pycom09g12850

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012833
Physical Location & Seq
Forward (+)
11380425 .. 11382572
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g12850.2

Sequence Viewer

Length: 699 bp
ATGAACCCGAATAAAACCCTAAACATATATAATCGCAAAGAGAAAAGCAAGCATTTCTGTACTTCAGCAGAGCCAGAAATGATGCTAGAAAACACCATTGCCAAAAGCAAAAAACTGTTTCGAAAAACTCTTGGGAACCTCAAGTCCTTCTTCTGTGGAGGGTACAAGAAACTACCCAAAACCATTTCCTTCAATCCCTTCTATTGTGGGAGAGACCTGAAATATCAGCACCCAACAGATCACTTCTACGCCGATTTTTATGATGAGTGGGAGTCCACTCTCGGTAATTTGACGAAGATAGATAGCGCGCCGGAAGAACCAACAAAGGAAGAAGACCAATGTAGTCCTAAGAAGATCAAACAAGAAGGGGGATTAATGCAGATGAAGCAGACTGGGAGTTCTCATCTACAAAAAGGAGAAGCTCGGGCTTCTTCGCAGAACGCAAATGCAGAAAGCTATACTTTAGCAAGAAGGATGAAGGAGTTTGAAATGGTGGATTCAGGTGATGTGGAACAAGTGCTGGATGTAGAAGAGGCGCTTCACTACTACTCCCGCCTCAAAAGTCCGGCCTACCTTGACATTGTGGACAAGTTCTTCTTGGATATGTACGCAGACTTCTCCATTCCACCAGTGCCTATCAGCGTCAACAATTCAAAGCGGAGACTGAGCTCGCAGAGACTTGGCTCGATGAGGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DEAD_2 Helicase_C_2

Protein Analysis

233

Amino Acids

26.72

Weight (kDa)

8.7

Isoelectric Point (pI)

50.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012833)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G16070
fragaria_vesca FvH4_6g31461
malus_domestica MD09G1210900.v1.1 MD17G1193700.v1.1
prunus_persica Prupe.3G069200_v2.0.a1
pyrus_communis pycom09g12850
rosa_chinensis RchiOBHm_Chr2g0138311
rosa_laevigata RLG00000019733
rosa_multiflora Rmu_sc0002475.1_g000007
rosa_roxburghii Rroxscaffold_2G00106340
rosa_rugosa Rorug02G0351100 Rorug02G0351200
rosa_samantha Rh2AG398800 Rh2BG408800 Rh2BG410200 Rh2CG385600 Rh2DG419100
rosa_wichuraiana Rw2G032500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 308
AciI CCGC 2 cut(s) 553, 658
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 3 cut(s) 61, 164, 608
AgsI TTSAA 3 cut(s) 193, 488, 654
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
AluBI AGCT 3 cut(s) 422, 456, 669
AluI AGCT 3 cut(s) 422, 456, 669
Alw21I GWGCWC 1 cut(s) 671
Alw26I GTCTC 3 cut(s) 207, 655, 670
Ama87I CYCGRG 1 cut(s) 423
AoxI GGCC 1 cut(s) 567
AseI ATTAAT 1 cut(s) 374
AspLEI GCGC 3 cut(s) 308, 310, 538
AsuHPI GGTGA 1 cut(s) 515
AsuII TTCGAA 1 cut(s) 121
AvaI CYCGRG 1 cut(s) 423
BanII GRGCYC 1 cut(s) 671
BarI GAAGNNNNNNTAC 2 cut(s) 599, 631
BbsI GAAGAC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 671
BcoDI GTCTC 3 cut(s) 207, 655, 670
BfaI CTAG 1 cut(s) 86
BfoI RGCGCY 1 cut(s) 539
BmeT110I CYCGRG 1 cut(s) 423
BmiI GGNNCC 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 402
BmsI GCATC 1 cut(s) 72
BmuI ACTGGG 1 cut(s) 402
BpiI GAAGAC 1 cut(s) 339
Bpu14I TTCGAA 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 125
BsaI GGTCTC 1 cut(s) 207
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bse1I ACTGG 2 cut(s) 397, 629
Bse3DI GCAATG 1 cut(s) 96
BseGI GGATG 2 cut(s) 480, 529
BseMI GCAATG 1 cut(s) 96
BseMII CTCAG 1 cut(s) 656
BseNI ACTGG 2 cut(s) 397, 629
BsePI GCGCGC 1 cut(s) 306
Bsh1236I CGCG 1 cut(s) 308
BshFI GGCC 1 cut(s) 569
BsiHKAI GWGCWC 1 cut(s) 671
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 2 cut(s) 311, 566
BsmAI GTCTC 3 cut(s) 207, 655, 670
BsnI GGCC 1 cut(s) 569
Bso31I GGTCTC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 423
Bsp119I TTCGAA 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 671
Bsp143I GATC 2 cut(s) 238, 354
BspACI CCGC 2 cut(s) 553, 658
BspANI GGCC 1 cut(s) 569
BspCNI CTCAG 1 cut(s) 657
BspFNI CGCG 1 cut(s) 308
BspLI GGNNCC 1 cut(s) 137
BspT104I TTCGAA 1 cut(s) 121
BspTNI GGTCTC 1 cut(s) 207
BsrDI GCAATG 1 cut(s) 96
BsrI ACTGG 2 cut(s) 397, 629
BssHII GCGCGC 1 cut(s) 306
BssMI GATC 2 cut(s) 238, 354
Bst4CI ACNGT 1 cut(s) 117
Bst6I CTCTTC 1 cut(s) 525
BstBI TTCGAA 1 cut(s) 121
BstC8I GCNNGC 3 cut(s) 50, 308, 671
BstDEI CTNAG 2 cut(s) 348, 665
BstF5I GGATG 2 cut(s) 480, 529
BstFNI CGCG 1 cut(s) 308
BstH2I RGCGCY 1 cut(s) 539
BstHHI GCGC 3 cut(s) 308, 310, 538
BstKTI GATC 2 cut(s) 241, 357
BstMAI GTCTC 3 cut(s) 207, 655, 670
BstMBI GATC 2 cut(s) 238, 354
BstMWI GCNNNNNNNGC 1 cut(s) 385
BstUI CGCG 1 cut(s) 308
BstV2I GAAGAC 1 cut(s) 339
BsuRI GGCC 1 cut(s) 569
BtsCI GGATG 2 cut(s) 480, 529
BtsIMutI CAGTG 1 cut(s) 636
Cac8I GCNNGC 3 cut(s) 50, 308, 671
CfoI GCGC 3 cut(s) 308, 310, 538
CseI GACGC 1 cut(s) 631
Csp6I GTAC 3 cut(s) 60, 163, 607
CviJI RGCY 7 cut(s) 73, 422, 428, 456, 569, 669, 684
CviKI_1 RGCY 7 cut(s) 73, 422, 428, 456, 569, 669, 684
CviQI GTAC 3 cut(s) 60, 163, 607
DdeI CTNAG 2 cut(s) 348, 665
DpnI GATC 2 cut(s) 240, 356
DpnII GATC 2 cut(s) 238, 354
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
Ecl136II GAGCTC 1 cut(s) 669
Eco24I GRGCYC 1 cut(s) 671
Eco31I GGTCTC 1 cut(s) 207
Eco53kI GAGCTC 1 cut(s) 669
Eco57I CTGAAG 1 cut(s) 48
Eco88I CYCGRG 1 cut(s) 423
EcoICRI GAGCTC 1 cut(s) 669
EcoT38I GRGCYC 1 cut(s) 671
FaiI YATR 6 cut(s) 26, 28, 30, 261, 459, 605
FalI AAGNNNNNCTT 8 cut(s) 134, 166, 445, 477, 522, 554, 581, 613
FauI CCCGC 1 cut(s) 560
FokI GGATG 2 cut(s) 487, 536
FriOI GRGCYC 1 cut(s) 671
FspBI CTAG 1 cut(s) 86
GlaI GCGC 3 cut(s) 307, 309, 537
HaeII RGCGCY 1 cut(s) 539
HaeIII GGCC 1 cut(s) 569
HapII CCGG 2 cut(s) 311, 566
HgaI GACGC 1 cut(s) 631
HhaI GCGC 3 cut(s) 308, 310, 538
Hin6I GCGC 3 cut(s) 306, 308, 536
HinP1I GCGC 3 cut(s) 306, 308, 536
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 2 cut(s) 272, 497
HpaII CCGG 2 cut(s) 311, 566
HphI GGTGA 1 cut(s) 515
Hpy166II GTNNAC 3 cut(s) 276, 586, 646
Hpy8I GTNNAC 3 cut(s) 276, 586, 646
HpyAV CCTTC 6 cut(s) 157, 199, 208, 359, 465, 472
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4V TGCA 2 cut(s) 379, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 385
HpyF3I CTNAG 2 cut(s) 348, 665
HspAI GCGC 3 cut(s) 306, 308, 536
Kzo9I GATC 2 cut(s) 238, 354
LpnPI CCDG 8 cut(s) 87, 230, 324, 378, 486, 506, 579, 642
LweI GCATC 1 cut(s) 72
MaeI CTAG 1 cut(s) 86
MalI GATC 2 cut(s) 240, 356
MboI GATC 2 cut(s) 238, 354
MboII GAAGA 9 cut(s) 142, 307, 326, 341, 344, 364, 423, 542, 586
MhlI GDGCHC 1 cut(s) 671
MluCI AATT 2 cut(s) 286, 649
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 5 cut(s) 149, 152, 526, 566, 684
MseI TTAA 1 cut(s) 374
MspI CCGG 2 cut(s) 311, 566
MvnI CGCG 1 cut(s) 308
MwoI GCNNNNNNNGC 1 cut(s) 385
NdeII GATC 2 cut(s) 238, 354
NlaIV GGNNCC 1 cut(s) 137
NspV TTCGAA 1 cut(s) 121
PauI GCGCGC 1 cut(s) 306
PfeI GAWTC 1 cut(s) 497
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
PshBI ATTAAT 1 cut(s) 374
Psp124BI GAGCTC 1 cut(s) 671
PspN4I GGNNCC 1 cut(s) 137
PteI GCGCGC 1 cut(s) 306
RsaI GTAC 3 cut(s) 61, 164, 608
RsaNI GTAC 3 cut(s) 60, 163, 607
SacI GAGCTC 1 cut(s) 671
SaqAI TTAA 1 cut(s) 374
Sau3AI GATC 2 cut(s) 238, 354
SchI GAGTC 1 cut(s) 281
SduI GDGCHC 1 cut(s) 671
SetI ASST 8 cut(s) 141, 219, 424, 458, 505, 576, 671, 695
SfaNI GCATC 1 cut(s) 72
SfuI TTCGAA 1 cut(s) 121
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 2 cut(s) 286, 649
SsiI CCGC 2 cut(s) 553, 658
SspMI CTAG 1 cut(s) 86
SstI GAGCTC 1 cut(s) 671
TaaI ACNGT 1 cut(s) 117
TaqI TCGA 2 cut(s) 121, 686
TasI AATT 2 cut(s) 286, 649
TatI WGTACW 1 cut(s) 59
TfiI GAWTC 1 cut(s) 497
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TscAI CASTG 1 cut(s) 636
TspDTI ATGAA 3 cut(s) 17, 398, 491
TspRI CASTG 1 cut(s) 636
VspI ATTAAT 1 cut(s) 374
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.