Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0009530

Physical Location & Seq

Genomic Coordinates Reverse (-)

9304016 .. 9307084

Gene Structure 3069 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2361 bp

ATGGAGGGAAAAGTATATAAGTTTCTCCATGGAACTCTTGAAGCTACCATCTTCAATGCCACACCTTACTCACCACCACCAATATTCCCCTTCAATTGTATTTTGGCAAATGGAAAGCCTGCATATGTGACCATCAAAATAGACAACAAGAAAGTAGCAAAGACGACTCACGAACGCGACCGTGTGTGGAACCAAACCTTTCAAATCCTCTGTGCTCATCCGCCTGATTCGACCATCACCATTACCATGAAAACAAAATGCTCCATCTTGGGAAAATTTCAAATTCAGGCACATGAGATACTGAACGAAGCAAGTTTTATCAACGGTTTTCTCCCTCTTGTAATCGAAAATGGGAAGCCAAATCCAGAACTCAAGCTCCGGTTTATGTTATGGTTTAAGCCCGTGAAGTTTGAACCAACTTGGGGGACAATAACTGATAATGGAGGATTCAAGGGGCTGAGCAATGCATCATTTCCACAACGATCCAATAGCCATGTCACCCTTTATCAAGATGCTCACCACTGCTCAACTTTCAACCCTACGCCAGAGTTTTGTGGGAGTCGGAGAAGACTATGGGAAGATGTATACAAAGCCATTGAGGGGGCAACACATTTGATTTACATTGCGGGCTGGTCTTTCAATCCTAAGATGGTTCTGGTAAATAGAATCCCAACACAATTTTCCAAGGATCCTCAAACAGACATTCCGCATGCGCGAGGGGTGAAGCTCGGTGAATTACTAAAGCAGAAGGCCGAGGAAGGTGTGGCAGTGAGAATAATGCTTTGGGATGATCAAACTTCATTAAAAATTATCAAGAACAAAGGAATAATGGGAACACATGATGAAGATGCATTTTGCTACTTTGCACACACGAAAGTGATATGCAAATTGTGCCCTAGATTGCACCGCAAGTTCCCCACAATCTTCTCTCACCATCAGAAGACCATAACTGTAGACATCAAATCCCTCACCAGTGCAAGTGACAAAGAAATCATGAGCTTTGTTGGGGGTCTAGATCTTTGTGATGGGCGCTACGACACGGAGCAACATTCGTTGTTTCAAACACTCAATACAGAATCACATTGTGCTGATTTCTACCAGACCAACATTTCCGGGGCTAGCCTTCAGAGAGGAGGGCCAAGAACGCCGTGGCATGATGCTCACGCTTGTATAACAGGCGAAGCTGCTTGGGATGTTCTAACAAATTTCGAGCAACGATGGTCTAAGAAATGTGATCGATCTTTATTGGTCCCTTCTATCACCTTAACAAGCCTCATTCAACAGACTTATGCAAGTAAACCCTCTGAGAGGGGCTGGAATGTTCAAGTTCTTAGGTCAATTGACAATGTTTCGGCAAGCCAATTGTTTCGAAACCTGGCCGTGGAACATAGCATTCATGAAGCCTACGTTGAAGCAATTAGGCGCGCCGATAAGTTTCTATACATCGAGAACCAGTATTTCATGGGAGGGTGCTATTTGTGGGAGAACGATCGGCATAGTGGATGTAGAAACTTGATCCCCGTTGAAATTGCACTAAAAGTGGTCAGTAAGATCAAAGAAAAGGAGAGGTTTGCAGTGTATATCGTGATTCCAATGTGGCCAGAAGGGTTACCTGAGAGTGAACCTGTGCAGGATATACTTCATTGGACTAGAGAGACAATGTCAATGATGTATAGATTGATTGGAGAAGCCATAAAAGAAAGTGGAGTTGAAGGGCATCACCCAAGAGACTATTTGAACTTCTATTGCCTTGCCAATAGAGAAGTTGAGACCCAAGGGGAGTTTGTTCCCCCTCATTCTCCTCATCATGCAACACAATATTGGAATGCGCAAAAGCATAGGAGGTTCATGGTCTATGTTCACTCCAAGCTCATGATAGTAATTTCTCTTTGTTGTGGTGCCTACTATGATCTAGTGGACGATGCATACCTTATCATAGGATCTGCAAACATAAACCAAAGATCCATGGACGGACATCGAGACACTGAGATCGCCATTGGATGCTACCAGTCCGACAACAGTGACAACAAGACGATGGTGAGCTCAGGAGGCAACATTGGAGCATACCGAATGTCATTATGGTACGAACACACAGGTCGTTTCGAAGAACTGTTCAAGCAACCCGAAAGCTTAGAATGCGTGCAGAAGATGCGTTTTATCGGAGACCGGATGTGGGAAATTTACAGTGGGGAAGAAGTGGTCGACATGGAAGGTGTTCATCTTGTGACATATCCCATGAGTGTTTCAGAGGAAGGATATGTTGAGGACCTTGTTAAAAGTAGTGATGGTCATTTTCCAGATACAAAGGCTATGGTGAAGGGCAAGAGATCAAAAGTGATACCATCAATATTTACTACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0005575 GO:0005623 GO:0005886 GO:0006629 GO:0006644 GO:0006793 GO:0006796 GO:0006808 GO:0006950 GO:0006970 GO:0006995 GO:0007154 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0009056 GO:0009267 GO:0009395 GO:0009605 GO:0009628 GO:0009791 GO:0009825 GO:0009987 GO:0009991 GO:0016020 GO:0016036 GO:0016042 GO:0016049 GO:0016298 GO:0016787 GO:0016788 GO:0019637 GO:0022622 GO:0031667 GO:0031668 GO:0031669 GO:0032501 GO:0032502 GO:0033554 GO:0040007 GO:0042578 GO:0042594 GO:0043562 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044464 GO:0045848 GO:0046434 GO:0048364 GO:0048518 GO:0048731 GO:0048856 GO:0050789 GO:0050896 GO:0051301 GO:0051365 GO:0051716 GO:0065007 GO:0071496 GO:0071704 GO:0071944 GO:0099402 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

787

Amino Acids

89.77

Weight (kDa)

6.91

Isoelectric Point (pI)

37.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PLD_C	PF12357	702 - 775	1.6e-23	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55180 AT1G55180
fragaria_vesca	FvH4_6g33140
malus_domestica	MD09G1194700.v1.1 MD17G1175200.v1.1
prunus_persica	Prupe.3G051200_v2.0.a1
pyrus_communis	pycom09g11160
rosa_chinensis	RchiOBHm_Chr2g0142831
rosa_laevigata	RLG00000019989
rosa_multiflora	Rmu_co8241083.1_g000001 Rmu_co8446503.1_g000001 Rmu_sc0012306.1_g000009 Rmu_sc0013116.1_g000004
rosa_roxburghii	Rroxscaffold_2G00102280 Rroxscaffold_2G00103320
rosa_rugosa	Rorug02G0375600 Rorug02G0375700
rosa_samantha	Rh2AG428800 Rh2BG438100 Rh2CG414300 Rh2DG447900
rosa_wichuraiana	Rw2G035060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1830
AccB1I	GGYRCC	1 cut(s)	1898
AccI	GTMKAC	3 cut(s)	585, 954, 2202
AccII	CGCG	3 cut(s)	177, 715, 1425
AciI	CCGC	4 cut(s)	221, 626, 707, 907
AclWI	GGATC	6 cut(s)	477, 683, 696, 1510, 1948, 1956
AcoI	YGGCCR	2 cut(s)	1377, 1598
AcsI	RAATTY	4 cut(s)	275, 282, 1204, 2178
AcuI	CTGAAG	1 cut(s)	1109
AdeI	CACNNNGTG	1 cut(s)	1085
AfaI	GTAC	1 cut(s)	2084
AfiI	CCNNNNNNNGG	5 cut(s)	422, 600, 1308, 1381, 2173
AjnI	CCWGG	1 cut(s)	1374
AjuI	GAANNNNNNNTTGG	4 cut(s)	664, 696, 766, 798
AleI	CACNNNNGTG	1 cut(s)	875
AloI	GAACNNNNNNTCC	2 cut(s)	360, 392
AluBI	AGCT	8 cut(s)	44, 376, 727, 999, 1184, 1870, 2043, 2130
AluI	AGCT	8 cut(s)	44, 376, 727, 999, 1184, 1870, 2043, 2130
Alw21I	GWGCWC	2 cut(s)	217, 2045
Alw26I	GTCTC	5 cut(s)	1649, 1722, 1763, 1974, 2157
AlwI	GGATC	6 cut(s)	477, 683, 696, 1510, 1948, 1956
AoxI	GGCC	4 cut(s)	750, 1136, 1377, 1598
ApeKI	GCWGC	1 cut(s)	1184
ApoI	RAATTY	4 cut(s)	275, 282, 1204, 2178
AscI	GGCGCGCC	1 cut(s)	1423
Asp700I	GAANNNNTTC	1 cut(s)	2214
AspLEI	GCGC	5 cut(s)	715, 1032, 1425, 1427, 1831
AspS9I	GGNCC	3 cut(s)	1136, 1249, 2266
AsuC2I	CCSGG	1 cut(s)	1114
AsuII	TTCGAA	2 cut(s)	1369, 2103
AsuNHI	GCTAGC	1 cut(s)	1118
AvaII	GGWCC	2 cut(s)	1249, 2266
BaeGI	GKGCMC	1 cut(s)	896
BalI	TGGCCA	1 cut(s)	1600
BamHI	GGATCC	1 cut(s)	688
BanI	GGYRCC	1 cut(s)	1898
BanII	GRGCYC	1 cut(s)	2045
BbsI	GAAGAC	2 cut(s)	574, 947
Bbv12I	GWGCWC	2 cut(s)	217, 2045
BbvI	GCAGC	1 cut(s)	1171
BceAI	ACGGC	2 cut(s)	1132, 1364
BciT130I	CCWGG	1 cut(s)	1376
BclI	TGATCA	1 cut(s)	790
BcnI	CCSGG	1 cut(s)	1114
BcoDI	GTCTC	5 cut(s)	1649, 1722, 1763, 1974, 2157
BfaI	CTAG	5 cut(s)	897, 1013, 1119, 1650, 1913
BfmI	CTRYAG	1 cut(s)	951
BfoI	RGCGCY	1 cut(s)	1033
BglII	AGATCT	1 cut(s)	1015
BisI	GCNGC	1 cut(s)	1185
BlpI	GCTNAGC	1 cut(s)	458
BlsI	GCNGC	1 cut(s)	1186
Bme1390I	CCNGG	2 cut(s)	1114, 1376
Bme18I	GGWCC	2 cut(s)	1249, 2266
BmgT120I	GGNCC	3 cut(s)	1136, 1249, 2266
BmiI	GGNNCC	4 cut(s)	191, 690, 1251, 1900
BmrFI	CCNGG	2 cut(s)	1114, 1376
BmsI	GCATC	8 cut(s)	476, 502, 838, 1147, 1726, 1912, 1991, 2139
BmtI	GCTAGC	1 cut(s)	1122
BpiI	GAAGAC	2 cut(s)	574, 947
Bpu10I	CCTNAGC	1 cut(s)	2044
Bpu1102I	GCTNAGC	1 cut(s)	458
Bpu14I	TTCGAA	2 cut(s)	1369, 2103
BpuEI	CTTGAG	1 cut(s)	356
BpuMI	CCSGG	1 cut(s)	1114
Bsa29I	ATCGAT	1 cut(s)	1237
BsaI	GGTCTC	2 cut(s)	1763, 2157
BsaJI	CCNNGG	8 cut(s)	28, 684, 753, 1113, 1148, 1380, 1774, 1965
BsaWI	WCCGGW	2 cut(s)	378, 2166
BsaXI	ACNNNNNCTCC	2 cut(s)	360, 390
Bsc4I	CCNNNNNNNGG	5 cut(s)	422, 600, 1308, 1381, 2173
Bse1I	ACTGG	3 cut(s)	972, 1453, 2008
Bse3DI	GCAATG	2 cut(s)	469, 621
BseBI	CCWGG	1 cut(s)	1376
BseCI	ATCGAT	1 cut(s)	1237
BseDI	CCNNGG	8 cut(s)	28, 684, 753, 1113, 1148, 1380, 1774, 1965
BseGI	GGATG	6 cut(s)	217, 793, 1198, 1508, 2006, 2175
BseLI	CCNNNNNNNGG	5 cut(s)	422, 600, 1308, 1381, 2173
BseMI	GCAATG	2 cut(s)	469, 621
BseMII	CTCAG	5 cut(s)	449, 1296, 1605, 1977, 2058
BseNI	ACTGG	3 cut(s)	972, 1453, 2008
BsePI	GCGCGC	1 cut(s)	1423
BseRI	GAGGAG	2 cut(s)	1146, 1791
BseSI	GKGCMC	1 cut(s)	896
BseXI	GCAGC	1 cut(s)	1171
BsgI	GTGCAG	2 cut(s)	1649, 2162
Bsh1236I	CGCG	3 cut(s)	177, 715, 1425
Bsh1285I	CGRYCG	2 cut(s)	181, 1492
BshFI	GGCC	4 cut(s)	752, 1138, 1379, 1600
BshNI	GGYRCC	1 cut(s)	1898
BshVI	ATCGAT	1 cut(s)	1237
BsiEI	CGRYCG	2 cut(s)	181, 1492
BsiHKAI	GWGCWC	2 cut(s)	217, 2045
BsiSI	CCGG	3 cut(s)	379, 1113, 2167
BslFI	GGGAC	2 cut(s)	439, 1235
BslI	CCNNNNNNNGG	5 cut(s)	422, 600, 1308, 1381, 2173
BsmAI	GTCTC	5 cut(s)	1649, 1722, 1763, 1974, 2157
BsmFI	GGGAC	2 cut(s)	439, 1235
BsmI	GAATGC	3 cut(s)	1392, 1831, 2141
BsnI	GGCC	4 cut(s)	752, 1138, 1379, 1600
Bso31I	GGTCTC	2 cut(s)	1763, 2157
Bsp119I	TTCGAA	2 cut(s)	1369, 2103
Bsp1286I	GDGCHC	3 cut(s)	217, 896, 2045
Bsp1720I	GCTNAGC	1 cut(s)	458
Bsp19I	CCATGG	2 cut(s)	28, 1965
BspACI	CCGC	4 cut(s)	221, 626, 707, 907
BspANI	GGCC	4 cut(s)	752, 1138, 1379, 1600
BspCNI	CTCAG	5 cut(s)	450, 1297, 1606, 1978, 2057
BspDI	ATCGAT	1 cut(s)	1237
BspFNI	CGCG	3 cut(s)	177, 715, 1425
BspHI	TCATGA	3 cut(s)	993, 1396, 1872
BspLI	GGNNCC	4 cut(s)	191, 690, 1251, 1900
BspOI	GCTAGC	1 cut(s)	1122
BspPI	GGATC	6 cut(s)	477, 683, 696, 1510, 1948, 1956
BspT104I	TTCGAA	2 cut(s)	1369, 2103
BspT107I	GGYRCC	1 cut(s)	1898
BspTNI	GGTCTC	2 cut(s)	1763, 2157
BsrDI	GCAATG	2 cut(s)	469, 621
BsrI	ACTGG	3 cut(s)	972, 1453, 2008
BssECI	CCNNGG	8 cut(s)	28, 684, 753, 1113, 1148, 1380, 1774, 1965
BssHII	GCGCGC	1 cut(s)	1423
BssNAI	GTATAC	1 cut(s)	586
BssT1I	CCWWGG	4 cut(s)	28, 684, 1774, 1965
Bst1107I	GTATAC	1 cut(s)	586
Bst2UI	CCWGG	1 cut(s)	1376
Bst4CI	ACNGT	6 cut(s)	182, 326, 952, 2021, 2112, 2186
BstAPI	GCANNNNNTGC	2 cut(s)	891, 2149
BstBI	TTCGAA	2 cut(s)	1369, 2103
BstC8I	GCNNGC	7 cut(s)	120, 628, 711, 1120, 1357, 1425, 2141
BstDEI	CTNAG	9 cut(s)	458, 645, 1224, 1305, 1331, 1614, 1986, 2044, 2131
BstDSI	CCRYGG	4 cut(s)	28, 1148, 1380, 1965
BstEII	GGTNACC	1 cut(s)	1608
BstENI	CCTNNNNNAGG	1 cut(s)	1306
BstF5I	GGATG	6 cut(s)	217, 793, 1198, 1508, 2006, 2175
BstFNI	CGCG	3 cut(s)	177, 715, 1425
BstH2I	RGCGCY	1 cut(s)	1033
BstHHI	GCGC	5 cut(s)	715, 1032, 1425, 1427, 1831
BstMAI	GTCTC	5 cut(s)	1649, 1722, 1763, 1974, 2157
BstMCI	CGRYCG	2 cut(s)	181, 1492
BstMWI	GCNNNNNNNGC	5 cut(s)	891, 1144, 2049, 2136, 2149
BstNI	CCWGG	1 cut(s)	1376
BstNSI	RCATGY	1 cut(s)	713
BstPI	GGTNACC	1 cut(s)	1608
BstSCI	CCNGG	2 cut(s)	1112, 1374
BstSFI	CTRYAG	1 cut(s)	951
BstSLI	GKGCMC	1 cut(s)	896
BstUI	CGCG	3 cut(s)	177, 715, 1425
BstV1I	GCAGC	1 cut(s)	1171
BstV2I	GAAGAC	2 cut(s)	574, 947
BstX2I	RGATCY	4 cut(s)	688, 1015, 1940, 1961
BstYI	RGATCY	4 cut(s)	688, 1015, 1940, 1961
BstZ17I	GTATAC	1 cut(s)	586
Bsu15I	ATCGAT	1 cut(s)	1237
BsuRI	GGCC	4 cut(s)	752, 1138, 1379, 1600
BsuTUI	ATCGAT	1 cut(s)	1237
BtgI	CCRYGG	4 cut(s)	28, 1148, 1380, 1965
BtsCI	GGATG	6 cut(s)	217, 793, 1198, 1508, 2006, 2175
BtsI	GCAGTG	3 cut(s)	520, 774, 1581
BtsIMutI	CAGTG	7 cut(s)	520, 774, 979, 1581, 1983, 2026, 2191
Cac8I	GCNNGC	7 cut(s)	120, 628, 711, 1120, 1357, 1425, 2141
CciI	TCATGA	3 cut(s)	993, 1396, 1872
CfoI	GCGC	5 cut(s)	715, 1032, 1425, 1427, 1831
Cfr13I	GGNCC	3 cut(s)	1136, 1249, 2266
ClaI	ATCGAT	1 cut(s)	1237
Csp6I	GTAC	1 cut(s)	2083
CviQI	GTAC	1 cut(s)	2083
DdeI	CTNAG	9 cut(s)	458, 645, 1224, 1305, 1331, 1614, 1986, 2044, 2131
DraIII	CACNNNGTG	1 cut(s)	1085
EaeI	YGGCCR	2 cut(s)	1377, 1598
EciI	GGCGGA	1 cut(s)	210
Ecl136II	GAGCTC	1 cut(s)	2043
Eco130I	CCWWGG	4 cut(s)	28, 684, 1774, 1965
Eco24I	GRGCYC	1 cut(s)	2045
Eco31I	GGTCTC	2 cut(s)	1763, 2157
Eco47I	GGWCC	2 cut(s)	1249, 2266
Eco53kI	GAGCTC	1 cut(s)	2043
Eco57I	CTGAAG	1 cut(s)	1109
Eco91I	GGTNACC	1 cut(s)	1608
EcoICRI	GAGCTC	1 cut(s)	2043
EcoNI	CCTNNNNNAGG	1 cut(s)	1306
EcoO109I	RGGNCCY	1 cut(s)	2266
EcoO65I	GGTNACC	1 cut(s)	1608
EcoRII	CCWGG	1 cut(s)	1374
EcoT14I	CCWWGG	4 cut(s)	28, 684, 1774, 1965
EcoT22I	ATGCAT	3 cut(s)	469, 853, 1927
EcoT38I	GRGCYC	1 cut(s)	2045
ErhI	CCWWGG	4 cut(s)	28, 684, 1774, 1965
FaqI	GGGAC	2 cut(s)	439, 1235
FauI	CCCGC	1 cut(s)	619
FauNDI	CATATG	1 cut(s)	124
FbaI	TGATCA	1 cut(s)	790
FblI	GTMKAC	3 cut(s)	585, 954, 2202
Fnu4HI	GCNGC	1 cut(s)	1185
FokI	GGATG	6 cut(s)	204, 800, 1205, 1515, 2013, 2182
FriOI	GRGCYC	1 cut(s)	2045
Fsp4HI	GCNGC	1 cut(s)	1185
FspBI	CTAG	5 cut(s)	897, 1013, 1119, 1650, 1913
FspI	TGCGCA	1 cut(s)	1830
GlaI	GCGC	5 cut(s)	714, 1031, 1424, 1426, 1830
GluI	GCNGC	1 cut(s)	1185
HaeII	RGCGCY	1 cut(s)	1033
HaeIII	GGCC	4 cut(s)	752, 1138, 1379, 1600
HapII	CCGG	3 cut(s)	379, 1113, 2167
HhaI	GCGC	5 cut(s)	715, 1032, 1425, 1427, 1831
Hin6I	GCGC	5 cut(s)	713, 1030, 1423, 1425, 1829
HinP1I	GCGC	5 cut(s)	713, 1030, 1423, 1425, 1829
HincII	GTYRAC	1 cut(s)	2203
HindII	GTYRAC	1 cut(s)	2203
HindIII	AAGCTT	1 cut(s)	2128
HinfI	GANTC	7 cut(s)	166, 227, 447, 559, 666, 1076, 1588
HpaII	CCGG	3 cut(s)	379, 1113, 2167
Hpy166II	GTNNAC	7 cut(s)	586, 955, 1298, 1622, 1861, 1918, 2203
Hpy188I	TCNGA	7 cut(s)	564, 939, 1128, 1306, 2014, 2162, 2248
Hpy8I	GTNNAC	7 cut(s)	586, 955, 1298, 1622, 1861, 1918, 2203
HpyCH4III	ACNGT	6 cut(s)	182, 326, 952, 2021, 2112, 2186
HpyCH4IV	ACGT	1 cut(s)	1407
HpyF10VI	GCNNNNNNNGC	5 cut(s)	891, 1144, 2049, 2136, 2149
HpyF3I	CTNAG	9 cut(s)	458, 645, 1224, 1305, 1331, 1614, 1986, 2044, 2131
HpySE526I	ACGT	1 cut(s)	1407
HspAI	GCGC	5 cut(s)	713, 1030, 1423, 1425, 1829
Ksp22I	TGATCA	1 cut(s)	790
LmnI	GCTCC	4 cut(s)	266, 381, 1042, 2060
Lsp1109I	GCAGC	1 cut(s)	1171
LweI	GCATC	8 cut(s)	476, 502, 838, 1147, 1726, 1912, 1991, 2139
MaeI	CTAG	5 cut(s)	897, 1013, 1119, 1650, 1913
MaeII	ACGT	1 cut(s)	1407
MaeIII	GTNAC	6 cut(s)	127, 496, 980, 1608, 2021, 2224
MboII	GAAGA	9 cut(s)	43, 579, 590, 857, 916, 952, 2117, 2158, 2204
MfeI	CAATTG	3 cut(s)	94, 1338, 1361
MflI	RGATCY	4 cut(s)	688, 1015, 1940, 1961
MhlI	GDGCHC	3 cut(s)	217, 896, 2045
MlsI	TGGCCA	1 cut(s)	1600
MluNI	TGGCCA	1 cut(s)	1600
MlyI	GAGTC	2 cut(s)	160, 568
MmeI	TCCRAC	2 cut(s)	542, 2037
Mox20I	TGGCCA	1 cut(s)	1600
Mph1103I	ATGCAT	3 cut(s)	469, 853, 1927
MroXI	GAANNNNTTC	1 cut(s)	2214
MscI	TGGCCA	1 cut(s)	1600
MseI	TTAA	4 cut(s)	396, 803, 1265, 2274
MslI	CAYNNNNRTG	2 cut(s)	245, 875
Msp20I	TGGCCA	1 cut(s)	1600
MspI	CCGG	3 cut(s)	379, 1113, 2167
MspR9I	CCNGG	2 cut(s)	1114, 1376
MunI	CAATTG	3 cut(s)	94, 1338, 1361
Mva1269I	GAATGC	3 cut(s)	1392, 1831, 2141
MvaI	CCWGG	1 cut(s)	1376
MvnI	CGCG	3 cut(s)	177, 715, 1425
MwoI	GCNNNNNNNGC	5 cut(s)	891, 1144, 2049, 2136, 2149
NciI	CCSGG	1 cut(s)	1114
NcoI	CCATGG	2 cut(s)	28, 1965
NdeI	CATATG	1 cut(s)	124
NheI	GCTAGC	1 cut(s)	1118
NlaIV	GGNNCC	4 cut(s)	191, 690, 1251, 1900
NmeAIII	GCCGAG	1 cut(s)	778
NmuCI	GTSAC	5 cut(s)	127, 496, 980, 2021, 2224
NsbI	TGCGCA	1 cut(s)	1830
NsiI	ATGCAT	3 cut(s)	469, 853, 1927
NspI	RCATGY	1 cut(s)	713
NspV	TTCGAA	2 cut(s)	1369, 2103
OliI	CACNNNNGTG	1 cut(s)	875
PaeI	GCATGC	1 cut(s)	713
PagI	TCATGA	3 cut(s)	993, 1396, 1872
PalAI	GGCGCGCC	1 cut(s)	1423
PauI	GCGCGC	1 cut(s)	1423
PctI	GAATGC	3 cut(s)	1392, 1831, 2141
PdmI	GAANNNNTTC	1 cut(s)	2214
PfeI	GAWTC	5 cut(s)	227, 447, 666, 1076, 1588
PflFI	GACNNNGTC	1 cut(s)	1660
PkrI	GCNGC	1 cut(s)	1186
Ple19I	CGATCG	1 cut(s)	1492
PleI	GAGTC	2 cut(s)	160, 567
PpsI	GAGTC	2 cut(s)	160, 567
PpuMI	RGGWCCY	1 cut(s)	2266
Psp124BI	GAGCTC	1 cut(s)	2045
Psp5II	RGGWCCY	1 cut(s)	2266
Psp6I	CCWGG	1 cut(s)	1374
PspEI	GGTNACC	1 cut(s)	1608
PspGI	CCWGG	1 cut(s)	1374
PspN4I	GGNNCC	4 cut(s)	191, 690, 1251, 1900
PspPI	GGNCC	3 cut(s)	1136, 1249, 2266
PspPPI	RGGWCCY	1 cut(s)	2266
PsuI	RGATCY	4 cut(s)	688, 1015, 1940, 1961
PsyI	GACNNNGTC	1 cut(s)	1660
PteI	GCGCGC	1 cut(s)	1423
PvuI	CGATCG	1 cut(s)	1492
RsaI	GTAC	1 cut(s)	2084
RsaNI	GTAC	1 cut(s)	2083
RseI	CAYNNNNRTG	2 cut(s)	245, 875
SacI	GAGCTC	1 cut(s)	2045
SalI	GTCGAC	1 cut(s)	2201
SaqAI	TTAA	4 cut(s)	396, 803, 1265, 2274
SatI	GCNGC	1 cut(s)	1185
Sau96I	GGNCC	3 cut(s)	1136, 1249, 2266
SchI	GAGTC	2 cut(s)	160, 568
ScrFI	CCNGG	2 cut(s)	1114, 1376
SduI	GDGCHC	3 cut(s)	217, 896, 2045
SfaNI	GCATC	8 cut(s)	476, 502, 838, 1147, 1726, 1912, 1991, 2139
SfcI	CTRYAG	1 cut(s)	951
SfuI	TTCGAA	2 cut(s)	1369, 2103
SgsI	GGCGCGCC	1 cut(s)	1423
SinI	GGWCC	2 cut(s)	1249, 2266
SmiMI	CAYNNNNRTG	2 cut(s)	245, 875
SmlI	CTYRAG	1 cut(s)	371
SmoI	CTYRAG	1 cut(s)	371
SphI	GCATGC	1 cut(s)	713
SsiI	CCGC	4 cut(s)	221, 626, 707, 907
SspI	AATATT	3 cut(s)	84, 1820, 2349
SspMI	CTAG	5 cut(s)	897, 1013, 1119, 1650, 1913
SstI	GAGCTC	1 cut(s)	2045
StyD4I	CCNGG	2 cut(s)	1112, 1374
StyI	CCWWGG	4 cut(s)	28, 684, 1774, 1965
TaaI	ACNGT	6 cut(s)	182, 326, 952, 2021, 2112, 2186
TaiI	ACGT	1 cut(s)	1410
TaqI	TCGA	9 cut(s)	230, 345, 1209, 1237, 1369, 1446, 1978, 2103, 2202
TfiI	GAWTC	5 cut(s)	227, 447, 666, 1076, 1588
Tru1I	TTAA	4 cut(s)	396, 803, 1265, 2274
Tru9I	TTAA	4 cut(s)	396, 803, 1265, 2274
TscAI	CASTG	7 cut(s)	527, 774, 979, 1581, 1990, 2026, 2191
TseFI	GTSAC	5 cut(s)	127, 496, 980, 2021, 2224
TseI	GCWGC	1 cut(s)	1184
Tsp45I	GTSAC	5 cut(s)	127, 496, 980, 2021, 2224
TspDTI	ATGAA	9 cut(s)	263, 789, 858, 1385, 1413, 1450, 1631, 1837, 2207
TspGWI	ACGGA	2 cut(s)	1055, 1986
TspRI	CASTG	7 cut(s)	527, 774, 979, 1581, 1990, 2026, 2191
Tth111I	GACNNNGTC	1 cut(s)	1660
VpaK11BI	GGWCC	2 cut(s)	1249, 2266
XagI	CCTNNNNNAGG	1 cut(s)	1306
XapI	RAATTY	4 cut(s)	275, 282, 1204, 2178
XbaI	TCTAGA	1 cut(s)	1012
XceI	RCATGY	1 cut(s)	713
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1146
XmiI	GTMKAC	3 cut(s)	585, 954, 2202
XmnI	GAANNNNTTC	1 cut(s)	2214
XspI	CTAG	5 cut(s)	897, 1013, 1119, 1650, 1913
Zsp2I	ATGCAT	3 cut(s)	469, 853, 1927

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.