pycom09g05620

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012642
Physical Location & Seq
Forward (+)
4174604 .. 4179087
4484 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1668 bp
ATGGCGGACAATTCAGTATCTGAGCTTCAGCTTGGAGAGGAATCAACTACGGAGAGCTCATCTGATCAGCACCAGATTCATGAACAAAAGGAAAAGAATGAAGAAACCAGTTCGTCTAGACGGCCAAACCTCTCCTCGTTGCAAATACCGGCAAGGACATTGGAAACCAGTTCGTCTGCTTGTACGAGAATTGATATTCCCTCCACACCAAGTCCAAGTTCTAGTAAAGTTGGACTACCGCCTAGGCCACATTCAGCTAAGATCAAATCAACCGTGAAAAATCTGTTTCCTCAAAAAAGCTTCCGGGTGAAAAATTTGCCTCTGGATGGCGAGAAGACGATTCTCATTATGCCGGATACATCACCGTCAGATAGTCCGTTGGAAAAGCCTTCTACTTCACGGTCTTTCTCGCTCAATAAGCTTTTCTTCTCTACATCAACAAAAGGGACTCATTCTTTACCAGTTACACCAAGTGCAAATCTGGGTTCTGAGATTGGGCAGGGAAGACAAGTGGAGAGTCATTCTGATTCTAGAATTGAAGTTAAGCATCATATGTCCCGCTCTTTCTCAGTTCCAGTAAATATTGCGACCAGAAGTTTAAGGCGGATGGATTCTGGATGCATGATCCGTGTAATTTCAGCAACTCCACGTCCTTCAGCAGTTGAGCGTGCCTCACCAAATGCTGACCCAGCACTGGAAACGGCCACAGCCACTGAAGATTCGGGTGAAGATATTCCTGAAGAAGAAGCAGTTTGCAGGATTTGTTTGGTTGAGCTCGCAGAAGGTGGTGATTCTCTTAAAATGGAGTGCAGCTGCAAGGGAGAGCTAGCACTTGCTCACAAAGATTGTGCAGTAAAGTGGTTTAGCATTAAAGGGAACAAAACCTGTGACGTCTGCAAGCAGGATGTCCGAAACTTACCTGTAACACTACTCAAAATACACAATCCTCAAACTGTTATTAGACGACCACCAACAATAATGGAGCAGAGGGAAGTCCATCATTACAGGGTCTGGCAGGACATACCGGTTCTTGTCCTGGTCAGCATGCTTGCGTATTTCTGCTTTCTGGAGCAACTTCTGGTATCTGACTTGGGCCCTCGTGCTCTTGCCGTTTCCTTACCCTTCTCTTGTGTTTTAGGTATCCTTTCATCTATGATTGCTTCCACAATGGTGAGCAGGAGCTACATATGGGCTTATGCCTCCTTCCAGTTTGCCATCGTGATCCTGTTTGCCCACATATTTTATACTGTACTTAATGTAAATCCAATTCTCTCAGTCCTCCTTTCCTCATTCACTGGCTTCGGGATTGCAATCAGCACGAATTCCCTTCTGGTTGAGTATCGTAGATGGAAAATCAGCAGACAACTGCAATCATCCTATCAACATGCTAGCAGTGCACTGCATCTGCATGAACTACAACTGCAGCAGCACCGGCAACAGCACCAGCAGTGGCTACAGCAAGAAGAGCAATATCAACGCCAATCCCAGCAACAACGACAGCAACAGTTGATGGAAGATTCACATGATCAGTACCAGCAAGTACAGCGACAGCTTCAGCAGCAAGAAGAGGAGCATCATCGACACCAACAGGAGCACCAGCACCAACGGTTGGATGGAGATCCAAATGCTGATGGTTCTAGGCCGCTAGAAGTTAGACAGGGCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

556

Amino Acids

62.44

Weight (kDa)

6.77

Isoelectric Point (pI)

72.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 252 - 299 7.2e-13 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 894
AccB7I CCANNNNNTGG 1 cut(s) 1609
AccBSI CCGCTC 1 cut(s) 561
AciI CCGC 5 cut(s) 5, 239, 559, 604, 1643
AclWI GGATC 3 cut(s) 619, 1216, 1613
AcoI YGGCCR 2 cut(s) 122, 702
AcsI RAATTY 2 cut(s) 313, 1321
AcuI CTGAAG 5 cut(s) 11, 639, 735, 759, 1538
AcyI GRCGYC 1 cut(s) 891
AdeI CACNNNGTG 1 cut(s) 473
AfaI GTAC 4 cut(s) 184, 1251, 1532, 1542
AfiI CCNNNNNNNGG 3 cut(s) 326, 694, 1609
AgeI ACCGGT 1 cut(s) 1024
AgsI TTSAA 1 cut(s) 539
AjiI CACGTC 1 cut(s) 650
AjnI CCWGG 1 cut(s) 1035
AjuI GAANNNNNNNTTGG 2 cut(s) 202, 234
AleI CACNNNNGTG 1 cut(s) 1169
Alw21I GWGCWC 5 cut(s) 59, 777, 1105, 1399, 1596
Alw44I GTGCAC 1 cut(s) 1395
AlwI GGATC 3 cut(s) 619, 1216, 1613
AlwNI CAGNNNCTG 3 cut(s) 20, 713, 1011
AoxI GGCC 5 cut(s) 122, 245, 702, 1093, 1640
ApaI GGGCCC 1 cut(s) 1097
ApaLI GTGCAC 1 cut(s) 1395
ApeKI GCWGC 5 cut(s) 810, 813, 1423, 1426, 1558
ApoI RAATTY 2 cut(s) 313, 1321
AsiGI ACCGGT 1 cut(s) 1024
Asp700I GAANNNNTTC 1 cut(s) 732
AspA2I CCTAGG 1 cut(s) 242
AspS9I GGNCC 2 cut(s) 1093, 1094
AsuC2I CCSGG 1 cut(s) 305
AsuHPI GGTGA 6 cut(s) 319, 354, 666, 737, 800, 1183
AsuNHI GCTAGC 2 cut(s) 826, 1388
AvrII CCTAGG 1 cut(s) 242
BaeGI GKGCMC 3 cut(s) 1097, 1399, 1664
BanII GRGCYC 3 cut(s) 59, 777, 1097
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BauI CACGAG 1 cut(s) 1098
BbsI GAAGAC 2 cut(s) 341, 511
Bbv12I GWGCWC 5 cut(s) 59, 777, 1105, 1399, 1596
BbvI GCAGC 5 cut(s) 800, 822, 1435, 1438, 1570
BccI CCATC 8 cut(s) 320, 601, 1005, 1223, 1341, 1504, 1607, 1625
BceAI ACGGC 3 cut(s) 137, 717, 1094
BciT130I CCWGG 1 cut(s) 1037
BciVI GTATCC 2 cut(s) 349, 1151
BclI TGATCA 2 cut(s) 64, 1525
BcnI CCSGG 1 cut(s) 305
BfaI CTAG 8 cut(s) 117, 222, 243, 531, 827, 1389, 1638, 1646
BfmI CTRYAG 2 cut(s) 1421, 1454
BfuI GTATCC 2 cut(s) 349, 1151
BisI GCNGC 6 cut(s) 811, 814, 1424, 1427, 1559, 1643
BlnI CCTAGG 1 cut(s) 242
BlsI GCNGC 6 cut(s) 812, 815, 1425, 1428, 1560, 1644
Bme1390I CCNGG 2 cut(s) 305, 1037
BmgBI CACGTC 1 cut(s) 650
BmgT120I GGNCC 2 cut(s) 1093, 1094
BmiI GGNNCC 1 cut(s) 1095
BmrFI CCNGG 2 cut(s) 305, 1037
BmsI GCATC 4 cut(s) 556, 608, 1411, 1582
BmtI GCTAGC 2 cut(s) 830, 1392
BpiI GAAGAC 2 cut(s) 341, 511
BplI GAGNNNNNCTC 2 cut(s) 656, 688
BpmI CTGGAG 1 cut(s) 1088
BpuMI CCSGG 1 cut(s) 305
BsaBI GATNNNNATC 2 cut(s) 1310, 1617
BsaHI GRCGYC 1 cut(s) 891
BsaJI CCNNGG 1 cut(s) 242
BsaWI WCCGGW 1 cut(s) 1024
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 694, 1609
Bse118I RCCGGY 3 cut(s) 148, 1024, 1431
Bse1I ACTGG 7 cut(s) 108, 168, 461, 575, 699, 1207, 1300
Bse8I GATNNNNATC 2 cut(s) 1310, 1617
BseBI CCWGG 1 cut(s) 1037
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 6 cut(s) 331, 612, 623, 910, 1373, 1618
BseJI GATNNNNATC 2 cut(s) 1310, 1617
BseLI CCNNNNNNNGG 3 cut(s) 326, 694, 1609
BseMII CTCAG 4 cut(s) 12, 480, 582, 1287
BseNI ACTGG 7 cut(s) 108, 168, 461, 575, 699, 1207, 1300
BseRI GAGGAG 2 cut(s) 124, 1583
BseSI GKGCMC 3 cut(s) 1097, 1399, 1664
BseXI GCAGC 5 cut(s) 800, 822, 1435, 1438, 1570
BseYI CCCAGC 2 cut(s) 688, 1485
BsgI GTGCAG 2 cut(s) 829, 870
BshFI GGCC 5 cut(s) 124, 247, 704, 1095, 1642
BshTI ACCGGT 1 cut(s) 1024
BsiHKAI GWGCWC 5 cut(s) 59, 777, 1105, 1399, 1596
BsiSI CCGG 5 cut(s) 149, 304, 353, 1025, 1432
BslFI GGGAC 2 cut(s) 460, 541
BslI CCNNNNNNNGG 3 cut(s) 326, 694, 1609
BsmFI GGGAC 2 cut(s) 460, 541
BsnI GGCC 5 cut(s) 124, 247, 704, 1095, 1642
Bsp120I GGGCCC 1 cut(s) 1093
Bsp1286I GDGCHC 7 cut(s) 59, 777, 1097, 1105, 1399, 1596, 1664
Bsp143I GATC 6 cut(s) 64, 261, 624, 1221, 1525, 1618
BspACI CCGC 5 cut(s) 5, 239, 559, 604, 1643
BspANI GGCC 5 cut(s) 124, 247, 704, 1095, 1642
BspCNI CTCAG 4 cut(s) 13, 481, 581, 1286
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 1 cut(s) 1095
BspMAI CTGCAG 1 cut(s) 1425
BspOI GCTAGC 2 cut(s) 830, 1392
BspPI GGATC 3 cut(s) 619, 1216, 1613
BspQI GCTCTTC 1 cut(s) 1458
BsrBI CCGCTC 1 cut(s) 561
BsrFI RCCGGY 3 cut(s) 148, 1024, 1431
BsrI ACTGG 7 cut(s) 108, 168, 461, 575, 699, 1207, 1300
BssAI RCCGGY 3 cut(s) 148, 1024, 1431
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 6 cut(s) 64, 261, 624, 1221, 1525, 1618
BssNI GRCGYC 1 cut(s) 891
BssSI CACGAG 1 cut(s) 1098
BssT1I CCWWGG 1 cut(s) 242
Bst2BI CACGAG 1 cut(s) 1098
Bst2UI CCWGG 1 cut(s) 1037
Bst4CI ACNGT 7 cut(s) 274, 366, 402, 955, 1249, 1506, 1608
Bst6I CTCTTC 2 cut(s) 1458, 1560
BstACI GRCGYC 1 cut(s) 891
BstC8I GCNNGC 7 cut(s) 669, 777, 828, 899, 1046, 1050, 1390
BstDEI CTNAG 5 cut(s) 21, 258, 489, 568, 1273
BstF5I GGATG 6 cut(s) 331, 612, 623, 910, 1373, 1618
BstKTI GATC 6 cut(s) 67, 264, 627, 1224, 1528, 1621
BstMBI GATC 6 cut(s) 64, 261, 624, 1221, 1525, 1618
BstMWI GCNNNNNNNGC 7 cut(s) 418, 689, 1394, 1432, 1465, 1543, 1558
BstNI CCWGG 1 cut(s) 1037
BstNSI RCATGY 2 cut(s) 1048, 1388
BstSCI CCNGG 2 cut(s) 303, 1035
BstSFI CTRYAG 2 cut(s) 1421, 1454
BstSLI GKGCMC 3 cut(s) 1097, 1399, 1664
BstV1I GCAGC 5 cut(s) 800, 822, 1435, 1438, 1570
BstV2I GAAGAC 2 cut(s) 341, 511
BstX2I RGATCY 1 cut(s) 1618
BstYI RGATCY 1 cut(s) 1618
BsuI GTATCC 2 cut(s) 349, 1151
BsuRI GGCC 5 cut(s) 124, 247, 704, 1095, 1642
BtrI CACGTC 1 cut(s) 650
BtsCI GGATG 6 cut(s) 331, 612, 623, 910, 1373, 1618
BtsI GCAGTG 3 cut(s) 1397, 1399, 1454
BtsIMutI CAGTG 6 cut(s) 692, 711, 1293, 1397, 1399, 1454
Cac8I GCNNGC 7 cut(s) 669, 777, 828, 899, 1046, 1050, 1390
CaiI CAGNNNCTG 3 cut(s) 20, 713, 1011
CciI TCATGA 1 cut(s) 79
Cfr10I RCCGGY 3 cut(s) 148, 1024, 1431
Cfr13I GGNCC 2 cut(s) 1093, 1094
Csp6I GTAC 4 cut(s) 183, 1250, 1531, 1541
CspAI ACCGGT 1 cut(s) 1024
CviAII CATG 6 cut(s) 80, 622, 1045, 1385, 1409, 1523
CviQI GTAC 4 cut(s) 183, 1250, 1531, 1541
DdeI CTNAG 5 cut(s) 21, 258, 489, 568, 1273
DpnI GATC 6 cut(s) 66, 263, 626, 1223, 1527, 1620
DpnII GATC 6 cut(s) 64, 261, 624, 1221, 1525, 1618
DraIII CACNNNGTG 1 cut(s) 473
EaeI YGGCCR 2 cut(s) 122, 702
Eam1104I CTCTTC 2 cut(s) 1458, 1560
EarI CTCTTC 2 cut(s) 1458, 1560
EciI GGCGGA 2 cut(s) 20, 619
Ecl136II GAGCTC 2 cut(s) 57, 775
Eco130I CCWWGG 1 cut(s) 242
Eco24I GRGCYC 3 cut(s) 59, 777, 1097
Eco53kI GAGCTC 2 cut(s) 57, 775
Eco57I CTGAAG 5 cut(s) 11, 639, 735, 759, 1538
EcoICRI GAGCTC 2 cut(s) 57, 775
EcoO109I RGGNCCY 1 cut(s) 1094
EcoRI GAATTC 1 cut(s) 1321
EcoRII CCWGG 1 cut(s) 1035
EcoT14I CCWWGG 1 cut(s) 242
EcoT22I ATGCAT 1 cut(s) 623
EcoT38I GRGCYC 3 cut(s) 59, 777, 1097
ErhI CCWWGG 1 cut(s) 242
FaeI CATG 6 cut(s) 83, 625, 1048, 1388, 1412, 1526
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 2 cut(s) 460, 541
FatI CATG 6 cut(s) 79, 621, 1044, 1384, 1408, 1522
FauI CCCGC 1 cut(s) 566
FauNDI CATATG 2 cut(s) 552, 1187
FbaI TGATCA 2 cut(s) 64, 1525
Fnu4HI GCNGC 6 cut(s) 811, 814, 1424, 1427, 1559, 1643
FokI GGATG 6 cut(s) 338, 619, 630, 917, 1360, 1625
FriOI GRGCYC 3 cut(s) 59, 777, 1097
Fsp4HI GCNGC 6 cut(s) 811, 814, 1424, 1427, 1559, 1643
FspBI CTAG 8 cut(s) 117, 222, 243, 531, 827, 1389, 1638, 1646
GluI GCNGC 6 cut(s) 811, 814, 1424, 1427, 1559, 1643
GsaI CCCAGC 2 cut(s) 692, 1489
GsuI CTGGAG 1 cut(s) 1088
HaeIII GGCC 5 cut(s) 124, 247, 704, 1095, 1642
HapII CCGG 5 cut(s) 149, 304, 353, 1025, 1432
Hin1I GRCGYC 1 cut(s) 891
Hin1II CATG 6 cut(s) 83, 625, 1048, 1388, 1412, 1526
HindIII AAGCTT 2 cut(s) 298, 419
HpaII CCGG 5 cut(s) 149, 304, 353, 1025, 1432
HphI GGTGA 6 cut(s) 319, 354, 666, 737, 800, 1183
Hpy166II GTNNAC 1 cut(s) 1397
Hpy188I TCNGA 7 cut(s) 22, 64, 370, 490, 526, 911, 1087
Hpy188III TCNNGA 9 cut(s) 80, 117, 323, 531, 615, 737, 1067, 1219, 1303
Hpy8I GTNNAC 1 cut(s) 1397
HpyAV CCTTC 6 cut(s) 399, 663, 776, 1132, 1213, 1337
HpyCH4III ACNGT 7 cut(s) 274, 366, 402, 955, 1249, 1506, 1608
HpyCH4IV ACGT 2 cut(s) 649, 891
HpyF10VI GCNNNNNNNGC 7 cut(s) 418, 689, 1394, 1432, 1465, 1543, 1558
HpyF3I CTNAG 5 cut(s) 21, 258, 489, 568, 1273
HpySE526I ACGT 2 cut(s) 649, 891
Hsp92I GRCGYC 1 cut(s) 891
Hsp92II CATG 6 cut(s) 83, 625, 1048, 1388, 1412, 1526
Ksp22I TGATCA 2 cut(s) 64, 1525
Kzo9I GATC 6 cut(s) 64, 261, 624, 1221, 1525, 1618
LguI GCTCTTC 1 cut(s) 1458
LmnI GCTCC 5 cut(s) 982, 1069, 1179, 1570, 1591
Lsp1109I GCAGC 5 cut(s) 800, 822, 1435, 1438, 1570
LweI GCATC 4 cut(s) 556, 608, 1411, 1582
MaeI CTAG 8 cut(s) 117, 222, 243, 531, 827, 1389, 1638, 1646
MaeII ACGT 2 cut(s) 649, 891
MaeIII GTNAC 3 cut(s) 463, 887, 922
MalI GATC 6 cut(s) 66, 263, 626, 1223, 1527, 1620
MbiI CCGCTC 1 cut(s) 561
MboI GATC 6 cut(s) 64, 261, 624, 1221, 1525, 1618
MflI RGATCY 1 cut(s) 1618
MhlI GDGCHC 7 cut(s) 59, 777, 1097, 1105, 1399, 1596, 1664
MluCI AATT 7 cut(s) 10, 189, 313, 534, 633, 1266, 1321
MlyI GAGTC 2 cut(s) 442, 526
MmeI TCCRAC 3 cut(s) 211, 360, 1590
Mph1103I ATGCAT 1 cut(s) 623
MroXI GAANNNNTTC 1 cut(s) 732
MseI TTAA 5 cut(s) 543, 599, 798, 870, 1254
MslI CAYNNNNRTG 2 cut(s) 1169, 1407
MspA1I CMGCKG 1 cut(s) 813
MspI CCGG 5 cut(s) 149, 304, 353, 1025, 1432
MspR9I CCNGG 2 cut(s) 305, 1037
MvaI CCWGG 1 cut(s) 1037
MwoI GCNNNNNNNGC 7 cut(s) 418, 689, 1394, 1432, 1465, 1543, 1558
NciI CCSGG 1 cut(s) 305
NdeI CATATG 2 cut(s) 552, 1187
NdeII GATC 6 cut(s) 64, 261, 624, 1221, 1525, 1618
NheI GCTAGC 2 cut(s) 826, 1388
NlaIII CATG 6 cut(s) 83, 625, 1048, 1388, 1412, 1526
NlaIV GGNNCC 1 cut(s) 1095
NmuCI GTSAC 1 cut(s) 887
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 2 cut(s) 1048, 1388
OliI CACNNNNGTG 1 cut(s) 1169
PaeI GCATGC 1 cut(s) 1048
PagI TCATGA 1 cut(s) 79
PciSI GCTCTTC 1 cut(s) 1458
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 8 cut(s) 41, 76, 340, 527, 611, 719, 791, 1517
PflMI CCANNNNNTGG 1 cut(s) 1609
PinAI ACCGGT 1 cut(s) 1024
PkrI GCNGC 6 cut(s) 812, 815, 1425, 1428, 1560, 1644
PleI GAGTC 2 cut(s) 442, 525
PpsI GAGTC 2 cut(s) 442, 525
Psp124BI GAGCTC 2 cut(s) 59, 777
Psp6I CCWGG 1 cut(s) 1035
PspFI CCCAGC 2 cut(s) 688, 1485
PspGI CCWGG 1 cut(s) 1035
PspN4I GGNNCC 1 cut(s) 1095
PspOMI GGGCCC 1 cut(s) 1093
PspPI GGNCC 2 cut(s) 1093, 1094
PstI CTGCAG 1 cut(s) 1425
PstNI CAGNNNCTG 3 cut(s) 20, 713, 1011
PsuI RGATCY 1 cut(s) 1618
PvuII CAGCTG 1 cut(s) 813
RsaI GTAC 4 cut(s) 184, 1251, 1532, 1542
RsaNI GTAC 4 cut(s) 183, 1250, 1531, 1541
RseI CAYNNNNRTG 2 cut(s) 1169, 1407
SacI GAGCTC 2 cut(s) 59, 777
SapI GCTCTTC 1 cut(s) 1458
SaqAI TTAA 5 cut(s) 543, 599, 798, 870, 1254
SatI GCNGC 6 cut(s) 811, 814, 1424, 1427, 1559, 1643
Sau3AI GATC 6 cut(s) 64, 261, 624, 1221, 1525, 1618
Sau96I GGNCC 2 cut(s) 1093, 1094
SchI GAGTC 2 cut(s) 442, 526
ScrFI CCNGG 2 cut(s) 305, 1037
SduI GDGCHC 7 cut(s) 59, 777, 1097, 1105, 1399, 1596, 1664
SfaNI GCATC 4 cut(s) 556, 608, 1411, 1582
SfcI CTRYAG 2 cut(s) 1421, 1454
SmiMI CAYNNNNRTG 2 cut(s) 1169, 1407
SphI GCATGC 1 cut(s) 1048
Sse9I AATT 7 cut(s) 10, 189, 313, 534, 633, 1266, 1321
SsiI CCGC 5 cut(s) 5, 239, 559, 604, 1643
SspI AATATT 1 cut(s) 583
SspMI CTAG 8 cut(s) 117, 222, 243, 531, 827, 1389, 1638, 1646
SstI GAGCTC 2 cut(s) 59, 777
StyD4I CCNGG 2 cut(s) 303, 1035
StyI CCWWGG 1 cut(s) 242
TaaI ACNGT 7 cut(s) 274, 366, 402, 955, 1249, 1506, 1608
TaiI ACGT 2 cut(s) 652, 894
TaqI TCGA 1 cut(s) 1579
TasI AATT 7 cut(s) 10, 189, 313, 534, 633, 1266, 1321
TatI WGTACW 2 cut(s) 1249, 1540
TauI GCSGC 1 cut(s) 1645
TfiI GAWTC 8 cut(s) 41, 76, 340, 527, 611, 719, 791, 1517
Tru1I TTAA 5 cut(s) 543, 599, 798, 870, 1254
Tru9I TTAA 5 cut(s) 543, 599, 798, 870, 1254
TscAI CASTG 6 cut(s) 699, 718, 1300, 1399, 1404, 1454
TseFI GTSAC 1 cut(s) 887
TseI GCWGC 5 cut(s) 810, 813, 1423, 1426, 1558
Tsp45I GTSAC 1 cut(s) 887
TspDTI ATGAA 5 cut(s) 68, 96, 114, 1137, 1425
TspGWI ACGGA 3 cut(s) 65, 366, 617
TspRI CASTG 6 cut(s) 699, 718, 1300, 1399, 1404, 1454
Van91I CCANNNNNTGG 1 cut(s) 1609
VneI GTGCAC 1 cut(s) 1395
XapI RAATTY 2 cut(s) 313, 1321
XbaI TCTAGA 2 cut(s) 116, 530
XceI RCATGY 2 cut(s) 1048, 1388
XcmI CCANNNNNNNNNTGG 1 cut(s) 1610
XmaJI CCTAGG 1 cut(s) 242
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 8 cut(s) 117, 222, 243, 531, 827, 1389, 1638, 1646
ZraI GACGTC 1 cut(s) 892
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.