pycom09g11380

Anoctamin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0006221
Physical Location & Seq
Reverse (-)
9618443 .. 9621074
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1413 bp
ATGAGATTTATGCATACTTTGGTGCAAAGATCGATCTATTTTGCTTTCCTTGGAATGTATACAAGGTGGTTGCTCTTTCCAGCTGCATTTGGGCTTATCATGCACATGTTTGATTTTGGGTCATTGCAGTTACTAGTGCTTCCCATTTTCTTCATATGCATAATATTATGGGCTATAATGTTTTCACAGTTTTGGAAACGTAAAAATAGTGCCCTCTTAGCCAGATGGCAGATTAGTAATCCAGTTAGAGCTGACTTGGGGTATGGGCAAGGTGTGGAGTGGAGTTCCTCACCATCCGTTGAGCTCGCAAAAAAATGGGGGACCGACAAAACAAAAGAAAAAGAAATGTTTCAAAGAATTGAGTGGTTTGGACGACTCATGAGATTCCGAAATGATGCTATCATTATCTTCAGTATTATATGCCTTCAGTTACCATTTGAACTGGCATATGCTCATATTTATGAGGTTATGGAGTCTGATATTATGAAGGTTGGGTTGACTACCATTTATCTTTTTGCCATTCAGTTCTTGACACAGATTGGTGGCAAGATCTCCGTTAAGCTCATTAAATATGAAAATAATGAAAACACAGAAAAAAGAGCTGACAGCTTGGTCTACAAGGTGTTTGGTCTTTATTTCATGCAGTCATATATAGGAGTTTTCTACCATGCCCTTTTGCACCGCAACTTTTCTACTCTCCGCAAAGTCTTGATTCAACGCCTCCTTGTGTCTCAGGTGTTGGAAAACTTGTTGGAGAACACTTTGCCATATTTGAAGTACAGTTATAAGAAATATAGAGTTCGAAGCAAGAAAAAACGTGAAAAGGGTTCATCAATAGGGAAGATCCAGTTTGCTTCCAGGGTAGAAAAGGAGTACCTGAAGCCTTCTTATTCTGCAAGCATTGGTATGGAACTTGAAGACGGGCTGTTTGATGACTTTCTGGAGGTGGCATTGCAGTTTGGAATGATCATGATGTTTGCTTGTGCATTCCCCCTTGCTTTTGCCTTCGCAACTTTGAACAATATCACTGAAATTAGGGCTGATGCGTTGAAGCTTCTTGCCATGTTGAGAAGACCAACTCCTCGATTTTCCACAACAATTGGAGCTTGGCTGAACATATTTCAGTTTCTCATAGTTATGTCTATATGCACCAACTGTGCACTGCTGGTATGGTTATATGATGAAGAGGGCAAATGGAAGATAGAGCCTGGCCTGGCTGCAATATTGGTTATGGAACATGTCCTCTTGTTGATCAAATTTGGTTTCTCCCGTTTTGTTCCCGAGGAACCCGCTTGGGTAGAAGCCAACCGTGTGAAAAACGCAACAAATGCTGTGAACATGTGTTCTAAACAGCTCTTGAAGAGTATATCCGGAGCGAGGACGACATTTGGTGATGTTAAGAAGTCACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Anoctamin

Protein Analysis

471

Amino Acids

54.5

Weight (kDa)

9.55

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Anoctamin PF04547 11 - 438 2.3e-100 Calcium-activated chloride channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 786
AasI GACNNNNNNGTC 1 cut(s) 611
AccI GTMKAC 2 cut(s) 59, 615
AccIII TCCGGA 1 cut(s) 1370
AciI CCGC 3 cut(s) 682, 700, 1290
AclWI GGATC 1 cut(s) 838
AcsI RAATTY 1 cut(s) 1256
AcuI CTGAAG 3 cut(s) 394, 410, 899
AfaI GTAC 2 cut(s) 779, 875
AfiI CCNNNNNNNGG 1 cut(s) 1377
AflIII ACRYGT 3 cut(s) 105, 1237, 1338
AgsI TTSAA 8 cut(s) 353, 440, 716, 775, 917, 1018, 1051, 1360
AhlI ACTAGT 1 cut(s) 133
AjnI CCWGG 3 cut(s) 857, 1207, 1212
AluBI AGCT 9 cut(s) 83, 251, 304, 562, 602, 609, 1054, 1106, 1354
AluI AGCT 9 cut(s) 83, 251, 304, 562, 602, 609, 1054, 1106, 1354
Alw21I GWGCWC 2 cut(s) 306, 1162
Alw26I GTCTC 1 cut(s) 735
Alw44I GTGCAC 1 cut(s) 1158
AlwI GGATC 1 cut(s) 838
Ama87I CYCGRG 1 cut(s) 1280
Aor13HI TCCGGA 1 cut(s) 1370
AoxI GGCC 1 cut(s) 1210
ApaLI GTGCAC 1 cut(s) 1158
ApeKI GCWGC 2 cut(s) 83, 1217
ApoI RAATTY 1 cut(s) 1256
ArsI GACNNNNNNTTYG 2 cut(s) 911, 943
Asp700I GAANNNNTTC 1 cut(s) 348
AspS9I GGNCC 1 cut(s) 321
AsuHPI GGTGA 2 cut(s) 282, 1403
AsuII TTCGAA 1 cut(s) 802
AvaI CYCGRG 1 cut(s) 1280
AvaII GGWCC 1 cut(s) 321
BaeGI GKGCMC 2 cut(s) 214, 1162
BanII GRGCYC 1 cut(s) 306
BbsI GAAGAC 2 cut(s) 924, 1078
Bbv12I GWGCWC 2 cut(s) 306, 1162
BbvI GCAGC 2 cut(s) 70, 1204
BccI CCATC 2 cut(s) 219, 301
BciT130I CCWGG 3 cut(s) 859, 1209, 1214
BclI TGATCA 2 cut(s) 966, 1251
BcoDI GTCTC 1 cut(s) 735
BcuI ACTAGT 1 cut(s) 133
BfaI CTAG 1 cut(s) 134
BglII AGATCT 1 cut(s) 549
BisI GCNGC 2 cut(s) 84, 1218
BlsI GCNGC 2 cut(s) 85, 1219
Bme1390I CCNGG 3 cut(s) 859, 1209, 1214
Bme18I GGWCC 1 cut(s) 321
BmeT110I CYCGRG 1 cut(s) 1280
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 2 cut(s) 322, 1287
BmrFI CCNGG 3 cut(s) 859, 1209, 1214
BmsI GCATC 2 cut(s) 385, 1033
BpiI GAAGAC 2 cut(s) 924, 1078
BpmI CTGGAG 1 cut(s) 962
Bpu14I TTCGAA 1 cut(s) 802
Bsa29I ATCGAT 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 49, 858, 1281
BsaWI WCCGGW 1 cut(s) 1370
Bsc4I CCNNNNNNNGG 1 cut(s) 1377
Bse1I ACTGG 3 cut(s) 242, 447, 847
Bse3DI GCAATG 2 cut(s) 122, 950
BseAI TCCGGA 1 cut(s) 1370
BseBI CCWGG 3 cut(s) 859, 1209, 1214
BseCI ATCGAT 1 cut(s) 32
BseDI CCNNGG 3 cut(s) 49, 858, 1281
BseGI GGATG 1 cut(s) 293
BseLI CCNNNNNNNGG 1 cut(s) 1377
BseMI GCAATG 2 cut(s) 122, 950
BseMII CTCAG 1 cut(s) 746
BseNI ACTGG 3 cut(s) 242, 447, 847
BseRI GAGGAG 1 cut(s) 1071
BseSI GKGCMC 2 cut(s) 214, 1162
BseXI GCAGC 2 cut(s) 70, 1204
BshFI GGCC 1 cut(s) 1212
BshVI ATCGAT 1 cut(s) 32
BsiHKAI GWGCWC 2 cut(s) 306, 1162
BsiHKCI CYCGRG 1 cut(s) 1280
BsiSI CCGG 1 cut(s) 1371
BslFI GGGAC 1 cut(s) 334
BslI CCNNNNNNNGG 1 cut(s) 1377
BsmAI GTCTC 1 cut(s) 735
BsmFI GGGAC 1 cut(s) 334
BsmI GAATGC 1 cut(s) 986
BsnI GGCC 1 cut(s) 1212
BsoBI CYCGRG 1 cut(s) 1280
Bsp119I TTCGAA 1 cut(s) 802
Bsp1286I GDGCHC 3 cut(s) 214, 306, 1162
Bsp13I TCCGGA 1 cut(s) 1370
Bsp143I GATC 6 cut(s) 29, 33, 549, 843, 966, 1251
BspACI CCGC 3 cut(s) 682, 700, 1290
BspANI GGCC 1 cut(s) 1212
BspCNI CTCAG 1 cut(s) 745
BspDI ATCGAT 1 cut(s) 32
BspEI TCCGGA 1 cut(s) 1370
BspHI TCATGA 2 cut(s) 378, 969
BspLI GGNNCC 2 cut(s) 322, 1287
BspPI GGATC 1 cut(s) 838
BspT104I TTCGAA 1 cut(s) 802
BsrDI GCAATG 2 cut(s) 122, 950
BsrI ACTGG 3 cut(s) 242, 447, 847
BssECI CCNNGG 3 cut(s) 49, 858, 1281
BssMI GATC 6 cut(s) 29, 33, 549, 843, 966, 1251
BssNAI GTATAC 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 49
Bst1107I GTATAC 1 cut(s) 60
Bst2UI CCWGG 3 cut(s) 859, 1209, 1214
Bst4CI ACNGT 4 cut(s) 189, 782, 1157, 1310
Bst6I CTCTTC 2 cut(s) 1179, 1355
BstAPI GCANNNNNTGC 1 cut(s) 1328
BstBI TTCGAA 1 cut(s) 802
BstC8I GCNNGC 2 cut(s) 306, 898
BstDEI CTNAG 2 cut(s) 217, 732
BstF5I GGATG 1 cut(s) 293
BstKTI GATC 6 cut(s) 32, 36, 552, 846, 969, 1254
BstMAI GTCTC 1 cut(s) 735
BstMBI GATC 6 cut(s) 29, 33, 549, 843, 966, 1251
BstMWI GCNNNNNNNGC 3 cut(s) 100, 218, 1328
BstNI CCWGG 3 cut(s) 859, 1209, 1214
BstNSI RCATGY 3 cut(s) 109, 1241, 1342
BstSCI CCNGG 3 cut(s) 857, 1207, 1212
BstSLI GKGCMC 2 cut(s) 214, 1162
BstV1I GCAGC 2 cut(s) 70, 1204
BstV2I GAAGAC 2 cut(s) 924, 1078
BstX2I RGATCY 2 cut(s) 549, 843
BstYI RGATCY 2 cut(s) 549, 843
BstZ17I GTATAC 1 cut(s) 60
Bsu15I ATCGAT 1 cut(s) 32
BsuRI GGCC 1 cut(s) 1212
BsuTUI ATCGAT 1 cut(s) 32
BtsCI GGATG 1 cut(s) 293
BtsI GCAGTG 1 cut(s) 1160
BtsIMutI CAGTG 2 cut(s) 1026, 1160
Cac8I GCNNGC 2 cut(s) 306, 898
CciI TCATGA 2 cut(s) 378, 969
Cfr13I GGNCC 1 cut(s) 321
ClaI ATCGAT 1 cut(s) 32
Csp6I GTAC 2 cut(s) 778, 874
CspCI CAANNNNNGTGG 2 cut(s) 1081, 1116
CviAII CATG 9 cut(s) 100, 106, 379, 640, 668, 970, 1063, 1238, 1339
CviQI GTAC 2 cut(s) 778, 874
DdeI CTNAG 2 cut(s) 217, 732
DpnI GATC 6 cut(s) 31, 35, 551, 845, 968, 1253
DpnII GATC 6 cut(s) 29, 33, 549, 843, 966, 1251
DrdI GACNNNNNNGTC 1 cut(s) 611
DseDI GACNNNNNNGTC 1 cut(s) 611
Eam1104I CTCTTC 2 cut(s) 1179, 1355
EarI CTCTTC 2 cut(s) 1179, 1355
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 49
Eco24I GRGCYC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 321
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 3 cut(s) 394, 410, 899
Eco88I CYCGRG 1 cut(s) 1280
EcoICRI GAGCTC 1 cut(s) 304
EcoRII CCWGG 3 cut(s) 857, 1207, 1212
EcoT14I CCWWGG 1 cut(s) 49
EcoT22I ATGCAT 2 cut(s) 15, 161
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 9 cut(s) 103, 109, 382, 643, 671, 973, 1066, 1241, 1342
FaqI GGGAC 1 cut(s) 334
FatI CATG 9 cut(s) 99, 105, 378, 639, 667, 969, 1062, 1237, 1338
FauI CCCGC 1 cut(s) 1297
FauNDI CATATG 2 cut(s) 155, 448
FbaI TGATCA 2 cut(s) 966, 1251
FblI GTMKAC 2 cut(s) 59, 615
Fnu4HI GCNGC 2 cut(s) 84, 1218
FokI GGATG 1 cut(s) 280
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 2 cut(s) 84, 1218
FspBI CTAG 1 cut(s) 134
GluI GCNGC 2 cut(s) 84, 1218
GsuI CTGGAG 1 cut(s) 962
HaeIII GGCC 1 cut(s) 1212
HapII CCGG 1 cut(s) 1371
Hin1II CATG 9 cut(s) 103, 109, 382, 643, 671, 973, 1066, 1241, 1342
HincII GTYRAC 1 cut(s) 498
HindII GTYRAC 1 cut(s) 498
HindIII AAGCTT 1 cut(s) 1052
HinfI GANTC 4 cut(s) 375, 384, 473, 712
HpaII CCGG 1 cut(s) 1371
HphI GGTGA 2 cut(s) 282, 1403
Hpy166II GTNNAC 5 cut(s) 60, 498, 616, 1160, 1336
Hpy188I TCNGA 2 cut(s) 389, 478
Hpy188III TCNNGA 8 cut(s) 379, 529, 709, 941, 970, 1280, 1357, 1371
Hpy8I GTNNAC 5 cut(s) 60, 498, 616, 1160, 1336
HpyAV CCTTC 4 cut(s) 434, 481, 894, 1015
HpyCH4III ACNGT 4 cut(s) 189, 782, 1157, 1310
HpyCH4IV ACGT 2 cut(s) 199, 817
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 218, 1328
HpyF3I CTNAG 2 cut(s) 217, 732
HpySE526I ACGT 2 cut(s) 199, 817
Hsp92II CATG 9 cut(s) 103, 109, 382, 643, 671, 973, 1066, 1241, 1342
Kpn2I TCCGGA 1 cut(s) 1370
Ksp22I TGATCA 2 cut(s) 966, 1251
Kzo9I GATC 6 cut(s) 29, 33, 549, 843, 966, 1251
LmnI GCTCC 2 cut(s) 1103, 1373
Lsp1109I GCAGC 2 cut(s) 70, 1204
LweI GCATC 2 cut(s) 385, 1033
MaeI CTAG 1 cut(s) 134
MaeII ACGT 2 cut(s) 199, 817
MaeIII GTNAC 3 cut(s) 129, 429, 1404
MalI GATC 6 cut(s) 31, 35, 551, 845, 968, 1253
MboI GATC 6 cut(s) 29, 33, 549, 843, 966, 1251
MboII GAAGA 8 cut(s) 142, 400, 853, 929, 1083, 1196, 1210, 1372
MfeI CAATTG 1 cut(s) 1098
MflI RGATCY 2 cut(s) 549, 843
MhlI GDGCHC 3 cut(s) 214, 306, 1162
MluCI AATT 4 cut(s) 357, 1032, 1098, 1256
MlyI GAGTC 2 cut(s) 369, 482
MmeI TCCRAC 2 cut(s) 720, 732
Mph1103I ATGCAT 2 cut(s) 15, 161
MroI TCCGGA 1 cut(s) 1370
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 3 cut(s) 558, 567, 1398
MslI CAYNNNNRTG 4 cut(s) 104, 459, 905, 1136
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 1 cut(s) 1371
MspR9I CCNGG 3 cut(s) 859, 1209, 1214
MunI CAATTG 1 cut(s) 1098
Mva1269I GAATGC 1 cut(s) 986
MvaI CCWGG 3 cut(s) 859, 1209, 1214
MwoI GCNNNNNNNGC 3 cut(s) 100, 218, 1328
NdeI CATATG 2 cut(s) 155, 448
NdeII GATC 6 cut(s) 29, 33, 549, 843, 966, 1251
NlaIII CATG 9 cut(s) 103, 109, 382, 643, 671, 973, 1066, 1241, 1342
NlaIV GGNNCC 2 cut(s) 322, 1287
NmuCI GTSAC 1 cut(s) 1404
NsiI ATGCAT 2 cut(s) 15, 161
NspI RCATGY 3 cut(s) 109, 1241, 1342
NspV TTCGAA 1 cut(s) 802
PagI TCATGA 2 cut(s) 378, 969
PciI ACATGT 3 cut(s) 105, 1237, 1338
PctI GAATGC 1 cut(s) 986
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 2 cut(s) 384, 712
PkrI GCNGC 2 cut(s) 85, 1219
PleI GAGTC 2 cut(s) 369, 481
PpsI GAGTC 2 cut(s) 369, 481
PscI ACATGT 3 cut(s) 105, 1237, 1338
PsiI TTATAA 1 cut(s) 786
Psp124BI GAGCTC 1 cut(s) 306
Psp6I CCWGG 3 cut(s) 857, 1207, 1212
PspGI CCWGG 3 cut(s) 857, 1207, 1212
PspN4I GGNNCC 2 cut(s) 322, 1287
PspPI GGNCC 1 cut(s) 321
PsuI RGATCY 2 cut(s) 549, 843
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 2 cut(s) 779, 875
RsaNI GTAC 2 cut(s) 778, 874
RseI CAYNNNNRTG 4 cut(s) 104, 459, 905, 1136
SacI GAGCTC 1 cut(s) 306
SaqAI TTAA 3 cut(s) 558, 567, 1398
SatI GCNGC 2 cut(s) 84, 1218
Sau3AI GATC 6 cut(s) 29, 33, 549, 843, 966, 1251
Sau96I GGNCC 1 cut(s) 321
SchI GAGTC 2 cut(s) 369, 482
ScrFI CCNGG 3 cut(s) 859, 1209, 1214
SduI GDGCHC 3 cut(s) 214, 306, 1162
SfaNI GCATC 2 cut(s) 385, 1033
SfuI TTCGAA 1 cut(s) 802
SinI GGWCC 1 cut(s) 321
SmiMI CAYNNNNRTG 4 cut(s) 104, 459, 905, 1136
SpeI ACTAGT 1 cut(s) 133
Sse9I AATT 4 cut(s) 357, 1032, 1098, 1256
SsiI CCGC 3 cut(s) 682, 700, 1290
SspI AATATT 2 cut(s) 165, 1224
SspMI CTAG 1 cut(s) 134
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 3 cut(s) 857, 1207, 1212
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 4 cut(s) 189, 782, 1157, 1310
TaiI ACGT 2 cut(s) 202, 820
TaqI TCGA 3 cut(s) 32, 802, 1084
TaqII GACCGA 1 cut(s) 338
TasI AATT 4 cut(s) 357, 1032, 1098, 1256
TatI WGTACW 1 cut(s) 777
TfiI GAWTC 2 cut(s) 384, 712
Tru1I TTAA 3 cut(s) 558, 567, 1398
Tru9I TTAA 3 cut(s) 558, 567, 1398
TscAI CASTG 2 cut(s) 1033, 1167
TseFI GTSAC 1 cut(s) 1404
TseI GCWGC 2 cut(s) 83, 1217
Tsp45I GTSAC 1 cut(s) 1404
TspDTI ATGAA 7 cut(s) 142, 500, 588, 597, 628, 819, 1197
TspGWI ACGGA 2 cut(s) 286, 544
TspRI CASTG 2 cut(s) 1033, 1167
VneI GTGCAC 1 cut(s) 1158
VpaK11BI GGWCC 1 cut(s) 321
XapI RAATTY 1 cut(s) 1256
XceI RCATGY 3 cut(s) 109, 1241, 1342
XmiI GTMKAC 2 cut(s) 59, 615
XmnI GAANNNNTTC 1 cut(s) 348
XspI CTAG 1 cut(s) 134
Zsp2I ATGCAT 2 cut(s) 15, 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.