCGC	2 cut(s)	969, 1344
HinP1I	GCGC	2 cut(s)	969, 1344
HincII	GTYRAC	1 cut(s)	286
HindII	GTYRAC	1 cut(s)	286
HinfI	GANTC	5 cut(s)	671, 876, 1052, 1169, 1427
HpaI	GTTAAC	1 cut(s)	286
HphI	GGTGA	7 cut(s)	57, 81, 391, 416, 1069, 1329, 1426
Hpy166II	GTNNAC	6 cut(s)	35, 286, 399, 817, 853, 1337
Hpy188I	TCNGA	6 cut(s)	163, 261, 439, 875, 957, 1044
Hpy8I	GTNNAC	6 cut(s)	35, 286, 399, 817, 853, 1337
Hpy99I	CGWCG	3 cut(s)	103, 350, 353
HpyAV	CCTTC	4 cut(s)	149, 616, 914, 1462
HpyCH4III	ACNGT	5 cut(s)	238, 365, 724, 1359, 1378
HpyCH4IV	ACGT	5 cut(s)	75, 101, 200, 294, 345
HpyCH4V	TGCA	4 cut(s)	315, 554, 776, 1069
HpyF10VI	GCNNNNNNNGC	1 cut(s)	560
HpyF3I	CTNAG	7 cut(s)	410, 510, 631, 878, 943, 1043, 1157
HpySE526I	ACGT	5 cut(s)	75, 101, 200, 294, 345
Hsp92I	GRCGYC	2 cut(s)	101, 345
Hsp92II	CATG	7 cut(s)	16, 343, 385, 475, 1169, 1228, 1491
HspAI	GCGC	2 cut(s)	969, 1344
Kpn2I	TCCGGA	2 cut(s)	243, 1252
KspAI	GTTAAC	1 cut(s)	286
Kzo9I	GATC	7 cut(s)	120, 456, 588, 729, 1182, 1239, 1249
LmnI	GCTCC	3 cut(s)	497, 638, 717
LweI	GCATC	2 cut(s)	30, 541
MaeI	CTAG	2 cut(s)	579, 1007
MaeII	ACGT	5 cut(s)	75, 101, 200, 294, 345
MaeIII	GTNAC	7 cut(s)	79, 102, 404, 505, 980, 1293, 1378
MalI	GATC	7 cut(s)	122, 458, 590, 731, 1184, 1241, 1251
MboI	GATC	7 cut(s)	120, 456, 588, 729, 1182, 1239, 1249
MflI	RGATCY	1 cut(s)	1239
MhlI	GDGCHC	3 cut(s)	155, 516, 1364
MluCI	AATT	3 cut(s)	613, 1127, 1300
MlyI	GAGTC	4 cut(s)	680, 870, 1163, 1421
MmeI	TCCRAC	4 cut(s)	186, 616, 1177, 1341
MroI	TCCGGA	2 cut(s)	243, 1252
MroXI	GAANNNNTTC	2 cut(s)	605, 894
MseI	TTAA	3 cut(s)	285, 393, 1263
MspA1I	CMGCKG	2 cut(s)	271, 696
MvaI	CCWGG	3 cut(s)	30, 188, 1280
MwoI	GCNNNNNNNGC	1 cut(s)	560
NciI	CCSGG	7 cut(s)	125, 126, 1135, 1136, 1364, 1365, 1438
NcoI	CCATGG	1 cut(s)	471
NdeII	GATC	7 cut(s)	120, 456, 588, 729, 1182, 1239, 1249
NheI	GCTAGC	1 cut(s)	578
NlaIII	CATG	7 cut(s)	16, 343, 385, 475, 1169, 1228, 1491
NlaIV	GGNNCC	5 cut(s)	153, 154, 185, 192, 1277
NmeAIII	GCCGAG	2 cut(s)	694, 1151
NmuCI	GTSAC	5 cut(s)	79, 102, 404, 980, 1378
NspV	TTCGAA	1 cut(s)	650
PaeR7I	CTCGAG	1 cut(s)	749
PagI	TCATGA	2 cut(s)	12, 1224
PcsI	WCGNNNNNNNCGW	3 cut(s)	300, 342, 687
PdmI	GAANNNNTTC	2 cut(s)	605, 894
PfeI	GAWTC	1 cut(s)	1052
Pfl23II	CGTACG	2 cut(s)	116, 132
PflFI	GACNNNGTC	1 cut(s)	347
PflMI	CCANNNNNTGG	2 cut(s)	501, 537
PfoI	TCCNGGA	1 cut(s)	1278
PinAI	ACCGGT	2 cut(s)	401, 1272
PkrI	GCNGC	1 cut(s)	1486
PleI	GAGTC	4 cut(s)	679, 870, 1163, 1421
PpsI	GAGTC	4 cut(s)	679, 870, 1163, 1421
Ppu21I	YACGTR	1 cut(s)	76
PpuMI	RGGWCCY	1 cut(s)	414
PshAI	GACNNNNGTC	1 cut(s)	251
Psp124BI	GAGCTC	1 cut(s)	516
Psp1406I	AACGTT	1 cut(s)	294
Psp5II	RGGWCCY	1 cut(s)	414
Psp6I	CCWGG	3 cut(s)	28, 186, 1278
PspEI	GGTNACC	1 cut(s)	404
PspFI	CCCAGC	2 cut(s)	148, 1340
PspGI	CCWGG	3 cut(s)	28, 186, 1278
PspLI	CGTACG	2 cut(s)	116, 132
PspN4I	GGNNCC	5 cut(s)	153, 154, 185, 192, 1277
PspOMI	GGGCCC	1 cut(s)	151
PspPI	GGNCC	9 cut(s)	151, 152, 191, 246, 414, 1137, 1270, 1314, 1322
PspPPI	RGGWCCY	1 cut(s)	414
PstNI	CAGNNNCTG	1 cut(s)	417
PsuI	RGATCY	1 cut(s)	1239
PsyI	GACNNNGTC	1 cut(s)	347
RsaI	GTAC	5 cut(s)	118, 134, 227, 235, 1310
RsaNI	GTAC	5 cut(s)	117, 133, 226, 234, 1309
Rsr2I	CGGWCCG	2 cut(s)	1270, 1322
RsrII	CGGWCCG	2 cut(s)	1270, 1322
SacI	GAGCTC	1 cut(s)	516
SaqAI	TTAA	3 cut(s)	285, 393, 1263
SatI	GCNGC	1 cut(s)	1485
Sau3AI	GATC	7 cut(s)	120, 456, 588, 729, 1182, 1239, 1249
Sau96I	GGNCC	9 cut(s)	151, 152, 191, 246, 414, 1137, 1270, 1314, 1322
SchI	GAGTC	4 cut(s)	680, 870, 1163, 1421
SduI	GDGCHC	3 cut(s)	155, 516, 1364
SfaNI	GCATC	2 cut(s)	30, 541
Sfr274I	CTCGAG	1 cut(s)	749
SfuI	TTCGAA	1 cut(s)	650
SgrAI	CRCCGGYG	1 cut(s)	401
SinI	GGWCC	6 cut(s)	191, 246, 414, 1270, 1314, 1322
SlaI	CTCGAG	1 cut(s)	749
SmaI	CCCGGG	3 cut(s)	126, 1136, 1365
SmlI	CTYRAG	1 cut(s)	749
SmoI	CTYRAG	1 cut(s)	749
SpeI	ACTAGT	1 cut(s)	1006
SrfI	GCCCGGGC	2 cut(s)	1136, 1365
Sse9I	AATT	3 cut(s)	613, 1127, 1300
SsiI	CCGC	4 cut(s)	95, 269, 694, 1325
SspMI	CTAG	2 cut(s)	579, 1007
SstI	GAGCTC	1 cut(s)	516
StyI	CCWWGG	2 cut(s)	471, 564
TaaI	ACNGT	5 cut(s)	238, 365, 724, 1359, 1378
TaiI	ACGT	5 cut(s)	78, 104, 203, 297, 348
TaqI	TCGA	5 cut(s)	336, 351, 650, 690, 750
TasI	AATT	3 cut(s)	613, 1127, 1300
TatI	WGTACW	2 cut(s)	225, 1308
TfiI	GAWTC	1 cut(s)	1052
Tru1I	TTAA	3 cut(s)	285, 393, 1263
Tru9I	TTAA	3 cut(s)	285, 393, 1263
TscAI	CASTG	9 cut(s)	394, 523, 727, 951, 985, 994, 1364, 1383, 1393
TseFI	GTSAC	5 cut(s)	79, 102, 404, 980, 1378
TseI	GCWGC	1 cut(s)	1484
Tsp45I	GTSAC	5 cut(s)	79, 102, 404, 980, 1378
TspDTI	ATGAA	2 cut(s)	370, 1107
TspGWI	ACGGA	4 cut(s)	104, 132, 233, 1335
TspMI	CCCGGG	3 cut(s)	124, 1134, 1363
TspRI	CASTG	9 cut(s)	394, 523, 727, 951, 985, 994, 1364, 1383, 1393
Tth111I	GACNNNGTC	1 cut(s)	347
Van91I	CCANNNNNTGG	2 cut(s)	501, 537
VpaK11BI	GGWCC	6 cut(s)	191, 246, 414, 1270, 1314, 1322
XhoI	CTCGAG	1 cut(s)	749
XmaI	CCCGGG	3 cut(s)	124, 1134, 1363
XmiI	GTMKAC	1 cut(s)	34
XmnI	GAANNNNTTC	2 cut(s)	605, 894
XspI	CTAG	2 cut(s)	579, 1007
ZraI	GACGTC	2 cut(s)	102, 346

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.